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Collagen AND PRP

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https://www.readbyqxmd.com/read/28488318/platelet-rich-plasma-enhances-the-integration-of-bio-engineered-cartilage-with-native-tissue-in-an-in-vitro-model
#1
Corey Sermer, Rita Kandel, Jesse Anderson, Mark Hurtig, John Theodoropoulos
Current therapies for cartilage repair can be limited by an inability of the repair tissue to integrate with host tissue. Thus there is interest in developing approaches to enhance integration. We have previously shown that platelet-rich plasma (PRP) improves cartilage tissue formation. This raised the question as to whether PRP could promote cartilage integration. Chondrocytes were isolated from cartilage harvested from bovine joints, seeded on a porous bone substitute, and grown in vitro to form an osteochondral-like implant...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28485509/the-biologic-response-of-human-anterior-cruciate-ligamentocytes-on-collagen-patches-to-platelet-rich-plasma-formulations-with-and-without-leucocytes
#2
Anna M Krismer, Romina S Cabra, Rahel D May, Daniela A Frauchiger, Sandro Kohl, Sufian S Ahmad, Benjamin Gantenbein
Due to the poor self-healing capacities of the anterior cruciate ligament, previous primary repair attempts have failed. To enhance biologic healing, platelet rich plasma and collagen scaffold have shown promise in animal models. Platelet rich plasma (PRP) is already used in several clinical applications although outcomes are quite debated. The purpose of this study was to examine the effects of different PRP formulations during 21 days: with leucocytes and pure PRP on human anterior cruciate ligament-derived ligamentocytes grown on collagen patches in 3D cell cultures in vitro...
May 9, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28485099/ectopic-bone-formation-by-aggregated-mesenchymal-stem-cells-from-bone-marrow-and-adipose-tissue-a-comparative-study
#3
Eelco M Fennema, Laurent A H Tchang, Huipin Yuan, Clemens A van Blitterswijk, Ivan Martin, Arnaud Scherberich, Jan de Boer
Tissue engineered constructs (TECs) based on spheroids of bone marrow mesenchymal stromal cells (BM-MSCs) combined with calcium phosphate (CaP) micro-particles and enveloped in a platelet-rich-plasma (PRP) hydrogel showed that aggregation of MSCs improves their ectopic bone formation potential. The stromal vascular fraction (SVF) and adipose-derived MSCs (ASCs) have been recognized as interesting MSC source for bone tissue engineering but their ectopic bone formation is limited. We investigated whether aggregation of ASCs could similarly improve ectopic bone formation by ASCs and SVF cells...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28453295/postinjury-exercise-and-platelet-rich-plasma-therapies-improve-skeletal-muscle-healing-in-rats-but-are-not-synergistic-when-combined
#4
Paola Contreras-Muñoz, Joan Ramon Torrella, Xavier Serres, David Rizo-Roca, Meritxell De la Varga, Ginés Viscor, Vicente Martínez-Ibáñez, José Luis Peiró, Tero A H Järvinen, Gil Rodas, Mario Marotta
BACKGROUND: Skeletal muscle injuries are the most common sports-related injury and a major concern in sports medicine. The effect of platelet-rich plasma (PRP) injections on muscle healing is still poorly understood, and current data are inconclusive. PURPOSE: To evaluate the effects of an ultrasound-guided intramuscular PRP injection, administered 24 hours after injury, and/or posttraumatic daily exercise training for 2 weeks on skeletal muscle healing in a recently established rat model of skeletal muscle injury that highly mimics the muscle trauma seen in human athletes...
April 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28450316/the-combined-use-of-kartogenin-and-platelet-rich-plasma-promotes-fibrocartilage-formation-in-the-wounded-rat-achilles-tendon-entheses
#5
J Zhang, T Yuan, N Zheng, Y Zhou, M V Hogan, J H-C Wang
OBJECTIVES: After an injury, the biological reattachment of tendon to bone is a challenge because healing takes place between a soft (tendon) and a hard (bone) tissue. Even after healing, the transition zone in the enthesis is not completely regenerated, making it susceptible to re-injury. In this study, we aimed to regenerate Achilles tendon entheses (ATEs) in wounded rats using a combination of kartogenin (KGN) and platelet-rich plasma (PRP). METHODS: Wounds created in rat ATEs were given three different treatments: kartogenin platelet-rich plasma (KGN-PRP); PRP; or saline (control), followed by histological and immunochemical analyses, and mechanical testing of the rat ATEs after three months of healing...
