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https://www.readbyqxmd.com/read/28213870/molecular-characterization-of-bovine-leukemia-virus-from-moldovan-dairy-cattle
#1
Aneta Pluta, Marzena Rola-Łuszczak, Piotr Kubiś, Svetlana Balov, Roman Moskalik, Bhudipa Choudhury, Jacek Kuźmak
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a disease that has worldwide distribution. Whilst it has been eradicated in most of Western Europe and Scandinavia, it remains a problem in other regions, particularly Eastern Europe and South America. For this study, in 2013, 24 cattle from three farms in three regions of Moldova were screened by ELISA and nested PCR. Of these cattle, 14 which were PCR positive, and these were molecularly characterized based on the nucleotide sequence of the env gene and the deduced amino acid sequence of the encoded gp51 protein...
February 17, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28207320/cardiac-findings-in-quarter-horses-with-heritable-equine-regional-dermal-asthenia
#2
Erin L Brinkman, Benjamin C Weed, Sourav S Patnaik, Bryn L Brazile, Ryan M Centini, Robert W Wills, Bari Olivier, Dodd G Sledge, Jim Cooley, Jun Liao, Ann M Rashmir-Raven
OBJECTIVE To compare biomechanical and histologic features of heart valves and echocardiographic findings between Quarter Horses with and without heritable equine regional dermal asthenia (HERDA). DESIGN Prospective case-control study. ANIMALS 41 Quarter Horses. PROCEDURES Ultimate tensile strength (UTS) of aortic and mitral valve leaflets was assessed by biomechanical testing in 5 horses with HERDA and 5 horses without HERDA (controls). Histologic evaluation of aortic and mitral valves was performed for 6 HERDA-affected and 3 control horses...
March 1, 2017: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/28206905/discovery-of-a-novel-alphavirus-related-to-eilat-virus
#3
Kyra Hermanns, Florian Zirkel, Anne Kopp, Marco Marklewitz, Innocent B Rwego, Alejandro Estrada, Thomas R Gillespie, Christian Drosten, Sandra Junglen
Most alphaviruses are transmitted by arthropods and infect vertebrate hosts. An exception is Eilat virus (EILV), the only described alphavirus with a host range restricted to insects. We established a new generic reverse transcription PCR assay for alphaviruses and tested 8860 tropical mosquitoes. We detected a novel alphavirus, tentatively named Taï Forest alphavirus (TALV), in Culex decens mosquitoes collected in Ivory Coast. The full genome was sequenced, and closest similarity was found to EILV. Pairwise amino acid identities to EILV ranged between 67 and 88 % for the corresponding proteins, suggesting that TALV defines a proposed new alphavirus species...
January 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28192048/a-serosurvey-of-diseases-of-free-ranging-gray-wolves-canis-lupus-in-minnesota
#4
Michelle Carstensen, John H Giudice, Erik C Hildebrand, J P Dubey, John Erb, Dan Stark, John Hart, Shannon Barber-Meyer, L David Mech, Steve K Windels, Andrew J Edwards
We tested serum samples from 387 free-ranging wolves ( Canis lupus ) from 2007 to 2013 for exposure to eight canid pathogens to establish baseline data on disease prevalence and spatial distribution in Minnesota's wolf population. We found high exposure to canine adenoviruses 1 and 2 (88% adults, 45% pups), canine parvovirus (82% adults, 24% pups), and Lyme disease (76% adults, 39% pups). Sixty-six percent of adults and 36% of pups exhibited exposure to the protozoan parasite Neospora caninum . Exposure to arboviruses was confirmed, including West Nile virus (37% adults, 18% pups) and eastern equine encephalitis (3% adults)...
February 13, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28190504/seroprevalence-and-risk-factors-for-infection-with-equine-coronavirus-in-healthy-horses-in-the-usa
#5
L J Kooijman, K James, S M Mapes, M J P Theelen, N Pusterla
Equine coronavirus (ECoV) is considered an enteric pathogen of foals and has only recently been associated with infections in adult horses. Seroprevalence data is needed to better understand the epidemiology of ECoV in adult horses, evaluate diagnostic modalities and develop preventive measures. The objective of this study was to investigate the seroprevalence and selective risk factors for ECoV in 5247 healthy adult horses in the USA, using a recently established and validated IgG enzyme-linked immunosorbent assay...
