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https://www.readbyqxmd.com/read/28428780/genomic-and-transcriptomic-insights-into-calcium-carbonate-biomineralization-by-marine-actinobacterium-brevibacterium-linens-bs258
#1
Yuying Zhu, Ning Ma, Weihua Jin, Shimei Wu, Chaomin Sun
Calcium carbonate (CaCO3) biomineralization has been investigated due to its wide range of scientific and technological implications, however, the molecular mechanisms of this important geomicrobiological process are largely unknown. Here, a urease-positive marine actinobacterium Brevibacterium linens BS258 was demonstrated to effectively form CaCO3 precipitates. Surprisingly, this bacterium could also dissolve the formed CaCO3 with the increase of the Ca(2+) concentration. To disclose the mechanisms of biomineralization, the genome of B...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28425268/efficient-production-of-nanobodies-against-urease-activity-ofhelicobacter-pylori-in-pichia-pastoris
#2
Shahrbanoo Pourasadi, Seyed Latif Mousavi Gargari, Masoumeh Rajabibazl, Shahram Nazarian
BACKGROUND/AIM: Helicobacter pylori is a major health problem. One of the therapeutic approaches is administration of antibody against H. pylori. The methylotrophic Pichia pastoris is a suitable host for expression of recombinant antibody fragments. The aims of this study were the expression and the evaluation of camelid nanobody in the yeast Pichia pastoris. MATERIALS AND METHODS: The camelid-derived heavy-chain antibody (nanobody) against the UreC subunit of urease from H...
April 18, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28411636/armored-urease-enzyme-bioconjugated-poly-acrylamide-hydrogel-as-a-storage-and-sensing-platform
#3
Konda R Kunduru, S N Raju Kutcherlapati, Dhamodaran Arunbabu, Tushar Jana
Jack bean urease is an important enzyme not only because of its numerous uses in medical and other fields but also because of its historical significance-the first enzyme to be crystallized and also the first nickel metalloenzyme. This enzyme hydrolyzes urea into ammonia and carbon dioxide; however, the stability of this enzyme at ambient temperature is a bottleneck for its applicability. To improve urease stability, it was immobilized on different substrates, particularly on polymeric hydrogels. In this study, the enzyme was coupled covalently with poly(acrylamide) hydrogel with an yield of 18μmol/cm(3)...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28411515/response-of-soil-microbial-activities-and-microbial-community-structure-to-vanadium-stress
#4
REVIEW
Xi-Yuan Xiao, Ming-Wei Wang, Hui-Wen Zhu, Zhao-Hui Guo, Xiao-Qing Han, Peng Zeng
High levels of vanadium (V) have long-term, hazardous impacts on soil ecosystems and biological processes. In the present study, the effects of V on soil enzymatic activities, basal respiration (BR), microbial biomass carbon (MBC), and the microbial community structure were investigated through 12-week greenhouse incubation experiments. The results showed that V content affected soil dehydrogenase activity (DHA), BR, and MBC, while urease activity (UA) was less sensitive to V stress. The average median effective concentration (EC50) thresholds of V were predicted using a log-logistic dose-response model, and they were 362mgV/kg soil for BR and 417mgV/kg soil for DHA...
April 12, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28411320/addition-of-straw-from-hyperaccumulator-plants-to-cadmium-contaminated-soil-increases-cadmium-uptake-by-loquat-seedlings
#5
Lijin Lin, Ming'an Liao, Xiulan Lv, Dong Liang, Hui Xia, Jin Wang, Xun Wang
The straw from three different cadmium (Cd) hyperaccumulators (Galinsoga parviflora, Youngia erythrocarpa, and Solanum photeinocarpum) was added to Cd-contaminated soil, and its effects on plant growth and Cd accumulation in loquat seedlings were evaluated. Straw from each of G. parviflora, Y. erythrocarpa, and S. photeinocarpum was added to Cd-contaminated soil before planting seedlings of two varieties of loquat (Dawuxing and Chuanzao). Addition of straw from G. parviflora and S. photeinocarpum increased the root and shoot biomasses of both loquat varieties, compared with that in the control...
