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Collagen AND PRP

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https://www.readbyqxmd.com/read/28697880/comparison-of-autologous-bone-marrow-and-adipose-tissue-derived-mesenchymal-stem-cells-and-platelet-rich-plasma-for-treating-surgically-induced-lesions-of-the-equine-superficial-digital-flexor-tendon
#1
A Romero, L Barrachina, B Ranera, A R Remacha, B Moreno, I de Blas, A Sanz, F J Vázquez, A Vitoria, C Junquera, P Zaragoza, C Rodellar
Several therapies have been investigated for equine tendinopathies, but satisfactory long term results have not been achieved consistently and a better understanding of the healing mechanism elicited by regenerative therapies is needed. The aim of this study was to assess the separate effects of autologous bone marrow (BM) and adipose tissue (AT) derived mesenchymal stem cells (MSCs), and platelet rich plasma (PRP), for treating lesions induced in the superficial digital flexor tendon (SDFT) of horses. Lesions were created surgically in both SDFTs of the forelimbs of 12 horses and were treated with BM-MSCs (six tendons), AT-MSCs (six tendons) or PRP (six tendons)...
June 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28694685/differential-effect-of-platelet-rich-plasma-fractions-on-%C3%AE-1-integrin-signaling-collagen-biosynthesis-and-prolidase-activity-in-human-skin-fibroblasts
#2
Tomasz Guszczyn, Arkadiusz Surażyński, Ilona Zaręba, Edyta Rysiak, Janusz Popko, Jerzy Pałka
The study was conducted to evaluate the effects of platelet-rich plasma (PRP), supernatant of PRP (SPRP) obtained by centrifugation, and supernatant of activated PRP (SActi-PRP) obtained by Ca(2+) solution-treated PRP on collagen biosynthesis, prolidase activity, and β1-integrin signaling in cultured human skin fibroblasts. Incubation of fibroblasts with 5% PRP for 24 h contributed to ~5-fold increase in collagen biosynthesis compared to the control. In the cells treated with 5% of SPRP or SActi-PRP, collagen biosynthesis showed a 3-fold increase of the control...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28692513/viability-and-biomechanics-of-diced-cartilage-blended-with-platelet-rich-plasma-and-wrapped-with-poly-lactic-co-glycolic-acid-membrane
#3
Jun-Lin Liao, Jia Chen, Bin He, Yong Chen, Jia-Qun Xu, Hong-Ju Xie, Feng Hu, Ai-Jun Wang, ChengQun Luo, Qing-Feng Li, Jian-Da Zhou
OBJECTIVES: The objective of this study was to investigate the viability and biomechanics of diced cartilage blended with platelet-rich plasma (PRP) and wrapped with poly (lactic-co-glycolic) acid (PLGA) membrane in a rabbit model. METHODS: A total of 10 New Zealand rabbits were used for the study. Cartilage grafts were harvested from 1 side ear. The grafts were divided into 3 groups for comparison: bare diced cartilage, diced cartilage wrapped with PLGA membrane, and diced cartilage blended with PRP and wrapped with PLGA membrane...
July 7, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28662879/use-of-platelet-rich-plasma-in-endodontic-procedures-in-adults-regeneration-or-repair-a-report-of-3-cases-with-5%C3%A2-years-of-follow-up
#4
José Francisco Gaviño Orduña, Javier Caviedes-Bucheli, María C Manzanares Céspedes, Esther Berástegui Jimeno, Benjamín Martín Biedma, Juan José Segura-Egea, José López-López
INTRODUCTION: Regenerative endodontic procedures (REP) are a promising alternative for necrotic immature teeth in adolescents and children, but very little evidence is available on this alternative in long-lasting necrotic teeth with open apices in adults. REPs are designed to replace damaged structures of the pulp-dentin complex, but no regeneration has been obtained in any of the cases described in necrotic immature permanent teeth with apical periodontitis with histologic results. The results are limited to periapical tissue repair with increasing root length, thickening of the root walls, and apical closure in young patients...
June 26, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28662545/defective-acid-hydrolase-secretion-in-runx1-haplodeficiency-evidence-for-a-global-platelet-secretory-defect
#5
A K Rao, M Poncz
BACKGROUND: RUNX1 haplodeficiency is associated with thrombocytopenia, platelet dysfunction and a predisposition to acute leukaemia. Platelets possess three distinct types of granules and secretory processes involving dense granules (DG), α-granules and vesicles or lysosomes containing acid hydrolases (AH). Dense granules and granule deficiencies have been reported in patients with RUNX1 mutations. Little is known regarding the secretion from AH-containing vesicles. METHODS AND RESULTS: We studied two related patients with a RUNX1 mutation, easy bruising, and mild thrombocytopenia...
