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https://www.readbyqxmd.com/read/28213182/microbial-nickel-cellular-uptake-and-delivery-to-enzyme-centers
#1
REVIEW
Conor J Zeer-Wanklyn, Deborah B Zamble
Nickel enzymes allow microorganisms to access chemistry that can be vital for survival and virulence. In this review we highlight recent work on several systems that import nickel ions and deliver them to the active sites of these enzymes. Small molecules, in particular l-His and derivatives, may chelate nickel ions before import at TonB-dependent outer-membrane and ABC-type inner-membrane transporters. Inside the cell, nickel ions are used by maturation factors required to produce nickel enzymes such as [NiFe]-hydrogenase, urease and lactate racemase...
February 14, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28207990/carbonic-anhydrase-and-urease-inhibitory-potential-of-various-plant-phenolics-using-in-vitro-and-in-silico-methods
#2
Abdur Rauf, Muslim Raza, Muhammad Saleem, Ufuk Ozgen, Esen Sezen Karaoglan, Gulin Renda, Erhan Palaska, Ilkay Erdogan Orhan
Plant phenolics are known to display many pharmacological activities. In the current study, eight phenolic compounds [e.g. luteolin 5-O-β-glucoside (1), methyl rosmarinate (2), apigenin (3), vicenin 2 (4), lithospermic acid (5), soyasaponin II (6), rubiadin 3-O-β-primeveroside (7), 4(3,4dihydroxybenzoyloxymethyl)phenyl-O-β-D-glucopyranoside (8)] isolated from various plant species were tested at 0.2 mM against carbonic anhydrase-II (CA-II) and urease using microtiter assays. Urease inhibition rate for compounds 1-8 ranged between 5...
February 16, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28198807/paenibacillus-polymyxa-nsy50-suppresses-fusarium-wilt-in-cucumbers-by-regulating-the-rhizospheric-microbial-community
#3
Lu Shi, Nanshan Du, Sheng Shu, Jin Sun, Shuzhan Li, Shirong Guo
Paenibacillus polymyxa (P. polymyxa) NSY50, isolated from vinegar residue substrate, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. The aim of the present study was to evaluate the effects of NSY50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to Fusarium oxysporum. Bacterial and fungal communities were investigated by Illumina sequencing of the 16S rRNA gene and the internal transcribed spacer (ITS) regions (ITS1 and ITS2)...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28197855/soil-microbial-community-responses-to-contamination-with-silver-aluminium-oxide-and-silicon-dioxide-nanoparticles
#4
C F McGee, S Storey, N Clipson, E Doyle
Soil microorganisms are key contributors to nutrient cycling and are essential for the maintenance of healthy soils and sustainable agriculture. Although the antimicrobial effects of a broad range of nanoparticulate substances have been characterised in vitro, little is known about the impact of these compounds on microbial communities in environments such as soil. In this study, the effect of three widely used nanoparticulates (silver, silicon dioxide and aluminium oxide) on bacterial and fungal communities in an agricultural pastureland soil was examined in a microcosm-based experiment using a combination of enzyme analysis, molecular fingerprinting and amplicon sequencing...
February 14, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28197768/expression-of-an-acid-urease-with-urethanase-activity-in-e-coli-and-analysis-of-urease-gene
#5
REVIEW
Xiaofeng Liu, Qian Zhang, Nandi Zhou, Yaping Tian
Urea in alcoholic beverage is a precursor of ethyl carbamate (EC), which is carcinogenic. Enzymatic elimination of urea has attracted much research interest. Acid urease with good tolerance toward ethanol and acid is ideal enzyme for such applications. In the present work, the structural genes of urease from Providencia rettgeri JN-B815, ureABC were efficiently expressed in E. coli BL21(DE3) in an active form (apourease) exhibiting both urease and urethanase (hydrolyze EC) activities. The specific activities of the purified apourease were comparatively low, which were 2...
