keyword
MENU ▼
Read by QxMD icon Read
search

Cross membrane signaling

keyword
https://www.readbyqxmd.com/read/28812765/rational-design-of-polymeric-core-shell-ratiometric-oxygen-sensing-nanostructures
#1
Aisling Byrne, Jaco Jacobs, Christopher S Burke, Aaron Martin, Andreas Heise, Tia E Keyes
A new approach for the fabrication of luminescent ratiometric sensing nanosensors is described using core-shell nanoparticles in which the probe and reference are spatially separated into the shell and core of the nanostructure respectively. The isolation of the reference in the core of the particle ensures a stable emission reference signal unaffected by the external environment. The core shell structure was prepared by engineering structurally well-defined Ru-conjugated block copolymers which acted as emulsifiers in the miniemulsion polymerisation of BODIPY loaded styrene nanoparticles...
August 16, 2017: Analyst
https://www.readbyqxmd.com/read/28799902/innate-immunity-to-intracellular-pathogens-balancing-microbial-elimination-and-inflammation
#2
REVIEW
Gabriel Mitchell, Ralph R Isberg
Recent excitement regarding immune clearance of intracellular microorganisms has focused on two systems that maintain cellular homeostasis. One system includes cellular autophagy components that mediate degradation of pathogens in membrane-bound compartments, in a process termed xenophagy. The second system is driven by interferon-regulated GTPases that promote rupture of pathogen-containing vacuoles and microbial degradation. In the case of xenophagy, pathogen sequestration and compartmentalization suppress inflammation...
August 9, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28794014/iron-affects-ire1-clustering-propensity-and-the-amplitude-of-endoplasmic-reticulum-stress-signaling
#3
Nir Cohen, Michal Breker, Anush Bakunts, Kristina Pesek, Ainara Chas, Josepmaria Argemí, Andrea Orsi, Lihi Gal, Silvia Chuartzman, Yoav Wigelman, Felix Jonas, Peter Walter, Robert Ernst, Tomás Aragón, Eelco van Anken, Maya Schuldiner
The unfolded protein response (UPR) allows cells to adjust secretory pathway capacity according to need. Ire1, the endoplasmic reticulum (ER) stress sensor and central activator of the UPR is conserved from the budding yeast Saccharomyces cerevisiae to humans. Under ER stress conditions, Ire1 clusters into foci that enable optimal UPR activation. To discover factors that affect Ire1 clustering, we performed a high-content screen using a whole-genome yeast mutant library expressing Ire1-mCherry. We imaged the strains following UPR induction and found 154 strains that displayed alterations in Ire1 clustering...
August 9, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28792496/stabilization-of-diastolic-calcium-signal-via-calcium-pump-regulation-of-complex-local-calcium-releases-and-transient-decay-in-a-computational-model-of-cardiac-pacemaker-cell-with-individual-release-channels
#4
Alexander V Maltsev, Victor A Maltsev, Michael D Stern
Intracellular Local Ca releases (LCRs) from sarcoplasmic reticulum (SR) regulate cardiac pacemaker cell function by activation of electrogenic Na/Ca exchanger (NCX) during diastole. Prior studies demonstrated the existence of powerful compensatory mechanisms of LCR regulation via a complex local cross-talk of Ca pump, release and NCX. One major obstacle to study these mechanisms is that LCR exhibit complex Ca release propagation patterns (including merges and separations) that have not been characterized. Here we developed new terminology, classification, and computer algorithms for automatic detection of numerically simulated LCRs and examined LCR regulation by SR Ca pumping rate (Pup) that provides a major contribution to fight-or-flight response...
August 8, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28790014/bmp2-expression-in-the-endocardial-lineage-is-required-for-av-endocardial-cushion-maturation-and-remodeling
#5
Jacob G Saxon, Daniel R Baer, Julie A Barton, Travis Hawkins, Bingruo Wu, Thomas C Trusk, Stephen E Harris, Bin Zhou, Yuji Mishina, Yukiko Sugi
Distal outgrowth, maturation and remodeling of the endocardial cushion mesenchyme in the atrioventricular (AV) canal are the essential morphogenetic events during four-chambered heart formation. Mesenchymalized AV endocardial cushions give rise to the AV valves and the membranous ventricular septum (VS). Failure of these processes results in several human congenital heart defects. Despite this clinical relevance, the mechanisms governing how mesenchymalized AV endocardial cushions mature and remodel into the membranous VS and AV valves have only begun to be elucidated...
