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Protein lipid interaction

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https://www.readbyqxmd.com/read/29223532/new-insights-into-the-influence-of-monofluorination-on-dimyristoylphosphatidylcholine-membrane-properties-a-solid-state-nmr-study
#1
Marie-Claude Gagnon, Erik Strandberg, Anne S Ulrich, Jean-François Paquin, Michèle Auger
Solid-state 19F NMR spectroscopy is a method of choice to study the interactions between lipid membranes and other molecules such as peptides, proteins or drugs. Numerous fluorine-labeled NMR probes have been developed over the last few years, especially fluorine-labeled peptides. In order to develop a new kind of NMR reporter molecule and a complementary approach to fluorine-labeling of peptides, we synthesized six monofluorinated derivatives of the lipid dimyristoylphosphatidylcholine (F-DMPC), with the fluorine atom located along the acyl chain linked to the central glycerol position...
December 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29223087/oxidative-stress-in-birds-along-a-nox-and-urbanisation-gradient-an-interspecific-approach
#2
Pablo Salmón, Emilie Stroh, Amparo Herrera-Dueñas, Maria von Post, Caroline Isaksson
Urbanisation is regarded as one of the most threatening global issues for wildlife, however, measuring its impact is not always straight forward. Oxidative stress physiology has been suggested to be a useful biomarker of health and therefore, a potentially important indicator of the impact that urban environmental stressors, especially air pollution, can have on wildlife. For example, nitrogen oxides (NOx), released during incomplete combustion of fossil fuels, are highly potent pro-oxidants, thus predicted to affect either the protective antioxidants and/or cause oxidative damage to bio-molecules...
December 6, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29222644/membrane-shed-vesicles-from-the-parasite-trichomonas-vaginalis-characterization-and-their-association-with-cell-interaction
#3
Yesica R Nievas, Veronica M Coceres, Victor Midlej, Wanderley de Souza, Marlene Benchimol, Antonio Pereira-Neves, Ajay A Vashisht, James A Wohlschlegel, Patricia J Johnson, Natalia de Miguel
Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract, where it remains extracellular and adheres to epithelial cells. Infections range from asymptomatic to highly inflammatory, depending on the host and the parasite strain. Despite the serious consequences associated with trichomoniasis disease, little is known about parasite or host factors involved in attachment of the parasite-to-host epithelial cells. Here, we report the identification of microvesicle-like structures (MVs) released by T...
December 8, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29222176/ca2-releases-e-syt1-autoinhibition-to-couple-er-plasma-membrane-tethering-with-lipid-transport
#4
Xin Bian, Yasunori Saheki, Pietro De Camilli
The extended synaptotagmins (E-Syts) are endoplasmic reticulum (ER) proteins that bind the plasma membrane (PM) via C2 domains and transport lipids between them via SMP domains. E-Syt1 tethers and transports lipids in a Ca2+-dependent manner, but the role of Ca2+ in this regulation is unclear. Of the five C2 domains of E-Syt1, only C2A and C2C contain Ca2+-binding sites. Using liposome-based assays, we show that Ca2+ binding to C2C promotes E-Syt1-mediated membrane tethering by releasing an inhibition that prevents C2E from interacting with PI(4,5)P2-rich membranes, as previously suggested by studies in semi-permeabilized cells...
December 8, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29222162/dual-inhibition-of-pik3c3-and-fgfr-as-a-new-therapeutic-approach-to-treat-bladder-cancer
#5
Chun-Han Chen, C Changou, Tsung-Han Hsieh, Yu-Ching Lee, Cheng-Ying Chu, Kai-Cheng Hsu, Hao-Ching Wang, Yu-Chen Lin, Yan-Ni Lo, Yun-Ru Liu, Jing-Ping Liou, Yun Yen
PURPOSE: MPT0L145 has been developed as a FGFR inhibitor exhibiting significant anti-bladder cancer activity in vitro and in vivo via promoting autophagy-dependent cell death. Here, we aim to elucidate the underlying mechanisms. EXPERIMENTAL DESIGN: Autophagy flux, morphology and intracellular organelles were evaluated by western blotting, transmission electron microscope and fluorescence microscope. Molecular docking, surface plasmon resonance assay were performed to identify drug-protein interaction...
