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Glucomannan

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https://www.readbyqxmd.com/read/28193408/physicochemical-characterization-antioxidant-capacity-total-phenolic-and-proanthocyanidin-content-of-flours-prepared-from-pequi-caryocar-brasilense-camb-fruit-by-products
#1
Daniela P Leão, Adriana S Franca, Leandro S Oliveira, Rita Bastos, Manuel A Coimbra
The potential of pequi by-products as substrates for the production of flours rich in antioxidant dietary fibers was evaluated. Dietary fiber contents ranged from 39.8 to 43.3g/100g with pectic polysaccharide fraction constituted of rhamnogalacturonans and hemicellulose fraction consisted of arabinogalactans, xylans and glucomannans. Total polyphenols, non-extractable proanthocyanidins (NEPA) and carotenoid contents of the flours were determined (respectively, 15.5-17.4g GAE/100g, 215.54-346.84mg/100g and 2116...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28180916/fermentation-of-model-hemicelluloses-by-prevotella-strains-and-butyrivibrio-fibrisolvens-in-pure-culture-and-in-ruminal-enrichment-cultures
#2
Elianna L Emerson, Paul J Weimer
Hemicelluloses are major components of plant biomass, but their fermentation in the rumens of cattle and other ruminants is poorly understood. We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan). We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses...
February 8, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28169529/deacetylated-konjac-glucomannan-is-less-effective-in-reducing-dietary-induced-hyperlipidemia-and-hepatic-steatosis-in-c57bl-6-mice
#3
Meiying Li, Guanping Feng, Hong Wang, Ruili Yang, Zhenlin Xu, Yuanming Sun
Konjac gel foods that mainly consist of deacetylated konjac glucomannan (Da-KGM) are considered to have the same health benefits as native konjac glucomannan (KGM); however, no definitive data support this notion. The objective of this study was to compare the effects of Da-KGM and KGM on the hyperlipidemia and liver steatosis induced by high-fat diet feeding and to investigate the underlying molecular mechanisms. C57BL/6 mice were fed (1) normal chow diet; (2) high-fat diet; (3) HFD with KGM; (4) HFD with Da-KGM for 10 weeks...
February 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28132504/gelatin-based-nanocomplex-stabilized-pickering-emulsions-regulating-droplet-size-and-wettability-through-assembly-with-glucomannan
#4
Weiping Jin, Jieyu Zhu, Yike Jiang, Ping Shao, Bin Li, Qingrong Huang
Particle size and surface wettability play leading roles in the distribution of particles on the oil-water interface and the stability of emulsions. This work utilized nanocomplexes assembled from gelatin and tannic acid to stabilize Pickering emulsions. The sizes and surface wettability of particles were further regulated by using a polysaccharide. The sizes of nanocomplexes ranged from 205.8 to 422.2 nm and increased with the addition of polysaccharide. Their contact angles decreased from 84.1° to 59.3°, revealing their hydrophilic nature...
February 10, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28130129/heparin-mimetic-polyurethane-hydrogels-with-anticoagulant-tunable-mechanical-property-and-controllable-drug-releasing-behavior
#5
Yuan Chen, Rui Wang, Yonghui Wang, Weifeng Zhao, Shudong Sun, Changsheng Zhao
In the present study, novel heparin-mimetic polyurethane hydrogels were prepared by introducing chemical crosslinked sulfated konjac glucomannan (SKGM). Scanning electron microscopy (SEM) results indicated that the introduction of SKGM and the increase of the molecular weight of diol segments could enlarge the pore sizes of the hydrogels. The swelling behavior corresponded with the SEM results, and the hydrogels could absorb more water after the modification. The modification also led to an improvement in the mechanical property...
January 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28106808/anti-fatigue-effects-of-the-unique-polysaccharide-marker-of-dendrobium-officinale-on-balb-c-mice
#6
Wei Wei, Zhi-Peng Li, Tong Zhu, Hau-Yee Fung, Tin-Long Wong, Xin Wen, Dik-Lung Ma, Chung-Hang Leung, Quan-Bin Han
Dendrobium officinale extract shows potent anti-fatigue effects; however, the active substance responsible for these effects remains undetermined. A glucomannan with a huge molecular size of 730 kDa, called DOP, was identified as the unique authentication marker of this expensive herb. DOP exhibited immunomodulating effects on macrophages and lymphocytes in our previous study. Clinical reports also showed that people with fatigue syndrome have a disturbed immune system. Because DOP is the unique and dominant component of D...
