keyword
MENU ▼
Read by QxMD icon Read
search

urease

keyword
https://www.readbyqxmd.com/read/28633450/comparative-genomics-of-all-three-campylobacter-sputorum-biovars-and-a-novel-cattle-associated-c-sputorum-clade
#1
William G Miller, Emma Yee, Mary H Chapman, James L Bono
Campylobacter sputorum is a non-thermotolerant campylobacter that is primarily isolated from food animals such as cattle and sheep. C. sputorum is also infrequently associated with human illness. Based on catalase and urease activity, three biovars are currently recognized within C. sputorum: bv. sputorum (catalase negative, urease negative), bv. fecalis (catalase positive, urease negative), and bv. paraureolyticus (catalase negative, urease positive). A multi-locus sequence typing (MLST) method was recently constructed for C...
June 19, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28623137/azomethines-isoxazole-n-substituted-pyrazoles-and-pyrimidine-containing-curcumin-derivatives-urease-inhibition-and-molecular-modeling-studies
#2
Mahmood Ahmed, Muhammad Abdul Qadir, Abdul Hameed, Muhammad Nadeem Arshad, Abdullah M Asiri, Muhammad Muddassar
Curcumin has shown large number of pharmacological properties against different phenotypes of various disease models. Different synthetic routes have been employed to develop its various derivatives for diverse biological functions. In this study, curcumin derived azomethine, isoxazole, pyrimidines and N-substituted pyrazoles were synthesized to investigate their urease enzyme inhibition. The structures of newly synthesized compounds were described by IR, MS, (1)H NMR and (13)C NMR spectral data. Urease enzyme inhibition was evaluated through in vitro assays in which compound 8b was found to be the most potent (IC50 = 2...
June 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28618277/fate-and-adverse-effects-of-hexabromocyclododecane-diastereoisomers-hbcdds-in-a-soil-ryegrass-pot-system
#3
Hongkai Zhu, Hongwen Sun, Yiming Yao, Fei Wang, Yanwei Zhang, Xiaowei Liu
This study explored the fate and adverse effects of 3 main hexabromocyclododecane diastereoisomers (α-, β-, and γ-HBCDDs) in a soil pot system planted with ryegrass (Lolium perenne L.) using a short-term (8 weeks) experiment. At the end of the experiment, soil urease activity in planted spiked soil increased and catalase activity decreased; while there was no obvious change in sucrase and peroxidase activities. HBCDDs mainly accumulated in the root of ryegrass, with root concentration factors (RCF) in the range of 1...
May 31, 2017: Chemosphere
https://www.readbyqxmd.com/read/28616083/outer-membrane-phospholipase-a-s-roles-in-helicobacter-pylori-acid-adaptation
#4
Hilde S Vollan, Tone Tannæs, Dominique A Caugant, Gert Vriend, Geir Bukholm
BACKGROUND: The pH of the human gastric mucosa varies around 2.5 so that only bacteria with strong acidic stress tolerance can colonize it. The ulcer causing Helicobacter pylori thrives in the gastric mucosa. We analyse the roles of the key outer membrane protein OMPLA in its roles in acid tolerance. RESULTS: The homology model of Helicobacter pylori outer membrane phospholipase A (OMPLA) reveals a twelve stranded β-barrel with a pore that allows molecules to pass with a diameter up to 4 Å...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28615866/virulence-assay-and-role-of-bacillus-thuringiensis-ts110-as-biocontrol-agent-against-the-larval-stages-of-rice-leaffolder-cnaphalocrocis-medinalis
#5
Tuhin Subhra Ghosh, Soumendranath Chatterjee, Syed Afrin Azmi, Abhijit Mazumdar, Tushar Kanti Dangar
The bacterial isolate Bacillus thuringiensis TS110 was isolated from the rice field soil of Burdwan district, West Bengal, India. Bioassay test of the bacteria TS110 against 3rd, 4th and 5th instar larvae of Cnaphalocrocis medinalis was carried out. Cut leaf assay, potted plant assay and field assay were done. During filed assay, it has been observed that the LC50 (×10(7)) values of TS110 against 3rd, 4th and 5th instar larvae of C. medinalis were 3.77, 5.29, 4.83 and 4.93, 4.42, 4.72 in dry and wet season, respectively...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28605646/imidacloprid-application-changes-microbial-dynamics-and-enzymes-in-rice-soil
#6
Bibhab Mahapatra, Totan Adak, Naveen K B Patil, Guru P Pandi G, G Basana Gowda, N N Jambhulkar, Manoj Kumar Yadav, P Panneerselvam, Upendra Kumar, Sushmita Munda, Mayabini Jena
Extensive use of imidacloprid in rice ecosystem may alter dynamics of microorganisms and can change soil biochemical properties. The objective of this study was to assess the effect of imidacloprid on growth and activities of microbes in tropical rice soil ecosystem. Four treatments, namely, recommended dose (at 25g a.i. ha(-1), RD), double the recommended dose (at 50g a.i. ha(-1), 2RD), five times the recommended dose (at 125g a.i. ha(-1), 5RD) & ten times the recommended dose (at 250g a.i. ha(-1), 10RD) along with control were imposed under controlled condition...
