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https://www.readbyqxmd.com/read/28546386/assessment-of-gene-flow-between-gossypium-hirsutum-and-gossypium-herbaceum-evidence-of-unreduced-gametes-in-the-diploid-progenitor
#1
E Montes, O Coriton, F Eber, V Huteau, J M Lacape, C Reinhardt, D Marais, J L Hofs, A M Chèvre, C Pannetier
In the framework of a gene flow assessment, we investigated the natural hybridization rate between Gossypium hirsutum (AADD genome) and G. herbaceum (AA genome). The latter species, a diploid progenitor of G. hirsutum, is spontaneously present in South Africa. Reciprocal crosses were performed without emasculation between G. herbaceum and G. hirsutum Neither examination of the morphological characteristics nor flow cytometry analysis of the 335 plants resulting from the G. hirsutum x G. herbaceum cross showed any hybrid features...
May 25, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28537804/evaluation-of-testing-of-acute-leukemia-samples-survey-result-from-the-college-of-american-pathologists
#2
Tracy I George, Joseph A Tworek, Nicole E Thomas, Lisa A Fatheree, Rhona J Souers, Raouf E Nakhleh, Daniel A Arber
CONTEXT: - The classification and prognosis determination in acute leukemia (AL) are complex and it is unclear what testing is being performed in practice. OBJECTIVE: - To survey physicians describing their current practice of test ordering in the diagnosis of AL. DESIGN: - In anticipation of a guideline by the College of American Pathologists (CAP) and the American Society for Hematology on laboratory testing needed for the initial workup of AL, a baseline survey was designed by an expert panel from CAP...
May 24, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/28536067/relationship-between-quantitative-and-descriptive-methods-of-studying-blood-flow-through-intrapulmonary-arteriovenous-anastomoses-during-exercise
#3
Joseph W Duke, Jonathan E Elliott, Steven S Laurie, Thomas Voelkel, Igor M Gladstone, Mathews B Fish, Andrew T Lovering
Several methods exist to study intrapulmonary arteriovenous anastomoses (IPAVA) in humans. Transthoracic saline contrast echocardiography (TTSCE), i.e., bubble scores, is minimally-invasive, but cannot be used to quantify the magnitude of blood flow through IPAVA (QIPAVA). Radiolabeled macroaggregates of albumin ((99m)Tc-MAA) have been used to quantify QIPAVA in humans, but this requires injection of radioactive particles. Previous work has shown agreement between (99m)Tc-MAA and TTSCE, but this has not been tested simultaneously in the same group of subjects...
May 20, 2017: Respiratory Physiology & Neurobiology
https://www.readbyqxmd.com/read/28530564/a-new-method-for-the-rapid-detection-of-atlantic-cod-gadus-morhua-pacific-cod-gadus-macrocephalus-alaska-pollock-gadus-chalcogrammus-and-ling-molva-molva-using-a-lateral-flow-dipstick-assay
#4
Ledicia Taboada, Ana Sánchez, Ricardo I Pérez-Martín, Carmen G Sotelo
Species-specific lateral flow dipstick (LFD) assays for the identification of Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Gadus chalcogrammus) and ling (Molva molva) in food products were developed. The method comprises a PCR system with four sets of specific primers, for each target species. This step was also devised to dual-labeling of PCR products with biotin and 6-FAM, which are then easily read on a lateral flow dipstick, upon which these products are immobilized by a fixed biotin-ligand and visualized with anti-FAM antibody-coated gold nanoparticles...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28528391/dynamism-in-the-upstream-invasion-edge-of-a-freshwater-fish-exposes-range-boundary-constraints
#5
Erika S Rubenson, Julian D Olden
Studying the dynamics of species' borders can provide insight into the mechanisms limiting or promoting range expansion in response to environmental change. In the John Day River, Oregon (USA), rising stream temperatures are facilitating the upstream expansion of invasive smallmouth bass Micropterus dolomieu. Here, where smallmouth bass occupy the upstream limit of its thermal tolerance, we explore population structure and seasonal movement patterns to elucidate the environmental conditions and individual traits that define front edge (where individuals reside year-round) and leading edge (where individuals colonize, but may not establish) limits to its upstream distribution...
