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https://www.readbyqxmd.com/read/29456673/full-thickness-conjunctival-flap-covering-surgery-combined-with-amniotic-membrane-transplantation-for-severe-fungal-keratitis
#1
Jing Zhong, Bowen Wang, Saiqun Li, Yuqing Deng, Haixiang Huang, Ling Chen, Jin Yuan
Fungal keratitis is a severe and common ocular infectious disease. The present study evaluated the efficiency of full-thickness conjunctival flap covering surgery with amniotic membrane transplantation (FCCS + AMT) as a transitional surgery for severe fungal keratitis. A total of 17 patients with severe fungal keratitis without corneal perforation underwent FCCS + AMT between January 2010 and December 2015. The pathogenic factors, preoperative diagnosis and postoperative prognosis of FCCS + AMT at 3 months were evaluated...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29448037/quantifying-membrane-protein-oligomerization-with-fluorescence-cross-correlation-spectroscopy
#2
Megan J Kaliszewski, Xiaojun Shi, Yixuan Hou, Ryan Lingerak, Soyeon Kim, Paul Mallory, Adam W Smith
Fluorescence cross-correlation spectroscopy (FCCS) is an advanced fluorescence technique that can quantify protein-protein interactions in vivo. Due to the dynamic, heterogeneous nature of the membrane, special considerations must be made to interpret FCCS data accurately. In this study, we describe a method to quantify the oligomerization of membrane proteins tagged with two commonly used fluorescent probes, mCherry (mCH) and enhanced green (eGFP) fluorescent proteins. A mathematical model is described that relates the relative cross-correlation value (f c ) to the degree of oligomerization...
February 12, 2018: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29397632/conventional-cytology-vs-immunocytochemistry-of-intraoperative-peritoneal-washes-in-gastric-cancer-patients-subjected-to-gastrectomy-clinical-correlates-and-association-with-overall-survival
#3
Tomasz Olesiński, Małgorzata Malinowska, Maria Zwierko, Katarzyna Pogoda, Andrzej Rutkowski, Marek Szpakowski, Piotr Saramak, Anna Nasierowska-Guttmejer
BACKGROUND: The aim of this study was to verify if positive results yielded with conventional cytology and immunocytochemical analysis of peritoneal washes correlate with established prognostic factors and overall survival (OS) in gastric cancer patients. METHODS: The study included the data of 271 gastrectomized patients. Peritoneal washes of 131 (48.3%) patients were examined by means of conventional cytology, and material from 140 (51.7%) subjects treated surgically after this date was subjected to immunocytochemical analysis...
February 1, 2018: Minerva Chirurgica
https://www.readbyqxmd.com/read/29290039/evidence-of-g-protein-coupled-receptor-and-substrate-transporter-heteromerization-at-a-single-molecule-level
#4
Jana Fischer, Gunnar Kleinau, Claudia Rutz, Denise Zwanziger, Noushafarin Khajavi, Anne Müller, Maren Rehders, Klaudia Brix, Catherine L Worth, Dagmar Führer, Heiko Krude, Burkhard Wiesner, Ralf Schülein, Heike Biebermann
G-protein-coupled receptors (GPCRs) can constitute complexes with non-GPCR integral membrane proteins, while such interaction has not been demonstrated at a single molecule level so far. We here investigated the potential interaction between the thyrotropin receptor (TSHR) and the monocarboxylate transporter 8 (MCT8), a member of the major facilitator superfamily (MFS), using fluorescence cross-correlation spectroscopy (FCCS). Both the proteins are expressed endogenously on the basolateral plasma membrane of the thyrocytes and are involved in stimulation of thyroid hormone production and release...
December 30, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29285609/functional-compromise-cohort-study-fccs-sarcopenia-is-a-strong-predictor-of-mortality-in-the-intensive-care-unit
#5
P A de Hoogt, K W Reisinger, J J W Tegels, J W A M Bosmans, F Tijssen, J H M B Stoot
BACKGROUND: Functional compromise in elderly patients is considered to be a significant contributing factor in increased postoperative morbidity and mortality. It is described as a state of reduced physiologic reserves including, e.g., sarcopenia, cachexia, malnutrition and frailty with increased susceptibility to adverse health outcomes. Aim of this study was to investigate the association of sarcopenia with mortality in ICU patients. METHODS: A retrospective analysis of a total of 687 patients admitted to the ICU from January 2013 until December 2014 was performed...
