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life cycle testing

Yingliang Ou, Jiafa He, Yubin Liu
The miR-490-3p was transfected into HepG2 cells to explore the correlation between miR-490-3p and hepatocellular carcinoma cell proliferation, apoptosis, and autophagy and its downstream target gene ATG7. Then we could possibly provide a mechanism for the treatment of hepatocellular carcinoma. MiR-490-3p was screened out by fold change > 4 and P < 0.01 using gene microarray data. The expression level of miR-490-3p was tested by qRT-PCR and the prognosis analysis was achieved by using TCGA data...
April 20, 2018: IUBMB Life
Lauren E Lees, Stacy A Krueger-Hadfield, Andrew J Clark, Elizabeth A Duermit, Erik E Sotka, Courtney J Murren
Theory predicts that the maintenance of haplodiplontic life cycles requires ecological differences between the haploid gametophytes and diploid sporophytes, yet evidence of such differences remain scarce. The haplodiplontic red seaweed Gracilaria vermiculophylla has invaded the temperate estuaries of the Northern Hemisphere, where it commonly modifies detrital and trophic pathways. In native populations, abundant hard substratum enables spore settlement, and gametophyte:tetrasporophyte ratios are approximately 40:60...
April 20, 2018: Journal of Phycology
Jack A Rall
This is a story about a great scientist, luck, great discovery that changed the future direction of muscle research, war, a clandestine war mission, postwar politics, and an attempt to rewrite scientific history. Albert Szent-Gyorgyi, at 44 yr of age, won the Nobel Prize in 1937 for his work on vitamin C and the establishment of the groundwork of the citric acid cycle. He now wanted to investigate one of the fundamental aspects of life and settled on the study of muscle contraction. The Szent-Gyorgyi laboratory in Hungary during World War II demonstrated that contraction could be reproduced in vitro by threads consisting of just two proteins, myosin and the newly discovered protein by Bruno Straub that they called actin...
June 1, 2018: Advances in Physiology Education
Lorenzo Carbone, Paolo Tomislav Moro, Mallory Gobet, Stephen Munoz, Matthew Devany, Steven G Greenbaum, Jusef Hassoun
The lithium oxygen battery has a theoretical energy density potentially meeting the challenging requirements of electric vehicles. However, safety concerns and short lifespan hinder its application in practical systems. In this work we show a cell configuration, including a multi-walled carbon nanotubes electrode (MWCNTs) and a lowly flammable glyme electrolyte, capable of hundreds of cycles without signs of decay. Nuclear magnetic resonance and electrochemical tests confirm the suitability of the electrolyte in a practical battery, while morphological and structural aspects revealed by electron microscopy and X-ray diffraction demonstrate the reversible formation and dissolution of lithium peroxide during the electrochemical process...
April 20, 2018: ACS Applied Materials & Interfaces
Joyce O'Shaughnessy, Angela DeMichele, Cynthia X Ma, Paul Richards, Denise A Yardley, Gail Shaw Wright, Kevin Kalinsky, Ronald Steis, Sami Diab, Gerard Kennealey, Ryan Geschwindt, Wei Jiang, Hope S Rugo
PURPOSE: The Janus-associated kinase (JAK)/signal transducer and activator of transcription pathway is a key regulator of inflammatory signaling, associated with tumorigenesis, cell survival, and progression. This randomized phase 2 trial evaluated the efficacy and safety of the addition of ruxolitinib, a JAK1/JAK2 inhibitor, to capecitabine in patients with HER2-negative advanced breast cancer and high systemic inflammation (modified Glasgow Prognostic Score [mGPS] ≥ 1). METHODS: Patients with ≤ 2 prior chemotherapy regimens for advanced or metastatic disease or hormone receptor-positive patients with disease progression on prior hormonal therapies were randomized 1:1 to 21-day cycles of ruxolitinib (n = 76) or placebo (n = 73) plus capecitabine...
April 19, 2018: Breast Cancer Research and Treatment
Li Zhao, Bo He, Kai-Rui Li, Fei Li, Lu-Yao Zhang, Xian-Qiang Li, Yong-Hong Liu
BACKGROUND: Melophagus ovinus (sheep ked) is a blood-feeding ectoparasite that belongs to the family Hippoboscidae (Diptera: Hippoboscoidea) and mainly parasitizes sheep. The life-cycle of M. ovinus consists of three stages: larva, pupa and adult. It has a worldwide distribution and has been found in four provinces of China, especially South Xinjiang. In addition to causing direct damage to animal hosts, M. ovinus serves as a vector for disease transmission. In this study, our aim was to investigate the presence of Anaplasma spp...
