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https://www.readbyqxmd.com/read/29450631/preoperative-combination-of-progressive-pneumoperitoneum-and-botulinum-toxin-type-a-in-patients-with-loss-of-domain-hernia
#1
José Bueno-Lledó, Antonio Torregrosa, Raquel Jiménez, Providencia García Pastor
INTRODUCTION: Preoperative progressive pneumoperitoneum (PPP) and botulinum toxin type A (BT) are tools in the surgical preparation of patients with loss of domain hernias (LODH). The aim of this paper is to report our experience with these preoperative techniques in 70 patients with LODH. METHODS: Observational study of 70 consecutive patients with LODH was conducted between May 2010 and May 2016. Diameters of the hernia sac, incisional hernia (VIH), and abdominal cavity (VAC) volumes, and VIH/VAC ratio were measured before and after PPP and BT, using abdominal CT scan data...
February 15, 2018: Surgical Endoscopy
https://www.readbyqxmd.com/read/29435240/gut-microbiota-in-the-burying-beetle-nicrophorus-vespilloides-provide-colonization-resistance-against-larval-bacterial-pathogens
#2
Yin Wang, Daniel E Rozen
Carrion beetles, Nicrophorus vespilloides, are reared on decomposing carrion where larvae are exposed to high populations of carcass-derived bacteria. Larvae do not become colonized with these bacteria but instead are colonized with the gut microbiome of their parents, suggesting that bacteria in the beetle microbiome outcompete the carcass-derived species for larval colonization. Here, we test this hypothesis and quantify the fitness consequences of colonization with different bacterial symbionts. First, we show that beetles colonized by their endogenous microbiome produce heavier broods than those colonized with carcass-bacteria...
February 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29430355/microbial-communities-associated-with-the-co-metabolism-of-free-cyanide-and-thiocyanate-under-alkaline-conditions
#3
Lukhanyo Mekuto, Seteno Karabo Obed Ntwampe, John Baptist N Mudumbi
This study focused on the identification of free cyanide (CDO) and thiocyanate (TDO) degrading microbial communities using a culture-dependent and independent approach. Culturable microbial species were isolated from the CDOs (n = 13) and TDOs (n = 18). The CDOs were largely dominated by Bacillus sp. while the TDOs were dominated by Bacillus sp., Klebsiella oxytoca, Providencia sp. and Pseudomonas sp. However, 16S rRNA amplicon gene-sequencing revealed the complexity and diversity of the microbial communities in contrast to the organisms that were detected using culture-dependent technique...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29414043/immuno-informatics-driven-proteome-wide-investigation-revealed-novel-peptide-based-vaccine-targets-against-emerging-multiple-drug-resistant-providencia-stuartii
#4
Yelda Asad, Sajjad Ahmad, Thanyada Rungrotmongkol, Kara E Ranaghan, Syed Sikander Azam
The bacterium Providencia stuartii, is associated with urinary tract infections and is the most common cause of purple urine bag syndrome. The increasing multi-drug resistance pattern shown by the pathogen and lack of licensed vaccines make treatment of infections caused by P. stuartii challenging. As vaccinology data against the pathogen is scarce, an in silico proteome based Reverse Vaccinology (RV) protocol, in combination with subtractive proteomics is introduced in this work to screen potential vaccine candidates against P...
January 31, 2018: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29399368/successful-treatment-of-providencia-rettgeri-cholecystitis-and-neutrophilic-cholangitis-in-a-cat
#5
Patricia L Newton, Darren R Fry
Case summary: A 15-year-old male neutered domestic shorthair cat was presented with a recent history of seizures, diarrhoea, lethargy, fever and jaundice. Marked elevation of liver enzyme activity was present and ultrasound examination was suggestive of cholecystitis and hepatitis. Neutrophilic cholangitis was confirmed on histopathology of liver biopsies. Bile culture identified a monomicrobial infection with Providencia rettgeri, which was resistant to multiple antimicrobial agents...
