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https://www.readbyqxmd.com/read/29053368/phytostabilization-potential-of-ornamental-plants-grown-in-soil-contaminated-with-cadmium
#1
Peng Zeng, Zhaohui Guo, Xia Cao, Xiyuan Xiao, Yanan Liu, Lei Shi
In a greenhouse experiment, five ornamental plants, Osmanthus fragrans (OF), Ligustrum vicaryi L. (LV), Cinnamomum camphora (CC), Loropetalum chinense var. rubrum (LC), and Euonymus japonicas cv. Aureo-mar (EJ), were studied for the ability to phytostabilization for Cd-contaminated soil. The results showed that these five ornamental plants can grow normally when the soil Cd content is less than 24.6 mg·kg(-1). Cd was mainly deposited in the roots of OF, LV, LC and EJ which have grown in Cd-contaminated soils, and the maximum Cd contents reached 15...
October 20, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/29052391/-research-on-bacteria-microecology-in-root-rot-rhizosphere-soil-of-coptis-chinensis-produced-in-shizhu-city
#2
Xu-Hong Song, Yu Wang, Long-Yun Li, Jun Tan
Illumina Hiseq 2500 high-throughput sequencing platform was used to study the bacteria richness and diversity, the soil enzyme activities, nutrients in unplanted soil, root-rot and healthy rhizophere soil of Coptis chinensis for deeply discussing the mechanism of the root-rot of C. chinensis. The high-throughput sequencing result showed that the artificial cultivation effected the bacteria community richness and diversity. The bacteria community richness in healthy and diseased rhizosphere soil showed significant lower than that of in unplanted soil (P<0...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29050151/-relationship-between-helicobacter-pylori-infection-and-mir-24-3p-expression-during-the-development-of-gastric-mucosa-lesions
#3
C Li, Y L Zhu, Q Li, D S Li, G B Yang
Objectives: To explore the expression of micro RNA-24-3p (miR-24-3p) in different gastric mucosa lesions, and analyze the potential correlation between Helicobacter pylori (H.pylori) infection and miR-24-3p expression in different gastric lesions. Methods: 158 gastric biopsy specimens were divided into four groups, including 35 chronic superficial gastritis (CSG) samples, 43 chronic atrophic gastritis (CAG) samples, 41 intestinal metaplasia (IM) samples and 39 dysplasia (Dys) samples. Those samples were collected from patients undergoing gastroscopy at the Department of Gastroenterology, Aerospace Center Hospital, from September 2005 to June 2012...
September 26, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29040823/urea-increased-nickel-and-copper-accumulation-in-the-leaves-of-egeria-densa-planch-casp-and-ceratophyllum-demersum-l-during-short-term-exposure
#4
Maria Maleva, Galina Borisova, Nadezhda Chukina, Adarsh Kumar
In the present study, two fresh water plant species Egeria densa (Planch.) Casp. and Ceratophyllum demersum L. were subjected to separate and combined action of urea (2mМ) and metals (Ni and Cu, 10μM) to investigate the phytoremediation potential of these two submerged macrophytes during short-term experiments (48h). Both submerged macrophytes demonstrated high accumulative potential for Ni and Cu (average bioconcentration factors were 2505 for Ni and 3778 for Cu). The urea (2 mM) was not significantly toxic for studied plant species...
October 14, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29039202/nanoelectronic-platform-for-ultrasensitive-detection-of-protein-biomarkers-in-serum-using-dna-amplification
#5
Luye Mu, Ilia A Droujinine, Jieun Lee, Mathias Wipf, Paschall Davis, Chris Adams, Jennifer Hannant, Mark A Reed
Silicon nanowire field effect transistors (NWFETs) are low noise, low power, ultrasensitive biosensors that are highly amenable to integration. However, using NWFETs to achieve direct protein detection in physiological buffers such as blood serum remains difficult due to Debye screening, nonspecific binding, and stringent functionalization requirements. In this work, we performed an indirect sandwich immunoassay in serum combined with exponential DNA amplification and pH measurement by ultrasensitive NWFET sensors...
