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https://www.readbyqxmd.com/read/27708137/an-nxf1-mrna-with-a-retained-intron-is-expressed-in-hippocampal-and-neocortical-neurons-and-is-translated-into-a-protein-that-functions-as-an-nxf1-cofactor
#1
Ying Li, Yeou-Cherng Bor, Mark P Fitzgerald, Kevin S Lee, David Rekosh, Marie-Louise Hammarskjold
The Nxf1 protein is a major nuclear export receptor for the transport of mRNA, and it also is essential for export of retroviral mRNAs with retained introns. In the latter case, it binds to RNA elements known as constitutive transport elements (CTEs) and functions in conjunction with a cofactor known as Nxt1. The NXF1 gene also regulates expression of its own intron-containing RNA through the use of a functional CTE within intron 10. mRNA containing this intron is exported to the cytoplasm, where it can be translated into the 356-amino acid short Nxf1(sNxf1) protein, despite the fact that it is a prime candidate for nonsense-mediated decay (NMD)...
December 1, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27433972/rna-binding-protein-stau2-is-important-for-spindle-integrity-and-meiosis-progression-in-mouse-oocytes
#2
Yan Cao, Juan Du, Dandan Chen, Qian Wang, Nana Zhang, Xiaoyun Liu, Xiaoyu Liu, Jing Weng, Yuanjing Liang, Wei Ma
Staufen2 (Stau2) is a double-stranded RNA-binding protein involved in cell fate decision by regulating mRNA transport, mRNA stability, translation, and ribonucleoprotein assembly. Little is known about Stau2 expression and function in mammalian oocytes during meiosis. Herein we report the sub-cellular distribution and function of Stau2 in mouse oocyte meiosis. Western blot analysis revealed high and stable expression of Stau2 in oocytes from germinal vesicle (GV) to metaphase II (MII). Immunofluorescence showed that Stau2 was evenly distributed in oocytes at GV stage, and assembled as filaments after germinal vesicle breakdown (GVBD), particularly, colocalized with spindle at MI and MII...
October 2016: Cell Cycle
https://www.readbyqxmd.com/read/26843428/the-downregulation-of-the-rna-binding-protein-staufen2-in-response-to-dna-damage-promotes-apoptosis
#3
Xin Zhang, Véronique Trépanier, Remy Beaujois, Wildriss Viranaicken, Elliot Drobetsky, Luc DesGroseillers
Staufen2 (Stau2) is an RNA-binding protein involved in cell fate decision by controlling several facets of mRNA processing including localization, splicing, translation and stability. Herein we report that exposure to DNA-damaging agents that generate replicative stress such as camptothecin (CPT), 5-fluoro-uracil (5FU) and ultraviolet radiation (UVC) causes downregulation of Stau2 in HCT116 colorectal cancer cells. In contrast, other agents such as doxorubicin and ionizing radiation had no effect on Stau2 expression...
May 5, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/24360961/staufen2-regulates-neuronal-target-rnas
#4
Jacki E Heraud-Farlow, Tejaswini Sharangdhar, Xiao Li, Philipp Pfeifer, Stefanie Tauber, Denise Orozco, Alexandra Hörmann, Sabine Thomas, Anetta Bakosova, Ashley R Farlow, Dieter Edbauer, Howard D Lipshitz, Quaid D Morris, Martin Bilban, Michael Doyle, Michael A Kiebler
RNA-binding proteins play crucial roles in directing RNA translation to neuronal synapses. Staufen2 (Stau2) has been implicated in both dendritic RNA localization and synaptic plasticity in mammalian neurons. Here, we report the identification of functionally relevant Stau2 target mRNAs in neurons. The majority of Stau2-copurifying mRNAs expressed in the hippocampus are present in neuronal processes, further implicating Stau2 in dendritic mRNA regulation. Stau2 targets are enriched for secondary structures similar to those identified in the 3' UTRs of Drosophila Staufen targets...
December 26, 2013: Cell Reports
https://www.readbyqxmd.com/read/24360960/interactome-of-two-diverse-rna-granules-links-mrna-localization-to-translational-repression-in-neurons
#5
Renate Fritzsche, Daniela Karra, Keiryn L Bennett, Foong Yee Ang, Jacki E Heraud-Farlow, Marco Tolino, Michael Doyle, Karl E Bauer, Sabine Thomas, Melanie Planyavsky, Eric Arn, Anetta Bakosova, Kerstin Jungwirth, Alexandra Hörmann, Zsofia Palfi, Julia Sandholzer, Martina Schwarz, Paolo Macchi, Jacques Colinge, Giulio Superti-Furga, Michael A Kiebler
Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a stringent protocol to biochemically purify RNA granules from rat brain. Here, we identified a specific set of interactors for two RNA-binding proteins that are known components of neuronal RNA granules, Barentsz and Staufen2...
