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https://www.readbyqxmd.com/read/28191865/high-carbapenem-resistance-in-clinical-gram-negative-pathogens-isolated-in-egypt
#1
Hazim O Khalifa, Ahmed M Soliman, Ashraf M Ahmed, Toshi Shimamoto, Toshinori Hara, Mitsuyasu Ikeda, Yuta Kuroo, Shizuo Kayama, Motoyuki Sugai, Tadashi Shimamoto
The emergence and spread of carbapenem-resistant gram-negative bacteria poses a serious threat to human health worldwide. Currently, little is known about the molecular mechanisms underlying carbapenem resistance and their prevalence among gram-negative bacteria in Egypt. In this study, we analyzed carbapenemase production in gram-negative bacteria isolated from hospitalized patients in Egypt in 2014. All isolates were subjected to phenotypic and genotypic susceptibility testing for carbapenem resistance. Our results indicated a high level of carbapenem-resistant gram-negative bacteria in Egypt, with 50...
February 13, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28182124/extended-spectrum-beta-lactamase-and-carbapenemase-producing-enterobacteriaceae-among-ethiopian-children
#2
Melese Hailu Legese, Gebru Mulugeta Weldearegay, Daniel Asrat
BACKGROUND: Infections by extended-spectrum beta-lactamase- (ESBL) and carbapenem-resistant Enterobacteriaceae (CRE) are an emerging problem in children nowadays. Hence, the aim of this study was to determine the prevalence of ESBL- and carbapenemase-producing Enterobacteriaceae among children suspected of septicemia and urinary tract infections (UTIs). METHODS: A cross-sectional study was conducted from January to March 2014. A total of 322 study participants suspected of septicemia and UTIs were recruited...
2017: Infection and Drug Resistance
https://www.readbyqxmd.com/read/28167307/fosfomycin-in-vitro-efficacy-against-multidrug-resistant-isolates-beyond-urinary-isolates
#3
Tulin Demir, Tuncay Buyukguclu
OBJECTIVES: Fosfomycin (FOF) is a bactericidal antimicrobial agent active against a range of Gram-negative bacteria, including multidrug-resistant (MDR) and metallo-β-lactamase (MBL)-producing Enterobacteriaceae. However, data are scarce regarding use of the drug beyond urinary tract infections (UTIs). METHODS: In this study, susceptibility rates to FOF among 290 MDR Enterobacteriaceae isolates were analysed by gradient and disk diffusion tests and the results were compared with agar dilution according to the Clinical and Laboratory Standards Institute (CLSI)...
February 3, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28154285/fatal-rhabdomyolysis-caused-by-morganella-morganii-in-a-patient-with-multiple-myeloma
#4
Osamu Imataki, Makiko Uemura
A 64-year-old Japanese man with multiple myeloma was admitted to our institute due to fever and hypotension. He had received multiple courses of chemotherapy just before his febrile episode. Blood culturing detected Morganella morganii. At the time of the diagnosis, his laboratory findings revealed massive rhabdomyolysis with a significantly increased creatinine kinase level (CK; 3,582 U/L); 98.8% of which corresponded to the CK-MB isotype. We diagnosed the patient with sepsis caused by M. morganii, complicated with severe rhabdomyolysis...
2017: Internal Medicine
https://www.readbyqxmd.com/read/28112740/a-somatic-mutational-process-recurrently-duplicates-germline-susceptibility-loci-and-tissue-specific-super-enhancers-in-breast-cancers
#5
Dominik Glodzik, Sandro Morganella, Helen Davies, Peter T Simpson, Yilong Li, Xueqing Zou, Javier Diez-Perez, Johan Staaf, Ludmil B Alexandrov, Marcel Smid, Arie B Brinkman, Inga Hansine Rye, Hege Russnes, Keiran Raine, Colin A Purdie, Sunil R Lakhani, Alastair M Thompson, Ewan Birney, Hendrik G Stunnenberg, Marc J van de Vijver, John W M Martens, Anne-Lise Børresen-Dale, Andrea L Richardson, Gu Kong, Alain Viari, Douglas Easton, Gerard Evan, Peter J Campbell, Michael R Stratton, Serena Nik-Zainal
Somatic rearrangements contribute to the mutagenized landscape of cancer genomes. Here, we systematically interrogated rearrangements in 560 breast cancers by using a piecewise constant fitting approach. We identified 33 hotspots of large (>100 kb) tandem duplications, a mutational signature associated with homologous-recombination-repair deficiency. Notably, these tandem-duplication hotspots were enriched in breast cancer germline susceptibility loci (odds ratio (OR) = 4.28) and breast-specific 'super-enhancer' regulatory elements (OR = 3...