January 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28105485/biochemical-characterization-of-thermostable-%C3%AE-1-4-mannanase-belonging-to-the-glycoside-hydrolase-family-134-from-aspergillus-oryzae
#7
Kiyota Sakai, Mai Mochizuki, Miyuki Yamada, Yuta Shinzawa, Miho Minezawa, Saran Kimoto, Shunsuke Murata, Yuhei Kaneko, Saaya Ishihara, Sadanari Jindou, Tetsuo Kobayashi, Masashi Kato, Motoyuki Shimizu
A β-1,4-mannanase, termed AoMan134A, that belongs to the GH 134 family was identified in the filamentous fungus Aspergillus oryzae. Recombinant AoMan134A was expressed in Pichia pastoris, and the purified enzyme produced mannobiose, mannotriose, mannotetraose, and mannopentaose from galactose-free β-mannan, with mannotriose being the predominant reaction product. The catalytic efficiency (k cat/K m ) of AoMan134A was 6.8-fold higher toward galactomannan from locust bean gum, than toward galactomannan from guar gum, but similar toward galactomannan from locust bean gum and glucomannan from konjac flour...
January 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28088615/cloning-heterologous-expression-and-biochemical-characterization-of-a-non-specific-endoglucanase-family-12-from-aspergillus-terreus-nih2624
#8
Fernando Segato, Bruno Dias, Gabriela L Berto, Dyoni M de Oliveira, Flávio H M De Souza, Ana Paula Citadini, Mario T Murakami, André R L Damásio, Fábio Márcio Squina, Igor Polikarpov
The cellulases from Glycoside Hydrolyses family 12 (GH12) play an important role in cellulose degradation and plant cell wall deconstruction being widely used in a number of bioindustrial processes. Aiming to contribute toward better comprehension of these class of the enzymes, here we describe a high-yield secretion of a endoglucanase GH12 from Aspegillus terreus (AtGH12), which was cloned and expressed in Aspergillus nidulans strain A773. The purified protein was used for complete biochemical and functional characterization...
January 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28038754/molecular-weight-distribution-and-fermentation-of-mechanically-pre-treated-konjac-enzymatic-hydrolysates
#9
Jun Yang, Natale Vittori, Weiwei Wang, Yong-Cheng Shi, Jennifer L Hoeflinger, Michael J Miller, Yang Pan
There is interest in novel fibers as potential prebiotics for new and reformulated food products. Two konjac glucomannan (KGM) hydrolysates were developed by enzymatic hydrolysis with (KGMH I) or without (KGMH II) mechanical shear pre-treatment. These were characterized and evaluated as fermentation substrates using five lactobacilli and three bifidobacteria. Enzymatic treatment of native KGM reduced the average molecular weights of supernatant and pellet by ∼3-fold. Additional mechanical shear pre-treatment further reduced supernatant and pellet molecular weights by 5% and 35%, respectively...
March 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27988000/co-loading-and-intestine-specific-delivery-of-multiple-antioxidants-in-ph-responsive-microspheres-based-on-tempo-oxidized-polysaccharides
#10
Mengxuan Shi, Jie Bai, Liyun Zhao, Xinrui Yu, Jingjing Liang, Ying Liu, Willem Nord, Yuan Li
In this study, pH-responsive microspheres loaded with multiple antioxidants were developed for intestine-specific delivery and exhibited synergistic activity. They consist of chitosan (CS)-coated microspheres made of TEMPO-oxidized Konjac glucomannan (OKGM) polymers, of which the carboxyl (COO(-)) groups are cross-linked via ferric ions (Fe(3+)), allowing the hydrophobic (β-carotene) and hydrophilic (anthocyanins) antioxidants to be simultaneously incorporated. CLSM images showed successful co-encapsulation of β-carotene and anthocyanins...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27872187/galactomannan-catabolism-conferred-by-a-polysaccharide-utilization-locus-of-bacteroides-ovatus-enzyme-synergy-and-crystal-structure-of-a-%C3%AE-mannanase
#11
Viktoria Bågenholm, Sumitha K Reddy, Hanene Bouraoui, Johan Morrill, Evelina Kulcinskaja, Constance M Bahr, Oskar Aurelius, Theresa Rogers, Yao Xiao, Derek T Logan, Eric C Martens, Nicole M Koropatkin, Henrik Stålbrand
A recently identified polysaccharide utilization locus (PUL) from Bacteroides ovatus ATCC 8483 is transcriptionally up-regulated during growth on galacto- and glucomannans. It encodes two glycoside hydrolase family 26 (GH26) β-mannanases, BoMan26A and BoMan26B, and a GH36 α-galactosidase, BoGal36A. The PUL also includes two glycan-binding proteins, confirmed by β-mannan affinity electrophoresis. When this PUL was deleted, B. ovatus was no longer able to grow on locust bean galactomannan. BoMan26A primarily formed mannobiose from mannan polysaccharides...
