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https://www.readbyqxmd.com/read/29596649/the-loss-of-smg1-causes-defects-in-quality-control-pathways-in-physcomitrella-patens
#1
James P B Lloyd, Daniel Lang, Andreas D Zimmer, Barry Causier, Ralf Reski, Brendan Davies
Nonsense-mediated mRNA decay (NMD) is important for RNA quality control and gene regulation in eukaryotes. NMD targets aberrant transcripts for decay and also directly influences the abundance of non-aberrant transcripts. In animals, the SMG1 kinase plays an essential role in NMD by phosphorylating the core NMD factor UPF1. Despite SMG1 being ubiquitous throughout the plant kingdom, little is known about its function, probably because SMG1 is atypically absent from the genome of the model plant, Arabidopsis thaliana...
March 27, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29514868/identifying-rna-splicing-factors-using-ift-genes-in-chlamydomonas-reinhardtii
#2
Huawen Lin, Zhengyan Zhang, Carlo Iomini, Susan K Dutcher
Intraflagellar transport moves proteins in and out of flagella/cilia and it is essential for the assembly of these organelles. Using whole-genome sequencing, we identified splice site mutations in two IFT genes, IFT81 ( fla9 ) and IFT121 ( ift121-2 ), which lead to flagellar assembly defects in the unicellular green alga Chlamydomonas reinhardtii The splicing defects in these ift mutants are partially corrected by mutations in two conserved spliceosome proteins, DGR14 and FRA10. We identified a dgr14 deletion mutant, which suppresses the 3' splice site mutation in IFT81 , and a frameshift mutant of FRA10 , which suppresses the 5' splice site mutation in IFT121 Surprisingly, we found dgr14-1 and fra10 mutations suppress both splice site mutations...
March 2018: Open Biology
https://www.readbyqxmd.com/read/29470645/branching-the-tel2-pathway-for-exact-fit-on-phosphatidylinositol-3-kinase-related-kinases
#3
REVIEW
Katsunori Sugimoto
Phosphatidylinositol 3-kinase-related kinases (PIKKs), are structurally related to phosphatidylinositol 3-kinase (lipid kinase), but possess protein kinase activities. PIKKs include ATM, ATR, DNA-PK, mTOR and SMG1, key regulators of cell proliferation and genome maintenance. TRRAP, which is devoid of protein kinase activity, is the sixth member of the PIKK family. PIKK family members are gigantic proteins in the range of 300-500 kDa. It has become apparent in the last decade that the stability or maturation of the PIKK family members depends on a molecular chaperone called the Tel2-Tti1-Tti2 (TTT) complex...
February 22, 2018: Current Genetics
https://www.readbyqxmd.com/read/29192227/conservation-of-nonsense-mediated-mrna-decay-complex-components-throughout-eukaryotic-evolution
#4
Barry Causier, Zhen Li, Riet De Smet, James P B Lloyd, Yves Van de Peer, Brendan Davies
Nonsense-mediated mRNA decay (NMD) is an essential eukaryotic process regulating transcript quality and abundance, and is involved in diverse processes including brain development and plant defenses. Although some of the NMD machinery is conserved between kingdoms, little is known about its evolution. Phosphorylation of the core NMD component UPF1 is critical for NMD and is regulated in mammals by the SURF complex (UPF1, SMG1 kinase, SMG8, SMG9 and eukaryotic release factors). However, since SMG1 is reportedly missing from the genomes of fungi and the plant Arabidopsis thaliana, it remains unclear how UPF1 is activated outside the metazoa...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29164236/posttranscriptional-regulation-of-loxl1-expression-via-alternative-splicing-and-nonsense-mediated-mrna-decay-as-an-adaptive-stress-response
#5
Daniel Berner, Matthias Zenkel, Francesca Pasutto, Ursula Hoja, Panah Liravi, Gabriele C Gusek-Schneider, Friedrich E Kruse, Johannes Schödel, Andre Reis, Ursula Schlötzer-Schrehardt
Purpose: Alternative mRNA splicing coupled to nonsense-mediated decay (NMD) is a common mRNA surveillance pathway also known to dynamically modulate gene expression in response to cellular stress. Here, we investigated the involvement of this pathway in the regulation of lysyl oxidase-like 1 (LOXL1) expression in response to pseudoexfoliation (PEX)-associated pathophysiologic factors. Methods: Transcript levels of LOXL1 isoforms were determined in ocular tissues obtained from donor eyes without and with PEX syndrome...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28948493/highly-efficient-deacidification-of-high-acid-rice-bran-oil-using-methanol-as-a-novel-acyl-acceptor
#6
Daoming Li, Muniba Faiza, Shahid Ali, Weifei Wang, Chin Ping Tan, Bo Yang, Yonghua Wang
A highly efficient process for reducing the fatty acid (FA) content of high-acid rice bran oil (RBO) was developed by immobilized partial glycerides-selective lipase SMG1-F278N-catalyzed esterification/transesterification using methanol as a novel acyl acceptor. Molecular docking simulation indicated that methanol was much closer to the catalytic serine (Ser-171) compared with ethanol and glycerol, which might be one of the reasons for its high efficiency in the deacidification of high-acid RBO. Additionally, the reaction parameters were optimized to minimize the FA content of high-acid RBO...
