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https://www.readbyqxmd.com/read/28814259/may-gender-or-ethnicity-affect-delta-over-baseline-values-obtained-by-13-c-urea-breath-test
#1
Carmine Petruzziello, Dario Sinatti, Maria Gnarra, Alessio Migneco, Valentina Tesori, Cristina Graziani, Antonio Gasbarrini, Francesco Franceschi, Veronica Ojetti
INTRODUCTION: 13C-urea breath test (UBT) is a non-invasive test for detecting active H.pylori infection. Previous studies showed a correlation of delta over baseline (DOB) values with bacterial load, mucosal inflammation and successful eradication. Gender has been shown to affect DOB in children. Aim of our study was to verify whether gender or ethnicity affects DOB in adults. PATIENTS AND METHODS: We retrospectively analyzed data of 2922 patients (1024M/1898F mean age 47±15 years) that underwent UBT in our outpatient unit, from October 2015 to October 2016...
August 16, 2017: Reviews on Recent Clinical Trials
https://www.readbyqxmd.com/read/28813027/sulfonamide-linked-ciprofloxacin-sulfadiazine-and-amantadine-derivatives-as-a-novel-class-of-inhibitors-of-jack-bean-urease-synthesis-kinetic-mechanism-and-molecular-docking
#2
Pervaiz Ali Channar, Aamer Saeed, Fernando Albericio, Fayaz Ali Larik, Qamar Abbas, Mubashir Hassan, Hussain Raza, Sung-Yum Seo
Sulfonamide derivatives serve as an important building blocks in the drug design discovery and development (4D) process. Ciprofloxacin-, sulfadiazine- and amantadine-based sulfonamides were synthesized as potent inhibitors of jack bean urease and free radical scavengers. Molecular diversity was explored and electronic factors were also examined. All 24 synthesized compounds exhibited excellent potential against urease enzyme. Compound 3e (IC50 = 0.081 ± 0.003 µM), 6a (IC50 = 0.0022 ± 0.0002 µM), 9e (IC50 = 0...
August 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28811799/diversity-physiochemical-and-phylogenetic-analyses-of-bacteria-isolated-from-various-drinking-water-sources
#3
Neveen H Eid, Huda A Al Doghaither, Taha A Kumosani, Munazza Gull
OBJECTIVE: To evaluate the indigenous bacterial strains of drinking water from the most commercial water types including bottled and filtered water that are currently used in Saudi Arabia. METHODS: Thirty randomly selected commercial brands of bottled water were purchased from Saudi local markets. Moreover, samples from tap water and filtered water were collected in sterilized glass bottles and stored at 4°C. Biochemical analyses including pH, temperature, lactose fermentation test (LAC), indole test (IND), methyl red test (MR), Voges-Proskauer test (VP), urease test (URE), catalase test (CAT), aerobic and anaerobic test (Ae/An) were measured...
May 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28810218/effect-of-cold-adapted-microbial-agent-inoculation-on-enzyme-activities-during-composting-start-up-at-low-temperature
#4
Qinghong Sun, Di Wu, Zhechao Zhang, Yue Zhao, Xinyu Xie, Junqiu Wu, Qian Lu, Zimin Wei
In order to put forward a method to promote composting start-up at low ambient temperature, the cold-adapted microbial agent (CAMA) was inoculated in chicken manure (CM), and compared the enzymes activities, including urease, proteases, β-glucosidase and invertase, with no CAMA group (CK). In this study, the temperature of CM reached 50°C in 53h, but it in CK was only around 30°C during the composting process. Moreover, the enzymes exhibited higher activity in CM than CK, indicating the effectiveness of CAMA...
August 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28809946/structure-function-analyses-of-metal-binding-sites-of-hypa-reveal-residues-important-for-hydrogenase-maturation-in-helicobacter-pylori
#5
Faith C Blum, Heidi Q Hu, Stephanie L Servetas, Stéphane L Benoit, Robert J Maier, Michael J Maroney, D Scott Merrell
The nickel-containing enzymes of Helicobacter pylori, urease and hydrogenase, are essential for efficient colonization in the human stomach. The insertion of nickel into urease and hydrogenase is mediated by the accessory protein HypA. HypA contains an N-terminal nickel-binding site and a dynamic structural zinc-binding site. The coordination of nickel and zinc within HypA is known to be critical for urease maturation and activity. Herein, we test the hydrogenase activity of a panel of H. pylori mutant strains containing point mutations within the nickel- and zinc-binding sites...
