keyword
MENU ▼
Read by QxMD icon Read
search

Time lapse

keyword
https://www.readbyqxmd.com/read/29351274/dna-polymerase-iv-primarily-operates-outside-of-dna-replication-forks-in-escherichia-coli
#1
Sarah S Henrikus, Elizabeth A Wood, John P McDonald, Michael M Cox, Roger Woodgate, Myron F Goodman, Antoine M van Oijen, Andrew Robinson
In Escherichia coli, damage to the chromosomal DNA induces the SOS response, setting in motion a series of different DNA repair and damage tolerance pathways. DNA polymerase IV (pol IV) is one of three specialised DNA polymerases called into action during the SOS response to help cells tolerate certain types of DNA damage. The canonical view in the field is that pol IV primarily acts at replisomes that have stalled on the damaged DNA template. However, the results of several studies indicate that pol IV also acts on other substrates, including single-stranded DNA gaps left behind replisomes that re-initiate replication downstream of a lesion, stalled transcription complexes and recombination intermediates...
January 19, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29346757/p53-suppresses-metabolic-stress-induced-ferroptosis-in-cancer-cells
#2
Amy Tarangelo, Leslie Magtanong, Kathryn T Bieging-Rolett, Yang Li, Jiangbin Ye, Laura D Attardi, Scott J Dixon
How cancer cells respond to nutrient deprivation remains poorly understood. In certain cancer cells, deprivation of cystine induces a non-apoptotic, iron-dependent form of cell death termed ferroptosis. Recent evidence suggests that ferroptosis sensitivity may be modulated by the stress-responsive transcription factor and canonical tumor suppressor protein p53. Using CRISPR/Cas9 genome editing, small-molecule probes, and high-resolution, time-lapse imaging, we find that stabilization of wild-type p53 delays the onset of ferroptosis in response to cystine deprivation...
January 16, 2018: Cell Reports
https://www.readbyqxmd.com/read/29338469/assessing-efficacy-of-day-3-embryo-time-lapse-algorithms-retrospectively-impacts-of-dataset-type-and-confounding-factors
#3
Yanhe Liu, Katie Feenan, Vincent Chapple, Phillip Matson
This study investigated the efficacy of four published day 3 embryo time-lapse algorithms based on different types of datasets (known implantation data [KID] and single embryo transfer [SET]), and the confounding effect of female age and conventional embryo morphology. Four algorithms were retrospectively applied to three types of datasets generated at Fertility North between February 2013 and December 2014: (a) KID dataset (n = 270), (b) a subset of SET (n = 144, end-point = implantation), and (c) SET (n = 144, end-point = live birth), respectively...
January 16, 2018: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/29337044/lysophosphatidic-acid-regulates-the-motility-of-mcf10ca1a-breast-cancer-cell-sheets-via-two-opposing-signaling-pathways
#4
Christina H Stuelten, Rachel M Lee, Wolfgang Losert, Carole A Parent
Aberrant cell migration leads to the dispersal of malignant cells. The ubiquitous lipid mediator lysophosphatidic acid (LPA) modulates cell migration and is implicated in tumor progression. Yet, the signaling cascades that regulate LPA's effect on cell motility remain unclear. Using time-lapse imaging and quantitative analyses, we studied the role of signaling cascades that act downstream of LPA on the motility of MCF10CA1a breast cancer cells. We found that LPA alters cell motility via two major signaling pathways...
January 11, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29337000/mechanical-forces-program-the-orientation-of-cell-division-during-airway-tube-morphogenesis
#5
Zan Tang, Yucheng Hu, Zheng Wang, Kewu Jiang, Cheng Zhan, Wallace F Marshall, Nan Tang
Oriented cell division plays a key role in controlling organogenesis. The mechanisms for regulating division orientation at the whole-organ level are only starting to become understood. By combining 3D time-lapse imaging, mouse genetics, and mathematical modeling, we find that global orientation of cell division is the result of a combination of two types of spindles with distinct spindle dynamic behaviors in the developing airway epithelium. Fixed spindles follow the classic long-axis rule and establish their division orientation before metaphase...