May 20, 2017: Oecologia
https://www.readbyqxmd.com/read/28526686/amazon-river-dolphins-inia-geoffrensis-modify-biosonar-output-level-and-directivity-during-prey-interception-in-the-wild
#6
Michael Ladegaard, Frants Havmand Jensen, Kristian Beedholm, Vera Maria Ferreira da Silva, Peter Teglberg Madsen
Toothed whales have evolved to live in extremely different habitats and yet they all rely strongly on echolocation for finding and catching prey. Such biosonar based foraging involves distinct phases of searching for, approaching, and capturing prey, where echolocating animals gradually adjust sonar output to actively shape the flow of sensory information. Measuring those outputs in absolute levels requires hydrophone arrays centred on the biosonar beam axis, but this has never been done for wild toothed whales approaching and capturing prey...
May 19, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28523449/expression-of-coagulation-factor-xiii-subunit-a-correlates-with-outcome-in-childhood-acute-lymphoblastic-leukemia
#7
Bettina Kárai, Zsuzsanna Hevessy, Eszter Szánthó, László Csáthy, Anikó Ujfalusi, Katalin Gyurina, István Szegedi, János Kappelmayer, Csongor Kiss
Previously we identified B-cell lineage leukemic lymphoblasts as a new expression site for subunit A of blood coagulation factor XIII (FXIII-A). On the basis of FXIII-A expression, various subgroups of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) can be identified. Fifty-five children with BCP-ALL were included in the study. Bone marrow samples were obtained by aspiration and the presence of FXIII-A was detected by flow cytometry. G-banding and fluorescent in situ hybridization was performed according to standard procedures...
May 18, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28521628/cd36-positive-b-lymphoblasts-predict-poor-outcome-in-children-with-b-lymphoblastic-leukemia
#8
Joanna G Newton, John T Horan, Scott Newman, Michael R Rossi, Rhett P Ketterling, Sunita I Park
Objective We observed that pediatric patients with B lymphoblastic leukemia which expressed CD36 at diagnosis seemed to have worse outcome than patients whose blasts did not. Here, we describe the patient, disease characteristics, pathological, molecular, and genetic features and outcomes of patients with CD36+ B-LL compared to patients with CD36- B-LL. Methods We retrospectively reviewed all flow cytometry reports from September 2008 to December 2015 to identify patients diagnosed at our institution with CD36 expression on B lymphoblasts...
June 2017: Pediatric and Developmental Pathology
https://www.readbyqxmd.com/read/28518171/fish-flow-a-protocol-for-the-concurrent-detection-of-mrna-and-protein-in-single-cells-using-fluorescence-in-situ-hybridization-and-flow-cytometry
#9
Riccardo Arrigucci, Yuri Bushkin, Felix Radford, Karim Lakehal, Pooja Vir, Richard Pine, December Martin, Jeffrey Sugarman, Yanlin Zhao, George S Yap, Alfred A Lardizabal, Sanjay Tyagi, Maria Laura Gennaro
We describe a flow-cytometry-based protocol for intracellular mRNA measurements in nonadherent mammalian cells using fluorescence in situ hybridization (FISH) probes. The method, which we call FISH-Flow, allows for high-throughput multiparametric measurements of gene expression, a task that was not feasible with earlier, microscopy-based approaches. The FISH-Flow protocol involves cell fixation, permeabilization and hybridization with a set of fluorescently labeled oligonucleotide probes. In this protocol, surface and intracellular protein markers can also be stained with fluorescently labeled antibodies for simultaneous protein and mRNA measurement...
June 2017: Nature Protocols
https://www.readbyqxmd.com/read/28515781/evolutionary-restoration-potential-evaluated-through-the-use-of-a-trait-linked-genetic-marker
#10
Travis M Apgar, Devon E Pearse, Eric P Palkovacs
Human-driven evolution can impact the ecological role and conservation value of impacted populations. Most evolutionary restoration approaches focus on manipulating gene flow, but an alternative approach is to manipulate the selection regime to restore historical or desired trait values. Here we examined the potential utility of this approach to restore anadromous migratory behavior in coastal California steelhead trout (Oncorhynchus mykiss) populations. We evaluated the effects of natural and anthropogenic environmental variables on the observed frequency of alleles at a genomic marker tightly associated with migratory behavior across 39 steelhead populations from across California, USA...
