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Collagen AND PRP

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https://www.readbyqxmd.com/read/28625328/platelet-rich-plasma-for-the-treatment-of-photodamage-of-the-skin-of-the-hands
#1
J O Cabrera-Ramírez, A G Puebla-Mora, A González-Ojeda, D García-Martínez, J A Cortés-Lares, A R Márquez-Valdés, G I Contreras-Hernández, J Bracamontes-Blanco, J A Saucedo Ortiz, C Fuentes-Orozco
BACKGROUND: Platelet-rich plasma (PRP) provides growth factors that stimulate fibroblast activation and induce the synthesis of collagen and other components of the extracellular matrix. The objective of this study was to evaluate the effect of PRP in the treatment of photodamage of the skin of the hands. MATERIAL AND METHODS: This was an experimental study. Eighteen patients with aging of the skin of the dorsum of the hands (type III of the Glogau photoaging scale or class II of the Fitzpatrick wrinkle and elastosis scale) were included...
June 15, 2017: Actas Dermo-sifiliográficas
https://www.readbyqxmd.com/read/28623328/platelet-rich-plasma-induces-post-natal-maturation-of-immature-articular-cartilage-and-correlates-with-loxl1-activation
#2
Yadan Zhang, Ben J Morgan, Rachel Smith, Christopher R Fellows, Catherine Thornton, Martyn Snow, Lewis W Francis, Ilyas M Khan
Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re-express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factor-induced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28622916/osteogenic-potential-of-platelet-rich-plasma-in-dental-stem-cell-cultures
#3
L Otero, N Carrillo, J L Calvo-Guirado, J Villamil, R A Delgado-Ruíz
The purpose of this study was to analyse the potential of platelet-rich plasma (PRP) culture media to induce osteogenic differentiation of periodontal ligament stem cells and dental pulp stem cells compared with four other methods of culture. Both types of cell were collected from 35 healthy patients and cultured in five different media (Dulbecco's modified eagle's medium (DMEM); DMEM and melatonin; DMEM and PRP; DMEM and ascorbic acid 200μmol; DMEM and l-ascorbate 2-phosphate 50μmol). Cells were characterised by flow cytometry...
June 13, 2017: British Journal of Oral & Maxillofacial Surgery
https://www.readbyqxmd.com/read/28587096/the-effect-of-platelet-rich-fibrin-calcium-sulfate-hemihydrate-platelet-rich-plasma-and-resorbable-collagen-on-soft-tissue-closure-of-extraction-sites
#4
Lisa M Yerke, Amal Jamjoom, Talal M Zahid, Robert E Cohen
Rapid and complete soft tissue healing after tooth extraction minimizes surgical complications and facilitates subsequent implant placement. We used four treatment methods and assessed changes in soft tissue socket closure following tooth extraction in humans. The effects of platelet-rich fibrin-calcium sulfate hemihydrate (PRF-CSH), platelet-rich plasma-calcium sulfate hemihydrate (PRP-CSH), a resorbable collagen dressing (RCD), and no grafting material were compared in a randomized, controlled pilot study with a blinded parallel design (N = 23)...
