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https://www.readbyqxmd.com/read/27924510/global-protein-expression-analysis-of-molecular-markers-of-ds-1-47-a-component-of-implantation-promoting-traditional-chinese-medicine
#1
Yan-Ling Li, Xiao-Yan Zhang, Yu Leng, Yan-Li Wu, Jing Li, Yun-Xia Wu
This study investigated the molecular markers of DS-1-47, a component of an implantation- promoting traditional Chinese medicine consisting of Astragalus mongholicus, Atractylodes macrocephala, Scutellaria baicalensis and Dipsacales, in an attempt to clarify the molecular mechanism and action targets of DS-1-47. Controlled ovarian stimulation (COS) method was used to establish the implantation dysfunction models of mice. Animals were divided into normal pregnant group, COS model group and DS-1-47 group. Laser capture microdissection-double dimensional electrophoresis-mass spectrum (LCM-DE-MS) was used to analyze the uterine protein molecules that were possibly involved in the promotion of implantation...
December 2016: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/27910289/visible-laser-induced-in-situ-cell-detachment-from-gold-nanoparticle-embedded-collagen-gel
#2
Chie Kojima, Yusuke Nakajima, Naoya Oeda, Takeshi Kawano, Yusuke Taki
Cell sorting is important for cell biology and regenerative medicine. A visible light-responsive cell scaffold is produced using gold nanoparticles and collagen gel. Various kinds of cells are cultured on the visible light-responsive cell scaffold, and the target cells are selectively detached by photoirradiation without any cytotoxicity. This is a new image-guided cell sorting system.
December 2, 2016: Macromolecular Bioscience
https://www.readbyqxmd.com/read/27897130/recommendations-for-potassium-titanyl-phosphate-laser-in-the-treatment-of-cholesteatoma
#3
REVIEW
Gavin John le Nobel, Adrian Lewis James
Potassium-titanyl-phosphage (KTP) lasers possess many characteristics suitable for otologic surgery. The objective of this report is to provide recommendations on the use of KTP laser for cholesteatoma surgery based on a narrative review of currently available evidence. PubMed and the Cochrane Review of randomized control trials were searched for relevant publications on efficacy and adverse effects and relevant articles appraised by the authors using Oxford Centre for Evidence-based Medicine criteria for recommendations...
November 28, 2016: Journal of International Advanced Otology
https://www.readbyqxmd.com/read/27889428/spectroscopic-and-e-tongue-evaluation-of-medicinal-plants-a-taste-of-how-rasa-can-be-studied
#4
Rama Jayasundar, Somenath Ghatak
BACKGROUND: The use of medicinal plants in Ayurveda is based on rasa, generally taken to represent taste as a sensory perception. This chemosensory parameter plays an important role in Ayurvedic pharmacology. OBJECTIVE: The aim is to explore the use of structuro-functional information deduced from analytical techniques for the rasa-based classification of medicinal plants in Ayurveda. MATERIALS AND METHODS: Methods of differential sensing and spectroscopic metabolomics have been used in select medicinal plants from three different taste categories (sweet, pungent and multiple taste): Tribulus terrestris, Vitis vinifera and Glycyrrhiza glabra from sweet category; Piper longum, Cuminum cyminum and Capsicum annum from pungent group; Emblica officinalis with five tastes...
November 23, 2016: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/27887850/laser-flow-cytometry-as-a-tool-for-the-advancement-of-clinical-medicine
#5
REVIEW
David Aebisher, Dorota Bartusik, Jacek Tabarkiewicz
Flow cytometry is a classic laser technology. With the discovery of the cytometer, flow cytometry has become a primary tool in biodiagnostic research. This review focuses on current applications of flow cytometry to the diagnosis of disease and treatment monitoring at the single-cell level. A description of the principles of flow cytometry and a brief overview of the major applications are presented. Our criteria for selecting research papers for this review are those that show advances in biomedicine and pharmacotherapy achieved by using non-invasive flow cytometry...