2017: PloS One
https://www.readbyqxmd.com/read/28806558/nanoscale-zero-valent-iron-coated-with-rhamnolipid-as-an-effective-stabilizer-for-immobilization-of-cd-and-pb-in-river-sediments
#6
Wenjing Xue, Danlian Huang, Guangming Zeng, Jia Wan, Chen Zhang, Rui Xu, Min Cheng, Rui Deng
Nanoremediation strategies applied to contaminated river sediments can induce changes in the mobility and bioavailability of metals with potential consequences on ecosystem health. In this study, the performance of rhamnolipid (RL) coated nanoscale zero-valent iron (NZVI) in immobilizing cadmium (Cd) and lead (Pb) from contaminated river sediments was investigated. We demonstrated that RNZVI was effective in transforming labile Cd and Pb to stable fraction (the maximum residual percentage of Cd and Pb increased by 56...
June 15, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28805287/nongenetically-modified-lactococcus-lactis-adjuvanted-vaccination-enhanced-innate-immunity-against-helicobacter-pylori
#7
Wei Liu, Zhoulin Tan, Hai Liu, Zhiqin Zeng, Shuanghui Luo, Huimin Yang, Lufeng Zheng, Tao Xi, Yingying Xing
BACKGROUND: Gram-positive enhancer matrix particles (GEM) produced by Lactococcus lactis can enhance vaccine-induced immune response. However, the mechanism under which this adjuvant mounts the efficacy of orally administered vaccines remains unexplored. MATERIALS AND METHODS: We used a prophylactic mice model to investigate the mechanism of GEM-adjuvanted vaccination. Helicobacter pylori urease-specific antibody response was monitored and detected in murine serum by ELISA...
August 14, 2017: Helicobacter
https://www.readbyqxmd.com/read/28802269/removal-of-soluble-strontium-into-biogenic-carbonate-minerals-from-a-highly-saline-solution-using-halophilic-bacterium-bacillus-sp-tk2d
#8
Takumi Horiike, Yuma Dotsuta, Yuriko Nakano, Asumi Ochiai, Satoshi Utsunomiya, Toshihiko Ohnuki, Mitsuo Yamashita
Radioactive strontium ((90)Sr) leaked into saline environments, including the ocean, from the Fukushima Daiichi Nuclear Power Plant after a nuclear accident. Since the removal of (90)Sr using general adsorbents (e.g., zeolite) is not efficient at high salinity, a suitable alternative immobilization method is necessary. Therefore, we incorporated soluble Sr into biogenic carbonate minerals generated by urease-producing microorganisms from a saline solution. An isolate, Bacillus sp. strain TK2d, from marine sediment removed > 99% Sr after contact for four days in a saline solution (1...
August 11, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28794636/helicobacter-pylori-infection-and-severity-of-coronary-atherosclerosis-in-patients-with-chronic-coronary-artery-disease
#9
Anita Jukic, Dorotea Bozic, Dusko Kardum, Tina Becic, Bruno Luksic, Mislav Vrsalovic, Marko Ljubkovic, Damir Fabijanic
AIM: Controversy exists concerning the relation between Helicobacter pylori (HP) infection and coronary artery disease (CAD). We aimed to examine the relationship between HP infection and severity of coronary atherosclerosis in patients with chronic CAD. PATIENTS AND METHODS: A total of 150 patients (109 [73%] men; mean age 62.61±10.23 years) scheduled for coronary artery bypass grafting surgery were consecutively enrolled in the cross-sectional study. According to rapid urease test and/or gastric biopsy samples stained with hematoxylin and eosin and according to Giemsa, patients were classified as HP positive (n=87; 58%) or HP negative (n=63; 42%)...
