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Khamphone Yelithao, Utoomporn Surayot, Ju Hun Lee, SangGuan You
Water-soluble polysaccharides isolated from the rhizome of Polygonatum sibiricum and fractionated using ion-exchange chromatography were investigated to determine their structure and immunostimulating activity. Crude and fractions (F1 and F2) consisted of carbohydrates (85.1~88.3%) with proteins (4.51~11.9%) and uronic acid (1.79~7.47%), and included different levels of mannose (62.3~76.3%), glucose (15.2~20.3%), galactose (4.35~15.3%), and arabinose (4.00~7.65%). The crude contained two peaks with molecular weights (Mw) of 151×10(3) and 31...
September 2016: Preventive Nutrition and Food Science
Yang Si, Xueqin Wang, Chengcheng Yan, Liu Yang, Jianyong Yu, Bin Ding
Superelastic and pressure-sensitive carbonaceous nanofibrous aerogels with a honeycomb-like structure are fabricated through the combination of sustainable konjac glucomannan biomass and flexible SiO2 nanofibers. The aerogels can detect dynamic pressure with a wide pressure range and high sensitivity, which enables real pressure signals such as human blood pulses to be monitored in real time and in situ.
September 12, 2016: Advanced Materials
Yadu Nandan Dey, Garima Sharma, Manish M Wanjari, Dharmendra Kumar, Vinay Lomash, Ankush D Jadhav
CONTEXT: The tuber of Amorphophallus paeoniifolius (Dennst.) Nicolson (Araceae), commonly called Suran or Jimmikand, has high medicinal value and is used ethnomedicinally for the treatment of different gastrointestinal and inflammatory disorders. OBJECTIVE: The present study evaluated the effects of extracts of Amorphophallus paeoniifolius tubers on acetic acid-induced ulcerative colitis (UC) in rats. MATERIALS AND METHODS: Wistar rats were orally administered methanol extract (APME) or aqueous extract (APAE) (250 and 500 mg/kg) or standard drug, prednisolone (PRDS) (4 mg/kg) for 7 days...
September 7, 2016: Pharmaceutical Biology
Yuka Kojima, Anikó Várnai, Takuya Ishida, Naoki Sunagawa, Dejan M Petrovic, Kiyohiko Igarashi, Jody Jellison, Barry Goodell, Gry Alfredsen, Bjørge Westereng, Vincent G H Eijsink, Makoto Yoshida
: Fungi secrete a set of glycoside hydrolases and lytic polysaccharide monooxygenases (LPMOs) to degrade plant polysaccharides. Brown-rot fungi, such as Gloeophyllum trabeum, tend to have few LPMOs and information on these enzymes is scarce. The genome of G. trabeum encodes four AA9 LPMOs, whose coding sequences were amplified from cDNA. Due to alternative splicing, two variants of GtLPMO9A seem to be produced, a single domain variant, GtLPMO9A-1, and a longer variant, GtLPMO9A-2, which contains a C-terminal domain comprising approximately 55 residues without a predicted function...
September 2, 2016: Applied and Environmental Microbiology
Miao Yang, Jun Cai, Changgao Wang, Xin Du, Jianguo Lin
β-Mannanases are the second most important enzymes for the hydrolysis of hemicelluloses. An endo-β-mannanase from Enterobacter ludwigii MY271 was purified at 11.7 ± 0.2-fold to homogeneity with a final recovery of 15.2 ± 0.2 %. Using purified β-mannanase protein and SDS-PAGE, the molecular mass was found to be 43.16 kDa. The optimal pH and temperature of the enzyme was found to be 7.0 and 55 °C, respectively. The β-mannanase activity was stable over a broad pH range of pH 2.0-10.0. In addition, the purified enzyme was highly activated by several metal ions and chemical reagents, such as Mg(2+), L-cysteine, glutathione (GSH) and β-mercaptoethanol...
August 17, 2016: Bioprocess and Biosystems Engineering
Xuewen Ni, Fan Ke, Man Xiao, Kao Wu, Ying Kuang, Harold Corke, Fatang Jiang
Konjac glucomannan (KGM)-based aerogels were prepared using a combination of sol-gel and freeze-drying methods. Preparation conditions were chosen to control ice crystal growth and aerogel structure formation. The ice crystals formed during pre-freezing were observed by low temperature polarizing microscopy, and images of aerogel pores were obtained by scanning electron microscopy. The size of ice crystals were calculated and size distribution maps were drawn, and similarly for aerogel pores. Results showed that ice crystal growth and aerogel pore sizes may be controlled by varying pre-freezing temperatures, KGM concentration and glyceryl monostearate concentration...