November 22, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27878794/varicosities-of-the-lower-extremity-new-approaches-cosmetic-or-therapeutic-needs
#6
Luca Spinedi, Heiko Uthoff, Sasan Partovi, Daniel Staub
Varicose veins of the lower extremity (VVLE) are a frequently encountered vascular disorder in the general population. The general view that VVLE are a non-serious disease with primarily aesthetic impact is a common misconception, as the disease can have a significant negative impact on generic and disease-specific quality of life. Further, VVLE may be associated with potentially threatening clinical conditions, such as chronic venous ulceration, venous thromboembolism and haemorrhage from ruptured varicose veins...
2016: Swiss Medical Weekly
https://www.readbyqxmd.com/read/27874198/crataeva-nurvala-nanoparticles-inhibit-virulence-factors-and-biofilm-formation-in-clinical-isolates-of-pseudomonas-aeruginosa
#7
Syed Ghazanfar Ali, Mohammad Azam Ansari, Haris M Khan, Mohammad Jalal, Abbas Ali Mahdi, Swaranjit Singh Cameotra
Green synthesized nanoparticles have gained great attention due to their non-toxic and non-hazardous nature. In the present study, bark extract of the medicinal plant in Ayurveda Crataeva nurvala (Buch-Ham) (CN) was chosen for the biosynthesis of silver nanoparticles (AgNPs). These NPs were characterized by Ultra violet visible spectroscopy, Fourier Transform Infra Red, Atomic Force Microscopy, and Transmission Electron Microscopy (TEM). The average particle size of green synthesized CN-AgNPs was 15.2 ± 1...
November 22, 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27872848/role-of-membrane-lipids-in-the-regulation-of-erythrocytic-oxygen-transport-function-in-cardiovascular-diseases
#8
Victor V Revin, Natalia V Gromova, Elvira S Revina, Maria I Martynova, Angelina I Seikina, Nadezhda V Revina, Oksana G Imarova, Ilia N Solomadin, Alexander Yu Tychkov, Nikolai Zhelev
The composition and condition of membrane lipids, the morphology of erythrocytes, and hemoglobin distribution were explored with the help of laser interference microscopy (LIM) and Raman spectroscopy. It is shown that patients with cardiovascular diseases (CVD) have significant changes in the composition of their phospholipids and the fatty acids of membrane lipids. Furthermore, the microviscosity of the membranes and morphology of the erythrocytes are altered causing disordered oxygen transport by hemoglobin...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27851876/modeling-diffusion-of-intracellular-metabolites-in-the-mouse-brain-up-to-very-high-diffusion-weighting-diffusion-in-long-fibers-almost-accounts-for-non-monoexponential-attenuation
#9
Marco Palombo, Clemence Ligneul, Julien Valette
PURPOSE: To investigate how intracellular metabolites diffusion measured in vivo up to very high q/b in the mouse brain can be explained in terms of simple geometries. METHODS: 10 mice were scanned using our new STE-LASER sequence, at 11.7 Tesla (T), up to qmax = 1 μm(-1) at diffusion time td = 63.2 ms, corresponding to bmax = 60 ms/µm². We model cell fibers as randomly oriented cylinders, with radius a and intracellular diffusivity Dintracyl, and fit experimental data as a function of q to estimate Dintracyl and a...
November 7, 2016: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/27828195/label-free-optical-sensor-based-on-red-blood-cells-laser-tweezers-raman-spectroscopy-analysis-for-abo-blood-typing
#10
Duo Lin, Zuci Zheng, Qiwen Wang, Hao Huang, Zufang Huang, Yun Yu, Sufang Qiu, Cuncheng Wen, Min Cheng, Shangyuan Feng
The clinical significance of ABO blood typing extends beyond transfusion medicine and is demonstrated to be associated with susceptibility to various diseases, even including cancer. In this study, a home-made laser tweezers Raman spectroscopy (LTRS) system was applied to detect red blood cells (RBCs) with the aim to develop a label-free, simple and objective blood typing method for the first time. High-quality Raman spectra of RBCs in the fingerprint region of 420-1700 cm<sup>-1</sup> can be obtained, meanwhile exciting blood typing results can be achieved, especially with an accuracy of 100% for identifying Type AB from other blood types with the use of multivariate statistical analysis based on principal component analysis (PCA) combined with linear discriminant analysis (LDA)...