December 26, 2013: Cell Reports
https://www.readbyqxmd.com/read/23263869/staufen2-functions-in-staufen1-mediated-mrna-decay-by-binding-to-itself-and-its-paralog-and-promoting-upf1-helicase-but-not-atpase-activity
#6
Eonyoung Park, Michael L Gleghorn, Lynne E Maquat
Staufen (STAU)1-mediated mRNA decay (SMD) is a posttranscriptional regulatory mechanism in mammals that degrades mRNAs harboring a STAU1-binding site (SBS) in their 3'-untranslated regions (3' UTRs). We show that SMD involves not only STAU1 but also its paralog STAU2. STAU2, like STAU1, is a double-stranded RNA-binding protein that interacts directly with the ATP-dependent RNA helicase up-frameshift 1 (UPF1) to reduce the half-life of SMD targets that form an SBS by either intramolecular or intermolecular base-pairing...
January 8, 2013: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/22902295/asymmetric-segregation-of-the-double-stranded-rna-binding-protein-staufen2-during-mammalian-neural-stem-cell-divisions-promotes-lineage-progression
#7
Gretchen Kusek, Melissa Campbell, Frank Doyle, Scott A Tenenbaum, Michael Kiebler, Sally Temple
Asymmetric cell divisions are a fundamental feature of neural development, and misregulation can lead to brain abnormalities or tumor formation. During an asymmetric cell division, molecular determinants are segregated preferentially into one daughter cell to specify its fate. An important goal is to identify the asymmetric determinants in neural progenitor cells, which could be tumor suppressors or inducers of specific neural fates. Here, we show that the double-stranded RNA-binding protein Stau2 is distributed asymmetrically during progenitor divisions in the developing mouse cortex, preferentially segregating into the Tbr2(+) neuroblast daughter, taking with it a subset of RNAs...
October 5, 2012: Cell Stem Cell
https://www.readbyqxmd.com/read/22902294/an-asymmetrically-localized-staufen2-dependent-rna-complex-regulates-maintenance-of-mammalian-neural-stem-cells
#8
John P Vessey, Gianluca Amadei, Sarah E Burns, Michael A Kiebler, David R Kaplan, Freda D Miller
The cellular mechanisms that regulate self-renewal versus differentiation of mammalian somatic tissue stem cells are still largely unknown. Here, we asked whether an RNA complex regulates this process in mammalian neural stem cells. We show that the RNA-binding protein Staufen2 (Stau2) is apically localized in radial glial precursors of the embryonic cortex, where it forms a complex with other RNA granule proteins including Pumilio2 (Pum2) and DDX1, and the mRNAs for β-actin and mammalian prospero, prox1. Perturbation of this complex by functional knockdown of Stau2, Pum2, or DDX1 causes premature differentiation of radial glial precursors into neurons and mislocalization and misexpression of prox1 mRNA...
October 5, 2012: Cell Stem Cell
https://www.readbyqxmd.com/read/22162130/xenopus-staufen2-is-required-for-anterior-endodermal-organ-formation
#9
Cassandra K Bilogan, Marko E Horb
Defining the regulatory molecular networks involved in patterning the developing anterior endoderm is essential to understand how the pancreas, liver, stomach, and duodenum are discretely specified from each other. In this study, we analyzed the expression and function of the double-stranded RNA-binding protein Staufen2 in Xenopus laevis endoderm. We found that staufen2 was broadly expressed within the developing endoderm beginning at gastrulation becoming localized to the anterior endoderm at later stages...
March 2012: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/22087843/cell-type-dependent-gene-regulation-by-staufen2-in-conjunction-with-upf1
#10
Takashi Miki, Yasunao Kamikawa, Sadamu Kurono, Yuka Kaneko, Jun Katahira, Yoshihiro Yoneda
UNLABELLED: dendritic mRNA transport machines. Although Stau2 is thought to be involved in the dendritic targeting of several mRNAs in neurons, the mechanism whereby Stau2 regulates these mRNAs is unknown. To elucidate the functions of Stau2, we screened for novel binding partners by affinity purification of GST-tagged Stau2 from 293F cells. RESULTS: Three RNA helicases, RNA helicase A, Upf1 and Mov10, were identified in Stau2-containing complexes. We focused our studies on Upf1, a key player in nonsense-mediated mRNA decay...