January 9, 2018: Developmental Cell
https://www.readbyqxmd.com/read/29336778/damage-induced-reactive-oxygen-species-regulate-vimentin-and-dynamic-collagen-based-projections-to-mediate-wound-repair
#6
Danny LeBert, Jayne M Squirrell, Chrissy Freisinger, Julie Rindy, Netta Golenberg, Grace Frecentes, Angela Gibson, Kevin W Eliceiri, Anna Huttenlocher
Tissue injury leads to early wound-associated reactive oxygen species (ROS) production that mediate tissue regeneration. To identify mechanisms that function downstream of redox signals that modulate regeneration, a vimentin reporter of mesenchymal cells was generated by driving GFP from the vimentin promoter in zebrafish. Early redox signaling mediated vimentin reporter activity at the wound margin. Moreover, both ROS and vimentin were necessary for collagen production and reorganization into projections at the leading edge of the wound...
January 16, 2018: ELife
https://www.readbyqxmd.com/read/29336219/asymmetry-between-sister-cells-of-pluripotent-stem-cells-at-the-onset-of-differentiation
#7
Shogo Nakamura, Atsushi Maruyama, Yuki Kondo, Ayumu Kano, Olga Maria De Sousa, Masahiro Iwahashi, Bayar Hexig, Toshihiro Akaike, Jingyue Li, Yohei Hayashi, Kiyoshi Ohnuma
Various somatic stem cells divide asymmetrically; however, it is not known whether embryonic stem cells (ESCs) divide symmetrically or asymmetrically, not only while maintaining an undifferentiated state, but also at the onset of differentiation. Here, we observed single ESCs using time-lapse imaging, and compared sister cell pairs derived from the same mother cell in either the maintenance or differentiation medium. Mouse ESCs were cultured on E-cadherin-coated glass-based dishes, which allowed us to trace single cells...
January 16, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29335064/real-time-assessment-of-cytosolic-mitochondrial-and-nuclear-calcium-levels-change-in-rat-pheochromocytoma-cells-during-pulsed-microwave-exposure-using-a-genetically-encoded-calcium-indicator
#8
Shao Hua Hu, Hui Wang, Li Lu, Hong Mei Zhou, Chang Zhen Wang, Ya Bing Gao, Ji Dong, Rui Yun Peng
Little information is available about the effects of exposure to pulsed microwaves on neuronal Ca2+ signaling under non-thermal conditions. In this study, rat pheochromocytoma (PC12) cells were exposed to pulsed microwaves for 6 min at a specific absorption rate (SAR) of 4 W/kg to assess possible real-time effects. During microwave exposure, free calcium dynamics in the cytosol, mitochondria, and nucleus of cells were monitored by time-lapse microfluorimetry using a genetically encoded calcium indicator (ratiometric-pericam, ratiometric-pericam-mt, and ratiometric-pericam-nu)...
December 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/29331237/time-lapse-algorithms-and-morphological-selection-of-day-5-embryos-for-transfer-a-preclinical-validation-study
#9
Ashleigh Storr, Christos Venetis, Simon Cooke, Suha Kilani, William Ledger
OBJECTIVE: To determine the agreement between published time-lapse algorithms in selecting the best day-5 embryo for transfer, as well as the agreement between these algorithms and embryologists. DESIGN: Prospective study. SETTING: Private in vitro fertilization center. PATIENT(S): Four hundred and twenty-eight embryos from 100 cycles cultured in the EmbryoScope. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Interalgorithm agreement as assessed by the Fleiss kappa coefficient...
January 11, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29330844/bradykinin-mediates-the-association-of-collecting-duct-cells-to-form-migratory-colonies-through-b2-receptor-activation
#10
Edith Del Valle Guaytima, Yamila Romina Brandán, Nicolás Octavio Favale, Bruno Jaime Santacreu, Norma B Sterin-Speziale, María Gabriela Márquez
It is known that bradykinin (BK) B2 receptor (B2R) is expressed in the collecting duct (CD) cells of the newborn rat kidney, but little is known about its role during early postnatal life. Therefore, we hypothesize that BK could participate in the mechanisms that mediate CD formation during the postnatal renal development. Performing primary cultures, combined with biochemical, immunocytochemical, and time-lapse analysis, we studied the role of BK in CD cell behaviour isolated from renal papilla of neonatal rats...
