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Glucomannan

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https://www.readbyqxmd.com/read/28088615/cloning-heterologous-expression-and-biochemical-characterization-of-a-non-specific-endoglucanase-family-12-from-aspergillus-terreus-nih2624
#1
Fernando Segato, Bruno Dias, Gabriela L Berto, Dyoni M de Oliveira, Flávio H M De Souza, Matheus Augusto R N da Silva, Ana Paula Citadini, Mario T Murakami, André R L Damásio, Fábio Márcio Squina, Igor Polikarpov
The cellulases from Glycoside Hydrolyses family 12 (GH12) play an important role in cellulose degradation and plant cell wall deconstruction being widely used in a number of bioindustrial processes. Aiming to contribute toward better comprehension of these class of the enzymes, here we describe a high-yield secretion of a endoglucanase GH12 from Aspegillus terreus (AtGH12), which was cloned and expressed in Aspergillus nidulans strain A773. The purified protein was used for complete biochemical and functional characterization...
January 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28038754/molecular-weight-distribution-and-fermentation-of-mechanically-pre-treated-konjac-enzymatic-hydrolysates
#2
Jun Yang, Natale Vittori, Weiwei Wang, Yong-Cheng Shi, Jennifer L Hoeflinger, Michael J Miller, Yang Pan
There is interest in novel fibers as potential prebiotics for new and reformulated food products. Two konjac glucomannan (KGM) hydrolysates were developed by enzymatic hydrolysis with (KGMH I) or without (KGMH II) mechanical shear pre-treatment. These were characterized and evaluated as fermentation substrates using five lactobacilli and three bifidobacteria. Enzymatic treatment of native KGM reduced the average molecular weights of supernatant and pellet by ∼3-fold. Additional mechanical shear pre-treatment further reduced supernatant and pellet molecular weights by 5% and 35%, respectively...
March 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27988000/co-loading-and-intestine-specific-delivery-of-multiple-antioxidants-in-ph-responsive-microspheres-based-on-tempo-oxidized-polysaccharides
#3
Mengxuan Shi, Jie Bai, Liyun Zhao, Xinrui Yu, Jingjing Liang, Ying Liu, Willem Nord, Yuan Li
In this study, pH-responsive microspheres loaded with multiple antioxidants were developed for intestine-specific delivery and exhibited synergistic activity. They consist of chitosan (CS)-coated microspheres made of TEMPO-oxidized Konjac glucomannan (OKGM) polymers, of which the carboxyl (COO(-)) groups are cross-linked via ferric ions (Fe(3+)), allowing the hydrophobic (β-carotene) and hydrophilic (anthocyanins) antioxidants to be simultaneously incorporated. CLSM images showed successful co-encapsulation of β-carotene and anthocyanins...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27872187/galactomannan-catabolism-conferred-by-a-polysaccharide-utilization-locus-of-bacteroides-ovatus-enzyme-synergy-and-crystal-structure-of-a-%C3%AE-mannanase
#4
Viktoria Bågenholm, Sumitha K Reddy, Hanene Bouraoui, Johan Morrill, Evelina Kulcinskaja, Constance M Bahr, Oskar Aurelius, Theresa Rogers, Yao Xiao, Derek T Logan, Eric C Martens, Nicole M Koropatkin, Henrik Stålbrand
A recently identified polysaccharide utilization locus (PUL) from Bacteroides ovatus ATCC 8483 is transcriptionally up-regulated during growth on galacto- and glucomannans. It encodes two glycoside hydrolase family 26 (GH26) β-mannanases, BoMan26A and BoMan26B, and a GH36 α-galactosidase, BoGal36A. The PUL also includes two glycan-binding proteins, confirmed by β-mannan affinity electrophoresis. When this PUL was deleted, B. ovatus was no longer able to grow on locust bean galactomannan. BoMan26A primarily formed mannobiose from mannan polysaccharides...
