Yan Hu, Buliang Meng, Saige Yin, Meifeng Yang, Yilin Li, Naixin Liu, Shanshan Li, Yixiang Liu, Dandan Sun, Siyu Wang, Yinglei Wang, Zhe Fu, Yutong Wu, Ailan Pang, Jun Sun, Ying Wang, Xinwang Yang
BACKGROUND: Due to the complexity of the mechanisms involved in epileptogenesis, the available antiseizure drugs (ASDs) do not meet clinical needs; hence, both the discovery of new ASDs and the elucidation of novel molecular mechanisms are very important. METHODS: BALB/c mice were utilized to establish an epilepsy model induced by pentylenetetrazol (PTZ) administration. The peptide HsTx2 was administered for treatment. Primary astrocyte culture, immunofluorescence staining, RNA sequencing, identification and quantification of mouse circRNAs, cell transfection, bioinformatics and luciferase reporter analyses, enzyme-linked immunosorbent assay, RNA extraction and reverse transcription-quantitative PCR, Western blot and cell viability assays were used to explore the potential mechanism of HsTx2 via the circ_0001293/miR-8114/TGF-β2 axis...
December 1, 2022: Journal of Neuroinflammation