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https://www.readbyqxmd.com/read/28650380/high-levels-of-post-migration-hiv-acquisition-within-nine-european-countries
#1
Debora Alvarez-Del Arco, Fakoya Ibidun, Christos Thomadakis, Nikos Pantazis, Giota Touloumi, Anne-Francoise Gennotte, Freke Zuure, Henrique Barros, Cornelia Staehelin, Siri Göpel, Christoph Boesecke, Tullio Prestileo, Alain Volny-Anne, Fiona Burns, Julia Del Amo
OBJECTIVE: We aimed to estimate the proportion of post-migration HIV acquisition among HIV-positive migrants in Europe. DESIGN: To reach HIV positive migrants we designed a cross sectional study performed in HIV clinics. METHODS: The study was conducted from July 2013-July 2015 in 57 clinics (9 European countries, targeting individuals over 18 years diagnosed in the preceding 5 years and born abroad. Electronic questionnaires supplemented with clinical data were completed in any of 15 languages...
June 23, 2017: AIDS
https://www.readbyqxmd.com/read/28647748/single-level-anterior-cervical-corpectomy-and-fusion-using-a-new-3d-printed-anatomy-adaptive-titanium-mesh-cage-for-treatment-of-cervical-spondylotic-myelopathy-and-ossification-of-the-posterior-longitudinal-ligament-a-retrospective-case-series-study
#2
Teng Lu, Chao Liu, Baohui Yang, Jiantao Liu, Feng Zhang, Dong Wang, Haopeng Li, Xijing He
BACKGROUND The aim of this study was to evaluate the clinical and radiological outcomes of the use of a new 3D-printed anatomy-adaptive titanium mesh cage (AA-TMC) for single-level anterior cervical corpectomy and fusion (ACCF) in patients with cervical spondylotic myelopathy (CSM) and ossification of the posterior longitudinal ligament (OPLL). MATERIAL AND METHODS We retrospectively reviewed the records of 15 consecutive patients who underwent ACCF surgeries with AA-TMC implantation. The Japanese Orthopedic Association (JOA) scoring system, a visual analogue scale (VAS), the mean intervertebral height (MIBH) of the surgical segments, and the surgical segmental angle (SSA) were recorded preoperatively, immediately after surgery and at the final follow-up visit...
June 25, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28647155/quantifying-trade-offs-between-future-yield-levels-food-availability-and-forest-and-woodland-conservation-in-benin
#3
Confidence Duku, Sander J Zwart, Lenny G J van Bussel, Lars Hein
Meeting the dual objectives of food security and ecosystem protection is a major challenge in sub-Saharan Africa (SSA). To this end agricultural intensification is considered desirable, yet, there remain uncertainties regarding the impact of climate change on opportunities for agricultural intensification and the adequacy of intensification options given the rapid population growth. We quantify trade-offs between levels of yield gap closure, food availability and forest and woodland conservation under different scenarios...
June 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28643245/construction-and-evaluation-of-zinc-finger-nucleases
#4
Hiroshi Ochiai, Takashi Yamamoto
Zinc-finger nucleases (ZFNs) are programmable nucleases that have opened the door to the genome editing era. The construction of ZFNs recognizing a target sequence of interest is laborious, and has not been widely used recently. However, key ZFN patents are expiring over the next 2-4 years, enabling a wide range of deployments for clinical and industrial applications. This article introduces a ZFN construction protocol that uses bacterial one-hybrid (B1H) selection and single-stranded annealing (SSA) assay.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28634930/what-are-the-priorities-for-improving-cataract-surgical-outcomes-in-africa-results-of-a-delphi-exercise
#5
J C Buchan, W H Dean, A Foster, M J Burton
PURPOSE: The quality of cataract surgery delivered in sub-Saharan Africa (SSA) is a significant constraint to achieving the elimination of avoidable blindness. No published reports from routine SSA cataract services attain the WHO benchmarks for visual outcomes; poor outcomes (<6/60) often comprise 20% in published case series. This Delphi exercise aimed to identify and prioritise potential interventions for improving the quality of cataract surgery in SSA to guide research and eye health programme development...