2017: Therapeutics and Clinical Risk Management
https://www.readbyqxmd.com/read/28792967/aminophosphinates-against-helicobacter-pylori-ureolysis-biochemical-and-whole-cell-inhibition-characteristics
#10
Katarzyna Macegoniuk, Ewa Grela, Monika Biernat, Mateusz Psurski, Grażyna Gościniak, Anna Dziełak, Artur Mucha, Joanna Wietrzyk, Łukasz Berlicki, Agnieszka Grabowiecka
Urease is an important virulence factor from Helicobacter pylori that enables bacterial colonization of human gastric mucosa. Specific inhibition of urease activity can be regarded as a promising adjuvant strategy for eradication of this pathogen. A group of organophosphorus inhibitors of urease, namely, aminophosphinic acid and aminophosphonic acid derivatives, were evaluated in vitro against H. pylori urease. The kinetic characteristics of recombinant enzyme activity demonstrated a competitive reversible mode of inhibition with Ki values ranging from 0...
2017: PloS One
https://www.readbyqxmd.com/read/28791813/an-evaluation-of-selected-oral-health-indicators-and-cariogenic-bacteria-titer-in-patients-with-helicobacter-pylori
#11
Jakub Urban, Rafał Koszowski, Anna Płachetka, Andrzej Wiczkowski
BACKGROUND: Studies based on polymerase chain reaction (PCR) techniques indicate that Helicobacter pylori can be constantly or temporarily present in the oral cavity in virulent or non-virulent form. Streptococcus mutans exerts a strong inhibitory effect on H. pylori. OBJECTIVES: The aim of the present study was to investigate the prevalence and virulence of H. pylori in the oral cavity and the correlation of these factors with oral health and cariogenic bacteria titer...
May 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28791504/the-effects-of-grazing-intensity-on-soil-processes-in-a-mediterranean-protected-area
#12
Evaggelia Panayiotou, Maria Dimou, Nikolaos Monokrousos
We investigated the temporal and among-site differentiation of soil functionality properties in fields under different grazing intensities (heavy and light) and compared them to those found in their adjacent hedgerows, consisting either of wooden shrubs (Rubus canescens) or of high trees (Populus sp.), during the cold and humid seasons of the year. We hypothesized that greater intensity of grazing would result in higher degradation of the soil system. The grazing factor had a significant effect on soil organic C and N, microbial biomass C, microbial biomass N, microbial activity, and β-glucosidase, while acid phosphatase and urease activity were not found to differ significantly among the management systems...
August 8, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28791039/impact-of-transgenic-brassica-napus-harboring-the-antifungal-synthetic-chitinase-nic-gene-on-rhizosphere-microbial-diversity-and-enzyme-activities
#13
Mohammad S Khan, Syed U Sadat, Asad Jan, Iqbal Munir
Transgenic Brassica napus harboring the synthetic chitinase (NiC) gene exhibits broad-spectrum antifungal resistance. As the rhizosphere microorganisms play an important role in element cycling and nutrient transformation, therefore, biosafety assessment of NiC containing transgenic plants on soil ecosystem is a regulatory requirement. The current study is designed to evaluate the impact of NiC gene on the rhizosphere enzyme activities and microbial community structure. The transgenic lines with the synthetic chitinase gene (NiC) showed resistance to Alternaria brassicicola, a common disease causing fungal pathogen...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28772021/self-regulated-and-temporal-control-of-a-breathing-microgel-mediated-by-enzymatic-reaction
#14
Jan van Hest, Hailong Che, Bastiaan Buddingh'
Naturally occurring systems have the ability to self-regulate, which plays a key role in their structural and functional adaption. The autonomous behaviour in living systems is biocatalytically controlled by the continuous consumption of energy to remain in a non-equilibrium condition. In this work, we show the construction of a self-regulated "breathing" microgel that uses chemical fuels to keep the system in the out-of-equilibrium state. The enzyme urease is utilized to programme a feedback-induced pH change which in turn tunes the size switch and fluorescence intensity of the microgel...