April 19, 2018: Parasites & Vectors
Ming-Cheng Chan, Elise Chia-Hui Tan, Ming-Chin Yang
Objective: The aim of this study was to evaluate the cost-effectiveness of the long-acting beta-2 agonist (LABA)/long-acting muscarinic antagonist (LAMA) dual bronchodilator indacaterol/glycopyrronium (IND/GLY) as a maintenance treatment for COPD patients from the perspective of health care payer in Taiwan. Patients and methods: We adopted a patient-level simulation model, which included a cohort of COPD patients aged ≥40 years. The intervention used in the study was the treatment using IND/GLY, and comparators were tiotropium or salmeterol/fluticasone combination (SFC)...
2018: International Journal of Chronic Obstructive Pulmonary Disease
Ewa Mikulak, Aleksandra Gliniewicz, Marta Przygodzka, Jolanta Solecka
Comparative of studies of genomes of invertebrates and humans shows that in invertebrates including insects there are numerous homologues of human’s genes coding proteins involved in recognition pathogens or transduction of the expression signal. Thanks this features, insects such as Drosophila melanogaster M., Blattella germanica L., Culex quinquefasciatus S., Bombyx mori L. and Galleria mellonella L. are used in studies on virulence, host resistance or in assessing the in vivo efficacy of antibiotics, fungicides and other biologically active substances...
2018: Przegla̧d Epidemiologiczny
Andrea Corona, Morten Ambye-Jensen, Giovanna Croxatto Vega, Michael Zwicky Hauschild, Morten Birkved
The Green biorefinery (GBR) is a biorefinery concept that converts fresh biomass into value-added products. The present study combines a Process Flowsheet Simulation (PFS) and Life Cycle Assessment (LCA) to evaluate the technical and environmental performance of different GBR configurations and the cascading utilization of the GBR output. The GBR configurations considered in this study, test alternatives in the three main steps of green-biorefining: fractionation, precipitation, and protein separation. The different cascade utilization alternatives analyse different options for press-pulp utilization, and the LCA results show that the environmental profile of the GBR is highly affected by the utilization of the press-pulp and thus by the choice of conventional product replaced by the press-pulp...
April 13, 2018: Science of the Total Environment
Steven D Melvin, Frederic D L Leusch, Anthony R Carroll
Amphibians use wetlands in urban and agricultural landscapes for breeding, growth and development. Fungicides and other pesticides used in these areas have therefore been identified as potential threats that could contribute towards amphibian population declines. However, relatively little is known about how such chemicals influence sensitive early life-stages or how short episodic exposures influence sub-lethal physiological and metabolic pathways. The present study applied untargeted metabolomics to evaluate effects in early post-hatch amphibian larvae exposed to the anti-androgenic fungicides vinclozolin and propiconazole...
April 11, 2018: Aquatic Toxicology
Naylene C S Silva, Aldilene S Lima, Carolina R Silva, Danilo R B Brito, José A A Cutrim-Junior, Marlise N Milhomem, Livio M Costa-Junior
Haemonchus contortus is the most prevalent nematode in tropical and subtropical regions and is responsible for significant losses in small ruminant production systems. Control of this parasite is based on the use of synthetic anthelmintics; although effective, prolonged and indiscriminate use can select for populations of resistant nematodes. Hydrolyzed yeast can help prevent populations of nematodes and is low in cost. The present study aimed to evaluate the preventive effect of hydrolyzed yeast on H. contortus through in vitro and in vivo assays...
April 30, 2018: Veterinary Parasitology
Oscar Araque, Nelson Arzola, Edgar Hernández
This research deals with the fatigue behavior of butt-welded joints, by considering the geometry and post-welding cooling cycles, as a result of cooling in quiet air and immersed in water. ASTM A-36 HR structural steel was used as the base metal for the shielded metal arc welding (SMAW) process with welding electrode E6013. The welding reinforcement was 1 mm and 3 mm, respectively; axial fatigue tests were carried out to determine the life and behavior in cracks propagation of the tested welded joints, mechanical characterization tests of properties in welded joints such as microhardness, Charpy impact test and metallographic analysis were carried out...
April 12, 2018: Materials
Lindsay A Jibb, Bonnie J Stevens, Paul C Nathan, Emily Seto, Joseph A Cafazzo, Donna L Johnston, Vanessa Hum, Jennifer N Stinson
BACKGROUND: Pain in adolescents with cancer is common and negatively impacts health-related quality of life. The Pain Squad+ smartphone app, capable of providing adolescents with real-time pain management support, was developed to enhance pain management using a phased approach (ie, systematic review, consensus conference and vetting, iterative usability testing cycles). A 28-day Pain Squad+ pilot was conducted with 40 adolescents with cancer to evaluate the feasibility of implementing the app in a future clinical trial and to obtain estimates of treatment effect...
April 6, 2018: JMIR MHealth and UHealth
Chao Cui, Mingqiang Li, Xiaoliang Zhang
Rechargeable Ni-Zn batteries, with high safety, low cost and nontoxicity, can be expected to compete with lithium-ion batteries for market share. However, the issue of dissolution of zinc electrode largely limit the battery cycle life and remains unsolved. We designed a kind of graphene-ZnO hybrid electrode in which in-situ cutting of graphene into short nanoribbons can effectively anchor plenty of zinc atoms onto the surface of graphene. This not only thoroughly fixes the issue of dissolution of zinc electrode but also increases the specific surface areas of zinc and promotes chemical reaction rate of the charge-discharge processes...
