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Mehdi Tourani, Maryam Habibzadeh, Ahmad Karkhah, Javad Shokri-Shirvani, Ladan Barari, Hamid Reza Nouri
Peptic ulcer is a lesion in the mucosa of the digestive tract affecting many people all around the world. Recent investigations have indicated that produced inflammatory cytokines such as TNF-α and IL-1β in response to gastric infection by Helicobacter pylori play an important role in the development of peptic ulcer. With regard to the significance of these cytokines in peptic ulcer development and the high prevalence of this disease in the developing countries, this study aimed to investigate the association of TNF-α and IL-1β with peptic ulcer in the presence of H...
February 15, 2018: Cytokine
Hua Fang, Lingxi Han, Houpu Zhang, Yanfei Deng, Qiqing Ge, Jiajia Mei, Zhengnan Long, Yunlong Yu
The dissipation of ciprofloxacin (CIP, 1.0 and 10.0mg/kg) and kresoxim-methyl (KM, 1.0 and 2.0mg/kg) in manure-amended soil, the variations in soil enzyme activities and microbial functional diversities, and CIP-induced bacterial community tolerances were studied using a chromatographic analysis, enzyme colorimetric and titration analyses, and the BIOLOG EcoPlate method. Three successive treatments of individual and combined samples of CIP and KM at low and high concentrations were performed at 60d intervals...
February 14, 2018: Science of the Total Environment
Petar Knezevic, Verica Aleksic Sabo, Natasa Simin, Marija Lesjak, Neda Mimica-Dukic
Helicobacter pylori is a major infective etiological agent of the upper gastrointestinal tract diseases. The bacterium exhibits resistance to various conventional antibiotics, being usually challenging for eradication. Since there is an urge to consider alternative therapeutic strategies, the aim of the study was to examine selected essential oils of plants belonging to families Cupressaceae (Juniperus communis) and Lamiaceae (Hyssopus officinalis, Salvia officinalis, Melissa officinalis, Lavandula angustifolia, Ocimum basilicum and Thymus serpyllum) against H...
February 8, 2018: Journal of Pharmaceutical and Biomedical Analysis
Sayaka Hirukawa, Hiroshi Sagara, Satoshi Kaneto, Tomoyo Kondo, Kotaro Kiga, Takahito Sanada, Hiroshi Kiyono, Hitomi Mimuro
Helicobacter pylori (H. pylori) is a Gram-negative microaerophilic bacterial pathogen which colonizes the stomach of more than half the human population, and is linked to chronic gastritis, peptic ulcers, and gastric cancer. Spiral-shaped H. pylori undergo morphologic conversion to the viable but not culturable (VBNC) coccoid form as they transit from the microaerobic stomach into the anaerobic intestinal tract. However, little is known about the morphological and pathogenic characteristics of H. pylori under prolonged anaerobic conditions...
February 15, 2018: Microbiology and Immunology
Mériem Merrouch, Martino Benvenuti, Marco Lorenzi, Christophe Léger, Vincent Fourmond, Sébastien Dementin
Nickel-containing enzymes are diverse in terms of function and active site structure. In many cases, the biosynthesis of the active site depends on accessory proteins which transport and insert the Ni ion. We review and discuss the literature related to the maturation of carbon monoxide dehydrogenases (CODH) which bear a nickel-containing active site consisting of a [Ni-4Fe-4S] center called the C-cluster. The maturation of this center has been much less studied than that of other nickel-containing enzymes such as urease and NiFe hydrogenase...
February 14, 2018: Journal of Biological Inorganic Chemistry: JBIC
Scarlet Milo, Florianne B Acosta, Hollie J Hathaway, Laura A Wallace, Naing T Thet, A Toby A Jenkins
Formation of crystalline biofilms following infection by Proteus mirabilis can lead to encrustation and blockage of long-term indwelling catheters, with serious clinical consequences. We describe a simple sensor, placed within the catheter drainage bag, to alert of impending blockage via a urinary color change. The pH-responsive sensor is a dual-layered polymeric "lozenge", able to release the self-quenching dye 5(6)-carboxyfluorescein in response to the alkaline urine generated by the expression of bacterial urease...
February 14, 2018: ACS Sensors
M L Cuellar-Nuñez, I Luzardo-Ocampo, R Campos-Vega, M A Gallegos-Corona, E González de Mejía, G Loarca-Piña
Moringa (Moringa oleifera) is a plant that has generated great interest in recent years because of its attributed medicinal properties. The aim of this study was to characterize the bioactive compounds of moringa leaves (MO) and evaluate their effect on a colorectal carcinogenesis model. Twenty-four male CD-1 mice were divided into 4 groups: Group 1 fed with basal diet (negative control/NC); Group 2 received AOM/DSS (positive control); Groups 3 and 4 were fed with basal diet supplemented with moringa leaves (2...