February 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28190496/evaluation-of-direct-etest-for-antimicrobial-susceptibility-testing-of-bacteria-isolated-from-synovial-fluid-of-horses-using-enrichment-bottles
#6
M Dumoulin, A Martens, A-M Van den Abeele, F Boyen, M Oosterlinck, H Wilderjans, F Gasthuys, F Haesebrouck, F Pille
This study evaluated the Etest for direct antimicrobial susceptibility testing (AST) of bacteria from equine synovial specimens, incubated in BACTEC enrichment bottles. Ninety-four culture-positive broths were inoculated onto agar to directly determine the minimum inhibitory concentrations (MICs) of 13 antimicrobials, using the Etest (direct Etest). Results were compared with those obtained with the agar dilution reference method, the standard Etest, and the disc diffusion method, after subculture and standardisation of the inoculum...
February 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28179180/developing-a-3-choice-serial-reaction-time-task-for-examining-neural-and-cognitive-function-in-an-equine-model
#7
Kirsty Roberts, Andrew J Hemmings, Sebastian D McBride, Matthew O Parker
BACKGROUND: Large animal models of human neurological disorders are advantageous compared to rodent models due to their neuroanatomical complexity, longevity and their ability to be maintained in naturalised environments. Some large animal models spontaneously develop behaviours that closely resemble the symptoms of neural and psychiatric disorders. The horse is an example of this; the domestic form of this species consistently develops spontaneous stereotypic behaviours akin to the compulsive and impulsive behaviours observed in human neurological disorders such as Tourette's syndrome...
February 5, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28178105/socket-preservation-using-a-biomimetic-nanostructured-matrix-and-atraumatic-surgical-extraction-technique
#8
Marco Mozzati, Giorgia Gallesio, Giorgio Staiti, Giovanna Iezzi, Adriano Piattelli, Carmen Mortellaro
OBJECTIVE: The aim of the present study was to evaluate the efficacy of biomimetic composite bone substitute composed of equine collagen I and Mg-hydroxyapatite in improving socket preservation after tooth extraction in humans. METHODS: Thirty-two patients were subjected to a single tooth extraction, performed without elevation of the full-thickness flap. In each patient, socket was grafted with the bone substitute and specimens were retrieved 2 months after surgery and processed for histological observations...
February 7, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28166929/kinetics-of-plasma-procalcitonin-soluble-cd14-ccl2-and-il-10-after-a-sublethal-infusion-of-lipopolysaccharide-in-horses
#9
Francesca Bonelli, Valentina Meucci, Thomas J Divers, Bettina Wagner, Luigi Intorre, Micaela Sgorbini
Endotoxemia represents a significant clinical and economic problem for the equine industry. This study assesses the kinetics of soluble CD14 (sCD14), chemokine (CC motif) ligand 2 (CCL2), interleukin 10 (IL-10) and plasma procalcitonin (PCT) in healthy horses after the intravenous infusion of lipopolysaccharide (LPS). The aim was to contribute to the basic understanding of the equine species-specific kinetics of these molecules in response to LPS exposure, which could support further findings in clinical studies and identify valuable inflammatory biomarkers for equine practice...
February 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28163276/update-of-inactivated-equine-influenza-vaccine-strain-in-japan
#10
Koichiro Gamoh, Shigeyuki Nakamura
Japan established a vaccine selection system, in which a committee evaluates veterinary influenza vaccines to determine if the vaccine should be updated. In 2013, it was concluded that the present equine influenza vaccine strains did not have to be updated, but clade 2 (Fc2) viruses of the Florida sublineage should be included. We collected three Fc2 viruses as candidates and conducted comparative tests. Results indicated that A/equine/Carlow/2011 (H3N8) is not suitable because of its unstable antigenic characteristics...