September 25, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28389433/structure-of-a-smg8-smg9-complex-identifies-a-g-domain-heterodimer-in-the-nmd-effector-proteins
#7
Liang Li, Mahesh Lingaraju, Claire Basquin, Jérome Basquin, Elena Conti
Nonsense-mediated mRNA decay (NMD) is a eukaryotic mRNA degradation pathway involved in surveillance and post-transcriptional regulation, and executed by the concerted action of several trans -acting factors. The SMG1 kinase is an essential NMD factor in metazoans and is associated with two recently identified and yet poorly characterized proteins, SMG8 and SMG9. We determined the 2.5 Å resolution crystal structure of a SMG8-SMG9 core complex from C. elegans We found that SMG8-SMG9 is a G-domain heterodimer with architectural similarities to the dynamin-like family of GTPases such as Atlastin and GBP1...
July 2017: RNA
https://www.readbyqxmd.com/read/28107519/cudraflavone-c-induces-tumor-specific-apoptosis-in-colorectal-cancer-cells-through-inhibition-of-the-phosphoinositide-3-kinase-pi3k-akt-pathway
#8
Hsien-Chuen Soo, Felicia Fei-Lei Chung, Kuan-Hon Lim, Veronica Alicia Yap, Tracey D Bradshaw, Ling-Wei Hii, Si-Hoey Tan, Sze-Jia See, Yuen-Fen Tan, Chee-Onn Leong, Chun-Wai Mai
Cudraflavone C (Cud C) is a naturally-occurring flavonol with reported anti-proliferative activities. However, the mechanisms by which Cud C induced cytotoxicity have yet to be fully elucidated. Here, we investigated the effects of Cud C on cell proliferation, caspase activation andapoptosis induction in colorectal cancer cells (CRC). We show that Cud C inhibits cell proliferation in KM12, Caco-2, HT29, HCC2998, HCT116 and SW48 CRC but not in the non-transformed colorectal epithelial cells, CCD CoN 841. Cud C induces tumor-selective apoptosis via mitochondrial depolarization and activation of the intrinsic caspase pathway...
2017: PloS One
https://www.readbyqxmd.com/read/28054942/a-comprehensive-proteomic-survey-of-aba-induced-protein-phosphorylation-in-rice-oryza-sativa-l
#9
Jiehua Qiu, Yuxuan Hou, Yifeng Wang, Zhiyong Li, Juan Zhao, Xiaohong Tong, Haiyan Lin, Xiangjin Wei, Hejun Ao, Jian Zhang
abscisic acid (ABA) is a key phytohormone regulating plant development and stress response. The signal transduction of ABA largely relies on protein phosphorylation. However; little is known about the phosphorylation events occurring during ABA signaling in rice thus far. By employing a label-free; MS (Mass Spectrometry)-based phosphoproteomic approach; we identified 2271 phosphosites of young rice seedlings and their intensity dynamics in response to ABA; during which 1060 proteins were found to be differentially phosphorylated...
