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https://www.readbyqxmd.com/read/27895127/the-pathogenic-potential-of-proteus-mirabilis-is-enhanced-by-other-uropathogens-during-polymicrobial-urinary-tract-infection
#1
Chelsie E Armbruster, Sara N Smith, Alexandra O Johnson, Valerie DeOrnellas, Kathryn A Eaton, Alejandra Yep, Lona Mody, Weisheng Wu, Harry L T Mobley
Urinary catheter use is prevalent in health care settings, and polymicrobial colonization by urease-positive organisms, such as Proteus mirabilis and Providencia stuartii, commonly occurs with long-term catheterization. We previously demonstrated that coinfection with P. mirabilis and P. stuartii increased overall urease activity in vitro and disease severity in a model of urinary tract infection (UTI). In this study, we expanded these findings to a murine model of catheter-associated UTI (CAUTI), delineated the contribution of enhanced urease activity to coinfection pathogenesis, and screened for enhanced urease activity with other common CAUTI pathogens...
November 28, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27892815/monitoring-trihalomethanes-and-nitrogenous-disinfection-by-products-in-blending-desalinated-waters-using-solid%C3%A2-phase-microextraction-and-gas-chromatography
#2
Providencia González-Hernández, Manuel Hernández-Padrón, Verónica Pino, Ana M Afonso, Juan H Ayala
A simple and efficient method has been developed for the extraction and determination of sixteen common volatile halogenated disinfection by-products (DBPs) in blending desalinated waters, using headspace solid-phase microextraction and gas chromatography with flame-ionization detection (HS-SPME/GC-FID). The DBPs determined included 4 trihalomethanes, 6 haloacetonitriles and 6 halonitromethanes. After the optimization using factorial designs of the HS-SPME parameters (optimum: carboxen/polydimethylsiloxane (CAR/PDMS) fiber, extraction time of 60 min at 30 °C, pH 7, addition of 40% (w/v) of sodium chloride, and desorption time of 2 min at 250 °C), quantification limits ranged from 3...
November 28, 2016: Environmental Technology
https://www.readbyqxmd.com/read/27876392/metallothionein-assisted-periplasmic-lead-sequestration-as-lead-sulfite-by-providencia-vermicola-strain-sj2a
#3
Jaya Sharma, Kashif Shamim, Santosh Kumar Dubey, Ram Murti Meena
Lead resistant Providencia vermicola strain SJ2A was isolated from the waste of a battery manufacturing industry which could tolerate upto 3.0mM lead nitrate in the minimal medium. Interestingly, this isolate showed presence of a plasmid borne metallothionein gene, bmtA that matched significantly (96%) with that of Pseudomonas aeruginosa. Scanning electron micrographs of bacterial cells exposed to lead revealed a unique alteration in the cell morphology from rods to long inter-connected filaments. On the other hand, electron dispersive X-ray spectroscopy (EDX) clearly indicated no significant lead adsorption therefore, we speculated intracellular sequestration in this bacterial strain...
November 19, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27865198/a-propensity-matched-case-control-study-comparing-efficacy-safety-and-costs-of-the-subcutaneous-vs-transvenous-implantable-cardioverter-defibrillator
#4
S Honarbakhsh, R Providencia, N Srinivasan, S Ahsan, M Lowe, E Rowland, R J Hunter, M Finlay, O Segal, M J Earley, A Chow, R J Schilling, P D Lambiase
BACKGROUND: Subcutaneous implantable cardioverter defibrillators (S-ICD) have become more widely available. However, comparisons with conventional transvenous ICDs (TV-ICD) are scarce. METHODS: We conducted a propensity matched case-control study including all patients that underwent S-ICD implantation over a five-year period in a single tertiary centre. Controls consisted of all TV-ICD implant patients over a contemporary time period excluding those with pacing indication, biventricular pacemakers and those with sustained monomorphic ventricular tachycardia requiring anti-tachycardia pacing...
November 7, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27863291/adenosine-guided-pulmonary-vein-isolation-versus-conventional-pulmonary-vein-isolation-in-patients-undergoing-atrial-fibrillation-ablation-an-updated-meta-analysis
#5
Nikolaos Papageorgiou, Rui Providência, Neil Srinivasan, Kostas Bronis, Francisco Moscoso Costa, Diogo Cavaco, Pedro Adragão, Dimitris Tousoulis, Ross J Hunter, Richard J Schilling, Oliver R Segal, Anthony Chow, Edward Rowland, Martin Lowe, Pier D Lambiase
BACKGROUND: Recurrent atrial fibrillation episodes following pulmonary vein isolation (PVI) are frequently due to reconnection of PVs. Adenosine can unmask dormant conduction, leading to additional ablation to improve AF-free survival. We performed a meta-analysis of the literature to assess the role of adenosine testing in patients undergoing atrial fibrillation (AF) ablation. METHODS: PubMed, EMBASE, and Cochrane databases were searched through until December 2015 for studies reporting on the role of adenosine guided-PVI versus conventional PVI in AF ablation...