October 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29037122/obtaining-edaphic-biostimulants-biofertilizers-from-sewage-sludge-using-fermentative-processes-short-time-effects-on-soil-biochemical-properties
#6
Bruno Rodríguez-Morgado, Pablo Caballero, Patricia Paneque, Isidoro Gómez, Juan Parrado, Manuel Tejada
In this manuscript we study the manufacture and effect on soils of different edaphic biostimulants/biofertilizers (BS) obtained from sewage sludge using Bacillus licheniformis as biological tool. These BS consist of different combinations of organic matter, bacteria and enzymes that were subjected to several treatments. These BS were applied in soil in order to observe their influence on the biochemical properties (enzymatic activities and ergosterol content). Dehydrogenase, urease, β-glucosidase, phosphatase activities and ergosterol content were measured at different incubation days...
October 16, 2017: Environmental Technology
https://www.readbyqxmd.com/read/29034633/-comparison-of-effects-of-staphylococcal-nuclease-a-fused-with-different-exogenous-dna-fragments
#7
Lixia Fu, Dejun Ji, Xubin Lu, Xian'gan Han, Wenzhi Wei
Staphylococcal nuclease A (SNA) may be used to produce bacterial ghosts for further inactivation of host bacteria and elimination of residual genetic materials. It is still controversial if SNA without signal peptide can be secreted to extracellular matrix and if fusion with other peptide is required for its function in the cytoplasm of host bacteria. To clarify this dispute, a series of temperature-inducible plasmids carrying SNA alone or SNA fused with partial sequences of λ phage cro gene (cSNA) or Mycobacterium tuberculosis urease gene (uSNA) were constructed and evaluated in Escherichia coli...
December 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29032032/enzyme-inhibitory-activities-an-insight-into-the-structure-activity-relationship-of-biscoumarin-derivatives
#8
Muhammad Faisal, Aamer Saeed, Danish Shahzad, Tanzeela Abdul Fattah, Bhajan Lal, Pervaiz Ali Channar, Jamaluddin Mahar, Shomaila Saeed, Parvez Ali Mahesar, Fayaz Ali Larik
Biscoumarin derivatives, a dimeric form of coumarin, are well known derivatives of coumarin, occurred in the bioactive metabolites of marine and terrestrial organisms. On account of pharmacological and biological applications, biscoumarins have long been the subject of innumerable enzyme inhibition studies. In this review the pros and cons of enzyme inhibition studies of biscoumarins as urease inhibitors, aromatase inhibitors, NPPs, α-glucosidase inhibitors, α-amylase inhibitors, HIV-1 integrase inhibition, steroid sulfatase inhibitors and c-Met inhibitors are discussed in a systematic way...
October 6, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29031169/3-4-dimethoxybenzohydrazide-derivatives-as-antiulcer-molecular-modeling-and-density-functional-studies
#9
Muhammad Taha, Nor Hadiani Ismail, Hamizah Mohd Zaki, Abdul Wadood, El Hassane Anouar, Syahrul Imran, Bohari M Yamin, Fazal Rahim, Muhammad Ali, Khalid Mohammed Khan
3,4-Dimethoxybenzohydrazide derivatives (1-25) have been synthesized and evaluated for their urease inhibitory potential. Among the series, compounds 2, 3, 4 and 5 with IC50 values 12.61 ± 0.07, 18.24 ± 0.14, 19.22 ± 0.21, and 8.40 ± 0.05 µM, respectively, showed excellent urease inhibitory potentials when compared with standard thiourea (IC50 value 21.40 ± 0.21 µM). Compounds 1, 6, 8, 18, 19 and 20 also showed good to moderate inhibition, while the remaining compounds were found to be completely inactive...
