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Nandini Chatterjee, Chandan Chatterjee, Sinjan Ghosh, Mainak Mukhopadhyay, Ramkrishna Brahmachari, Kartik Patar
BACKGROUND: Indiscriminate use of antibiotics in urinary tract infections have led to emergence of 'superbugs' worldwide. Periodical review of antibiograms is of utmost importance for optimum patient benefit. OBJECTIVE: To identify the spectrum of organisms responsible for urinary tract infection and evaluate the pattern of antibiotic sensitivity of the organisms. METHODS: Urine samples were collected from all consecutive patients getting admitted in the medicine indoor irrespective of symptomatology...
April 2016: Journal of the Association of Physicians of India
Mahmoud A Mahmoud, Mohamed Abdelsalam, Olfat A Mahdy, Hala M F El Miniawy, Zakia A M Ahmed, Ahmed H Osman, Hussein M H Mohamed, A M Khattab, M A Zaki Ewiss
This paper is a part of a multi-disciplinary research "Application of Decentralized On-Site Water Treatment System in Egypt for Use in Agriculture and Producing Safe Fish and Animal Proteins". The project aimed to investigate the environmental impact of implementing sewage water before and after treatment using the effluent of the on-site decentralized Japanese' Johkasou system, in agriculture and producing fish protein. The aim is to establish such system in Egypt to strengthen the sanitary conditions of water resources...
October 6, 2016: Environmental Pollution
Daniela Jones-Dias, Vera Manageiro, Eugénia Ferreira, Paula Barreiro, Luís Vieira, Inês B Moura, Manuela Caniça
The spread of antibiotic resistant bacteria throughout the food chain constitutes a public health concern. To understand the contribution of fresh produce in shaping antibiotic resistance bacteria and integron prevalence in the food chain, 333 antibiotic resistance Gram negative isolates were collected from organic and conventionally produced fruits (pears, apples, and strawberries) and vegetables (lettuces, tomatoes, and carrots). Although low levels of resistance have been detected, the bacterial genera identified in the assessed fresh produce are often described not only as environmental, but mostly as commensals and opportunistic pathogens...
2016: Frontiers in Microbiology
Kristin Bjornsdottir-Butler, Maria Sanchez Leon, Ronald A Benner
Histamine-producing bacteria are responsible for scombrotoxin (histamine) fish poisoning, a leading cause of fish poisoning in the United States. We report here the first draft genomes of three histamine-producing Morganella psychrotolerans strains, isolated from tuna and mahi-mahi.
2016: Genome Announcements
H Y Su, M J Wang, Y H Li, C N Tang, M J Tsai
OBJECTIVES: To investigate the clinical predictors and the aetiologies for surgery in patients with Naja atra (Taiwan or Chinese cobra) envenomation. METHODS: This case series was conducted in the only tertiary care centre in eastern Taiwan. Patients who presented to the emergency department with Naja atra bite between January 2008 and September 2014 were included. Clinical information was collected and compared between surgical and non-surgical patients. RESULTS: A total of 28 patients with Naja atra envenomation presented to the emergency department during the study period...
October 2016: Hong Kong Medical Journal, Xianggang Yi Xue za Zhi
Asmita De, Hirak Jyoti Raj, Prasanta Kumar Maiti
Morganella morganii is a member of Enterobacteriaceae family, whose natural habitat is the human gastrointestinal tract. It rarely causes infection alone and is generally encountered in immunosuppressed patients. Osteoarticular pathologies are not commonly observed with Morganella morganii and infections by it have high mortality rate. Biofilm colonization is a causative factor behind the chronicity and/or refractoriness of certain infections. Biofilms colonize on inert medical devices, prosthesis, fibrosed tissues, sinus tracts as well as dead bones as in case of chronic osteomyelitis...
June 2016: Journal of Clinical and Diagnostic Research: JCDR
M Jonas, A Jóźwik, D Kawecki, M Durlik, L Pączek, G Młynarczyk, A Chmura
BACKGROUND: One of the most important problem in kidney transplantation is risk of the urinary anastomosis stricture. In uncertain cases the use of a double-J (or JJ) catheter is a standard solution. In case of urinary leak or fistula after the reanastomosis operation, transuretheral JJ implantation is used. A number of patients after JJ use present urinary infection. METHODS: Between 2012 and 2014, 283 kidney transplantations were performed in our center. In 77 cases (27...
June 2016: Transplantation Proceedings
Jialiang Zhu, Haifeng Li, Li Feng, Min Yang, Ronggong Yang, Lin Yang, Li Li, Ruoyan Li, Minshan Liu, Shuxun Hou, Yuehua Ke, Wenfeng Li, Fan Bai
A case of chronic osteomyelitis probably caused by Morganella morganii, occurring over a period of 30 years, is reported. The organism was identified through a combination of sample culture, direct sequencing, and 16S RNA gene amplicon sequencing. Further whole-genome sequencing and population structure analysis of the isolates from the patient showed the bacterial population to be highly diverse. This case provides a valuable example of a long-term infection caused by an opportunistic pathogen, M. morganii, with high diversity, which might evolve during replication within the host...