June 29, 2017: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/28638679/decrease-of-serum-biomarker-of-type-ii-collagen-degradation-coll2-1-by-intra-articular-injection-of-an-autologous-plasma-rich-platelet-in-patients-with-unilateral-primary-knee-osteoarthritis
#6
Rasha Mohamed Fawzy, Nashwa Ismail Hashaad, Amira Ibrahim Mansour
OBJECTIVE: This study aimed to evaluate the effect of one dose of intra-articular injection of (PRP) in the knee joint on a specific osteoarthritis (OA) serum biomarker of cartilage degeneration, Collagen 2-1 (Coll2-1), over a short period of 3 months. The aim extended to clarify the effect of PRP on the functional status of the osteoarthritic knee joint. MATERIAL AND METHODS: Sixty patients with primary unilateral knee OA were enrolled in this study. They were subdivided according to Kellgren-Lawrence grading scale (KL) into (Group I): including patients with KL grade < 3 and (Group II): including patients with KL grade ≥3...
June 2017: European Journal of Rheumatology
https://www.readbyqxmd.com/read/28625328/platelet-rich-plasma-for-the-treatment-of-photodamage-of-the-skin-of-the-hands
#7
J O Cabrera-Ramírez, A G Puebla-Mora, A González-Ojeda, D García-Martínez, J A Cortés-Lares, A R Márquez-Valdés, G I Contreras-Hernández, J Bracamontes-Blanco, J A Saucedo Ortiz, C Fuentes-Orozco
BACKGROUND: Platelet-rich plasma (PRP) provides growth factors that stimulate fibroblast activation and induce the synthesis of collagen and other components of the extracellular matrix. The objective of this study was to evaluate the effect of PRP in the treatment of photodamage of the skin of the hands. MATERIAL AND METHODS: This was an experimental study. Eighteen patients with aging of the skin of the dorsum of the hands (type III of the Glogau photoaging scale or class II of the Fitzpatrick wrinkle and elastosis scale) were included...
June 15, 2017: Actas Dermo-sifiliográficas
https://www.readbyqxmd.com/read/28623328/platelet-rich-plasma-induces-post-natal-maturation-of-immature-articular-cartilage-and-correlates-with-loxl1-activation
#8
Yadan Zhang, Ben J Morgan, Rachel Smith, Christopher R Fellows, Catherine Thornton, Martyn Snow, Lewis W Francis, Ilyas M Khan
Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re-express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factor-induced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28622916/osteogenic-potential-of-platelet-rich-plasma-in-dental-stem-cell-cultures
#9
L Otero, N Carrillo, J L Calvo-Guirado, J Villamil, R A Delgado-Ruíz
The purpose of this study was to analyse the potential of platelet-rich plasma (PRP) culture media to induce osteogenic differentiation of periodontal ligament stem cells and dental pulp stem cells compared with four other methods of culture. Both types of cell were collected from 35 healthy patients and cultured in five different media (Dulbecco's modified eagle's medium (DMEM); DMEM and melatonin; DMEM and PRP; DMEM and ascorbic acid 200μmol; DMEM and l-ascorbate 2-phosphate 50μmol). Cells were characterised by flow cytometry...
June 13, 2017: British Journal of Oral & Maxillofacial Surgery
https://www.readbyqxmd.com/read/28603214/enhancement-of-abdominal-wall-defect-repair-using-allogenic-platelet-rich-plasma-with-commercial-polyester-cotton-fabric-damour-in-a-canine-model
#10
Khaled Abouelnasr, Mohamed Hamed, Samah Lashen, Mohamed El Adl, Rasha Eltaysh, Michihito Tagawa
Platelet-rich plasma (PRP) has an important role in musculoskeletal surgery; however, it has been underutilized for accelerating the healing of abdominal wall defects in veterinary practice. Therefore, the aim of this study was to evaluate the use of commercial polyester/cotton fabric (Damour) as a new composite mesh for the repair of experimentally induced abdominal wall defects in canine models, and to investigate the possible role of PRP for improving such repair and reducing allied complications. For this purpose, abdominal wall defects were created in 24 healthy mongrel dogs and then repaired with mesh alone (control group) or mesh and allogenic PRP (PRP group)...
