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https://www.readbyqxmd.com/read/28546954/pleiotropic-cytotoxicity-of-vaca-toxin-in-host-cells-and-its-impact-on-immunotherapy
#1
REVIEW
Farnaz Fahimi, Mohammad Reza Tohidkia, Mehdi Fouladi, Reza Aghabeygi, Naser Samadi, Yadollah Omidi
Introduction: In the recent decades, a number of studies have highlighted the importance of Helicobacter pylori in the initiation and development of peptic ulcer and gastric cancer. Some potential virulence factors (e.g., urease, CagA, VacA, BabA) are exploited by this microorganism, facilitating its persistence through evading human defense mechanisms. Among these toxins and enzymes, vacuolating toxin A (VacA) is of a great importance in the pathogenesis of H. pylori. VacA toxin shows different pattern of cytotoxicity through binding to different cell surface receptors in various cells...
2017: BioImpacts: BI
https://www.readbyqxmd.com/read/28545189/outcomes-of-a-randomized-controlled-trial-comparing-modified-high-dose-omeprazole-and-amoxicillin-triple-therapy-with-standard-triple-therapy-for-helicobacter-pylori-eradication
#2
Kitti Chunlertlith, Uaepong Limpapanasit, Pisaln Mairiang, Suda Vannaprasaht, Wichittra Tassaneeyakul, Apichat Sangchan, Kookwan Sawadpanich, Tanita Suttichaimongkol, Jamrus Pongpit, Moragot Pattarapongsin
Background: Helicobacter pylori (H. pylori) infection is related to peptic ulcer diseases and gastric cancer and eradication of H. pylori should be expected to decrease the risk of their development. Factors affecting H. pylori eradication are antibiotic resistance, CYP2C19 genotypes, drug regimen and patient compliance. Increment of omeprazole and amoxicillin dosage in clarithromycin-containing triple therapy regimen may overcome these problems and may be a better choice than the conventional clarithromycin-containing triple therapy regimen...
April 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28535789/a-multi-target-therapeutic-potential-of-prunus-domestica-gum-stabilized-nanoparticles-exhibited-prospective-anticancer-antibacterial-urease-inhibition-anti-inflammatory-and-analgesic-properties
#3
Nazar Ul Islam, Raza Amin, Muhammad Shahid, Muhammad Amin, Sumera Zaib, Jamshed Iqbal
BACKGROUND: Phytotherapeutics exhibit diverse pharmacological effects that are based on the combined action of a mixture of phytoconstituents. In this study, Prunus domestica gum-loaded, stabilized gold and silver nanoparticles (Au/Ag-NPs) were evaluated for their prospective anticancer, antibacterial, urease-inhibition, anti-inflammatory, and analgesic properties. METHODS: Au/Ag-NPs were biosynthesized and characterized with UV-Vis, FTIR, SEM, EDX, and XRD techniques...
May 23, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28531911/risk-factors-for-gastric-tumorigenesis-in-underlying-gastric-mucosal-atrophy
#4
Ji Hyun Song, Sang Gyun Kim, Eun Hyo Jin, Joo Hyun Lim, Sun Young Yang
Background/Aims: Atrophic gastritis is considered a premalignant lesion. We aimed to evaluate the risk factors for gastric tumorigenesis in underlying mucosal atrophy. Methods: A total of 10,185 subjects who underwent upper gastrointestinal endoscopy between 2003 and 2004 were enrolled in this retrospective cohort study. Follow-up endoscopy was performed between 2005 and 2014. Atrophic gastritis and intestinal metaplasia were assessed by endoscopy using the Kimura-Takemoto classification...
May 23, 2017: Gut and Liver
https://www.readbyqxmd.com/read/28531095/synthesis-of-1-2-3-triazolo-4-5-h-quinolone-derivatives-with-novel-anti-microbial-properties-against-metronidazole-resistant-helicobacter-pylori
#5
Mohammad Abu-Sini, Amal Mayyas, Nehaya Al-Karablieh, Rula Darwish, Yusuf Al-Hiari, Talal Aburjai, Shereen Arabiyat, Luay Abu-Qatouseh
Helicobacter pylori infection can lead to gastritis, peptic ulcer, and the development of mucosa associated lymphoid tissue (MALT) lymphoma. Treatment and eradication of H. pylori infection can prevent relapse and accelerate the healing of gastric and duodenal ulcers as well as regression of malignancy. Due to the increasing emergence of antibiotic resistance among clinical isolates of H. pylori, alternative approaches using newly discovered antimicrobial agents in combination with the standard antibiotic regimens for the treatment of H...
