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https://www.readbyqxmd.com/read/29353412/helicobacter-pylori-lps-induced-gastric-mucosal-spleen-tyrosine-kinase-syk-recruitment-to-tlr4-and-activation-occurs-with-the-involvement-of-protein-kinase-c%C3%AE
#1
B L Slomiany, A Slomiany
Spleen tyrosine kinase (Syk) has emerged recently as a major effector of proinflammatory genes expression induced by LPS-elicited TLR4 activation, and manifested by the up-amplification in the production of inflammatory mediators, including PGE2 and NO. Here, we investigated the nature of factors involved in the recruitment and interaction of Syk with TLR4 in gastric mucosa in response to H. pylori LPS. We show that stimulation of gastric mucosal cells with the LPS leads to localization of Syk with the membrane-associated TLR4 complex and its activation through phosphorylation on Tyr...
January 20, 2018: Inflammopharmacology
https://www.readbyqxmd.com/read/29353348/insulin-receptor-substrate-4-is-overexpressed-in-colorectal-cancer-and-promotes-retinoblastoma-cyclin-dependent-kinase-activation
#2
Patricia Sanmartín-Salinas, María Del Val Toledo Lobo, Fernando Noguerales-Fraguas, Miguel Toro Londoño, Antonio Jiménez-Ruiz, Luis Gonzalez Guijarro
BACKGROUND: Insulin receptor substrate 4 (IRS-4) is an adaptor protein for which new evidence suggests plays a role in tumour promotion. METHODS: We described nuclear IRS-4 in RKO colon cancer cell lines in biopsies of patients with colorectal cancer (CRC) (n = 20) and in matched adjacent normal colorectal (MANC) tissue (n = 20). RESULTS: Treatment with physiological doses of IGF-1 promoted nuclear influx of IRS-4 from cellular cytosol in RKO cells...
January 20, 2018: Journal of Gastroenterology
https://www.readbyqxmd.com/read/29353324/stromalized-microreactor-supports-murine-hematopoietic-progenitor-enrichment
#3
Danika Khong, Matthew Li, Amy Singleton, Ling-Yee Chin, Biju Parekkadan
There is an emerging need to process, expand, and even genetically engineer hematopoietic stem and progenitor cells (HSPCs) prior to administration for blood reconstitution therapy. A closed-system and automated solution for ex vivo HSC processing can improve adoption and standardize processing techniques. Here, we report a recirculating flow bioreactor where HSCs are stabilized and enriched for short-term processing by indirect fibroblast feeder coculture. Mouse 3 T3 fibroblasts were seeded on the extraluminal membrane surface of a hollow fiber micro-bioreactor and were found to support HSPC cell number compared to unsupported BMCs...
January 20, 2018: Biomedical Microdevices
https://www.readbyqxmd.com/read/29353271/erythrocytes-and-skeletal-muscle-unsaturated-and-omega-6-fatty-acids-are-positively-correlated-after-caloric-restriction-and-exercise
#4
Miquel Martorell, Victoria Pons, Joan Carles Domingo, Xavier Capó, Antoni Sureda, Franchek Drobnic, Josep-Antoni Tur, Antoni Pons
BACKGROUND: Nutritional intervention studies with fatty acid (FA) supplements assess the efficacy of the intervention by measuring the changes in erythrocyte membrane lipid profiles reflected in tissue composition changes. The aim was to determine the effects of caloric restriction (CR) on erythrocytes lipid composition and to compare and correlate these changes with skeletal muscle acid profiles after CR. METHODS: Erythrocytes were obtained from 11 healthy men before and after 4 weeks of 33% CR in post-exercise conditions; muscle biopsies were obtained from the same athletes after 4 weeks of 33% CR in post-exercise conditions...
January 19, 2018: Annals of Nutrition & Metabolism
https://www.readbyqxmd.com/read/29353240/pip4k2a-regulates-intracellular-cholesterol-transport-through-modulating-pi-4-5-p2-homeostasis
#5
Ao Hu, Xue-Tong Zhao, Heng Tu, Ting Xiao, Ting Fu, Yan Wang, Yong Liu, Xiong-Jie Shi, Jie Luo, Bao-Liang Song
The transport of LDL-derived cholesterol from lysosomes to peroxisomes is mediated by membrane contacts, which are facilitated by the lysosomal protein synaptotagmin VII and the peroxisomal lipid phosphatidylinositol 4, 5-bisphosphate (PI(4,5)P2). Here, we used RNA interference to search for regulators of PI(4,5)P2 and to study the effects of altered PI(4,5)P2 homeostasis on cholesterol transport. We found that knockdown of phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) reduced peroxisomal PI(4,5)P2 levels, decreased lysosome-peroxisome membrane contacts, and increased accumulation of lysosomal cholesterol in human SV-589 fibroblasts...