November 9, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27859671/rapid-differentiation-of-cocci-mixed-bacteria-from-rods-in-voided-urine-culture-of-women-with-uncomplicated-urinary-tract-infections
#6
Chun-Chun Yang, Stephen Shei-Dei Yang, Hui-Ching Hung, I-Ni Chiang, Chiung-Hui Peng, Shang-Jen Chang
OBJECTIVES: To evaluate the ability of laser flow cytometry to predict cocci/mixed growth in the pre-analytical phase of urine specimens. METHODS: We retrospectively reviewed urine samples from women with uncomplicated urinary tract infections from urologic clinics for study. Urine analyses were performed with laser flow cytometry (UF1000i, Sysmex, Kobe, Japan) and then diagrams were generated (forward scatter vs. fluorescent light scatter). Each specimen (bacteria count >357 BACT/μL) was classified as either cocci bacteria or rods/mixed growth according to the diagrams...
November 15, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27843267/bacteriology-of-urine-specimens-obtained-from-men-with-symptomatic-benign-prostatic-hyperplasia
#7
Jude Orumuah Agbugui, E O Obarisiagbon, I I Osaigbovo
BACKGROUND: Bacteriuria and urinary tract infections are common sequelae of benign prostatic hyperplasia (BPH). Thus, the knowledge of urine bacteriology in men with symptomatic BPH in our environment may play a complementary role in management. OBJECTIVES: To determine the incidence of bacteriuria and the antibiotic sensitivity pattern of bacterial isolates in cultured urine samples of men with symptomatic BPH. PATIENTS AND METHODS: This was a 1 year prospective study...
July 2016: Nigerian Journal of Surgery: Official Publication of the Nigerian Surgical Research Society
https://www.readbyqxmd.com/read/27760824/rna-interference-directed-against-the-transglutaminase-gene-triggers-dysbiosis-of-gut-microbiota-in-drosophila
#8
Sanae Sekihara, Toshio Shibata, Mai Hyakkendani, Shun-Ichiro Kawabata
We recently reported that transglutaminase (TG) suppresses immune deficiency pathway-controlled antimicrobial peptides (IMD-AMPs), thereby conferring immune tolerance to gut microbes, and that RNAi of the TG gene in flies decreases the lifespan compared with non-TG-RNAi flies. Here, analysis of the bacterial composition of the Drosophila gut by next-generation sequencing revealed that gut microbiota comprising one dominant genus of Acetobacter in non-TG-RNAi flies was shifted to that comprising two dominant genera of Acetobacter and Providencia in TG-RNAi flies...
November 25, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27744637/bacteriological-analysis-of-saliva-from-partially-or-fully-engorged-female-adult-rhipicephalus-microplus-by-next-generation-sequencing
#9
Liangliang Xiang, Błażej Poźniak, Tian-Yin Cheng
Tick-borne diseases are a major epidemiological problem worldwide. The aim of this study was to investigate the bacterial composition of saliva obtained from engorged adult Rhipicephalus microplus females. Saliva samples collected from partially or fully engorged adult female ticks were analysed using an ultra-high-throughput Illumina HiSeq 2500 sequencing system. To elucidate the possible routes of bacterial transmission, the bacterial flora from whole ticks were also investigated. Proteobacteria, Firmicutes, and Actinobacteria were the predominant phyla in all samples, and Acinetobacter, Rickettsia, Escherichia and Coxiella were the major genera...
October 15, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27681917/highly-variable-bacterial-communities-associated-with-the-octocoral-antillogorgia-elisabethae
#10
Veronica Robertson, Brad Haltli, Erin P McCauley, David P Overy, Russell G Kerr
Antillogorgia elisabethae (synonymous with Pseudopterogorgia elisabethae) is a common branching octocoral in Caribbean reef ecosystems. A. elisabethae is a rich source of anti-inflammatory diterpenes, thus this octocoral has been the subject of numerous natural product investigations, yet relatively little is known regarding the composition, diversity and the geographic and temporal stability of its microbiome. To characterize the composition, diversity and stability of bacterial communities of Bahamian A. elisabethae populations, 17 A...
