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https://www.readbyqxmd.com/read/27773123/the-role-of-smg1-expression-in-b-cell-lymphoproliferative-disease
#1
Patricia Rebeiro, Alexander James, Silvia Ling, Nicole Caxeiro, C Soon Lee, Tara Roberts
No abstract text is available yet for this article.
February 2016: Pathology
https://www.readbyqxmd.com/read/27571030/simplified-enzymatic-upgrading-of-high-acid-rice-bran-oil-using-ethanol-as-a-novel-acyl-acceptor
#2
Daoming Li, Weifei Wang, Rabia Durrani, Xingxing Li, Bo Yang, Yonghua Wang
One of the major challenges in the upgrading of high-acid rice bran oil (RBO) is to efficiently reduce the amount of free fatty acids. Here we report a novel method for upgrading high-acid RBO using ethanol as a novel acyl acceptor in combination with a highly selective lipase from Malassezia globosa (SMG1-F278N). This process enabled an unprecedented deacidification efficiency of up to 99.80% in a short time (6 h); the immobilized SMG1-F278N used in deacidification exhibited excellent operational stability and could be used for at least 10 consecutive batches without detectable loss in activity...
September 7, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27540752/a-novel-process-for-the-synthesis-of-highly-pure-n-3-polyunsaturated-fatty-acid-pufa-enriched-triglycerides-by-combined-transesterification-and-ethanolysis
#3
Daoming Li, Weifei Wang, Xiaoli Qin, Xingxing Li, Bo Yang, Yonghua Wang
In this study, a novel two-step enzymatic reaction was developed for the synthesis of highly pure triacylglycerols (TAGs) with a high content of n-3 polyunsaturated fatty acids (PUFAs). Glyceride mixtures were primarily synthesized by Novozym 435-catalyzed transesterification of glycerol and DHA/EPA-rich ethyl esters (EEs), followed by removal of partial glycerides, for the first time, by immobilized mono- and diacylglycerol lipase SMG1-F278N-catalyzed ethanolysis. TAG yield as high as 98.66% was achieved under the optimized conditions, and highly pure (98...
August 31, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27419053/lid-mobility-in-lipase-smg1-validated-using-a-thiol-disulfide-redox-potential-probe
#4
Shaohua Guo, Grzegorz Maria Popowicz, Daoming Li, Dongjuan Yuan, Yonghua Wang
Most lipases possess a lid domain above the catalytic site that is responsible for their activation. Lipase SMG1 from Malassezia globose CBS 7966 (Malassezia globosa LIP1), is a mono- and diacylglycerol lipase with an atypical loop-like lid domain. Activation of SMG1 was proposed to be solely through a gating mechanism involving two residues (F278 and N102). However, through disulfide bond cross-linking of the lid, this study shows that full activation also requires mobility of the lid domain, contrary to a previous proposal...
May 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/26841701/the-rna-helicase-dhx34-functions-as-a-scaffold-for-smg1-mediated-upf1-phosphorylation
#5
Roberto Melero, Nele Hug, Andrés López-Perrote, Akio Yamashita, Javier F Cáceres, Oscar Llorca
Nonsense-mediated decay (NMD) is a messenger RNA quality-control pathway triggered by SMG1-mediated phosphorylation of the NMD factor UPF1. In recent times, the RNA helicase DHX34 was found to promote mRNP remodelling, leading to activation of NMD. Here we demonstrate the mechanism by which DHX34 functions in concert with SMG1. DHX34 comprises two distinct structural units, a core that binds UPF1 and a protruding carboxy-terminal domain (CTD) that binds the SMG1 kinase, as shown using truncated forms of DHX34 and electron microscopy of the SMG1-DHX34 complex...