2011: BMC Molecular Biology
https://www.readbyqxmd.com/read/20596529/molecular-composition-of-staufen2-containing-ribonucleoproteins-in-embryonic-rat-brain
#11
Marjolaine Maher-Laporte, Frédéric Berthiaume, Mireille Moreau, Louis-André Julien, Gabriel Lapointe, Michael Mourez, Luc DesGroseillers
Messenger ribonucleoprotein particles (mRNPs) are used to transport mRNAs along neuronal dendrites to their site of translation. Numerous mRNA-binding and regulatory proteins within mRNPs finely regulate the fate of bound-mRNAs. Their specific combination defines different types of mRNPs that in turn are related to specific synaptic functions. One of these mRNA-binding proteins, Staufen2 (Stau2), was shown to transport dendritic mRNAs along microtubules. Its knockdown expression in neurons was shown to change spine morphology and synaptic functions...
June 28, 2010: PloS One
https://www.readbyqxmd.com/read/20510018/genome-wide-identification-of-staufen2-bound-mrnas-in-embryonic-rat-brains
#12
Marjolaine Maher-Laporte, Luc DesGroseillers
Messenger ribonucleoprotein particles (mRNPs) are used to transport mRNAs along neuronal dendrites to their site of translation. Staufen2 is an mRNA-binding protein expressed in the cell bodies and cellular processes of different brain cells. It is notably involved in the transport of dendritic mRNAs along microtubules. Its knockdown expression was shown to change spine morphology and impair synaptic functions. However, the identity of Staufen2-bound mRNAs in brain cells is still completely unknown. As a mean to identify these mRNAs, we immunoprecipitated Staufen2-containing mRNPs from embryonic rat brains and used a genome wide approach to identify Staufen2-associated mRNAs...
May 2010: BMB Reports
https://www.readbyqxmd.com/read/20456011/apolipoprotein-e-mrna-is-transported-to-dendrites-and-may-have-a-role-in-synaptic-structural-plasticity
#13
Jun-Young Oh, Yeon-Ju Nam, Anna Jo, Hyo-Soon Cheon, Sang-Myung Rhee, Joong-Ki Park, Jin-A Lee, Hyong Kyu Kim
Although the dendritic localization and translation of a subset of mRNAs plays a pivotal role in synaptic plasticity, the dendritic mRNAs and their functions have been only minimally characterized thus far. In this study, we isolated mRNAs from Staufen2-containing ribonucleoprotein complexes, which function as modules for the transport of mRNA to the dendrites, and then constructed a cDNA library. Apolipoprotein E gene (APOE) mRNA was isolated from the dendritic mRNA-specific cDNA library. The specific localization of APOE mRNA was evaluated via in situ hybridization...
August 2010: Journal of Neurochemistry
https://www.readbyqxmd.com/read/18492489/role-of-mitogen-activated-protein-kinase-mapk-docking-sites-on-staufen2-protein-in-dendritic-mrna-transport
#14
Yeon-Ju Nam, Hyo-Soon Cheon, Young-Ki Choi, Seok-Yong Kim, Eun-Young Shin, Eung-Gook Kim, Hyong Kyu Kim
Although transport and subsequent translation of dendritic mRNA play an important role in neuronal synaptic plasticity, the underlying mechanisms for modulating dendritic mRNA transport are almost completely unknown. In this study, we identified and characterized an interaction between Staufen2 and mitogen-activated protein kinase (MAPK) with co-immunoprecipitation assays. Staufen2 utilized a docking (D) site to interact with ERK1/2; deleting the D-site decreased colocalization of Staufen2 with immunoreactive ERK1/2 in the cell body regions of cultured hippocampal neurons, and it reduced the amount of Staufen2-containing RNP complexes in the distal dendrites...
August 8, 2008: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/18094122/a-genome-wide-approach-identifies-distinct-but-overlapping-subsets-of-cellular-mrnas-associated-with-staufen1-and-staufen2-containing-ribonucleoprotein-complexes
#15
Luc Furic, Marjolaine Maher-Laporte, Luc DesGroseillers
Messenger RNAs are associated with multiple RNA-binding proteins to form ribonucleoprotein (mRNP) complexes. These proteins are important regulators of the fate of their target mRNAs. In human cells, Staufen1 and Staufen2 proteins, coded by two different genes, are double-stranded RNA-binding proteins involved in several cellular functions including mRNA localization, translation, and decay. Although 51% identical, these proteins are nevertheless found in different RNA particles. In addition, differential splicing events generate Staufen2 isoforms that only differ at their N-terminal extremities...
