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Treg, immunology

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https://www.readbyqxmd.com/read/29676458/an-optimized-method-to-measure-human-foxp3-regulatory-t-cells-from-multiple-tissue-types-using-mass-cytometry
#1
Nicholas A J Dawson, Avery J Lam, Laura Cook, Romy E Hoeppli, Raewyn Broady, Anne M Pesenacker, Megan K Leving
Mass cytometry has facilitated the discovery of complex immunological networks and immune cell interactions; however, there are limited reports of using this technology to measure expression of intranuclear antigens such as transcription factors. Herein we describe testing of multiple staining methods to detect FOXP3, the lineage-defining transcription factor for regulatory T cells (Tregs), by mass cytometry. A custom paraformaldehyde/saponin-based fixation and permeabilization method was found to be the optimal method for mass cytometry-based Treg detection, yielding the best balance of FOXP3 detection and preserved cell surface marker expression...
April 20, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/29664967/role-of-il-23-signaling-in-the-progression-of-premalignant-oral-lesions-to-cancer
#2
Blaine Caughron, Yi Yang, M Rita I Young
Mice bearing carcinogen-induced premalignant oral lesions were previously shown to have a pro-inflammatory phenotype, which is replaced with an immune inhibitory phenotype as lesions progress to cancer. Since Th17 cells are prominent at the premalignant lesion state and their levels are supported by IL-23, studies used mice that were IL-23 receptor deficient (IL-23R KO) to determine the requirement for IL-23 signaling in the immunological and clinical status of mice with premalignant oral lesions. The results showed a dependence on IL-23 signaling for the pro-inflammatory state of mice with oral lesions as levels of IL-2, IFN-γ, IL-6, IL-17 and TNF-α were elevated in wildtype mice with premalignant oral lesions, but not in IL-23R KO mice...
2018: PloS One
https://www.readbyqxmd.com/read/29664397/computer-guided-design-of-optimal-microbial-consortia-for-immune-system-modulation
#3
Richard R Stein, Takeshi Tanoue, Rose L Szabady, Shakti K Bhattarai, Bernat Olle, Jason M Norman, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Georg K Gerber, Chris Sander, Kenya Honda, Vanni Bucci
Manipulation of the gut microbiota holds great promise for the treatment of diseases. However, a major challenge is the identification of therapeutically potent microbial consortia that colonize the host effectively while maximizing immunologic outcome. Here, we propose a novel workflow to select optimal immune-inducing consortia from microbiome composition and immune effectors measurements. Using published and newly generated microbial and regulatory T-cell (Treg ) data from germ-free mice, we estimate the contribution of twelve Clostridia strains with known immune-modulating effect to Treg induction...
April 17, 2018: ELife
https://www.readbyqxmd.com/read/29662489/immunological-approaches-towards-cancer-and-inflammation-a-cross-talk
#4
REVIEW
Xinglong Qu, Ying Tang, Shucheng Hua
The inflammation is the protective response of the body against various harmful stimuli; however, the aberrant and inappropriate activation tends to become harmful. The acute inflammatory response tends to resolved once the offending agent is subside but this acute response becomes chronic in nature when the body is unable to successfully neutralized the noxious stimuli. This chronic inflammatory microenvironment is associated with the release of various pro-inflammatory and oncogenic mediators such as nitric oxide (NO), cytokines [IL-1β, IL-2, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α)], growth factor, and chemokines...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29656117/baicalin-modulates-the-treg-teff-balance-to-alleviate-uveitis-by-activating-the-aryl-hydrocarbon-receptor
#5
Wenjie Zhu, Xiaoqing Chen, Jianfeng Yu, Yichen Xiao, Yingqi Li, Shangtao Wan, Wenru Su, Dan Liang
Autoimmune uveitis is a sight-threatening ocular inflammatory disorder. Immunological inflammation is regarded as the key to pathogenesis in autoimmune uveitis. Baicalin, the major bioactive component of Scutellaria baicalensis, possesses immunomodulatory properties. However, the role of baicalin in uveitis and its underlying mechanisms remain unclear. In the current study, we found that baicalin treatment obviously inhibited the intraocular inflammatory process in mice with experimental autoimmune uveitis, along with clear declines in infiltrated inflammatory cells and inflammatory cytokine transcription in the retina and draining lymph nodes...