October 7, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29023067/studies-on-staphylococcus-aureus-isolated-from-pimples
#10
Ajayi Adesola Adetutu, Bibi Oritsewehinmi, Oniha Margaret Ikhiwili, Atolagbe Oluwabunmi Moradeke, Anosike Selina Odochi, Onibokun Elizabeth Adeola
BACKGROUND AND OBJECTIVE: Pimples (acne) are small skin lesions or inflammations of the skin. The most common factor causing acne is the hormonal changes that occur during adolescent and teenage years. Antibiotics are becoming less effective in the treatment of pimples due to increasing concerns of antibiotic resistance. This study was therefore carried out to characterize the isolates from the pimples of Covenant University Students and to determine their antibiotics sensitivity pattern...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29021786/a-new-role-for-helicobacter-pylori-urease-contributions-to-angiogenesis
#11
Deiber Olivera-Severo, Augusto F Uberti, Miguel S Marques, Marta T Pinto, Maria Gomez-Lazaro, Céu Figueiredo, Marina Leite, Célia R Carlini
Helicobacter pylori is a pathogen involved in gastric diseases such as ulcers and carcinomas. H. pylori's urease is an important virulence factor produced in large amounts by this bacterium. In previous studies, we have shown that this protein is able to activate several cell types like neutrophils, monocytes, platelets, endothelial cells, and gastric epithelial cells. Angiogenesis is a physiological process implicated in growth, invasion and metastization of tumors. Here, we have analyzed the angiogenic potential of H...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29019930/biological-evaluation-and-molecular-docking-of-protocatechuic-acid-from-hibiscus-sabdariffa-l-as-a-potent-urease-inhibitor-by-an-esi-ms-based-method
#12
Sherif T S Hassan, Emil Švajdlenka
Studies on enzyme inhibition remain a crucial area in drug discovery since these studies have led to the discoveries of new lead compounds useful in the treatment of several diseases. In this study, protocatechuic acid (PCA), an active compound from Hibiscus sabdariffa L. has been evaluated for its inhibitory properties against jack bean urease (JBU) as well as its possible toxic effect on human gastric epithelial cells (GES-1). Anti-urease activity was evaluated by an Electrospray Ionization-Mass Spectrometry (ESI-MS) based method, while cytotoxicity was assayed by the MTT method...
October 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29018994/proper-land-use-for-heavy-metal-polluted-soil-based-on-enzyme-activity-analysis-around-a-pb-zn-mine-in-feng-county-china
#13
Linchuan Fang, Yuqing Liu, Haixia Tian, Hansong Chen, Yunqiang Wang, Min Huang
Enzymes in the soil are useful for assessing heavy metal soil pollution. We analyzed the activity of a number of enzymes, including urease, protease, catalase, and alkaline phosphatase, in three types of land (farmland, woodland, and grassland) to evaluate soil pollution by heavy metals (Pb, Zn, and Cd). Our results showed that the tested soil was polluted by a combination of Pb, Zn, and Cd, but the primary pollutant was Cd. An ecological dose analysis demonstrated that urease was the most sensitive enzyme to Pb and Cd in the farmland, and catalase and phosphatase were the most sensitive enzymes to Pb, Zn, and Cd in the woodland and grassland...
October 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28991976/combining-urease-and-nitrification-inhibitors-with-incorporation-reduces-ammonia-and-nitrous-oxide-emissions-and-increases-corn-yields
#14
Craig F Drury, Xueming Yang, W Dan Reynolds, Wayne Calder, Tom O Oloya, Alex L Woodley
Less than 50% of applied nitrogen (N) fertilizer is typically recovered by corn ( L.) due to climatic constraints, soil degradation, overapplication, and losses to air and water. Two application methods, two N sources, and two inhibitors were evaluated to reduce N losses and enhance crop uptake. The treatments included broadcast urea (BrUrea), BrUrea with a urease inhibitor (BrUrea+UI), BrUrea with a urease and a nitrification inhibitor (BrUrea+UI+NI), injection of urea ammonium nitrate (InjUAN), and injected with one or both inhibitors (InjUAN+UI, InjUAN+UI+NI), and a control...
September 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28991971/effects-of-positively-charged-dicyandiamide-and-nitrogen-fertilizer-sources-on-nitrous-oxide-emissions-in-irrigated-corn
#15
Hannah Waterhouse, Jordon Wade, William R Horwath, Martin Burger
Synthetic nitrogen (N) fertilizer formulations vary in their effects as substrates on nitrous oxide (NO) emissions. Mitigation of NO emissions can potentially be achieved through appropriate choice of N fertilizer sources combined with stabilizers. The effects of three N fertilizers and urease and nitrification inhibitors on NO emissions, crop N uptake, and yields were determined in a furrow-irrigated corn ( L.) system in Reiff loam soil in the Sacramento Valley of California for one growing season. Aqua ammonia (Aq...