April 2017: Bone & Joint Research
https://www.readbyqxmd.com/read/28448011/turbidimetry-on-human-washed-platelets-the-effect-of-the-pannexin1-inhibitor-brilliant-blue-fcf-on-collagen-induced-aggregation
#6
Filippo Molica, Séverine Nolli, Pierre Fontana, Brenda Renata Kwak
Turbidimetry is a laboratory technique that is applied to measure the aggregation of platelets suspended in either plasma (platelet-rich plasma, PRP) or in buffer (washed platelets), by the use of one or a combination of agonists. The use of washed platelets separated from their plasma environment and in the absence of anticoagulants allows for studying intrinsic platelet properties. Among the large panel of agonists, arachidonic acid (AA), adenosine di-phosphate (ADP), thrombin and collagen are the most frequently used...
April 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28355770/-effects-and-mechanism-of-allogeneic-platelet-rich-plasma-on-collagen-synthesis-in-wound-healing
#7
F C Chen, M C Chen, T T Yan, J J Hou, J G Yang
Objective: To investigate the effects and mechanism of allogeneic platelet rich plasma (PRP) on collagen in wound surface at different time. Methods: A total of 50 clean 7-week rats were selected for this study, including 10 rats for platelet-rich blood plasma preparation, 20 rats for PRP group and 20 rats for control group, 0.1 ml allogenic PRP and 0.1 ml saline were smeared respectively on wound surfaces of PRP and control group, wound regeneration and healing were examined. Cellular and histological morphology alteration was observed via Masson staining, type Ⅰ and type Ⅲ collagen protein and mRNA expression level were detected by Western blot and real-time PCR...
April 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/28339078/comparative-evaluation-of-the-effects-of-platelet%C3%A2-rich-plasma-formulations-on-extracellular-matrix-formation-and-the-nf%C3%A2-%C3%AE%C2%BAb-signaling-pathway-in-human-articular-chondrocytes
#8
Wenjing Yin, Haitao Xu, Jiagen Sheng, Zhengliang Xu, Xuetao Xie, Changqing Zhang
Concentrated leukocytes in leukocyte and platelet‑rich plasma (L‑PRP) may deliver increased levels of pro‑inflammatory cytokines to activate the nuclear factor (NF)‑κB signaling pathway, to counter or overwhelm the beneficial effects of growth factors on cartilage regeneration. However, to date, no relevant studies have substantiated this. In the present study, L‑PRP and pure platelet‑rich plasma (P‑PRP) were prepared, and leukocytes, platelets, pro‑inflammatory cytokines and growth factor concentrations were quantified; they were then used to treat human articular chondrocytes (HACs)...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28321425/adult-cells-combined-with-platelet-rich-plasma-for-tendon-healing-cell-source-options
#9
Eva Rubio-Azpeitia, Pello Sánchez, Diego Delgado, Isabel Andia
BACKGROUND: The combination of cells with platelet-rich plasma (PRP) may fulfill tendon deficits and help overcome the limited ability of tendons to heal. PURPOSE: To examine the suitability of 3 human cell types in combination with PRP and the potential impact of the tenocyte-conditioned media (CM) to enhance tendon healing. STUDY DESIGN: Controlled laboratory study. METHODS: Tenocytes, bone marrow-derived mesenchymal stem cells, and skin fibroblasts were cultured in 3-dimensional PRP hydrogels supplemented or not with CM, and cell proliferation and migration were examined...
February 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28315407/controlled-delivery-of-platelet-derived-proteins-enhances-porcine-wound-healing
#10
Daniel W Long, Noah R Johnson, Eric M Jeffries, Hidetaka Hara, Yadong Wang
Platelet-rich plasma (PRP) is widely used for many clinical indications including wound healing due to the high concentrations of growth factors. However, the short half-life of these therapeutic proteins requires multiple large doses, and their efficacy is highly debated among clinicians. Here we report a method of protecting these proteins and releasing them in a controlled manner via a heparin-based coacervate delivery vehicle to improve wound healing in a porcine model. Platelet-derived proteins incorporated into the coacervate were protected and slowly released over 3weeks in vitro...
