Farzad Izak Shirian, Milad Karimi, Maryam Alipour, Siamak Salami, Mitra Nourbakhsh, Samira Nekufar, Nahid Safari-Alighiarloo, Masoumeh Tavakoli-Yaraki
BACKGROUND: Metabolic plasticity gives cancer cells the ability to shift between signaling pathways to facilitate their growth and survival. This study investigates the role of glucose deprivation in the presence and absence of beta-hydroxybutyrate (BHB) in growth, death, oxidative stress and the stemness features of lung cancer cells. METHODS AND RESULTS: A549 cells were exposed to various glucose conditions, both with and without beta-hydroxybutyrate (BHB), to evaluate their effects on apoptosis, mitochondrial membrane potential, reactive oxygen species (ROS) levels using flow cytometry, and the expression of CD133, CD44, SOX-9, and β-Catenin through Quantitative PCR...
April 24, 2024: Molecular Biology Reports