January 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27868067/purification-and-characterization-of-a-thermostable-%C3%AE-mannanase-from-bacillus-subtilis-be-91-potential-application-in-inflammatory-diseases
#12
Lifeng Cheng, Shengwen Duan, Xiangyuan Feng, Ke Zheng, Qi Yang, Zhengchu Liu
β-mannanase has shown compelling biological functions because of its regulatory roles in metabolism, inflammation, and oxidation. This study separated and purified the β-mannanase from Bacillus subtilis BE-91, which is a powerful hemicellulose-degrading bacterium using a "two-step" method comprising ultrafiltration and gel chromatography. The purified β-mannanase (about 28.2 kDa) showed high specific activity (79, 859.2 IU/mg). The optimum temperature and pH were 65°C and 6.0, respectively. Moreover, the enzyme was highly stable at temperatures up to 70°C and pH 4...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27842852/characteristics-of-glucomannan-isolated-from-fresh-tuber-of-porang-amorphophallus-muelleri-blume
#13
Anny Yanuriati, Djagal Wiseso Marseno, Rochmadi, Eni Harmayani
Porang is a potential source of glucomannan. This research objective was to find a direct glucomannan isolation method from fresh porang corm to produce high purity glucomannan. Two isolation methods were performed. In first method, sample was water dissolved using Al2(SO4)3 as flocculant for 15 (AA15) or 30 (AA30) minutes with purification. In second method, sample was repeatedly milled using ethanol as solvent and filtered for 5 (EtOH5) or 7 (EtOH7) times without purification. The characteristics of obtained glucomannan were compared to those of commercial porang flour (CPF) and purified konjac glucomannan (PKG)...
January 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27835835/effect-of-natural-antioxidants-in-spanish-salchich%C3%A3-n-elaborated-with-encapsulated-n-3-long-chain-fatty-acids-in-konjac-glucomannan-matrix
#14
P E S Munekata, R Domínguez, D Franco, R Bermúdez, M A Trindade, Jose M Lorenzo
The effect of natural antioxidants on physicochemical properties, lipid and protein oxidation, volatile compounds and free fatty acids (FFA) were determined in Spanish salchichón enriched with n-3 fatty acids encapsulated and stabilized in konjac matrix. Phenolic compounds of beer residue extract (BRE), chestnut leaves extract (CLE) and peanut skin extract (PSE) were also identified and quantified. Five batches of salchichón were prepared: control (CON, without antioxidants), butylated hydroxytoluene (BHT), BRE, CLE and PSE...
February 2017: Meat Science
https://www.readbyqxmd.com/read/27818862/kinetic-study-of-a-%C3%AE-mannanase-from-the-bacillus-licheniformis-hdym-04-and-its-decolorization-ability-of-twenty-two-structurally-different-dyes
#15
Jingping Ge, Renpeng Du, Dan Zhao, Gang Song, Man Jin, Wenxiang Ping
BACKGROUND: The microbial β-mannanases have been increasingly exploited for bioconversion of biomass materials and various potential industrial applications, such as bleaching of softwood pulps, scouring and desizing, food and feed additive, and oil and textile industries. In this paper, a β-mannanase was characterization from the bacteria, Bacillus licheniformis HDYM-04, which was a high β-mannanase-producing strain (576.16 ± 2.12 U/mL at 48 h during fermentation). METHODS: The michaelis constant (Km ) and maximum velocity (Vmax ) of β-mannanase were determined...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27771411/da-kgm-based-go-reinforced-fmbo-loaded-aerogels-for-efficient-arsenic-removal-in-aqueous-solution
#16
Shuxin Ye, Weiping Jin, Qing Huang, Ying Hu, Yan Li, Jing Li, Bin Li
Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water. Da-KGM, which was used as supporting composite matrix here, were firstly treated with GO and loaded FMBO. The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM)...