February 2008: RNA
https://www.readbyqxmd.com/read/17587311/the-transport-of-staufen2-containing-ribonucleoprotein-complexes-involves-kinesin-motor-protein-and-is-modulated-by-mitogen-activated-protein-kinase-pathway
#16
Ji-Hye Jeong, Yeon-Ju Nam, Seok-Yong Kim, Eung-Gook Kim, Jooyoung Jeong, Hyong Kyu Kim
There is increasing evidence showing that mRNA is transported to the neuronal dendrites in ribonucleoprotein (RNP) complexes or RNA granules, which are aggregates of mRNA, rRNA, ribosomal proteins, and RNA-binding proteins. In these RNP complexes, Staufen, a double-stranded RNA-binding protein, is believed to be a core component that plays a key role in the dendritic mRNA transport. This study investigated the molecular mechanisms of the dendritic mRNA transport using green fluorescent protein-tagged Staufen2 produced employing a Sindbis viral expression system...
September 2007: Journal of Neurochemistry
https://www.readbyqxmd.com/read/16513105/zebrafish-staufen1-and-staufen2-are-required-for-the-survival-and-migration-of-primordial-germ-cells
#17
Srinivas Ramasamy, Hui Wang, Helen Ngoc Bao Quach, Karuna Sampath
In sexually reproducing organisms, primordial germ cells (PGCs) give rise to the cells of the germ line, the gametes. In many animals, PGCs are set apart from somatic cells early during embryogenesis. Work in Drosophila, C. elegans, Xenopus, and zebrafish has shown that maternally provided localized cytoplasmic determinants specify the germ line in these organisms (Raz, E., 2003. Primordial germ-cell development: the zebrafish perspective. Nat. Rev., Genet. 4, 690--700; Santos, A.C., Lehmann, R., 2004. Germ cell specification and migration in Drosophila and beyond...
April 15, 2006: Developmental Biology
https://www.readbyqxmd.com/read/16418534/the-brain-specific-double-stranded-rna-binding-protein-staufen2-is-required-for-dendritic-spine-morphogenesis
#18
Bernhard Goetze, Fabian Tuebing, Yunli Xie, Mario M Dorostkar, Sabine Thomas, Ulrich Pehl, Stefan Boehm, Paolo Macchi, Michael A Kiebler
Mammalian Staufen2 (Stau2) is a member of the double-stranded RNA-binding protein family. Its expression is largely restricted to the brain. It is thought to play a role in the delivery of RNA to dendrites of polarized neurons. To investigate the function of Stau2 in mature neurons, we interfered with Stau2 expression by RNA interference (RNAi). Mature neurons lacking Stau2 displayed a significant reduction in the number of dendritic spines and an increase in filopodia-like structures. The number of PSD95-positive synapses and miniature excitatory postsynaptic currents were markedly reduced in Stau2 down-regulated neurons...
January 16, 2006: Journal of Cell Biology
https://www.readbyqxmd.com/read/16377940/the-role-of-mammalian-staufen-on-mrna-traffic-a-view-from-its-nucleocytoplasmic-shuttling-function
#19
REVIEW
Takashi Miki, Keizo Takano, Yoshihiro Yoneda
The localization of mRNA in neuronal dendrites plays a role in both locally and temporally regulated protein synthesis, which is required for certain forms of synaptic plasticity. RNA granules constitute a dendritic mRNA transport machinery in neurons, which move along microtubules. RNA granules contain densely packed clusters of ribosomes, but lack some factors that are required for translation, suggesting that they are translationally incompetent. Recently some of the components of RNA granules have been identified, and their functions are in the process of being examined, in attempts to better understand the properties of RNA granules...
2005: Cell Structure and Function
https://www.readbyqxmd.com/read/16277607/the-zinc-finger-protein-zfr-is-critical-for-staufen-2-isoform-specific-nucleocytoplasmic-shuttling-in-neurons
#20
George Elvira, Bernard Massie, Luc DesGroseillers
In mammalian neurons, transport and translation of mRNA to individual potentiated synapses is believed to occur via a heterogeneous population of RNA granules. To identify components of Staufen2-containing granules, we used the yeast two-hybrid system. A mouse fetal cDNA library was screened with the N-terminal fragment of Staufen2 as bait. ZFR, a three zinc finger protein, was identified as an interacting protein. Confocal microscopy showed that ZFR, although mainly nuclear, was also found in the somatodendritic compartment of primary hippocampal neurons where it localized as granule-like structures...
January 2006: Journal of Neurochemistry
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