December 29, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/29284551/family-care-conferences-in-long-term-care-exploring-content-and-processes-in-end-of-life-communication
#6
Pamela Durepos, Sharon Kaasalainen, Tamara Sussman, Deborah Parker, Kevin Brazil, Susan Mintzberg, Alyssa Te
OBJECTIVE: End-of-life (EoL) communication in long-term care (LTC) homes is often inadequate and delayed, leaving residents dying with unknown preferences or goals of care. Poor communication with staff contributes to families feeling unprepared, distressed, and dissatisfied with care. Family care conferences (FCCs) aim to increase structured systematic communication around goals and plans for the end of life. As part of the Strengthening a Palliative Approach to Care (SPA-LTC) project, FCCs were implemented in four LTC sites in Ontario, Canada...
December 29, 2017: Palliative & Supportive Care
https://www.readbyqxmd.com/read/29250810/complementary-version-of-fermion-coupled-coherent-states-method-and-gram-schmidt-algorithm-theory-and-applications-for-electronic-states-of-h2-and-h2
#7
Mohammadreza Eidi, Mohsen Vafaee, Mitra Rooein
In our previous report, we introduced a new version of Fermion coupled coherent states method (FCCS) which was especially suited for simulating the first symmetric spatial electronic state of two-electron systems. In this manuscript, we report a complementary version for FCCS method to simulate both of the first symmetric and antisymmetric spatial electronic states of two-electron systems. Moreover, the Gram-Schmidt orthogonalization process is employed to reach the excited states of the system. We apply this FCCS method and the original coupled coherent state method to simulate the energy of different electronic states of H2 and H2+, respectively...
December 18, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/29155588/determination-of-equilibrium-constant-and-relative-brightness-in-fret-fcs-by-including-the-third-order-correlations
#8
Lingyi Meng, Shanshan He, Xin Sheng Zhao
Fluorescence correlation spectroscopy (FCS) encodes the information of the equilibrium constant (K), the relative fluorescence brightness of fluorophore (Q), and the forward and backward reaction rate constants (k+ and k-) on a physical or chemical relaxation. However, it has been a long-standing problem to completely resolve the FCS data to get the thermodynamic and kinetic information. Recently, we have solved the problem for fluorescence auto-correlation spectroscopy (FACS). Here, we extend the method to fluorescence cross-correlation spectroscopy (FCCS), which appears when FCS is coupled with fluorescence resonance energy transfer (FRET)...
November 20, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29065739/fecal-calprotectin-in-management-of-clostridium-difficile-infection-a-longitudinal-study
#9
Antonella Gallo, Carla Vallone, Ludovico Sabatelli, Giulio Ventura, Marcello Covino, Giovanni Cammarota, Antonio Gasbarrini, Raffaele Landolfi, Massimo Montalto
BACKGROUND: Clostridium difficile infection (CDI) is characterized by a relevant intestinal neutrophil infiltrate. So far, role of fecal calprotectin in CDI, has been investigated only in few studies, mainly focused on diagnosis of the disease. AIM: By a longitudinal design, we assess fecal calprotectin concentrations (FCCs) in subjects with CDI, evaluating the correlation between fecal marker and response to therapy. METHODS: Clinical (diarrhea scoring) and laboratory (FCCs and leucocytes count) evaluation was performed in 56 subjects with CDI at time of diagnosis (T0) and after a week from starting of therapy (T1)...
October 24, 2017: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/28962904/simultaneous-membrane-interaction-of-amphipathic-peptide-monomers-self-aggregates-and-cargo-complexes-detected-by-fluorescence-correlation-spectroscopy
#10
Luís Vasconcelos, Tõnis Lehto, Fatemeh Madani, Vlad Radoi, Mattias Hällbrink, Vladana Vukojević, Ülo Langel
Peptides able to translocate cell membranes while carrying macromolecular cargo, as cell-penetrating peptides (CPPs), can contribute to the field of drug delivery by enabling the transport of otherwise membrane impermeable molecules. Formation of non-covalent complexes between amphipathic peptides and oligonucleotides is driven by electrostatic and hydrophobic interactions. Here we investigate and quantify the coexistence of distinct molecular species in multiple equilibria, namely peptide monomer, peptide self-aggregates and peptide/oligonucleotide complexes...
