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Jinjie Wang, Xiangyi Huang, Heng Liu, Chaoqing Dong, Jicun Ren
In this work, we propose fluorescence and scattering light cross-correlation spectroscopy (FSCCS) based on laser confocal configuration using silver nanoparticle (SNPs) and Alexa Fluor 488 (Alexa) as probe pairs. FSCCS is a single molecule (particle) method, and its principle is similar to that of fluorescence cross-correlation spectroscopy (FCCS). We established the setup of FSCCS using single wavelength laser and developed an immunoassay model of FSCCS. The reliability and adaptability of FSCCS method were evaluated by homogeneous sandwich immunoassay mode...
May 2, 2017: Analytical Chemistry
Douglas M Brown
Pablo Picasso (1881-1973) is quoted as saying, "The meaning of life is to find your gift. The purpose of life is to give it away." With these few words Picasso captures the essence of David Drum's existence. From his birth in Moncton, NB, through his formal education in Toronto, ON, to his so-called "retirement" in Crystal Beach, ON, this paper spans 72 years, unveiling the broad range of opportunities for growth David Drum has encountered in his life's journey and documenting his acceptance of the obligations for proud philanthropy that accompany them...
March 2017: Journal of the Canadian Chiropractic Association
Ming Chen Ba, Hui Long, Xiang Liang Zhang, Yuan Feng Gong, Zhao Fei Yan, Shuai Wang, Yun Qiang Tang, Shu Zhong Cui
PURPOSE: CO₂ leakage along the trocar (chimney effect) has been proposed to be an important factor underlying port-site metastasis after laparoscopic surgery. This study aimed to test this hypothesis by comparing the incidence of port-site metastasis between B-ultrasound-guided and laparoscopically-assisted hyperthermic intraperitoneal perfusion chemotherapy (HIPPC). MATERIALS AND METHODS: Sixty-two patients with malignant ascites induced by gastrointestinal or ovarian cancer were divided into two groups to receive either B-ultrasound-guided or laparoscopically-assisted HIPPC...
May 2017: Yonsei Medical Journal
Abhishek Kumar, G V Shivashankar
Mechanical coupling between the nucleus and the cytoskeleton is indispensable for direct force transduction from the extra cellular matrix (ECM) to the chromatin. Although this physical coupling has been shown to be crucial for nuclear positioning and its function, the quantification of nuclear-cytoskeleton interaction has been lacking. In this paper, using various quantitative fluorescence spectroscopy techniques, we investigate the nature of this connection. High-resolution 3D imaging shows that nesprin2G forms short linear structures along actin stress fibers (ASFs) in the apical region of the nucleus...
November 11, 2016: Methods and Applications in Fluorescence
Na Li, Gui-Wen Huang, Yuan-Qing Li, Hong-Mei Xiao, Qing-Ping Feng, Ning Hu, Shao-Yun Fu
It is well accepted that the microwave absorption performance (MAP) of carbon nanotubes (CNTs) can be enhanced via coating magnetic nanoparticles on their surfaces. However, it is still unclear if the magnetic coating structure has a significant influence on the microwave absorption behavior. In this work, nano-Fe3O4 compact-coated CNTs (FCCs) and Fe3O4 loose-coated CNTs (FLCs) are prepared using a simple solvothermal method. The MAP of the Fe3O4-coated CNTs is shown to be adjustable via controlling the Fe3O4 nanocoating structure...
January 9, 2017: ACS Applied Materials & Interfaces
Misato Ohtani, Nobuhiro Akiyoshi, Yuto Takenaka, Ryosuke Sano, Taku Demura
One crucial problem that plants faced during their evolution, particularly during the transition to growth on land, was how to transport water, nutrients, metabolites, and small signaling molecules within a large, multicellular body. As a solution to this problem, land plants developed specific tissues for conducting molecules, called water-conducting cells (WCCs) and food-conducting cells (FCCs). The well-developed WCCs and FCCs in extant plants are the tracheary elements and sieve elements, respectively, which are found in vascular plants...