May 25, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/28576047/optimally-designed-collagen-polycaprolactone-biocomposites-supplemented-with-controlled-release-of-ha-tcp-rhbmp-2-and-ha-tcp-prp-for-hard-tissue-regeneration
#5
WonJin Kim, Chul Ho Jang, GeunHyung Kim
Collagen has been widely used as a very promising material to regenerate various tissues. It is a chief component of the extracellular matrix, and encourages various biological effects conducive to tissue regeneration. However, poor mechanical stability, low processability, and high level of water absorption can lead to impaired control of growth factor release and have impeded the use of collagen as a functional biomedical scaffold. Here, to overcome the shortcomings of collagen scaffolds, we have additively manufactured collagen/polycaprolactone (PCL) biocomposites supplemented with a bioceramic (hydroxyapatite (HA)/β-tricalcium-phosphate (TCP)) and two growth factors (recombinant human bone morphogenetic protein-2 [rhBMP-2] and platelet-rich plasma [PRP])...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28488318/platelet-rich-plasma-enhances-the-integration-of-bio-engineered-cartilage-with-native-tissue-in-an-in-vitro-model
#6
Corey Sermer, Rita Kandel, Jesse Anderson, Mark Hurtig, John Theodoropoulos
Current therapies for cartilage repair can be limited by an inability of the repair tissue to integrate with host tissue. Thus there is interest in developing approaches to enhance integration. We have previously shown that platelet-rich plasma (PRP) improves cartilage tissue formation. This raised the question as to whether PRP could promote cartilage integration. Chondrocytes were isolated from cartilage harvested from bovine joints, seeded on a porous bone substitute, and grown in vitro to form an osteochondral-like implant...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28485509/biologic-response-of-human-anterior-cruciate-ligamentocytes-on-collagen-patches-to-platelet-rich-plasma-formulations-with-and-without-leucocytes
#7
Anna M Krismer, Romina S Cabra, Rahel D May, Daniela A Frauchiger, Sandro Kohl, Sufian S Ahmad, Benjamin Gantenbein
Due to the poor self-healing capacities of the anterior cruciate ligament, previous primary repair attempts have failed. To enhance biologic healing, platelet rich plasma and collagen scaffold have shown promise in animal models. Platelet rich plasma (PRP) is already used in several clinical applications although outcomes are quite debated. The purpose of this study was to examine the effects of different PRP formulations during 21 days: With leucocytes and pure PRP on human anterior cruciate ligament-derived ligamentocytes grown on collagen patches in 3D cell cultures in vitro...
May 9, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28485099/ectopic-bone-formation-by-aggregated-mesenchymal-stem-cells-from-bone-marrow-and-adipose-tissue-a-comparative-study
#8
Eelco M Fennema, Laurent A H Tchang, Huipin Yuan, Clemens A van Blitterswijk, Ivan Martin, Arnaud Scherberich, Jan de Boer
Tissue engineered constructs (TECs) based on spheroids of bone marrow mesenchymal stromal cells (BM-MSCs) combined with calcium phosphate (CaP) micro-particles and enveloped in a platelet-rich-plasma (PRP) hydrogel showed that aggregation of MSCs improves their ectopic bone formation potential. The stromal vascular fraction (SVF) and adipose-derived MSCs (ASCs) have been recognized as interesting MSC source for bone tissue engineering but their ectopic bone formation is limited. We investigated whether aggregation of ASCs could similarly improve ectopic bone formation by ASCs and SVF cells...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28453295/postinjury-exercise-and-platelet-rich-plasma-therapies-improve-skeletal-muscle-healing-in-rats-but-are-not-synergistic-when-combined
#9
Paola Contreras-Muñoz, Joan Ramon Torrella, Xavier Serres, David Rizo-Roca, Meritxell De la Varga, Ginés Viscor, Vicente Martínez-Ibáñez, José Luis Peiró, Tero A H Järvinen, Gil Rodas, Mario Marotta
BACKGROUND: Skeletal muscle injuries are the most common sports-related injury and a major concern in sports medicine. The effect of platelet-rich plasma (PRP) injections on muscle healing is still poorly understood, and current data are inconclusive. PURPOSE: To evaluate the effects of an ultrasound-guided intramuscular PRP injection, administered 24 hours after injury, and/or posttraumatic daily exercise training for 2 weeks on skeletal muscle healing in a recently established rat model of skeletal muscle injury that highly mimics the muscle trauma seen in human athletes...
April 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28450316/the-combined-use-of-kartogenin-and-platelet-rich-plasma-promotes-fibrocartilage-formation-in-the-wounded-rat-achilles-tendon-entheses
#10
J Zhang, T Yuan, N Zheng, Y Zhou, M V Hogan, J H-C Wang
OBJECTIVES: After an injury, the biological reattachment of tendon to bone is a challenge because healing takes place between a soft (tendon) and a hard (bone) tissue. Even after healing, the transition zone in the enthesis is not completely regenerated, making it susceptible to re-injury. In this study, we aimed to regenerate Achilles tendon entheses (ATEs) in wounded rats using a combination of kartogenin (KGN) and platelet-rich plasma (PRP). METHODS: Wounds created in rat ATEs were given three different treatments: kartogenin platelet-rich plasma (KGN-PRP); PRP; or saline (control), followed by histological and immunochemical analyses, and mechanical testing of the rat ATEs after three months of healing...