July 5, 2016: Microorganisms
https://www.readbyqxmd.com/read/27671220/acquired-resistance-to-chlorhexidine-is-it-time-to-establish-an-antiseptic-stewardship-initiative
#11
G Kampf
Chlorhexidine digluconate (CHG) is an antimicrobial agent used for different types of applications in hand hygiene, skin antisepsis, oral care, and patient washing. Increasing use raises concern regarding development of acquired bacterial resistance. Published data from clinical isolates with CHG minimum inhibitory concentrations (MICs) were reviewed and compared to epidemiological cut-off values to determine resistance. CHG resistance is rarely found in Escherichia coli, Salmonella spp., Staphylococcus aureus or coagulase-negative staphylococci...
August 26, 2016: Journal of Hospital Infection
https://www.readbyqxmd.com/read/27667823/ndm-producing-enterobacteriaceae-in-a-chinese-hospital-2014-2015-identification-of-ndm-producing-citrobacterwerkmanii-and-acquisition-of-blandm-1-carrying-plasmid-in-vivo-in-a-clinical-escherichia-coli-isolate
#12
Jingna An, Ling Guo, Lin Zhou, Yanning Ma, Yanping Luo, Chuanmin Tao, Jiyong Yang
New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae (NPE) shows prevalence in China. Little is known about the mechanisms related to the spread of NPE. Recently, a total of 51 non-duplicated NPE isolates were collected from a tertiary-care hospital in China and analysed for genetic relatedness by PFGE, antimicrobial susceptibility by Etest and sequence type by multilocus sequence typing. S1-PFGE and Southern blot analysis or PCR amplification were used for plasmid profiling. Between 2014 and 2015, 22 Escherichia coli, 10 Klebsiella pneumoniae, 9 Enterobacter cloacae, 2 Enterobacter aerogenes, 3 Providencia rettgeri, 1 Klebsiella oxytoca, 1 Proteus mirabilis, 1 Citrobacter freundii, 1 Citrobacterwerkmanii and 1 Raoultella planticola were identified as NPE...
November 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27626536/cryoballoon-or-radiofrequency-ablation-for-atrial-fibrillation
#13
LETTER
Rui Providência, Pier D Lambiase, Eloi Marijon
No abstract text is available yet for this article.
September 15, 2016: New England Journal of Medicine
https://www.readbyqxmd.com/read/27600786/bacterial-communities-associated-with-invasive-populations-of-bactrocera-dorsalis-diptera-tephritidae-in-china
#14
L J Liu, I Martinez-Sañudo, L Mazzon, C S Prabhakar, V Girolami, Y L Deng, Y Dai, Z H Li
The oriental fruit fly Bactrocera dorsalis (Hendel) is a destructive insect pest of a wide range of fruits and vegetables. This pest is an invasive species and is currently distributed in some provinces of China. To recover the symbiotic bacteria of B. dorsalis from different invasion regions in China, we researched the bacterial diversity of this fruit fly among one laboratory colony (Guangdong, China) and 15 wild populations (14 sites in China and one site in Thailand) using DNA-based approaches. The construction of 16S rRNA gene libraries allowed the identification of 24 operational taxonomic units of associated bacteria at the 3% distance level, and these were affiliated with 3 phyla, 5 families, and 13 genera...
May 31, 2016: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/27569886/prevalence-and-antimicrobial-resistance-pattern-of-bacterial-strains-isolated-from-patients-with-urinary-tract-infection-in-messalata-central-hospital-libya
#15
Mahmoud A Mohammed, Tarig M S Alnour, Osama M Shakurfo, Mariam M Aburass
OBJECTIVES: To investigate the prevalence of urinary tract infection among patients at Messalata Central Hospital, Libya, to identify the causative bacteria, and to explore their resistance pattern to antimicrobials. METHODS: A total number of 1153 urine samples were collected from patients, who attended daily to Messalata Central Hospital, Libya, in a study extended for one year. Antimicrobial susceptibility testing and isolates typing were done using Phoenix BD (BD diagnostic)...
August 2016: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27566538/ventricular-arrhythmia-burden-in-patients-with-heart-failure-and-cardiac-resynchronization-devices-the-importance-of-renal-function
#16
Girish Ganesha Babu, Matthew Webber, Rui Providencia, Sanjeev Kumar, Aerakondal Gopalamurugan, Dominic P Rogers, Holly Louise Daw, Syed Ahsan, Fakhar Khan, Anthony Chow, Martin Lowe, Edward Rowland, Pier Lambiase, Oliver R Segal
BACKGROUND: Chronic kidney disease (CKD) is a risk factor for arrhythmias in patients with heart failure (HF). However, the effects of CKD on ventricular arrhythmia (VA) burden in patients with cardiac resynchronization therapy and defibrillator (CRT-D) devices in a primary prevention setting are unknown. OBJECTIVE: To determine whether baseline CKD is associated with increased risk of VA in patients implanted with primary prevention CRT-D devices. METHODS AND RESULTS: In this retrospective study, 199 consecutive primary prevention CRT-D recipients (2005-2010) were stratified by estimated glomerular filtration rate (eGFR) levels prior to device implantation with 106 (53...