August 5, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28755580/green-synthesized-nickel-nanoparticles-for-targeted-detection-and-killing-of-s-typhimurium
#6
Chitra Jeyaraj Pandian, Rameshthangam Palanivel, Usha Balasundaram
Simple and sensitive colorimetric immunosensor based on peroxidase mimetic activity and photothermal effect of nickel oxide nanoparticle (NiOGs) has been developed to detect and kill food borne pathogen Salmonella typhimurium. NiOGs showed superior peroxidase mimetic activity for oxidation of peroxidase substrate 3, 3', 5, 5'-tetramethylbenzidine (TMB). Oxidation of TMB by NiOGs followed Michaelis-Menten kinetics with Km and Vmax values of 0.25mM and 2.64×10(-8)M/s respectively. NiOGs was coated with citric acid (CA-NiOGs) followed by conjugation with antibody (anti-S...
July 21, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28752390/pdgfr%C3%AE-p2a-creer-t2-mice-a-genetic-tool-to-target-pericytes-in-angiogenesis
#7
Henar Cuervo, Brianna Pereira, Taliha Nadeem, Mika Lin, Frances Lee, Jan Kitajewski, Chyuan-Sheng Lin
Pericytes are essential mural cells distinguished by their association with small caliber blood vessels and the presence of a basement membrane shared with endothelial cells. Pericyte interaction with the endothelium plays an important role in angiogenesis; however, very few tools are currently available that allow for the targeting of pericytes in mouse models, limiting our ability to understand their biology. We have generated a novel mouse line expressing tamoxifen-inducible Cre-recombinase under the control of the platelet-derived growth factor receptor β promoter: PDGFRβ-P2A-CreER (T2) ...
July 27, 2017: Angiogenesis
https://www.readbyqxmd.com/read/28751549/cross-talk-of-brassinosteroid-signaling-in-controlling-growth-and-stress-responses
#8
REVIEW
Trevor Nolan, Jiani Chen, Yanhai Yin
Plants are faced with a barrage of stresses in their environment and must constantly balance their growth and survival. As such, plants have evolved complex control systems that perceive and respond to external and internal stimuli in order to optimize these responses, many of which are mediated by signaling molecules such as phytohormones. One such class of molecules called Brassinosteroids (BRs) are an important group of plant steroid hormones involved in numerous aspects of plant life including growth, development and response to various stresses...
July 27, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28747871/age-and-glaucoma-related-characteristics-in-retinal-nerve-fiber-layer-and-choroid-localized-morphometrics-and-visualization-using-functional-shapes-registration
#9
Sieun Lee, Morgan L Heisler, Karteek Popuri, Nicolas Charon, Benjamin Charlier, Alain Trouvé, Paul J Mackenzie, Marinko V Sarunic, Mirza Faisal Beg
Optical coherence tomography provides high-resolution 3D imaging of the posterior segment of the eye. However, quantitative morphological analysis, particularly relevant in retinal degenerative diseases such as glaucoma, has been confined to simple sectorization and averaging with limited spatial sensitivity for detection of clinical markers. In this paper, we present point-wise analysis and visualization of the retinal nerve fiber layer and choroid from cross-sectional data using functional shapes (fshape) registration...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28739882/zebrafish-b-cell-development-without-a-pre-b-cell-stage-revealed-by-cd79-fluorescence-reporter-transgenes
#10
Xingjun Liu, Yue-Sheng Li, Susan A Shinton, Jennifer Rhodes, Lingjuan Tang, Hui Feng, Cicely A Jette, A Thomas Look, Kyoko Hayakawa, Richard R Hardy
CD79a and CD79b proteins associate with Ig receptors as integral signaling components of the B cell Ag receptor complex. To study B cell development in zebrafish, we isolated orthologs of these genes and performed in situ hybridization, finding that their expression colocalized with IgH-μ in the kidney, which is the site of B cell development. CD79 transgenic lines were made by linking the promoter and upstream regulatory segments of CD79a and CD79b to enhanced GFP to identify B cells, as demonstrated by PCR analysis of IgH-μ expression in sorted cells...
