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https://www.readbyqxmd.com/read/28628752/high-prevalence-of-resistance-to-fluoroquinolones-and-tetracycline-campylobacter-spp-isolated-from-poultry-in-poland
#1
Anna Woźniak-Biel, Gabriela Bugla-Płoskońska, Alicja Kielsznia, Kamila Korzekwa, Anna Tobiasz, Agnieszka Korzeniowska-Kowal, Alina Wieliczko
Campylobacter spp. is a major cause of foodborne diseases in humans, particularly when transmitted by the handling or consumption of undercooked poultry meat. Most Campylobacter infections are self-limiting, but antimicrobial treatment (e.g., fluoroquinolones and macrolides) is necessary in severe or prolonged cases. The indiscriminate use of these drugs, both in clinical medicine and animal production, has a major impact on public health. The aim of the present study was to identify Campylobacter strains, isolated from turkey and broilers, using both PCR and the matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) methods to reveal the accuracy of identification, as well to evaluate the antimicrobial and genetic resistance of the investigated strains...
June 19, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28618085/improved-localization-spectral-quality-and-repeatability-with-advanced-mrs-methodology-in-the-clinical-setting
#2
Dinesh K Deelchand, Kejal Kantarci, Gülin Öz
PURPOSE: To investigate the utility of an advanced magnetic resonance spectroscopy (MRS) protocol in the clinical setting, and to compare the localization accuracy, spectral quality, and quantification repeatability between this advanced and the conventional vendor-provided MRS protocol on a clinical 3T platform. METHODS: Proton spectra were measured from the posterior cingulate cortices in 30 healthy elderly subjects by clinical MR technologists using a vendor-provided (point resolved spectroscopy with advanced 3D gradient-echo B0 shimming) and an advanced (semi-LASER with FAST(EST)MAP shimming) protocol, in random order...
June 15, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28608445/tailoring-the-volatility-and-stability-of-oligopeptides
#3
J Schätti, U Sezer, S Pedalino, J P Cotter, M Arndt, M Mayor, V Köhler
Amino acids are essential building blocks of life and fluorinated derivatives have gained interest in chemistry and medicine. Modern mass spectrometry has enabled the study of oligo- and polypeptides as isolated entities in the gas phase, but predominantly as singly or even multiply charged species. While laser desorption of neutral peptides into adiabatically expanding supersonic noble gas jets is possible, UV-VIS spectroscopy, electric or magnetic deflectometry as well as quantum interferometry would profit from the possibility to prepare thermally slow molecular beams...
June 12, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28605359/production-of-metal-nanoparticles-by-pulsed-laser-ablation-in-liquids-a-tool-for-studying-the-antibacterial-properties-of-nanoparticles
#4
Matthew Ratti, Joseph J Naddeo, Julianne C Griepenburg, Sean M O'Malley, Daniel M Bubb, Eric A Klein
The emergence of multidrug-resistant bacteria is a global clinical concern leading some to speculate about our return to a "pre-antibiotics" era of medicine. In addition to efforts to identify novel small-molecule antimicrobial drugs, there has been great interest in the use of metal nanoparticles as coatings for medical devices, wound dressings, and consumer packaging, due to their antimicrobial properties. The wide variety of methods available for nanoparticle synthesis results in a broad spectrum of chemical and physical properties which can affect antibacterial efficacy...
June 2, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28605324/sulfation-of-the-extracellular-polysaccharide-produced-by-the-king-oyster-culinary-medicinal-mushroom-pleurotus-eryngii-agaricomycetes-and-its-antioxidant-properties-in-vitro
#5
Xuewei Jia, Chong Wang, Yanhong Bai, Jinwei Yu, Chunping Xu
Extracellular polysaccharide from Pleurotus eryngii was sulfated; the optimum reaction conditions used pyridine as the reaction solvent, a temperature of 80°C, and a reaction time of 90 minutes. The structure and antioxidant activity of the sulfated and original polysaccharides (designated as SF-1 and Fr-1, respectively) were investigated. The main functional groups of polysaccharides were determined by Fourier transform infrared, and the chain conformation was determined by size-exclusion chromatography with laser light scattering and viscometry...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28592299/real-versus-sham-proximal-biofield-therapy-in-the-treatment-of-warts-of-the-hands-and-feet-in-adults-study-protocol-for-a-randomized-controlled-trial-magnetik-study
#6
Cathy Gaillard, Laure Allain, Hélène Legros, Sylvie Brucato, Yohann Desgue, Christophe Rouillon, Laure Peyro-Saint-Paul, Anne Dompmartin
BACKGROUND: Despite the lack of scientific studies on biofield therapies, they are widely acclaimed by patients. The mechanisms of action are not explained by current allopathic medical approaches. Warts are common and contagious viral lesions that may be refractory to standard dermatologic treatments such as cryotherapy, laser therapy, and keratolytic ointments. Biofield therapies are efficient in various pathologies. Their ability to treat warts has never been demonstrated in a scientific study with a robust methodology...
