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https://www.readbyqxmd.com/read/28223511/a-signal-sequence-suppressor-mutant-that-stabilizes-an-assembled-state-of-the-twin-arginine-translocase
#1
Qi Huang, Felicity Alcock, Holger Kneuper, Justin C Deme, Sarah E Rollauer, Susan M Lea, Ben C Berks, Tracy Palmer
The twin-arginine protein translocation (Tat) system mediates transport of folded proteins across the cytoplasmic membrane of bacteria and the thylakoid membrane of chloroplasts. The Tat system of Escherichia coli is made up of TatA, TatB, and TatC components. TatBC comprise the substrate receptor complex, and active Tat translocases are formed by the substrate-induced association of TatA oligomers with this receptor. Proteins are targeted to TatBC by signal peptides containing an essential pair of arginine residues...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28213463/membrane-transport-across-polarized-epithelia
#2
Maria Daniela Garcia-Castillo, Daniel J-F Chinnapen, Wayne I Lencer
Polarized epithelial cells line diverse surfaces throughout the body forming selective barriers between the external environment and the internal milieu. To cross these epithelial barriers, large solutes and other cargoes must undergo transcytosis, an endocytic pathway unique to polarized cell types, and significant for the development of cell polarity, uptake of viral and bacterial pathogens, transepithelial signaling, and immunoglobulin transport. Here, we review recent advances in our knowledge of the transcytotic pathway for proteins and lipids...
February 17, 2017: Cold Spring Harbor Perspectives in Biology
https://www.readbyqxmd.com/read/28205016/sensitivity-enhancement-for-membrane-proteins-reconstituted-in-parallel-and-perpendicular-oriented-bicelles-obtained-by-using-repetitive-cross-polarization-and-membrane-incorporated-free-radicals
#3
Sophie N Koroloff, Deanna M Tesch, Emmanuel O Awosanya, Alexander A Nevzorov
Multidimensional separated local-field and spin-exchange experiments employed by oriented-sample solid-state NMR are essential for structure determination and spectroscopic assignment of membrane proteins reconstituted in macroscopically aligned lipid bilayers. However, these experiments typically require a large number of scans in order to establish interspin correlations. Here we have shown that a combination of optimized repetitive cross polarization (REP-CP) and membrane-embedded free radicals allows one to enhance the signal-to-noise ratio by factors 2...
February 15, 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/28193719/brain-specific-ablation-of-efr3a-promotes-adult-hippocampal-neurogenesis-via-the-brain-derived-neurotrophic-factor-pathway
#4
Qi Qian, Qiuji Liu, Dongming Zhou, Hongyu Pan, Zhiwei Liu, Fangping He, Suying Ji, Dongpi Wang, Wangxiao Bao, Xinyi Liu, Zhaoling Liu, Heng Zhang, Xiaoqin Zhang, Ling Zhang, Mingkai Wang, Ying Xu, Fude Huang, Benyan Lao, Binggui Sun
Efr3 is a newly identified plasma membrane protein and plays an important role in the phosphoinositide metabolism on the plasma membrane. However, although it is highly expressed in the brain, the functional significance of Efr3 in the brain is not clear. In the present study, we generated Efr3a(f/f) mice and then crossed them with Nestin-Cre mice to delete Efr3a, one of the Efr3 isoforms, specifically in the brain. We found that brain-specific ablation of Efr3a promoted adult hippocampal neurogenesis by increasing survival and maturation of newborn neurons without affecting their dendritic tree morphology...
February 13, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28192162/transcriptional-regulation-of-rack1-and-modulation-of-its-expression-role-of-steroid-hormones-and-significance-in-health-and-aging
#5
Erica Buoso, Marilisa Galasso, Melania Maria Serafini, Melania Ronfani, Cristina Lanni, Emanuela Corsini, Marco Racchi
The Receptor for Activated C Kinase 1 (RACK1) is a scaffold protein for different kinases and membrane receptors. RACK1 can shuttle proteins to their sites of action, facilitate cross-talk among distinct signaling pathways or recruit other signaling proteins into the complexes. Therefore, it is a key mediator of various pathways and is involved in various biological events including development, immune response, brain activity and cancer. Because of its importance, it is of extreme significance to understand the transcriptional mechanisms governing its expression...