April 4, 2018: Scientific Reports
Joel Ward, Natasha Lewis, Dimitris A Tsitsikas
Hydroxyurea is the gold standard treatment for prevention of vaso-occlusive crises in patients with sickle-cell anaemia. It has a narrow therapeutic index and dangerous side effects including cytopenias. There is high variation in dose-response across the population. Therefore, a robust outpatient monitoring programme is crucial to ensure efficacy and safety of treatment. However, there has historically been difficulty engaging the target population in regular laboratory test monitoring programmes. This project aimed to ensure that all patients on hydroxyurea had routine blood tests at least once every 2 months which were reviewed and acted upon within the 3-year project life cycle...
2018: BMJ Open Quality
Hannah M L Young, Daniel S March, Matthew P M Graham-Brown, Arwel W Jones, Ffion Curtis, Charlotte S Grantham, Darren R Churchward, Patrick Highton, Alice C Smith, Sally J Singh, Chris Bridle, James O Burton
Background: Intradialytic cycling (IDC), delivered during haemodialysis (HD), has the potential to improve many health issues. This systematic review and meta-analysis examine the evidence on the effects of IDC on exercise capacity, quality of life (QoL), physical function and cardiovascular health. Methods: Twenty-four databases were searched alongside Internet and hand searching, and consultation with experts. Eligibility criteria were cluster randomized, randomized and quasi-randomized controlled trials (RCTs) of IDC versus usual care in prevalent adult HD patients...
March 28, 2018: Nephrology, Dialysis, Transplantation
Vahid Karimi, Arash Ghalyanchilangeroudi, Masoud Hashemzadeh, Forough Rahimi, Mohammad Taha Zabihi Petroudi, Reza Kh Farahani, Hossein Maghsoudloo, Hamed Abdollahi
Infectious bronchitis (IB) is a highly infectious avian pathogen, which affects the respiratory tract, gut, reproductive system, and kidney of chicks of all ages. Many different serotypes of IB virus (IBV) are recognized which cause different clinical manifestations. According to the antigenic differences, different serotypes of the virus do not cross-protect. Massachusetts serotype induces the best cross-protection against other serotypes. Recently, the IBV QX strain has been detected in Iran. QX strain causes permanent damage to the oviduct if it occurs in the early life cycle and is a significant factor in layer and breeder chicken flocks...
March 2018: Virusdisease
Prajakta Pradhan, Marie L Nguyen
Herpes Simplex Virus (HSV) is the cause of cold sores, blindness and encephalitis and often leads to recurrent infections. Use of current anti-viral therapies can be limited when drug resistant HSV mutants arise. Thus, novel drugs for the treatment of HSV are needed. Previous research in our laboratory has determined that the telomerase inhibitor, MST-312, interferes with multiple steps of the HSV life cycle. The structure of MST-312 contains moieties related to a natural compound found in green tea, epigallocatechin gallate (EGCG)...
March 28, 2018: Virus Research
Ping Hou, Jiarui Cai, Shen Qu, Ming Xu
In life cycle assessment (LCA), collecting unit process data from the empirical sources (i.e., meter readings, operation logs/journals) is often costly and time-consuming. We propose a new computational approach to estimate missing unit process data solely relying on limited known data based on a similarity-based link prediction method. The intuition is that similar processes in a unit process network tend to have similar material/energy inputs and waste/emission outputs. We use the ecoinvent 3.1 unit process datasets to test our method in four steps: 1) dividing the datasets into a training set and a test set; 2) randomly removing certain numbers of data in the test set indicated as missing; 3) using similarity-weighted means of various numbers of most similar processes in the training set to estimate the missing data in the test set; and 4) comparing estimated data with the original values to determine the performance of the estimation...
March 30, 2018: Environmental Science & Technology
Lander Foquet, Carola Schafer, Nana K Minkah, Daniel G W Alanine, Erika L Flannery, Ryan W J Steel, Brandon K Sack, Nelly Camargo, Matthew Fishbaugher, Will Betz, Thao Nguyen, Zachary P Billman, Elizabeth M Wilson, John Bial, Sean C Murphy, Simon J Draper, Sebastian A Mikolajczak, Stefan H I Kappe
The invention of liver-humanized mouse models has made it possible to directly study the preerythrocytic stages of Plasmodium falciparum . In contrast, the current models to directly study blood stage infection in vivo are extremely limited. Humanization of the mouse blood stream is achievable by frequent injections of human red blood cells (hRBCs) and is currently the only system with which to study human malaria blood stage infections in a small animal model. Infections have been primarily achieved by direct injection of P...
2018: Frontiers in Immunology
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