January 13, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29329297/automated-multivariate-analysis-of-multi-sensor-data-submitted-online-real-time-environmental-monitoring
#11
Ingvar Eide, Frank Westad
A pilot study demonstrating real-time environmental monitoring with automated multivariate analysis of multi-sensor data submitted online has been performed at the cabled LoVe Ocean Observatory located at 258 m depth 20 km off the coast of Lofoten-Vesterålen, Norway. The major purpose was efficient monitoring of many variables simultaneously and early detection of changes and time-trends in the overall response pattern before changes were evident in individual variables. The pilot study was performed with 12 sensors from May 16 to August 31, 2015...
2018: PloS One
https://www.readbyqxmd.com/read/29325109/estimating-adolescent-sleep-need-using-dose-response-modelling
#12
Michelle A Short, Nathan Weber, Chelsea Reynolds, Scott Coussens, Mary A Carskadon
Study Objectives: This study will, (a) estimate the nightly sleep need of human adolescents, (b) determine the time course and severity of sleep-related deficits when sleep is reduced below this optimal quantity, and (c) determine whether sleep restriction perturbs the circadian system as well as the sleep homeostat. Methods: Thirty-four adolescents aged 15 to 17 years spent 10 days and 9 nights in the sleep laboratory. Between two baseline nights and two recovery nights with 10-hours' time in bed (TIB) per night, participants experienced either severe sleep restriction (5-hr TIB), moderate sleep restriction (7...
January 6, 2018: Sleep
https://www.readbyqxmd.com/read/29324066/systematic-review-and-meta-analysis-of-the-effects-of-caffeine-in-fatigued-shift-workers-implications-for-emergency-medical-services-personnel
#13
Jennifer L Temple, David Hostler, Christian Martin-Gill, Charity G Moore, Patricia M Weiss, Denisse J Sequeira, Joseph P Condle, Eddy S Lang, J Stephen Higgins, P Daniel Patterson
BACKGROUND: Emergency Medical Services (EMS) workers may experience fatigue as a consequence of shift work. We reviewed the literature to determine the impact of caffeine as a countermeasure to fatigue in EMS personnel and related shift workers. METHODS: We employed the GRADE methodology to perform a systematic literature review and search multiple databases for research that examined the impact of caffeine on outcomes of interest, such as patient and EMS personnel safety...
January 11, 2018: Prehospital Emergency Care
https://www.readbyqxmd.com/read/29322460/real-time-digital-bright-field-technology-for-rapid-antibiotic-susceptibility-testing
#14
Chiara Canali, Erik Spillum, Martin Valvik, Niels Agersnap, Tom Olesen
Optical scanning through bacterial samples and image-based analysis may provide a robust method for bacterial identification, fast estimation of growth rates and their modulation due to the presence of antimicrobial agents. Here, we describe an automated digital, time-lapse, bright field imaging system (oCelloScope, BioSense Solutions ApS, Farum, Denmark) for rapid and higher throughput antibiotic susceptibility testing (AST) of up to 96 bacteria-antibiotic combinations at a time. The imaging system consists of a digital camera, an illumination unit and a lens where the optical axis is tilted 6...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29320486/systematic-characterization-of-maturation-time-of-fluorescent-proteins-in-living-cells
#15
Enrique Balleza, J Mark Kim, Philippe Cluzel
The slow maturation time of fluorescent proteins (FPs) limits the temporal accuracy of measurements of rapid processes such as gene expression dynamics and effectively reduces fluorescence signal in growing cells. We used high-precision time-lapse microscopy to characterize the maturation kinetics of 50 FPs that span the visible spectrum at two different temperatures in Escherichia coli cells. We identified fast-maturing FPs from this set that yielded the highest signal-to-noise ratio and temporal resolution in individual growing cells...