September 26, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28893723/scientific-challenges-in-the-risk-assessment-of-food-contact-materials
#11
Jane Muncke, Thomas Backhaus, Birgit Geueke, Maricel V Maffini, Olwenn Viviane Martin, John Peterson Myers, Ana M Soto, Leonardo Trasande, Xenia Trier, Martin Scheringer
BACKGROUND: Food contact articles (FCAs) are manufactured from food contact materials (FCMs) that include plastics, paper, metal, glass, and printing inks. Chemicals can migrate from FCAs into food during storage, processing, and transportation. Food contact materials' safety is evaluated using chemical risk assessment (RA). Several challenges to the RA of FCAs exist. OBJECTIVES: We review regulatory requirements for RA of FCMs in the United States and Europe, identify gaps in RA, and highlight opportunities for improving the protection of public health...
September 11, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28740447/breast-dynamic-contrast-enhanced-mri-fibrocystic-changes-presenting-as-a-non-mass-enhancement-mimicking-malignancy
#12
Zorica C Milosevic, Mirjan M Nadrljanski, Zorka M Milovanovic, Nina Z Gusic, Slavko S Vucicevic, Olga S Radulovic
BACKGROUND: We aimed to analyse the morphokinetic features of breast fibrocystic changes (nonproliferative lesions, proliferative lesions without atypia and proliferative lesions with atypia) presenting as a non-mass enhancement (NME)in dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) examination. PATIENTS AND METHODS: Forty-six patients with histologically proven fibrocystic changes (FCCs) were retrospectively reviewed, according to Breast Imaging Reporting and Data System (BI-RADS) lexicon...
June 2017: Radiology and Oncology
https://www.readbyqxmd.com/read/28720130/psychosocial-and-behavioral-impact-of-breast-cancer-risk-assessed-by-testing-for-common-risk-variants-protocol-of-a-prospective-study
#13
Tatiane Yanes, Bettina Meiser, Mary-Anne Young, Rajneesh Kaur, Gillian Mitchell, Kristine Barlow-Stewart, Tony Roscioli, Jane Halliday, Paul James
BACKGROUND: The 'common variant, common disease' model predicts that a significant component of hereditary breast cancer unexplained by pathogenic variants in moderate or high-penetrance genes is due to the cumulative effect of common risk variants in DNA (polygenic risk). Assessing a woman's breast cancer risk by testing for common risk variants can provide useful information for women who would otherwise receive uninformative results by traditional monogenic testing. Despite increasing support for the utility of common risk variants in hereditary breast cancer, research findings have not yet been integrated into clinical practice...
July 18, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28436659/fluorescence-and-scattering-light-cross-correlation-spectroscopy-and-its-applications-in-homogeneous-immunoassay
#14
Jinjie Wang, Xiangyi Huang, Heng Liu, Chaoqing Dong, Jicun Ren
In this work, we propose fluorescence and scattering light cross-correlation spectroscopy (FSCCS) based on laser confocal configuration using silver nanoparticle (SNPs) and Alexa Fluor 488 (Alexa) as probe pairs. FSCCS is a single molecule (particle) method, and its principle is similar to that of fluorescence cross-correlation spectroscopy (FCCS). We established the setup of FSCCS using single wavelength laser and developed an immunoassay model of FSCCS. The reliability and adaptability of FSCCS method were evaluated by homogeneous sandwich immunoassay mode...
May 2, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28413227/david-c-drum-dc-fccs-c-an-accomplished-multi-faceted-individual
#15
Douglas M Brown
Pablo Picasso (1881-1973) is quoted as saying, "The meaning of life is to find your gift. The purpose of life is to give it away." With these few words Picasso captures the essence of David Drum's existence. From his birth in Moncton, NB, through his formal education in Toronto, ON, to his so-called "retirement" in Crystal Beach, ON, this paper spans 72 years, unveiling the broad range of opportunities for growth David Drum has encountered in his life's journey and documenting his acceptance of the obligations for proud philanthropy that accompany them...
