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https://www.readbyqxmd.com/read/28733908/assessment-of-cultivable-oral-bacterial-flora-from-important-venomous-snakes-of-india-and-their-antibiotic-susceptibilities
#1
Innus K Shaikh, Prashant P Dixit, Balasaheb S Pawade, Mugdha Potnis-Lele, Babasaheb P Kurhe
Snakebite is a common, frequently devastating, occupational, socio-economic hazard, and it has a great impact on the rural population of India. Snakebite is a major cause of the human morbidity and mortality since ancient times, as it not only affects the victim by systemic envenomation but also by wound infections originating from deadly pathogenic microorganisms from the oral cavity of the offending snake. The pathogens from the oral cavity of the snake tend to initiate an infection, resulting in gas gangrene, soft tissue necrosis, and permanent physical disabilities...
July 21, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28705499/development-of-silver-nanoparticles-loaded-chitosan-alginate-constructs-with-biomedical-potentialities
#2
Muhammad Bilal, Tahir Rasheed, Hafiz M N Iqbal, Chuanlong Li, Hongbo Hu, Xuehong Zhang
Herein, a facile biosynthesis of silver nanoparticles (AgNPs) and AgNPs-loaded chitosan-alginate constructs with biomedical potentialities is reported. The UV-vis spectroscopic profile confirmed the synthesis of AgNPs using methanolic leaves extract of Euphorbia helioscopia. The newly developed AgNPs were characterized using various analytical and imaging techniques including UV-vis and FT-IR spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), atomic force microscopy (AFM), and transmission electron microscopy (TEM)...
July 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28698604/honeybee-apis-mellifera-associated-bacterial-community-affected-by-american-foulbrood-detection-of-paenibacillus-larvae-via-microbiome-analysis
#3
Tomas Erban, Ondrej Ledvinka, Martin Kamler, Marta Nesvorna, Bronislava Hortova, Jan Tyl, Dalibor Titera, Martin Markovic, Jan Hubert
Honeybee (Apis mellifera L.) workers act as passive vectors of Paenibacillus larvae spores, which cause the quarantine disease American foulbrood (AFB). We assessed the relative proportions of P. larvae within the honeybee microbiome using metabarcoding analysis of the 16 S rRNA gene. The microbiome was analyzed in workers outside of the AFB zone (control - AFB0), in workers from asymptomatic colonies in an AFB apiary (AFB1), and in workers from colonies exhibiting clinical AFB symptoms (AFB2). The microbiome was processed for the entire community and for a cut-off microbiome comprising pathogenic/environmental bacteria following the removal of core bacterial sequences; varroosis levels were considered in the statistical analysis...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28696146/heat-resistance-of-histidine-decarboxylase-from-gram-negative-histamine-producing-bacteria-in-seafood
#4
K Bjornsdottir-Butler, F A Bencsath, S McCarthy, R A Benner
Precooking of tuna is a potential critical control point (CCP) in the commercial manufacturing of canned tuna. To assess the efficacy of precooking as a CCP, an understanding of the thermal properties of histamine-producing bacteria (HPB) and their histidine decarboxylase (HDC) enzymes is required. The thermal properties of many HPB have been determined, but the thermal resistances of the HDC enzymes are unknown. The purpose of this study was to determine the D- and z-values of selected HDC enzymes to evaluate the CCP of precooking during the canning process and provide scientific data to support U...
July 11, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28665245/prophylactic-use-of-intraoperative-vancomycin-powder-and-postoperative-infection-an-analysis-of-microbiological-patterns-in-1200-consecutive-surgical-cases
#5
Owoicho Adogwa, Aladine A Elsamadicy, Amanda Sergesketter, Victoria D Vuong, Ankit I Mehta, Raul A Vasquez, Joseph Cheng, Carlos A Bagley, Isaac O Karikari
OBJECTIVE Wound infections following spinal surgery for deformity place a high toll on patients, providers, and the health care system. The prophylactic application of intraoperative vancomycin powder has been shown to lower the infection risk after thoracolumbar decompression and fusion for deformity correction. The purpose of this study was to assess the microbiological patterns of postoperative surgical site infections (SSIs) after prophylactic use of vancomycin powder in adult patients undergoing spinal deformity surgery...