January 2017: Journal of Experimental Botany
Stanisław Pieczarkowski, Kinga Kowalska-Duplaga, Przemko Kwinta, Przemysław Tomasik, Andrzej Wędrychowicz, Krzysztof Fyderek
Objectives. The aim of the study was to establish whether fecal calprotectin concentration (FCC) may be useful in children with chronic abdominal pain to differentiate between inflammatory bowel disease (IBD), other inflammatory gastrointestinal disorders, and functional gastrointestinal disorders. Methods. The study included 163 patients (median age 13 years), who were assigned to four study groups: group 0 (control), 22 healthy children; group 1, 33 children with functional gastrointestinal disorders; group 2, 71 children with inflammatory gastrointestinal disorders other than IBD; group 3, 37 children with IBD...
2016: Gastroenterology Research and Practice
Mary Clare McCorry, Lawrence J Bonassar
Mesenchymal stem cells (MSCs) have been investigated with promising results for meniscus healing and tissue engineering. While MSCs are known to contribute to extracellular matrix (ECM) production, less is known about how MSCs produce and align large organized fibers for application to tissue engineering the meniscus. The goal of this study was to investigate the capability of MSCs to produce and organize ECM molecules compared to meniscal fibrochondrocytes (FCCs). Bovine FCCs and MSCs were encapsulated in an anatomically accurate collagen meniscus using monoculture and co-culture of each cell type...
December 7, 2016: Connective Tissue Research
Sibel Yavas, Radek Macháň, Thorsten Wohland
The epidermal growth factor receptor (EGFR) is a prototypical receptor tyrosine kinase involved in cell growth and proliferation and associated with various cancers. It is commonly assumed that after activation by binding of epidermal growth factor to the extracellular domain it dimerizes, followed by autophosphorylation of tyrosine residues at the intracellular domain. However, its oligomerization state before activation is controversial. In the absence of ligands, EGFR has been found in various, inconsistent amounts of monomeric, inactive dimeric, and oligomeric forms...
November 15, 2016: Biophysical Journal
Marian Von-Maszewski, Gregory Botz
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
Catherine Verrier Piersol, E Adel Herge, Albert E Copolillo, Benjamin E Leiby, Laura N Gitlin
Caregiver appraisal influences care decisions and may place the person with dementia at risk. The Functional Capacity Card Sort (FCCS) was developed to determine caregiver appraisal. The objective of this study was to examine FCCS psychometric properties and utility, using data from a cross-sectional study (N = 86). The FCCS had moderately positive association (convergent validity) with a related activities of daily living (ADL) index (r = .43, p < .0001), weak negative association (discriminant validity) with an unrelated neuropsychiatric symptom index (r = -...
July 2016: OTJR: Occupation, Participation and Health
Yawei Dai, Markus Seeger, Jingwei Weng, Song Song, Wenning Wang, Yan-Wen Tan
Cellular informational and metabolic processes are propagated with specific membrane fusions governed by soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNARE). SNARE protein Ykt6 is highly expressed in brain neurons and plays a critical role in the membrane-trafficking process. Studies suggested that Ykt6 undergoes a conformational change at the interface between its longin domain and the SNARE core. In this work, we study the conformational state distributions and dynamics of rat Ykt6 by means of single-molecule Förster Resonance Energy Transfer (smFRET) and Fluorescence Cross-Correlation Spectroscopy (FCCS)...
2016: Scientific Reports
Honorio Vergara-Domínguez, José Julían Ríos, Beatriz Gandul-Rojas, María Roca
The central reaction of chlorophyll (chl) breakdown pathway occurring during olive fruits maturation is the cleavage of the macrocycle pheophorbide a to a primary fluorescent chl catabolite (pFCC) and it is catalyzed by two enzymes: pheophorbide a oxygenase (PaO) and red chl catabolite reductase (RCCR). In subsequent steps, pFCC is converted to different fluorescent chlorophyll catabolites (FCCs) and nonfluorescent chlorophyll catabolites (NCCs). This work demonstrated that RCCR activity of olive fruits is type II...
December 1, 2016: Food Chemistry
Anthony Olufemi Eshofonie, Huai Lin, Randy Paul Valcin, Darrel Irvin, Kathleen Goss, Jeremy Piper
Timely reporting of disease outbreak and access to the outbreak site are necessary for prompt investigation and control. Local health departments (LHDs) establish the reporting time frames, methods, and communication channels within their jurisdictions in advance. This report describes a salmonellosis outbreak investigation in a federal correctional center (FCC) that was delayed due to issues with jurisdictional ownership and with separate reporting protocols for the FCC. The setting of the outbreak within an FCC posed additional unique challenges to the outbreak investigation...
