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https://www.readbyqxmd.com/read/28817827/role-of-next-generation-sequencing-as-a-diagnostic-tool-for-the-evaluation-of-bone-and-soft-tissue-tumors
#1
Kinga Szurian, Karl Kashofer, Bernadette Liegl-Atzwanger
Bone and soft-tissue tumors are in general rare. Diagnosing these tumors is challenging based on the significant number of different tumor entities, the rareness of these tumors, and the considerable morphological heterogeneity which can be found within a single tumor entity. Considering that more than half of the described soft-tissue tumors and approximately 25% of the bone tumors harbor recurrent genetic alterations, the use of auxiliary molecular examinations should be strongly considered. Molecular analyses are important to confirm the diagnosis, to guide treatment, to provide information about prognosis, and to allow patient recruitment for basket trials based on the molecular signature of a tumor...
August 18, 2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28817802/molecular-memory-of-morphologies-by-septins-during-neuron-generation-allows-early-polarity-inheritance
#2
Leila Boubakar, Julien Falk, Hugo Ducuing, Karine Thoinet, Florie Reynaud, Edmund Derrington, Valérie Castellani
Transmission of polarity established early during cell lineage history is emerging as a key process guiding cell differentiation. Highly polarized neurons provide a fascinating model to study inheritance of polarity over cell generations and across morphological transitions. Neural crest cells (NCCs) migrate to the dorsal root ganglia to generate neurons directly or after cell divisions in situ. Using live imaging of vertebrate embryo slices, we found that bipolar NCC progenitors lose their polarity, retracting their processes to round for division, but generate neurons with bipolar morphology by emitting processes from the same locations as the progenitor...
August 16, 2017: Neuron
https://www.readbyqxmd.com/read/28817696/phenotypic-and-molecular-characterization-of-sweet-sorghum-accessions-for-bioenergy-production
#3
Michele Jorge da Silva, Maria Marta Pastina, Vander Fillipe de Souza, Robert Eugene Schaffert, Pedro Crescêncio Souza Carneiro, Roberto Willians Noda, José Eustáquio de Souza Carneiro, Cynthia Maria Borges Damasceno, Rafael Augusto da Costa Parrella
Sweet sorghum [Sorghum bicolor (L.) Moench] is a type of cultivated sorghum characterized by the accumulation of high levels of sugar in the stems and high biomass accumulation, making this crop an important feedstock for bioenergy production. Sweet sorghum breeding programs that focus on bioenergy have two main goals: to improve quantity and quality of sugars in the juicy stem and to increase fresh biomass productivity. Genetic diversity studies are very important for the success of a breeding program, especially in the early stages, where understanding the genetic relationship between accessions is essential to identify superior parents for the development of improved breeding lines...
2017: PloS One
https://www.readbyqxmd.com/read/28817687/morphologic-phenotypic-and-transcriptomic-characterization-of-classically-and-alternatively-activated-canine-blood-derived-macrophages-in-vitro
#4
Franziska Heinrich, Annika Lehmbecker, Barbara B Raddatz, Kristel Kegler, Andrea Tipold, Veronika M Stein, Arno Kalkuhl, Ulrich Deschl, Wolfgang Baumgärtner, Reiner Ulrich, Ingo Spitzbarth
Macrophages are a heterogeneous cell population playing a pivotal role in tissue homeostasis and inflammation, and their phenotype strongly depends on the micromilieu. Despite its increasing importance as a translational animal model for human diseases, there is a considerable gap of knowledge with respect to macrophage polarization in dogs. The present study comprehensively investigated the morphologic, phenotypic, and transcriptomic characteristics of unstimulated (M0), M1- (GM-CSF, LPS, IFNγ-stimulated) and M2- (M-CSF, IL-4-stimulated)-polarized canine blood-derived macrophages in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28817644/cheek-tooth-morphology-and-ancient-mitochondrial-dna-of-late-pleistocene-horses-from-the-western-interior-of-north-america-implications-for-the-taxonomy-of-north-american-late-pleistocene-equus
#5
Christina I Barrón-Ortiz, Antonia T Rodrigues, Jessica M Theodor, Brian P Kooyman, Dongya Y Yang, Camilla F Speller
Horses were a dominant component of North American Pleistocene land mammal communities and their remains are well represented in the fossil record. Despite the abundant material available for study, there is still considerable disagreement over the number of species of Equus that inhabited the different regions of the continent and on their taxonomic nomenclature. In this study, we investigated cheek tooth morphology and ancient mtDNA of late Pleistocene Equus specimens from the Western Interior of North America, with the objective of clarifying the species that lived in this region prior to the end-Pleistocene extinction...
