keyword
MENU ▼
Read by QxMD icon Read
search

Time lapse

keyword
https://www.readbyqxmd.com/read/28545055/comparative-lytic-efficacy-of-rt-pa-and-ultrasound-in-porcine-versus-human-clots
#1
Shenwen Huang, Himanshu Shekhar, Christy K Holland
INTRODUCTION: Porcine thrombi are employed routinely in preclinical models of ischemic stroke. In this study, we examined the differential lytic susceptibility of porcine and human whole blood clots with and without the use of microbubbles and ultrasound (US) as an adjuvant. MATERIALS AND METHODS: An in vitro system equipped with time-lapse microscopy was used to evaluate recombinant tissue-plasminogen activator (rt-PA) lysis of porcine and human clots in the same species or cross species plasma...
2017: PloS One
https://www.readbyqxmd.com/read/28542717/calibration-of-a-land-subsidence-model-using-insar-data-via-the-ensemble-kalman-filter
#2
Liangping Li, Meijing Zhang, Kurt Katzenstein
The application of interferometric synthetic aperture radar (InSAR) has been increasingly used to improve capabilities to model land subsidence in hydrogeologic studies. A number of investigations over the last decade show how spatially detailed time-lapse images of ground displacements could be utilized to advance our understanding for better predictions. In this work, we use simulated land subsidences as observed measurements, mimicking InSAR data to inversely infer inelastic specific storage in a stochastic framework...
May 23, 2017: Ground Water
https://www.readbyqxmd.com/read/28542418/effects-of-histatin-1-peptide-on-human-corneal-epithelial-cells
#3
Dhara Shah, Marwan Ali, Deepak Shukla, Sandeep Jain, Vinay Kumar Aakalu
PURPOSE: Ocular surface and corneal epithelial wounds are common and potentially debilitating problems. Ideal treatments for these injuries would promote epithelial healing without inflammation, infection and scarring. In addition the best treatments would be cost-efficient, effective, non-toxic and easily applied. Histatin-1 peptides have been shown to be safe and effective enhancers of epithelial wound healing in other model systems. We sought to determine whether histatin-1 peptides could enhance human corneal epithelial wound healing in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28537600/monitoring-interconversion-between-stereochemical-states-in-single-chirality-transfer-complexes-on-a-platinum-surface
#4
Guillaume Goubert, Yi Dong, Michael N Groves, J-C Lemay, Bjørk Hammer, Peter H McBreen
Elementary steps in enantioselective heterogeneous catalysis take place on the catalyst surface and the targeted synthesis of a desired enantiomer requires the implantation of chiral information at the surface, which can be achieved-for example-by adsorbing chiral molecules. Studies of the structures of complexes formed between adsorbed prochiral reagents and chiral molecules yield information on the forces exerting stereocontrol, but further insight could be gained by studying the dynamics of their interactions...
June 2017: Nature Chemistry
https://www.readbyqxmd.com/read/28536419/the-long-non-coding-rna-linc00152-is-essential-for-cell-cycle-progression-through-mitosis-in-hela-cells
#5
Linda Nötzold, Lukas Frank, Minakshi Gandhi, Maria Polycarpou-Schwarz, Matthias Groß, Manuel Gunkel, Nina Beil, Holger Erfle, Nathalie Harder, Karl Rohr, Jakob Trendel, Jeroen Krijgsveld, Thomas Longerich, Peter Schirmacher, Michael Boutros, Sylvia Erhardt, Sven Diederichs
In recent years, long non-coding RNA (lncRNA) research has identified essential roles of these transcripts in virtually all physiological cellular processes including tumorigenesis, but their functions and molecular mechanisms are poorly understood. In this study, we performed a high-throughput siRNA screen targeting 638 lncRNAs deregulated in cancer entities to analyse their impact on cell division by using time-lapse microscopy. We identified 26 lncRNAs affecting cell morphology and cell cycle including LINC00152...
