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https://www.readbyqxmd.com/read/28962904/simultaneous-membrane-interaction-of-amphipathic-peptide-monomers-self-aggregates-and-cargo-complexes-detected-by-fluorescence-correlation-spectroscopy
#1
Luís Vasconcelos, Tõnis Lehto, Fatemeh Madani, Vlad Radoi, Mattias Hällbrink, Vladana Vukojević, Ülo Langel
Peptides able to translocate cell membranes while carrying macromolecular cargo, as cell-penetrating peptides (CPPs), can contribute to the field of drug delivery by enabling the transport of otherwise membrane impermeable molecules. Formation of non-covalent complexes between amphipathic peptides and oligonucleotides is driven by electrostatic and hydrophobic interactions. Here we investigate and quantify the coexistence of distinct molecular species in multiple equilibria, namely peptide monomer, peptide self-aggregates and peptide/oligonucleotide complexes...
September 26, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28893723/scientific-challenges-in-the-risk-assessment-of-food-contact-materials
#2
Jane Muncke, Thomas Backhaus, Birgit Geueke, Maricel V Maffini, Olwenn Viviane Martin, John Peterson Myers, Ana M Soto, Leonardo Trasande, Xenia Trier, Martin Scheringer
BACKGROUND: Food contact articles (FCAs) are manufactured from food contact materials (FCMs) that include plastics, paper, metal, glass, and printing inks. Chemicals can migrate from FCAs into food during storage, processing, and transportation. Food contact materials' safety is evaluated using chemical risk assessment (RA). Several challenges to the RA of FCAs exist. OBJECTIVES: We review regulatory requirements for RA of FCMs in the United States and Europe, identify gaps in RA, and highlight opportunities for improving the protection of public health...
September 11, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28740447/breast-dynamic-contrast-enhanced-mri-fibrocystic-changes-presenting-as-a-non-mass-enhancement-mimicking-malignancy
#3
Zorica C Milosevic, Mirjan M Nadrljanski, Zorka M Milovanovic, Nina Z Gusic, Slavko S Vucicevic, Olga S Radulovic
BACKGROUND: We aimed to analyse the morphokinetic features of breast fibrocystic changes (nonproliferative lesions, proliferative lesions without atypia and proliferative lesions with atypia) presenting as a non-mass enhancement (NME)in dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) examination. PATIENTS AND METHODS: Forty-six patients with histologically proven fibrocystic changes (FCCs) were retrospectively reviewed, according to Breast Imaging Reporting and Data System (BI-RADS) lexicon...
June 2017: Radiology and Oncology
https://www.readbyqxmd.com/read/28720130/psychosocial-and-behavioral-impact-of-breast-cancer-risk-assessed-by-testing-for-common-risk-variants-protocol-of-a-prospective-study
#4
Tatiane Yanes, Bettina Meiser, Mary-Anne Young, Rajneesh Kaur, Gillian Mitchell, Kristine Barlow-Stewart, Tony Roscioli, Jane Halliday, Paul James
BACKGROUND: The 'common variant, common disease' model predicts that a significant component of hereditary breast cancer unexplained by pathogenic variants in moderate or high-penetrance genes is due to the cumulative effect of common risk variants in DNA (polygenic risk). Assessing a woman's breast cancer risk by testing for common risk variants can provide useful information for women who would otherwise receive uninformative results by traditional monogenic testing. Despite increasing support for the utility of common risk variants in hereditary breast cancer, research findings have not yet been integrated into clinical practice...
July 18, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28436659/fluorescence-and-scattering-light-cross-correlation-spectroscopy-and-its-applications-in-homogeneous-immunoassay
#5
Jinjie Wang, Xiangyi Huang, Heng Liu, Chaoqing Dong, Jicun Ren
In this work, we propose fluorescence and scattering light cross-correlation spectroscopy (FSCCS) based on laser confocal configuration using silver nanoparticle (SNPs) and Alexa Fluor 488 (Alexa) as probe pairs. FSCCS is a single molecule (particle) method, and its principle is similar to that of fluorescence cross-correlation spectroscopy (FCCS). We established the setup of FSCCS using single wavelength laser and developed an immunoassay model of FSCCS. The reliability and adaptability of FSCCS method were evaluated by homogeneous sandwich immunoassay mode...
May 2, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28413227/david-c-drum-dc-fccs-c-an-accomplished-multi-faceted-individual
#6
Douglas M Brown
Pablo Picasso (1881-1973) is quoted as saying, "The meaning of life is to find your gift. The purpose of life is to give it away." With these few words Picasso captures the essence of David Drum's existence. From his birth in Moncton, NB, through his formal education in Toronto, ON, to his so-called "retirement" in Crystal Beach, ON, this paper spans 72 years, unveiling the broad range of opportunities for growth David Drum has encountered in his life's journey and documenting his acceptance of the obligations for proud philanthropy that accompany them...