February 4, 2016: Nature Communications
https://www.readbyqxmd.com/read/26365206/structure-of-product-bound-smg1-lipase-active-site-gating-implications
#6
Shaohua Guo, Jinxin Xu, Ioannis V Pavlidis, Dongming Lan, Uwe T Bornscheuer, Jinsong Liu, Yonghua Wang
Monoacylglycerol and diacylglycerol lipases are industrially interesting enzymes, due to the health benefits that arise from the consumption of diglycerides compared to the traditional triglyceride oils. Most lipases possess an α-helix (lid) directly over the catalytic pocket which regulates the activity of the enzyme. Generally, lipases exist in active and inactive conformations, depending on the positioning of this lid subdomain. However, lipase SMG1, a monoacylglycerol and diacylglycerol specific lipase, has an atypical activation mechanism...
December 2015: FEBS Journal
https://www.readbyqxmd.com/read/26239010/the-role-of-residues-103-104-and-278-in-the-activity-of-smg1-lipase-from-malassezia-globosa-a-site-directed-mutagenesis-study
#7
Dongming Lan, Qian Wang, Grzegorz Maria Popowicz, Bo Yang, Qingyun Tang, Yonghua Wang
The SMG1 lipase from Malassezia globosa is a newly found mono- and diacylglycerol (DAG) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. In the present study, we characterized the contribution of three residues, L103 and F104 in the lid and F278 in the rim of the binding site groove, on the function of SMG1 lipase. Sitedirected mutagenesis was conducted at these sites, and each of the mutants was expressed in the yeast Pichia pastoris, purified, and characterized for their activity toward DAG and pnitrophenol (pNP) ester...
November 2015: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/26199121/novel-inhibitor-against-malassezia-globosa-lip1-smg1-a-potential-anti-dandruff-target
#8
Shaohua Guo, Wenkang Huang, Jian Zhang, Yonghua Wang
Compelling evidence have demonstrated the role of lipase activity in the pathogenicity of Malassezia globosa toward dandruff and seborrheic dermatitis (D/SD). As a representative secreted lipase from M. globosa CBS 7966, Malassezia globosa LIP1 (SMG1) is considered a potential anti-dandruff target. In this study, homology modeling, docking-based virtual screening and in vitro lipase-based assay were integrated to identify the first hit compound against SMG1, with an IC50 of 20 μM against synthetic lipase substrate, and of 0...
September 1, 2015: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/25837472/residue-asn277-affects-the-stability-and-substrate-specificity-of-the-smg1-lipase-from-malassezia-globosa
#9
Dongming Lan, Qian Wang, Jinxin Xu, Pengfei Zhou, Bo Yang, Yonghua Wang
Thermostability and substrate specificity are important characteristics of enzymes for industrial application, which can be improved by protein engineering. SMG1 lipase from Malassezia globosa is a mono- and diacylglycerol lipase (MDL) that shows activity toward mono- and diacylglycerols, but no activity toward triacylglycerols. SMG1 lipase is considered a potential biocatalyst applied in oil/fat modification and its crystal structure revealed that an interesting residue-Asn277 may contribute to stabilize loop 273-278 and the 3104 helix which are important to enzyme characterization...
2015: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/25783054/a-mechanistic-study-into-the-epoxidation-of-carboxylic-acid-and-alkene-in-a-mono-di-acylglycerol-lipase
#10
Xuping Wang, Qingyun Tang, Grzegorz Maria Popowicz, Bo Yang, Yonghua Wang
More and more industrial chemistry reactions rely on green technologies. Enzymes are finding increasing use in diverse chemical processes. Epoxidized vegetable oils have recently found applications as plasticizers and additives for PVC production. We report here an unusual activity of the Malassezia globosa lipase (SMG1) that is able to catalyze epoxidation of alkenes. SMG1 catalyzes formation of peroxides from long chain carboxylic acids that subsequently react with double bonds of alkenes to produce epoxides...
May 1, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/25520033/biological-and-clinical-significance-of-epigenetic-silencing-of-marveld1-gene-in-lung-cancer
#11
Ming Shi, Shan Wang, Yuanfei Yao, Yiqun Li, Hao Zhang, Fang Han, Huan Nie, Jie Su, Zeyu Wang, Lei Yue, Jingyan Cao, Yu Li
Epigenetic silence in cancer frequently altered signal-transduction pathways during the early stages of tumor development. Recent progress in the field of cancer epigenetics has led to new opportunities for diagnosis and treatment of cancer. We previously demonstrated that novel identified nuclear factor MARVELD1 was widely expressed in human tissues, but down-regulated by promoter methylation in multiple cancers. This study was carried out to determine the biological and clinical significance of MARVELD1 gene silencing in lung cancer...