January 2018: JFMS Open Reports
https://www.readbyqxmd.com/read/29387810/a-novel-mapping-system-for-panoramic-mapping-of-the-left-atrium-application-to-detect-and-characterize-localized-sources-maintaining-atrial-fibrillation
#6
Shohreh Honarbakhsh, Richard J Schilling, Gurpreet Dhillon, Waqas Ullah, Emily Keating, Rui Providencia, Anthony Chow, Mark J Earley, Ross J Hunter
Objectives: This study sought to use a novel panoramic mapping system (CARTOFINDER) to detect and characterize drivers in persistent atrial fibrillation (AF). Background: Mechanisms sustaining persistent AF remain uncertain. Methods: Patients undergoing catheter ablation for persistent AF were included. A 64-pole basket catheter was used to acquire unipolar signals, which were processed by the mapping system to generate wavefront propagation maps...
January 2018: JACC. Clinical Electrophysiology
https://www.readbyqxmd.com/read/29364554/validation-of-a-novel-mapping-system-and-utility-for-mapping-complex-atrial-tachycardias
#7
S Honarbakhsh, R J Hunter, G Dhillon, W Ullah, E Keating, R Providencia, A Chow, M J Earley, R J Schilling
INTRODUCTION: This study sought to validate a novel wavefront mapping system utilizing whole-chamber basket catheters (CARTOFINDER, Biosense Webster). The system was validated in terms of (1) mapping atrial-paced beats and (2) mapping complex wavefront patterns in atrial tachycardia (AT). METHODS AND RESULTS: Patients undergoing catheter ablation for AT and persistent AF were included. A 64-pole-basket catheter was used to acquire unipolar signals that were processed by CARTOFINDER mapping system to generate dynamic wavefront propagation maps...
January 24, 2018: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/29339308/antibiotic-resistance-and-virulence-traits-of-bacterial-pathogens-from-infected-freshwater-fish-labeo-rohita
#8
Dharmaraj Ramesh, Sami Souissi
Bacterial infectious diseases are a main dangerous problem in Aquaculture farming. It causes multiple diseases in fish as well as in human being and it has considerable virulence potential. In this connection, the moot of study focus to discriminate bacterial isolates recovered from naturally diseased Labeo rohita fish and their virulent characteristics. Based on the β-haemolysis factor, four isolates (KADR11, KADR12, KADR13 and KADR14) were selected for further delineation. These bacterial isolates showed high similarity with Providencia rettgeri, Aeromonas sp...
January 12, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29334931/in-vitro-antibacterial-and-antibiotic-modifying-activity-of-crude-extract-fractions-and-3-4-7-trihydroxyflavone-from-myristica-fragrans-houtt-against-mdr-gram-negative-enteric-bacteria
#9
Joachim K Dzotam, Ingrid Konga Simo, Gabin Bitchagno, Ilhami Celik, Louis P Sandjo, Pierre Tane, Victor Kuete
BACKGROUND: Nutmeg is the seed kernel inside the fruit of Myristica fragrans Houtt. (Myristicaceae). It possesses various pharmacological activities but is used in Cameroon only for its flavor in making cakes. The present study thus aimed to investigate the in vitro antibacterial activity and antibiotic modifying activities of crude seed kernel methanol extract (MFS), fractions (MFSa-e) as well as 3',4',7-trihydroxyflavone from Myristica fragrans against a panel of multi-drug resistant (MDR) Gram-negative bacteria...
January 15, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29321302/rapid-seasonal-evolution-in-innate-immunity-of-wild-drosophila-melanogaster
#10
Emily L Behrman, Virginia M Howick, Martin Kapun, Fabian Staubach, Alan O Bergland, Dmitri A Petrov, Brian P Lazzaro, Paul S Schmidt
Understanding the rate of evolutionary change and the genetic architecture that facilitates rapid adaptation is a current challenge in evolutionary biology. Comparative studies show that genes with immune function are among the most rapidly evolving genes across a range of taxa. Here, we use immune defence in natural populations of Drosophila melanogaster to understand the rate of evolution in natural populations and the genetics underlying rapid change. We probed the immune system using the natural pathogens Enterococcus faecalis and Providencia rettgeri to measure post-infection survival and bacterial load of wild D...
January 10, 2018: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29306966/efficient-synthesis-of-%C3%AE-lactam-antibiotics-with-very-low-product-hydrolysis-by-a-mutant-providencia-rettgeri-penicillin-g-acylase
#11
Xin Pan, Li Wang, Jiajie Ye, Song Qin, Bingfang He
Penicillin G acylase (PGA) was isolated from Providencia rettgeri PX04 (PrPGApx04) and utilized for the kinetically controlled synthesis of β-lactam antibiotics. Site-directed mutagenesis was performed to increase the process efficiency. Molecular docking was carried out to speculate the key mutant positions corresponding with synthetic activity, which resulted in the achievement of an efficient mutant, βF24G. It yielded higher conversions than the wild-type enzyme in the synthesis of amoxicillin (95 versus 17...