March 2017: Journal of the Canadian Chiropractic Association
https://www.readbyqxmd.com/read/28332353/port-site-metastases-and-chimney-effect-of-b-ultrasound-guided-and-laparoscopically-assisted-hyperthermic-intraperitoneal-perfusion-chemotherapy
#16
Ming Chen Ba, Hui Long, Xiang Liang Zhang, Yuan Feng Gong, Zhao Fei Yan, Shuai Wang, Yun Qiang Tang, Shu Zhong Cui
PURPOSE: CO₂ leakage along the trocar (chimney effect) has been proposed to be an important factor underlying port-site metastasis after laparoscopic surgery. This study aimed to test this hypothesis by comparing the incidence of port-site metastasis between B-ultrasound-guided and laparoscopically-assisted hyperthermic intraperitoneal perfusion chemotherapy (HIPPC). MATERIALS AND METHODS: Sixty-two patients with malignant ascites induced by gastrointestinal or ovarian cancer were divided into two groups to receive either B-ultrasound-guided or laparoscopically-assisted HIPPC...
May 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/28192301/dynamic-interaction-between-actin-and-nesprin2-maintain-the-cell-nucleus-in-a-prestressed-state
#17
Abhishek Kumar, G V Shivashankar
Mechanical coupling between the nucleus and the cytoskeleton is indispensable for direct force transduction from the extra cellular matrix (ECM) to the chromatin. Although this physical coupling has been shown to be crucial for nuclear positioning and its function, the quantification of nuclear-cytoskeleton interaction has been lacking. In this paper, using various quantitative fluorescence spectroscopy techniques, we investigate the nature of this connection. High-resolution 3D imaging shows that nesprin2G forms short linear structures along actin stress fibers (ASFs) in the apical region of the nucleus...
November 11, 2016: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28025890/enhanced-microwave-absorption-performance-of-coated-carbon-nanotubes-by-optimizing-the-fe3o4-nanocoating-structure
#18
Na Li, Gui-Wen Huang, Yuan-Qing Li, Hong-Mei Xiao, Qing-Ping Feng, Ning Hu, Shao-Yun Fu
It is well accepted that the microwave absorption performance (MAP) of carbon nanotubes (CNTs) can be enhanced via coating magnetic nanoparticles on their surfaces. However, it is still unclear if the magnetic coating structure has a significant influence on the microwave absorption behavior. In this work, nano-Fe3O4 compact-coated CNTs (FCCs) and Fe3O4 loose-coated CNTs (FLCs) are prepared using a simple solvothermal method. The MAP of the Fe3O4-coated CNTs is shown to be adjustable via controlling the Fe3O4 nanocoating structure...
January 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28013230/evolution-of-plant-conducting-cells-perspectives-from-key-regulators-of-vascular-cell-differentiation
#19
REVIEW
Misato Ohtani, Nobuhiro Akiyoshi, Yuto Takenaka, Ryosuke Sano, Taku Demura
One crucial problem that plants faced during their evolution, particularly during the transition to growth on land, was how to transport water, nutrients, metabolites, and small signaling molecules within a large, multicellular body. As a solution to this problem, land plants developed specific tissues for conducting molecules, called water-conducting cells (WCCs) and food-conducting cells (FCCs). The well-developed WCCs and FCCs in extant plants are the tracheary elements and sieve elements, respectively, which are found in vascular plants...
January 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27974886/diagnostic-value-of-fecal-calprotectin-s100-a8-a9-test-in-children-with-chronic-abdominal-pain
#20
Stanisław Pieczarkowski, Kinga Kowalska-Duplaga, Przemko Kwinta, Przemysław Tomasik, Andrzej Wędrychowicz, Krzysztof Fyderek
Objectives. The aim of the study was to establish whether fecal calprotectin concentration (FCC) may be useful in children with chronic abdominal pain to differentiate between inflammatory bowel disease (IBD), other inflammatory gastrointestinal disorders, and functional gastrointestinal disorders. Methods. The study included 163 patients (median age 13 years), who were assigned to four study groups: group 0 (control), 22 healthy children; group 1, 33 children with functional gastrointestinal disorders; group 2, 71 children with inflammatory gastrointestinal disorders other than IBD; group 3, 37 children with IBD...
2016: Gastroenterology Research and Practice
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