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https://www.readbyqxmd.com/read/28813673/multipotent-basal-stem-cells-maintained-in-localized-proximal-niches-support-directed-long-ranging-epithelial-flows-in-human-prostates
#1
Mohammad Moad, Edouard Hannezo, Simon J Buczacki, Laura Wilson, Amira El-Sherif, David Sims, Robert Pickard, Nicholas A Wright, Stuart C Williamson, Doug M Turnbull, Robert W Taylor, Laura Greaves, Craig N Robson, Benjamin D Simons, Rakesh Heer
Sporadic mitochondrial DNA mutations serve as clonal marks providing access to the identity and lineage potential of stem cells within human tissues. By combining quantitative clonal mapping with 3D reconstruction of adult human prostates, we show that multipotent basal stem cells, confined to discrete niches in juxta-urethral ducts, generate bipotent basal progenitors in directed epithelial migration streams. Basal progenitors are then dispersed throughout the entire glandular network, dividing and differentiating to replenish the loss of apoptotic luminal cells...
August 15, 2017: Cell Reports
https://www.readbyqxmd.com/read/28811371/angular-reconstitution-based-3d-reconstructions-of-nanomolecular-structures-from-superresolution-light-microscopy-images
#2
Desirée Salas, Antoine Le Gall, Jean-Bernard Fiche, Alessandro Valeri, Yonggang Ke, Patrick Bron, Gaetan Bellot, Marcelo Nollmann
Superresolution light microscopy allows the imaging of labeled supramolecular assemblies at a resolution surpassing the classical diffraction limit. A serious limitation of the superresolution approach is sample heterogeneity and the stochastic character of the labeling procedure. To increase the reproducibility and the resolution of the superresolution results, we apply multivariate statistical analysis methods and 3D reconstruction approaches originally developed for cryogenic electron microscopy of single particles...
August 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28810253/outcomes-of-self-expanding-metal-stents-in-malignant-colonic-obstruction-are-independent-of-location-or-length-of-the-stenosis-results-of-a-retrospective-single-center-series
#3
Paul K Schoonbeek, Pim Genzel, Eline H van den Berg, O Aart van Dobbenburgh, Frank Ter Borg
AIM: To evaluate the length and location of stenosis in the colon as predictors of technical and clinical outcomes of stent placement in patients presenting with obstructive colorectal cancer. METHODS: A prospective single-center cohort study of patients treated with a colonic stent for malignant obstruction, regardless of stenosis length or location. Stenosis length was assessed globally on the appropriate CT slice as well as by 3D CT reconstruction. We analyzed whether outcomes were different in patients with a right sided-tumor and/or a stenosis >4 cm long...
August 16, 2017: Digestive Surgery
https://www.readbyqxmd.com/read/28809874/three-dimensional-object-visualization-and-detection-in-low-light-illumination-using-integral-imaging
#4
Adam Markman, Xin Shen, Bahram Javidi
Conventional two-dimensional (2D) imaging systems that operate in the visible spectrum may perform poorly in environments under low light illumination. In this work, we present the potential of passive three-dimensional (3D) integral imaging (II) to perform 3D imaging of a scene under low light conditions in the visible spectrum and without the need for a photon counting or cooled CCD camera. Using dedicated algorithms, we demonstrate that the reconstructed 3D integral image is naturally optimum in a maximum likelihood sense in low light levels and in the presence of detector noise enabling object visualization in the scene...
August 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28809787/indoor-scene-point-cloud-registration-algorithm-based-on-rgb-d-camera-calibration
#5
Chi-Yi Tsai, Chih-Hung Huang
With the increasing popularity of RGB-depth (RGB-D) sensor, research on the use of RGB-D sensors to reconstruct three-dimensional (3D) indoor scenes has gained more and more attention. In this paper, an automatic point cloud registration algorithm is proposed to efficiently handle the task of 3D indoor scene reconstruction using pan-tilt platforms on a fixed position. The proposed algorithm aims to align multiple point clouds using extrinsic parameters of the RGB-D camera obtained from every preset pan-tilt control point...
