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https://www.readbyqxmd.com/read/28433716/development-of-a-monoclonal-antibody-against-the-cd3%C3%AE%C2%B5-of-olive-flounder-paralichthys-olivaceus-and-its-application-in-evaluating-immune-response-related-to-cd3%C3%AE%C2%B5
#1
Jae Wook Jung, Jung Seok Lee, Young Rim Kim, Se Pyeong Im, Si Won Kim, Jassy Mary S Lazarte, Jaesung Kim, Kim D Thompson, Jong Pyo Suh, Tae Sung Jung
The T cell receptor (TCR) is the binding site of antigen and is responsible for specifically activating the adaptive immune response. CD3, an essential component of the CD3-TCR complex, is known to be composed of γδ and ε chains in teleost. However, there are few monoclonal antibodies (mAb) available to identify these molecules on T cells, so we aimed to produce a mAb against CD3ε to improve our understanding of T cell immune response in olive flounder (Paralichthys olivaceus). CD3ε recombinant protein was expressed in yeast, the expression of which was confirmed by SDS-PAGE, MALDI-TOF/TOF MS and Western blot analysis...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28421268/using-a-population-model-to-inform-the-management-of-river-flows-and-invasive-carp-cyprinus-carpio
#2
John D Koehn, Charles R Todd, Brenton P Zampatti, Ivor G Stuart, Anthony Conallin, Leigh Thwaites, Qifeng Ye
Carp are a highly successful invasive fish species, now widespread, abundant and considered a pest in south-eastern Australia. To date, most management effort has been directed at reducing abundances of adult fish, with little consideration of population growth through reproduction. Environmental water allocations are now an important option for the rehabilitation of aquatic ecosystems, particularly in the Murray-Darling Basin. As carp respond to flows, there is concern that environmental watering may cause floodplain inundation and provide access to spawning habitats subsequently causing unwanted population increase...
April 18, 2017: Environmental Management
https://www.readbyqxmd.com/read/28414787/water-abstraction-affects-abundance-size-structure-and-growth-of-two-threatened-cyprinid-fishes
#3
Roberto Merciai, Carlota Molons-Sierra, Sergi Sabater, Emili García-Berthou
Hydrologic alteration is a major threat to freshwater biota, and particularly fish, in many river courses around the world. We analyzed and compared the effects of water abstraction on two threatened cyprinid fishes of contrasting ecology (the Mediterranean barbel Barbus meridionalis and the Catalan chub Squalius laietanus) in a Mediterranean stream. We compared abundance, size-structure, growth, and condition of both species across perennial and artificially intermittent reaches affected by water abstraction...
2017: PloS One
https://www.readbyqxmd.com/read/28414083/functional-characterization-of-neotropical-snakes-peripheral-blood-leukocytes-subsets-linking-flow-cytometry-cell-features-microscopy-images-and-serum-corticosterone-levels
#4
Marcelo Pires Nogueira de Carvalho, Nicolle Gilda Teixeira Queiroz-Hazarbassanov, Cristina de Oliveira Massoco, Sávio Stefanini Sant'Anna, Mariana Mathias Lourenço, Gabriel Levin, Mari Cleide Sogayar, Kathleen Fernandes Grego, José Luiz Catão-Dias
Reptiles are the unique ectothermic amniotes, providing the key link between ectothermic anamniotes fish and amphibians, and endothermic birds and mammals; becoming an important group to study with the aim of providing significant knowledge into the evolutionary history of vertebrate immunity. Classification systems for reptiles' leukocytes have been described by their appearance rather than function, being still inconsistent. With the advent of modern techniques and the establishment of analytical protocols for snakes' blood by flow cytometry, we bring a qualitative and quantitative assessment of innate activities presented by snakes' peripheral blood leukocytes, thereby linking flow cytometric features with fluorescent and light microscopy images...
April 13, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28412580/a-non-toxic-dose-of-cobalt-chloride-blocks-hair-cells-of-the-zebrafish-lateral-line
#5
William J Stewart, Jacob L Johansen, James C Liao
Experiments on the flow-sensitive lateral line system of fishes have provided important insights into the function and sensory transduction of vertebrate hair cells. A common experimental approach has been to pharmacologically block lateral line hair cells and measure how behavior changes. Cobalt chloride (CoCl2) blocks the lateral line by inhibiting calcium movement through the membrane channels of hair cells, but high concentrations can be toxic, making it unclear whether changes in behavior are due to a blocked lateral line or poor health...