May 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28408993/real-world-helicobacter-pylori-diagnosis-in-patients-referred-for-esophagoduodenoscopy-the-gap-between-guidelines-and-clinical-practice
#6
Dor Shirin, Shay Matalon, Benjamin Avidan, Efrat Broide, Haim Shirin
BACKGROUND AND AIMS: Histopathology is the most accurate test to detect H. pylori when performed correctly with unknown validity in daily practice clinic settings. We aimed to determine the rate of potentially false-negative H. pylori results that might be due to continued use of proton pump inhibitors (PPIs) in routine endoscopy practice. We also aimed to establish whether gastroenterologists recommend routine cessation of PPIs before esophagogastroduodenoscopy (EGD) and whether they regularly document that biopsies for H...
December 2016: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/28407946/manuka-honey-leptospermum-scoparium-inhibits-jack-bean-urease-activity-due-to-methylglyoxal-and-dihydroxyacetone
#7
Jana Rückriemen, Oliver Klemm, Thomas Henle
Manuka honey (Leptospermum scoparium) exerts a strong antibacterial effect. Bacterial enzymes are an important target for antibacterial compounds. The enzyme urease produces ammonia and enables bacteria to adapt to an acidic environment. A new enzymatic assay, based on photometric detection of ammonia with ninhydrin, was developed to study urease activity. Methylglyoxal (MGO) and its precursor dihydroxyacetone (DHA), which are naturally present in manuka honey, were identified as jack bean urease inhibitors with IC50 values of 2...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28395509/postoperative-helicobacter-pylori-infection-as-a-prognostic-factor-for-gastric-cancer-patients-after-curative-resection
#8
Da Hyun Jung, Yong Chan Lee, Jie-Hyun Kim, Hyunsoo Chung, Jun Chul Park, Sung Kwan Shin, Sang Kil Lee, Hyoung-Il Kim, Woo Jin Hyung, Sung Hoon Noh
Background/Aims: Few studies have evaluated the effect of Helicobacter pylori infection on the prognosis of patients diagnosed with gastric cancer (GC) after curative surgery. We investigated the association between the H. pylori infection status and clinical outcome after surgery. Methods: We assessed the H. pylori status of 314 patients who underwent curative resection for GC. The H. pylori status was examined using a rapid urease test 2 months after resection...
April 11, 2017: Gut and Liver
https://www.readbyqxmd.com/read/28388008/an-expedient-synthesis-of-n-1-5-mercapto-4-substituted-benzylidene-amino-4h-1-2-4-triazol-3-yl-2-phenylethyl-benzamides-as-jack-bean-urease-inhibitors-and-free-radical-scavengers-kinetic-mechanism-and-molecular-docking-studies
#9
Aamer Saeed, Fayaz Ali Larik, Pervaiz Ali Channar, Haroon Mehfooz, Mohammad Haseeb Ashraf, Qamar Abbas, Mubashir Hassan, Sung-Yum Seo
In the present study some new azomethine-triazole hybrids 5a-5l derived from N-benzoyl-L-phenylalanine were synthesized and characterized. The synthesized compounds showed first-rate, urease inhibition and compounds 5c and 5e were found to be most effective inhibitors with 0.0137±0.00082 μM and 0.0183±0.00068 μM respectively (thiourea 15.151±1.27 μM). The kinetic mechanism of urease inhibition revealed the compounds 5c and 5e to be non-competitive inhibitors, whereas compounds 5d and 5j were found to be of mixed type inhibitors...