June 20, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28632553/lumbar-retrolisthesis-in-aging-spine-what-are-the-associated-factors
#6
Feng Zhu, Hongda Bao, Zhen Liu, Zezhang Zhu, Shouyu He, Yong Qiu
STUDY DESIGN: A retrospective radiographic study. OBJECTIVE: The aim of this study is to demonstrate that lumbar retrolisthesis serves as an important compensatory mechanism and to identify the possible factor related to lumbar retrolisthesis. SUMMARY OF BACKGROUND DATA: Lumbar instability is one of the common degenerative changes, which presents as 2 radiologic features: anterolisthesis and retrolisthesis. Compared with the extensive studies on anterolisthesis, limit data are available on the characteristics and clinical relevance of lumbar retrolisthesis...
July 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28630888/gillespy-a-python-package-for-stochastic-model-building-and-simulation
#7
John H Abel, Brian Drawert, Andreas Hellander, Linda R Petzold
GillesPy is an open-source Python package for model construction and simulation of stochastic biochemical systems. GillesPy consists of a Python framework for model building and an interface to the StochKit2 suite of efficient simulation algorithms based on the Gillespie stochastic simulation algorithms (SSA). To enable intuitive model construction and seamless integration into the scientific Python stack, we present an easy to understand, action-oriented programming interface. Here, we describe the components of this package and provide a detailed example relevant to the computational biology community...
September 2016: IEEE Life Sciences Letters
https://www.readbyqxmd.com/read/28630346/the-role-of-jet-and-film-drops-in-controlling-the-mixing-state-of-submicron-sea-spray-aerosol-particles
#8
Xiaofei Wang, Grant B Deane, Kathryn A Moore, Olivia S Ryder, M Dale Stokes, Charlotte M Beall, Douglas B Collins, Mitchell V Santander, Susannah M Burrows, Camille M Sultana, Kimberly A Prather
The oceans represent a significant global source of atmospheric aerosols. Sea spray aerosol (SSA) particles comprise sea salts and organic species in varying proportions. In addition to size, the overall composition of SSA particles determines how effectively they can form cloud droplets and ice crystals. Thus, understanding the factors controlling SSA composition is critical to predicting aerosol impacts on clouds and climate. It is often assumed that submicrometer SSAs are mainly formed by film drops produced from bursting bubble-cap films, which become enriched with hydrophobic organic species contained within the sea surface microlayer...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28628838/validated-uplc-ms-ms-method-for-simultaneous-quantification-of-eight-saikosaponins-in-rat-plasma-application-to-a-comparative-pharmacokinetic-study-in-depression-rats-after-oral-administration-of-extracts-of-raw-and-vinegar-baked-bupleuri-radix
#9
Tianli Lei, Dandan Zhang, Kun Guo, Mingxiao Li, Chongning Lv, Jing Wang, Lingyun Jia, Jincai Lu
A sensitive and efficient UPLC-MS/MS method was developed and validated for simultaneous quantification of eight saikosaponins (SSa, SSb1, SSb2, SSb3, SSb4, SSc, SSd and SSf) in rat plasma. Furthermore, comparative pharmacokinetic profiles of these sakosaponins were investigated, following oral administration of extracts of raw and vinegar-baked Bupleuri Radix to depression rats. Biosamples were processed with liquid-liquid extraction technique using ethyl acetate. Chromatographic separation was accomplished on Waters BEH C18 UPLC column...
June 16, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28627675/distinctive-crypt-shape-in-a-sessile-serrated-adenoma-polyp-distribution-of-ki67-p16ink4a-wnt5a-positive-cells-and-intraepithelial-lymphocytes
#10
Kenji Hisamatsu, Kei Noguchi, Hiroyuki Tomita, Aoi Muto, Natsumi Yamada, Kazuhiro Kobayashi, Akihiro Hirata, Tomohiro Kanayama, Ayumi Niwa, Kazuhisa Ishida, Takayuki Nakashima, Yuichiro Hatano, Natsuko Suzui, Tatsuhiko Miyazaki, Akira Hara
Serrated lesions in the colorectum are currently predominantly classified as hyperplastic polyps (HPs), sessile serrated adenomas/polyps (SSA/Ps), and traditional serrated adenomas (TSAs) according to their morphology. However, the histological morphology and the molecular changes in the serrated lesions are still unclear. We performed immunohistochemistry for Ki67, p16INK4a, and WNT5A in human HPs (n=22), SSA/Ps (n=41), and TSAs (n=19). The distribution of Ki67 and p16INK4a positive cells in TSAs was different from that in HPs and SSA/Ps...