April 12, 2017: Hearing Research
https://www.readbyqxmd.com/read/28399885/next-generation-sequencing-and-fish-studies-reveal-the-appearance-of-gene-mutations-and-chromosomal-abnormalities-in-hematopoietic-progenitors-in-chronic-lymphocytic-leukemia
#6
Miguel Quijada-Álamo, María Hernández-Sánchez, Cristina Robledo, Jesús-María Hernández-Sánchez, Rocío Benito, Adrián Montaño, Ana E Rodríguez-Vicente, Dalia Quwaider, Ana-África Martín, María García-Álvarez, María Jesús Vidal-Manceñido, Gonzalo Ferrer-Garrido, María-Pilar Delgado-Beltrán, Josefina Galende, Juan-Nicolás Rodríguez, Guillermo Martín-Núñez, José-María Alonso, Alfonso García de Coca, José A Queizán, Magdalena Sierra, Carlos Aguilar, Alexander Kohlmann, José-Ángel Hernández, Marcos González, Jesús-María Hernández-Rivas
BACKGROUND: Chronic lymphocytic leukemia (CLL) is a highly genetically heterogeneous disease. Although CLL has been traditionally considered as a mature B cell leukemia, few independent studies have shown that the genetic alterations may appear in CD34+ hematopoietic progenitors. However, the presence of both chromosomal aberrations and gene mutations in CD34+ cells from the same patients has not been explored. METHODS: Amplicon-based deep next-generation sequencing (NGS) studies were carried out in magnetically activated-cell-sorting separated CD19+ mature B lymphocytes and CD34+ hematopoietic progenitors (n = 56) to study the mutational status of TP53, NOTCH1, SF3B1, FBXW7, MYD88, and XPO1 genes...
April 11, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28396354/functional-diversity-of-the-lateral-line-system-among-populations-of-a-native-australian-freshwater-fish
#7
Lindsey Spiller, Pauline F Grierson, Peter M Davies, Jan Hemmi, Shaun P Collin, Jennifer L Kelley
Fishes use their mechanoreceptive lateral line system to sense nearby objects by detecting slight fluctuations in hydrodynamic motion within their immediate environment. Species of fishes from different habitats often display specialisations of the lateral line system, in particular the distribution and abundance of neuromasts, but the lateral line can also exhibit considerable diversity within a species. Here, we provide the first investigation of the lateral line system of the Australian western rainbowfish (Melanotaenia australis), a species that occupies a diversity of freshwater habitats across semi-arid northwest Australia...
April 10, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28394904/discordant-population-histories-of-host-and-its-parasite-a-role-for-ecological-permeability-of-extreme-environment
#8
Dagmar Jirsová, Jan Štefka, Miloslav Jirků
Biogeographical and ecological barriers strongly affect the course of micro-evolutionary processes in free living organisms. Here we assess the impact of a recently emerged barrier on populations of limnic fauna. Genetic diversity and population structure in a host-parasite system (Wenyonia virilis tapeworm, Synodontis schall catfish) are analyzed in the recently divided Turkana and Nile basins. The two basins, were repeatedly connected during the Holocene wet/dry climatic oscillations, following late Pleistocene dessication of the Turkana basin...
2017: PloS One
https://www.readbyqxmd.com/read/28386906/cd49d-associates-with-nodal-presentation-and-subsequent-development-of-lymphadenopathy-in-patients-with-chronic-lymphocytic-leukaemia
#9
Paolo Strati, Sameer A Parikh, Kari G Chaffee, Sara J Achenbach, Susan L Slager, Timothy G Call, Wei Ding, Diane F Jelinek, Curtis A Hanson, Neil E Kay, Tait D Shanafelt
CD49d is a surface integrin that is expressed on chronic lymphocytic leukaemia (CLL) cells, and strongly correlates with more aggressive disease. Given its association with cell-cell adhesion and leucocyte trafficking, we hypothesized that patients with high CD49d expression would experience a clinical course dominated by lymphadenopathy. CD49d expression was measured by flow cytometry and considered positive if expressed by ≥30% of CLL cells. The study included 797 newly diagnosed CLL/small lymphocytic leukaemia patients; 279 (35%) were CD49d positive...
