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Sibel Yavas, Radek Macháň, Thorsten Wohland
The epidermal growth factor receptor (EGFR) is a prototypical receptor tyrosine kinase involved in cell growth and proliferation and associated with various cancers. It is commonly assumed that after activation by binding of epidermal growth factor to the extracellular domain it dimerizes, followed by autophosphorylation of tyrosine residues at the intracellular domain. However, its oligomerization state before activation is controversial. In the absence of ligands, EGFR has been found in various, inconsistent amounts of monomeric, inactive dimeric, and oligomeric forms...
November 15, 2016: Biophysical Journal
Marian Von-Maszewski, Gregory Botz
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
Catherine Verrier Piersol, E Adel Herge, Albert E Copolillo, Benjamin E Leiby, Laura N Gitlin
Caregiver appraisal influences care decisions and may place the person with dementia at risk. The Functional Capacity Card Sort (FCCS) was developed to determine caregiver appraisal. The objective of this study was to examine FCCS psychometric properties and utility, using data from a cross-sectional study (N = 86). The FCCS had moderately positive association (convergent validity) with a related activities of daily living (ADL) index (r = .43, p < .0001), weak negative association (discriminant validity) with an unrelated neuropsychiatric symptom index (r = -...
July 2016: OTJR: Occupation, Participation and Health
Yawei Dai, Markus Seeger, Jingwei Weng, Song Song, Wenning Wang, Yan-Wen Tan
Cellular informational and metabolic processes are propagated with specific membrane fusions governed by soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNARE). SNARE protein Ykt6 is highly expressed in brain neurons and plays a critical role in the membrane-trafficking process. Studies suggested that Ykt6 undergoes a conformational change at the interface between its longin domain and the SNARE core. In this work, we study the conformational state distributions and dynamics of rat Ykt6 by means of single-molecule Förster Resonance Energy Transfer (smFRET) and Fluorescence Cross-Correlation Spectroscopy (FCCS)...
2016: Scientific Reports
Honorio Vergara-Domínguez, José Julían Ríos, Beatriz Gandul-Rojas, María Roca
The central reaction of chlorophyll (chl) breakdown pathway occurring during olive fruits maturation is the cleavage of the macrocycle pheophorbide a to a primary fluorescent chl catabolite (pFCC) and it is catalyzed by two enzymes: pheophorbide a oxygenase (PaO) and red chl catabolite reductase (RCCR). In subsequent steps, pFCC is converted to different fluorescent chlorophyll catabolites (FCCs) and nonfluorescent chlorophyll catabolites (NCCs). This work demonstrated that RCCR activity of olive fruits is type II...
December 1, 2016: Food Chemistry
Anthony Olufemi Eshofonie, Huai Lin, Randy Paul Valcin, Darrel Irvin, Kathleen Goss, Jeremy Piper
Timely reporting of disease outbreak and access to the outbreak site are necessary for prompt investigation and control. Local health departments (LHDs) establish the reporting time frames, methods, and communication channels within their jurisdictions in advance. This report describes a salmonellosis outbreak investigation in a federal correctional center (FCC) that was delayed due to issues with jurisdictional ownership and with separate reporting protocols for the FCC. The setting of the outbreak within an FCC posed additional unique challenges to the outbreak investigation...
July 2016: Journal of Correctional Health Care
Daria Kotlarek, Remigiusz Worch
PA subunit of influenza RNA-dependent RNA polymerase deserves constantly increasing attention due to its essential role in influenza life cycle. N-terminal domain of PA (PA-Nter) harbors endonuclease activity, which is indispensable in viral transcription and replication. Interestingly, existing literature reports on in vitro ion preferences of the enzyme are contradictory. Some show PA-Nter activity exclusively with Mn2+, whereas others report Mg2+ as a natural cofactor. To clarify it, we performed a series of experiments with varied ion concentrations and substrate type...
2016: PloS One
Elizabeth Barty, Katy Caynes, Leanne M Johnston
AIM: This paper describes the development, validation, and reliability of the Functional Communication Classification System (FCCS), designed to classify expressive communication skills of children with cerebral palsy (CP) aged 4 years and 5 years (between their fourth and sixth birthdays). METHOD: The Functional Communication Classification System (FCCS) was developed in 2006 using a literature review, client file audit, and expert consultative committee process in order to devise scale content, structure, and check clinical validity and utility...