May 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28535778/exosomes-from-mesenchymal-stem-cells-induce-the-conversion-of-hepatocytes-into-progenitor-oval-cells
#6
Hao-Hsiang Wu, Oscar K Lee
BACKGROUND: We previously reported that mesenchymal stem cells (MSCs) possess therapeutic effects in a murine model of carbon tetrachloride-induced acute liver failure. In the study, we observed that the majority of repopulated hepatocytes were of recipient origin and were adjacent to transplanted MSCs; only a low percentage of repopulated hepatocytes were from transplanted MSCs. The findings indicate that MSCs guided the formation of new hepatocytes. Exosomes are important messengers for paracrine signaling delivery...
May 23, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28535128/low-and-high-let-ionizing-radiation-induces-delayed-homologous-recombination-that-persists-for-two-weeks-before-resolving
#7
Christopher P Allen, Hirokazu Hirakawa, Nakako Izumi Nakajima, Sophia Moore, Jingyi Nie, Neelam Sharma, Mayumi Sugiura, Yuko Hoki, Ryoko Araki, Masumi Abe, Ryuichi Okayasu, Akira Fujimori, Jac A Nickoloff
Genome instability is a hallmark of cancer cells and dysregulation or defects in DNA repair pathways cause genome instability and are linked to inherited cancer predisposition syndromes. Ionizing radiation can cause immediate effects such as mutation or cell death, observed within hours or a few days after irradiation. Ionizing radiation also induces delayed effects many cell generations after irradiation. Delayed effects include hypermutation, hyper-homologous recombination, chromosome instability and reduced clonogenic survival (delayed death)...
May 23, 2017: Radiation Research
https://www.readbyqxmd.com/read/28534806/feature-selection-for-nonstationary-data-application-to-human-recognition-using-medical-biometrics
#8
Majid Komeili, Wael Louis, Narges Armanfard, Dimitrios Hatzinakos
Electrocardiogram (ECG) and transient evoked otoacoustic emission (TEOAE) are among the physiological signals that have attracted significant interest in biometric community due to their inherent robustness to replay and falsification attacks. However, they are time-dependent signals and this makes them hard to deal with in across-session human recognition scenario where only one session is available for enrollment. This paper presents a novel feature selection method to address this issue. It is based on an auxiliary dataset with multiple sessions where it selects a subset of features that are more persistent across different sessions...
May 16, 2017: IEEE Transactions on Cybernetics
https://www.readbyqxmd.com/read/28532990/-catch-22-biosecurity-awareness-interpretation-and-practice-amongst-poultry-catchers
#9
Caroline Millman, Rob Christley, Dan Rigby, Diana Dennis, Sarah J O'Brien, Nicola Williams
Campylobacter contamination of chicken on sale in the UK remains at high levels and has a substantial public health impact. This has prompted the application of many interventions in the supply chain, including enhanced biosecurity measures on-farm. Catching and thinning are acknowledged as threats to the maintenance of good biosecurity, yet the people employed to undertake this critical work (i.e. 'catchers') are a rarely studied group. This study uses a mixed methods approach to investigate catchers' (n=53) understanding of the biosecurity threats posed by the catching and thinning, and the barriers to good biosecurity practice...