July 24, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28729673/distinct-functional-relevance-of-dynamic-gtpase-cysteine-methylation-in-fission-yeast
#11
Alejandro Franco, Teresa Soto, Rebeca Martín-García, Marisa Madrid, Beatriz Vázquez-Marín, Jero Vicente-Soler, Pedro M Coll, Mariano Gacto, Pilar Pérez, José Cansado
The final step in post-translational processing of Ras and Rho GTPases involves methylation of the prenylated cysteine residue by an isoprenylcysteine-O-carboxyl methyltransferase (ICMT). ICMT activity is essential for cell growth and development in higher eukaryotes, and inhibition of GTPase methylation has become an attractive target in cancer therapy to inactivate prenylated oncoproteins. However, the specificity and dynamics of the GTPase methylation process remain to be fully clarified. Notably, cells lacking Mam4, the ICMT ortholog in the fission yeast Schizosaccharomyces pombe, are viable...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28727410/exosomes-mediate-epithelium-mesenchyme-crosstalk-in-organ-development
#12
Nan Jiang, Lusai Xiang, Ling He, Guodong Yang, Jinxuan Zheng, Chenglin Wang, Yimei Zhang, Sainan Wang, Yue Zhou, Tzong-Jen Sheu, Jiaqian Wu, Kenian Chen, Paulo G Coelho, Nicky M Tovar, Shin Hye Kim, Mo Chen, Yan-Heng Zhou, Jeremy J Mao
Organ development requires complex signaling by cells in different tissues. Epithelium and mesenchyme interactions are crucial for the development of skin, hair follicles, kidney, lungs, prostate, major glands, and teeth. Despite myriad literature on cell-cell interactions and ligand-receptor binding, the roles of extracellular vesicles in epithelium-mesenchyme interactions during organogenesis are poorly understood. Here, we discovered that ∼100 nm exosomes were secreted by the epithelium and mesenchyme of a developing tooth organ and diffused through the basement membrane...
July 27, 2017: ACS Nano
https://www.readbyqxmd.com/read/28717930/understanding-the-phenotypic-similarities-between-ifap-and-olmsted-syndrome-from-a-molecular-perspective-the-interaction-of-mbtps2-and-trpv3
#13
Georges Nemer, Rémi Safi, Firas Kreidieh, Julnar Usta, Christina Bergqvist, Farah Ballout, Waed Btadini, Nour Hamzeh, Ossama Abbas, Abdul Ghani Kibbi, Yutaka Shimomura, Mazen Kurban
Ichthyosis Follicularis, Atrichia, and Photophobia (IFAP) is a severe rare genetic disorder caused by mutations in the gene encoding the Membrane-Bound Transcription Factor Peptidase, Site 2 (MBTPS2). Olmsted syndrome is another rare genetic disease with overlapping clinical features caused by mutations in the gene encoding the Transient Receptor Potential Cation Channel, subfamily V (TRPV3). Mutations in MBTPS2 have been recently reported in Olmsted syndrome, underscoring the overlap and the confusion in separating Olmsted from IFAP syndrome...
July 17, 2017: Archives of Dermatological Research
https://www.readbyqxmd.com/read/28706161/the-molecular-mechanisms-of-chemoresistance-in-cancers
#14
REVIEW
Hua-Chuan Zheng
Overcoming intrinsic and acquired drug resistance is a major challenge in treating cancer patients because chemoresistance causes recurrence, cancer dissemination and death. This review summarizes numerous molecular aspects of multi-resistance, including transporter pumps, oncogenes (EGFR, PI3K/Akt, Erk and NF-κB), tumor suppressor gene (p53), mitochondrial alteration, DNA repair, autophagy, epithelial-mesenchymal transition (EMT), cancer stemness, and exosome. The chemoresistance-related proteins are localized to extracellular ligand, membrane receptor, cytosolic signal messenger, and nuclear transcription factors for various events, including proliferation, apoptosis, EMT, autophagy and exosome...
July 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28685119/surface-enhanced-raman-spectroscopy-of-cell-lysates-mixed-with-silver-nanoparticles-for-tumor-classification
#15
Mohamed Hassoun, Iwan W Schie, Tatiana Tolstik, Sarmiza E Stanca, Christoph Krafft, Juergen Popp
The throughput of spontaneous Raman spectroscopy for cell identification applications is limited to the range of one cell per second because of the relatively low sensitivity. Surface-enhanced Raman scattering (SERS) is a widespread way to amplify the intensity of Raman signals by several orders of magnitude and, consequently, to improve the sensitivity and throughput. SERS protocols using immuno-functionalized nanoparticles turned out to be challenging for cell identification because they require complex preparation procedures...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28680058/pfcdpk1-mediated-signaling-in-erythrocytic-stages-of-plasmodium-falciparum
#16
Sudhir Kumar, Manish Kumar, Roseleen Ekka, Jeffrey D Dvorin, Aditya S Paul, Anil K Madugundu, Tim Gilberger, Harsha Gowda, Manoj T Duraisingh, T S Keshava Prasad, Pushkar Sharma
Calcium Dependent Protein Kinases are key effectors of calcium signaling in malaria parasite. PfCDPK1 is critical for asexual development of Plasmodium falciparum, but its precise function and substrates remain largely unknown. Using a conditional knockdown strategy, we here establish that this kinase is critical for the invasion of host erythrocytes. Furthermore, using a multidisciplinary approach involving comparative phosphoproteomics we gain insights into the underlying molecular mechanisms. We identify substrates of PfCDPK1, which includes proteins of Inner Membrane Complex and glideosome-actomyosin motor assembly...