June 7, 2017: Trials
https://www.readbyqxmd.com/read/28580277/expression-and-functional-assessment-of-candidate-type-2-diabetes-susceptibility-genes-identify-four-new-genes-contributing-to-human-insulin-secretion
#7
Fatou K Ndiaye, Ana Ortalli, Mickaël Canouil, Marlène Huyvaert, Clara Salazar-Cardozo, Cécile Lecoeur, Marie Verbanck, Valérie Pawlowski, Raphaël Boutry, Emmanuelle Durand, Iandry Rabearivelo, Olivier Sand, Lorella Marselli, Julie Kerr-Conte, Vikash Chandra, Raphaël Scharfmann, Odile Poulain-Godefroy, Piero Marchetti, François Pattou, Amar Abderrahmani, Philippe Froguel, Amélie Bonnefond
OBJECTIVES: Genome-wide association studies (GWAS) have identified >100 loci independently contributing to type 2 diabetes (T2D) risk. However, translational implications for precision medicine and for the development of novel treatments have been disappointing, due to poor knowledge of how these loci impact T2D pathophysiology. Here, we aimed to measure the expression of genes located nearby T2D associated signals and to assess their effect on insulin secretion from pancreatic beta cells...
June 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28575108/changes-in-the-leaf-proteome-profile-of-withania-somnifera-l-dunal-in-response-to-alternaria-alternata-infection
#8
Varinder Singh, Baldev Singh, Robin Joshi, Puneet Jaju, Pratap Kumar Pati
Withania somnifera is a high value medicinal plant which is used against large number of ailments. The medicinal properties of the plant attributes to a wide array of important secondary metabolites. The plant is predominantly infected with leaf spot pathogen Alternaria alternata, which leads to substantial biodeterioration of pharmaceutically important metabolites. To develop an effective strategy to combat this disease, proteomics based approach could be useful. Hence, in the present study, three different protein extraction methods tris-buffer based, phenol based and trichloroacetic acid-acetone (TCA-acetone) based method were comparatively evaluated for two-dimensional electrophoresis (2-DE) analysis of W...
2017: PloS One
https://www.readbyqxmd.com/read/28570005/news-announcements-american-society-for-laser-medicine-and-surgery-inc
#9
(no author information available yet)
No abstract text is available yet for this article.
July 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28565770/dose-dependent-effect-of-curcuma-longa-for-the-treatment-of-parkinson-s-disease
#10
Xiao-Wei Ma, Rui-You Guo
Curcuma longa is a plant that belongs to the ginger family, Zingiberaceae. It has been used in Siddha medicine for thousands of years in Asia. Parkinson's disease (PD) is a degenerative disorder of the central nervous system that affects the motor system of the brain. Death of dopamine-producing cells in the substantia nigra leads to PD. Exposure to salsolinol, which is an endogenous neurotoxin, has been associated with damage to dopamine-producing cells. The present study assessed the toxicity of salsolinol in SH-SY5Y human neuroblastoma cells and subsequently investigated the neuroprotective potential of C...
May 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28552305/matrix-assisted-laser-desorption-ionization-mass-spectrometric-imaging-for-the-rapid-segmental-analysis-of-methamphetamine-in-a-single-hair-using-umbelliferone-as-a-matrix
#11
Hang Wang, Ying Wang
Segmental hair analysis offers a longer period for retrospective drug detection than blood or urine. Hair is a keratinous fiber and is strongly hydrophobic. The embedding of drugs in hydrophobic hair at low concentrations makes it difficult for extraction and detection with matrix-assisted laser desorption/ionization (MALDI) coupled with mass spectrometric imaging (MSI). In this study, a single scalp hair was longitudinally cut with a cryostat section to a length of 4 mm and fixed onto a stainless steel MALDI plate...
July 4, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28549467/the-prediction-diagnosis-and-management-of-complications-in-monochorionic-twin-pregnancies-the-ommit-optimal-management-of-monochorionic-twins-study
#12
Fiona L Mackie, R Katie Morris, Mark D Kilby
BACKGROUND: Monochorionic twin pregnancies are at increased risk of complications due to sharing a single placenta and potentially developing unbalanced vascular anastomoses. Complications include twin-twin transfusion syndrome (TTTS) which affects 10-15% monochorionic twins, and if untreated has a 70-90% perinatal loss rate. We are currently unable to predict which twins will develop complications or to what severity. We have previously shown differences in angiogenic and placental growth factors in maternal blood in pregnancies complicated by TTTS compared to twin pregnancies not complicated by TTTS but matched for gestation...
May 26, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/28540540/molecular-mechanisms-of-bombyx-batryticatus-ethanol-extract-inducing-gastric-cancer-sgc-7901-cells-apoptosis
#13
Jin-Yi Wu, Almutamad Sheikho, He Ma, Tian-Ci Li, Ya-Qi Zhao, Ya-Lin Zhang, Dun Wang
Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of human gastric cancer cells SGC-7901. Cells treated with BBE and apoptosis was assessed by methyl thiazolyl tetrazolium (MTT) assay, morphological changes, DNA fragmentation and flow cytometry assays. The expression of Bcl-2, Bax and P21 were evaluated by western blot analysis and real time polymerase chain reaction. MTT assay showed that the cytotoxicity of BBE extract on SGC-7901 cells was correlated with treatment time and concentration...