March 2017: Journal of the Canadian Chiropractic Association
https://www.readbyqxmd.com/read/28332353/port-site-metastases-and-chimney-effect-of-b-ultrasound-guided-and-laparoscopically-assisted-hyperthermic-intraperitoneal-perfusion-chemotherapy
#7
Ming Chen Ba, Hui Long, Xiang Liang Zhang, Yuan Feng Gong, Zhao Fei Yan, Shuai Wang, Yun Qiang Tang, Shu Zhong Cui
PURPOSE: CO₂ leakage along the trocar (chimney effect) has been proposed to be an important factor underlying port-site metastasis after laparoscopic surgery. This study aimed to test this hypothesis by comparing the incidence of port-site metastasis between B-ultrasound-guided and laparoscopically-assisted hyperthermic intraperitoneal perfusion chemotherapy (HIPPC). MATERIALS AND METHODS: Sixty-two patients with malignant ascites induced by gastrointestinal or ovarian cancer were divided into two groups to receive either B-ultrasound-guided or laparoscopically-assisted HIPPC...
May 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/28192301/dynamic-interaction-between-actin-and-nesprin2-maintain-the-cell-nucleus-in-a-prestressed-state
#8
Abhishek Kumar, G V Shivashankar
Mechanical coupling between the nucleus and the cytoskeleton is indispensable for direct force transduction from the extra cellular matrix (ECM) to the chromatin. Although this physical coupling has been shown to be crucial for nuclear positioning and its function, the quantification of nuclear-cytoskeleton interaction has been lacking. In this paper, using various quantitative fluorescence spectroscopy techniques, we investigate the nature of this connection. High-resolution 3D imaging shows that nesprin2G forms short linear structures along actin stress fibers (ASFs) in the apical region of the nucleus...
November 11, 2016: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28025890/enhanced-microwave-absorption-performance-of-coated-carbon-nanotubes-by-optimizing-the-fe3o4-nanocoating-structure
#9
Na Li, Gui-Wen Huang, Yuan-Qing Li, Hong-Mei Xiao, Qing-Ping Feng, Ning Hu, Shao-Yun Fu
It is well accepted that the microwave absorption performance (MAP) of carbon nanotubes (CNTs) can be enhanced via coating magnetic nanoparticles on their surfaces. However, it is still unclear if the magnetic coating structure has a significant influence on the microwave absorption behavior. In this work, nano-Fe3O4 compact-coated CNTs (FCCs) and Fe3O4 loose-coated CNTs (FLCs) are prepared using a simple solvothermal method. The MAP of the Fe3O4-coated CNTs is shown to be adjustable via controlling the Fe3O4 nanocoating structure...
January 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28013230/evolution-of-plant-conducting-cells-perspectives-from-key-regulators-of-vascular-cell-differentiation
#10
REVIEW
Misato Ohtani, Nobuhiro Akiyoshi, Yuto Takenaka, Ryosuke Sano, Taku Demura
One crucial problem that plants faced during their evolution, particularly during the transition to growth on land, was how to transport water, nutrients, metabolites, and small signaling molecules within a large, multicellular body. As a solution to this problem, land plants developed specific tissues for conducting molecules, called water-conducting cells (WCCs) and food-conducting cells (FCCs). The well-developed WCCs and FCCs in extant plants are the tracheary elements and sieve elements, respectively, which are found in vascular plants...
January 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27974886/diagnostic-value-of-fecal-calprotectin-s100-a8-a9-test-in-children-with-chronic-abdominal-pain
#11
Stanisław Pieczarkowski, Kinga Kowalska-Duplaga, Przemko Kwinta, Przemysław Tomasik, Andrzej Wędrychowicz, Krzysztof Fyderek
Objectives. The aim of the study was to establish whether fecal calprotectin concentration (FCC) may be useful in children with chronic abdominal pain to differentiate between inflammatory bowel disease (IBD), other inflammatory gastrointestinal disorders, and functional gastrointestinal disorders. Methods. The study included 163 patients (median age 13 years), who were assigned to four study groups: group 0 (control), 22 healthy children; group 1, 33 children with functional gastrointestinal disorders; group 2, 71 children with inflammatory gastrointestinal disorders other than IBD; group 3, 37 children with IBD...
2016: Gastroenterology Research and Practice
https://www.readbyqxmd.com/read/27925474/fiber-development-and-matrix-production-in-tissue-engineered-menisci-using-bovine-mesenchymal-stem-cells-and-fibrochondrocytes
#12
Mary Clare McCorry, Lawrence J Bonassar
Mesenchymal stem cells (MSCs) have been investigated with promising results for meniscus healing and tissue engineering. While MSCs are known to contribute to extracellular matrix (ECM) production, less is known about how MSCs produce and align large organized fibers for application to tissue engineering the meniscus. The goal of this study was to investigate the capability of MSCs to produce and organize ECM molecules compared to meniscal fibrochondrocytes (FCCs). Bovine FCCs and MSCs were encapsulated in an anatomically accurate collagen meniscus using monoculture and co-culture of each cell type...