February 6, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28161600/equine-viral-arteritis-in-breeding-and-sport-horses-in-central-spain
#11
Fatima Cruz-Lopez, Richard Newton, Ana Sanchez-Rodriguez, Joanne Ireland, Lapo Mughini-Gras, Miguel A Moreno, Paloma Fores
Equine viral arteritis (EVA) may have a high economic impact on breeding stud farms due to the occurrence of EVA-associated abortion outbreaks and the ability of the virus to persist in carrier stallions. While the consequences of EVA in premises with sport horses are usually less severe, the first confirmed outbreak of EVA in Spain occurred in a riding club in Barcelona, but no data on the seroprevalence of EVA in sport horses have been reported in Spain. Given the importance of both Spanish Purebred (SP) breeding horses and sport horses for Spain's equine industry, the aim of this study was to determine and compare the seroprevalence of EVA in these two horse populations in central Spain...
January 27, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28161473/first-identification-and-phylogenetic-analysis-of-equine-hepacivirus-in-korea
#12
Ho-Seong Kim, Hyun-Woo Moon, Haan Woo Sung, Hyuk Moo Kwon
Non-primate hepacivirus (NPHV) corresponds a group of isolates recently characterized in horses and dogs that present similar genomic organization and are closely related to hepatitis C virus. Since canine hapacivirus, NPHV identified in dogs, was first discovered in dogs in the United States, equine hepacivirus (EqHV, NPHV identified in horses) has been identified in horses in several countries. However, no epidemiological studies have investigated EqHV in horses in Korea. In this study, a total of 74 (n=74) serum samples collected from horses in four regions of Korea were tested for EqHV RNA using nested RT-PCR...
February 1, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28160442/xenografting-of-isolated-equine-equus-caballus-testis-cells-results-in-de-novo-morphogenesis-of-seminiferous-tubules-but-not-spermatogenesis
#13
W Zeng, W Alpaugh, D Stefanovski, K Schlingmann, I Dobrinski, R M Turner
The study of spermatogenesis in the horse is challenging because of the absence of an in vitro system that is capable of reproducing efficient spermatogenesis and because of the difficulties and costs associated with performing well-controlled studies in vivo. In an attempt to develop novel methods for the study of equine spermatogenesis, we tested whether cells from enzymatically digested pre-pubertal equine testicular tissue were capable of de novo tissue formation and spermatogenesis following xenografting under the back skin of immunocompromised mice...
February 3, 2017: Andrology
https://www.readbyqxmd.com/read/28159238/in-vitro-effects-of-tamoxifen-on-equine-neutrophils
#14
C Borlone, N Morales, C Henriquez, H Folch, C Olave, J Sarmiento, B Uberti, G Moran
Neutrophils participate in innate immunity as the first line of host defense against microorganisms. However, exacerbated neutrophil activity can be harmful to surrounding tissues; this is important in a range of diseases, including allergic asthma and chronic obstructive pulmonary disease in humans, and equine asthma (also known as recurrent airway obstruction (RAO). Tamoxifen (TX) is a non-steroidal estrogen receptor modulator with effects on cell growth and survival. Previous preliminary studies showed that TX treatment in horses with induced acute pulmonary inflammation promoted early apoptosis of blood and BALF neutrophils, reduction of BALF neutrophils, and improvement in animals' clinical status...
February 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28158935/comparison-of-3-suture-closure-techniques-for-pelvic-flexure-enterotomy-in-equine-cadaveric-large-colon
#15
Ellison D Aldrich, Jennifer Earnest, Valerie J Moorman
OBJECTIVE: To compare a 2-layer closure with suture line reversal for a pelvic flexure enterotomy to 1-layer and traditional 2-layer hand sewn closures. STUDY DESIGN: Ex vivo, simple randomized study. SAMPLE POPULATION: Large colon segments from adult horses (n = 18). METHODS: Pelvic flexures were harvested from 18 horses and randomly assigned to 1 of 3 closure techniques (n = 6 per technique). A 10-cm enterotomy was made in each pelvic flexure and closed with the assigned technique...