June 2017: Evolutionary Applications
https://www.readbyqxmd.com/read/28515389/ca-2-in-hybridization-solutions-for-fluorescence-in-situ-hybridization-facilitates-the-detection-of-enterobacteriaceae
#11
Shin Haruta, Takao Iino, Moriya Ohkuma, Ken-Ichiro Suzuki, Yasuo Igarashi
Fluorescence in situ hybridization (FISH) has been employed to identify microorganisms at the single cell level under a microscope. Extensive efforts have been made to improve and extend the FISH technique; however, the development of a widely applicable protocol is a continuing challenge. The present study evaluated the effects of divalent cations in the hybridization solution on the FISH-based detection of various species of bacteria and archaea with rRNA-targeted probes. A flow cytometric analysis after FISH with a standard hybridization buffer detected positive signals from less than 30% of Escherichia coli IAM 1264 cells...
May 18, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28515171/mutualistic-damselfish-induce-higher-photosynthetic-rates-in-their-host-coral
#12
Nur Garcia-Herrera, Sebastian C A Ferse, Andreas Kunzmann, Amatzia Genin
Coral reefs are amongst the most diverse ecosystems on Earth where complex inter-specific interactions are ubiquitous. An example of such interactions is the mutualistic relationship between damselfishes and branching corals in the Northern Red Sea, where the fish use corals as shelter and provide them with nutrients, enhance the flow between their branches, and protect them from predators. By enhancing the flow between the coral branches, the fish ventilate the coral's inner zone, mitigating hypoxic conditions that otherwise develop within that zone during the night...
May 15, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28515149/pyruvate-kinase-inhibits-proliferation-during-postnatal-cerebellar-neurogenesis-and-suppresses-medulloblastoma-formation
#13
Katherine Tech, Andrey P Tikunov, Hamza Farooq, A Sorana Morrissy, Jessica Meidinger, Taylor Fish, Sarah C Green, Hedi Liu, Yisu Li, Andrew J Mungall, Richard A Moore, Yussanne Ma, Steven Jm Jones, Marco A Marra, Matthew G Vander Heiden, Michael D Taylor, Jeffrey MacDonald, Timothy R Gershon
Aerobic glycolysis supports proliferation through unresolved mechanisms. We have previously shown that aerobic glycolysis is required for the regulated proliferation of cerebellar granule neuron progenitors (CGNPs), and for the growth of CGNP-derived medulloblastoma. Blocking the initiation of glycolysis via deletion of Hexokinase-2 (Hk2) disrupts CGNP proliferation and restricts medulloblastoma growth. Here, we assessed whether disrupting Pyruvate kinase-M (Pkm), an enzyme that acts in the terminal steps of glycolysis, would alter CGNP metabolism, proliferation and tumorigenesis...
May 17, 2017: Cancer Research
https://www.readbyqxmd.com/read/28510256/interregional-cytogenetic-comparisons-in-halichoeres-and-thalassoma-wrasses-labridae-of-coastal-and-insular-regions-of-the-southwestern-atlantic
#14
K D J Amorim, M B Cioffi, L A C Bertollo, R X Soares, L L Calado, A T Borges, G W W F Costa, W F Molina
The distribution patterns of marine biodiversity are complex, resulting from vicariant events and species dispersion, as well as local ecological and adaptive conditions. Furthermore, the wide geographic distribution of some species may be hindered by biogeographical barriers that can interfere in the gene flow. Cytogenetic analyses in marine fishes, especially those involving populations in small remote insular environments, remain scarce. In the Western Atlantic, species of wrasses from the genera Halichoeres and Thalassoma occur in biogeographic arrangements that make it possible to analyze cytogenetic patterns between coastal and widely separated island populations...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28503173/microbial-community-response-to-simulated-petroleum-seepage-in-caspian-sea-sediments
#15
Marion H Stagars, Sonakshi Mishra, Tina Treude, Rudolf Amann, Katrin Knittel
Anaerobic microbial hydrocarbon degradation is a major biogeochemical process at marine seeps. Here we studied the response of the microbial community to petroleum seepage simulated for 190 days in a sediment core from the Caspian Sea using a sediment-oil-flow-through (SOFT) system. Untreated (without simulated petroleum seepage) and SOFT sediment microbial communities shared 43% bacterial genus-level 16S rRNA-based operational taxonomic units (OTU0.945) but shared only 23% archaeal OTU0.945. The community differed significantly between sediment layers...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28501197/methylmercury-determination-in-seafood-by-photochemical-vapor-generation-capacitively-coupled-plasma-microtorch-optical-emission-spectrometry