March 2018: Food Research International
Hui Zhu, Qing-Wei Zhou, Bai-Xing Yan, Yin-Xiu Liang, Xiang-Fei Yu, Yoram Gerchman, Xian-Wei Cheng
In this study, the influence of vegetation type and environmental temperature on performance of constructed wetlands (CWs) was investigated. Results of vegetation types indicated that the removal of most nutrients in polyculture was greater than those in monoculture and unplanted control. The greatest removal percentages of NH4+-N, total nitrogen (TN) and total phosphorus (TP) in polyculture were 98.7%, 98.5%, and 92.6%, respectively. In experiments of different temperatures, the removal percentages of NH4+-N, NO3--N, TN and TP in all CWs tended to decrease with the decline of temperature...
February 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Sekar Selvarajan, Ayyadurai Suganthi, Muthuramalingam Rajarajan
A novel zinc phthalocyanine/graphene oxide (ZnPh/GO) nanocomposite modified glassy carbon electrode (GCE) was prepared by using sonochemical approach and simple drop casting method. Urease (Urs) was used as the specific enzyme for urea detection and was physically immobilized onto the surface of ZnPh/GO nanocomposite. The fabricated ZnPh/GO/Urs matrix was successfully characterized by UV-vis-spectroscopy, FT-IR spectroscopy, scanning electron microscopy (SEM), raman spectrum, thermogravimetric analysis, cyclic voltammetric (CV) and amperometric techniques...
April 2018: Ultrasonics Sonochemistry
Tiehu He, Deyan Liu, Junji Yuan, Jiafa Luo, Stuart Lindsey, Nanthi Bolan, Weixin Ding
The effects of biochar combined with the urease inhibitor, hydroquinone, and nitrification inhibitor, dicyandiamide, on gaseous nitrogen (N2O, NO and NH3) emissions and wheat yield were examined in a wheat crop cultivated in a rice-wheat rotation system in the Taihu Lake region of China. Eight treatments comprised N fertilizer at a conventional application rate of 150kgNha-1 (CN); N fertilizer at an optimal application rate of 125kgNha-1 (ON); ON+wheat-derived biochar at rates of 7.5 (ONB1) and 15tha-1 (ONB2); ON+nitrification and urease inhibitors (ONI); ONI+wheat-derived biochar at rates of 7...
February 8, 2018: Science of the Total Environment
Fayez Raiesi, Mahshid Razmkhah, Shahram Kiani
The objective of this study was to determine responses of soil nitrogen (N) transformation, microbial biomass N, and urease activity to the combined effect of cadmium (Cd) toxicity (0 and 30 mg kg-1) and NaCl stress (0, 7.5 and 15 dS m-1) in a clay loam soil unamended (0%) or amended with alfalfa residues (1%, w/w). Cd, NaCl, and alfalfa residues were added to the soil, and the mixtures were incubated for 90 days under standard laboratory conditions (25 ± 1 °C and 70% of water holding capacity [WHC])...
February 7, 2018: Ecotoxicology and Environmental Safety
Chelsie E Armbruster, Harry L T Mobley, Melanie M Pearson
Proteus mirabilis , a Gram-negative rod-shaped bacterium most noted for its swarming motility and urease activity, frequently causes catheter-associated urinary tract infections (CAUTIs) that are often polymicrobial. These infections may be accompanied by urolithiasis, the development of bladder or kidney stones due to alkalinization of urine from urease-catalyzed urea hydrolysis. Adherence of the bacterium to epithelial and catheter surfaces is mediated by 17 different fimbriae, most notably MR/P fimbriae...
February 2018: EcoSal Plus
Ángel Benito, Fernando Calderón, Santiago Benito
The traditional way of producing wine is through the use of Saccharomyces cerevisiae in order to convert glucose and fructose into alcohol. In the case of red wines, after this alcoholic fermentation lactic bacteria Oenococus oeni is used to stabilize wine from a microbiological point of view by converting malic acid into lactic acid that it is not a microbiological substract. The yeast species Schizosaccharomyces pombe was traditionally considered spoilage yeast. Nevertheless, during the last decade it started to be used due to its unique malic acid deacidification ability to reduce the harsh acidity of wines from northern Europe, by converting malic acid to ethanol and CO2 without producing lactic acid as lactic bacteria does...
2018: Methods in Molecular Biology
Christopher D Burt, Miguel L Cabrera, Michael J Rothrock, D E Kissel
Broiler () litter is subject to ammonia (NH) volatilization losses. Previous work has shown that the addition of gypsum to broiler litter can increase nitrogen mineralization and decrease NH losses due to a decrease in pH, but the mechanisms responsible for these effects are not well understood. Therefore, three laboratory studies were conducted to evaluate the effect of gypsum addition to broiler litter on (i) urease activity at three water contents, (ii) calcium carbonate precipitation, and (iii) pH. The addition of gypsum to broiler litter increased ammonium concentrations ( < 0...