March 14, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28301205/intratendon-delivery-of-leukocyte-poor-platelet-rich-plasma-improves-healing-compared-with-leukocyte-rich-platelet-rich-plasma-in-a-rabbit-achilles-tendinopathy-model
#11
Ruijian Yan, Yanjia Gu, Jisheng Ran, Yejun Hu, Zefeng Zheng, Mengfeng Zeng, Boon Chin Heng, Xiao Chen, Zi Yin, Weishan Chen, Weiliang Shen, Hongwei Ouyang
BACKGROUND: Chronic tendinopathy is a commonly occurring clinical problem that affects both athletes and inactive middle-aged patients. Although some studies have shown that different platelet-rich plasma (PRP) preparations could exert various therapeutic effects in vitro, the role of leukocytes in PRP has not yet been defined under tendinopathy conditions in vivo. PURPOSE: This study compared the effects of the intratendon delivery of leukocyte-poor PRP (Lp-PRP) versus leukocyte-rich PRP (Lr-PRP) in a rabbit chronic tendinopathy model in vivo...
March 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28246352/evaluation-of-subcutaneous-infiltration-of-autologous-platelet-rich-plasma-on-skin-wound-healing-in-dogs
#12
Haithem A Farghali, Naglaa A AbdElKader, Marwa S Khattab, Huda O Abubakr
Background: Platelet-rich plasma (PRP) is known to be rich in growth factors and cytokines which are crucial to the healing process. Objective: Studying the effect of subcutaneous infiltration of autologous PRP at the wound boundaries on wound epithelization and contraction. Material and Methods: Five adult male mongrel dogs were used. Bilateral acute full-thickness skin wounds (3cm diameter) were created on the thorax symmetrically. Right side wounds were subcutaneously infiltrated with activated PRP at day zero then every week for three consecutive weeks...
February 28, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28217215/the-bone-regeneration-using-bone-marrow-stromal-cells-with-moderate-concentration-platelet-rich-plasma-in-femoral-segmental-defect-of-rats
#13
Junichi Yamakawa, Junichi Hashimoto, Mitsuo Takano, Michiaki Takagi
BACKGROUND: Platelet-rich plasma (PRP) can provide an assortment of growth factors, but how PRP effects bone regeneration is still unknown. The aim of the study was to explore an optimal method of using PRP and bone marrow stromal cells (BMSCs). METHODS: An in vitro experiment was first conducted to determine an appropriate quantity of PRP. BMSCs were cultured with PRP of different concentrations to assess cell proliferation and osteogenic differentiation. Following the in vitro study, a rat femoral segmental defect model was used...
2017: Open Orthopaedics Journal
https://www.readbyqxmd.com/read/28202394/platelet-rich-plasma-prp-induces-chondroprotection-via-increasing-autophagy-anti-inflammatory-markers-and-decreasing-apoptosis-in-human-osteoarthritic-cartilage
#14
Mayssam Moussa, Daniel Lajeunesse, George Hilal, Oula El Atat, Gaby Haykal, Rim Serhal, Antonio Chalhoub, Charbel Khalil, Nada Alaaeddine
OBJECTIVES: Autophagy constitutes a defense mechanism to overcome aging and apoptosis in osteoarthritic cartilage. Several cytokines and transcription factors are linked to autophagy and play an important role in the degradative cascade in osteoarthritis (OA). Cell therapy such as platelet rich plasma (PRP) has recently emerged as a promising therapeutic tool for many diseases including OA. However, its mechanism of action on improving cartilage repair remains to be determined. The purpose of this study is to investigate the effect of PRP on osteoarthritic chondrocytes and to elucidate the mechanism by which PRP contributes to cartilage regeneration...
March 1, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28178294/histological-observation-of-a-gelatin-sponge-transplant-loaded-with-bone-marrow-derived-mesenchymal-stem-cells-combined-with-platelet-rich-plasma-in-repairing-an-annulus-defect
#15
Xiang Xu, Jianzhong Hu, Hongbin Lu
OBJECTIVE: To research the histological characteristics of a gelatin sponge transplant loaded with goat BMSCs (bone marrow-derived mesenchymal stem cells) combined with PRP (platelet-rich plasma) in repairing an annulus defect. METHOD: BMSCs were separated from the iliac crest of goats, sub-cultured and identified after the third generation. Then, PRP was obtained using blood from the jugular vein of goats via two degrees of centrifugation. In the animal experiments, the goats were divided into the following three groups: a sham group, an injury group and a therapeutic group...