July 2016: Journal of Correctional Health Care
Daria Kotlarek, Remigiusz Worch
PA subunit of influenza RNA-dependent RNA polymerase deserves constantly increasing attention due to its essential role in influenza life cycle. N-terminal domain of PA (PA-Nter) harbors endonuclease activity, which is indispensable in viral transcription and replication. Interestingly, existing literature reports on in vitro ion preferences of the enzyme are contradictory. Some show PA-Nter activity exclusively with Mn2+, whereas others report Mg2+ as a natural cofactor. To clarify it, we performed a series of experiments with varied ion concentrations and substrate type...
2016: PloS One
Elizabeth Barty, Katy Caynes, Leanne M Johnston
AIM: This paper describes the development, validation, and reliability of the Functional Communication Classification System (FCCS), designed to classify expressive communication skills of children with cerebral palsy (CP) aged 4 years and 5 years (between their fourth and sixth birthdays). METHOD: The Functional Communication Classification System (FCCS) was developed in 2006 using a literature review, client file audit, and expert consultative committee process in order to devise scale content, structure, and check clinical validity and utility...
October 2016: Developmental Medicine and Child Neurology
Sherri L LaVela, Stephanie Turcios, Alex Malhiot, Bella Etingen, Jennifer N Hill, Scott Miskevics
INTRODUCTION: Family-centered care (FCC) involves partnering with patients and family/caregivers ("caregivers"), engaging them in decisions, and providing support. FCC may be measured across 3 dimensions: respect, collaboration, and support and needs likely vary by caregiver age. The objective was to examine and compare caregiver perceptions of FCC by age. METHOD: We mailed surveys to family/caregivers of Veterans. We collected demographics and measured respect, collaboration, and support using the Family-Centered Care Survey (FCCS)...
June 2016: Families, Systems & Health: the Journal of Collaborative Family Healthcare
Mary Clare McCorry, Jennifer L Puetzer, Lawrence J Bonassar
BACKGROUND: Bone marrow mesenchymal stem cells (MSCs) have shown positive therapeutic effects for meniscus regeneration and repair. Preliminary in vitro work has indicated positive results for MSC applications for meniscus tissue engineering; however, more information is needed on how to direct MSC behavior. The objective of this study was to examine the effect of MSC co-culture with primary meniscal fibrochondrocytes (FCCs) in a three-dimensional collagen scaffold in fibrochondrogenic media...
March 12, 2016: Stem Cell Research & Therapy
Thomas Antoine, David Ott, Katharina Ebell, Kerrin Hansen, Luc Henry, Frank Becker, Stefan Hannus
G protein-coupled receptors (GPCRs) mediate many important physiological functions and are considered as one of the most successful therapeutic target classes for a wide spectrum of diseases. Drug discovery projects generally benefit from a broad range of experimental approaches for screening compound libraries and for the characterization of binding modes of drug candidates. Owing to the difficulties in solubilizing and purifying GPCRs, assay formats have been so far mainly limited to cell-based functional assays and radioligand binding assays...
June 1, 2016: Analytical Biochemistry
Isabela Costa Guimarães, Kelen Cristina dos Reis, Evandro Galvão Tavares Menezes, Paulo Rogério Siriano Borges, Ariel Costa Rodrigues, Renato Leal, Thais Hernandes, Elisângela Helena Nunes de Carvalho, Eduardo Valério de Barros Vilas Boas
This work evaluates fresh-cut carrots (FCC) coated with montmorillonite (MMT) subjected to passive modified atmosphere packaging. Carrots were sanitized, cooled, peeled and sliced. Half of the FCC were coated with MMT nanoparticle film and the other half were not. All FCCs were packed in a polypropylene rigid tray, covered with a polypropylene rigid lid or sealed with polyethylene + propylene film, in four treatments (RL, rigid lid; RLC, rigid lid + coating; ST, sealed tray; STC, sealed tray + coating)...
2016: International Journal of Food Sciences and Nutrition
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