June 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28532006/mitochondrial-targeted-fluorescent-probe-with-aie-characteristics-for-bioimaging
#10
Xiaodan Lu, Meirong Hou, Qi Xia, Chenggong Yan, Yikai Xu, Ruiyuan Liu
In this work, a new benzothiazole based mitochondrial tracker, Bth-Mito, is synthesized by with 2-benzothiazoleacetonitrile and 4-(diethylamino)-benzaldehyde. Bth-Mito is weakly fluorescent when dissolved in the good solvent but becomes highly emissive in poor solvents, showing a phenomenon of aggregation-induced emission. Bth-Mito shows the greatest potentials for bioimaging applications in view of low cytotoxicity and high photostability. Bth-Mito could penetrate cells to give stable green fluorescence, even after continuous irradiation, making it suitable candidate for time-lapse and long-term bioimaging application...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28530216/-chilaiditi-syndrome-a-case-report
#11
Sławomir Krzemiński
The abnormality being the result of a certain transposition of the large intestine winding in between diaphragm and the liver, owes it's name to a so called Chilaiditi symptom. This symptom is rarely recognized for at most 0,025-0,28% of the whole population statistics - wise. The Chilaiditi symptom can be examined without any serious (abdominal) pain indication, when conducting the gastrointestinal examination with the implication of the different disorders. When carrying out the diagnostic examination, it is strongly recommended to take notice of patients suffering from the pain in abdominal area, especially in case of the patients with the developed air - structure under the right side copula of the diaphragm...
April 21, 2017: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/28529478/slow-and-fast-responses-two-mechanisms-of-trial-outcome-processing-revealed-by-eeg-oscillations
#12
Nikita A Novikov, Yulia M Nurislamova, Natalia A Zhozhikashvili, Evgenii E Kalenkovich, Anna A Lapina, Boris V Chernyshev
Cognitive control includes maintenance of task-specific processes related to attention, and non-specific regulation of motor threshold. Depending upon the nature of the behavioral tasks, these mechanisms may predispose to different kinds of errors, with either increased or decreased response time (RT) of erroneous responses relative to correct responses. Specifically, slow responses are related to attentional lapses and decision uncertainty, these conditions tending to delay RTs of both erroneous and correct responses...
2017: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/28528631/a-fully-integrated-three-dimensional-fluorescence-to-electron-microscopy-correlative-workflow
#13
Claudia S López, Cedric Bouchet-Marquis, Christopher P Arthur, Jessica L Riesterer, Gregor Heiss, Guillaume Thibault, Lee Pullan, Sunjong Kwon, Joe W Gray
While fluorescence microscopy provides tools for highly specific labeling and sensitive detection, its resolution limit and lack of general contrast has hindered studies of cellular structure and protein localization. Recent advances in correlative light and electron microscopy (CLEM), including the fully integrated CLEM workflow instrument, the FEI CorrSight with MAPS, have allowed for a more reliable, reproducible, and quicker approach to correlate three-dimensional time-lapse confocal fluorescence data, with three-dimensional focused ion beam-scanning electron microscopy data...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28526859/evaluation-of-arteriolar-smooth-muscle-cell-function-in-an-ex-vivo-microvascular-network-model
#14
Jessica M Motherwell, Mohammad S Azimi, Kristine Spicer, Natascha G Alves, Nicholas A Hodges, Jerome W Breslin, Prasad V G Katakam, Walter L Murfee
An emerging challenge in tissue engineering biomimetic models is recapitulating the physiological complexity associated with real tissues. Recently, our laboratory introduced the rat mesentery culture model as an ex vivo experimental platform for investigating the multi-cellular dynamics involved in angiogenesis within an intact microvascular network using time-lapse imaging. A critical question remains whether the vessels maintain their functionality. The objective of this study was to determine whether vascular smooth muscle cells in cultured microvascular networks maintain the ability to constrict...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28521613/how-autophagy-eats-large-mitochondria-autophagosome-formation-coupled-with-mitochondrial-fragmentation
#15
Shun-Ichi Yamashita, Tomotake Kanki
Mitochondrial autophagy (mitophagy) is thought to be a multi-step pathway wherein mitochondria are first divided into small fragments, which are subsequently recognized by the phagophore. DNM1L (dynamin 1 like) plays a pivotal role in mitochondrial division; however, its role in mitophagy remains controversial. In our recent study, we examined the contribution of DNM1L to mitophagy and showed that mitophagy and mitochondrial division occur even in DNM1L-defective cells. Furthermore, time-lapse imaging of mitophagy showed that DNM1L-independent mitochondrial division occurs concomitantly with autophagosome formation...