April 2017: Bone & Joint Research
https://www.readbyqxmd.com/read/28448011/turbidimetry-on-human-washed-platelets-the-effect-of-the-pannexin1-inhibitor-brilliant-blue-fcf-on-collagen-induced-aggregation
#11
Filippo Molica, Séverine Nolli, Pierre Fontana, Brenda Renata Kwak
Turbidimetry is a laboratory technique that is applied to measure the aggregation of platelets suspended in either plasma (platelet-rich plasma, PRP) or in buffer (washed platelets), by the use of one or a combination of agonists. The use of washed platelets separated from their plasma environment and in the absence of anticoagulants allows for studying intrinsic platelet properties. Among the large panel of agonists, arachidonic acid (AA), adenosine di-phosphate (ADP), thrombin and collagen are the most frequently used...
April 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28355770/-effects-and-mechanism-of-allogeneic-platelet-rich-plasma-on-collagen-synthesis-in-wound-healing
#12
F C Chen, M C Chen, T T Yan, J J Hou, J G Yang
Objective: To investigate the effects and mechanism of allogeneic platelet rich plasma (PRP) on collagen in wound surface at different time. Methods: A total of 50 clean 7-week rats were selected for this study, including 10 rats for platelet-rich blood plasma preparation, 20 rats for PRP group and 20 rats for control group, 0.1 ml allogenic PRP and 0.1 ml saline were smeared respectively on wound surfaces of PRP and control group, wound regeneration and healing were examined. Cellular and histological morphology alteration was observed via Masson staining, type Ⅰ and type Ⅲ collagen protein and mRNA expression level were detected by Western blot and real-time PCR...
April 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/28339078/comparative-evaluation-of-the-effects-of-platelet%C3%A2-rich-plasma-formulations-on-extracellular-matrix-formation-and-the-nf%C3%A2-%C3%AE%C2%BAb-signaling-pathway-in-human-articular-chondrocytes
#13
Wenjing Yin, Haitao Xu, Jiagen Sheng, Zhengliang Xu, Xuetao Xie, Changqing Zhang
Concentrated leukocytes in leukocyte and platelet‑rich plasma (L‑PRP) may deliver increased levels of pro‑inflammatory cytokines to activate the nuclear factor (NF)‑κB signaling pathway, to counter or overwhelm the beneficial effects of growth factors on cartilage regeneration. However, to date, no relevant studies have substantiated this. In the present study, L‑PRP and pure platelet‑rich plasma (P‑PRP) were prepared, and leukocytes, platelets, pro‑inflammatory cytokines and growth factor concentrations were quantified; they were then used to treat human articular chondrocytes (HACs)...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28321425/adult-cells-combined-with-platelet-rich-plasma-for-tendon-healing-cell-source-options
#14
Eva Rubio-Azpeitia, Pello Sánchez, Diego Delgado, Isabel Andia
BACKGROUND: The combination of cells with platelet-rich plasma (PRP) may fulfill tendon deficits and help overcome the limited ability of tendons to heal. PURPOSE: To examine the suitability of 3 human cell types in combination with PRP and the potential impact of the tenocyte-conditioned media (CM) to enhance tendon healing. STUDY DESIGN: Controlled laboratory study. METHODS: Tenocytes, bone marrow-derived mesenchymal stem cells, and skin fibroblasts were cultured in 3-dimensional PRP hydrogels supplemented or not with CM, and cell proliferation and migration were examined...
February 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28315407/controlled-delivery-of-platelet-derived-proteins-enhances-porcine-wound-healing
#15
Daniel W Long, Noah R Johnson, Eric M Jeffries, Hidetaka Hara, Yadong Wang
Platelet-rich plasma (PRP) is widely used for many clinical indications including wound healing due to the high concentrations of growth factors. However, the short half-life of these therapeutic proteins requires multiple large doses, and their efficacy is highly debated among clinicians. Here we report a method of protecting these proteins and releasing them in a controlled manner via a heparin-based coacervate delivery vehicle to improve wound healing in a porcine model. Platelet-derived proteins incorporated into the coacervate were protected and slowly released over 3weeks in vitro...
