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Collagen AND PRP

Gary W Moore, James C Maloney, Robert A Archer, Kerri L Brown, Katarzyna Mayger, Elaine S Bromidge, Mitra F Najafi
BACKGROUND: Autologous platelet-rich plasma (PRP) is gaining increasing use as a wound healing promoter in a variety of clinical settings, including dentistry. Fresh PRP is often used, necessitating daily draws. The present study investigates the possibility of using stored PRP without having to freeze it by storing PRP under variable conditions and assessing growth factor release as a surrogate marker of continued viability. METHODS: Freshly drawn PRP was stored in oxygen permeable and non-oxygen permeable containers under conditions of constant agitation with or without added prostaglandin, intermittent agitation and no agitation, over an 8-day period...
October 8, 2016: British Journal of Biomedical Science
Anna Osiecka-Iwan, Stanislaw Moskalewski, Anna Hyc
INTRODUCTION: We have previously observed that rat synovial membranes incubated in medium containing cartilage interstitial fluid (CIF) responded by changes in the expression of hyaluronan synthases (HAS1 and HAS2), collagen type I, versican, aggrecan, lubricin, matrix metalloproteinases 2 and 3 (MMP2 and MMP3), tissue inhibitors of metalloproteinases (TIMP1, 2 and 3), transforming growth factor β1 (TGFβ1), tumor necrosis factor (TNF), interleukin (IL) 1β and IL-6. The aim of the study was to evaluate the influence of particular cytokines found in CIF on the gene expression of extracellular matrix (ECM) proteins in synovial membrane cells...
October 3, 2016: Folia Histochemica et Cytobiologica
Ryan Ravi Ramsook, Houman Danesh
The use of platelet rich plasma (PRP) spans across many fields owing to its role in healing and as a natural alternative to surgery. PRP continues to grow however much of the literature is anecdotal or case report based and there is a lack of controlled trials to evaluate standards for PRP. The International Cellular Medical Society (ICMS) has developed guidelines to help with the safe advancement of PRP; however there remains a gap in literature concerning the timing of PRP injections in patients who are on antithrombotic therapy...
September 2016: Pain Physician
Carola Cavallo, Alice Roffi, Brunella Grigolo, Erminia Mariani, Loredana Pratelli, Giulia Merli, Elizaveta Kon, Maurilio Marcacci, Giuseppe Filardo
Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous growth factors (GFs). Platelet activation is a crucial step that might influence the availability of bioactive molecules and therefore tissue healing. Activation of PRP from ten voluntary healthy males was performed by adding 10% of CaCl2, 10% of autologous thrombin, 10% of a mixture of CaCl2 + thrombin, and 10% of collagen type I. Blood derivatives were incubated for 15 and 30 minutes and 1, 2, and 24 hours and samples were evaluated for the release of VEGF, TGF-β1, PDGF-AB, IL-1β, and TNF-α...
2016: BioMed Research International
Nil Guler, Schuharazad Abro, Marty Emanuele, Omer Iqbal, Debra Hoppensteadt, Jawed Fareed
BACKGROUND: Poloxamer-188 (MST-188) is effective in the repair/recovery of damaged cell membranes. MST-188 is a promising agent for protecting blood cell viability. The aim of the study is to test the hypothesis that MST-188 can extend the duration of platelet function. MATERIALS AND METHODS: Blood samples were collected from 20 healthy volunteers. MST-188 (10 or 2 mg/mL) containing platelet-rich plasma (PRP) was prepared with 2 procedures. First, PRP prepared from MST-188 added whole blood (WB); second, MST-188 was added to PRP...
September 20, 2016: Clinical and Applied Thrombosis/hemostasis
Guilherme Akio Tamura Ozaki, Regina Celi Trindade Camargo, Tatiana Emy Koike, Thiago Alves Garcia, Robson Chacon Castoldi, João Domingos Augusto Dos Santos Pereira, Carlos José Leopoldo Constantino, José Carlos Silva Camargo Filho
Treatment of muscle injuries usually results in the interruption of sports practice; thus, studies aimed at accelerating the return to activity, with proper tissue repair, are important. Therefore, this study aimed to evaluate the effects of photobiomodulation (PBM), associated or not with platelet-rich plasma (PRP), on the treatment of muscle injury. Thirty-five animals were used and divided into five groups (n = 7): control (C), control lesion (CL), lesion treated with low-level laser therapy (LLLT) (LLt), lesion treated with PRP (LP), and lesion treated with both techniques, LLLT and PRP (LLtP)...
September 20, 2016: Lasers in Medical Science
Agurne Uribarri, Eneritz Bilbao, Xabier Marichalar-Mendia, Rafael Martínez-Conde, José M Aguirre, Fernando Verdugo
BACKGROUND: Susceptible individuals may be more prone to bone loss after augmentation procedures. PURPOSE: Identify plausible clinical and biological factors influencing apical and marginal bone remodeling at implants placed in augmented sinuses, in patients with and without history of periodontitis. MATERIALS AND METHODS: This prospective cross-sectional clinical study analyzed implant bone levels in a group of 104 patients with and without history of periodontitis undergoing 139 sinus augmentation procedures...