June 30, 2017: Journal of Neurosurgery. Spine
https://www.readbyqxmd.com/read/28663705/investigation-of-gut-microbial-communities-associated-with-indigenous-honey-bee-apis-mellifera-jemenitica-from-two-different-eco-regions-of-saudi-arabia
#6
Khalid Ali Khan, Mohammad Javed Ansari, Ahmad Al-Ghamdi, Adgaba Nuru, Steve Harakeh, Javaid Iqbal
The microbial communities associated with the alimentary tract of honey bees are very important as they help with food digestion, provide essential nutrients, protect the host from pathogens, detoxify harmful molecules, and increase host immunity. In this study, the structural diversity of the gut microbial communities of native honey bees, Apis mellifera jemenitica from two different geographical regions (Riyadh and Al-Baha) of Saudi Arabia was analyzed by culture-dependent methods and 16S ribosomal RNA (rRNA) gene sequencing...
July 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28659135/bilateral-morganella-morganii-keratitis-in-a-patient-with-facial-topical-corticosteroid-induced-rosacea-like-dermatitis-a-case-report
#7
Bei Zhang, Fei Pan, Kejian Zhu
BACKGROUND: Bilateral keratitis rarely occurs in individuals without predisposing factors. Here we describe the clinical course of a patient who developed a bilateral keratitis caused by Morganella. morganii which might be associated with long term using of topical corticosteroids-containing preparations on the face. CASE PRESENTATION: A 52-year-old female patient presented with marked bilateral corneal infiltration and hypopyon without any usual predisposing factors for bilateral infectious keratitis...
June 28, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28658333/bacterial-genus-is-a-risk-factor-for-major-amputation-in-patients-with-diabetic-foot
#8
Natália Anício Cardoso, Lígia DE Loiola Cisneiros, Carla Jorge Machado, Juliana Merlin Cenedezi, Ricardo Jayme Procópio, Túlio Pinho Navarro
Objective: to evaluate whether bacterial genus is a risk factor for major amputation in patients with diabetic foot and infected ulcer. Methods: we conducted a case-control, observational study of 189 patients with infected ulcers in diabetic feet admitted to the Vascular Surgery Service of the Risoleta Tolentino Neves Hospital, from January 2007 to December 2012. The bacteriological evaluation was performed in deep tissue cultures from the lesions and amputation was considered major when performed above the foot'smiddle tarsus...
March 2017: Revista do Colégio Brasileiro de Cirurgiões
https://www.readbyqxmd.com/read/28619802/draft-genome-sequences-of-extended-spectrum-beta-lactamase-producing-morganella-morganii-strains-aa1-and-av1-isolated-from-a-freshwater-lake-and-eicchorniacrassipes-roots
#9
Pushpa Lata, Subramaniam S Govindarajan, Feng Qi, Jian-Liang Li, Santosh K Maurya, Malaya K Sahoo
Two strains of Morganella morganii, AA1 and AV1, were isolated from freshwater and Eicchornia crassipes roots, respectively. Here, we report their draft genome sequences, which are ~3.6 Mb and have 51% G+C content. The predicted coding sequences (3,259 for strain AA1 and 3,345 for strain AV1) encode beta-lactamases, transpeptidases, and penicillin-binding proteins.
June 15, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28600898/investigation-of-acid-phosphatase-variants-for-the-synthesis-of-phosphate-monoesters
#10
Gábor Tasnádi, Michaela Zechner, Mélanie Hall, Kai Baldenius, Klaus Ditrich, Kurt Faber
The major drawback of using phosphatases for transphosphorylation reactions lies in product depletion caused by the natural hydrolytic activity of the enzymes. Variants of PhoC-Mm from Morganella morganii and NSAP-Eb from Escherichia blattae were studied for their ability to maintain a high product level in the transphosphorylation of various primary alcohols. A single amino acid exchange delivered phosphatase variant PhoC-Mm G92D, which was able to catalyze the phosphorylation of primary alcohols without any major hydrolysis of the formed phosphate esters...