April 7, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28387235/streptomyces-pactum-assisted-phytoremediation-in-zn-pb-smelter-contaminated-soil-of-feng-county-and-its-impact-on-enzymatic-activities
#10
Amjad Ali, Di Guo, Amanullah Mahar, Fang Ma, Ronghua Li, Feng Shen, Ping Wang, Zengqiang Zhang
Anthropogenic activities, such as industrial expansion, smelting, mining and agricultural practices, have intensified the discharge of potentially toxic trace elements (PTEs) into the environment, threatening human health and other organisms. To assist phytoremediation by sorghum in soil contaminated by smelters/mines in Feng County (FC), a pot experiment was performed to examine the phytoremediation potential of Streptomyces pactum (Act12) + biochar. The results showed that root uptake of Zn and Cd was reduced by 45 and 22%, respectively, while the uptake of Pb and Cu increased by 17 and 47%, respectively...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28384543/novel-organophosphorus-scaffolds-of-urease-inhibitors-obtained-by-substitution-of-morita-baylis-hillman-adducts-with-phosphorus-nucleophiles
#11
Vassilis Ntatsopoulos, Stamatia Vassiliou, Katarzyna Macegoniuk, Łukasz Berlicki, Artur Mucha
The reactivity of Morita-Baylis-Hillman allyl acetates was employed to introduce phosphorus-containing functionalities to the side chain of the cinnamic acid conjugated system by nucleophilic displacement. The proximity of two acidic groups, the carboxylate and phosphonate/phosphinate groups, was necessary to form interactions in the active site of urease by recently described inhibitor frameworks. Several organophosphorus scaffolds were obtained and screened for inhibition of the bacterial urease, an enzyme that is essential for survival of urinary and gastrointestinal tract pathogens...
March 29, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28380613/influence-of-wet-heating-and-autoclaving-on-chemical-composition-and-standardized-ileal-crude-protein-and-amino-acid-digestibility-in-full-fat-soybeans-for-pigs
#12
C Kaewtapee, M Eklund, M Wiltafsky, H-P Piepho, R Mosenthin, P Rosenfelder
One batch each of eight full-fat soybeans (FFSB) was used to determine the effect of different heat treatments including wet heating (WH) and autoclaving (AC) on chemical composition and standardized ileal digestibility (SID) of CP and AA in growing pigs. The raw FFSB (K0) were either treated by WH at 80°C for 1 min (K1), at 100°C for 6 min (K2), or at 100°C for 16 min (K3). Thereafter, these batches were expanded at 125°C for 15 s. A further heat treatment included AC at 110°C for 15 (Z1), 30 (Z2), 45 (Z3), or 60 (Z4) min of FFSB that were subjected to the same WH treatment as K3...
February 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28379694/protein-tunnels-the-case-of-urease-accessory-proteins
#13
Francesco Musiani, Dario Gioia, Matteo Masetti, Federico Falchi, Andrea Cavalli, Maurizio Recanatini, Stefano Ciurli
Transition metals are both essential micronutrients and limited in environmental availability. The Ni(II)-dependent urease protein, the most efficient enzyme known to date, is a paradigm for studying the strategies that cells use to handle an essential, yet toxic, metal ion. Urease is a virulence factor of several human pathogens, in addition to decreasing the efficiency of soil organic nitrogen fertilization. Ni(II) insertion in the urease active site is performed through the action of three essential accessory proteins: UreD, UreF, and UreG...
April 5, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28355360/helicobacter-pylory-infection-in-patients-with-esophageal-squamous-cell-carcinoma
#14
Omer Bilgehan Poyrazoglu, Ahmet Cumhur Dulger, Bilge Sumbul Gultepe
OBJECTIVE: Esophageal squamous cell carcinoma is one of the most common esophageal diseases in the developing world, but the relationship between esophageal squamous cell carcinoma and Helicobacter pylori infection remains a neglected topic. The primary objective of this study was to determine the association between Helicobacter pylori infection and esophageal squamous cell carcinoma. A second purpose was to determine the incidence and factors associated with Helicobacter pylori infection following esophagectomy...
March 2017: Clinics
https://www.readbyqxmd.com/read/28351713/in%C3%A2-vitro-evaluation-of-cross-strain-inhibitory-effects-of-igy-polyclonal-antibody-against-h-%C3%A2-pylori
#15
Roya Solhi, Masoud Alebouyeh, Abolfazl Khafri, Morteza Rezaeifard, Mahdi Aminian
The present study aimed to evaluate in vitro cross-strain inhibitory effects of IgY polyclonal antibody on both growth and urease enzyme of four local strains of H. pylori. Leghorn chickens were immunized with whole cells of four different strains of H. pylori, separately. Rising of specific IgY was detected by ELISA. The IgY purified using polyethylene glycol method and the purity was evaluated by SDS-PAGE and Western blotting. Each strain was treated with its own-specific and also other strain-specific IgYs...