January 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27773123/the-role-of-smg1-expression-in-b-cell-lymphoproliferative-disease
#10
Patricia Rebeiro, Alexander James, Silvia Ling, Nicole Caxeiro, C Soon Lee, Tara Roberts
No abstract text is available yet for this article.
February 2016: Pathology
https://www.readbyqxmd.com/read/27571030/simplified-enzymatic-upgrading-of-high-acid-rice-bran-oil-using-ethanol-as-a-novel-acyl-acceptor
#11
Daoming Li, Weifei Wang, Rabia Durrani, Xingxing Li, Bo Yang, Yonghua Wang
One of the major challenges in the upgrading of high-acid rice bran oil (RBO) is to efficiently reduce the amount of free fatty acids. Here we report a novel method for upgrading high-acid RBO using ethanol as a novel acyl acceptor in combination with a highly selective lipase from Malassezia globosa (SMG1-F278N). This process enabled an unprecedented deacidification efficiency of up to 99.80% in a short time (6 h); the immobilized SMG1-F278N used in deacidification exhibited excellent operational stability and could be used for at least 10 consecutive batches without detectable loss in activity...
September 7, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27540752/a-novel-process-for-the-synthesis-of-highly-pure-n-3-polyunsaturated-fatty-acid-pufa-enriched-triglycerides-by-combined-transesterification-and-ethanolysis
#12
Daoming Li, Weifei Wang, Xiaoli Qin, Xingxing Li, Bo Yang, Yonghua Wang
In this study, a novel two-step enzymatic reaction was developed for the synthesis of highly pure triacylglycerols (TAGs) with a high content of n-3 polyunsaturated fatty acids (PUFAs). Glyceride mixtures were primarily synthesized by Novozym 435-catalyzed transesterification of glycerol and DHA/EPA-rich ethyl esters (EEs), followed by removal of partial glycerides, for the first time, by immobilized mono- and diacylglycerol lipase SMG1-F278N-catalyzed ethanolysis. TAG yield as high as 98.66% was achieved under the optimized conditions, and highly pure (98...
August 31, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27419053/lid-mobility-in-lipase-smg1-validated-using-a-thiol-disulfide-redox-potential-probe
#13
Shaohua Guo, Grzegorz Maria Popowicz, Daoming Li, Dongjuan Yuan, Yonghua Wang
Most lipases possess a lid domain above the catalytic site that is responsible for their activation. Lipase SMG1 from Malassezia globose CBS 7966 (Malassezia globosa LIP1), is a mono- and diacylglycerol lipase with an atypical loop-like lid domain. Activation of SMG1 was proposed to be solely through a gating mechanism involving two residues (F278 and N102). However, through disulfide bond cross-linking of the lid, this study shows that full activation also requires mobility of the lid domain, contrary to a previous proposal...
May 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/26841701/the-rna-helicase-dhx34-functions-as-a-scaffold-for-smg1-mediated-upf1-phosphorylation
#14
Roberto Melero, Nele Hug, Andrés López-Perrote, Akio Yamashita, Javier F Cáceres, Oscar Llorca
Nonsense-mediated decay (NMD) is a messenger RNA quality-control pathway triggered by SMG1-mediated phosphorylation of the NMD factor UPF1. In recent times, the RNA helicase DHX34 was found to promote mRNP remodelling, leading to activation of NMD. Here we demonstrate the mechanism by which DHX34 functions in concert with SMG1. DHX34 comprises two distinct structural units, a core that binds UPF1 and a protruding carboxy-terminal domain (CTD) that binds the SMG1 kinase, as shown using truncated forms of DHX34 and electron microscopy of the SMG1-DHX34 complex...
February 4, 2016: Nature Communications
https://www.readbyqxmd.com/read/26365206/structure-of-product-bound-smg1-lipase-active-site-gating-implications
#15
Shaohua Guo, Jinxin Xu, Ioannis V Pavlidis, Dongming Lan, Uwe T Bornscheuer, Jinsong Liu, Yonghua Wang
Monoacylglycerol and diacylglycerol lipases are industrially interesting enzymes, due to the health benefits that arise from the consumption of diglycerides compared to the traditional triglyceride oils. Most lipases possess an α-helix (lid) directly over the catalytic pocket which regulates the activity of the enzyme. Generally, lipases exist in active and inactive conformations, depending on the positioning of this lid subdomain. However, lipase SMG1, a monoacylglycerol and diacylglycerol specific lipase, has an atypical activation mechanism...