January 6, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29305513/production-of-4-hydroxybenzoic-acid-by-an-aerobic-growth-arrested-bioprocess-using-metabolically-engineered-corynebacterium-glutamicum
#12
Yukihiro Kitade, Ryoma Hashimoto, Masako Suda, Kazumi Hiraga, Masayuki Inui
Corynebacterium glutamicum was metabolically engineered to produce 4-hydroxybenzoic acid (4-HBA), a valuable aromatic compound used as a raw material for the production of liquid crystal polymers and paraben. C. glutamicum was found to have a higher tolerance to 4-HBA toxicity than previously reported hosts used for the production of genetically engineered 4-HBA. To obtain higher titers of 4-HBA, we employed a stepwise overexpression of all seven target genes in the shikimate pathway in C. glutamicum Specifically, multiple chromosomal integrations of a mutated aroG from Escherichia coli, encoding a DAHP synthase, and wild-type aroCKB from C...
January 5, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29249433/does-presence-of-left-ventricular-contractile-reserve-improve-response-to-cardiac-resynchronization-therapy-an-updated-meta-analysis
#13
Nikolaos Papageorgiou, Rui Providência, Pier D Lambiase, Dimitris Tousoulis, Guy Lloyd, Sanjeev Bhattacharyya
BACKGROUND: Up to a third of patients undergoing cardiac resynchronization therapy (CRT) do not have a clinical or echocardiographic response. It is also unclear, whether contractile reserve (CR) could predict CRT response. This meta-analysis examines whether the presence of CR improves response to CRT and whether this is modulated by other clinical factors. METHODS: Search of PubMed/EMBASE/Cochrane databases for articles examining response to CRT stratified by the presence or not of CR...
February 1, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29243150/characterization-of-multiple-antibiotic-resistance-of-culturable-microorganisms-and-metagenomic-analysis-of-total-microbial-diversity-of-marine-fish-sold-in-retail-shops-in-mumbai-india
#14
Onkar A Naik, Ravindranath Shashidhar, Devashish Rath, Jayant R Bandekar, Archana Rath
Marine fish species were analyzed for culturable and total metagenomic microbial diversity, antibiotic resistance (AR) pattern, and horizontal gene transfer in culturable microorganisms. We observed a high AR microbial load of 3 to 4 log CFU g-1. Many fish pathogens like Providencia, Staphylococcus, Klebsiella pneumoniae, Enterobacter, Vagococcus, and Aeromonas veronii were isolated. Photobacterium and Vibrio were two major fish and human pathogens which were identified in the fish metagenome. Other pathogens that were identified were Shewanella, Acinetobacter, Psychrobacter, and Flavobacterium...
December 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29223516/antimicrobial-activity-of-plazomicin-against-enterobacteriaceae-producing-carbapenemases-from-50-brazilian-medical-centers
#15
Andreza Francisco Martins, Larissa Bail, Carmen Antonia Sanches Ito, Keite da Silva Nogueira, Tanise Vendruscolo Dalmolin, Amanda Silva Martins, Jaime Luis Lopes Rocha, Alisa W Serio, Felipe Francisco Tuon
Plazomicin is a next-generation aminoglycoside with activity against Enterobacteriaceae, including carbapenemase-producing Enterobacteriaceae (CPE). The aim of this study was to evaluate the activity of plazomicin against CPE (Klebsiella spp., Escherichia coli, Serratia spp., Enterobacter spp., Citrobacter spp., Morganella spp., Proteus spp., Providencia spp.) from different Brazilian hospitals. A total of 4000 carbapenem-resistant Enterobacteriaceae isolates were collected from clinical samples in 50 Brazilian hospitals during 2013-2015...