May 10, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28301205/intratendon-delivery-of-leukocyte-poor-platelet-rich-plasma-improves-healing-compared-with-leukocyte-rich-platelet-rich-plasma-in-a-rabbit-achilles-tendinopathy-model
#16
Ruijian Yan, Yanjia Gu, Jisheng Ran, Yejun Hu, Zefeng Zheng, Mengfeng Zeng, Boon Chin Heng, Xiao Chen, Zi Yin, Weishan Chen, Weiliang Shen, Hongwei Ouyang
BACKGROUND: Chronic tendinopathy is a commonly occurring clinical problem that affects both athletes and inactive middle-aged patients. Although some studies have shown that different platelet-rich plasma (PRP) preparations could exert various therapeutic effects in vitro, the role of leukocytes in PRP has not yet been defined under tendinopathy conditions in vivo. PURPOSE: This study compared the effects of the intratendon delivery of leukocyte-poor PRP (Lp-PRP) versus leukocyte-rich PRP (Lr-PRP) in a rabbit chronic tendinopathy model in vivo...
March 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28246352/evaluation-of-subcutaneous-infiltration-of-autologous-platelet-rich-plasma-on-skin-wound-healing-in-dogs
#17
Haithem A Farghali, Naglaa A AbdElKader, Marwa S Khattab, Huda O Abubakr
Background: Platelet-rich plasma (PRP) is known to be rich in growth factors and cytokines which are crucial to the healing process. Objective: Studying the effect of subcutaneous infiltration of autologous PRP at the wound boundaries on wound epithelization and contraction. Material and Methods: Five adult male mongrel dogs were used. Bilateral acute full-thickness skin wounds (3cm diameter) were created on the thorax symmetrically. Right side wounds were subcutaneously infiltrated with activated PRP at day zero then every week for three consecutive weeks...
February 28, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28217215/the-bone-regeneration-using-bone-marrow-stromal-cells-with-moderate-concentration-platelet-rich-plasma-in-femoral-segmental-defect-of-rats
#18
Junichi Yamakawa, Junichi Hashimoto, Mitsuo Takano, Michiaki Takagi
BACKGROUND: Platelet-rich plasma (PRP) can provide an assortment of growth factors, but how PRP effects bone regeneration is still unknown. The aim of the study was to explore an optimal method of using PRP and bone marrow stromal cells (BMSCs). METHODS: An in vitro experiment was first conducted to determine an appropriate quantity of PRP. BMSCs were cultured with PRP of different concentrations to assess cell proliferation and osteogenic differentiation. Following the in vitro study, a rat femoral segmental defect model was used...
2017: Open Orthopaedics Journal
https://www.readbyqxmd.com/read/28202394/platelet-rich-plasma-prp-induces-chondroprotection-via-increasing-autophagy-anti-inflammatory-markers-and-decreasing-apoptosis-in-human-osteoarthritic-cartilage
#19
COMPARATIVE STUDY
Mayssam Moussa, Daniel Lajeunesse, George Hilal, Oula El Atat, Gaby Haykal, Rim Serhal, Antonio Chalhoub, Charbel Khalil, Nada Alaaeddine
OBJECTIVES: Autophagy constitutes a defense mechanism to overcome aging and apoptosis in osteoarthritic cartilage. Several cytokines and transcription factors are linked to autophagy and play an important role in the degradative cascade in osteoarthritis (OA). Cell therapy such as platelet rich plasma (PRP) has recently emerged as a promising therapeutic tool for many diseases including OA. However, its mechanism of action on improving cartilage repair remains to be determined. The purpose of this study is to investigate the effect of PRP on osteoarthritic chondrocytes and to elucidate the mechanism by which PRP contributes to cartilage regeneration...
March 1, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28178294/histological-observation-of-a-gelatin-sponge-transplant-loaded-with-bone-marrow-derived-mesenchymal-stem-cells-combined-with-platelet-rich-plasma-in-repairing-an-annulus-defect
#20
Xiang Xu, Jianzhong Hu, Hongbin Lu
OBJECTIVE: To research the histological characteristics of a gelatin sponge transplant loaded with goat BMSCs (bone marrow-derived mesenchymal stem cells) combined with PRP (platelet-rich plasma) in repairing an annulus defect. METHOD: BMSCs were separated from the iliac crest of goats, sub-cultured and identified after the third generation. Then, PRP was obtained using blood from the jugular vein of goats via two degrees of centrifugation. In the animal experiments, the goats were divided into the following three groups: a sham group, an injury group and a therapeutic group...
2017: PloS One
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