December 8, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29217594/glucose-induced-trophic-shift-in-an-endosymbiont-dinoflagellate-with-physiological-and-molecular-consequences
#6
Tingting Xiang, Robert E Jinkerson, Sophie Clowez, Cawa Tran, Cory J Krediet, Masayuki Onishi, Phillip A Cleves, John R Pringle, Arthur R Grossman
Interactions between the dinoflagellate endosymbiont Symbiodinium and its cnidarian hosts (e.g., corals, sea anemones) are the foundation of coral-reef ecosystems. Carbon flow between the partners is a hallmark of this mutualism, but the mechanisms governing this flow and its impact on symbiosis remain poorly understood. We showed previously that although Symbiodinium strain SSB01 can grow photoautotrophically, it can grow mixotrophically or heterotrophically when supplied with glucose, a metabolite normally transferred from the alga to its host...
December 7, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29216827/comparative-genomic-analysis-of-brevibacterium-strains-insights-into-key-genetic-determinants-involved-in-adaptation-to-the-cheese-habitat
#7
Nguyen-Phuong Pham, Séverine Layec, Eric Dugat-Bony, Marie Vidal, Françoise Irlinger, Christophe Monnet
BACKGROUND: Brevibacterium strains are widely used for the manufacturing of surface-ripened cheeses, contributing to the breakdown of lipids and proteins and producing volatile sulfur compounds and red-orange pigments. The objective of the present study was to perform comparative genomic analyses in order to better understand the mechanisms involved in their ability to grow on the cheese surface and the differences between the strains. RESULTS: The genomes of 23 Brevibacterium strains, including twelve strains isolated from cheeses, were compared for their gene repertoire involved in salt tolerance, iron acquisition, bacteriocin production and the ability to use the energy compounds present in cheeses...
December 7, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29213101/modulation-of-the-neisseria-gonorrhoeae-drug-efflux-conduit-mtre
#8
Giulia Tamburrino, Salomé Llabrés, Owen N Vickery, Samantha J Pitt, Ulrich Zachariae
Widespread antibiotic resistance, especially of Gram-negative bacteria, has become a severe concern for human health. Tripartite efflux pumps are one of the major contributors to resistance in Gram-negative pathogens, by efficiently expelling a broad spectrum of antibiotics from the organism. In Neisseria gonorrhoeae, one of the first bacteria for which pan-resistance has been reported, the most expressed efflux complex is MtrCDE. Here we present the electrophysiological characterisation of the outer membrane component MtrE and the membrane fusion protein MtrC, obtained by a combination of planar lipid bilayer recordings and in silico techniques...
December 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29211985/the-rna-binding-site-of-poliovirus-3c-protein-doubles-as-a-phosphoinositide-binding-domain
#9
Djoshkun Shengjuler, Yan Mei Chan, Simou Sun, Ibrahim M Moustafa, Zhen-Lu Li, David W Gohara, Matthias Buck, Paul S Cremer, David D Boehr, Craig E Cameron
Some viruses use phosphatidylinositol phosphate (PIP) to mark membranes used for genome replication or virion assembly. PIP-binding motifs of cellular proteins do not exist in viral proteins. Molecular-docking simulations revealed a putative site of PIP binding to poliovirus (PV) 3C protein that was validated using nuclear magnetic resonance spectroscopy. The PIP-binding site was located on a highly dynamic α helix, which also functions in RNA binding. Broad PIP-binding activity was observed in solution using a fluorescence polarization assay or in the context of a lipid bilayer using an on-chip, fluorescence assay...