January 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27752501/raw264-7-cell-activating-glucomannans-extracted-from-rhizome-of-polygonatum-sibiricum
#17
Khamphone Yelithao, Utoomporn Surayot, Ju Hun Lee, SangGuan You
Water-soluble polysaccharides isolated from the rhizome of Polygonatum sibiricum and fractionated using ion-exchange chromatography were investigated to determine their structure and immunostimulating activity. Crude and fractions (F1 and F2) consisted of carbohydrates (85.1~88.3%) with proteins (4.51~11.9%) and uronic acid (1.79~7.47%), and included different levels of mannose (62.3~76.3%), glucose (15.2~20.3%), galactose (4.35~15.3%), and arabinose (4.00~7.65%). The crude contained two peaks with molecular weights (Mw) of 151×10(3) and 31...
September 2016: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/27615677/ultralight-biomass-derived-carbonaceous-nanofibrous-aerogels-with-superelasticity-and-high-pressure-sensitivity
#18
Yang Si, Xueqin Wang, Chengcheng Yan, Liu Yang, Jianyong Yu, Bin Ding
Superelastic and pressure-sensitive carbonaceous nanofibrous aerogels with a honeycomb-like structure are fabricated through the combination of sustainable konjac glucomannan biomass and flexible SiO2 nanofibers. The aerogels can detect dynamic pressure with a wide pressure range and high sensitivity, which enables real pressure signals such as human blood pulses to be monitored in real time and in situ.
September 12, 2016: Advanced Materials
https://www.readbyqxmd.com/read/27600166/beneficial-effect-of-amorphophallus-paeoniifolius-tuber-on-experimental-ulcerative-colitis-in-rats
#19
Yadu Nandan Dey, Garima Sharma, Manish M Wanjari, Dharmendra Kumar, Vinay Lomash, Ankush D Jadhav
CONTEXT: The tuber of Amorphophallus paeoniifolius (Dennst.) Nicolson (Araceae), commonly called Suran or Jimmikand, has high medicinal value and is used ethnomedicinally for the treatment of different gastrointestinal and inflammatory disorders. OBJECTIVE: The present study evaluated the effects of extracts of Amorphophallus paeoniifolius tubers on acetic acid-induced ulcerative colitis (UC) in rats. MATERIALS AND METHODS: Wistar rats were orally administered methanol extract (APME) or aqueous extract (APAE) (250 and 500 mg/kg) or standard drug, prednisolone (PRDS) (4 mg/kg) for 7 days...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27590806/characterization-of-an-lpmo-from-the-brown-rot-fungus-gloeophyllum-trabeum-with-broad-xyloglucan-specificity-and-its-action-on-cellulose-xyloglucan-complexes
#20
Yuka Kojima, Anikó Várnai, Takuya Ishida, Naoki Sunagawa, Dejan M Petrovic, Kiyohiko Igarashi, Jody Jellison, Barry Goodell, Gry Alfredsen, Bjørge Westereng, Vincent G H Eijsink, Makoto Yoshida
: Fungi secrete a set of glycoside hydrolases and lytic polysaccharide monooxygenases (LPMOs) to degrade plant polysaccharides. Brown-rot fungi, such as Gloeophyllum trabeum, tend to have few LPMOs and information on these enzymes is scarce. The genome of G. trabeum encodes four AA9 LPMOs, whose coding sequences were amplified from cDNA. Due to alternative splicing, two variants of GtLPMO9A seem to be produced, a single domain variant, GtLPMO9A-1, and a longer variant, GtLPMO9A-2, which contains a C-terminal domain comprising approximately 55 residues without a predicted function...
September 2, 2016: Applied and Environmental Microbiology
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