January 23, 2017: Nature Genetics
https://www.readbyqxmd.com/read/28110033/purification-characterization-and-functional-analysis-of-the-immune-molecule-lectin-from-the-haemolymph-of-blue-swimmer-crab-portunus-pelagicus-and-their-antibiofilm-properties
#6
Sangily Jayanthi, Ramachandran Ishwarya, Mahalingam Anjugam, Arokiadhas Iswarya, Sivashanmugam Karthikeyan, Baskaralingam Vaseeharan
The present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab Portunus pelagicus (Pp-Lec). The Pp-Lec was purified by affinity chromatography with mannose coupled sepharose CL-4B column and it exhibits single band with a molecular weight of 155 kDa in SDS-PAGE. The surface morphology of purified Pp-Lec displays the homogeneous nature of protein. A distinct peak with a retention time of 3.3 min was appeared in high performance liquid chromatography (HPLC) and X-ray diffraction (XRD) analysis expresses a single peak at 31...
January 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27984803/identification-of-new-bacteria-harboring-qnrs-and-aac-6-ib-cr-and-mutations-possibly-involved-in-fluoroquinolone-resistance-in-raw-sewage-and-activated-sludge-samples-from-a-full-scale-wwtp
#7
Magna C Paiva, Mariana P Reis, Patrícia S Costa, Marcela F Dias, Lucas Bleicher, Larissa L S Scholte, Regina M D Nardi, Andréa M A Nascimento
Wastewater treatment plants (WWTPs) harbor bacteria and antimicrobial resistance genes, favoring gene exchange events and resistance dissemination. Here, a culture-based and metagenomic survey of qnrA, qnrB, qnrS, and aac(6')-Ib genes from raw sewage (RS) and activated sludge (AS) of a full-scale municipal WWTP was performed. A total of 96 bacterial isolates were recovered from nalidixic acid-enrichment cultures. Bacteria harboring the aac(6')-Ib gene predominated in RS, whereas qnrS-positive isolates were specific to AS...
November 27, 2016: Water Research
https://www.readbyqxmd.com/read/27927090/antioxidant-anticholinesterase-and-antibacterial-activities-of-stachys-guyoniana-and-mentha-aquatica
#8
Maria Ferhat, Ebru Erol, Khadidja Aya Beladjila, Yunus Çetintaş, Mehmet Emin Duru, Mehmet Öztürk, Ahmed Kabouche, Zahia Kabouche
CONTEXT: Stachys guyoniana Noë ex. Batt. and Mentha aquatica L. are two Algerian Lamiaceae used in folk medicine. OBJECTIVE: To investigate their antioxidant, anticholinesterase and antibacterial activities. MATERIAL AND METHODS: n-Butanol (BESG), ethyl acetate (EESG) and chloroform (CESG) extracts of S. guyoniana and methanol (MEMA) and chloroform (CEMA) aerial part extracts of M. aquatica and methanol (MERMA) and acetone (AERMA) roots extracts of M...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27826290/draft-genomic-analysis-of-an-avian-multidrug-resistant-morganella-morganii-isolate-carrying-qnrd1
#9
Daniela Jones-Dias, Lurdes Clemente, Inês B Moura, Daniel A Sampaio, Teresa Albuquerque, Luís Vieira, Vera Manageiro, Manuela Caniça
Morganella morganii is a commensal bacterium and opportunistic pathogen often present in the gut of humans and animals. We report the 4.3 Mbp draft genome sequence of a M. morganii isolated in association with an Escherichia coli from broilers in Portugal that showed macroscopic lesions consistent with colisepticemia. The analysis of the genome matched the multidrug resistance phenotype and enabled the identification of several clinically important and potentially mobile acquired antibiotic resistance genes, including the plasmid-mediated quinolone resistance determinant qnrD1...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27818649/in-vivo-acquisition-of-carbapenemase-gene-blakpc-2-in-multiple-species-of-enterobacteriaceae-through-horizontal-transfer-of-insertion-sequence-or-plasmid
#10
Baixing Ding, Zhen Shen, Fupin Hu, Meiping Ye, Xiaogang Xu, Qinglan Guo, Minggui Wang