June 9, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28602911/jaburetox-induced-toxic-effects-on-the-hemocytes-of-rhodnius-prolixus-hemiptera-reduviidae
#7
Natalia R Moyetta, Valquiria Broll, Ana Paula A Perin, Augusto F Uberti, Matheus V Coste Grahl, Fernanda Staniscuaski, Celia R Carlini, Leonardo L Fruttero
Jaburetox is a recombinant peptide derived from a Canavalia ensiformis urease that presents toxic effects upon several species of insects, phytopathogenic fungi and yeasts of medical importance. So far, no toxicity of Jaburetox to mammals has been shown. Previous reports have identified biochemical targets of this toxic peptide in insect models, although its mechanism of action is not completely understood. In this work, we aimed to characterize the effects of Jaburetox in hemolymphatic insect cells. For this purpose, the model insect and Chagas' disease vector Rhodnius prolixus was used...
June 8, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28601610/therapeutic-success-with-bismuth-containing-sequential-and-quadruple-regimens-in-helicobacter-pylori-eradication
#8
Oguzhan Ozturk, Levent Doganay, Yasar Colak, Feruze Yilmaz Enc, Celal Ulasoglu, Kamil Ozdil, Ilyas Tuncer
BACKGROUND AND STUDY AIMS: The success rate of Helicobacter pylori (H. pylori) eradication with the classical triple therapy is gradually declining. In this study, we aimed to compare and assess the efficacies of six different eradication regimens including sequential protocols. PATIENTS AND METHODS: Endoscopically confirmed nonulcer dyspepsia patients were enrolled. H. pylori presence was determined either histologically or by a rapid urease test. Treatment-naive patients were randomly assigned to an either one of three 10-day (OAC, OTMB, and OACB) or one of three sequential protocols (OA+OCM, OA+OCMB, and OA+OMDB) (O=omeprazole, A=amoxicillin, C=clarithromycin, T=tetracycline, M=metronidazole, B=bismuth, D=doxycycline)...
June 7, 2017: Arab Journal of Gastroenterology: the Official Publication of the Pan-Arab Association of Gastroenterology
https://www.readbyqxmd.com/read/28601120/measurement-of-breath-ammonia-for-detection-of-patients-with-chronic-kidney-disease
#9
Sebastjan Bevc, Eva Mohorko, Mitja Kolar, Polona Brglez, Andrej Holobar, Daniela Kniepeiss, Matej Podbregar, Nejc Piko, Nina Hojs, Maša Knehtl, Robert Ekart, Radovan Hojs
BACKGROUND: In a healthy individual, ammonia is converted to urea in the liver. Urea is then transported through the bloodstream and then excreted into the urine by the kidneys. In patients with chronic kidney disease (CKD), the accumulated urea is degraded by salivary urease into ammonia, which is then excreted by breathing. Breath ammonia can therefore be used for detecting the increased nitrogen-bearing wastes. In our pilot study, an electrochemical sensor was used to measure and analyze breath ammonia in healthy volunteers and patients with CKD...