April 12, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29618596/alternative-splicing-of-foxp3-controls-regulatory-t-cell-effector-functions-and-is-associated-with-human-atherosclerotic-plaque-stability
#6
Anne-Laure Joly, Christina Seitz, Sang Liu, Nikolai V Kouznetsov, Karl Gertow, Lisa S Westerberg, Gabrielle Paulsson-Berne, Göran K Hansson, John Andersson
<u>Rationale:</u> Regulatory T (Treg) cells suppress immune responses and have been shown to attenuate atherosclerosis. The Treg cell lineage specification factor FOXP3 is essential for Treg cells' ability to uphold immunological tolerance. In humans, FOXP3 exists in several different isoforms, however, their specific role is poorly understood. <u>Objective:</u> To define the regulation and functions of the two major FOXP3 isoforms, FOXP3fl and FOXP3Δ2, as well as to establish whether their expression is associated with ischemic atherosclerotic disease...
April 4, 2018: Circulation Research
https://www.readbyqxmd.com/read/29616680/comparison-of-t-helper-cell-patterns-in-primary-open-angle-glaucoma-and-normal-pressure-glaucoma
#7
Chunyu Guo, Ningbo Wu, Xiaoyin Niu, Yue Wu, Dongfeng Chen, Wenyi Guo
BACKGROUND HSP60-related immunological activities are found in normal-pressure glaucoma (NPG) patients, in whom an elevated intraocular pressure (IOP) found in primary open-angle glaucoma (POAG) is not observed. HSP60 was found in POAG and NPG patients, while anti-HSP60 level was mainly found to be higher in NPG patients. The purpose of this study was to compare the percentages of Th cells and levels of related cytokines, attempting to provide evidence to explain this discrepancy. MATERIAL AND METHODS Blood samples from POAG, NPG, and normal control (NC) groups were collected and peripheral blood monocytes were isolated and cultured with or without the stimulation of HSP60...
April 4, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29595533/ido1-expression-is-associated-with-immune-tolerance-and-poor-prognosis-in-patients-with-surgically-resected-esophageal-cancer
#8
Yuki Kiyozumi, Yoshifumi Baba, Kazuo Okadome, Taisuke Yagi, Takatsugu Ishimoto, Masaaki Iwatsuki, Yuji Miyamoto, Naoya Yoshida, Masayuki Watanabe, Yoshihiro Komohara, Hideo Baba
OBJECTIVES: To evaluate the relationship between indoleamine 2, 3-dioxygenase (IDO1) expression and tumoral immune status and clinical outcome in esophageal cancer. SUMMARY BACKGROUND DATA: IDO1 is a primary enzyme that generates immunosuppressive metabolites such as tryptophan and kynurenine. Like the PD-1/PD-L1 pathway, IDO1 plays a major role in tumor immunology and is a potential immune-based therapeutic target. METHODS: The expressions of IDO1, CD8 (a marker of cytotoxic T cells), FOXP3 [a marker of regulatory T cells (Treg)], and PD-L1 in 305 curatively resected esophageal cancers were evaluated by immunostaining...
March 27, 2018: Annals of Surgery
https://www.readbyqxmd.com/read/29594822/sicilian-centenarian-offspring-are-more-resistant-to-immune-ageing
#9
Graziella Rubino, Matteo Bulati, Anna Aiello, Stefano Aprile, Caterina Maria Gambino, Francesco Gervasi, Calogero Caruso, Giulia Accardi
BACKGROUND: Immunosenescence constitutes a major indirect cause of morbidity and mortality in the elderly. Previous analysis of immune signatures in a cohort of centenarian offspring showed an intermediate immunophenotype between age-matched and younger controls. AIMS: To confirm and extend the previous studies performing further phenotypical analysis in centenarian offspring and controls. METHODS: Analysis of Treg cells, γδ T cells, mucosal-associated invariant T cells, and senescent immune T cells was performed in centenarian offspring and controls...