June 15, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28626076/cardiac-fibroblast-transcriptome-analyses-support-a-role-for-interferogenic-profibrotic-and-inflammatory-genes-in-anti-ssa-ro-associated-congenital-heart-block
#11
Robert M Clancy, Androo J Markham, Tanisha Jackson, Sara E Rasmussen, Miroslav Blumenberg, Jill P Buyon
The signature lesion of SSA/Ro autoantibody-associated congenital heart block (CHB) is fibrosis and a macrophage infiltrate, supporting an experimental focus on cues influencing the fibroblast component. The transcriptomes of human fetal cardiac fibroblasts were analyzed using two complementary approaches. Cardiac injury conditions were simulated in vitro by incubating human fetal cardiac fibroblasts with supernatants from macrophages transfected with the SSA/Ro-associated hY3. The top ten upregulated transcripts in the stimulated fibroblasts reflected a type I interferon (IFN) response (e...
June 16, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28619037/ifn-%C3%AE-1-with-th17-axis-cytokines-and-ifn-%C3%AE-define-different-subsets-in-systemic-lupus-erythematosus-sle
#12
Vilija Oke, Susanna Brauner, Anders Larsson, Johanna Gustafsson, Agneta Zickert, Iva Gunnarsson, Elisabet Svenungsson
BACKGROUND: Interferon (IFN)-α is thought to have a pivotal role in systemic lupus erythematosus (SLE), and type III IFNs (IFN-λ) were recently also associated with SLE. In this study, we measured levels of IFN-α, IFN-λ1, and related cytokines, such as IL-17A, IL-23, and interferon-γ-induced protein 10 (IP-10), in a Karolinska University Hospital cohort of patients with SLE and control subjects. The objective of the study was to investigate if cytokine measurements could identify different subsets of patients with active SLE and higher disease damage...
June 15, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28617023/integrated-behavioral-health-practice-facilitation-in-patient-centered-medical-homes-a-promising-application
#13
Sarah S Roderick, Nelly Burdette, Debra Hurwitz, Pano Yeracaris
INTRODUCTION: The purpose of this study was to assess the degree of behavioral health (BH) integration change in patient-centered medical homes (PCMHs) when using a practice facilitator (PF) specially trained in implementing integrated care and how a quasi-experimental design assists in this process. METHOD: Twelve PCMHs, 8 Federally Qualified Health Centers and 4 private practices, with varying degrees of BH services participated in this study. The degree of BH integration was assessed with a quasi-experimental design using the Maine Health Access Foundation's Site Self Assessment (MeHAF SSA) at baseline and after implementing site-specific BH services...
June 2017: Families, Systems & Health: the Journal of Collaborative Family Healthcare
https://www.readbyqxmd.com/read/28614199/wnt-pathway-gene-mutations-are-associated-with-the-presence-of-dysplasia-in-colorectal-sessile-serrated-adenoma-polyps
#14
Taiki Hashimoto, Satoshi Yamashita, Hiroshi Yoshida, Hirokazu Taniguchi, Toshikazu Ushijima, Tesshi Yamada, Yutaka Saito, Atsushi Ochiai, Shigeki Sekine, Nobuyoshi Hiraoka
Sessile serrated adenoma/polyps (SSA/Ps) are believed to be the major precursor of serrated pathway-derived colorectal carcinomas. To better characterize the process of progression from SSA/Ps to carcinomas, we analyzed 46 SSA/Ps with dysplasia and 45 SSA/Ps without dysplasia using targeted next-generation sequencing and immunohistochemistry. Among the WNT pathway genes analyzed, protein-truncating mutations of RNF43, APC, and ZNRF3 were identified in 23 (50%), 4 (9%), and 3 (7%) SSA/Ps with dysplasia, respectively...
June 13, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28610655/the-diagnosis-and-treatment-of-sj%C3%A3-gren-s-syndrome
#15
Ana-Luisa Stefanski, Christian Tomiak, Uwe Pleyer, Thomas Dietrich, Gerd Rüdiger Burmester, Thomas Dörner
BACKGROUND: Sjögren's syndrome is one of the more common inflammatory rheumatological diseases, with a prevalence of at least 0.4% in Germany. METHODS: This review is based on pertinent articles retrieved by a selective search in PubMed. Special attention is drawn to updated classification criteria and current treatment recommendations. RESULTS: Sjögren's syndrome has a wide variety of presentations, ranging from the local involvement of exocrine glands with keratoconjunctivitis sicca and xerostomia (the leading signs of the disease) to the systemic, extraglandular involvement of multiple organs...