May 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28527002/design-and-development-of-amperometric-biosensor-for-the-detection-of-lead-and-mercury-ions-in-water-matrix-a-permeability-approach
#6
Manju Bhargavi Gumpu, Uma Maheswari Krishnan, John Bosco Balaguru Rayappan
Intake of water contaminated with lead (Pb(2+)) and mercury (Hg(2+)) ions leads to various toxic effects and health issues. In this context, an amperometric urease inhibition-based biosensor was developed to detect Pb(2+) and Hg(2+) ions in water matrix. The modified Pt/CeO2/urease electrode was fabricated by immobilizing CeO2 nanoparticles and urease using a semi-permeable adsorption layer of nafion. With urea as a substrate, urease catalytic activity was examined through cyclic voltammetry. Further, maximum amperometric inhibitive response of the modified Pt/CeO2/urease electrode was observed in the presence of Pb(2+) and Hg(2+) ions due to the urease inhibition at specific potentials of -0...
May 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28526368/synthesis-and-molecular-docking-study-of-some-5-6-dichloro-2-cyclopropyl-1h-benzimidazole-derivatives-bearing-triazole-oxadiazole-and-imine-functionalities-as-potent-inhibitors-of-urease
#7
Emre Menteşe, Hakan Bektaş, Bahar Bilgin Sokmen, Mustafa Emirik, Demet Çakır, Bahittin Kahveci
A new series of benzimidazole compounds including hydrazinecarbothioamide, 1,2,4-triazole, 1,3,4-oxadiazole and imine function were synthesized starting from 5,6-dichloro-2-cyclopropyl-1H-benzimidazole. All of the benzimidazole derivatives exhibited good urease inhibitor activity. Compound 6a proved to be the most potent showing an enzyme inhibitory activity with an IC50=0.06µM. Molecular docking studies were also conducted on enzyme extracted from Jack bean urease to identify the binding mode of the newly synthesized compounds...
May 10, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28522905/strategies-used-by-helicobacter-pylori-to-establish-persistent-infection
#8
REVIEW
Amin Talebi Bezmin Abadi
Helicobacter pylori (H. pylori) is a Gram-negative and motile bacterium that colonizes the hostile microniche of the human stomach, then persists for the host's entire life, if not effectively treated. Clinically, H. pylori plays a causative role in the development of a wide spectrum of diseases including chronic active gastritis, peptic ulceration, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue lymphoma. Due to the global distribution of H. pylori, it is no exaggeration to conclude that smart strategies are contributing to adaptation of the bacterium to its permanent host...
April 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28515219/a-novel-stool-pcr-test-for-helicobacter-pylori-may-predict-clarithromycin-resistance-and-patient-eradication-at-a-high-rate
#9
Erin Beckman, Ilaria Saracino, Giulia Fiorini, Courtney Clark, Vladimir Slepnev, Denise Patel, Clarissa Gomez, Reddy Ponaka, Vecheslav Elagin, Dino Vaira
Objectives: Clarithromycin-based regimens are commonly used as a first line therapy in H. pylori positive patients, however treatment failures are associated with resistance to clarithromycin. The aim of this study was to evaluate the feasibility of using stool samples to detect the presence of H. pylori DNA, while concurrently detecting mutations associated with the resistance to clarithromycin.Methods: Total DNA was extracted from 294 raw stools of H pylori positive and negative patients. TaqMan real-time PCR amplification was used to detect the presence of H...