April 7, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28386433/historical-gene-flow-constraints-in-a-northeastern-atlantic-fish-phylogeography-of-the-ballan-wrasse-labrus-bergylta-across-its-distribution-range
#10
Frederico Almada, Sara M Francisco, Cristina S Lima, Richard FitzGerald, Luca Mirimin, David Villegas-Ríos, Fran Saborido-Rey, Pedro Afonso, Telmo Morato, Sérgio Bexiga, Joana I Robalo
The distribution and demographic patterns of marine organisms in the north Atlantic were largely shaped by climatic changes during the Pleistocene, when recurrent glacial maxima forced them to move south or to survive in northern peri-glacial refugia. These patterns were also influenced by biological and ecological factors intrinsic to each species, namely their dispersion ability. The ballan wrasse (Labrus bergylta), the largest labrid fish along Europe's continental margins, is a target for fisheries and aquaculture industry...
February 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28382548/study-of-persistent-toxic-pollutants-in-a-river-basin-ecotoxicological-risk-assessment
#11
Evangelia Terzopoulou, Dimitra Voutsa
This study presents a complementary approach for the evaluation of water quality in a river basin by employing active and passive sampling. Persistent toxic pollutants representing three classes: organochlorinated pesticides (OCPs), polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs), were studied in grab water samples, in passive samplers/SemiPermeable Membrane Devices (SPMDs) and in fish tissues collected along the Strymonas River, northern Greece at three sampling campaigns during the year 2013...
April 5, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28376283/modeled-hydrologic-metrics-show-links-between-hydrology-and-the-functional-composition-of-stream-assemblages
#12
Christopher J Patrick, Lester L Yuan
Flow alteration is widespread in streams, but current understanding of the effects of differences in flow characteristics on stream biological communities is incomplete. We tested hypotheses about the effect of variation in hydrology on stream communities by using generalized additive models to relate watershed information to the values of different flow metrics at gauged sites. Flow models accounted for 54-80% of the spatial variation in flow metrics values among gauged sites. We then used these models to predict flow metrics in 842 ungauged stream sites within the mid-Atlantic, USA that were sampled for fish, macroinvertebrates, and environmental covariates...
April 4, 2017: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/28374227/predicting-the-influence-of-streamflow-on-migration-and-spawning-of-a-threatened-diadromous-fish-the-australian-grayling-prototroctes-maraena
#13
W M Koster, D A Crook, D R Dawson, S Gaskill, J R Morrongiello
The development of effective strategies to restore the biological functioning of aquatic ecosystems with altered flow regimes requires a detailed understanding of flow-ecology requirements, which is unfortunately lacking in many cases. By understanding the flow conditions required to initiate critical life history events such as migration and spawning, it is possible to mitigate the threats posed by regulated river flow by providing targeted environmental flow releases from impoundments. In this study, we examined the influence of hydrological variables (e...
April 3, 2017: Environmental Management
https://www.readbyqxmd.com/read/28367372/disturbed-flow-in-an-aquatic-environment-may-create-a-sensory-refuge-for-aggregated-prey
#14
Asa Johannesen, Alison M Dunn, Lesley J Morrell
Predators use olfactory cues moved within water and air to locate prey. Because prey aggregations may produce more cue and be easier to detect, predation could limit aggregation size. However, disturbance in the flow may diminish the reliability of odour as a prey cue, impeding predator foraging success and efficiency. We explore how different cue concentrations (as a proxy for prey group size) affect risk to prey by fish predators in disturbed (more turbulent or mixed) and non-disturbed (less mixed) flowing water...
2017: PeerJ
https://www.readbyqxmd.com/read/28363014/chromosomal-aberrations-in-large-japanese-field-mice-apodemus-speciosus-captured-near-fukushima-dai-ichi-nuclear-power-plant
#15
Taiki Kawagoshi, Naoko Shiomi, Hiroyuki Takahashi, Yoshito Watanabe, Shoichi Fuma, Kazutaka Doi, Isao Kawaguchi, Masanari Aoki, Masahide Kubota, Yoshiaki Furuhata, Yusaku Shigemura, Masahiko Mizoguchi, Fumio Yamada, Morihiko Tomozawa, Shinsuke H Sakamoto, Satoshi Yoshida, Yoshihisa Kubota
Since the Fukushima Dai-ichi Nuclear Power Plant accident, radiation effects on nonhuman biota in the contaminated areas have been a major concern. Here, we analyzed the frequencies of chromosomal aberrations (translocations and dicentrics) in the splenic lymphocytes of large Japanese field mice (Apodemus speciosus) inhabiting Fukushima Prefecture. A. speciosus chromosomes 1, 2, and 5 were flow-sorted in order to develop A. speciosus chromosome-specific painting probes, and FISH (fluorescence in situ hybridization) was performed using these painting probes to detect the translocations and dicentrics...