June 9, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28596600/detection-of-dna-amplicons-of-polymerase-chain-reaction-using-litmus-test
#10
Dingran Chang, Kha Tram, Ben Li, Qian Feng, Zhifa Shen, Christine H Lee, Bruno J Salena, Yingfu Li
We report on a new colorimetric DNA detection method that takes advantage of the power of polymerase chain reaction (PCR) and the simplicity of the classic litmus test. The strategy makes use of a modified set of primers for PCR to facilitate ensuing manipulations of resultant DNA amplicons: their tagging with urease and immobilization onto magnetic beads. The amplicon/urease-laden beads are then used to hydrolyze urea, resulting in the increase of pH that can be conveniently reported by a pH-sensitive dye...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28593545/improved-strength-and-durability-of-concrete-through-metabolic-activity-of-ureolytic-bacteria
#11
Maria Jose Castro Alonso, Carlos Eloir Lopez Ortiz, Sixto Omar Garcia Perez, Rajeswari Narayanasamy, Gerardo Del Jesús Fajardo San Miguel, Héctor Herrera Hernández, Nagamani Balagurusamy
In recent years, biomineralization process is being employed in development of bioconcrete, which is emerging as a sustainable method to enhance the durability of concrete by way of increasing compressive strength and reducing the chloride permeability. In this study, different bacterial strains isolated from the soils of the Laguna Region of Mexico were selected for further study. ACRN5 strain demonstrated higher urease activity than other strains, and the optimum substrate concentration, pH, and temperature were 120 mM, pH 8, and 25 °C, respectively...
June 7, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28592011/-gas-chromatography-mass-spectrometry-based-urinary-metabolomics-in-very-low-birth-weight-premature-infants
#12
S T Li, X L Huang, S G Wu, Y M Ma, C C Shi, X Xiao, H Hao
Objective: To investigate the urinary metabolic spectrum and pathways in very low birth weight (VLBW) premature infants. Method: A prospective case-control study was conducted to collect and compare the data of VLBW premature infants and full term infants from the Sixth Affiliated Hospital of Sun Yet-Sen University in 2014. Within 24 hours after birth, urine specimens in each group were collected. Metabolites of urine samples including amino acid, fatty acid and organic acid were detected using the urease pre-processing and gas chromatography mass spectrometry (GC-MS) technology...
June 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/28585174/a-patent-landscape-on-application-of-microorganisms-in-construction-industry
#13
REVIEW
Dipti Dapurkar, Manasi Telang
Construction biotechnology includes research and development of construction materials and processes that make use of various microbes. The present technology landscape gives a perspective on how microbes have been used in construction industry as cement and concrete additives by analyzing patents filed in this technology arena. All patents related to the technology of interest published globally to date have been reviewed. The earliest patent filing in this technology domain was recorded in the year 1958 and the patenting activity reached its peak around mid to late 1990s...
July 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28581361/the-study-of-h-pylori-putative-candidate-factors-for-single-and-multi-component-vaccine-development
#14
Nasrin Mirzaei, Farkhondeh Poursina, Sharareh Moghim, Niloufar Rashidi, Hajieh Ghasemian Safaei
Helicobacter pylori has grown to colonize inside the stomach of nearly half of the world's population, turning into the most prevalent infections in the universe. Medical care failures noticeably confirm the need for a vaccine to hinder or deal with H. pylori. This review is planned to discuss the most known factors as a vaccine candidate, including single (AhpC, BG, CagA, KatA, Fla, Hsp, HWC, Lpp, LPS, NAP, OMP, OMV, SOD, Tpx, Urease, VacA) and multi-component vaccines. Many promising results in the field of single and multivalent vaccine can be seen, but there is no satisfactory outcome and neither a prophylactic nor a therapeutic vaccine to treat or eradicate the infection in human has been acquired...
September 2017: Critical Reviews in Microbiology
https://www.readbyqxmd.com/read/28581271/helicobacter-pylori-antigens-inducing-early-immune-response-in-infants
#15
Ji Hyun Seo, Jong Hyuk Youn, Eun A Kim, Jin Su Jun, Ji Sook Park, Jung Sook Yeom, Jae Young Lim, Hyang Ok Woo, Hee Shang Youn, Gyung Hyuck Ko, Jin Sik Park, Seung Chul Baik, Woo Kon Lee, Myung Je Cho, Kwang Ho Rhee
To identify the Helicobacter pylori antigens operating during early infection in sera from infected infants using proteomics and immunoblot analysis. Two-dimensional (2D) large and small gel electrophoresis was performed using H. pylori strain 51. We performed 2D immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) antibody immunoblotting using small gels on sera collected at the Gyeongsang National University Hospital from 4-11-month-old infants confirmed with H. pylori infection by pre-embedding immunoelectron microscopy...