November 2016: International Journal of Biological Macromolecules
Shuxin Ye, Weiping Jin, Qing Huang, Ying Hu, Yan Li, Bin Li
In this study, the preparation of magnetic Fe and Mn oxides (Mag-FMBO) loaded carbon aerogels (CA) based on konjac glucomannan (KGM) and their performance for dyes adsorption were investigated. The prepared magnetic carbon aerogels (Mag-CA) materials were characterized by various methods, including BET surface area analysis, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and vibrating sample magnetometer (VSM). What's more, the Mag-CA materials were used as adsorbents to remove dyes [anionic methyl orange (MO) and cationic methylene blue (MB)] from aqueous solutions...
November 2016: International Journal of Biological Macromolecules
Kais Fdhila, Najla Haddaji, Ibtissem Chakroun, Mohammed Ezz Eddine Macherki, Faten Nefzi, Amina Bakhrouf
We examine the effect of Glucomannan, extracted from Candida utilis yeast, on immune parameters and resistance to Vibrio splendidus of Crassostreagigas. Our results showed that Glucomannan was a successful anti-adhesive molecule; it exhibited a stronger inhibitory effect on adhesion of Vibrio splendidus in infected Crassostreagigas. Vibrio splendidus viable cells number declined after incubation with Glucomannan. Furthermore, the Glucomannan diet showed higher activity to trigger the immune response against bacteria...
September 2016: Fish & Shellfish Immunology
Yukako Okazaki, Hideyuki Chiji, Norihisa Kato
Lily bulb is traditionally consumed in East Asia and contains high amounts of glucomannan. This study investigated the effect of dietary lily bulb on dextran sulfate sodium (DSS)-induced colitis in rats fed a high-fat (HF) diet. Male Sprague-Dawley rats were fed a diet containing 30% beef tallow with or without 7% steamed lily bulb powder for 17 d. Experimental colitis was induced by replacing drinking water with DSS during the last 7 d. The disease activity index (DAI) was significantly lower in the lily bulb+DSS group than in the DSS group on day 17...
2016: Journal of Nutritional Science and Vitaminology
Mingrui Chen, Hongjie Wang, Qiuping Yan, Qiaoran Zheng, Min Yang, Zhenzhen Lv, Mei He, Limei Feng, Jiaqi Zhao, Tingting Tang, Yinglong Wu
In the present study, konjac glucomannan (KGM) was degraded by H2O2, and then used trisulfonated sodium amine and HCl, individually, to obtain two kinds of derivatives: oxidized konjac glucomannan sulfates (OKGMS) and acidolysis-oxidized konjac glucomannan (A-OKGM). The effects of two OKGM modified products on the immune parameters and expressions of toll-like receptor 22 (TLR22), myeloid differentiation factor 88 (MyD88) and interferon regulatory factors 7 (IRF7) genes in Schizothorax prenanti were determined...
September 2016: Fish & Shellfish Immunology
Xuewen Ni, Wenjie Chen, Man Xiao, Kao Wu, Ying Kuang, Harold Corke, Fatang Jiang
Konjac glucomannan (KGM)/ethyl cellulose (EC) could form a stable homogeneous emulsion with appropriate mixing formula. This study aimed to investigate the stable mechanism of KGM/EC emulsion at different mixing ratio through its rheological properties, and droplet size and morphology changes with up to 6days storage time. Though emulsions samples with high KGM content had larger droplet size and worse uniformity at the fresh stage, they appeared to be more stable and droplets growth rate were slow during storage...
July 5, 2016: International Journal of Biological Macromolecules
Lixia Wang, Yuanhong Zhuang, Jingliang Li, Jie Pang, Xiangyang Liu
Konjac glucomannan - tungsten (KGM-T) gels were successfully prepared under DC electric fields, in the presence of sodium tungstate. The textural properties and microstructure of the gels were investigated by Texture Analyzer, Rheometer and SEM. Based on the response surface methodology (RSM) results, the optimum conditions for KGM-T gel springiness is 0.32% sodium tungstate concentration, 0.54% KGM concentration, 24.66V voltage and 12.37min treatment time. Under these conditions, the maximum springiness value of KGM-T gel is 1...
December 1, 2016: Food Chemistry
Shuhong Dai, Harold Corke, Nagendra P Shah
Konjac glucomannan (KGM) has been reported to be beneficial to human health, as well as having potential functional properties as a fat replacer in dairy products. In this study, 0.5% KGM solution was added to prepare low-fat (LFKGM) and skimmed (SKKGM) yogurts, and their physicochemical properties were compared with those of full-fat yogurt control (FFC), low-fat yogurt control (LFC), and skimmed yogurt control (SKC). Properties and composition were determined and the microscopic structures of all yogurts were observed during storage at 4°C for 21d...