April 18, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28360007/biofilm-structures-eps-and-bacterial-communities-in-drinking-water-distribution-systems-are-conditioned-by-hydraulics-and-influence-discolouration
#16
K Fish, A M Osborn, J B Boxall
High-quality drinking water from treatment works is degraded during transport to customer taps through the Drinking Water Distribution System (DWDS). Interactions occurring at the pipe wall-water interface are central to this degradation and are often dominated by complex microbial biofilms that are not well understood. This study uses novel application of confocal microscopy techniques to quantify the composition of extracellular polymeric substances (EPS) and cells of DWDS biofilms together with concurrent evaluation of the bacterial community...
March 27, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28355166/synchronisation-through-learning-for-two-self-propelled-swimmers
#17
Guido Novati, Siddhartha Verma, Dmitry Alexeev, Diego Rossinelli, Wim M van Rees, Petros Koumoutsakos
The coordinated motion by multiple swimmers is a fundamental component in fish schooling. The flow field induced by the motion of each self-propelled swimmer implies non-linear hydrodynamic interactions among the members of a group. How do swimmers compensate for such hydrodynamic interactions in coordinated patterns? We provide an answer to this riddle though simulations of two, self-propelled, fish-like bodies that employ a learning algorithm to synchronise their swimming patterns. We distinguish between learned motion patterns and the commonly used a-priori specified movements, that are imposed on the swimmers without feedback from their hydrodynamic interactions...
March 29, 2017: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/28351541/nutrient-contributions-and-biogas-potential-of-co-digestion-of-feedstocks-and-dairy-manure
#18
Guiling Ma, J Shannon Neibergs, Joseph H Harrison, Elizabeth M Whitefield
This study focused on collection of data on nutrient flow and biogas yield at a commercial anaerobic digester managed with dairy manure from a 1000 cow dairy and co-digestion of additional feedstocks. Feedstocks included: blood, fish, paper pulp, out of date beverages and grease trap waste. Mass flow of inputs and outputs, nutrient concentration of inputs and outputs, and biogas yield were obtained. It was determined that manure was the primary source of nutrients to the anaerobic digester when co-digested with feedstocks...
March 25, 2017: Waste Management
https://www.readbyqxmd.com/read/28349255/alcohol-and-red-wine-consumption-but-not-fruit-vegetables-fish-or-dairy-products-are-associated-with-less-endothelial-dysfunction-and-less-low-grade-inflammation-the-hoorn-study
#19
B C T van Bussel, R M A Henry, C G Schalkwijk, J M Dekker, G Nijpels, E J M Feskens, C D A Stehouwer
PURPOSE: Endothelial dysfunction and low-grade inflammation are key phenomena in the pathobiology of cardiovascular disease (CVD). Their dietary modification might explain the observed reduction in CVD that has been associated with a healthy diet rich in fruit, vegetables and fish, low in dairy products and with moderate alcohol and red wine consumption. We investigated the associations between the above food groups and endothelial dysfunction and low-grade inflammation in a population-based cohort of Dutch elderly individuals...
March 27, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28346992/longitudinal-assessment-of-hydropeaking-impacts-on-various-scales-for-an-improved-process-understanding-and-the-design-of-mitigation-measures
#20
C Hauer, P Holzapfel, P Leitner, W Graf
Hydropeaking is one of the main pressures on the aquatic ecology in alpine rivers. Beside studies on abiotic process and biotic response on the local scale there is a lack in process understanding on the reach scale. Especially longitudinal changes of hydropeaking impacts based on retention processes have not been studied yet. Thus, based on unsteady one-dimensional and two-dimensional depth averaged modelling it was targeted to investigate possible changes in vertical ramping velocity for the discussion of possible mitigation measures at the local scale...
January 1, 2017: Science of the Total Environment
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