2017: PloS One
https://www.readbyqxmd.com/read/28817640/evaluation-of-multilocus-marker-efficacy-for-delineating-mangrove-species-of-west-coast-india
#6
Ankush Ashok Saddhe, Rahul Arvind Jamdade, Kundan Kumar
The plant DNA barcoding is a complex and requires more than one marker(s) as compared to animal barcoding. Mangroves are diverse estuarine ecosystem prevalent in the tropical and subtropical zone, but anthropogenic activity turned them into the vulnerable ecosystem. There is a need to build a molecular reference library of mangrove plant species based on molecular barcode marker along with morphological characteristics. In this study, we tested the core plant barcode (rbcL and matK) and four promising complementary barcodes (ITS2, psbK-psbI, rpoC1 and atpF-atpH) in 14 mangroves species belonging to 5 families from West Coast India...
2017: PloS One
https://www.readbyqxmd.com/read/28817607/computer-aided-position-planning-of-miniplates-to-treat-facial-bone-defects
#7
Jan Egger, Jürgen Wallner, Markus Gall, Xiaojun Chen, Katja Schwenzer-Zimmerer, Knut Reinbacher, Dieter Schmalstieg
In this contribution, a software system for computer-aided position planning of miniplates to treat facial bone defects is proposed. The intra-operatively used bone plates have to be passively adapted on the underlying bone contours for adequate bone fragment stabilization. However, this procedure can lead to frequent intra-operatively performed material readjustments especially in complex surgical cases. Our approach is able to fit a selection of common implant models on the surgeon's desired position in a 3D computer model...
2017: PloS One
https://www.readbyqxmd.com/read/28817564/cell-cycle-related-kinase-ccrk-regulates-ciliogenesis-and-hedgehog-signaling-in-mice
#8
Ashley Snouffer, Desmond Brown, Hankyu Lee, Jonathon Walsh, Floria Lupu, Ryan Norman, Karl Lechtreck, Hyuk Wan Ko, Jonathan Eggenschwiler
The Hedgehog (Hh) signaling pathway plays a key role in cell fate specification, proliferation, and survival during mammalian development. Cells require a small organelle, the primary cilium, to respond properly to Hh signals and the key regulators of Hh signal transduction exhibit dynamic localization to this organelle when the pathway is activated. Here, we investigate the role of Cell Cycle Related kinase (CCRK) in regulation of cilium-dependent Hh signaling in the mouse. Mice mutant for Ccrk exhibit a variety of developmental defects indicative of inappropriate regulation of this pathway...
August 17, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28817404/primary-renal-sarcomas-with-bcor-ccnb3-gene-fusion-a-report-of-2-cases-showing-histologic-overlap-with-clear-cell-sarcoma-of-kidney-suggesting-further-link-between-bcor-related-sarcomas-of-the-kidney-and-soft-tissues
#9
Pedram Argani, Yu-Chien Kao, Lei Zhang, Carlos Bacchi, Andres Matoso, Rita Alaggio, Jonathan I Epstein, Cristina R Antonescu
We report 2 primary renal sarcomas demonstrating BCOR-CCNB3 gene fusions that have recently been identified in undifferentiated round cell sarcomas of bone and soft tissue. These neoplasms occurred in male children aged 11 and 12 years, and both were cystic as a result of entrapment and dilatation of native renal tubules. Both cases were composed of variably cellular bland spindle cells with fine chromatin set in myxoid stroma and separated by a branching capillary vasculature. Both neoplasms demonstrated immunoreactivity for BCOR, cyclin D1, TLE1, and SATB2 in the spindle neoplastic cells and negativity in the prominent capillary vasculature...
August 16, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28817355/total-training-load-may-explain-similar-strength-gains-and-muscle-hypertrophy-seen-in-aged-rats-submitted-to-resistance-training-and-anabolic-steroids
#10
Walter Krause Neto, Wellington de Assis Silva, Adriano Polican Ciena, Danilo Bocalini, Ricardo Aparecido Baptista Nucci, Carlos Alberto Anaruma, Eliane Florencio Gama
This study aimed to quantify training parameters and analyze the morphological response of aged muscles submitted to resistance training and anabolic steroids. Aged Wistar rats were divided into groups: C - initial control; CF - final control; CAS - control with anabolic steroid, RT - resistance training, and RTA - resistance training with anabolic steroid. Maximum carried load, absolute and relative loads increased significantly in RT and RTA. RTA demonstrated greater relative load than RT. Average total volume, total climbing volume, relative total volume, relative total climbing volume, and mean climbing volume were similar between groups RT and RTA...