February 14, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28193587/the-effects-of-rice-straw-biochar-on-indigenous-microbial-community-and-enzymes-activity-in-heavy-metal-contaminated-sediment
#6
Danlian Huang, Linshan Liu, Guangming Zeng, Piao Xu, Chao Huang, Linjing Deng, Rongzhong Wang, Jia Wan
Owning to the potential in carbon sequestration and other environmental benefits, biochar has been widely used for in-situ environmental remediation. Understanding the biological effects of biochar is essential. The goal of this study was to explore the response of indigenous microbes under the stress of different concentrations of biochar. The results showed that biochar could significantly change physicochemical properties, enzymes activity and microbial community composition depending on biochar concentration and incubation time...
January 30, 2017: Chemosphere
https://www.readbyqxmd.com/read/28193167/helicobacter-pylori-prevalence-and-clinical-significance-in-patients-with-quiescent-crohn-s-disease
#7
Adi Lahat, Uri Kopylov, Sandra Neuman, Nina Levhar, Doron Yablecovitch, Benjamin Avidan, Batia Weiss, Shomron Ben-Horin, Rami Eliakim
BACKGROUND: Helicobacter pylori (HP) infection is present in about 50% of the global population, and is associated with chronic gastritis, peptic disease and gastric malignancies. HP prevalence in Crohn's disease (CD) patients was shown to be low compared to the general population, and its influence on disease activity is yet to be determined. Our aims were to determine the prevalence of HP in a selected group of CD patients with quiescent disease, and to assess the influence of its eradication on disease activity and endoscopic and laboratory activity measures...
February 13, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28191542/loose-ends-in-the-eradication-of-helicobacter-pylori-infection
#8
Armando Peixoto, Marco Silva, Rui Gaspar, Rui Morais, Rosa Ramalho, Guilherme Macedo, João Santos-Antunes
The eradication of Helicobacter pylori is essential for prevention and treatment of various conditions associated with this infection. However, its effectiveness is limited and influenced by factors linked to the bacteria and the host. In particular, influence of the biotype, smoking, diabetes mellitus, and previous treatment failure in eradication is understudied. Our center proposed to evaluate these aspects in a real life cohort by applying a questionnaire with demographic and lifestyle variables in patients who consecutively underwent urease breath test after the eradication therapy...
December 1, 2016: Journal of Translational Internal Medicine
https://www.readbyqxmd.com/read/28185986/a-novel-design-of-a-multi-antigenic-multistage-and-multi-epitope-vaccine-against-helicobacter-pylori-an-in-silico-approach
#9
Beatriz Meza, Felipe Ascencio, Arturo Pedro Sierra-Beltrán, Javier Torres, Carlos Angulo
Helicobacter pylori have colonized the gastric mucosa of half of the population worldwide. This bacterium is classified as a definitive type I carcinogen by the World Health Organization and no effective vaccine has been found against it yet. Thus, a logical and rational vaccine design against H. pylori is necessary. Because of its tremendous complexity and elicited immune responses, the vaccine design should considered multiple antigens to enhance immune-protection, involved in the different stages of pathogenesis besides inducing a specific immune response by B- and T-cell multi-epitopes...
February 7, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28179897/comparative-transcriptomics-reveals-discrete-survival-responses-of-s-aureus-and-s-epidermidis-to-sapienic-acid
#10
Josephine C Moran, Jamal A Alorabi, Malcolm J Horsburgh
Staphylococcal colonization of human skin is ubiquitous, with particular species more frequent at different body sites. Whereas Staphylococcus epidermidis can be isolated from the skin of every individual tested, Staphylococcus aureus is isolated from <5% of healthy individuals. The factors that drive staphylococcal speciation and niche selection on skin are incompletely defined. Here we show that S. aureus is inhibited to a greater extent than S. epidermidis by the sebaceous lipid sapienic acid, supporting a role for this skin antimicrobial in selection of skin staphylococci...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28177601/nickel-ligation-of-the-n-terminal-amine-of-hypa-is-required-for-urease-maturation-in-helicobacter-pylori
#11
Heidi Q Hu, Ryan C Johnson, Douglas Scott Merrell, Michael J Maroney
The human pathogen Helicobacter pylori requires nickel for colonization of the acidic environment of the stomach. HypA, a Ni metallochaperone that is typically associated with hydrogenase maturation, is also required for urease maturation and acid survival of H. pylori. There are two proposed Ni site structures for HypA; one is a paramagnetic 6-coordinate site characterized by X-ray absorption spectroscopy (XAS) in unmodified HypA while another is a diamagnetic four-coordinate planar site characterized by solution NMR in an N-terminally modified HypA construct...