December 5, 2017: Structure
https://www.readbyqxmd.com/read/29209467/biological-roles-and-potential-applications-of-immune-cell-derived-extracellular-vesicles
#10
REVIEW
Chuan Wen, Robert C Seeger, Muller Fabbri, Larry Wang, Alan S Wayne, Ambrose Y Jong
Extracellular vesicles (EVs) deliver bioactive macromolecules (i.e. proteins, lipids and nucleic acids) for intercellular communication in multicellular organisms. EVs are secreted by all cell types including immune cells. Immune cell-derived EVs modulate diverse aspects of the immune system to either enhance or suppress immune activities. The extensive effects of immune cell-derived EVs have become the focus of great interest for various nano-biomedical applications, ranging from the medical use of nanoplatform-based diagnostic agents to the development of therapeutic interventions as well as vaccine applications, and thus may be ideal for 'immune-theranostic'...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29209414/quantitative-in-vivo-phosphoproteomics-reveals-reversible-signaling-processes-during-nitrogen-starvation-and-recovery-in-the-biofuel-model-organism-chlamydomonas-reinhardtii
#11
Valentin Roustan, Shiva Bakhtiari, Pierre-Jean Roustan, Wolfram Weckwerth
Background: Nitrogen deprivation and replenishment induces massive changes at the physiological and molecular level in the green alga Chlamydomonas reinhardtii, including reversible starch and lipid accumulation. Stress signal perception and acclimation involves transient protein phosphorylation. This study aims to provide the first experimental phosphoprotein dataset for the adaptation of C. reinhardtii during nitrogen depletion and recovery growth phases and its impact on lipid accumulation...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29209002/the-orientation-and-stability-of-the-gpcr-arrestin-complex-in-a-lipid-bilayer
#12
Dali Wang, Hua Yu, Xiangdong Liu, Jianqiang Liu, Chen Song
G protein-coupled receptors (GPCRs) constitute a large family of membrane proteins that plays a key role in transmembrane signal transduction and draw wide attention since it was discovered. Arrestin is a small family of proteins which can bind to GPCRs, block G protein interactions and redirect signaling to G-protein-independent pathways. The detailed mechanism of how arrestin interacts with GPCR remains elusive. Here, we conducted molecular dynamics simulations with coarse-grained (CG) and all-atom (AA) models to study the complex structure formed by arrestin and rhodopsin, a prototypical GPCR, in a POPC bilayer...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29208950/x-ray-diffraction-reveals-the-intrinsic-difference-in-the-physical-properties-of-membrane-and-soluble-proteins
#13
Xavier Robert, Josiane Kassis-Sahyoun, Nicoletta Ceres, Juliette Martin, Michael R Sawaya, Randy J Read, Patrice Gouet, Pierre Falson, Vincent Chaptal
Membrane proteins are distinguished from soluble proteins by their insertion into biological membranes. This insertion is achieved via a noticeable arrangement of hydrophobic amino acids that are exposed at the surface of the protein, and renders the interaction with the aliphatic tails of lipids more energetically favorable. This important difference between these two categories of proteins is the source of the need for a specific handling of membrane proteins, which transpired in the creation of new tools for their recombinant expression, purification and even crystallization...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29207281/dark-chilling-and-subsequent-photo-activation-modulate-expression-and-induce-reversible-association-of-chloroplast-lipoxygenase-with-thylakoid-membrane-in-runner-bean-phaseolus-coccineus-l
#14
Radosław Mazur, Joanna Trzcinska-Danielewicz, Piotr Kozlowski, Łucja Kowalewska, Izabela Rumak, Brian J Shiell, Agnieszka Mostowska, Wojtek P Michalski, Maciej Garstka
Lipoxygenases (LOXs) are non-haem iron-containing dioxygenases that catalyse oxygenation of polyunsaturated fatty acids. This reaction is the first step in biosynthesis of oxylipins, which play important and diverse roles in stress response. In this study, we identified four LOX genes (PcLOXA, B, C, D) in chilling-sensitive runner bean (Phaseolus coccineus L.) plant and analyzed their expression patterns during long term dark-chilling (4 °C) stress and during day/night (21ºC/4 °C) temperature fluctuations...
November 26, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29205554/vapa-of-rhodococcus-equi-binds-phosphatidic-acid
#15
Lindsay M Wright, Emily M Carpinone, Terry L Bennett, Mary K Hondalus, Vincent J Starai
Rhodococcus equi is a multi-host, facultative intracellular bacterial pathogen that primarily causes pneumonia in foals less than six months in age and immunocompromised people. Previous studies determined that the major virulence determinant of R. equi is the surface bound virulence associated protein A (VapA). The presence of VapA inhibits the maturation of R. equi-containing phagosomes and promotes intracellular bacterial survival, as determined by the inability of vapA deletion mutants to replicate in host macrophages...