2014: Scientific Reports
https://www.readbyqxmd.com/read/25257528/smg1-acts-as-a-novel-potential-tumor-suppressor-with-epigenetic-inactivation-in-acute-myeloid-leukemia
#12
Yahui Du, Fei Lu, Peng Li, Jingjing Ye, Min Ji, Daoxin Ma, Chunyan Ji
Suppressor with morphogenetic effect on genitalia family member (SMG1) belongs to a family of phosphoinositide 3-kinase-related kinases and is the main kinase involved in nonsense-mediated mRNA decay. Recently, SMG1 was suggested as a novel potential tumor suppressor gene, particularly in hypoxic tumors. To investigate the function of SMG1 in acute myeloid leukemia (AML), we performed methylation-specific polymerase chain reaction and found that SMG1 was hypermethylated in the promoter region. SMG1 hypermethylation was found in 66% (33/50) of AML samples compared with none (0/14) of the normal controls...
2014: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/25220460/the-rna-helicase-dhx34-activates-nmd-by-promoting-a-transition-from-the-surveillance-to-the-decay-inducing-complex
#13
Nele Hug, Javier F Cáceres
Nonsense-mediated decay (NMD) is a surveillance mechanism that degrades aberrant mRNAs. A complex comprising SMG1, UPF1, and the translation termination factors eRF1 and eRF3 (SURF) is assembled in the vicinity of a premature termination codon. Subsequently, an interaction with UPF2, UPF3b, and the exon junction complex induces the formation of the decay-inducing complex (DECID) and triggers NMD. We previously identified the RNA helicase DHX34 as an NMD factor in C. elegans and in vertebrates. Here, we investigate the mechanism by which DHX34 activates NMD in human cells...
September 25, 2014: Cell Reports
https://www.readbyqxmd.com/read/25184677/a-post-translational-regulatory-switch-on-upf1-controls-targeted-mrna-degradation
#14
Tatsuaki Kurosaki, Wencheng Li, Mainul Hoque, Maximilian W-L Popp, Dmitri N Ermolenko, Bin Tian, Lynne E Maquat
Nonsense-mediated mRNA decay (NMD) controls the quality of eukaryotic gene expression and also degrades physiologic mRNAs. How NMD targets are identified is incompletely understood. A central NMD factor is the ATP-dependent RNA helicase upframeshift 1 (UPF1). Neither the distance in space between the termination codon and the poly(A) tail nor the binding of steady-state, largely hypophosphorylated UPF1 is a discriminating marker of cellular NMD targets, unlike for premature termination codon (PTC)-containing reporter mRNAs when compared with their PTC-free counterparts...
September 1, 2014: Genes & Development
https://www.readbyqxmd.com/read/25099951/juggling-key-players-in-nmd-initiation
#15
COMMENT
Fulvia Bono
In this issue of Structure, Melero and colleagues use electron microscopy combined with biochemistry to provide structural insight into the complex between SMG1, SMG8, SMG9, UPF1, and UPF2, elucidating how key players in nonsense-mediated mRNA decay assemble at the initial steps of the process.
August 5, 2014: Structure
https://www.readbyqxmd.com/read/25053839/a-novel-phosphorylation-independent-interaction-between-smg6-and-upf1-is-essential-for-human-nmd
#16
Pamela Nicholson, Christoph Josi, Hitomi Kurosawa, Akio Yamashita, Oliver Mühlemann
Eukaryotic mRNAs with premature translation-termination codons (PTCs) are recognized and eliminated by nonsense-mediated mRNA decay (NMD). NMD substrates can be degraded by different routes that all require phosphorylated UPF1 (P-UPF1) as a starting point. The endonuclease SMG6, which cleaves mRNA near the PTC, is one of the three known NMD factors thought to be recruited to nonsense mRNAs via an interaction with P-UPF1, leading to eventual mRNA degradation. By artificial tethering of SMG6 and mutants thereof to a reporter mRNA combined with knockdowns of various NMD factors, we demonstrate that besides its endonucleolytic activity, SMG6 also requires UPF1 and SMG1 to reduce reporter mRNA levels...