May 24, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28537466/laser-medicine-and-surgery-as-a-single-comprehensive-and-multi-disciplinary-field
#14
Mario A Trelles
No abstract text is available yet for this article.
June 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28534676/applications-of-raman-spectroscopy-in-biopharmaceutical-manufacturing-a-short-review
#15
Kevin Buckley, Alan G Ryder
The production of active pharmaceutical ingredients (APIs) is currently undergoing its biggest transformation in a century. The changes are based on the rapid and dramatic introduction of protein- and macromolecule-based drugs (collectively known as biopharmaceuticals) and can be traced back to the huge investment in biomedical science (in particular in genomics and proteomics) that has been ongoing since the 1970s. Biopharmaceuticals (or biologics) are manufactured using biological-expression systems (such as mammalian, bacterial, insect cells, etc...
June 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28532933/clinical-outcomes-of-femtosecond-laser-assisted-cataract-surgery-versus-conventional-phacoemulsification-surgery-for-hard-nuclear-cataracts
#16
Xinyi Chen, Yinhui Yu, Xiaohui Song, Yanan Zhu, Wei Wang, Ke Yao
PURPOSE: To compare the outcomes between femtosecond laser-assisted cataract surgery (femtosecond group) and conventional phacoemulsification cataract surgery (conventional group) in patients with hard nuclear cataract. SETTING: Eye Center, Second Affiliated Hospital of School of Medicine, Zhejiang University, Hangzhou, China. DESIGN: Prospective consecutive nonrandomized comparative cohort study. METHODS: According to the Emery-Little classification, cataract patients with a grade 4 or 5 hard nuclei were assigned to 1 of the 2 groups...
April 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28532810/species-level-identification-of-coagulase-negative-staphylococcus-spp-from-buffalo-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-and-cydb-real-time-quantitative-pcr
#17
Lucas J L Pizauro, Camila C de Almeida, Glenn A Soltes, Durda Slavic, Oswaldo D Rossi-Junior, Fernando A de Ávila, Luiz F Zafalon, Janet I MacInnes
Incorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28521677/molluscum-contagiosum-an-update
#18
Alexander Kc Leung, Benjamin Barankin, Kam Lun Hon
BACKGROUND: Molluscum contagiosum is a viral cutaneous infection in childhood that occurs worldwide. Physicians should familiarize themselves with this common condition. OBJECTIVE: To review in depth the epidemiology, pathophysiology, clinical manifestations, complications and, in particular, treatment of molluscum contagiosum. METHODS: A PubMed search was completed in Clinical Queries using the key term "molluscum contagiosum". Patents were searched using the key term "molluscum contagiosum" from www...
May 17, 2017: Recent Patents on Inflammation & Allergy Drug Discovery
https://www.readbyqxmd.com/read/28500259/central-serous-chorioretinopathy-secondary-to-tuberculosis-cause-or-coincidence
#19
Perwez Khan, Lubna Khan, Nikhat Anjum, Nutan Saxena
32-year-old male with diagnosis of central serous chorioretinopathy (CSCR) in both eyes and negative history of administration of any medicine was treated with bilateral focal laser at leakage point, with visual recovery. Recurrence occurred after 2 years in right eye and was treated successfully with acetazolamide. Second recurrence occurred after 1 year. Acetazolamide was restarted, but no improvement was noted. Thorough re-evaluation of the patient revealed a family history of tuberculosis. Ancillary investigations rendered the presumptive diagnosis of tuberculosis, and antituberculosis treatment led to visual recovery with no recurrences...
May 12, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28497661/medication-related-osteonecrosis-of-the-jaw-risk-factors-in-patients-under-biphosphonate-versus-patients-under-antiresorptive-antiangiogenic-drugs
#20
Giulia Ghidini, Maddalena Manfredi, Ilaria Giovannacci, Giovanni Mergoni, Amin Sarraj, Maura Mureddu, Giovanna Giunta, Mauro Bonanini, Marco Meleti, Paolo Vescovi
BACKGROUND: Biphosphonate-Related Osteonecrosis of the Jaw (BRONJ) is a potential side effect associated with the administration of bisphosphonates; the aim of this work is to highlight the possible epidemiological differences between two groups of patients affected by MRONJ treated at the Center of Oral Medicine, Pathology and Laser Surgery of the Academic Hospital at the University of Parma, Italy, between January 2004 and June 2016. METHODS: Medical charts of 303 patients (214 Females and 89 Males, mean age 67y) treated at the Center of Oral Medicine, Pathology and Laser Surgery of the Academic Hospital at the University of Parma, between January 2004 and June 2016, were retrospectively analyzed...
May 10, 2017: Minerva Stomatologica
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