August 17, 2017: Aging Male: the Official Journal of the International Society for the Study of the Aging Male
https://www.readbyqxmd.com/read/28817279/soft-chemical-fabrication-of-iron-based-thin-film-electrocatalyst-for-water-oxidation-under-neutral-ph-and-structure-activity-tuning-by-cerium-incorporation
#11
Jony Saha, T P Radhakrishnan
Design of electrocatalysts for the fundamentally important oxygen evolution reaction can be greatly aided by systematic structure-activity tuning via composition variation. We have explored the iron-cerium system as they are the most abundant transition and rare earth metals, and also due to the mutualistic impact of their size and electronic attributes that can induce critical changes in the structure and electrochemical activity. Submicrometer thick films of a series of Fe(III)-Ce(III) phosphate(oxyhydroxide) (FeCePH) are fabricated using a soft chemical strategy involving surfactant-aided assembly, spin-coating, and mild thermal annealing...
August 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28817263/responses-of-periphyton-to-fe2o3-nanoparticles-a-physiological-and-ecological-basis-for-defending-nanotoxicity
#12
Jun Tang, Ningyuan Zhu, Yan Zhu, Junzhuo Liu, Chenxi Wu, Philip G Kerr, Yonghong Wu, Kwan Sing Paul Kwan-Sing Lam
The toxic effects of nanoparticles on individual organisms have been widely investigated, while few studies have investigated the effects of nanoparticles on ubiquitous multi-community microbial aggregates. Here, periphyton as a model of microbial aggregates, was employed to investigate the responses of microbial aggregates exposed continuously to Fe2O3 nanoparticles (5.0 mg L-1) for 30 days. The Fe2O3 nanoparticles enter the microbial cells of the periphyton and disperse in the cytoplasm. The exposure to Fe2O3 nanoparticles results in the chlorophyll (a, b and c) contents of periphyton increasing and the total antioxidant capacity decreasing...
August 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28817218/bmi-nephroangiosclerosis-and-glomerulonephritis-is-there-any-meeting-point
#13
Antonietta Gigante, Konstantinos Giannakakis, Francesca Di Mario, Biagio Barbano, Edoardo Rosato, Riccardo Pofi, Marcello Di Paolo, Anna Rachele Rocca, Rosario Cianci
BACKGROUND: Overweight has been related to renal arteriolosclerosis and is able to modify intrarenal hemodynamics. Increasing evidences suggest an association between weight excess and primary glomerulonephritis (GN). The aim of this study was to evaluate the relationship between nutritional status and intrarenal arterial stiffness in primary GN associated to arteriolosclerosis. We have considered the glomerular diameter (GD) as morphological parameter in overweight and obese patients...
August 17, 2017: Nephrology
https://www.readbyqxmd.com/read/28817153/a-comparative-study-on-the-cardiac-morphology-and-vertical-jump-height-of-adolescent-black-south-african-male-and-female-amateur-competitive-footballers
#14
Philippe Jean-Luc Gradidge, Demitri Constantinou
OBJECTIVE: The aim of this comparative study was to determine the gender differences in cardiac morphology and performance in adolescent black South African footballers. METHODS: Anthropometry, electrocardiography and echocardiography data were measured in 167 (85 males and 82 females) adolescent black South African footballers (mean age: 14.8 ± 1.3 years). Vertical jump height was used as a performance measure of explosive lower-limb power. RESULTS: The males had less body fat compared with the females (12...
August 15, 2017: Cardiovascular Journal of Africa
https://www.readbyqxmd.com/read/28817142/morphologic-patterns-of-the-atrophic-posterior-maxilla-and-clinical-implications-for-bone-regenerative-therapy
#15
Alberto Monje, Istvan A Urban, Richard J Miron, Jordi Caballe-Serrano, Daniel Buser, Hom-Lay Wang
Regenerative therapies are commonly needed prior to implant placement in the posterior maxilla following tooth extraction. The aim of this study was to investigate the anatomical features of the atrophic posterior maxilla that might affect surgical approach selection and technique and risk of intra- and postoperative complications. Cone beam computed tomography files were screened to identify pristine atrophic maxillary ridges in need of bone augmentation (ridge height < 10 mm). Ridge height and width and lateral wall thickness (LWT) were measured at different levels, and palatonasal recess and lateral wall morphology were calculated...