January 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27868067/purification-and-characterization-of-a-thermostable-%C3%AE-mannanase-from-bacillus-subtilis-be-91-potential-application-in-inflammatory-diseases
#5
Lifeng Cheng, Shengwen Duan, Xiangyuan Feng, Ke Zheng, Qi Yang, Zhengchu Liu
β-mannanase has shown compelling biological functions because of its regulatory roles in metabolism, inflammation, and oxidation. This study separated and purified the β-mannanase from Bacillus subtilis BE-91, which is a powerful hemicellulose-degrading bacterium using a "two-step" method comprising ultrafiltration and gel chromatography. The purified β-mannanase (about 28.2 kDa) showed high specific activity (79, 859.2 IU/mg). The optimum temperature and pH were 65°C and 6.0, respectively. Moreover, the enzyme was highly stable at temperatures up to 70°C and pH 4...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27842852/characteristics-of-glucomannan-isolated-from-fresh-tuber-of-porang-amorphophallus-muelleri-blume
#6
Anny Yanuriati, Djagal Wiseso Marseno, Rochmadi, Eni Harmayani
Porang is a potential source of glucomannan. This research objective was to find a direct glucomannan isolation method from fresh porang corm to produce high purity glucomannan. Two isolation methods were performed. In first method, sample was water dissolved using Al2(SO4)3 as flocculant for 15 (AA15) or 30 (AA30) minutes with purification. In second method, sample was repeatedly milled using ethanol as solvent and filtered for 5 (EtOH5) or 7 (EtOH7) times without purification. The characteristics of obtained glucomannan were compared to those of commercial porang flour (CPF) and purified konjac glucomannan (PKG)...
January 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27835835/effect-of-natural-antioxidants-in-spanish-salchich%C3%A3-n-elaborated-with-encapsulated-n-3-long-chain-fatty-acids-in-konjac-glucomannan-matrix
#7
P E S Munekata, R Domínguez, D Franco, R Bermúdez, M A Trindade, Jose M Lorenzo
The effect of natural antioxidants on physicochemical properties, lipid and protein oxidation, volatile compounds and free fatty acids (FFA) were determined in Spanish salchichón enriched with n-3 fatty acids encapsulated and stabilized in konjac matrix. Phenolic compounds of beer residue extract (BRE), chestnut leaves extract (CLE) and peanut skin extract (PSE) were also identified and quantified. Five batches of salchichón were prepared: control (CON, without antioxidants), butylated hydroxytoluene (BHT), BRE, CLE and PSE...
February 2017: Meat Science
https://www.readbyqxmd.com/read/27818862/kinetic-study-of-a-%C3%AE-mannanase-from-the-bacillus-licheniformis-hdym-04-and-its-decolorization-ability-of-twenty-two-structurally-different-dyes
#8
Jingping Ge, Renpeng Du, Dan Zhao, Gang Song, Man Jin, Wenxiang Ping
BACKGROUND: The microbial β-mannanases have been increasingly exploited for bioconversion of biomass materials and various potential industrial applications, such as bleaching of softwood pulps, scouring and desizing, food and feed additive, and oil and textile industries. In this paper, a β-mannanase was characterization from the bacteria, Bacillus licheniformis HDYM-04, which was a high β-mannanase-producing strain (576.16 ± 2.12 U/mL at 48 h during fermentation). METHODS: The michaelis constant (Km ) and maximum velocity (Vmax ) of β-mannanase were determined...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27771411/da-kgm-based-go-reinforced-fmbo-loaded-aerogels-for-efficient-arsenic-removal-in-aqueous-solution
#9
Shuxin Ye, Weiping Jin, Qing Huang, Ying Hu, Yan Li, Jing Li, Bin Li
Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water. Da-KGM, which was used as supporting composite matrix here, were firstly treated with GO and loaded FMBO. The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM)...
January 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27752501/raw264-7-cell-activating-glucomannans-extracted-from-rhizome-of-polygonatum-sibiricum
#10
Khamphone Yelithao, Utoomporn Surayot, Ju Hun Lee, SangGuan You
Water-soluble polysaccharides isolated from the rhizome of Polygonatum sibiricum and fractionated using ion-exchange chromatography were investigated to determine their structure and immunostimulating activity. Crude and fractions (F1 and F2) consisted of carbohydrates (85.1~88.3%) with proteins (4.51~11.9%) and uronic acid (1.79~7.47%), and included different levels of mannose (62.3~76.3%), glucose (15.2~20.3%), galactose (4.35~15.3%), and arabinose (4.00~7.65%). The crude contained two peaks with molecular weights (Mw) of 151×10(3) and 31...