January 2018: Journal of Environmental Quality
Evelyne Mann, Stefanie U Wetzels, Martin Wagner, Qendrim Zebeli, Stephan Schmitz-Esser
Microbiota of the rumen wall constitute an important niche of rumen microbial ecology and their composition has been elucidated in different ruminants during the last years. However, the knowledge about the function of rumen wall microbes is still limited. Rumen wall biopsies were taken from three fistulated dairy cows under a standard forage-based diet and after 4 weeks of high concentrate feeding inducing a subacute rumen acidosis (SARA). Extracted RNA was used for metatranscriptome sequencing using Illumina HiSeq sequencing technology...
2018: Frontiers in Microbiology
Wenjuan Zhang, Li Ruirui, Xiaoyan Ai, Jiao Chen, Wennian Xu, Wei Li, Yingwei Ai
Large-scale railway construction has resulted in large areas of bare-cut-slope, and outside soil spray seeding (OSSS), a frequently used technique, has been adopted for slope restoration for many years. However, compared with natural slope soils, the quality of artificial soils on rock-cut slopes is low. Enzyme activity and microbial biomass are the main indices used for estimating soil quality; thus, our objective was to explore the influence of slope position, slope aspect, and season on two important factors that positively influence the plant growth capability in artificial soil...
January 31, 2018: Journal of Environmental Management
M Govarthanan, R Mythili, T Selvankumar, S Kamala-Kannan, H Kim
In the present study, Helianthus annuus grown in arsenic- (As) and lead- (Pb) contaminated soil were treated with plant-growth promoting fungi Trichoderma sp. MG isolated from decayed wood and assessed for their phytoremediation efficiency. The isolate MG exhibited a high tolerance to As (650mg/L) and Pb (500mg/L), and could remove > 70% of metals in aqueous solution with an initial concentration of 100mg/L each. In addition, the isolate MG was screened for plant-growth-promoting factors such as siderophores, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, indole acetic acid (IAA) synthesis, and phosphate solubilisation...
January 23, 2018: Ecotoxicology and Environmental Safety
Abdul Wahab, Syed Aleem Jan, Abdur Rauf, Zia Ur Rehman, Zahid Khan, Aftab Ahmed, Fatima Syed, Sher Zaman Safi, Hamayun Khan, Muhammad Imran
OBJECTIVE: Scandix pecten-veneris L. is a less studied wild edible herb and is considered an extinct plant species in many parts of the world. This study was designed to evaluate its phytochemical composition and biological potential of S. pecten-veneris L. METHODS: Phytochemicals including alkaloids, flavonoids, polyphenols, and tannins were determined in extracts of S. pecten-veneris. Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), while reducing power was tested by ferric reducing/antioxidant power (FRAP) assay...
2018: Journal of Zhejiang University. Science. B
Raul Torres Rodrigues, Paulo Victor Morais, Cristina S F Nordi, Michael J Schoning, José Roberto Siqueira Junior, Luciano Caseli
Algal polysaccharides (EPS) and carbon nanotubes (CNT) were adsorbed on dioctadecyldimethylammonium bromide (DODAB) Langmuir monolayers to serve as a matrix for the incorporation of urease. The physico-chemical properties of the supramolecular system as a monolayer at the air-water interface were investigated by surface pressure-area isotherms, surface potential-area isotherms, interfacial shear rheology, vibrational spectroscopy and Brewster angle microscopy. The floating monolayers were transferred to hydrophilic solid supports, quartz, mica or capacitive electrolyte-insulator-semiconductor (EIS) devices, through the Langmuir-Blodgett (LB) technique, forming mixed films, which were investigated by quartz crystal microbalance, fluorescence spectroscopy, and field-emission gun scanning electron microscopy...
February 3, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Manpreet Kaur, Harjodh Singh, Shivani Sharma, Sunita Mishra, Naga Radha Srinivas Tanuku, Anil Kumar Pinnaka
A novel Gram-negative, rod shaped, non-motile bacterium, designated strain YK2T, was isolated from yak milk from Leh, India. The strain was positive for oxidase- and catalase-activities and negative for starch hydrolysis, nitrate reduction, citrate utilization, urease, lysine decarboxylase and ornithine decarboxylase activities. The predominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C17 : 1ω9c and C16 : 1ω7c and/or C16 : 1ω6c and/or iso-C15 : 0 2-OH (summed feature 3)...
February 2018: International Journal of Systematic and Evolutionary Microbiology
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