August 2014: Nucleic Acids Research
https://www.readbyqxmd.com/read/25013172/phospho-dependent-and-phospho-independent-interactions-of-the-helicase-upf1-with-the-nmd-factors-smg5-smg7-and-smg6
#17
Sutapa Chakrabarti, Fabien Bonneau, Steffen Schüssler, Elfriede Eppinger, Elena Conti
Nonsense-mediated mRNA decay (NMD) is a eukaryotic surveillance pathway that recognizes mRNAs with premature stop codons and targets them for rapid degradation. Evidence from previous studies has converged on UPF1 as the central NMD factor. In human cells, the SMG1 kinase phosphorylates UPF1 at the N-terminal and C-terminal tails, in turn allowing the recruitment of the NMD factors SMG5, SMG6 and SMG7. To understand the molecular mechanisms, we recapitulated these steps of NMD in vitro using purified components...
August 2014: Nucleic Acids Research
https://www.readbyqxmd.com/read/25002321/structures-of-smg1-upfs-complexes-smg1-contributes-to-regulate-upf2-dependent-activation-of-upf1-in-nmd
#18
Roberto Melero, Akiko Uchiyama, Raquel Castaño, Naoyuki Kataoka, Hitomi Kurosawa, Shigeo Ohno, Akio Yamashita, Oscar Llorca
SMG1, a PI3K-related kinase, plays a critical role in nonsense-mediated mRNA decay (NMD) in mammals. SMG1-mediated phosphorylation of the UPF1 helicase is an essential step during NMD initiation. Both SMG1 and UPF1 are presumably activated by UPF2, but this regulation is incompletely understood. Here we reveal that SMG1C (a complex containing SMG1, SMG8, and SMG9) contributes to regulate NMD by recruiting UPF1 and UPF2 to distinct sites in the vicinity of the kinase domain. UPF2 binds SMG1 in an UPF1-independent manner in vivo, and the SMG1C-UPF2 structure shows UPF2 recognizes the FRB domain, a region that regulates the related mTOR kinase...
August 5, 2014: Structure
https://www.readbyqxmd.com/read/24947499/nonsense-mediated-decay-factors-are-involved-in-the-regulation-of-selenoprotein-mrna-levels-during-selenium-deficiency
#19
Ali Seyedali, Marla J Berry
Selenoproteins contain the unique amino acid selenocysteine (Sec), which is encoded by the triplet UGA. Since UGA also serves as a stop codon, it has been postulated that selenoprotein mRNAs are targeted for degradation by the nonsense-mediated mRNA decay pathway (NMD). Several reports have observed a hierarchy of selenoprotein mRNA expression when selenium (Se) is limiting, whereby the abundance of certain transcripts decline while others do not. We sought to investigate the role of NMD in this hierarchical response that selenoprotein mRNAs exhibit to environmental Se status...
August 2014: RNA
https://www.readbyqxmd.com/read/24927145/biochemical-properties-of-a-new-cold-active-mono-and-diacylglycerol-lipase-from-marine-member-janibacter-sp-strain-htcc2649
#20
Dongjuan Yuan, Dongming Lan, Ruipu Xin, Bo Yang, Yonghua Wang
Mono- and di-acylglycerol lipase has been applied to industrial usage in oil modification for its special substrate selectivity. Until now, the reported mono- and di-acylglycerol lipases from microorganism are limited, and there is no report on the mono- and di-acylglycerol lipase from bacteria. A predicted lipase (named MAJ1) from marine Janibacter sp. strain HTCC2649 was purified and biochemical characterized. MAJ1 was clustered in the family I.7 of esterase/lipase. The optimum activity of the purified MAJ1 occurred at pH 7...
2014: International Journal of Molecular Sciences
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