September 2016: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/27615677/ultralight-biomass-derived-carbonaceous-nanofibrous-aerogels-with-superelasticity-and-high-pressure-sensitivity
#11
Yang Si, Xueqin Wang, Chengcheng Yan, Liu Yang, Jianyong Yu, Bin Ding
Superelastic and pressure-sensitive carbonaceous nanofibrous aerogels with a honeycomb-like structure are fabricated through the combination of sustainable konjac glucomannan biomass and flexible SiO2 nanofibers. The aerogels can detect dynamic pressure with a wide pressure range and high sensitivity, which enables real pressure signals such as human blood pulses to be monitored in real time and in situ.
September 12, 2016: Advanced Materials
https://www.readbyqxmd.com/read/27600166/beneficial-effect-of-amorphophallus-paeoniifolius-tuber-on-experimental-ulcerative-colitis-in-rats
#12
Yadu Nandan Dey, Garima Sharma, Manish M Wanjari, Dharmendra Kumar, Vinay Lomash, Ankush D Jadhav
CONTEXT: The tuber of Amorphophallus paeoniifolius (Dennst.) Nicolson (Araceae), commonly called Suran or Jimmikand, has high medicinal value and is used ethnomedicinally for the treatment of different gastrointestinal and inflammatory disorders. OBJECTIVE: The present study evaluated the effects of extracts of Amorphophallus paeoniifolius tubers on acetic acid-induced ulcerative colitis (UC) in rats. MATERIALS AND METHODS: Wistar rats were orally administered methanol extract (APME) or aqueous extract (APAE) (250 and 500 mg/kg) or standard drug, prednisolone (PRDS) (4 mg/kg) for 7 days...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27590806/characterization-of-an-lpmo-from-the-brown-rot-fungus-gloeophyllum-trabeum-with-broad-xyloglucan-specificity-and-its-action-on-cellulose-xyloglucan-complexes
#13
Yuka Kojima, Anikó Várnai, Takuya Ishida, Naoki Sunagawa, Dejan M Petrovic, Kiyohiko Igarashi, Jody Jellison, Barry Goodell, Gry Alfredsen, Bjørge Westereng, Vincent G H Eijsink, Makoto Yoshida
: Fungi secrete a set of glycoside hydrolases and lytic polysaccharide monooxygenases (LPMOs) to degrade plant polysaccharides. Brown-rot fungi, such as Gloeophyllum trabeum, tend to have few LPMOs and information on these enzymes is scarce. The genome of G. trabeum encodes four AA9 LPMOs, whose coding sequences were amplified from cDNA. Due to alternative splicing, two variants of GtLPMO9A seem to be produced, a single domain variant, GtLPMO9A-1, and a longer variant, GtLPMO9A-2, which contains a C-terminal domain comprising approximately 55 residues without a predicted function...
September 2, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27534412/characterization-of-endo-%C3%AE-mannanase-from-enterobacter-ludwigii-my271-and-application-in-pulp-industry
#14
Miao Yang, Jun Cai, Changgao Wang, Xin Du, Jianguo Lin
β-Mannanases are the second most important enzymes for the hydrolysis of hemicelluloses. An endo-β-mannanase from Enterobacter ludwigii MY271 was purified at 11.7 ± 0.2-fold to homogeneity with a final recovery of 15.2 ± 0.2 %. Using purified β-mannanase protein and SDS-PAGE, the molecular mass was found to be 43.16 kDa. The optimal pH and temperature of the enzyme was found to be 7.0 and 55 °C, respectively. The β-mannanase activity was stable over a broad pH range of pH 2.0-10.0. In addition, the purified enzyme was highly activated by several metal ions and chemical reagents, such as Mg(2+), L-cysteine, glutathione (GSH) and β-mercaptoethanol...