October 17, 2016: Optics Express
https://www.readbyqxmd.com/read/27824072/superparamagnetic-fe3o4-peg2k-fa-ce6-nanoprobes-for-in-vivo-dual-mode-imaging-and-targeted-photodynamic-therapy
#11
Ting Yin, Peng Huang, Guo Gao, Joseph G Shapter, Yulan Shen, Rongjin Sun, Caixia Yue, Chunlei Zhang, Yanlei Liu, Sui Zhou, Daxiang Cui
The development of targeted nanoprobes is a promising approach to cancer diagnostics and therapy. In the present work, a novel multifunctional photo/magnet-diagnostic nanoprobe (MNPs-PEG2K-FA@Ce6) has been developed. This nanoprobe is built using folic acid (FA), bifunctional polyethylene glycol (PEG2K) and photosensitizer chlorin e6 (Ce6). The MNPs-PEG2K-FA@Ce6 nanoprobes are superparamagnetic, can be synthesized on a large scale by a one-pot hydrothermal process without further surface modification and are stable in an aqueous environment for eight months...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27816913/laser-acupuncture-treatment-improves-pain-and-functional-status-in-patients-with-subacromial-impingement-syndrome-a-randomized-double-blind-sham-controlled-study
#12
Sibel Kibar, Hatice Ecem Konak, Deniz Evcik, Saime Ay
OBJECTIVE:  The present study aims to determine the effect of laser acupuncture in patients with subacromial impingement syndrome (SAIS). DESIGN:  Randomized, double-blind, sham-controlled study. SETTING:  Physical medicine and rehabilitation outpatient clinic. SUBJECTS:  A total 73 patients with SAIS were randomly assigned into a treatment group (n = 36) and a control group (n = 37). METHODS : The treatment group received laser acupuncture, and the control group received sham laser acupuncture...
November 5, 2016: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/27815610/matrix-assisted-laser-desorption-ionization-imaging-mass-spectrometry-reveals-changes-of-phospholipid-distribution-in-induced-pluripotent-stem-cell-colony-differentiation
#13
Yasuo Shimizu, Motoyasu Satou, Keitaro Hayashi, Yusuke Nakamura, Mio Fujimaki, Yasuhiro Horibata, Hiromi Ando, Taiji Watanabe, Taichi Shiobara, Kazuyuki Chibana, Akihiro Takemasa, Hiroyuki Sugimoto, Naohiko Anzai, Yoshiki Ishii
Induced pluripotent stem cells (iPSCs) are opening up new possibilities for medicine. Understanding the regulation of iPSC biology is important when attempting to apply these cells to disease models or therapy. Changes of lipid metabolism in iPSCs were investigated by matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF-IMS). Analysis revealed changes of the intensity and distribution of peaks at m/z 782.5 and 798.5 in iPSC colonies during spontaneous differentiation...
November 4, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27812591/photochemical-formation-of-quinone-methides-from-peptides-containing-modified-tyrosine
#14
Antonija Husak, Benjamin P Noichl, Tatjana Šumanovac Ramljak, Margareta Sohora, Đani Škalamera, Nediljko Budiša, Nikola Basarić
We have demonstrated that quinone methide (QM) precursors can be introduced in the peptide structure and used as photoswitchable units for peptide modifications. QM precursor 1 was prepared from protected tyrosine in the Mannich reaction, and further used as a building block in peptide synthesis. Moreover, peptides containing tyrosine can be transformed into a photoactivable QM precursor by the Mannich reaction which can afford monosubstituted derivatives 2 or bis-substituted derivatives 3. Photochemical reactivity of modified tyrosine 1 and dipeptides 2 and 3 was studied by preparative irradiation in CH3OH where photodeamination and photomethanolysis occur...