May 4, 2017: Autophagy
https://www.readbyqxmd.com/read/28517109/the-studies-of-para-and-parb-dynamics-reveal-asymmetry-of-chromosome-segregation-in-mycobacteria
#16
Katarzyna Ginda, Isabella Santi, Djenet Bousbaine, Jolanta Zakrzewska-Czerwińska, Dagmara Jakimowicz, John McKinney
Active segregation of bacterial chromosomes usually involves the action of ParB proteins, which bind in proximity of chromosomal origin (oriC) regions forming nucleoprotein complexes - segrosomes. Newly duplicated segrosomes are moved either uni- or bidirectionally by the action of ATPases - ParA proteins. In Mycobacterium smegmatis the oriC region is located in an off-centred position and newly replicated segrosomes are segregated toward cell poles. The elimination of M. smegmatis ParA and/or ParB leads to chromosome segregation defects...
May 18, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28516173/reconsidering-the-device-in-the-drawer-lapses-as-a-design-opportunity-in-personal-informatics
#17
Daniel A Epstein, Jennifer H Kang, Laura R Pina, James Fogarty, Sean A Munson
People stop using personal tracking tools over time, referred to as the lapsing stage of their tool use. We explore how designs can support people when they lapse in tracking, considering how to design data representations for a person who lapses in Fitbit use. Through a survey of 141 people who had lapsed in using Fitbit, we identified three use patterns and four perspectives on tracking. Participants then viewed seven visual representations of their Fitbit data and seven approaches to framing this data. Participant Fitbit use and perspective on tracking influenced their preference, which we surface in a series of contrasts...
September 12, 2016: Proceedings of the ACM International Conference on Ubiquitous Computing
https://www.readbyqxmd.com/read/28516138/data-on-metabolic-dependent-antioxidant-response-in-the-cardiovascular-tissues-of-living-zebrafish-under-stress-conditions
#18
Emiliano Panieri, Massimo M Santoro
In this article we used transgenic zebrafish lines that express compartment-specific isoforms of the roGFP2-Orp1 and Grx1-roGFP2 biosensors, described in Panieri et al (2017) [1], to test the contribute of the pentose phosphate pathway and of the glutathione biosynthesis in the antioxidant capacity of myocardial and endothelial cells in vivo. The transgenic zebrafish embryos were subdued to metabolic inhibition and subsequently challenged with H2O2 or the redox-cycling agent menadione to respectively mimic acute or chronic oxidative stress...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28514656/endogenous-replication-stress-in-mother-cells-leads-to-quiescence-of-daughter-cells
#19
Mansi Arora, Justin Moser, Harsha Phadke, Ashik Akbar Basha, Sabrina L Spencer
Mammalian cells have two fundamentally different states, proliferative and quiescent, but our understanding of how and why cells switch between these states is limited. We previously showed that actively proliferating populations contain a subpopulation that enters quiescence (G0) in an apparently stochastic manner. Using single-cell time-lapse imaging of CDK2 activity and DNA damage, we now show that unresolved endogenous replication stress in the previous (mother) cell cycle prompts p21-dependent entry of daughter cells into quiescence immediately after mitosis...
May 16, 2017: Cell Reports
https://www.readbyqxmd.com/read/28514061/mapping-intermediate-degradation-products-of-poly-lactic-co-glycolic-acid-in-vitro
#20
Jian Li, Peter Nemes, Ji Guo
There is widespread interest in using absorbable polymers, such as poly(lactic-co-glycolic acid) (PLGA), as components in the design and manufacture of new-generation drug eluting stents (DES). PLGA undergoes hydrolysis to progressively degrade through intermediate chemical entities to simple organic acids that are ultimately absorbed by the human body. Understanding the composition and structure of these intermediate degradation products is critical not only to elucidate polymer degradation pathways accurately, but also to assess the safety and performance of absorbable cardiovascular implants...
May 17, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
keyword
keyword
54776
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"