August 3, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28764169/histomorphological-spectrum-of-duodenal-pathology-in-functional-dyspepsia-patients
#15
Anita Aujenath Chaudhari, Sharada Raju Rane, Meenal Vitthal Jadhav
INTRODUCTION: Functional Dyspepsia (FD) is one of the most common causes of gastrointestinal symptoms aetiology of which is poorly understood. AIM: To study duodenal histomorphological features and their relationship with Helicobacter pylori (H Pylori) infection in patients of FD. MATERIALS AND METHODS: This case control study included 50 cases of FD patients selected according to Rome III criteria and 30 age and sex matched controls. These were subjected to oesophago-gastro-duodenoscopy, rapid urease test for detection of H...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28764161/providencia-rettgeri-an-emerging-nosocomial-uropathogen-in-an-indwelling-urinary-catheterised-patient
#16
Siddharth Sagar, Nagalakshmi Narasimhaswamy, Jessica D'Souza
Providencia rettgeri (P. rettgeri) is an ubiquitous organism but is seldom associated with human disease. We report the isolation of P. rettgeri from the urine sample of a 39-year-old male patient on prolonged Foley's catheterisation following a severe head injury. Identification of this organism was done by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF) based systems. P. rettgeri is an emerging pathogen among long term catheterised patients. It reflects its ability to form biofilm on the surface of the indwelling catheter as well as the inherent urease producing property of the pathogen in question as a possible mechanism of pathogenesis...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28761331/antiglycation-radical-scavenging-and-semicarbazide-sensitive-amine-oxidase-inhibitory-activities-of-acetohydroxamic-acid-in-vitro
#17
Yuh-Hwa Liu, Yeh-Lin Lu, Der-Zen Liu, Wen-Chi Hou
Advanced glycation endproducts (AGEs) can promote intracellular reactive oxygen species production, and the levels of AGEs are highly correlated with cardiovascular disease and diabetes complications. Acetohydroxamic acid (acetH) is a bacterial urease inhibitor drug used to treat kidney stones and infections in the urinary tract, and hydroxyurea (HU) is a drug used for antineoplasm and sickle cell diseases. Both acetH and HU are hydroxamic acid derivatives. It was found that acetH and HU at 2.5 or 5 mM showed anti-AGE formation by lowering the AGEs' fluorescent intensities and N(ε)-(carboxymethyl)lysine formation in bovine serum albumin/galactose models, and both showed better and significant differences (P<0...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28753459/design-synthesis-in-vitro-evaluation-and-docking-studies-on-dihydropyrimidine-based-urease-inhibitors
#18
Fatima Iftikhar, Yousaf Ali, Farooq Ahmad Kiani, Syed Fahad Hassan, Tabeer Fatima, Ajmal Khan, Basit Niaz, Abbas Hassan, Farzana Latif Ansari, Umer Rashid
In our previous report, we have identified 3,4-dihydropyrimidine scaffold as promising class of urease inhibitor in a structure based virtual screen (SBVS) experiment. In present study, we attempted to optimize the scaffold by varying C-5 substituent. The elongation of the C-5 chain was achieved by the reaction of C-5 ester with hydrazine leading to C-5 carbohydrazides which were further used as building blocks for the synthesis of fifteen new compounds having diverse moieties. A significantly higher in vitro urease inhibitory activity with IC50 values in submicromolar range was observed for semithiocarbazide derivatives (4a-c, 0...
July 19, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28748114/uric-acid-in-plants-and-microorganisms-biological-applications-and-genetics-a-review
#19
REVIEW
Rehab M Hafez, Tahany M Abdel-Rahman, Rasha M Naguib
Uric acid increased accumulation and/or reduced excretion in human bodies is closely related to pathogenesis of gout and hyperuricemia. It is highly affected by the high intake of food rich in purine. Uric acid is present in both higher plants and microorganisms with species dependent concentration. Urate-degrading enzymes are found both in plants and microorganisms but the mechanisms by which plant degrade uric acid was found to be different among them. Higher plants produce various metabolites which could inhibit xanthine oxidase and xanthine oxidoreductase, so prohibit the oxidation of hypoxanthine to xanthine then to uric acid in the purine metabolism...
September 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28747573/the-influence-of-adjuvant-on-ureb-protection-against-helicobacter-pylori-through-the-diversity-of-cd4-t-cell-epitope-repertoire
#20
Bin Li, Hanmei Yuan, Li Chen, Heqiang Sun, Jian Hu, Shanshan Wei, Zhuo Zhao, Quanming Zou, Chao Wu
Adjuvants are widely used to enhance the effects of vaccines against pathogen infections. Interestingly, different adjuvants and vaccination routes usually induce dissimilar immune responses, and can even have completely opposite effects. The mechanism remains unclear. In this study, urease B subunit (UreB), an antigen of Helicobacter pylori (H. pylori) that can induce protective immune responses, was used as a model to vaccinate mice. We investigated the effects of different adjuvants and routes on consequent T cell epitope-specific targeting and protection against H...
July 12, 2017: Oncotarget
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