June 11, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28587096/the-effect-of-platelet-rich-fibrin-calcium-sulfate-hemihydrate-platelet-rich-plasma-and-resorbable-collagen-on-soft-tissue-closure-of-extraction-sites
#11
Lisa M Yerke, Amal Jamjoom, Talal M Zahid, Robert E Cohen
Rapid and complete soft tissue healing after tooth extraction minimizes surgical complications and facilitates subsequent implant placement. We used four treatment methods and assessed changes in soft tissue socket closure following tooth extraction in humans. The effects of platelet-rich fibrin-calcium sulfate hemihydrate (PRF-CSH), platelet-rich plasma-calcium sulfate hemihydrate (PRP-CSH), a resorbable collagen dressing (RCD), and no grafting material were compared in a randomized, controlled pilot study with a blinded parallel design (N = 23)...
May 25, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/28576047/optimally-designed-collagen-polycaprolactone-biocomposites-supplemented-with-controlled-release-of-ha-tcp-rhbmp-2-and-ha-tcp-prp-for-hard-tissue-regeneration
#12
WonJin Kim, Chul Ho Jang, GeunHyung Kim
Collagen has been widely used as a very promising material to regenerate various tissues. It is a chief component of the extracellular matrix, and encourages various biological effects conducive to tissue regeneration. However, poor mechanical stability, low processability, and high level of water absorption can lead to impaired control of growth factor release and have impeded the use of collagen as a functional biomedical scaffold. Here, to overcome the shortcomings of collagen scaffolds, we have additively manufactured collagen/polycaprolactone (PCL) biocomposites supplemented with a bioceramic (hydroxyapatite (HA)/β-tricalcium-phosphate (TCP)) and two growth factors (recombinant human bone morphogenetic protein-2 [rhBMP-2] and platelet-rich plasma [PRP])...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28488318/platelet-rich-plasma-enhances-the-integration-of-bio-engineered-cartilage-with-native-tissue-in-an-in-vitro-model
#13
Corey Sermer, Rita Kandel, Jesse Anderson, Mark Hurtig, John Theodoropoulos
Current therapies for cartilage repair can be limited by an inability of the repair tissue to integrate with host tissue. Thus there is interest in developing approaches to enhance integration. We have previously shown that platelet-rich plasma (PRP) improves cartilage tissue formation. This raised the question as to whether PRP could promote cartilage integration. Chondrocytes were isolated from cartilage harvested from bovine joints, seeded on a porous bone substitute, and grown in vitro to form an osteochondral-like implant...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28485509/biologic-response-of-human-anterior-cruciate-ligamentocytes-on-collagen-patches-to-platelet-rich-plasma-formulations-with-and-without-leucocytes
#14
Anna M Krismer, Romina S Cabra, Rahel D May, Daniela A Frauchiger, Sandro Kohl, Sufian S Ahmad, Benjamin Gantenbein
Due to the poor self-healing capacities of the anterior cruciate ligament, previous primary repair attempts have failed. To enhance biologic healing, platelet rich plasma and collagen scaffold have shown promise in animal models. Platelet rich plasma (PRP) is already used in several clinical applications although outcomes are quite debated. The purpose of this study was to examine the effects of different PRP formulations during 21 days: With leucocytes and pure PRP on human anterior cruciate ligament-derived ligamentocytes grown on collagen patches in 3D cell cultures in vitro...
May 9, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28485099/ectopic-bone-formation-by-aggregated-mesenchymal-stem-cells-from-bone-marrow-and-adipose-tissue-a-comparative-study
#15
Eelco M Fennema, Laurent A H Tchang, Huipin Yuan, Clemens A van Blitterswijk, Ivan Martin, Arnaud Scherberich, Jan de Boer
Tissue engineered constructs (TECs) based on spheroids of bone marrow mesenchymal stromal cells (BM-MSCs) combined with calcium phosphate (CaP) micro-particles and enveloped in a platelet-rich-plasma (PRP) hydrogel showed that aggregation of MSCs improves their ectopic bone formation potential. The stromal vascular fraction (SVF) and adipose-derived MSCs (ASCs) have been recognized as interesting MSC source for bone tissue engineering but their ectopic bone formation is limited. We investigated whether aggregation of ASCs could similarly improve ectopic bone formation by ASCs and SVF cells...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28453295/postinjury-exercise-and-platelet-rich-plasma-therapies-improve-skeletal-muscle-healing-in-rats-but-are-not-synergistic-when-combined
#16
Paola Contreras-Muñoz, Joan Ramon Torrella, Xavier Serres, David Rizo-Roca, Meritxell De la Varga, Ginés Viscor, Vicente Martínez-Ibáñez, José Luis Peiró, Tero A H Järvinen, Gil Rodas, Mario Marotta
BACKGROUND: Skeletal muscle injuries are the most common sports-related injury and a major concern in sports medicine. The effect of platelet-rich plasma (PRP) injections on muscle healing is still poorly understood, and current data are inconclusive. PURPOSE: To evaluate the effects of an ultrasound-guided intramuscular PRP injection, administered 24 hours after injury, and/or posttraumatic daily exercise training for 2 weeks on skeletal muscle healing in a recently established rat model of skeletal muscle injury that highly mimics the muscle trauma seen in human athletes...