June 10, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28600633/igm-mediated-autoimmune-responses-to-oxidative-specific-epitopes-but-not-nitrosylated-adducts-are-significantly-decreased-in-pregnancy-association-with-bacterial-translocation-perinatal-and-lifetime-major-depression-and-the-tryptophan-catabolite-trycat-pathway
#11
Chutima Roomruangwong, Buranee Kanchanatawan, Sunee Sirivichayakul, George Anderson, André F Carvalho, Sebastien Duleu, Michel Geffard, Michael Maes
Immunoglubulin (Ig)M responses directed to oxidative specific epitopes (OSEs) and nitric oxide (NO)-adducts are significantly associated with major depression and physio-somatic symptoms. End of term serum IgM responses to OSEs and NO-adducts were assayed in pregnant women with (n = 24) and without prenatal depression (n = 25) as well as in 24 non-pregnant women. Associations of IgM/IgA responses to Gram-negative gut commensal bacteria (leaky gut index) and IgA/IgM responses to tryptophan catabolites (TRYCATs) were analyzed...
June 9, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28586429/hafnia-an-enterobacterial-genus-naturally-resistant-to-colistin-revealed-by-three-susceptibility-testing-methods
#12
Aurélie Jayol, Marion Saly, Patrice Nordmann, Armelle Ménard, Laurent Poirel, Véronique Dubois
Objectives: To determine the susceptibility to colistin of Hafnia alvei and Hafnia paralvei , and to compare methods for colistin resistance detection in the Hafnia genus. Methods: A collection of 25 Hafnia isolates was studied. Species were identified by using 16S rRNA gene sequencing with subsequent phylogeny analysis. Susceptibility to colistin was determined using the broth microdilution (BMD) reference method, the Phoenix automated system, the Rapid Polymyxin NP test, the Etest system and the disc diffusion method...
June 6, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28572256/mutational-signatures-in-breast-cancer-the-problem-at-the-dna-level
#13
EDITORIAL
Serena Nik-Zainal, Sandro Morganella
A breast cancer genome is a record of the historic mutagenic activity that has occurred throughout the development of the tumor. Indeed, every mutation may be informative. Although driver mutations were the main focus of cancer research for a long time, passenger mutational signatures, the imprints of DNA damage and DNA repair processes that have been operative during tumorigenesis, are also biologically illuminating. This review is a chronicle of how the concept of mutational signatures arose and brings the reader up-to-date on this field, particularly in breast cancer...
June 1, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28561169/-adequate-antibiotic-prophylaxis-and-therapy-of-periprosthetic-infections-of-the-breast-review-of-468-consecutive-implant-removals
#14
Jörn Andreas Lohmeyer, Mai Alawadi, Philipp Alexander Bergmann, Daniel Schmauss, Klaus Stephan Wittig
Background Periprosthetic infections are feared complications in aesthetic as well as in reconstructive breast surgery. The purpose of our study was to evaluate our institution's specific culture data and to identify most common organisms and suitable antibiotics for prophylaxis and first line treatment in implant-based breast surgery. Patients and Methods We analyzed all patients with a change or removal of breast implants in the period from 01.01.2012 to 31.12.2015 retrospectively. Based on the medical records, the surgical indications were identified and specifically analyzed for signs of infection and capsular fibrosis...
April 2017: Handchirurgie, Mikrochirurgie, Plastische Chirurgie
https://www.readbyqxmd.com/read/28559163/a-cost-saving-strategy-for-processing-isolated-uropathogens-in-community-acquired-urinary-tract-infections
#15
Juan Manuel Sánchez-Calvo, José Luís de Francisco, Eva Torres-Martos, Juan Carlos Alados Arboledas, Maria Dolores López Prieto
Outpatient urine samples are among the most commonly processed in a microbiology laboratory, which involves a high economic burden. The aim of this study was compare cost and efficiency to process uropathogens between MicroScan system (2010-2011) versus a chromogenic medium and the disk diffusion method (2013-2014). In the first period, a total 9918 bacterial populations were isolated from urine samples. Annual estimated costs during 2010 and 2011 for processing were EUR 53,818 and EUR 57,306, respectively (EUR 111,124 total)...