February 9, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28191706/g-protein-coupled-estrogen-receptor-and-estrogen-receptor-ligands-regulate-colonic-motility-and-visceral-pain
#6
M Zielińska, J Fichna, M Bashashati, S Habibi, A Sibaev, J-P Timmermans, M Storr
BACKGROUND: Diarrhea-predominant irritable bowel syndrome (IBS-D) is a functional gastrointestinal (GI) disorder, which occurs more frequently in women than men. The aim of our study was to determine the role of activation of classical estrogen receptors (ER) and novel membrane receptor, G protein-coupled estrogen receptor (GPER) in human and mouse tissue and to assess the possible cross talk between these receptors in the GI tract. METHODS: Immunohistochemistry was used to determine the expression of GPER in human and mouse intestines...
February 12, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28190758/intercellular-signalling-cross-talk-to-kill-to-heal-and-to-rejuvenate
#7
REVIEW
Egor Y Plotnikov, Denis N Silachev, Vasily A Popkov, Ljubava D Zorova, Irina B Pevzner, Savva D Zorov, Stanislovas S Jankauskas, Valentina A Babenko, Gennady T Sukhikh, Dmitry B Zorov
Intercellular cross-talk is a fundamental process for spreading cellular signals between neighbouring and distant cells to properly regulate their metabolism, to coordinate homeostasis, adaptation and survival as a functional tissue and organ. In this review, we take a close molecular view of the underpinning molecular mechanisms of such complex intercellular communications. There are several studied forms of cell-to-cell communications considered crucial for the maintenance of multicellular organisms. The most explored is paracrine signalling which is realised through the release of diffusible signalling factors (e...
January 23, 2017: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/28187200/ligand-induced-dynamics-of-heterotrimeric-g-protein-coupled-receptor-like-kinase-complexes
#8
Meral Tunc-Ozdemir, Alan M Jones
BACKGROUND: Arabidopsis, 7-transmembrane Regulator of G signaling protein 1 (AtRGS1) modulates canonical G protein signaling by promoting the inactive state of heterotrimeric G protein complex on the plasma membrane. It is known that plant leucine-rich repeat receptor-like kinases (LRR RLKs) phosphorylate AtRGS1 in vitro but little is known about the in vivo interaction, molecular dynamics, or the cellular consequences of this interaction. METHODS: Therefore, a subset of the known RLKs that phosphorylate AtRGS1 were selected for elucidation, namely, BAK1, BIR1, FLS2...
2017: PloS One
https://www.readbyqxmd.com/read/28186176/dual-immunomagnetic-nanobeads-based-lateral-flow-test-strip-for-simultaneous-quantitative-detection-of-carcinoembryonic-antigen-and-neuron-specific-enolase
#9
Wenting Lu, Kan Wang, Kun Xiao, Weijian Qin, Yafei Hou, Hao Xu, Xinyu Yan, Yanrong Chen, Daxiang Cui, Jinghua He
A novel immunomagnetic nanobeads -based lateral flow test strip was developed for the simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA), which are sensitive and specific in the clinical diagnosis of small cell lung cancer. Using this nanoscale method, high saturation magnetization, carboxyl-modified magnetic nanobeads were successfully synthesized. To obtain the immunomagnetic probes, a covalent bioconjugation of the magnetic nanobeads with the antibody of NSE and CEA was carried out...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28155651/incorporating-efficient-radial-basis-function-networks-and-significant-amino-acid-pairs-for-predicting-gtp-binding-sites-in-transport-proteins
#10
Nguyen-Quoc-Khanh Le, Yu-Yen Ou
BACKGROUND: Guanonine-protein (G-protein) is known as molecular switches inside cells, and is very important in signals transmission from outside to inside cell. Especially in transport protein, most of G-proteins play an important role in membrane trafficking; necessary for transferring proteins and other molecules to a variety of destinations outside and inside of the cell. The function of membrane trafficking is controlled by G-proteins via Guanosine triphosphate (GTP) binding sites...