November 4, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27788183/angle-closure-scoring-system-acss-a-scoring-system-for-stratification-of-angle-closure-disease
#15
Aparna Rao, Debananda Padhy, Sarada Sarangi, Gopinath Das
PURPOSE: To evaluate the angle closure scoring system (ACSS) for stratifying primary angle course disease. METHODS: This observational cross sectional institutional study included patients with primary open angle glaucoma suspects (n = 21) and primary angle closure disease (primary angle closure, PAC, n = 63 and primary angle course glaucoma, PACG, n = 58 (defined by International society of Geographical and Epidemiological Ophthalmology, ISGEO). Two independent examiners blinded to clinical details, graded good quality pre-laser goniophotographs of the patients incorporating quadrants of peripheral anterior synechieae (PAS), non-visibility of posterior trabecular meshwork (PTM) and blotchy pigments (ranging from 1-4 quadrants), iris configuration, angle recess (sum of above depicting ACSSg) and lens thickness/axial length ratio (LT/AL), cup disc ratio and baseline intraocular pressure (IOP) to give total score (ACSSt)...
2016: PloS One
https://www.readbyqxmd.com/read/27780304/news-announcements-american-society-for-laser-medicine-and-surgery-inc
#16
(no author information available yet)
No abstract text is available yet for this article.
November 2016: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/27770937/evaluation-of-the-potential-of-rhtgf-%C3%AE-3-encapsulated-p-lla-cl-collagen-nanofibers-for-tracheal-cartilage-regeneration-using-mesenchymal-stems-cells-derived-from-wharton-s-jelly-of-human-umbilical-cord
#17
Jing Wang, Binbin Sun, Lingling Tian, Xiaomin He, Qiang Gao, Tong Wu, Seeram Ramakrishna, Jinghao Zheng, Xiumei Mo
Tracheal injuries are one of major challenging issues in clinical medicine because of the poor intrinsic ability of tracheal cartilage for repair. Tissue engineering provides an alternative method for the treatment of tracheal defects by generating replacement tracheal structures. In this study, core-shell nanofibrous scaffold was fabricated to encapsulate bovine serum albumin & rhTGF-β3 (recombinant human transforming growth factor-β3) into the core of the nanofibers for tracheal cartilage regeneration. Characterization of the core-shell nanofibrous scaffold was carried out by scanning electron microscope (SEM), transmission electron microscope (TEM), laser scanning confocal microscopy (LSCM), and tensile mechanical test...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27755130/twin-pregnancy-complicated-by-selective-growth-restriction
#18
Rosemary Townsend, Asma Khalil
PURPOSE OF REVIEW: Selective intrauterine growth restriction (sIUGR) in twin pregnancies presents a complex management problem for the fetal medicine specialist. This review summarizes what is currently known about sIUGR pathophysiology, particularly in monochorionic pregnancies and looks at the results of early trials of fetal intervention in high-risk sIUGR pregnancies. RECENT FINDINGS: sIUGR in monochorionic pregnancies is due to unequal placental sharing, but the clinical outcome is determined by the characteristics of the placental vascular anastomoses...
October 15, 2016: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27754561/news-announcements-american-society-for-laser-medicine-and-surgery-inc
#19
(no author information available yet)
No abstract text is available yet for this article.
October 2016: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/27754252/os-36-03-e-box-cpg-hypomethylation-of-nmn-producing-enzyme-nampt-in-proximal-tubules-enables-constant-supply-of-nmn-from-tubules-to-glomeruli-which-is-disrupted-in-diabetic-nephropathy
#20
Hirokazu Muraoka, Kazuhiro Hasegawa, Shu Wakino
OBJECTIVE: Nicotinamide phosphoribosyltransferase (Nampt) cooperates with a longevity gene, Sirt1, to exert the potentiation of stress resistance. We have reported that the high expression level of Nampt in PTs contributes to the sufficient supply of NMN to glomeruli. We also showed that downregulation of Nampt and NMN in PT in DN leads to glomerular damages (Nature Medicine 2013). However, the regulation mechanisms of Nampt expression remain unknown. DESIGN AND METHOD: We investigated how Nampt expression is consistently retained at high levels in PTs under normal conditions and how Nampt levels are decreased in DN...
September 2016: Journal of Hypertension
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