January 2018: Nature Methods
https://www.readbyqxmd.com/read/29319936/submucosal-gland-mucus-strand-velocity-is-decreased-in-chronic-rhinosinusitis
#16
Kiranya E Tipirneni, Shaoyan Zhang, Do-Yeon Cho, Jessica Grayson, Daniel F Skinner, Calvin Mackey, Lindsay Moore, Denzel Cole, Catherine G Banks, Bradford A Woodworth
BACKGROUND: Chronic rhinosinusitis (CRS) may be initiated by innately impaired host defense mechanisms that predispose the upper airways to infection. Recent evidence suggests tethering of submucosal gland mucus strands represents an inciting event within cystic fibrosis (CF) airways, occurring prior to onset of chronic infection. Submucosal gland hypertrophy and defective mucociliary clearance (MCC) are present in actively inflamed sinuses, but mucus strand velocity may also be affected as a secondary event, further contributing to chronic disease...
January 10, 2018: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/29319324/on-the-complex-and-reversible-pathways-of-cdse-quantum-dots-driven-by-pyroelectric-dep
#17
Giuseppe Nasti, Sara Coppola, Federico Olivieri, Veronica Vespini, Vito Pagliarulo, Pietro Ferraro
Electrophoresis (EP) and dielectrophoresis (DEP) are two well established methodologies to manipulate nanoparticles (NPs). Recently, a DEP by virtual electrodes platform was demonstrated on ferroelectric substrates where the driving force is due to the strong electric field generated by pyroelectric effect thus opening new scenarios for manipulating the matter. Such innovative approach named pyroelectric-DEP has several advantages over the traditional EP and DEP. However, a detailed study on this novel approach is required for understanding the complex pathways traced by nanoparticles (NP) under the action of the pyroelectric driven forces and thus for explaining the final patterns...
January 10, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29318551/analysis-of-invasive-activity-of-caf-spheroids-into-three-dimensional-3d-collagen-matrices
#18
María Ángeles Villaronga, Saúl Álvarez Teijeiro, Francisco Hermida-Prado, Marta Garzón-Arango, Victoria Sanz-Moreno, Juana María García-Pedrero
Tumor growth and progression is the result of a complex process controlled not only by malignant cancer cells but also by the surrounding tumor microenvironment (TME). Cancer associated fibroblasts (CAFs), the most abundant cellular component of TME, play an active role in tumor invasion and metastasis by promoting cancer cell invasion through cell-cell interactions and secretion of pro-invasive factors such as extracellular matrix (ECM)-degrading proteases. Due to their tumor-promoting activities, there is an emerging interest in investigating CAFs biology and its potential as drug targets for cancer therapies...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29318550/assessing-the-influence-of-a%C3%A2-protease-in-cell-migration-using-the-barrier-migration-assay
#19
Tania Fontanil, Yamina Mohamedi, Santiago Cal, Álvaro J Obaya
Proteases play crucial roles in all steps of tumor progression including cancer cell migration. In fact, uncontrolled proteolytic activity could lead to the degradation of different components of the extracellular matrix which facilitates dissemination of tumor cells. However, numerous studies have revealed that proteases may also exert tumor-protective actions which could impede progression of malignant cells. Consequently, it is crucial to distinguish those situations in which proteases promote tumor growth from those in which exhibit tumor-suppressive effects...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29317146/momentary-factors-during-marijuana-use-as-predictors-of-lapse-during-attempted-abstinence-in-young-adults
#20
Lydia A Shrier, Vishnudas Sarda, Cassandra Jonestrask, Sion Kim Harris
INTRODUCTION: Young adults using marijuana heavily often try multiple times to quit on their own. We sought to identify momentary experiences during marijuana use that could aid in predicting lapse when young adults subsequently attempt abstinence. METHODS: Young adults (N=34) age 18-25 using marijuana ≥5days/week and planning to quit completed a survey of sociodemographic characteristics, substance use, marijuana expectancies, use motives, perceived social support, and confidence to abstain...
December 29, 2017: Addictive Behaviors
keyword
keyword
54776
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"