Objectives: Current worldwide spread of carbapenem resistance in Enterobacteriaceae constitutes a critical public health threat. This study aims to investigate how carbapenem resistance is acquired in Enterobacteriaceae in patients during antimicrobial therapy. Methods: Clinical strains from the same anatomical site of the same patients that converted from carbapenem-susceptible to resistant during antimicrobial therapy and showed identical or similar PFGE patterns were identified. The similarly sized plasmids carried by the susceptible and resistant strains, the latter containing the carbapenemase genes, were sequenced and analyzed...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27734638/pattern-of-urinary-antibiograms-in-a-tertiary-care-hospital-of-eastern-india
#11
Nandini Chatterjee, Chandan Chatterjee, Sinjan Ghosh, Mainak Mukhopadhyay, Ramkrishna Brahmachari, Kartik Patar
BACKGROUND: Indiscriminate use of antibiotics in urinary tract infections have led to emergence of 'superbugs' worldwide. Periodical review of antibiograms is of utmost importance for optimum patient benefit. OBJECTIVE: To identify the spectrum of organisms responsible for urinary tract infection and evaluate the pattern of antibiotic sensitivity of the organisms. METHODS: Urine samples were collected from all consecutive patients getting admitted in the medicine indoor irrespective of symptomatology...
April 2016: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/27720545/infectious-bacterial-pathogens-parasites-and-pathological-correlations-of-sewage-pollution-as-an-important-threat-to-farmed-fishes-in-egypt
#12
Mahmoud A Mahmoud, Mohamed Abdelsalam, Olfat A Mahdy, Hala M F El Miniawy, Zakia A M Ahmed, Ahmed H Osman, Hussein M H Mohamed, A M Khattab, M A Zaki Ewiss
This paper is a part of a multi-disciplinary research "Application of Decentralized On-Site Water Treatment System in Egypt for Use in Agriculture and Producing Safe Fish and Animal Proteins". The project aimed to investigate the environmental impact of implementing sewage water before and after treatment using the effluent of the on-site decentralized Japanese' Johkasou system, in agriculture and producing fish protein. The aim is to establish such system in Egypt to strengthen the sanitary conditions of water resources...
December 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27679611/architecture-of-class-1-2-and-3-integrons-from-gram-negative-bacteria-recovered-among-fruits-and-vegetables
#13
Daniela Jones-Dias, Vera Manageiro, Eugénia Ferreira, Paula Barreiro, Luís Vieira, Inês B Moura, Manuela Caniça
The spread of antibiotic resistant bacteria throughout the food chain constitutes a public health concern. To understand the contribution of fresh produce in shaping antibiotic resistance bacteria and integron prevalence in the food chain, 333 antibiotic resistance Gram negative isolates were collected from organic and conventionally produced fruits (pears, apples, and strawberries) and vegetables (lettuces, tomatoes, and carrots). Although low levels of resistance have been detected, the bacterial genera identified in the assessed fresh produce are often described not only as environmental, but mostly as commensals and opportunistic pathogens...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27635011/draft-genome-sequences-of-histamine-producing-morganella-psychrotolerans-strains
#14
Kristin Bjornsdottir-Butler, Maria Sanchez Leon, Ronald A Benner
Histamine-producing bacteria are responsible for scombrotoxin (histamine) fish poisoning, a leading cause of fish poisoning in the United States. We report here the first draft genomes of three histamine-producing Morganella psychrotolerans strains, isolated from tuna and mahi-mahi.
2016: Genome Announcements
https://www.readbyqxmd.com/read/27516567/can-surgical-need-in-patients-with-naja-atra-taiwan-or-chinese-cobra-envenomation-be-predicted-in-the-emergency-department
#15
H Y Su, M J Wang, Y H Li, C N Tang, M J Tsai
OBJECTIVES: To investigate the clinical predictors and the aetiologies for surgery in patients with Naja atra (Taiwan or Chinese cobra) envenomation. METHODS: This case series was conducted in the only tertiary care centre in eastern Taiwan. Patients who presented to the emergency department with Naja atra bite between January 2008 and September 2014 were included. Clinical information was collected and compared between surgical and non-surgical patients. RESULTS: A total of 28 patients with Naja atra envenomation presented to the emergency department during the study period...