November 10, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29202595/biological-therapies-targeting-arrhythmias-are-cells-and-genes-the-answer
#16
Debbie Falconer, Nikolaos Papageorgiou, Emmanuel Androulakis, Yasmin Alfallouji, Wei Yao Lim, Rui Providencia, Dimitris Tousoulis
Arrhythmias can cause symptoms ranging from simple dizziness to life-threatening circulatory collapse. Current management includes medical therapy and procedures such as catheter ablation or device implantation. However, these strategies still pose a risk of serious side effects, and some patients remain symptomatic. Advancement in our understanding of how arrhythmias develop on the cellular level has made more targeted approaches possible. In addition, contemporary studies have found that several genes are involved in the pathogenesis of arrhythmias...
December 5, 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/29193200/rapid-identification-of-microorganisms-from-platelet-concentrates-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-after-short-term-incubation-on-liquid-medium
#17
Yasmine Chetouane, Gregory Dubourg, Pierre Gallian, Christophe Flaudrops, Jacques Chiaroni, Eric Chabrière, Didier Raoult, Laurence Camoin-Jau
BACKGROUND: Platelets (PLTs) are especially affected by the risk of bacterial contamination. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) is an accurate method for the routine identification of bacterial isolates in microbiology laboratories. We directly applied the MALDI-TOF method to bacterial detection in PLTs. In this study, we evaluated the sensitivity, specificity, and speed of a direct MALDI-TOF approach compared to the conventional method BACTEC. STUDY DESIGN AND METHODS: Eight bacteria associated with PLT contamination, cited by the ISBT on transfusion-transmitted infectious diseases, were spiked into PLTs for a final concentration of approximately 100 CFU/bag (n = 5 for each strain)...
November 28, 2017: Transfusion
https://www.readbyqxmd.com/read/29158999/recurrent-primary-suprahepatic-abscess-due-to-providencia-stuartii-a-rare-phenomenon
#18
Kyawzaw Lin, Aung Naing Lin, Sandar Linn, Madhavi Reddy, Anjali Bakshi
Gram-negative urease-producing bacilli, Providencia stuartii (P. stuartii), is reported in urinary tract infections, gastroenteritis, and bacteremia in humans but they rarely present with a hepatic abscess. We present a rare case of a recurrent suprahepatic cyst due to P. stuartii in a 45-year-old female, intravenous ( IV) heroin abuser with chronic hepatitis B and C. A 45-year-old female with 10 days status post right suprahepatic abscess drainage presented with recurrent, right, upper quadrant abdominal pain for one day...
September 16, 2017: Curēus
https://www.readbyqxmd.com/read/29118981/zika-virus-disease-microcephaly-and-guillain-barr%C3%A3-syndrome-in-colombia-epidemiological-situation-during-21%C3%A2-months-of-the-zika-virus-outbreak-2015-2017
#19
Nelson Méndez, Misael Oviedo-Pastrana, Salim Mattar, Isaac Caicedo-Castro, German Arrieta
Background: The Zika virus disease (ZVD) has had a huge impact on public health in Colombia for the numbers of people affected and the presentation of Guillain-Barre syndrome (GBS) and microcephaly cases associated to ZVD. Methods: A retrospective descriptive study was carried out, we analyze the epidemiological situation of ZVD and its association with microcephaly and GBS during a 21-month period, from October 2015 to June 2017. The variables studied were: (i) ZVD cases, (ii) ZVD cases in pregnant women, (iii) laboratory-confirmed ZVD in pregnant women, (iv) ZVD cases associated with microcephaly, (v) laboratory-confirmed ZVD associated with microcephaly, and (vi) ZVD associated to GBS cases...
2017: Archives of Public Health, Archives Belges de Santé Publique
https://www.readbyqxmd.com/read/29110324/cnta-oxygenase-substrate-profile-comparison-and-oxygen-dependency-of-tma-production-in-providencia-rettgeri
#20
Gints Kalnins, Eduards Sevostjanovs, Dace Hartmane, Solveiga Grinberga, Kaspars Tars
CntA oxygenase is a Rieske 2S-2Fe cluster-containing protein that has been previously described as able to produce trimethylamine (TMA) from carnitine, gamma-butyrobetaine, glycine betaine, and in one case, choline. TMA found in humans is exclusively of bacterial origin, and its metabolite, trimethylamine oxide (TMAO), has been associated with atherosclerosis and heart and renal failure. We isolated four different Rieske oxygenases and determined that there are no significant differences in their substrate panels...
November 7, 2017: Journal of Basic Microbiology
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