May 27, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28555403/dissemination-of-bla-oxa-370-gene-among-several-enterobacteriaceae-species-in-brazil
#16
C M Magagnin, F P Rozales, L Antochevis, L S Nunes, A S Martins, A L Barth, J M Sampaio, A P Zavascki
OXA-370 is a recently described OXA-48 variant that has only been described in a few Enterobacter spp. and Klebsiella pneumoniae isolates. The purpose of this study is to assess the prevalence of OXA-370-producing isolates in carbapenem-nonsusceptible Enterobacteriaceae recovered from 28 hospitals from Brazil. Real-time PCR was used to determine the presence of bla NDM-1, bla KPC-2, bla VIM-type, bla GES-type, bla OXA-48-like, and bla IMP-type genes. A total of 4,451 Enterobacteriaceae were screened. The gene bla OXA-48-like was detected in 74 (2...
May 30, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28458670/gut-microbiota-species-can-provoke-both-inflammatory-and-tolerogenic-immune-responses-in-human-dendritic-cells-mediated-by-retinoic-acid-receptor-alpha-ligation
#17
Krisztian Bene, Zsofia Varga, Viktor O Petrov, Nadiya Boyko, Eva Rajnavolgyi
Dendritic cells are considered as the main coordinators of both mucosal and systemic immune responses, thus playing a determining role in shaping the outcome of effector cell responses. However, it is still uncovered how primary human monocyte-derived DC (moDC) populations drive the polarization of helper T (Th) cells in the presence of commensal bacteria harboring unique immunomodulatory properties. Furthermore, the individual members of the gut microbiota have the potential to modulate the outcome of immune responses and shape the immunogenicity of differentiating moDCs via the activation of retinoic acid receptor alpha (RARα)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28446177/the-susceptibility-to-fosfomycin-of-gram-negative-bacteria-isolates-from-urinary-tract-infection-in-the-czech-republic-data-from-a-unicentric-study
#18
Miroslav Fajfr, Miroslav Louda, Pavla Paterová, Lenka Ryšková, Jaroslav Pacovský, Josef Košina, Helena Žemličková, Miloš Broďák
BACKGROUND: Against a background of rapid increase of β-lactamase-producing or multi-resistant pathogenic bacteria and the resulting lack of effective antibiotic treatment, some older antibiotics have been tested for new therapeutic uses. One of these is fosfomycin, to which according to studies these resistant bacteria are very sensitive. Our study was designed because there is no data on the fosfomycin susceptibility rate in the Czech Republic. METHOD: In this study from January 2013 to June 2014 3295 unique isolates of Gram-negative bacteria which had caused urinary tract infections were examined...
April 26, 2017: BMC Urology
https://www.readbyqxmd.com/read/28435889/multidrug-resistance-salmonella-genomic-island-1-in-a-morganella-morganii-subsp-morganii-human-clinical-isolate-from-france
#19
Eliette Schultz, Olivier Barraud, Jean-Yves Madec, Marisa Haenni, Axel Cloeckaert, Marie-Cécile Ploy, Benoît Doublet
Salmonella genomic island 1 (SGI1) is a multidrug resistance integrative mobilizable element that harbors a great diversity of antimicrobial resistance gene clusters described in numerous Salmonella enterica serovars and also in Proteus mirabilis. A serious threat to public health was revealed in the recent description in P. mirabilis of a SGI1-derivative multidrug resistance island named PGI1 (Proteus genomic island 1) carrying extended-spectrum-β-lactamase (ESBL) and metallo-β-lactamase resistance genes, blaVEB-6 and blaNDM-1, respectively...
March 2017: MSphere
https://www.readbyqxmd.com/read/28404984/giant-iliopsoas-abscess-caused-by-morganella-morganii
#20
Mikio Nakajima, Masamitsu Shirokawa, Yasuhiko Miyakuni, Tomotsugu Nakano, Hideaki Goto
BACKGROUND While uncommon, iliopsoas abscesses can become the underlying cause of a fever of unknown origin. Even in such cases, it is considered rare for an iliopsoas abscess to extend into the subcutaneous space. CASE REPORT A 74-year-old woman with a history of schizophrenia was referred to our hospital with a high-grade fever. The patient was unaware of her febrile status prior to admission. There was no previous hospital admission. Examination revealed a non-tender mass in the lower right back that the patient had been aware of for approximately 1 month...
April 13, 2017: American Journal of Case Reports
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