September 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/28817100/modulatory-effects-of-exogenously-applied-polyamines-on-postharvest-physiology-antioxidant-system-and-shelf-life-of-fruits-a-review
#16
REVIEW
Sunil Sharma, Sunil Pareek, Narashans Alok Sagar, Daniel Valero, Maria Serrano
Polyamines (PAs) are natural compounds involved in many growth and developmental processes in plants, and, specifically in fruits, play a vital role regulating its development, ripening and senescence processes. Putrescine (PUT), spermine (SPE), and spermidine (SPD) are prominent PAs applied exogenously to extend shelf life of fruits. They also originate endogenously during developmental phases of horticultural crops and simultaneously affect the quality attributes and shelf life. Their anti-ethylene nature is being exploited to enhance the shelf life when exogenously applied on fruits...
August 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28817091/production-of-synthetic-phosphoanhydrite-and-its-use-as-a-binder-in-self-leveling-underlayments-slu
#17
Cecília Ogliari Schaefer, Malik Cheriaf, Janaíde Cavalcante Rocha
An experimental study was conducted to investigate the potential use of phosphogypsum (PG) to produce self-leveling underlayments. The study was designed in two stages. Initially a phosphoanhydrite (PA) was produced by heating phosphogypsum at temperatures of 350 °C, 450 °C, 550 °C, and 650 °C. Two periods of heating were applied (2 and 4 h). The formation of anhydrite was determined by thermogravimetric analysis (DTA-TG) and confirmed by X-ray diffraction (XRD). The results show that anhydrite II was obtained at temperatures above 450 °C, and at higher calcination temperatures the PA solubility was lower...
August 17, 2017: Materials
https://www.readbyqxmd.com/read/28817086/non-destructive-analysis-of-the-internal-anatomical-structures-of-mosquito-specimens-using-optical-coherence-tomography
#18
Naresh Kumar Ravichandran, Ruchire Eranga Wijesinghe, Seung-Yeol Lee, Kwang Shik Choi, Mansik Jeon, Hee-Young Jung, Jeehyun Kim
The study of mosquitoes and analysis of their behavior are of crucial importance in the on-going efforts to control the alarming increase in mosquito-borne diseases. Furthermore, a non-destructive and real-time imaging technique to study the anatomical features of mosquito specimens can greatly aid the study of mosquitoes. In this study, we demonstrate the three-dimensional imaging capabilities of optical coherence tomography (OCT) for structural analysis of Anopheles sinensis mosquitoes. The anatomical features of An...
August 17, 2017: Sensors
https://www.readbyqxmd.com/read/28816975/incidence-and-risk-factors-of-posterior-cage-migration-following-decompression-and-instrumented-fusion-for-degenerative-lumbar-disorders
#19
Hua Li, Hui Wang, Yanbo Zhu, Wenyuan Ding, Qian Wang
The aim of this study was to explore the incidence and risk factors for posterior cage migration (PCM) following decompression and instrumented fusion for degenerative lumbar disorders, and hope to provide references in decision making and surgical planning for spine surgeons.By retrieving the medical records from January 2011 to December 2015, 286 patients were retrospectively reviewed. According to the occurrence of PCM, patients were divided into 2 groups: PCM group and non-PCM (N-PCM). To investigate risk values for PCM, 3 categorized factors were analyzed statistically: patient characteristics: age, sex, body mass index, bone mineral density, duration of disease, diagnosis, comorbidity, smoke; surgical variables: surgery time, blood loss, surgical strategy, cage morphology, cage size, surgical segment, fusion number, source of bone graft, surgeon experience; radiographic parameters: preoperative lumbar lordosis, correction of lumbar lordosis, preoperative lumbar mobility, preoperative intervertebral height, change of intervertebral height, Modic changes, paraspinal muscle degeneration...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28816897/surgical-correction-of-cam-deformity-in-association-with-femoroacetabular-impingement-and-its-impact-on-the-degenerative-process-within-the-hip-joint
#20
Paul E Beaulé, Andrew D Speirs, Helen Anwander, Gerd Melkus, Kawan Rakhra, Hanspeter Frei, Mario Lamontagne
BACKGROUND: Cam morphology in association with femoroacetabular impingement (FAI) is a recognized cause of hip pain and cartilage damage and proposed as a leading cause of arthritis. The purpose of this study was to analyze the functional and biomechanical effects of the surgical correction of the cam deformity on the degenerative process associated with FAI. METHODS: Ten male patients with a mean age of 34.3 years (range, 23.1 to 46.5 years) and a mean body mass index (and standard deviation) of 26...
August 16, 2017: Journal of Bone and Joint Surgery. American Volume
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