October 6, 2016: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/27561197/outcome-of-primary-prevention-implantable-cardioverter-defibrillator-therapy-according-to-new-york-heart-association-functional-classification
#17
Rui Providência, Serge Boveda, Pascal Defaye, Oliver Segal, Vincent Algalarrondo, Nicolas Sadoul, Pier Lambiase, Olivier Piot, Didier Klug, Marie-Cecile Perier, Abdeslam Bouzeman, Sergio Barra, Marie-Cécile Bories, Daniel Gras, Laurent Fauchier, Pierre Bordachar, Dominique Babuty, Jean-Claude Deharo, Christophe Leclercq, Eloi Marijon
We aimed to assess if the outcome of primary prevention implantable cardioverter defibrillators (ICDs) without cardiac resynchronization therapy is dependent on New York Heart Association (NYHA) class. Among the participants of Défibrillateur Automatique Implantable-Prévention Primaire (DAI-PP; NCT01992458) multicenter cohort study, 155 patients in NYHA class I, 504 in NYHA class II, and 188 in NYHA class III had a QRS width <120 ms and were implanted with an ICD without cardiac resynchronization therapy and, thus, were eligible for the purpose of this analysis...
October 15, 2016: American Journal of Cardiology
https://www.readbyqxmd.com/read/27561109/uniparental-ancestry-markers-in-chilean-populations
#18
Camilla Dutra Vieira-Machado, Maluah Tostes, Gabrielle Alves, Julio Nazer, Liliana Martinez, Elisabeth Wettig, Oscar Pizarro Rivadeneira, Marcela Diaz Caamaño, Jessica Larenas Ascui, Pedro Pavez, Maria da Graça Dutra, Eduardo Enrique Castilla, Ieda Maria Orioli
The presence of Native Americans, Europeans, and Africans has led to the development of a multi-ethnic, admixed population in Chile. This study aimed to contribute to the characterization of the uniparental genetic structure of three Chilean regions. Newborns from seven hospitals in Independencia, Providencia, Santiago, Curicó, Cauquenes, Valdívia, and Puerto Montt communes, belonging to the Chilean regions of Santiago, Maule, and Los Lagos, were studied. The presence of Native American mitochondrial DNA (mtDNA) haplogroups and two markers present in the non-recombinant region of the Y chromosome, DYS199 and DYS287, indicative of Native American and African ancestry, respectively, was determined...
October 2016: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/27553450/laboratory-investigation-of-a-suspected-outbreak-caused-by-providencia-stuartii-with-intermediate-resistance-to-imipenem-at-a-long-term-care-facility
#19
Yuan-Chih Mao, Chin-Lu Chang, Yhu-Chering Huang, Lin-Hui Su, Chao-Tai Lee
BACKGROUND: Providencia stuartii survives well in natural environment and often causes opportunistic infection in residents of long-term care facilities (LTCFs). Clinical isolates of P. stuartii are usually resistant to multiple antibiotics. The bacterium is also naturally resistant to colistin and tigecycline. Treatment of infections caused by carbapenem-resistant P. stuartii is challenging. METHODS: During a 15-month period in 2013-2014, four isolates (P1, P2, and P3B/P3U) of P...
July 29, 2016: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/27494198/uniparental-ancestry-markers-in-chilean-populations
#20
Camilla Dutra Vieira-Machado, Maluah Tostes, Gabrielle Alves, Julio Nazer, Liliana Martinez, Elisabeth Wettig, Oscar Pizarro Rivadeneira, Marcela Diaz Caamaño, Jessica Larenas Ascui, Pedro Pavez, Maria da Graça Dutra, Eduardo Enrique Castilla, Ieda Maria Orioli
The presence of Native Americans, Europeans, and Africans has led to the development of a multi-ethnic, admixed population in Chile. This study aimed to contribute to the characterization of the uniparental genetic structure of three Chilean regions. Newborns from seven hospitals in Independencia, Providencia, Santiago, Curicó, Cauquenes, Valdívia, and Puerto Montt communes, belonging to the Chilean regions of Santiago, Maule, and Los Lagos, were studied. The presence of Native American mitochondrial DNA (mtDNA) haplogroups and two markers present in the non-recombinant region of the Y chromosome, DYS199 and DYS287, indicative of Native American and African ancestry, respectively, was determined...
August 4, 2016: Genetics and Molecular Biology
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