December 4, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29205175/anomalous-water-dynamics-at-surfaces-and-interfaces-synergistic-effects-of-confinement-and-surface-interactions
#16
Rajib Biswas, Biman Bagchi
In nature, water is often found in contact with surfaces that are extended on the scale of molecule size but small on a macroscopic scale. Examples include lipid bilayers and reverse micelles as well as biomolecules like proteins, DNA and zeolites, to name a few. While the presence of surfaces and interfaces interrupts the continuous hydrogen bond network of liquid water, confinement on a mesoscopic scale introduces new features. Even when extended on a molecular scale, natural and biological surfaces often have features (like charge, hydrophobicity) that vary on the scale of the molecular diameter of water...
December 5, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/29204879/reconstitution-of-membrane-tethering-mediated-by-rab-family-small-gtpases
#17
REVIEW
Joji Mima
Membrane tethering is one of the most critical steps to determine the spatiotemporal specificity of membrane trafficking, which is the process to selectively transport proteins, lipids, and other biological molecules to the appropriate locations in eukaryotic cells, such as subcellular organelles, the plasma membrane, and the extracellular space. Based on genetic, cell biological, biochemical, and structural studies, Rab-family small GTPases and a number of Rab-interacting proteins (termed Rab effectors), including coiled-coil tethering proteins and multisubunit tethering complexes, have been proposed to be key protein components for membrane tethering...
December 4, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/29204423/dual-coating-of-liposomes-as-encapsulating-matrix-of-antimicrobial-peptides-development-and-characterization
#18
Ahmed I Gomaa, Cynthia Martinent, Riadh Hammami, Ismail Fliss, Muriel Subirade
Antimicrobial peptides have been proposed as a potential biopreservatives in pharmaceutical research and agribusiness. However, many limitations hinder their utilization, such as their vulnerability to proteolytic digestion and their potential interaction with other food ingredients in complex food systems. One approach to overcome such problems is developing formulations entrapping and thereby protecting the antimicrobial peptides. Liposome encapsulation is a strategy that could be implemented to combine protection of the antimicrobial activity of the peptides from proteolytic enzymes and the controlled release of the encapsulated active ingredients...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29203416/quantitative-contributions-of-processes-by-which-polyanion-drugs-reduce-intracellular-bioavailability-and-transfection-efficiency-of-cationic-sirna-lipoplex
#19
Pharavee Jaiprasart, Bertrand Z Yeung, Ze Lu, M Guillaume Wientjes, Minjian Cui, Chien-Ming Hsieh, Sukyung Woo, Jessie L-S Au
RNA Interference (RNAi) is a potentially useful tool to correct the detrimental effects of faulty genes; several RNAi are undergoing clinical evaluation in various diseases. The present study identified the relative contributions of three mechanisms by which polyanion drugs reduced the gene silencing activity of Lipoplex, a complex of small interfering RNA (siRNA) and cationic liposomes. The study used a siRNA against the chemoresistance gene survivin and two model polyanion drugs (suramin, heparin). Products of Lipoplex destabilization were separated, identified, and/or quantified using ultrafiltration, gel electrophoresis, and RT-qPCR (quantitative reverse transcription polymerase chain reaction)...
December 1, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29202415/the-interplay-between-viperin-antiviral-activity-lipid-droplets-and-jun%C3%A3-n-mammarenavirus-multiplication
#20
José R Peña Cárcamo, María L Morell, Cecilia A Vázquez, Sezen Vatansever, Arunkumar S Upadhyay, Anna K Överby, Sandra M Cordo, Cybele C García
Junín arenavirus infections are associated with high levels of interferons in both severe and fatal cases. Upon Junín virus (JUNV) infection a cell signaling cascade initiates, that ultimately attempts to limit viral replication and prevent infection progression through the expression of host antiviral proteins. The interferon stimulated gene (ISG) viperin has drawn our attention as it has been highlighted as an important antiviral protein against several viral infections. The studies of the mechanistic actions of viperin have described important functional domains relating its antiviral and immune-modulating actions through cellular lipid structures...
December 1, 2017: Virology
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