May 17, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28512464/insights-into-the-mechanism-of-proliferation-on-the-special-microbes-mediated-by-phenolic-acids-in-the-radix-pseudostellariae-rhizosphere-under-continuous-monoculture-regimes
#10
Hongmiao Wu, Junjian Xu, Juanying Wang, Xianjin Qin, Linkun Wu, Zhicheng Li, Sheng Lin, Weiwei Lin, Quan Zhu, Muhammad U Khan, Wenxiong Lin
As potent allelochemicals, phenolic acids are believed to be associated with replanting disease and cause microflora shift and structural disorder in the rhizosphere soil of continuously monocultured Radix pseudostellariae. The transcriptome sequencing was used to reveal the mechanisms underlying the differential response of pathogenic bacterium Kosakonia sacchari and beneficial bacterium Bacillus pumilus on their interactions with phenolic acids, the main allelochemicals in root exudates of R. pseudostellariae in the monoculture system...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28507617/serum-vegf-levels-in-helicobacter-pylori-infection-and-correlation-with-helicobacter-pylori-caga-and-vaca-genes
#11
Gontar Siregar, Dina Sari, Taufik Sungkar
BACKGROUND: Helicobacter pylori vacA and cagA genes are associated with higher virulence. Vascular Endothelial Growth Factor (VEGF) is one important marker for neo-angiogenesis. AIM: The purpose of this study was to investigate the relationship between VEGF serum levels with cagA and vacA genes in H. pylori infection. METHODS: A cross-sectional study was done on eighty patients that consecutive admitted to endoscopy unit. The diagnosis of H...
April 15, 2017: Open Access Macedonian Journal of Medical Sciences
https://www.readbyqxmd.com/read/28502132/evaluation-of-non-invasive-versus-invasive-methods-for-diagnosis-of-helicobacter-pylori-infection-among-patients-with-gastroduodenal-disorders
#12
Mona A Hassan, Entsar H Ahmed, Bahaa-Eldin A Abdel-Raady, Ibrahim A Amin, Abeer ElRefaiy Refaiy, Gamal M Soliman
H. pylori, a spiral gram-negative bacterium, is associated with gastroduodenal diseases. All H. pylori diagnostic assays have limitations. Cytotoxin-associated gene A (cag A), a virulence marker, can be identified by PCR. We evaluated H. pylori diagnostic methods, invasive: rapid urease test (RUT), and histopathological examination (HE), and serology as non-invasive method. Positive cases were studied for presence of cag A gene. Upper endoscopies and gastric biopsies were performed on 67 dyspeptic patients for RUT, HE and PCR...
June 2016: Egyptian Journal of Immunology
https://www.readbyqxmd.com/read/28489837/-validation-of-the-rapid-urease-test-for-the-detection-of-helicobacter-pylori-in-a-peruvian-hospital
#13
Vladimir Espinoza Ildefonso, Harold Tabori Peinado, Claudia Meza Borja, Alejandra Bussalleu Cavero, Luis Vásquez Elera, Víctor Aguilar Sánchez, José Luis Pinto Valdivia, Jaime Cok, Alejandro Bussalleu Rivera
OBJECTIVE: To validate a rapid urease test (RUT) in Cayetano Heredia Hospital (HCH) in Lima, Peru. MATERIALS AND METHODS: This is a prospective observational study that included 181 patients over 18 years old with dyspeptic symptoms. All of them underwent upper gastrointestinal endoscopy at the Department of Gastroenterology at HCH. They had not received, during the last four weeks, proton pump inhibitors (PPIs), bismuth or antibiotics. Two biopsies of antrum were taken, one to perform the TRU (Sensibacter pylori test®) and the other one for pathology, in order to determine by both methods the presence of H...
January 2017: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/28485105/cross-regulation-of-an-artificial-metalloenzyme
#14
Yasunori Okamoto, Thomas R Ward
Cross-regulation of complex biochemical reaction networks is an essential feature of living systems. In a biomimetic spirit, we report on our efforts to program the temporal activation of an artificial metalloenzyme via cross-regulation by a natural enzyme. In the presence of urea, urease slowly releases ammonia that reversibly inhibits an artificial transfer hydrogenase. Addition of an acid, which acts as fuel, allows to maintain the system out of equilibrium.
May 9, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28483407/evaluation-of-diagnostic-accuracy-of-two-rapid-stool-antigen-tests-using-an-immunochromatographic-assay-to-detect-helicobacter-pylori
#15
Joyce Matie Kinoshita da Silva-Etto, Rejane Mattar, Cibele Aparecida Villares-Lopes, Sergio Barbosa Marques, Flair José Carrilho
OBJECTIVES: The stool antigen assay for H. pylori infection diagnosis with monoclonal antibodies is a simple and recommended technique by the Maastricht V/Florence consensus report. Recently, Pylori K-Set K-1219 (Coris Bioconcept Sprl, Belgium) and HP-F23 (Symbiosys, Brazil) have been made commercially available in Brazil. Thus, the aim of this study was to evaluate the diagnostic accuracies of these two rapid stool antigen tests by immunochromatographic assays (index tests) for the clinical practice...