February 3, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/28157811/one-stage-surgical-management-for-lumbar-brucella-spondylitis-by-posterior-debridement-autogenous-bone-graft-and-instrumentation-a-case-series-of-24-patients
#16
Yangbin Chen, Jun-Song Yang, Tao Li, Peng Liu, Tuan-Jiang Liu, Li-Min He, Li-Xiong Qian, Ding-Jun Hao
STUDY DESIGN: Clinical case series. OBJECTIVE: To explore the efficacy and safety of one-stage debridement, autogenous bone graft and instrumentation for lumbar brucella spondylitis (LBS) via a posterior approach. SUMMARY OF BACKGROUND DATA: Reports on LBS are sporadic, and the therapeutic effect and safety of surgical interventions have not been assessed in clinical studies. METHODS: Between January 2012 and January 2014, 24 consecutive patients with symptomatic LBS who underwent a one-stage operation that combined debridement, autogenous bone graft and instrumentation via a posterior approach were enrolled...
February 2, 2017: Spine
https://www.readbyqxmd.com/read/28146293/effect-of-fluid-media-on-the-mechanical-properties-of-continuous-pattern-ending-surgeon-s-square-and-aberdeen-knots-in-vitro
#17
Matthew Coleridge, Alexandra M Gillen, Ramsis Farag, R Reid Hanson, Amelia S Munsterman
OBJECTIVE: To investigate the knot holding capacity (KHC) of pattern-ending square, surgeon's and Aberdeen knots each tied in 4 throw combinations using large gauge suture after exposure to media commonly found in equine abdominal surgery. STUDY DESIGN: In vitro mechanical study. SAMPLE POPULATION: Knotted suture strands (n = 10/group). METHODS: Strands of 2 polydioxanone or 3 polyglactin 910 were exposed to 1 of 4 media for 15 minutes...
February 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/28132964/isolation-of-equine-herpesvirus-3-ehv-3-from-equine-coital-exanthema-of-two-stallions-and-sero-epidemiology-of-ehv-3-infection-in-japan
#18
Rikio Kirisawa, Yuko Toishi, Ai Akamatsu, Kosuke Soejima, Taisuke Miyashita, Nobuo Tsunoda
In the spring of 2015, two stallions reared in Farms A and B in Hokkaido in Japan showed symptoms of equine coital exanthema. Equine herpesvirus 3 (EHV-3) was isolated from penis swab samples of both stallions, and the isolates from each stallion in Farms A and B were designated as SS-1 and YS-1 strains, respectively. BamHI restriction profiles of SS-1 and Japanese reference strain Iwate-1 were indistinguishable, but the BamHI-A fragment of YS-1 was larger than those of SS-1 and Iwate-1 by 1.9 kbp because of the lack of two BamHI sites...
January 27, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28131384/seroprevalence-of-west-nile-virus-antibodies-in-equids-in-the-north-east-of-algeria-and-detection-of-virus-circulation-in-2014
#19
Ismail Lafri, Christine M Prat, Idir Bitam, Patrick Gravier, Mohamed Besbaci, Fayçal Zeroual, Meriem Hind Ben-Mahdi, Bernard Davoust, Isabelle Leparc-Goffart
West Nile fever (WNF) is a viral disease of wild birds transmitted by mosquitoes. Humans and equids can also be affected and suffer from meningoencephalitis. In Algeria, since the 1994 epidemic, no data on WNV circulation was available until 2012. In September 2012, a fatal human case of WNV neuro-invasive infection occurred in Jijel province. This study describes the first seroprevalence study of West Nile virus (WNV) antibodies conducted in the equine population in Algeria. During 2014, serum samples were collected from 293 equids (222 donkeys and 71 horses) asymptomatic and unvaccinated for WNV in three localities in Northeastern wetlands of Algeria...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28127617/an-anatomical-and-histological-study-of-the-equine-proximal-manica-flexoria
#20
Judith A Findley, Emanuele E Ricci, Ellen E Singer
OBJECTIVES: The main aim was to describe the gross and histological appearance of the equine manica flexoria and to identify any differences between the forelimbs and hindlimbs. An additional aim was to relate the findings to diagnostic and surgical anatomy of the manica flexoria. METHODS: Measurements of the manica flexoria were made on cadaveric limbs from horses free from pathology within the digital flexor tendon sheath. Histological sections, stained with haematoxylin and eosin and alcian- periodic acid schiff, were evaluated based on three micro-anatomical zones from dorsal to palmar or plantar...
January 27, 2017: Veterinary and Comparative Orthopaedics and Traumatology: V.C.O.T
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