July 5, 2017: Nature Communications
https://www.readbyqxmd.com/read/28679208/spatially-selective-dissection-of-signal-transduction-in-neurons-grown-on-netrin-1-printed-nanoarrays-via-segmented-fluorescence-fluctuation-analysis
#17
Angelica A Gopal, Sebastien G Ricoult, Stephanie N Harris, David Juncker, Timothy E Kennedy, Paul W Wiseman
Axonal growth cones extend during neural development in response to precise distributions of extracellular cues. Deleted in Colorectal Cancer (DCC), a receptor for the chemotropic guidance cue netrin-1, directs F-actin reorganization and is essential for mammalian neural development. To elucidate how the extracellular distribution of netrin-1 influences the distribution of DCC and F-actin within axonal growth cones, we patterned nanoarrays of substrate bound netrin-1 using lift off nanocontact printing. The distribution of DCC and F-actin in embryonic rat cortical neuron growth cones was then imaged using total internal reflection fluorescence (TIRF) microscopy...
July 5, 2017: ACS Nano
https://www.readbyqxmd.com/read/28676702/extracellular-matrix-promotes-clathrin-dependent-endocytosis-of-prolactin-and-stat5-activation-in-differentiating-mammary-epithelial-cells
#18
Rebecca E Bridgewater, Charles H Streuli, Patrick T Caswell
The hormone prolactin promotes lactational differentiation of mammary epithelial cells (MECs) via its cognate receptor and the downstream JAK2-STAT5a signalling pathway. In turn this regulates transcription of milk protein genes. Prolactin signalling depends on a cross-talk with basement membrane extracellular matrix (ECM) via β1 integrins which activate both ILK and Rac1 and are required for STAT5a activation and lactational differentiation. Endocytosis is an important regulator of signalling. It can both enhance and suppress cytokine signalling, although the role of endocytosis for prolactin signalling is not known...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28660664/engaging-ly-6a-sca-1-triggers-lipid-raft-dependent-and-independent-responses-in-cd4-t-cell-lines
#19
Melissa A Lang, Sultan A Jenkins, Phillip Balzano, Adeyinka Owoyele, Akshay Patel, Anil K Bamezai
INTRODUCTION: The lymphocyte antigen 6 (Ly-6) supergene family encodes proteins of 12-14 kda in molecular mass that are either secreted or anchored to the plasma membrane through a glycosyl-phosphatidylinisotol (GPI) lipid anchor at the carboxy-terminus. The lipidated GPI-anchor allows localization of Ly-6 proteins to the 10-100 nm cholesterol-rich nano-domains on the membrane, also known as lipid rafts. Ly-6A/Sca-1, a member of Ly-6 gene family is known to transduce signals despite the absence of transmembrane and cytoplasmic domains...
June 28, 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/28660582/single-step-capture-and-targeted-metabolomics-of-alkyl-quinolones-in-outer-membrane-vesicles-of-pseudomonas-aeruginosa
#20
Pallavi Lahiri, Dipankar Ghosh
Outer membrane vesicles (OMVs) are secreted by all Gram-ve pathogens. These nano-scale delivery vehicles contain discrete arrays of prokaryotic pathogenic determinants, including a family of low molecular weight (MW) lipidic quorum signaling alkyl-quinolones (AQs). These are synthesized from β-keto-fatty acids and function like primordial lipidic hormones, which regulate numerous pathogenic factors both inter-species and intra-species. Significantly, AQs can also directly exacerbate pathogenesis by cross-kingdom signaling with the host immune, metabolic, and other systems...
2017: Methods in Molecular Biology
keyword
keyword
54550
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"