January 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27521845/the-control-of-ice-crystal-growth-and-effect-on-porous-structure-of-konjac-glucomannan-based-aerogels
#15
Xuewen Ni, Fan Ke, Man Xiao, Kao Wu, Ying Kuang, Harold Corke, Fatang Jiang
Konjac glucomannan (KGM)-based aerogels were prepared using a combination of sol-gel and freeze-drying methods. Preparation conditions were chosen to control ice crystal growth and aerogel structure formation. The ice crystals formed during pre-freezing were observed by low temperature polarizing microscopy, and images of aerogel pores were obtained by scanning electron microscopy. The size of ice crystals were calculated and size distribution maps were drawn, and similarly for aerogel pores. Results showed that ice crystal growth and aerogel pore sizes may be controlled by varying pre-freezing temperatures, KGM concentration and glyceryl monostearate concentration...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27492560/kgm-based-magnetic-carbon-aerogels-matrix-for-the-uptake-of-methylene-blue-and-methyl-orange
#16
Shuxin Ye, Weiping Jin, Qing Huang, Ying Hu, Yan Li, Bin Li
In this study, the preparation of magnetic Fe and Mn oxides (Mag-FMBO) loaded carbon aerogels (CA) based on konjac glucomannan (KGM) and their performance for dyes adsorption were investigated. The prepared magnetic carbon aerogels (Mag-CA) materials were characterized by various methods, including BET surface area analysis, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and vibrating sample magnetometer (VSM). What's more, the Mag-CA materials were used as adsorbents to remove dyes [anionic methyl orange (MO) and cationic methylene blue (MB)] from aqueous solutions...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27492122/glucomannan-s-protective-effect-on-the-virulence-of-vibrio-splendidus-in-pacific-oyster
#17
Kais Fdhila, Najla Haddaji, Ibtissem Chakroun, Mohammed Ezz Eddine Macherki, Faten Nefzi, Amina Bakhrouf
We examine the effect of Glucomannan, extracted from Candida utilis yeast, on immune parameters and resistance to Vibrio splendidus of Crassostreagigas. Our results showed that Glucomannan was a successful anti-adhesive molecule; it exhibited a stronger inhibitory effect on adhesion of Vibrio splendidus in infected Crassostreagigas. Vibrio splendidus viable cells number declined after incubation with Glucomannan. Furthermore, the Glucomannan diet showed higher activity to trigger the immune response against bacteria...
September 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27465728/protective-effect-of-dietary-lily-bulb-on-dextran-sulfate-sodium-induced-colitis-in-rats-fed-a-high-fat-diet
#18
Yukako Okazaki, Hideyuki Chiji, Norihisa Kato
Lily bulb is traditionally consumed in East Asia and contains high amounts of glucomannan. This study investigated the effect of dietary lily bulb on dextran sulfate sodium (DSS)-induced colitis in rats fed a high-fat (HF) diet. Male Sprague-Dawley rats were fed a diet containing 30% beef tallow with or without 7% steamed lily bulb powder for 17 d. Experimental colitis was induced by replacing drinking water with DSS during the last 7 d. The disease activity index (DAI) was significantly lower in the lily bulb+DSS group than in the DSS group on day 17...
2016: Journal of Nutritional Science and Vitaminology
https://www.readbyqxmd.com/read/27394968/effects-of-dietary-oxidized-konjac-glucomannan-sulfates-okgms-and-acidolysis-oxidized-konjac-glucomannan-a-okgm-on-the-immunity-and-expression-of-immune-related-genes-of-schizothorax-prenanti
#19
Mingrui Chen, Hongjie Wang, Qiuping Yan, Qiaoran Zheng, Min Yang, Zhenzhen Lv, Mei He, Limei Feng, Jiaqi Zhao, Tingting Tang, Yinglong Wu
In the present study, konjac glucomannan (KGM) was degraded by H2O2, and then used trisulfonated sodium amine and HCl, individually, to obtain two kinds of derivatives: oxidized konjac glucomannan sulfates (OKGMS) and acidolysis-oxidized konjac glucomannan (A-OKGM). The effects of two OKGM modified products on the immune parameters and expressions of toll-like receptor 22 (TLR22), myeloid differentiation factor 88 (MyD88) and interferon regulatory factors 7 (IRF7) genes in Schizothorax prenanti were determined...
September 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27392771/physical-stability-and-rheological-properties-of-konjac-glucomannan-ethyl-cellulose-mixed-emulsions
#20
Xuewen Ni, Wenjie Chen, Man Xiao, Kao Wu, Ying Kuang, Harold Corke, Fatang Jiang
Konjac glucomannan (KGM)/ethyl cellulose (EC) could form a stable homogeneous emulsion with appropriate mixing formula. This study aimed to investigate the stable mechanism of KGM/EC emulsion at different mixing ratio through its rheological properties, and droplet size and morphology changes with up to 6days storage time. Though emulsions samples with high KGM content had larger droplet size and worse uniformity at the fresh stage, they appeared to be more stable and droplets growth rate were slow during storage...
November 2016: International Journal of Biological Macromolecules
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