March 27, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28346872/5-bromo-2-aryl-benzimidazole-derivatives-as-non-cytotoxic-potential-dual-inhibitors-of-%C3%AE-glucosidase-and-urease-enzymes
#16
Tanzila Arshad, Khalid Mohammed Khan, Najma Rasool, Uzma Salar, Shafqat Hussain, Humna Asghar, Mohammed Ashraf, Abdul Wadood, Muhammad Riaz, Shahnaz Perveen, Muhammad Taha, Nor Hadiani Ismail
On the basis of previous report on promising α-glucosidase inhibitory activity of 5-bromo-2-aryl benzimidazole derivatives, these derivatives were further screened for urease inhibitory and cytotoxicity activity in order to get more potent and non-cytotoxic potential dual inhibitor for the patients suffering from diabetes as well as peptic ulcer. In this study, all compounds showed varying degree of potency in the range of (IC50=8.15±0.03-354.67±0.19μM) as compared to standard thiourea (IC50=21.25±0.15μM)...
March 20, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28345829/high-fluoroquinolone-resistant-strains-of-helicobacter-pylori-in-the-golden-triangle
#17
Ratha Korn Vilaichone, Thawee Ratanachu ek, Pornpen Gamnarai, Phawinee Subsomwong, Tomahisa Uchida, Yoshio Yamaoka, Varocha Mahachai
Background and aims: Helicobacter pylori (H. pylori) infections, associated with fatal GI diseases such as gastric cancer and MALT lymphoma, remain a major health problem in ASEAN countries. The Golden triangle has long been known as one of Asia’s main opium-producing areas. There have been no prior studies of H. pylori infection in this area. The major objectives of this project were therefore to establish prevalence, antibiotic resistance patterns and associated predictive in the Golden triangle. Methods: We recruited dyspeptic patients in Chiang khong and Chiang saen districts, Chiangrai province of Thailand...
February 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28345276/a-prospective-evaluation-of-levofloxacin-based-triple-therapy-for-refractory-helicobacter-pylori-infection-in-australia
#18
Ph Katelaris, Al Katelaris
BACKGROUND: First line Helicobacter pylori eradication failure is a common and challenging problem. AIMS: To assess the efficacy of salvage levofloxacin-based triple therapy in Australia. METHODS: Prospective patients referred after prior treatment failure(s) were prescribed esomeprazole 40 mg, amoxicillin 1 g and levofloxacin 500 mg each twice daily for ten days. All patients received detailed written and verbal adherence support. Outcome assessment was by (13) C-urea breath test and/or histology and urease test...
March 27, 2017: Internal Medicine Journal
https://www.readbyqxmd.com/read/28341531/urease-activity-as-an-index-for-assessing-the-maturity-of-cow-manure-and-wheat-residue-vermicomposts
#19
Saber Tayebi Sudkolai, Farshid Nourbakhsh
The establishment of a reliable index is an essential need to assess the degree of stability and maturity of solid wastes vermicomposts. The objective of this study was to investigate the effects of vermicomposting process on some chemical (pH, EC, OC, TN, lignin and C:N ratio) and biochemical properties of the cow manure (CM) and wheat residue (WR). Results demonstrated that during vermicomposting process of CM and WR urease activity was highly correlated with the time of vermicomposting (r=-0.97(∗∗) for CM and r=-0...
March 21, 2017: Waste Management
https://www.readbyqxmd.com/read/28337217/cost-effectiveness-analysis-of-helicobacter-pylori-diagnostic-methods-in-patients-with-atrophic-gastritis
#20
Fumio Omata, Takuro Shimbo, Sachiko Ohde, Gautam A Deshpande, Tsuguya Fukui
Background. There are several diagnostic methods for Helicobacter pylori (H. pylori) infection. A cost-effective analysis is needed to decide on the optimal diagnostic method. The aim of this study was to determine a cost-effective diagnostic method in patients with atrophic gastritis (AG). Methods. A decision-analysis model including seven diagnostic methods was constructed for patients with AG diagnosed by esophagogastroduodenoscopy. Expected values of cost and effectiveness were calculated for each test...
2017: Gastroenterology Research and Practice
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