May 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/27851946/the-epidermal-growth-factor-receptor-forms-location-dependent-complexes-in-resting-cells
#13
Sibel Yavas, Radek Macháň, Thorsten Wohland
The epidermal growth factor receptor (EGFR) is a prototypical receptor tyrosine kinase involved in cell growth and proliferation and associated with various cancers. It is commonly assumed that after activation by binding of epidermal growth factor to the extracellular domain it dimerizes, followed by autophosphorylation of tyrosine residues at the intracellular domain. However, its oligomerization state before activation is controversial. In the absence of ligands, EGFR has been found in various, inconsistent amounts of monomeric, inactive dimeric, and oligomeric forms...
November 15, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27850058/418-integration-of-fccs-into-a-critical-care-education-program-at-an-oncologic-teaching-hospital
#14
Marian Von-Maszewski, Gregory Botz
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27618848/psychometric-properties-of-the-functional-capacity-card-sort-for-caregivers-of-people-with-dementia
#15
Catherine Verrier Piersol, E Adel Herge, Albert E Copolillo, Benjamin E Leiby, Laura N Gitlin
Caregiver appraisal influences care decisions and may place the person with dementia at risk. The Functional Capacity Card Sort (FCCS) was developed to determine caregiver appraisal. The objective of this study was to examine FCCS psychometric properties and utility, using data from a cross-sectional study (N = 86). The FCCS had moderately positive association (convergent validity) with a related activities of daily living (ADL) index (r = .43, p < .0001), weak negative association (discriminant validity) with an unrelated neuropsychiatric symptom index (r = -...
July 2016: OTJR: Occupation, Participation and Health
https://www.readbyqxmd.com/read/27493064/lipid-regulated-intramolecular-conformational-dynamics-of-snare-protein-ykt6
#16
Yawei Dai, Markus Seeger, Jingwei Weng, Song Song, Wenning Wang, Yan-Wen Tan
Cellular informational and metabolic processes are propagated with specific membrane fusions governed by soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNARE). SNARE protein Ykt6 is highly expressed in brain neurons and plays a critical role in the membrane-trafficking process. Studies suggested that Ykt6 undergoes a conformational change at the interface between its longin domain and the SNARE core. In this work, we study the conformational state distributions and dynamics of rat Ykt6 by means of single-molecule Förster Resonance Energy Transfer (smFRET) and Fluorescence Cross-Correlation Spectroscopy (FCCS)...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27374574/chlorophyll-catabolism-in-olive-fruits-var-arbequina-and-hojiblanca-during-maturation
#17
Honorio Vergara-Domínguez, José Julían Ríos, Beatriz Gandul-Rojas, María Roca
The central reaction of chlorophyll (chl) breakdown pathway occurring during olive fruits maturation is the cleavage of the macrocycle pheophorbide a to a primary fluorescent chl catabolite (pFCC) and it is catalyzed by two enzymes: pheophorbide a oxygenase (PaO) and red chl catabolite reductase (RCCR). In subsequent steps, pFCC is converted to different fluorescent chlorophyll catabolites (FCCs) and nonfluorescent chlorophyll catabolites (NCCs). This work demonstrated that RCCR activity of olive fruits is type II...
December 1, 2016: Food Chemistry
https://www.readbyqxmd.com/read/27302705/challenges-in-investigating-a-salmonellosis-outbreak-in-a-correctional-facility
#18
Anthony Olufemi Eshofonie, Huai Lin, Randy Paul Valcin, Darrel Irvin, Kathleen Goss, Jeremy Piper
Timely reporting of disease outbreak and access to the outbreak site are necessary for prompt investigation and control. Local health departments (LHDs) establish the reporting time frames, methods, and communication channels within their jurisdictions in advance. This report describes a salmonellosis outbreak investigation in a federal correctional center (FCC) that was delayed due to issues with jurisdictional ownership and with separate reporting protocols for the FCC. The setting of the outbreak within an FCC posed additional unique challenges to the outbreak investigation...
July 2016: Journal of Correctional Health Care
https://www.readbyqxmd.com/read/27300442/new-insight-into-metal-ion-driven-catalysis-of-nucleic-acids-by-influenza-pa-nter
#19
Daria Kotlarek, Remigiusz Worch
PA subunit of influenza RNA-dependent RNA polymerase deserves constantly increasing attention due to its essential role in influenza life cycle. N-terminal domain of PA (PA-Nter) harbors endonuclease activity, which is indispensable in viral transcription and replication. Interestingly, existing literature reports on in vitro ion preferences of the enzyme are contradictory. Some show PA-Nter activity exclusively with Mn2+, whereas others report Mg2+ as a natural cofactor. To clarify it, we performed a series of experiments with varied ion concentrations and substrate type...
2016: PloS One
https://www.readbyqxmd.com/read/27087436/development-and-reliability-of-the-functional-communication-classification-system-for-children-with-cerebral-palsy
#20
Elizabeth Barty, Katy Caynes, Leanne M Johnston
AIM: This paper describes the development, validation, and reliability of the Functional Communication Classification System (FCCS), designed to classify expressive communication skills of children with cerebral palsy (CP) aged 4 years and 5 years (between their fourth and sixth birthdays). METHOD: The Functional Communication Classification System (FCCS) was developed in 2006 using a literature review, client file audit, and expert consultative committee process in order to devise scale content, structure, and check clinical validity and utility...
October 2016: Developmental Medicine and Child Neurology
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