January 20, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29353198/a-tiered-approach-to-assess-effects-of-diclofenac-on-the-brown-mussel-perna-perna-a-contribution-to-characterize-the-hazard
#6
Mayana Karoline Fontes, Paloma Kachel Gusso-Choueri, Luciane Alves Maranho, Denis Moledo de Souza Abessa, Wesley Almeida Mazur, Bruno Galvão de Campos, Luciana Lopes Guimarães, Marcos Sergio de Toledo, Daniel Lebre, Joyce Rodrigues Marques, Andreia Arantes Felicio, Augusto Cesar, Eduardo Alves Almeida, Camilo Dias Seabra Pereira
Pharmaceutical discharges into the aquatic ecosystem are of environmental concern and sewage treatment plants (STPs) have been pointed out as the major source of these compounds to coastal zones, where oceanic disposal of sewage occurs through submarine outfalls. Diclofenac (DCF) is one of the most frequently detected pharmaceuticals in water, but little is known about the effects on marine organisms. In this study, we employed a tiered approach involving the determination of environmental concentrations of DCF in marine water and the adverse biological effects for fertilization, embryo-larval development and biomarker responses of the mussel Perna perna...
December 29, 2017: Water Research
https://www.readbyqxmd.com/read/29353192/monte-carlo-calculations-of-the-cellular-s-values-for-%C3%AE-particle-emitting-radionuclides-incorporated-into-the-nuclei-of-cancer-cells-of-the-mda-mb231-mcf7-and-pc3-lines
#7
E L Rojas-Calderón, O Ávila, G Ferro-Flores
S-values (dose per unit of cumulated activity) for alpha particle-emitting radionuclides and monoenergetic alpha sources placed in the nuclei of three cancer cell models (MCF7, MDA-MB231 breast cancer cells and PC3 prostate cancer cells) were obtained by Monte Carlo simulation. The MCNPX code was used to calculate the fraction of energy deposited in the subcellular compartments due to the alpha sources in order to obtain the S-values. A comparison with internationally accepted S-values reported by the MIRD Cellular Committee for alpha sources in three sizes of spherical cells was also performed leading to an agreement within 4% when an alpha extended source uniformly distributed in the nucleus is simulated...
January 10, 2018: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/29353162/monolayers-of-a-tetrazine-containing-gemini-amphiphile-interplays-with-biomembrane-lipids
#8
Hiromichi Nakahara, Masayori Hagimori, Takahiro Mukai, Osamu Shibata
The property of a newly synthesized tetrazine derivative comprised of double C18-saturated hydrocarbon chain (C18-rTz-C18) has been studied in situ at the air-water interface. C18-rTz-C18 or a gemini amphiphile contributes to restriction of its tetrazine moiety on the interface, which is expected to be used for bioimaging and analytical reagents. Herein, to understand lateral interactions between Tz and biomembrane constituents, we investigated the interfacial behavior of Langmuir monolayers composed of C18-rTz-C18 and biomembrane lipids such as DPPC, DPPG, DPPE, PSM, and Cholesterol (Ch)...
January 17, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29353147/prediction-of-conserved-sites-and-domains-in-glycoproteins-b-c-and-d-of-herpes-viruses
#9
Muhammad Asif Rasheed, Abdur Rahman Ansari, Awais Ihsan, Muhammad Tariq Navid, Shahid Ur-Rehman, Sohail Raza
Glycoprotein B (gB), C (gC) and D (gD) of herpes simplex virus are implicated in virus adsorption and penetration. The gB, gC and gD are glycoproteins for different processes of virus binding and attachment to the host cells. Moreover, their expression is necessary and sufficient to induce cell fusion in the absence of other glycoproteins. Egress of herpes simplex virus (HSV) and other herpes viruses from cells involves extensive modification of cellular membranes and sequential envelopment, de-envelopment and re-envelopment steps...