2017: PloS One
https://www.readbyqxmd.com/read/28150526/evaluation-of-an-automated-light-transmission-aggregometry
#16
Li-Qin Ling, Juan Liao, Qian Niu, Xia Wang, Jin Jia, Cheng-Hua Zuo, Hong Jiang, Jing Zhou
Light transmission aggregometry (LTA) is the "gold standard" for platelet function assessment, but it is time-consuming and labor intensive. Recently, an automated platelet aggregation method has been developed on a routine coagulation analyzer (Sysmex CS-2100i). In this study, the performances of CS-2100i including repeatability, correlation with a reference aggregometer (Chrono-log Model 700), and the threshold limitation of platelet counts in platelet-rich plasma (PRP) were evaluated for clinical use. The agonists were adenosine diphosphate (ADP), arachidonic acid, collagen, epinephrine, and ristocetin...
February 2, 2017: Platelets
https://www.readbyqxmd.com/read/28127950/kartogenin-with-prp-promotes-the-formation-of-fibrocartilage-zone-in-the-tendon-bone-interface
#17
Yiqin Zhou, Jianying Zhang, Jinsong Yang, Manoj Narava, Guangyi Zhao, Ting Yuan, Haishan Wu, Nigel Zheng, MaCalus V Hogan, James H-C Wang
Treatment of tendon-bone junction injuries is a challenge because tendon-bone interface often heals poorly and the fibrocartilage zone, which reduces stress concentration, at the interface is not formed. In this study, we used a compound called kartogenin (KGN) with platelet-rich plasma (PRP) to induce the formation of fibrocartilage zone in a rat tendon graft-bone tunnel model. The experimental rats received KGN-PRP or PRP injections in the tendon graft-bone tunnel interface. The control group received saline...
January 27, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28127660/comparative-study-using-autologous-fat-grafts-plus-platelet-rich-plasma-with-or-without-fractional-co2-laser-resurfacing-in-treatment-of-acne-scars-analysis-of-outcomes-and-satisfaction-with-face-q
#18
S Tenna, A Cogliandro, M Barone, Vincenzo Panasiti, M Tirindelli, Carolina Nobile, Paolo Persichetti
BACKGROUND: A multitude of options are traditionally used for the treatment of acne scars; however, newer treatment modalities are emerging to decrease the propensity for post-inflammatory hyperpigmentation and upregulate new collagen production. The aim of this study was to evaluate the efficacy of nanofat and platelet-rich plasma (PRP) infiltration alone and combined with fractional CO2 laser resurfacing to improve atrophic scars of the face. METHODS: From March 2014 to June 2015, 30 patients with atrophic acne scars on the cheeks were selected for this study...
January 26, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28119114/evaluation-of-platelet-rich-plasma-gel-potential-in-acceleration-of-wound-healing-duration-in-patients-underwent-pilonidal-sinus-surgery-a-randomized-controlled-parallel-clinical-trial
#19
Saeed Mohammadi, Shirzad Nasiri, Mohammad Hossein Mohammadi, Ashraf Malek Mohammadi, Mohsen Nikbakht, Mahdi Zahed Panah, Hiva Safar, Shayan Mostafaei, Amir Hossein Norooznezhad, Ahmad Reza Soroosh, Kamran Alimoghaddam, Ardeshir Ghavamzadeh
OBJECTIVES: One of the most important surgical issues applied in the treatment of pilonidal sinus disease is wound healing. The aim of this study was to investigate the possible effect of platelet-rich plasma (PRP) gel on accelerating wound healing in these patients. METHODS: In this randomized, controlled, parallel group clinical trial, 110 patients were randomly allocated into two parallel groups with the same size (controls and treatment arm) after meeting inclusion and exclusion criteria...
April 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28116973/human-adipose-derived-mesenchymal-stem-cells-for-osseous-rehabilitation-of-induced-osteoradionecrosis-a-rodent-model
#20
Jeffrey R Janus, Ryan S Jackson, Katherine A Lees, Stephen G Voss, Zachary C Wilson, Nicholas B Remmes, Michael G Keeney, Joaquin J Garcia, Serban San Marina
Objective Human adipose-derived mesenchymal stem cells (ADSCs) were used to rehabilitate bone damaged by osteoradionecrosis (ORN) in an established animal model. Study Design Prospective animal study. Setting Academic department laboratory. Subjects and Methods After institutional review board and Institutional Animal Care and Use Committee approval, 24 athymic nude rats were divided into 5 groups: 4 groups irradiated (20 Gy) by brachytherapy catheter placed at the left hemimandible and 1 mock irradiation control (n = 4)...
April 2017: Otolaryngology—Head and Neck Surgery
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