#16
Eniko Covaci, Marin Senila, Michaela Ponta, Eugen Darvasi, Dorin Petreus, Maria Frentiu, Tiberiu Frentiu
A non-chromatographic method based on double liquid-liquid extraction and measurements by UV photochemical vapor generation capacitively coupled plasma microtorch optical emission spectrometry was developed and characterized for methylmercury determination in seafood. Samples were prepared following the procedure recommended in JRC Technical Report of European Commission formerly proposed for the determination of methylmercury in seafood by thermal decomposition atomic absorption spectrometry, namely confinement of Hg species in 47% HBr solution, extraction of CH3Hg(+) in toluene and back-extraction in 1% l-cysteine aqueous solution...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28499169/two-novel-dinuclear-ellipsoid-ni-ii-and-co-ii-complexes-bridged-by-4-5-bis-pyrazol-1-yl-phthalic-acid-synthesis-structural-characterization-and-biological-evaluation
#17
Enjun Gao, Zhenzhen Qi, Yun Qu, Yuqing Ding, Yang Zhan, Na Sun, Shaozhong Zhang, Xue Qiu, Mingchang Zhu
Two novel complexes, [Ni2(L)2(H2O)3]·4(H2O) (1) and [Co2(L)2(H2O)3]·5(H2O) (2) [H2L = 4,5-bis(pyrazol-1-yl) phthalic acid] were synthesized and characterized by spectroscopy (IR, (1)H NMR), and elemental analysis. The structures for the complexes were determined by X-ray crystallography providing the dinuclear ellipsoid Ni(II) and Co(II) complexes bridged by 4,5-bis(pyrazol-1-yl)phthalic acid ligands with same coordination modes. The interaction capacity of the complexes with FS-DNA (fish sperm DNA) has been investigated by UV and fluorescence spectroscopy...
May 4, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28496950/development-of-flow-cytometry-fluorescent-in-situ-hybridization-flow-fish-method-for-detection-of-pml-rara-chromosomal-translocation-in-acute-promyelocytic-leukemia-cell-line
#18
Fatemeh Zahedipour, Reza Ranjbaran, Abbas Behzad Behbahani, Khalil Tavakol Afshari, Mohammad Ali Okhovat, Gholamhossein Tamadon, Sedigheh Sharifzadeh
BACKGROUND: Acute Promyelocytic Leukemia (APL) is a subclass of acute myeloid leukemia. The chromosomal aberration in 95% of APL cases is t(15; 17) (q22; q21), which prevents cell differentiation. Characterization of the underlying molecular lesion is valuable in determining optimal treatment strategy. The goal of this study was to develop a new and powerful Flow- FISH technique to detect the long isoform (L) of PML-RARa fusion transcript in NB4 cell line. METHODS: To achieve the best condition for fixation, two different fixatives including 2% paraformaldehyde and 75% ethanol were used...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28494284/evaluating-the-impact-of-irrigation-on-surface-water-groundwater-interaction-and-stream-temperature-in-an-agricultural-watershed
#19
Hedeff I Essaid, Rodney R Caldwell
Changes in groundwater discharge to streams caused by irrigation practices can influence stream temperature. Observations along two currently flood-irrigated reaches in the 640-square-kilometer upper Smith River watershed, an important agricultural and recreational fishing area in west-central Montana, showed a downstream temperature decrease resulting from groundwater discharge to the stream. A watershed-scale coupled surface water and groundwater flow model was used to examine changes in streamflow, groundwater discharge to the stream and stream temperature resulting from irrigation practices...
May 7, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28489201/water-temperature-body-mass-and-fasting-heat-production-of-pacu-piaractus-mesopotamicus
#20
Fredy A A Aguilar, Thaline M P DA Cruz, Gerson B Mourão, José Eurico P Cyrino
Knowledge on fasting heat production (HEf) of fish is key to develop bioenergetics models thus improving feeding management of farmed species. The core of knowledge on HEf of farmed, neotropical fish is scarce. This study assessed the effect of body mass and water temperature on standard metabolism and fasting heat production of pacu, Piaractus mesopotamicus, an omnivore, Neotropical fresh water characin important for farming and fisheries industries all through South American continent. An automated, intermittent flow respirometry system was used to measure standard metabolic rate (SMR) of pacu (17 - 1,050 g) at five water temperatures: 19, 23, 26, 29 and 33 °C...
April 27, 2017: Anais da Academia Brasileira de Ciências
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