January 17, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29353142/mirna-185-regulates-the-vegfa-signaling-pathway-in-dairy-cows-with-retained-fetal-membranes
#10
REVIEW
C Y Zheng, X Zou, H J Lin, B C Zhao, M L Zhang, C H Luo, S X Fu
Retention of fetal membranes (RFM) of cows is an important reproductive disturbance, and is related to miRNAs. Vascular endothelial growth factor (VEGF)A, regulated by miRNA-185, can activate arachidonic acid (ARA) release via the VEGFA signaling pathway, which influences RFM. The aim of this study was to explore the pathogenic mechanism of RFM by investigating the regulatory relationship between miRNA-185 and the VEGFA signaling pathway. Serum samples of healthy Holstein dairy cows (n = 20) and RFM cows (n = 12), with a similar age, parity, weight, and milk yield, were collected to detect VEGFA and ARA concentrations at 6, 12, and 24 h after calving...
January 9, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353141/vitrification-of-testicular-tissue-from-prepubertal-cats-in-cryotubes-using-different-cryoprotectant-associations
#11
David Baruc Cruvinel Lima, Ticiana Franco Pereira da Silva, Annice Aquino-Cortez, Johanna Leiva-Revilla, Lúcia Daniel Machado da Silva
Protocols for the cryopreservation of testicular tissue are not yet established. In cats, few studies have been conducted on testicular vitrification using different cryoprotectant associations (CPAs). Thus, the objective of this study was to compare the effect of different CPAs on the vitrification of testicular tissue from prepubertal cats in cryotubes. We used 10 pairs of testicles, with each pair divided into 8 fragments that were distributed into different experimental groups. Two of these fragments were allocated into the control group (CG) and the other six were distributed according to the CPAs to be tested (dimethyl sulfoxide (DMSO)/glycerol (GLY), ethylene glycol (EG)/GLY, or DMSO/EG)...
January 5, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353140/development-of-sea-bream-sparus-aurata-semen-vitrification-protocols
#12
Loredana Zilli, Annalisa Bianchi, Maroua Sabbah, Laura Pecoraro, Roberta Schiavone, Sebastiano Vilella
The long-term goal of this research project is to set up efficient protocol that can be used to develop a standardized approach for vitrification of marine fish spermatozoa. In particular, the aim of the present study was to develop a vitrification protocol for sea bream (Sparus aurata) spermatozoa. To draw up the protocol, we tested two different dilution media (1% NaCl and Mounib medium), three different vitrification devices (loops, drops and cut straws), different cryoprotectants (CPs) and three different equilibration times (30, 60 and 120 s)...
January 11, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353012/fabrication-of-poly-ethylene-glycol-coated-mesoporous-nanocomposite-zno-fe2o3-for-methotrexate-delivery-an-integrated-nanoplatform-for-dual-mode-cancer-therapy
#13
Azam S Alavi, Azadeh Meshkini
Although chemotherapy is an effective strategy for treatment of tumor cells, the non-specific distribution of chemotherapy drugs and their poor aqueous solubility result in impaired treatment and cause serious side effects in patients. In this study, mesoporous ZnO@Fe2O3 nanocomposite was fabricated and used as a platform for drug delivery. ZnO@Fe2O3 nanocomposite showed a high capacity for the adsorption of chemotherapy drug, methotrexate (MTX). Drug-loaded nanoparticle was coated with poly(ethylene glycol) (PEG), leading to the enhanced colloidal stability and good cytocompatibility...
January 15, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29353007/nitric-oxide-suppresses-aluminum-induced-programmed-cell-death-in-peanut-arachis-hypoganea-l-root-tips-by-improving-mitochondrial-physiological-properties
#14
Huyi He, Wenjing Huang, Thet Lwin Oo, Minghua Gu, Jie Zhan, Aiqin Wang, Long-Fei He
Aluminum (Al) stress alters nitric oxide (NO) and induces programmed cell death (PCD) in plants. Recent study has shown that NO inhibits Al-induced PCD. However, the mechanism of NO inhibiting Al-induced PCD has not been revealed yet. Here, we investigated the behavior of mitochondria during Al-induced PCD suppressed by NO in peanut. Seedlings of peanut was grown hydroponically in a controllable growth room. The mitochondrial physiological parameters were determined spectrophotometrically. The expression of AhANT and AhHsp70 was determined by quantitative RT-PCR...
January 15, 2018: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29353001/membrane-progesterone-receptors-%C3%AE-and-%C3%AE-have-potential-as-prognostic-biomarkers-of-endometrial-cancer
#15
Maša Sinreih, Tamara Knific, Peter Thomas, Snježana Frković Grazio, Tea Lanišnik Rižner
Endometrial cancer (EC) is one of the most common malignancies in women worldwide. EC is linked to chronic exposure to estrogens that is unopposed by protective effects of progesterone. Progesterone modulates gene expression via classical nuclear receptors, and has rapid effects via the less characterized membrane-bound progesterone receptors (mPRs) of the progestin and adipoQ receptor (PAQR) family. The presence of mPRs in EC has not been investigated to date. The aims of this study were to examine PAQR7, PAQR8 and PAQR5, which encode for mPRα, mPRβ and mPRγ, respectively, for their expression and localization in EC tissue and adjacent control endometrium...