December 22, 2016: BMC Bioinformatics
https://www.readbyqxmd.com/read/28153703/cross-talk-among-pmca-calcineurin-and-nfat-transcription-factors-in-control-of-calmodulin-gene-expression-in-differentiating-pc12-cells
#11
Tomasz Boczek, Malwina Lisek, Bozena Ferenc, Ludmila Zylinska
Brain aging is characterized by progressive loss of plasma membrane calcium pump (PMCA) and its activator - calmodulin (CaM), but the mechanism of this phenomenon remains unresolved. CaM encoded by three genes Calm1, Calm2, Calm3, works to translate Ca(2+) signal into changes in frequently opposite cellular activities. This unique function allows CaM to affect gene expression via stimulation of calcineurin (CaN) and its downstream target - nuclear factor of activated T-cells (NFAT) and to terminate Ca(2+) signal by stimulation of its extrusion...
January 30, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28141855/the-signal-peptide-like-segment-of-hpaxm-is-required-for-its-association-to-the-cell-wall-in-transgenic-tobacco-plants
#12
Le Li, Weiguo Miao, Wenbo Liu, Shujian Zhang
Harpins, encoded by hrp (hypersensitive response and pathogenicity) genes of Gram-negative plant pathogens, are elicitors of hypersensitive response (HR). HpaXm is a novel harpin-like protein described from cotton leaf blight bacteria, Xanthomonas citri subsp. malvacearum-a synonym of X. campestris pv. malvacearum (Smith 1901-1978). A putative signal peptide (1-MNSLNTQIGANSSFL-15) of hpaXm was predicted in the nitroxyl-terminal (N-terminal)by SignalP (SignalP 3.0 server). Here, we explored the function of the N-terminal leader peptide like segment of hpaXm using transgenic tobacco (Nicotiana tabacum cv...
2017: PloS One
https://www.readbyqxmd.com/read/28126662/on-chip-electrochemical-immunoassay-platform-for-specific-protein-biomarker-estimation-in-undiluted-serum-using-off-surface-membrane-matrix
#13
Sunil K Arya, Patthara Kongsuphol, Mi Kyoung Park
The manuscript presents a new biosensor platform using bioreceptors modified porous 2-dimensional (2D) membrane based off-surface matrix for on-chip electrochemical immunoassay. Antibody based bioreceptors modified 2D matrix of porous polycarbonate (PC) membrane with densely packed 20µm holes as off-surface matrix was incorporated in very close proximity of the sensor surface and integrated with fluidic system for reagent flow and incubation chamber. Covalent attachment of antibodies on 2D PC membrane based off-matrix was achieved using 4-fluoro-3-nitro-azidobenzene (FNAB) cross-linker...
May 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28119399/an-als-associated-mutant-sod1-rapidly-suppresses-kcnt1-slack-na-activated-k-channels-in-aplysia-neurons
#14
Yalan Zhang, Weiming Ni, Arthur L Horwich, Leonard K Kaczmarek
: Mutations that alter levels of Slack (KCNT1) Na(+)-activated K(+) current produce devastating effects on neuronal development and neuronal function. We now find that Slack currents are rapidly suppressed by oligomers of mutant human Cu/Zn superoxide dismutase 1 (SOD1), which are associated with motor neuron toxicity in an inherited form of amyotrophic lateral sclerosis (ALS). We recorded from bag cell neurons of Aplysia Californica, a model system to study neuronal excitability. We found that injection of fluorescent wild-type SOD1 (wt SOD1YFP) or monomeric mutant G85R SOD1YFP had no effect on net ionic currents measured under voltage clamp...
January 24, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28118086/a-plant-protein-signal-sequence-improved-humoral-immune-response-to-hpv-prophylactic-and-therapeutic-dna-vaccines
#15
Silvia Massa, Francesca Paolini, Gianfranca Curzio, Marcelo Nazario Cordeiro, Elena Illiano, Olivia Costantina Demurtas, Rosella Franconi, Aldo Venuti
Signal sequences (ss) play a critical role in the sorting of nascent secretory and membrane proteins. This function has been conserved from bacteria through eukaryotes, although ss appear diverse in length and amino acid composition. Sorting of proteins is also critical to instruct antigens for a proper immunological response. Thus, a plant ss was used to drive Human Papillomavirus (HPV) model antigens into the human secretory pathway: the HPV16 E7 oncoprotein, its chimera with the coat protein (CP) of the Potato Virus X (PVX), the first 200 amino acids of the HPV16 minor capsid protein L2 (known to harbour cross-reacting epitopes) and its chimera with E7 gene...