September 15, 2016: Clinical Implant Dentistry and related Research
Chong Teng, Chenhe Zhou, Danfeng Xu, Fanggang Bi
BACKGROUND: The objective of this study was to investigate the potency of platelet-rich plasma (PRP) combined with bone marrow mesenchymal stem cells (BMSCs) to promote tendon-bone healing in a rabbit model. METHODS: In the in vitro study, the effects of PRP on osteogenic induction of BMSCs were analysed. Later, PRP with or without BMSCs was used in the rabbit model of anterior cruciate ligament reconstruction. Specimens were harvested 8 weeks postoperatively to evaluate tendon-bone healing by histology, radiology, and biomechanical testing...
2016: Journal of Orthopaedic Surgery and Research
Oscar Rubini Ávila, Natália Gomes Parizzi, Ana Paula Mayumi Souza, Dayane Silvestre Botini, João Ytimura Alves, Silvio Henrique Maia Almeida
INTRODUCTION: The platelet-rich plasma (PRP) is part of a set of biotechnologies, providing some growth factors that promote repair of different tissues. The polypropylene meshes (PPM) are applied in the correction of abdominal defects, pelvic floor and urinary incontinence, however, they induce many significant complications, as a result of an inappropriate inflammatory response. PURPOSE: To investigate the changes caused by PRP associated with the implantation of PPM in the abdomen of female rabbits, in the production of collagen I and III and the inflammatory infiltrate (ININ)...
September 1, 2016: International Braz J Urol: Official Journal of the Brazilian Society of Urology
Robert-Jan de Vos
Acute and overuse tendon disorders are frequently observed in the middle-aged active population. Tendon overuse injuries are currently designated as "tendinopathy". Histopathological studies have shown that chronic tendinopathy is frequently characterised by degenerative changes, such as decreased organisation of collagen, altered cell distribution and neovascularisation. In the recent years, scientific research and technology in the field of regenerative medicine has provided a new perspectives on managing chronic tendinopathy...
2016: Advances in Experimental Medicine and Biology
Yacouba L Diallo, Véronique Ollivier, Véronique Joly, Dorothée Faille, Giovanna Catalano, Martine Jandrot-Perrus, Antoine Rauch, Patrick Yeni, Nadine Ajzenberg
OBJECTIVES: HIV patients exposed to abacavir have an increased risk of myocardial infarction, with contradictory results in the literature. The aim of our study was to determine whether abacavir has a direct effect on platelet activation and aggregation using platelets from healthy donors and from HIV-infected patients under therapy with an undetectable viral load. METHODS: Platelet-rich plasma (PRP) or whole blood from healthy donors was treated with abacavir (5 or 10 μg/mL) or its active metabolite carbovir diphosphate...
August 11, 2016: Journal of Antimicrobial Chemotherapy
Ahmed M Samy
BACKGROUND: Osteonecrosis of the femoral head (ONFH) is a debilitating disease in orthopedics, frequently progressing to femoral head collapse and osteoarthritis. It is thought to be a multifactorial disease. ONFH ultimately results in femoral head collapse in 75-85% of untreated patients. Total hip arthroplasty (THA) yields satisfactory results in the treatment of the end stage of the disease. However, disease typically affects males between the ages of 20 and 40 years and joint replacement is not the ideal option for younger patients...
July 2016: Indian Journal of Orthopaedics
Alexander Küffer, Asvin K K Lakkaraju, Amit Mogha, Sarah C Petersen, Kristina Airich, Cédric Doucerain, Rajlakshmi Marpakwar, Pamela Bakirci, Assunta Senatore, Arnaud Monnard, Carmen Schiavi, Mario Nuvolone, Bianka Grosshans, Simone Hornemann, Frederic Bassilana, Kelly R Monk, Adriano Aguzzi
Ablation of the cellular prion protein PrP(C) leads to a chronic demyelinating polyneuropathy affecting Schwann cells. Neuron-restricted expression of PrP(C) prevents the disease, suggesting that PrP(C) acts in trans through an unidentified Schwann cell receptor. Here we show that the cAMP concentration in sciatic nerves from PrP(C)-deficient mice is reduced, suggesting that PrP(C) acts via a G protein-coupled receptor (GPCR). The amino-terminal flexible tail (residues 23-120) of PrP(C) triggered a concentration-dependent increase in cAMP in primary Schwann cells, in the Schwann cell line SW10, and in HEK293T cells overexpressing the GPCR Adgrg6 (also known as Gpr126)...