May 5, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28468298/hibiscus-sabdariffa-l-and-its-bioactive-constituents-exhibit-antiviral-activity-against-hsv-2-and-anti-enzymatic-properties-against-urease-by-an-esi-ms-based-assay
#16
Sherif T S Hassan, Emil Švajdlenka, Kateřina Berchová-Bímová
For decades, Hibiscus sabdariffa L. and its phytochemicals have been shown to possess a wide range of pharmacologic properties. In this study, aqueous extract of Hibiscus sabdariffa (AEHS) and its bioactive constituent protocatechuic acid (PCA), have been evaluated in vitro for their antiviral activity against HSV-2 clinical isolates and anti-enzymatic activity against urease. Antiherpetic activity was evaluated by the titer reduction assay in infected Vero cells, and cytotoxicity was evaluated by the neutral red dye-uptake method...
April 30, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28465061/construction-of-ferrocene-modified-conducting-polymer-based-amperometric-urea-biosensor
#17
Muamer Dervisevic, Esma Dervisevic, Mehmet Senel, Emre Cevik, Huseyin Bekir Yildiz, Pınar Camurlu
Herein, an electrochemical urea sensing bio-electrode is reported that has been constructed by firstly electropolymerizing 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer (SNS-Aniline) on Pencil Graphite Electrode (PGE), then modifying the polymer coated electrode surface with di-amino-Ferrocene (DAFc) as the mediator, and lastly Urease enzyme through glutaraldehyde crosslinking. The effect of pH, temperature, polymer thickness, and applied potential on the electrode current response was investigated besides performing storage and operational stability experiments with the interference studies...
July 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28464776/gastric-mucosal-oxidative-stress-markers-in-children-with-helicobacter-pylori-infection
#18
Adel Abd Elhaleim Hagag, Saleh M Amin, Mohammed H Emara, Sally E Abo-Resha
BACKGROUND: 'Helicobacter pylori' "H. pylori" is one of the most common infections that colonizes human gastric mucosa and generates reactive oxygen and nitrogen species. The aim of this study was to evaluate the oxidative stress markers in the gastric mucosa of "H. pylori"- infected children. PATIENTS AND METHODS: The present study was carried out on 60 children infected with "H. pylori" including 28 males, 32 females with their age ranging from 7-10 years and mean age value of 8...
May 2, 2017: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/28463981/breaking-continuous-potato-cropping-with-legumes-improves-soil-microbial-communities-enzyme-activities-and-tuber-yield
#19
Shuhao Qin, Stephen Yeboah, Li Cao, Junlian Zhang, Shangli Shi, Yuhui Liu
This study was conducted to explore the changes in soil microbial populations, enzyme activity, and tuber yield under the rotation sequences of Potato-Common vetch (P-C), Potato-Black medic (P-B) and Potato-Longdong alfalfa (P-L) in a semi-arid area of China. The study also determined the effects of continuous potato cropping (without legumes) on the above mentioned soil properties and yield. The number of bacteria increased significantly (p < 0.05) under P-B rotation by 78%, 85% and 83% in the 2, 4 and 7-year continuous cropping soils, respectively compared to P-C rotation...
2017: PloS One
https://www.readbyqxmd.com/read/28463701/influence-of-root-components-of-celery-on-pyrene-bioaccessibility-soil-enzymes-and-microbial-communities-in-pyrene-and-pyrene-diesel-spiked-soils
#20
Jing Wei, Xinying Zhang, Xiaoyan Liu, Xia Liang, Xueping Chen
Though phytoremediation is deemed as a promising approach to restore polycyclic aromatic hydrocarbon (PAHs) contaminated sites, studies about how the biodegradation of PAHs is enhanced still remains incomprehensive. Effects of root components on pyrene bioaccessibility, soil enzymes and microbial communities were explored in the paper, and their interactions in simulated pyrene and pyrene-diesel spiked microcosms were tried to give a reasonable explanation. Results indicated that root components enhanced the pyrene removal of bioaccessible and adsorbed fractions by 16...
April 29, 2017: Science of the Total Environment
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