February 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28110141/synchrotron-fourier-transform-infrared-mapping-a-novel-approach-for-membrane-fouling-characterization
#16
Ming Xie, Wenhai Luo, Stephen R Gray
We described a synchrotron-based infrared (IR) microscopic method to characterize fouling layer induced by organic foulants and colloidal silica in membrane distillation (MD). This technique, utilizing the ultrahigh brightness of synchrotron infrared source, enables spectra with high signal-to-noise ratio that was obtained from micrometer-sized samples. Our results showed that synchrotron IR mapping was able to resolve the foulant spatial distribution in combined fouling in MD. Synchrotron IR mapping showed the spatial distribution of binary foulant (i...
January 11, 2017: Water Research
https://www.readbyqxmd.com/read/28107959/use-of-quantum-dot-beads-labeled-monoclonal-antibody-to-improve-the-sensitivity-of-a-quantitative-and-simultaneous-immunochromatographic-assay-for-neuron-specific-enolase-and-carcinoembryonic-antigen
#17
Kun Xiao, Kan Wang, Weijian Qin, Yafei Hou, Wenting Lu, Hao Xu, Yan Wo, Daxiang Cui
Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH-) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28073834/dynamic-and-coordinated-single-molecular-interactions-at-tm4sf5-enriched-microdomains-guide-invasive-behaviors-in-2-and-3-dimensional-environments
#18
Hye-Jin Kim, Sojung Kwon, Seo Hee Nam, Jae Woo Jung, Minkyung Kang, Jihye Ryu, Ji Eon Kim, Jin-Gyu Cheong, Chang Yun Cho, Somi Kim, Dae-Geun Song, Yong-Nyun Kim, Tai Young Kim, Min-Kyo Jung, Kyung-Min Lee, Chan-Gi Pack, Jung Weon Lee
Membrane proteins sense extracellular cues and transduce intracellular signaling to coordinate directionality and speed during cellular migration. They are often localized to specific regions, as with lipid rafts or tetraspanin-enriched microdomains; however, the dynamic interactions of tetraspanins with diverse receptors within tetraspanin-enriched microdomains on cellular surfaces remain largely unexplored. Here, we investigated effects of tetraspan(in) TM4SF5 (transmembrane 4 L six family member 5)-enriched microdomains (T5ERMs) on the directionality of cell migration...
January 10, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28068953/pregnant-human-peripheral-leukocyte-migration-during-several-late-pregnancy-clinical-conditions-a-cross-sectional-observational-study
#19
Jun Takeda, Xin Fang, David M Olson
BACKGROUND: Parturition at term and preterm is characterized by sterile inflammatory processes occurring in the absence of infection whereby peripheral leukocytes infiltrate gestational tissues in response to chemotactic signals. In response to a homing signal, recruited leukocytes undergo diapedesis and extravasate through capillaries, migrating into stromal tissue. There they interact with resident immune and stromal cells to produce a mixture of matrix metalloproteinases, prostaglandins and cytokines including interleukin-1β (IL-1β) and IL-6 that in turn transform the uterus from pregnancy to parturition...
January 10, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/28065260/biochemical-characterization-of-function-and-structure-of-rsep-an-escherichia-coli-s2p-protease
#20
Y Hizukuri, K Akiyama, Y Akiyama
Intramembrane-cleaving proteases (I-CLiPs) are a group of membrane-associated proteases with a unique feature: they are believed to cleave their substrate within the hydrophobic lipid bilayer, even though peptide bond hydrolysis requires a water molecule. Escherichia coli RseP, which belongs to the S2P zinc metalloprotease family of I-CLiPs, plays an essential role in activation of a cell envelope stress response through cleavage of anti-σ(E) protein RseA, a single-span transmembrane protein. A recent study showed that it also cleaves remnant signal peptides generated upon membrane translocation of secretory proteins...
2017: Methods in Enzymology
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