April 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28450316/the-combined-use-of-kartogenin-and-platelet-rich-plasma-promotes-fibrocartilage-formation-in-the-wounded-rat-achilles-tendon-entheses
#17
J Zhang, T Yuan, N Zheng, Y Zhou, M V Hogan, J H-C Wang
OBJECTIVES: After an injury, the biological reattachment of tendon to bone is a challenge because healing takes place between a soft (tendon) and a hard (bone) tissue. Even after healing, the transition zone in the enthesis is not completely regenerated, making it susceptible to re-injury. In this study, we aimed to regenerate Achilles tendon entheses (ATEs) in wounded rats using a combination of kartogenin (KGN) and platelet-rich plasma (PRP). METHODS: Wounds created in rat ATEs were given three different treatments: kartogenin platelet-rich plasma (KGN-PRP); PRP; or saline (control), followed by histological and immunochemical analyses, and mechanical testing of the rat ATEs after three months of healing...
April 2017: Bone & Joint Research
https://www.readbyqxmd.com/read/28448011/turbidimetry-on-human-washed-platelets-the-effect-of-the-pannexin1-inhibitor-brilliant-blue-fcf-on-collagen-induced-aggregation
#18
Filippo Molica, Séverine Nolli, Pierre Fontana, Brenda Renata Kwak
Turbidimetry is a laboratory technique that is applied to measure the aggregation of platelets suspended in either plasma (platelet-rich plasma, PRP) or in buffer (washed platelets), by the use of one or a combination of agonists. The use of washed platelets separated from their plasma environment and in the absence of anticoagulants allows for studying intrinsic platelet properties. Among the large panel of agonists, arachidonic acid (AA), adenosine di-phosphate (ADP), thrombin and collagen are the most frequently used...
April 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28355770/-effects-and-mechanism-of-allogeneic-platelet-rich-plasma-on-collagen-synthesis-in-wound-healing
#19
F C Chen, M C Chen, T T Yan, J J Hou, J G Yang
Objective: To investigate the effects and mechanism of allogeneic platelet rich plasma (PRP) on collagen in wound surface at different time. Methods: A total of 50 clean 7-week rats were selected for this study, including 10 rats for platelet-rich blood plasma preparation, 20 rats for PRP group and 20 rats for control group, 0.1 ml allogenic PRP and 0.1 ml saline were smeared respectively on wound surfaces of PRP and control group, wound regeneration and healing were examined. Cellular and histological morphology alteration was observed via Masson staining, type Ⅰ and type Ⅲ collagen protein and mRNA expression level were detected by Western blot and real-time PCR...
April 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/28339078/comparative-evaluation-of-the-effects-of-platelet%C3%A2-rich-plasma-formulations-on-extracellular-matrix-formation-and-the-nf%C3%A2-%C3%AE%C2%BAb-signaling-pathway-in-human-articular-chondrocytes
#20
Wenjing Yin, Haitao Xu, Jiagen Sheng, Zhengliang Xu, Xuetao Xie, Changqing Zhang
Concentrated leukocytes in leukocyte and platelet‑rich plasma (L‑PRP) may deliver increased levels of pro‑inflammatory cytokines to activate the nuclear factor (NF)‑κB signaling pathway, to counter or overwhelm the beneficial effects of growth factors on cartilage regeneration. However, to date, no relevant studies have substantiated this. In the present study, L‑PRP and pure platelet‑rich plasma (P‑PRP) were prepared, and leukocytes, platelets, pro‑inflammatory cytokines and growth factor concentrations were quantified; they were then used to treat human articular chondrocytes (HACs)...
May 2017: Molecular Medicine Reports
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