October 2016: Hong Kong Medical Journal, Xianggang Yi Xue za Zhi
https://www.readbyqxmd.com/read/27504288/biofilm-in-osteomyelitis-caused-by-a-rare-pathogen-morganella-morganii-a-case-report
#16
Asmita De, Hirak Jyoti Raj, Prasanta Kumar Maiti
Morganella morganii is a member of Enterobacteriaceae family, whose natural habitat is the human gastrointestinal tract. It rarely causes infection alone and is generally encountered in immunosuppressed patients. Osteoarticular pathologies are not commonly observed with Morganella morganii and infections by it have high mortality rate. Biofilm colonization is a causative factor behind the chronicity and/or refractoriness of certain infections. Biofilms colonize on inert medical devices, prosthesis, fibrosed tissues, sinus tracts as well as dead bones as in case of chronic osteomyelitis...
June 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27496460/influence-of-double-j-catheters-on-urinary-infections-after-kidney-transplantation
#17
M Jonas, A Jóźwik, D Kawecki, M Durlik, L Pączek, G Młynarczyk, A Chmura
BACKGROUND: One of the most important problem in kidney transplantation is risk of the urinary anastomosis stricture. In uncertain cases the use of a double-J (or JJ) catheter is a standard solution. In case of urinary leak or fistula after the reanastomosis operation, transuretheral JJ implantation is used. A number of patients after JJ use present urinary infection. METHODS: Between 2012 and 2014, 283 kidney transplantations were performed in our center. In 77 cases (27...
June 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27495005/severe-chronic-osteomyelitis-caused-by-morganella-morganii-with-high-population-diversity
#18
Jialiang Zhu, Haifeng Li, Li Feng, Min Yang, Ronggong Yang, Lin Yang, Li Li, Ruoyan Li, Minshan Liu, Shuxun Hou, Yuehua Ke, Wenfeng Li, Fan Bai
A case of chronic osteomyelitis probably caused by Morganella morganii, occurring over a period of 30 years, is reported. The organism was identified through a combination of sample culture, direct sequencing, and 16S RNA gene amplicon sequencing. Further whole-genome sequencing and population structure analysis of the isolates from the patient showed the bacterial population to be highly diverse. This case provides a valuable example of a long-term infection caused by an opportunistic pathogen, M. morganii, with high diversity, which might evolve during replication within the host...
September 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27459019/clonal-transmission-of-gram-negative-bacteria-with-carbapenemases-ndm-1-vim-1-and-oxa-23-72-in-a-bulgarian-hospital
#19
Yvonne Pfeifer, Angelina Trifonova, Michael Pietsch, Magdalena Brunner, Iva Todorova, Ivanka Gergova, Gottfried Wilharm, Guido Werner, Encho Savov
We characterized 72 isolates with reduced susceptibility to carbapenems (50 Acinetobacter spp., 13 Proteus mirabilis, five Escherichia coli, one Morganella morganii, one Enterobacter cloacae, one Providencia rettgeri, and one Pseudomonas aeruginosa) from a hospital in Sofia, Bulgaria. Different β-lactamase genes were identified by polymerase chain reaction and sequencing. Bacterial strain typing was performed by enzymatic macrorestriction and pulsed-field gel electrophoresis (PFGE) typing as well as multilocus sequence typing for selected isolates...
July 26, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27433071/persistent-infection-with-metallo-beta-lactamase-and-extended-spectrum-%C3%AE-lactamase-producer-morganella-morganii-in-a-patient-with-urinary-tract-infection-after-kidney-transplantation
#20
Hamed Ebrahimzadeh Leylabadlo, Hossein Samadi Kafil, Mehdi Yousefi, Mohammad Aghazadeh, Mohammad Asgharzadeh
Organ transplant recipients under immunosuppressive therapy have a highly increased risk of acquiring unusual opportunistic infections. Diagnosis of the etiology of infection may be difficult in clinical manifestations, which need further histological and biological investigations. Here in we report, for the 1(st) time in the Iran, a Morganella morganii isolate harboring blaVIM, blaCTX-M, and blaSHV genes after kidney transplantation with persistent urinary infections.
July 2016: Journal of Natural Science, Biology, and Medicine
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