August 15, 2017: Sensors
https://www.readbyqxmd.com/read/28809471/3d-analysis-of-fuel-cell-electrocatalyst-degradation-on-alternate-carbon-supports
#6
Brian T Sneed, David A Cullen, Kimberly S Reeves, Ondrej E Dyck, David A Langlois, Rangachary Mukundan, Rodney L Borup, Karren L More
Understanding the mechanisms associated with Pt/C electrocatalyst degradation in proton exchange membrane fuel cell (PEMFC) cathodes is critical for the future development of higher-performing materials; however, there is a lack of information regarding Pt coarsening under PEMFC operating conditions within the cathode catalyst layer. We report a direct and quantitative 3D study of Pt dispersions on carbon supports (high surface area carbon (HSAC), Vulcan XC-72, and graphitized carbon) with varied surface areas, graphitic character, and Pt loadings ranging from 5-40 wt...
August 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28808803/a-prototype-educational-model-for-hepatobiliary-interventions-unveiling-the-role-of-graphic-designers-in-medical-3d-printing
#7
Ramin Javan, Merissa N Zeman
In the context of medical three-dimensional (3D) printing, in addition to 3D reconstruction from cross-sectional imaging, graphic design plays a role in developing and/or enhancing 3D-printed models. A custom prototype modular 3D model of the liver was graphically designed depicting segmental anatomy of the parenchyma containing color-coded hepatic vasculature and biliary tree. Subsequently, 3D printing was performed using transparent resin for the surface of the liver and polyamide material to develop hollow internal structures that allow for passage of catheters and wires...
August 14, 2017: Journal of Digital Imaging: the Official Journal of the Society for Computer Applications in Radiology
https://www.readbyqxmd.com/read/28808754/three-dimensionalct-analysis-of-femoral-tunnel-position-after-acl-reconstruction-a-prospective-study-of-one-hundred-and-thirty-five-cases
#8
Olivier Reynaud, Cécile Batailler, Timothy Lording, Sebastien Lustig, Elvire Servien, Philippe Neyret
BACKGROUND: One of the principal causes for failure of anterior cruciate ligament reconstruction (ACL) is femoral tunnel mal-position. Several studies compare the position of femoral tunnels achieved with various techniques, with small series and using a quadrant assessment method. QUESTIONS: (1) What is the incidence of anatomical positioning of the intra-articular femoral tunnel aperture in primary ACL reconstruction in a university knee surgery? (2) What are the main errors in positioning? METHODS: 3D-CT scans were performed after primary ACL reconstruction in 135 consecutive cases...
August 14, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28808032/mechanical-constraint-from-growing-jaw-facilitates-mammalian-dental-diversity
#9
Elodie Renvoisé, Kathryn D Kavanagh, Vincent Lazzari, Teemu J Häkkinen, Ritva Rice, Sophie Pantalacci, Isaac Salazar-Ciudad, Jukka Jernvall
Much of the basic information about individual organ development comes from studies using model species. Whereas conservation of gene regulatory networks across higher taxa supports generalizations made from a limited number of species, generality of mechanistic inferences remains to be tested in tissue culture systems. Here, using mammalian tooth explants cultured in isolation, we investigate self-regulation of patterning by comparing developing molars of the mouse, the model species of mammalian research, and the bank vole...
August 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28807205/facilitating-surgeon-understanding-of-complex-anatomy-using-a-three-dimensional-printed-model
#10
Barrett P Cromeens, William C Ray, Brad Hoehne, Fikir Abayneh, Brent Adler, Gail E Besner
BACKGROUND: 3-dimensional prints (3DP) anecdotally facilitate surgeon understanding of anatomy and decision-making. However, the actual benefit to surgeons or patients has not been quantified. This study investigates how surgeon understanding of complex anatomy is altered by a 3DP compared to computed tomography (CT) scan or CT + digital reconstruction (CT + DR). MATERIALS AND METHODS: Key anatomic features were segmented from a CT-abdomen/pelvis of pygopagus twins to build a DR and printed in color on a 3D printer...