October 2016: Developmental Medicine and Child Neurology
Sherri L LaVela, Stephanie Turcios, Alex Malhiot, Bella Etingen, Jennifer N Hill, Scott Miskevics
INTRODUCTION: Family-centered care (FCC) involves partnering with patients and family/caregivers ("caregivers"), engaging them in decisions, and providing support. FCC may be measured across 3 dimensions: respect, collaboration, and support and needs likely vary by caregiver age. The objective was to examine and compare caregiver perceptions of FCC by age. METHOD: We mailed surveys to family/caregivers of Veterans. We collected demographics and measured respect, collaboration, and support using the Family-Centered Care Survey (FCCS)...
June 2016: Families, Systems & Health: the Journal of Collaborative Family Healthcare
Mary Clare McCorry, Jennifer L Puetzer, Lawrence J Bonassar
BACKGROUND: Bone marrow mesenchymal stem cells (MSCs) have shown positive therapeutic effects for meniscus regeneration and repair. Preliminary in vitro work has indicated positive results for MSC applications for meniscus tissue engineering; however, more information is needed on how to direct MSC behavior. The objective of this study was to examine the effect of MSC co-culture with primary meniscal fibrochondrocytes (FCCs) in a three-dimensional collagen scaffold in fibrochondrogenic media...
2016: Stem Cell Research & Therapy
Thomas Antoine, David Ott, Katharina Ebell, Kerrin Hansen, Luc Henry, Frank Becker, Stefan Hannus
G protein-coupled receptors (GPCRs) mediate many important physiological functions and are considered as one of the most successful therapeutic target classes for a wide spectrum of diseases. Drug discovery projects generally benefit from a broad range of experimental approaches for screening compound libraries and for the characterization of binding modes of drug candidates. Owing to the difficulties in solubilizing and purifying GPCRs, assay formats have been so far mainly limited to cell-based functional assays and radioligand binding assays...
June 1, 2016: Analytical Biochemistry
Isabela Costa Guimarães, Kelen Cristina dos Reis, Evandro Galvão Tavares Menezes, Paulo Rogério Siriano Borges, Ariel Costa Rodrigues, Renato Leal, Thais Hernandes, Elisângela Helena Nunes de Carvalho, Eduardo Valério de Barros Vilas Boas
This work evaluates fresh-cut carrots (FCC) coated with montmorillonite (MMT) subjected to passive modified atmosphere packaging. Carrots were sanitized, cooled, peeled and sliced. Half of the FCC were coated with MMT nanoparticle film and the other half were not. All FCCs were packed in a polypropylene rigid tray, covered with a polypropylene rigid lid or sealed with polyethylene + propylene film, in four treatments (RL, rigid lid; RLC, rigid lid + coating; ST, sealed tray; STC, sealed tray + coating)...
2016: International Journal of Food Sciences and Nutrition
Wen-Ling Wang, Jui-Ying Feng, Chi-Jen Wang, Jing-Huei Chen
AIMS: This study aimed to develop a family-centered care survey for Chinese adult intensive care units and to establish the survey's psychometric properties. BACKGROUND: Family-centered care (FCC) is widely recognized as an ideal model of care. Few studies have explored FCC perceptions among family members of adult critical care patients in Asian countries, and no Chinese FCC measurement has been developed. METHODS: An English version of the 3-factor family-centered care survey for adult intensive care units (FCCS-AICU) was translated into Chinese using a modified back translation procedure...
February 2016: Applied Nursing Research: ANR
Hiroki Uehara, Takuya Tamura, Hideyuki Yamashita, Takeshi Yamanobe, Hiroyasu Masunaga
Ultrahigh molecular weight polyethylene (UHMW-PE) membranes were prepared using biaxial melt-drawing and subsequent melt-shrinking. Electron microscopy observations indicate that the former membrane has more extended-chain crystals (ECCs), whereas the latter is mainly composed of folded-chain crystals (FCCs). Corresponding double-melting endotherms are recorded on differential scanning calorimetry (DSC) measurements. Detailed assignments of such double-melting components are performed using in situ X-ray measurements during heating...