September 2016: International Journal of Infectious Diseases: IJID
Yvonne Pfeifer, Angelina Trifonova, Michael Pietsch, Magdalena Brunner, Iva Todorova, Ivanka Gergova, Gottfried Wilharm, Guido Werner, Encho Savov
We characterized 72 isolates with reduced susceptibility to carbapenems (50 Acinetobacter spp., 13 Proteus mirabilis, five Escherichia coli, one Morganella morganii, one Enterobacter cloacae, one Providencia rettgeri, and one Pseudomonas aeruginosa) from a hospital in Sofia, Bulgaria. Different β-lactamase genes were identified by polymerase chain reaction and sequencing. Bacterial strain typing was performed by enzymatic macrorestriction and pulsed-field gel electrophoresis (PFGE) typing as well as multilocus sequence typing for selected isolates...
July 26, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Hamed Ebrahimzadeh Leylabadlo, Hossein Samadi Kafil, Mehdi Yousefi, Mohammad Aghazadeh, Mohammad Asgharzadeh
Organ transplant recipients under immunosuppressive therapy have a highly increased risk of acquiring unusual opportunistic infections. Diagnosis of the etiology of infection may be difficult in clinical manifestations, which need further histological and biological investigations. Here in we report, for the 1(st) time in the Iran, a Morganella morganii isolate harboring blaVIM, blaCTX-M, and blaSHV genes after kidney transplantation with persistent urinary infections.
July 2016: Journal of Natural Science, Biology, and Medicine
Hui Liu, Junmin Zhu, Qiwen Hu, Xiancai Rao
Morganella morganii belongs to the tribe Proteeae of the Enterobacteriaceae family. This species is considered as an unusual opportunistic pathogen that mainly causes post-operative wound and urinary tract infections. However, certain clinical M. morganii isolates present resistance to multiple antibiotics by carrying various resistant genes (such as blaNDM-1, and qnrD1), thereby posing a serious challenge for clinical infection control. Moreover, virulence evolution makes M. morganii an important pathogen...
September 2016: International Journal of Infectious Diseases: IJID
Carlos A Hinojosa, Estefania Boyer-Duck, Javier E Anaya-Ayala, Ana Nunez-Salgado, Hugo Laparra-Escareno, Adriana Torres-Machorro, Rene Lizola
Infections in feet of patients with diabetes mellitus (DM) is common, complex and costly. The aim of this study to investigate the isolated microorganisms in infected diabetic foot ulcers, and the impact of these infectious agents in limb loss in a tertiary medical center in Mexico City. We conducted a retrospective review in diabetic patients with infected foot ulcers from 1997 to 2014. Diabetic foot was defined according to the World Health Organization (WHO), the bacteriology of wound cultures and the impact of microorganisms in limb loss (major amputation) was studied...
July 7, 2016: Wound Repair and Regeneration
Dorina Timofte, Carmen Valentina Panzaru, Iuliana Elena Maciuca, Maria Dan, Anca Delia Mare, Adrian Man, Felicia Toma
We report the findings of an active surveillance scheme for detection of asymptomatic carriers with carbapenemase-producing Gram-negative bacteria (CP-GNB) in Romanian hospitals. During a pilot study from December 2014 to May 2015, faecal cultures were screened in three hospitals (two large, one medium-size) for patients newly admitted to selected wards or inpatients transferred from other wards to an intensive-care unit. The study revealed a high prevalence of CP-GNB detected in 22/27 and 28/38 of the carbapenem non-susceptible isolates from Hospitals 1 and 3, respectively...
June 23, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
D G Marshall, T A Jackson, C R Unelius, S L Wee, S D Young, R J Townsend, D M Suckling
Costelytra zealandica (Coleoptera: Scarabeidae) is a univoltine endemic species that has colonised and become a major pest of introduced clover and ryegrass pastures that form about half of the land area of New Zealand. Female beetles were previously shown to use phenol as their sex pheromone produced by symbiotic bacteria in the accessory or colleterial gland. In this study, production of phenol was confirmed from the female beetles, while bacteria were isolated from the gland and tested for attractiveness towards grass grub males in traps in the field...