September 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28978104/the-influence-of-adjuvant-on-ureb-protection-against-helicobacter-pylori-through-the-diversity-of-cd4-t-cell-epitope-repertoire
#16
Bin Li, Hanmei Yuan, Li Chen, Heqiang Sun, Jian Hu, Shanshan Wei, Zhuo Zhao, Quanming Zou, Chao Wu
Adjuvants are widely used to enhance the effects of vaccines against pathogen infections. Interestingly, different adjuvants and vaccination routes usually induce dissimilar immune responses, and can even have completely opposite effects. The mechanism remains unclear. In this study, urease B subunit (UreB), an antigen of Helicobacter pylori (H. pylori) that can induce protective immune responses, was used as a model to vaccinate mice. We investigated the effects of different adjuvants and routes on consequent T cell epitope-specific targeting and protection against H...
September 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28977793/antibiotic-resistance-trends-in-methicillin-resistant-staphylococcus-aureus-isolated-in-kuwait-hospitals-2011-2015
#17
Edet Udo, Samar Boswihi
<br>The aim of this study was to determine antibiotic resistance trends and carriage of staphylococcal cassette chromosome mec (SCCmec) genetic elements in methicillin-resistant Staphylococcus aureus (MRSA) isolated in Kuwait hospitals to ascertain whether they were healthcare-associated (HA-MRSA) or community-associated (CA-MRSA). <br> Materials (Subjects) and Methods<br>In total 6,922 MRSA isolates obtained from different clinical samples were tested for resistance to antibiotics, urease production and carriage of SCCmec elements...
October 4, 2017: Medical Principles and Practice: International Journal of the Kuwait University, Health Science Centre
https://www.readbyqxmd.com/read/28974973/facile-one-pot-four-component-synthesis-of-3-4-dihydro-2-pyridone-derivatives-novel-urease-inhibitor-scaffold
#18
Arash Modarres Hakimi, Negar Lashgari, Shabnam Mahernia, Ghodsi Mohammadi Ziarani, Massoud Amanlou
In the current study, a series of 3,4-dihydro-2-pyridone derivatives were synthesized in a one-pot fourcomponent reaction of Meldrum's acid, benzaldehyde derivatives, methyl acetoacetate, and ammonium acetate. SiO2-Pr-SO3H was used as an efficient catalyst for the synthesis of the target compounds under solvent-free conditions. The most probable mechanism for this reaction has been discussed. The advantages of this methodology are high product yields, being environmentally benign, short reaction times, and easy handling...
October 2017: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28974949/the-recombinant-bacille-calmette-gu%C3%A3-rin-vaccine-vpm1002-ready-for-clinical-efficacy-testing
#19
REVIEW
Natalie E Nieuwenhuizen, Prasad S Kulkarni, Umesh Shaligram, Mark F Cotton, Cyrill A Rentsch, Bernd Eisele, Leander Grode, Stefan H E Kaufmann
The only licensed vaccine against tuberculosis (TB), bacille Calmette-Guérin (BCG), protects against severe extrapulmonary forms of TB but is virtually ineffective against the most prevalent form of the disease, pulmonary TB. BCG was genetically modified at the Max Planck Institute for Infection Biology to improve its immunogenicity by replacing the urease C encoding gene with the listeriolysin encoding gene from Listeria monocytogenes. Listeriolysin perturbates the phagosomal membrane at acidic pH. Urease C is involved in neutralization of the phagosome harboring BCG...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28973790/enhanced-efficiency-fertilizers-are-not-a-panacea-for-resolving-the-nitrogen-problem
#20
Tingyu Li, Weifeng Zhang, Jiao Yin, David Chadwick, David Norse, Yuelai Lu, Xuejun Liu, Xinping Chen, Fusuo Zhang, David Powlson, Zhengxia Dou
Improving nitrogen (N) management for greater agricultural output while minimizing unintended environmental consequences is critical in the endeavor of feeding the growing population sustainably amid climate change. Enhanced-efficiency fertilizers (EEFs) have been developed to better synchronize fertilizer N release with crop uptake, offering the potential for enhanced N use efficiency (NUE) and reduced losses. Can EEFs play a significant role in helping address the N management challenge? Here we present a comprehensive analysis of worldwide studies published in 1980-2016 evaluating four major types of EEFs (polymer-coated fertilizers PCF, nitrification inhibitors NI, urease inhibitors UI, and double inhibitors DI, i...
October 3, 2017: Global Change Biology
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