February 8, 2017: Biochemistry
https://www.readbyqxmd.com/read/28177288/novel-urease-negative-helicobacter-sp-h-enhydrae-sp-nov-isolated-from-inflamed-gastric-tissue-of-southern-sea-otters
#12
Zeli Shen, Francesca Batac, Anthony Mannion, Melissa A Miller, Vasudevan Bakthavatchalu, Calvin Ho, Sean Manning, Bruce J Paster, James G Fox
A total of 31 sea otters Enhydra lutris nereis found dead or moribund (and then euthanized) were necropsied in California, USA. Stomach biopsies were collected and transected with equal portions frozen or placed in formalin and analyzed histologically and screened for Helicobacter spp. in gastric tissue. Helicobacter spp. were isolated from 9 sea otters (29%); 58% (18 of 31) animals were positive for helicobacter by PCR. The Helicobacter sp. was catalase- and oxidase-positive and urease-negative. By electron microscopy, the Helicobacter sp...
February 8, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28167234/epiberberine-a-natural-protoberberine-alkaloid-inhibits-urease-of-helicobacter-pylori-and-jack-bean-susceptibility-and-mechanism
#13
Lihua Tan, Cailan Li, Hanbin Chen, Zhizhun Mo, Jiangtao Zhou, Yuhong Liu, Zhilin Ma, Yuyao Xu, Xiaobo Yang, Jianhui Xie, Ziren Su
In our previous study, Rhizoma Coptidis extract was found to exert more potent inhibitory effect than its major component berberine towards urease from Helicobacter pylori (HPU) and jack bean (JBU). In continuation of our work, the present study was designed to further comparatively investigate the urease inhibitory activities of five major protoberberine alkaloids in Rhizoma Coptidis, namely berberine, palmatine, coptisine, epiberberine, jateorhizine to identify the bioactive constituent, and illuminate the potential mechanism of action...
February 4, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28149841/effects-of-curcumin-on-helicobacter-pylori-infection
#14
Vaclav Vetvicka, Jana Vetvickova, Rafael Fernandez-Botran
BACKGROUND: Curcumin is a well-established natural molecule with significant biological and pharmaceutical effects. Its effects on Helicobacter pylori (H. pylori) infection have been repeatedly confirmed both in animal and human models. This study directly compared five different samples to evaluate if the effects are general or if they differ among samples. METHODS: Using a mouse model, we studied the effects of curcumin on lipid peroxide (LPO) level, myeloperoxidase (MPO) and urease activity, number of colonized bacteria, levels of anti-H...
December 2016: Annals of Translational Medicine
https://www.readbyqxmd.com/read/28148734/cutting-edge-helicobacter-pylori-induces-nuclear-hypersegmentation-and-subtype-differentiation-of-human-neutrophils-in-vitro
#15
Laura C Whitmore, Megan N Weems, Lee-Ann H Allen
Helicobacter pylori infects the human stomach and causes a spectrum of disease that includes gastritis, peptic ulcers, and gastric adenocarcinoma. A chronic, neutrophil-rich inflammatory response characterizes this infection. It is established that H. pylori stimulates neutrophil chemotaxis and a robust respiratory burst, but other aspects of this interaction are incompletely defined. We demonstrate here that H. pylori induces N1-like subtype differentiation of human neutrophils as indicated by profound nuclear hypersegmentation, a CD62L(dim), CD16(bright), CD11b(bright), CD66b(bright), CD63(bright) surface phenotype, proinflammatory cytokine secretion, and cytotoxicity...