September 2016: Journal of Dairy Science
Mohammad Shah Hafez Kabir, Md Sofi Mahamoud, Nishan Chakrabarty, Shabbir Ahmad, Md Abdullah Al Masum, Md Akramul Hoque, Mohammed Munawar Hossain, Md Mominur Rahman, Mir Muhammad Nasir Uddin
BACKGROUND: This study aims to investigate whether tested organic extracts possess antithrombotic properties with minimal or no toxicity and to predict the activity of some of their isolated compounds. METHODS: An in vitro thrombolytic model was used to check the clot lysis effect of four Bangladeshi herbal extracts viz., roots of Curculigo recurvata W.T. Aiton (Satipata), leaf of Amorphophallus bulbifer Roxb. (Olkachu), leaf of Phyllanthus sikkimensis Muell. Arg...
July 2, 2016: Journal of Basic and Clinical Physiology and Pharmacology
Marta Busse-Wicher, An Li, Rodrigo L Silveira, Caroline S Pereira, Theodora Tryfona, Thiago C F Gomes, Munir S Skaf, Paul Dupree
The interaction between cellulose and xylan is important for the load-bearing secondary cell wall of flowering plants. Based on the precise, evenly spaced pattern of acetyl and glucuronosyl (MeGlcA) xylan substitutions in eudicots, we recently proposed that an unsubstituted face of xylan in a 2-fold helical screw can hydrogen bond to the hydrophilic surfaces of cellulose microfibrils. In gymnosperm cell walls, any role for xylan is unclear, and glucomannan is thought to be the important cellulose-binding polysaccharide...
August 2016: Plant Physiology
Wenjie Jian, Huayi Wu, Lanlan Wu, Yunhui Wu, Lina Jia, Jie Pang, Yuan-Ming Sun
Konjac glucomannan (KGM) is an important gelling agent in composite gels. This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP). In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics. Native KGM, 10kGy-KGM, and 20kGy-KGM exerted negative effect on gel strength or whiteness of TMP gels...
October 5, 2016: Carbohydrate Polymers
Congjiao Shi, Pei Zhu, Na Chen, Xiaozhou Ye, Yun Wang, Shaobo Xiao
Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery. In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers. By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV...
October 2016: International Journal of Biological Macromolecules
Charles G Winkworth-Smith, William MacNaughtan, Tim J Foster
The molten salt hydrate, lithium chloride (LiCl)/urea/water has previously been shown to swell cellulose, but there has so far been no work done to explore its effect on other polysaccharides. In this paper we have investigated the solvent effects of LiCl/urea/water on four natural polysaccharides. Fenugreek gum and xyloglucan, which are both highly branched, were found to increase in viscosity in LiCl/urea/water relative to water, possibly due to the breakage of all intra-molecular associations whereas the viscosity of konjac glucomannan which is predominantly unbranched did not change...
September 20, 2016: Carbohydrate Polymers
Nawapan Pongsapipatana, Piyanat Damrongteerapap, Sudathip Chantorn, Wilawan Sintuprapa, Suttipun Keawsompong, Sunee Nitisinprasert
Gene encoding for β-mannanase (E.C from Klebsiella oxytoca KUB-CW2-3 was cloned and expressed by an E. coli system resulting in 400 times higher mannanase activities than the wild type. A 3314bp DNA fragment obtained revealed an open reading frame of 1164bp, namely kman-2, which encoded for 387 amino acids with an estimated molecular weight of 43.2kDa. It belonged to the glycosyl hydrolase family 26 (GH26) exhibited low similarity of 50-71% to β-mannanase produced by other microbial sources. Interestingly, the enzyme had a broad range of substrate specificity of homopolymer of ivory nut mannan (6%), carboxymethyl cellulose (30...
July 2016: Enzyme and Microbial Technology
Jian-Xin Zhang, Ze-Tian Chen, Xiao-Lin Meng, Guang-Ya Mu, Wen-Bo Hu, Jie Zhao, Guo-Xing Nie
A endophyte with high yield of β-mannanase, designated as Paenibacillus sp. CH-3, was isolated from black locust (Robinia pseudoacacia) seeds. The β-mannanase gene (manB) has been cloned, expressed, purified and characterized. With an open reading frame of 984 bp, the manB encoding 327 amino acid residues, which belongs to GH family 5. The recombinant β-mannanase (ManB) has an apparent molecular weight of 50.4 kDa with an optimal activity level at pH 7.0 and 45°C, and is stable in the range from pH 4-9...
May 25, 2016: Biotechnology and Applied Biochemistry
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