December 2015: FEBS Journal
https://www.readbyqxmd.com/read/26239010/the-role-of-residues-103-104-and-278-in-the-activity-of-smg1-lipase-from-malassezia-globosa-a-site-directed-mutagenesis-study
#16
Dongming Lan, Qian Wang, Grzegorz Maria Popowicz, Bo Yang, Qingyun Tang, Yonghua Wang
The SMG1 lipase from Malassezia globosa is a newly found mono- and diacylglycerol (DAG) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. In the present study, we characterized the contribution of three residues, L103 and F104 in the lid and F278 in the rim of the binding site groove, on the function of SMG1 lipase. Sitedirected mutagenesis was conducted at these sites, and each of the mutants was expressed in the yeast Pichia pastoris, purified, and characterized for their activity toward DAG and pnitrophenol (pNP) ester...
November 2015: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/26199121/novel-inhibitor-against-malassezia-globosa-lip1-smg1-a-potential-anti-dandruff-target
#17
Shaohua Guo, Wenkang Huang, Jian Zhang, Yonghua Wang
Compelling evidence have demonstrated the role of lipase activity in the pathogenicity of Malassezia globosa toward dandruff and seborrheic dermatitis (D/SD). As a representative secreted lipase from M. globosa CBS 7966, Malassezia globosa LIP1 (SMG1) is considered a potential anti-dandruff target. In this study, homology modeling, docking-based virtual screening and in vitro lipase-based assay were integrated to identify the first hit compound against SMG1, with an IC50 of 20 μM against synthetic lipase substrate, and of 0...
September 1, 2015: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/25837472/residue-asn277-affects-the-stability-and-substrate-specificity-of-the-smg1-lipase-from-malassezia-globosa
#18
Dongming Lan, Qian Wang, Jinxin Xu, Pengfei Zhou, Bo Yang, Yonghua Wang
Thermostability and substrate specificity are important characteristics of enzymes for industrial application, which can be improved by protein engineering. SMG1 lipase from Malassezia globosa is a mono- and diacylglycerol lipase (MDL) that shows activity toward mono- and diacylglycerols, but no activity toward triacylglycerols. SMG1 lipase is considered a potential biocatalyst applied in oil/fat modification and its crystal structure revealed that an interesting residue-Asn277 may contribute to stabilize loop 273-278 and the 3104 helix which are important to enzyme characterization...
2015: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/25783054/a-mechanistic-study-into-the-epoxidation-of-carboxylic-acid-and-alkene-in-a-mono-di-acylglycerol-lipase
#19
Xuping Wang, Qingyun Tang, Grzegorz Maria Popowicz, Bo Yang, Yonghua Wang
More and more industrial chemistry reactions rely on green technologies. Enzymes are finding increasing use in diverse chemical processes. Epoxidized vegetable oils have recently found applications as plasticizers and additives for PVC production. We report here an unusual activity of the Malassezia globosa lipase (SMG1) that is able to catalyze epoxidation of alkenes. SMG1 catalyzes formation of peroxides from long chain carboxylic acids that subsequently react with double bonds of alkenes to produce epoxides...
May 1, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/25520033/biological-and-clinical-significance-of-epigenetic-silencing-of-marveld1-gene-in-lung-cancer
#20
Ming Shi, Shan Wang, Yuanfei Yao, Yiqun Li, Hao Zhang, Fang Han, Huan Nie, Jie Su, Zeyu Wang, Lei Yue, Jingyan Cao, Yu Li
Epigenetic silence in cancer frequently altered signal-transduction pathways during the early stages of tumor development. Recent progress in the field of cancer epigenetics has led to new opportunities for diagnosis and treatment of cancer. We previously demonstrated that novel identified nuclear factor MARVELD1 was widely expressed in human tissues, but down-regulated by promoter methylation in multiple cancers. This study was carried out to determine the biological and clinical significance of MARVELD1 gene silencing in lung cancer...
2014: Scientific Reports
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