August 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28806773/quantitative-accuracy-of-177lu-spect-imaging-for-molecular-radiotherapy
#11
Emilio Mezzenga, Vincenzo D'Errico, Marco D'Arienzo, Lidia Strigari, Koutla Panagiota, Federica Matteucci, Stefano Severi, Giovanni Paganelli, Andrew Fenwick, David Bianchini, Francesco Marcocci, Anna Sarnelli
The purpose of this study is to investigate the optimal reference geometry for gamma camera calibration. Yet another question of interest was to assess the influence of the number of 3D Ordered Subsets Expectation Maximization (3D-OSEM) updates on activity quantification for SPECT imaging with 177Lu. The accuracy of 177Lu activity quantification was assessed both in small and in large objects. Two different reference geometries, namely a cylindrical homogeneous phantom and a Jaszczak 16 ml sphere surrounded by cold water, were used to determine the gamma camera calibration factor of a commercial SPECT/CT system...
2017: PloS One
https://www.readbyqxmd.com/read/28806424/geometric-evaluation-of-the-effect-of-prosthetic-rehabilitation-on-the-facial-appearance-of-mandibulectomy-patients-a-preliminary-study
#12
Amel M Aswehlee, Mahmoud E Elbashti, Mariko Hattori, Yuka I Sumita, Hisashi Taniguchi
PURPOSE: The purpose of this study was to geometrically evaluate the effect of prosthetic rehabilitation on the facial appearance of mandibulectomy patients. MATERIALS AND METHODS: Facial scans (with and without prostheses) were performed for 16 mandibulectomy patients using a noncontact three-dimensional (3D) digitizer, and 3D images were reconstructed with the corresponding software. The 3D datasets were geometrically evaluated and compared using 3D evaluation software...
August 14, 2017: International Journal of Prosthodontics
https://www.readbyqxmd.com/read/28804569/dynamic-registration-for-gigapixel-serial-whole-slide-images
#13
Blair J Rossetti, Fusheng Wang, Pengyue Zhang, George Teodoro, Daniel J Brat, Jun Kong
High-throughput serial histology imaging provides a new avenue for the routine study of micro-anatomical structures in a 3D space. However, the emergence of serial whole slide imaging poses a new registration challenge, as the gigapixel image size precludes the direct application of conventional registration techniques. In this paper, we develop a three-stage registration with multi-resolution mapping and propagation method to dynamically produce registered subvolumes from serial whole slide images. We validate our algorithm with gigapixel images of serial brain tumor sections and synthetic image volumes...
April 2017: Proceedings of the IEEE International Symposium on Biomedical Imaging: from Nano to Macro
https://www.readbyqxmd.com/read/28803692/a-janus-faced-im30-ring-involved-in-thylakoid-membrane-fusion-is-assembled-from-im30-tetramers
#14
Michael Saur, Raoul Hennig, Phoebe Young, Kristiane Rusitzka, Nadja Hellmann, Jennifer Heidrich, Nina Morgner, Jürgen Markl, Dirk Schneider
Biogenesis and dynamics of thylakoid membranes likely involves membrane fusion events. Membrane attachment of the inner membrane-associated protein of 30 kDa (IM30) affects the structure of the lipid bilayer, finally resulting in membrane fusion. Yet, how IM30 triggers membrane fusion is largely unclear. IM30 monomers pre-assemble into stable tetrameric building blocks, which further align to form oligomeric ring structures, and differently sized IM30 rings bind to membranes. Based on a 3D reconstruction of IM30 rings, we locate the IM30 loop 2 region at the bottom of the ring and show intact membrane binding but missing fusogenic activity of loop 2 mutants...
August 2, 2017: Structure
https://www.readbyqxmd.com/read/28803216/3d-2d-registration-with-superabundant-vessel-reconstruction-for-cardiac-resynchronization-therapy
#15
Daniel Toth, Maria Panayiotou, Alexander Brost, Jonathan M Behar, Christopher A Rinaldi, Kawal S Rhode, Peter Mountney
A key component of image guided interventions is the registration of preoperative and intraoperative images. Classical registration approaches rely on cross-modality information; however, in modalities such as MRI and X-ray there may not be sufficient cross-modality information. This paper proposes a fundamentally different registration approach which uses adjacent anatomical structures with superabundant vessel reconstruction and dynamic outlier rejection. In the targeted clinical scenario of cardiac resynchronization therapy (CRT) delivery, preoperative, non contrast-enhanced, MRI is registered to intraoperative, contrasted X-ray fluoroscopy...