January 15, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29352993/comparison-of-chorioretinal-layers-in-rhesus-macaques-using-spectral-domain-optical-coherence-tomography-and-high-resolution-histological-sections
#16
Glenn Yiu, Zhe Wang, Christian Munevar, Eric Tieu, Bradley Shibata, Brittany Wong, David Cunefare, Sina Farsiu, Jeffrey Roberts, Sara Thomasy
Nonhuman primates are important preclinical models of retinal diseases because they uniquely possess a macula similar to humans. Ocular imaging technologies such as spectral-domain optical coherence tomography (SD-OCT) allow noninvasive, in vivo measurements of chorioretinal layers with near-histological resolution. However, the boundaries are based on differences in reflectivity, and detailed correlations with histological tissue layers have not been explored in rhesus macaques, which are widely used for biomedical research...
January 15, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29352992/endobronchial-granular-tumor-excision-with-bronchial-resection-inclusive-of-second-carinoplasty
#17
Hironori Ishibashi, Shunichi Baba, Yasuhiro Nakashima, Chihiro Takasaki, Masashi Kobayashi, Kenichi Okubo
Bronchial granular cell tumor is a rare tumor. A 39-year-old non-smoker woman was admitted to our hospital with back pain. Chest CT and bronchoscopy scan revealed a 10mm endobronchial tumor arising from the membrane near the second carina. The tumor was excised inclusive of the right second carina containing the extraluminally invaded endobronchial mass and was diagnosed as a granular cell tumor. The postoperative course was uneventful and the patient was well without signs of recurrence for 3 year follow-up...
January 15, 2018: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29352979/evaluation-of-sheep-ovarian-tissue-cryopreserved-by-slow-freezing-or-vitrificaton-after-chick-embryo-chorioallantoic-membrane-transplantation
#18
Mahboubeh Vatanparast, Mohammad Ali Khalili, Nahid Yari, Marjan Omidi, Mehdi Mohsenzadeh
The aim of our investigations was to compare the effectiveness of two methods for cryopreservation of sheep ovarian tissue, slow freezing and vitrification. The quality of cryopreserved tissues was evaluated after 5 days of thawing and chorioallantoic membrane (CAM) transplantation. Follicular structure, stromal integrity and neovascularization were assessed. The areas of fibrosis and necrosis were measured using MICROVISIBLE software, and proliferation was assessed with Ki-67 immunostaning. After 5 days of culture, the proportion of primordial follicles decreased, whereas the primary and intermediary follicles increased insignificantly (p > ...
January 15, 2018: Cryobiology
https://www.readbyqxmd.com/read/29352922/cellulose-nanofiber-embedded-sulfonated-poly-ether-sulfone-membranes-for-proton-exchange-membrane-fuel-cells
#19
Xianlin Xu, Rui Li, Chenxiao Tang, Hang Wang, Xupin Zhuang, Ya Liu, Weimin Kang, Lei Shi
Cellulose nanofibers were embedded into sulfonated poly (ether sulfone) matrix to heighten the water retention and proton conductivity of proton exchange membranes (PEMs). Cellulose nanofibers were obtained by hydrolyzing cellulose acetate nanofibers, which were prepared via electrostatic-induction-assisted solution blow spinning. Morphology, thermal stability, and mechanical properties of the PEMs were investigated. The results showed that proton conductivity, water uptake, and methanol permeability of the composite membranes were improved...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352896/biological-activities-and-pharmaceutical-applications-of-polysaccharide-from-natural-resources-a-review
#20
REVIEW
Yue Yu, Mingyue Shen, Qianqian Song, Jianhua Xie
Pharmacotherapy using natural substances can be currently regarded as a very promising future alternative to conventional therapy. As biological macromolecules, polysaccharide together with protein and polynucleotide, are extremely important biomacromoleules which play important roles in the growth and development of living organism. Polysaccharide is important component of higher plants, membrane of the animal cell and the cell wall of microbes. It is also closely related to the physiological functions. Recently, increasing attention has been paid on polysaccharides as an important class of bioactive natural products...
March 1, 2018: Carbohydrate Polymers
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