August 25, 2016: Nature
Jorge U Carmona, Diana L Ríos, Catalina López, María E Álvarez, Jorge E Pérez, Mabel E Bohórquez
BACKGROUND: Platelet-rich plasma (PRP) preparations are a common treatment in equine osteoarthritis (OA). However, there are controversies regarding the ideal concentration of platelets and leukocytes in these biological substances necessary to induce an adequate anti-inflammatory and anabolic response in articular cartilage. The aims were to study the influence of leukocyte- and platelet-rich gel (L-PRG) and pure platelet-rich gel (P-PRG) supernatants on the histological changes of cartilage, the degree of chondrocyte apoptosis, the production of hyaluronan (HA) and the gene expression of nuclear factor kappa beta (NFkβ), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1) and cartilage oligomeric matrix protein (COMP) in normal cartilage explants (CEs) challenged with lipopolysaccharide (LPS)...
2016: BMC Veterinary Research
Manle Qiu, Daoyun Chen, Chaoyong Shen, Ji Shen, Huakun Zhao, Yaohua He
Traditional therapeutic methods for skin wounds have many disadvantages, and new wound dressings that can facilitate the healing process are thus urgently needed. Platelet-rich plasma (PRP) contains multiple growth factors (GFs) and shows a significant capacity to heal soft tissue wounds. However, these GFs have a short half-life and deactivate rapidly; we therefore need a sustained delivery system to overcome this shortcoming. In this study, poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide) (PDLLA-PEG-PDLLA: PLEL) hydrogel was successfully created as delivery vehicle for PRP GFs and was evaluated systematically...
2016: International Journal of Molecular Sciences
Lisa A Fortier, Hannah S Chapman, Sarah L Pownder, Brandon L Roller, Jessica A Cross, James L Cook, Brian J Cole
BACKGROUND: Microfracture (MFx) remains a dominant treatment strategy for symptomatic articular cartilage defects. Biologic scaffold adjuncts, such as particulated allograft articular cartilage (BioCartilage) combined with platelet-rich plasma (PRP), offer promise in improving clinical outcomes as an adjunct to MFx. PURPOSE: To evaluate the safety, biocompatibility, and efficacy of BioCartilage and PRP for cartilage repair in a preclinical equine model of full-thickness articular cartilage loss...
September 2016: American Journal of Sports Medicine
Mustafa Girgin, Kenan Binnetoglu, Kazim Duman, Burhan Hakan Kanat, Ziya Cetinkaya, Refik Ayten, Yavuz Selim Ilhan, Necip Ilhan, Ibrahim Seker, Necati Timurkaan
PURPOSE: To evaluate the effects of platelet rich plasma (PRP) on the healing of fascia wherein peritonitis has been created. METHODS: Twenty eight Wistar Albino rats were divided into four groups. Only a primary fascial repair following laparotomy was performed on Group 1, a primary fascial repair performed and PRP treatment applied following laparotomy on Group 2, and a fecal peritonitis created following laparotomy and a primary fascial repair carried out on Group 3...
May 2016: Acta Cirúrgica Brasileira
Qiang Hui, Peng Chang, Bingyu Guo, Yu Zhang, Kai Tao
Ultra-pulsed fractional CO2 laser is an efficient, precise, and safe therapeutic intervention for skin refreshing, although accompanied with prolonged edema and erythema. In recent years, autologous platelet-rich plasma (PRP) has been proven to promote wound and soft tissue healing and collagen regeneration. To investigate whether the combination of PRP and ultra-pulsed fractional CO2 laser had a synergistic effect on therapy for facial rejuvenation. Totally, 13 facial aging females were treated with ultra-pulsed fractional CO2 laser...
June 23, 2016: Rejuvenation Research
Lei Zhang, Shuo Chen, Peng Chang, Nirong Bao, Chao Yang, Yufan Ti, Liwu Zhou, Jianning Zhao
BACKGROUND: Platelet-rich plasma (PRP) is now widely used as a promising treatment for patients with tendinopathy. However, the efficacy of PRP treatment for tendinopathy is controversial mainly because of inconsistent results from human clinical trials and particularly because the concentration and effect of leukocytes in PRP remain largely unknown. HYPOTHESIS: Leukocyte-rich PRP (L-PRP) inhibits growth factor release, decreases proliferation, and induces nontenocyte differentiation of tendon stem cells (TSCs); increases catabolic cytokine concentrations; and causes inflammation and apoptosis...
August 2016: American Journal of Sports Medicine
Eva Rubio-Azpeitia, Ane M Bilbao, Pello Sánchez, Diego Delgado, Isabel Andia
BACKGROUND: Tendinopathies are attributed to failure of the healing process and inadequate tissue remodeling. Plasma injections can trigger regenerative responses by modifying the molecular microenvironment. PURPOSE: To examine the differences in the mitotic, chemotactic, anabolic, and inflammatory effects between leukocyte- and platelet-rich plasma (L-PRP), platelet-rich plasma (PRP), and platelet-poor plasma (PPP). STUDY DESIGN: Controlled laboratory study...
August 2016: American Journal of Sports Medicine
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