December 31, 2015: Journal of Physical Chemistry. B
Gustavo Ferrer, Fanny Tse, Jose Ramirez, Roxana Karimzadeh, Tara Rowland, Marlow Hernandez
No abstract text is available yet for this article.
December 2015: Critical Care Medicine
Christine Fauth, Katharina Steindl, Annick Toutain, Sandra Farrell, Martina Witsch-Baumgartner, Daniela Karall, Pascal Joset, Sebastian Böhm, Alessandra Baumer, Oliver Maier, Johannes Zschocke, Rosanna Weksberg, Christian R Marshall, Anita Rauch
Hypomorphic germline mutations in the PIGA (phosphatidylinositol glycan class A) gene recently were recognized as the cause of a clinically heterogeneous spectrum of X-linked disorders including (i) early onset epileptic encephalopathy with severe muscular hypotonia, dysmorphism, multiple congenital anomalies, and early death ("MCAHS2"), (ii) neurodegenerative encephalopathy with systemic iron overload (ferro-cerebro-cutaneous syndrome, "FCCS"), and (iii) intellectual disability and seizures without dysmorphism...
February 2016: American Journal of Medical Genetics. Part A
Jan W Krieger, Anand P Singh, Nirmalya Bag, Christoph S Garbe, Timothy E Saunders, Jörg Langowski, Thorsten Wohland
Single-plane illumination (SPIM) or total internal reflection fluorescence (TIRF) microscopes can be combined with fast and single-molecule-sensitive cameras to allow spatially resolved fluorescence (cross-) correlation spectroscopy (FCS or FCCS, hereafter referred to FCS/FCCS). This creates a powerful quantitative bioimaging tool that can generate spatially resolved mobility and interaction maps with hundreds to thousands of pixels per sample. These massively parallel imaging schemes also cause less photodamage than conventional single-point confocal microscopy-based FCS/FCCS...
December 2015: Nature Protocols
Morgan Marita, Yuxiao Wang, Megan J Kaliszewski, Kevin C Skinner, William D Comar, Xiaojun Shi, Pranathi Dasari, Xuewu Zhang, Adam W Smith
Plexins are single-pass transmembrane receptors that bind the axon guidance molecules semaphorins. Single-pass transmembrane proteins are an important class of receptors that display a wide variety of activation mechanisms, often involving ligand-dependent dimerization or conformational changes. Resolving the activation mechanism and dimerization state of these receptors is extremely challenging, especially in a live-cell environment. Here, we report on the dimerization state of PlexinA4 and its response to activation by semaphorin binding...
November 3, 2015: Biophysical Journal
Jaime A García-Inzunza, Ana M Valles-Medina, Fátima A Muñoz, Guadalupe Delgadillo-Ramos, Lidia G Compean-Ortiz
OBJECTIVE: To 1) translate / transculturally adapt the original (English-language) combined Foot Care Confidence Scale / Foot-Care Behavior instrument (FCCS-FCB) to produce a Mexican-Spanish version and 2) determine its validity and reliability in a population with diabetes in Tijuana, Mexico. METHODS: The original FCCS-FCB was translated (and back-translated), the content validated (by a group of health professional experts), and the instrument applied to 304 patients 23-78 years old in diabetes support groups in Tijuana, Mexico...
July 2015: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
Stefan Wennmalm, Volodymyr Chmyrov, Jerker Widengren, Lars Tjernberg
Oligomers formed by the amyloid β-peptide (Aβ) are pathogens in Alzheimer's disease. Increased knowledge on the oligomerization process is crucial for understanding the disease and for finding treatments. Ideally, Aβ oligomerization should be studied in solution and at physiologically relevant concentrations, but most popular techniques of today are not capable of such analyses. We demonstrate here that the combination of Förster Resonance Energy Transfer and Fluorescence Correlation Spectroscopy (FRET-FCS) has a unique ability to detect small subpopulations of FRET-active molecules and oligomers...
December 1, 2015: Analytical Chemistry
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