August 2016: Die Naturwissenschaften
Abbas S Al-Muhanna, Sddiq Al-Muhanna, Maytham A Alzuhairi
BACKGROUND: Resistance to beta-lactam antibiotics has become more common in Morganella morganii, which can cause of outbreaks of bacteremia and septicemia in postoperative patients. OBJECTIVE: Investigate drug susceptibility of M morganii, identify the gene responsible for extended-spectrum beta-lactamase (ESBL) production and explore treatment options. DESIGN: Descriptive study. SETTING: Hospitals in An Najaf, Iraq...
May 2016: Annals of Saudi Medicine
Jianming Cao, Meimei Li, Chunquan Xu, Tieli Zhou, Jia Du, Yao Sun, Ling Qin, Jiru Xu
BACKGROUND: Proteeae isolates displaying multidrug-resistance (MDR) are the second most common causes of hospital-associated infections. The aim of this study was to screen class 1-3 integrons and plasmid-mediated quinolone resistance (PMQR) genes in Proteeae isolates from the First Affiliated Hospital of the Wenzhou Medical University. MATERIALS AND METHODS: 176 Proteeae isolates were collected from clinical specimens of inpatients between January 2011 and December 2013...
May 25, 2016: Chemotherapy
E Tajeddin, S J Sherafat, M R S Majidi, M Alebouyeh, A H M Alizadeh, M R Zali
Bacterial infection is considered a predisposing factor for disorders of the biliary tract. This study aimed to determine the diversity of bacterial communities in bile samples and their involvement in the occurrence of biliary tract diseases. A total of 102 bile samples were collected during endoscopic retrograde cholangiopancreatography (ERCP). Characterization of bacteria was done using culture and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) methods. Antimicrobial susceptibility of the isolates was determined based on the Clinical and Laboratory Standards Institute (CLSI) guidelines and identity of the nucleotide sequences of differentiated bands from the DGGE gels was determined based on GenBank data...
August 2016: European Journal of Clinical Microbiology & Infectious Diseases
V P Kartik, H N Jinal, N Amaresan
The cadmium (Cd) resistant bacteria were isolated from soils of Damanganga river, Vapi, and identified 11 potential Cd resistant bacteria based on 16S rDNA sequences. The Cd resistant bacteria belonged to four different genera: Providencia spp., Morganella sp., Stenotrophomonas sp., and Bacillus spp. The assessment of plant growth-promoting (PGP) parameters revealed that the Cd tolerant bacteria showed one or more PGP properties. Further, a pot experiment was conducted to elucidate the effects of Cd resistant bacteria on the plant growth and the uptake of Cd by Sesbania bispinosa...
November 2016: International Journal of Phytoremediation
Gerardo Vázquez-Marrufo, Jesús Andrei Rosales-Castillo, Virginia A Robinson-Fuentes, Iliana Tafolla-Muñoz, Nohemí Carreras-Villaseñor, Ma Soledad Vázquez-Garcidueñas
ETEC is the most common cause of diarrhea in children under 5 years from developing world. We used the sequencing of the 16S rRNA gene, the Biolog® system, and the Amplified Ribosomal DNA Restriction Analysis (ARDRA), to identify 69 Enterobacteria isolated from feces of healthy children up to 12 years old, and 54 Enterobacteria isolated from stool samples obtained from children up to 5 years old with diarrhea, from Morelia, Michoacán, Mexico. In the diarrheic group, 18 isolates belong to the Enterotoxigenic pathotype, 17 have the ST gene and one has both LT and ST genes...
May 9, 2016: Japanese Journal of Infectious Diseases
Sandro Morganella, Ludmil B Alexandrov, Dominik Glodzik, Xueqing Zou, Helen Davies, Johan Staaf, Anieta M Sieuwerts, Arie B Brinkman, Sancha Martin, Manasa Ramakrishna, Adam Butler, Hyung-Yong Kim, Åke Borg, Christos Sotiriou, P Andrew Futreal, Peter J Campbell, Paul N Span, Steven Van Laere, Sunil R Lakhani, Jorunn E Eyfjord, Alastair M Thompson, Hendrik G Stunnenberg, Marc J van de Vijver, John W M Martens, Anne-Lise Børresen-Dale, Andrea L Richardson, Gu Kong, Gilles Thomas, Julian Sale, Cristina Rada, Michael R Stratton, Ewan Birney, Serena Nik-Zainal
Somatic mutations in human cancers show unevenness in genomic distribution that correlate with aspects of genome structure and function. These mutations are, however, generated by multiple mutational processes operating through the cellular lineage between the fertilized egg and the cancer cell, each composed of specific DNA damage and repair components and leaving its own characteristic mutational signature on the genome. Using somatic mutation catalogues from 560 breast cancer whole-genome sequences, here we show that each of 12 base substitution, 2 insertion/deletion (indel) and 6 rearrangement mutational signatures present in breast tissue, exhibit distinct relationships with genomic features relating to transcription, DNA replication and chromatin organization...
2016: Nature Communications
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