July 2017: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/28578402/using-bamboo-biochar-with-compost-for-the-stabilization-and-phytotoxicity-reduction-of-heavy-metals-in-mine-contaminated-soils-of-china
#16
Amjad Ali, Di Guo, Yue Zhang, Xining Sun, Shuncheng Jiang, Zhanyu Guo, Hui Huang, Wen Liang, Ronghua Li, Zengqiang Zhang
Anthropogenic activities have transformed the global geochemical cycling of heavy metals (HMs). Many physical, chemical and biological methods are used to reduce the toxicity of HMs to humans, plants and environment. This study aimed to investigate the immobilization and phytotoxicity reduction of HMs after application of bamboo biochar (BB) in mine-polluted soil in Feng county (FC) and Tongguan (TG). The results showed that BB application to contaminated soil immobilized HMs (Zn, Pb, Cd and Cu). The soil pH and EC increased and the bioavailability of HMs decreased in FC and TG, whereas Pb and Cu increased in TG soil...
June 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28578263/inorganic-phosphorus-fertilizer-ameliorates-maize-growth-by-reducing-metal-uptake-improving-soil-enzyme-activity-and-microbial-community-structure
#17
Wencheng Wu, Jiahui Wu, Xiaowen Liu, Xianbin Chen, Yingxin Wu, Shixiao Yu
Recently, several studies have showed that both organic and inorganic fertilizers are effective in immobilizing heavy metals at low cost, in comparison to other remediation strategies for heavy metal-contaminated farmlands. A pot trial was conducted in this study to examine the effects of inorganic P fertilizer and organic fertilizer, in single application or in combination, on growth of maize, heavy metal availabilities, enzyme activities, and microbial community structure in metal-contaminated soils from an electronic waste recycling region...
June 1, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28575047/utility-of-normalized-genome-quantification-of-helicobacter-pylori-in-gastric-mucosa-using-an-in-house-real-time-polymerase-chain-reaction
#18
Ana Morilla, Santiago Melón, Marta E Álvarez-Argüelles, Edisa Armesto, Henar Villar, María de Oña
Traditional diagnostic assays for Helicobacter pylori detection have their limitations. Molecular methods can improve both diagnosis and understanding of gastric diseases. Here we describe an in-house quantitative real-time polymerase chain reaction (q-rt-PCR) for the detection of H. pylori in gastric biopsies which has been developed and has a detection limit of 10 copies, the specificity of which was tested against other gastric colonizer bacteria. In this study, 199 gastric biopsies from adults with different clinical gastric symptoms were examined...
2017: PloS One
https://www.readbyqxmd.com/read/28574649/hybrid-pharmacophoric-approach-in-the-design-and-synthesis-of-coumarin-linked-pyrazolinyl-as-urease-inhibitors-kinetic-mechanism-and-molecular-docking
#19
Aamer Saeed, Parvez Ali Mahesar, Pervaiz Ali Channar, Fayaz Ali Larik, Qamar Abbas, Mubashir Hassan, Hussain Raza, Sung-Yum Seo
The current research article reports the synthesis of coumarinyl pyrazolinyl thioamide derivatives and their biological activity as inhibitors of jack bean urease. The coumarinyl pyrazolinyl thioamides were synthesized by reacting thiosemicarbazide with newly synthesized chalcones to afford the products in good yields and the synthesized compounds were purified by recrystallization. Coumarinyl pyrazolinyl thioamide derivatives 5a-5q showed significant activity against Urease enzyme and also exhibited good antioxidant potential...
June 2, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28573847/nickel-sequestration-by-the-host-defense-protein-human-calprotectin
#20
Toshiki G Nakashige, Emily M Zygiel, Catherine L Drennan, Elizabeth M Nolan
The human innate immune protein calprotectin (CP, S100A8/S100A9 oligomer, calgranulin A/calgranulin B oligomer, MRP-8/MRP-14 oligomer) chelates a number of first-row transition metals, including Mn(II), Fe(II), and Zn(II), and can withhold these essential nutrients from microbes. Here we elucidate the Ni(II) coordination chemistry of human CP. We present a 2.6-Å crystal structure of Ni(II)- and Ca(II)-bound CP, which reveals that CP binds Ni(II) ions at both its transition-metal-binding sites: the His3Asp motif (site 1) and the His6 motif (site 2)...
June 22, 2017: Journal of the American Chemical Society
keyword
keyword
54603
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"