August 5, 2017: Medical Image Analysis
https://www.readbyqxmd.com/read/28801918/motion-compensation-in-the-region-of-the-coronary-arteries-based-on-partial-angle-reconstructions-from-short-scan-ct-data
#16
Juliane Hahn, Herbert Bruder, Christopher Rohkohl, Thomas Allmendinger, Karl Stierstorfer, Thomas Flohr, Marc Kachelrieß
PURPOSE: In order to mitigate motion-induced artifacts, several motion compensation (MoCo) methods have been developed, which are either able to a) compensate for severe artifacts, but utilize the data for the reconstruction of several cardiac phases, or b) improve image quality of a single reconstruction with only moderate motion artifacts. We propose a method combining both benefits: dose efficiency by utilizing only the data needed for a single short-scan reconstruction while still being able to compensate for severe artifacts...
August 12, 2017: Medical Physics
https://www.readbyqxmd.com/read/28801800/the-horizontal-plane-appearances-of-scoliosis-what-information-can-be-obtained-from-top-view-images
#17
REVIEW
Tamás S Illés, Máté Burkus, Szabolcs Somoskeőy, Fabien Lauer, Francois Lavaste, Jean F Dubousset
PURPOSE: A posterior-anterior vertebral vector is proposed to facilitate visualization and understanding of scoliosis. The aim of this study was to highlight the interest of using vertebral vectors, especially in the horizontal plane, in clinical practice. METHODS: We used an EOS two-/three-dimensional (2D/3D) system and its sterEOS 3D software for 3D reconstruction of 139 normal and 814 scoliotic spines-of which 95 cases were analyzed pre-operatively and post-operatively, as well...
August 11, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28801298/-application-of-3d-visualization-technique-in-breast-cancer-surgery-with-immediate-breast-reconstruction-using-laparoscopically-harvested-pedicled-latissimus-dorsi-muscle-flap
#18
Pu-Sheng Zhang, Li-Kun Wang, Yun-Feng Luo, Fu-Jun Shi, Lin-Yun He, Cheng-Bing Zeng, Yu Zhang, Chi-Hua Fang
OBJECTIVE: To study the value of 3D visualization technique in breast-preserving surgery for breast cancer with immediate breast reconstruction using laparoscopically harvested pedicled latissimus dorsi muscle flap. METHODS: From January, 2015 to May, 2016, 30 patients with breast cancer underwent breast-preserving surgery with immediate breast reconstruction using pedicled latissimus dorsi muscle flap. The CT data of the arterial phase and venous phase were collected preoperatively and imported into the self-developed medical image 3D visualization system for image segmentation and 3D reconstruction...
August 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28800252/progressive-changes-in-walking-kinematics-and-kinetics-after-anterior-cruciate-ligament-injury-and-reconstruction-a-review-and-meta-analysis
#19
Lindsay V Slater, Joseph M Hart, Adam R Kelly, Christopher M Kuenze
CONTEXT:   Anterior cruciate ligament (ACL) injury and ACL reconstruction (ACLR) result in persistent alterations in lower extremity movement patterns. The progression of lower extremity biomechanics from the time of injury has not been described. OBJECTIVE:   To compare the 3-dimensional (3D) lower extremity kinematics and kinetics of walking among individuals with ACL deficiency (ACLD), individuals with ACLR, and healthy control participants from 3 to 64 months after ACLR...
August 11, 2017: Journal of Athletic Training
https://www.readbyqxmd.com/read/28799676/morphology-visualization-of-irregular-shape-bacteria-by-electron-holography-and-tomography
#20
Eduardo Ortega, Jesús Cantu-Valle, Germán Plascencia-Villa, Sandra Vergara, Fernando Mendoza-Santoyo, Alejandra Londono-Calderon, Ulises Santiago, Arturo Ponce
In the current work, irregular morphology of Staphylococcus aureus bacteria has been visualized by phase retrieval employing off-axis electron holography (EH) and 3D reconstruction electron tomography using high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM). Bacteria interacting with gold nanoparticles (AuNP) acquired a shrunken or irregular shape due to air dehydration processing. STEM imaging shows the attachment of AuNP on the surface of cells and suggests an irregular 3D morphology of the specimen...
August 11, 2017: Microscopy Research and Technique
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