May 26, 2017: Deutsches Ärzteblatt International
https://www.readbyqxmd.com/read/28609442/the-us-president-s-malaria-initiative-and-under-5-child-mortality-in-sub-saharan-africa-a-difference-in-differences-analysis
#16
Aleksandra Jakubowski, Sally C Stearns, Margaret E Kruk, Gustavo Angeles, Harsha Thirumurthy
BACKGROUND: Despite substantial financial contributions by the United States President's Malaria Initiative (PMI) since 2006, no studies have carefully assessed how this program may have affected important population-level health outcomes. We utilized multiple publicly available data sources to evaluate the association between introduction of PMI and child mortality rates in sub-Saharan Africa (SSA). METHODS AND FINDINGS: We used difference-in-differences analyses to compare trends in the primary outcome of under-5 mortality rates and secondary outcomes reflecting population coverage of malaria interventions in 19 PMI-recipient and 13 non-recipient countries between 1995 and 2014...
June 2017: PLoS Medicine
https://www.readbyqxmd.com/read/28606103/occupational-therapy-and-physiotherapy-education-and-workforce-in-anglophone-sub-saharan-africa-countries
#17
Augustine O Agho, Emmanuel B John
BACKGROUND: Sub-Saharan Africa (SSA) countries are faced with the challenge of educating a critical mass of occupational therapists (OTs) and physiotherapists (PTs) to meet the growing demand for health and rehabilitation services. The World Federation of Occupational Therapy (WFOT) and World Confederation of Physical Therapy (WCPT) have argued for the need of graduate-level training for OTs and PTs for decades. However, very few studies have been conducted to determine the availability of OT and PT training programs and practitioners in SSA countries...
June 12, 2017: Human Resources for Health
https://www.readbyqxmd.com/read/28605141/anti-ena-antibody-profiles-in-patients-with-hepatitis-c-virus-infection
#18
Christine M Litwin, Angela R Rourk
BACKGROUND: The presence of antinuclear antibodies (ANA) has been described following hepatitis C virus (HCV). Very few studies have investigated the presence of anti-extractable nuclear antigens (ENA) in HCV infection. METHODS: The aim of this study was to assess the prevalence of ENA antibodies in 100 patients with HCV infection compared to the prevalence of ENA antibodies in 100 healthy control patients. Sera from patients were tested for ENA using a multiplex microbead immunoassay...
June 12, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28602826/an-alternative-didactic-functional-and-topographic-systematization-of-the-spinal-muscles
#19
Vivianne Izabelle de Araújo Baptista, William Paganini Mayer, Ricardo Eustáquio da Silva, Ricardo Bragança de Vasconcellos Fontes, Josemberg da Silva Baptista
Back muscles are commonly described in a topographically-oriented manner without necessarily following morphological criteria. In this manner, non-standard terms may be employed which convey incorrect morphological concepts and demanding more time from both faculty and students to transmit knowledge. We propose a classification system for spinal muscles incorporating morphological concepts with the goal of facilitating knowledge transfer and suggest the term "spinal muscles". Those muscles were systematically divided and classified in seven strata from anterior to posterior: vertebro-appendicular (VA), transversarium (Tr), deep post-transversarium (DPT), middle post-transversarium (MPT), superficial post-transversarium (SPT), deep spino-appendicular (DSA) and superficial spino-appendicular (SSA)...
June 7, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28602598/adequacy-of-the-default-values-for-skin-surface-area-used-for-risk-assessment-and-french-anthropometric-data-by-a-probabilistic-approach
#20
N Dornic, A S Ficheux, A Bernard, A C Roudot
The notes of guidance for the testing of cosmetic ingredients and their safety evaluation by the Scientific Committee on Consumer Safety (SCCS) is a document dedicated to ensuring the safety of European consumers. This contains useful data for risk assessment such as default values for Skin Surface Area (SSA). A more in-depth study of anthropometric data across Europe reveals considerable variations. The default SSA value was derived from a study on the Dutch population, which is known to be one of the tallest nations in the World...
June 8, 2017: Food and Chemical Toxicology
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