February 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28143992/an-east-asian-type-caga-helicobacter-pylori-infected-patient-with-clinical-manifestation-of-gastric-ulcer
#16
Yudith Annisa Ayu Rezkitha, Muhammad Miftahussurur, Iswan A Nusi, Ummi Maimunah, Pangestu Adi, Yoshio Yamaoka
We reported a male, 72 yo, Chinese ethnic with chief complaint black mushy defecation. Physical examination revealed pale on conjunctival palpebra which confirmed as anemia on complete blood count. Gastroduodenoscopy revealed a 3 mm ulcer at the antrum (Forrest stage III). H. pylori infection was positive based on five different test methods (urinary antibody tests, rapid urease test, culture, histology ad immunohistochemistry). Used polymerase chain reaction-based sequencing, we found the patient infected by CagA producing, East-Asian-type cagA and vacA s1m1-strain...
October 2016: Acta Medica Indonesiana
https://www.readbyqxmd.com/read/28138399/rapid-identification-of-helicobacter-pylori-and-assessment-of-clarithromycin-susceptibility-from-clinical-specimens-using-fish
#17
Ebru Demiray-Gürbüz, Özlem Yılmaz, Asalia Z Olivares, Can Gönen, Sülen Sarıoğlu, Müjde Soytürk, Sait Tümer, Oğuz Altungöz, İlkay Şimşek, Guillermo I Perez Perez
Helicobacter pylori remains one of the most common bacterial infections worldwide. Clarithromycin resistance is the most important cause of H. pylori eradication failures. Effective antibiotic therapies in H. pylori infection must be rapidly adapted to local resistance patterns. We investigated the prevalence of clarithromycin resistance due to mutations in positions 2142 and 2143 of 23SrRNA gene of H. pylori by fluorescence in situ hybridisation (FISH), and compared with culture and antimicrobial susceptibility testing in 234 adult patients with dyspepsia who were enrolled...
January 2017: Journal of Pathology. Clinical Research
https://www.readbyqxmd.com/read/28138371/detection-of-helicobacter-pylori-in-the-bronchoalveolar-lavage-of-patients-with-lung-cancer-using-real-time-pcr
#18
Mitra Samareh-Fekri, Seyed Mehdi Hashemi Bajgani, Ahmad Shafahi, Mahbobeh Asadi-Zarandi, Hamid Mollaie, Arshia Jamali Paghalhe
BACKGROUND: Lung cancer is one of the most common causes of death worldwide. Although smoking and environmental pollutants are the most important risk factors of lung cancer, the role of infectious causes should also be considered in the pathogenesis and progress of lung cancer. OBJECTIVES: This study examined the relationship between Helicobacter pylori and lung cancer through serology, real-time PCR, and urease tests. METHODS: This descriptive cross-sectional study was conducted on 52 adult patients with lung cancer who were selected after having their history taken and being physically examined by a pulmonologist...
November 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/28137214/phytochemical-constituents-ches-and-urease-inhibition-antiproliferative-and-antioxidant-properties-of-elaeagnus-umbellata-thunb
#19
Tevfik Ozen, Semiha Yenigun, Muhammed Altun, Ibrahim Demirtas
Due to the common ethnopharmacological used or scientifically examined biochemical properties, Elaeagnaceae family, Elaeagnus umbellata Thunb. (autumn olive) was worth investigated. In this investigation, we revealed antioxidant, antiproliferative and enzyme inhibition activities of the water, methanol, ethanol, acetone, ethyl acetate and hexane extracts of E. umbellata as well as contents of their phenolic, flavonoid, anthocyanin, ascorbic acid, lycopene and b-carotene. The antioxidant activity was screened by phosphomolybdenum assay...
January 27, 2017: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/28135667/phytoextraction-of-toxic-trace-elements-by-sorghum-bicolor-inoculated-with-streptomyces-pactum-act12-in-contaminated-soils
#20
Amjad Ali, Di Guo, Amanullah Mahar, Ping Wang, Fang Ma, Feng Shen, Ronghua Li, Zengqiang Zhang
The increasing industrial, mining and agricultural activities have intensified the release of potential toxic trace elements (PTEs), which are of great concern to human health and environment. The alarming increase in PTEs concentration, stress the need for biotechnological remediation approaches. In order to assist phytoextraction of PTEs, different combinations of Streptomyces pactum (Act12) with biochar were applied to mining and industrial polluted soils of Shaanxi and Hunan Provinces of China, respectively...
January 27, 2017: Ecotoxicology and Environmental Safety
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