March 28, 2018: Aging Clinical and Experimental Research
https://www.readbyqxmd.com/read/29593715/comparative-analysis-of-the-regulatory-t-cells-dynamics-in-peripheral-blood-in-human-and-porcine-polytrauma
#10
Rafael Serve, Ramona Sturm, Lukas Schimunek, Philipp Störmann, David Heftrig, Michel P J Teuben, Elsie Oppermann, Klemens Horst, Roman Pfeifer, Tim P Simon, Yannik Kalbas, Hans-Christoph Pape, Frank Hildebrand, Ingo Marzi, Borna Relja
Background: Severely injured patients experience substantial immunological stress in the aftermath of traumatic insult, which often results in systemic immune dysregulation. Regulatory T cells (Treg) play a key role in the suppression of the immune response and in the maintenance of immunological homeostasis. Little is known about their presence and dynamics in blood after trauma, and nothing is known about Treg in the porcine polytrauma model. Here, we assessed different subsets of Treg in trauma patients (TP) and compared those to either healthy volunteers (HV) or data from porcine polytrauma...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29593708/mucosal-delivery-of-fusion-proteins-with-bacillus-subtilis-spores-enhances-protection-against-tuberculosis-by-bacillus-calmette-gu%C3%A3-rin
#11
Alastair Copland, Gil R Diogo, Peter Hart, Shane Harris, Andy C Tran, Mathew J Paul, Mahavir Singh, Simon M Cutting, Rajko Reljic
Tuberculosis (TB) is the most deadly infectious disease in existence, and the only available vaccine, Bacillus Calmette-Guérin (BCG), is almost a century old and poorly protective. The immunological complexity of TB, coupled with rising resistance to antimicrobial therapies, necessitates a pipeline of diverse novel vaccines. Here, we show that Bacillus subtilis spores can be coated with a fusion protein 1 ("FP1") consisting of Mycobacterium tuberculosis (Mtb) antigens Ag85B, ACR, and HBHA. The resultant vaccine, Spore-FP1, was tested in a murine low-dose Mtb aerosol challenge model...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29578533/microrna-663-induces-immune-dysregulation-by-inhibiting-tgf-%C3%AE-1-production-in-bone-marrow-derived-mesenchymal-stem-cells-in-patients-with-systemic-lupus-erythematosus
#12
Linyu Geng, Xiaojun Tang, Kangxing Zhou, Dandan Wang, Shiying Wang, Genhong Yao, Weiwei Chen, Xiang Gao, Wanjun Chen, Songtao Shi, Nan Shen, Xuebing Feng, Lingyun Sun
Mesenchymal stem cells (MSCs) are critical for immune regulation. Although several microRNAs (miRNAs) have been shown to participate in autoimmune pathogenesis by affecting lymphocyte development and function, the roles of miRNAs in MSC dysfunction in autoimmune diseases remain unclear. Here, we show that patients with systemic lupus erythematosus (SLE) display a unique miRNA signature in bone marrow-derived MSCs (BMSCs) compared with normal controls, among which miR-663 is closely associated with SLE disease activity...
March 26, 2018: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29578532/critical-role-of-ox40-signaling-in-the-tcr-independent-phase-of-human-and-murine-thymic-treg-generation
#13
Prabhakaran Kumar, Alejandra Marinelarena, Divya Raghunathan, Vandhana K Ragothaman, Shikha Saini, Palash Bhattacharya, Jilao Fan, Alan L Epstein, Ajay V Maker, Bellur S Prabhakar
Regulatory T cells (Tregs) play a pivotal role in immune-tolerance, and loss of Treg function can lead to the development of autoimmunity. Natural Tregs generated in the thymus substantially contribute to the Treg pool in the periphery, where they suppress self-reactive effector T cells (Teff) responses. Recently, we showed that OX40L (TNFSF4) is able to drive selective proliferation of peripheral Tregs independent of canonical antigen presentation (CAP-independent) in the presence of low-dose IL-2. Therefore, we hypothesized that OX40 signaling might be integral to the TCR-independent phase of murine and human thymic Treg (tTreg) development...
March 26, 2018: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29560431/cd4-t-cells-react-to-local-increase-of-%C3%AE-synuclein-in-a-pathology-associated-variant-dependent-manner-and-modify-brain-microglia-in-absence-of-brain-pathology
#14
Mads N Olesen, Josefine R Christiansen, Steen Vang Petersen, Poul Henning Jensen, Wojciech Paslawski, Marina Romero-Ramos, Vanesa Sanchez-Guajardo
We have previously shown that immunological processes in the brain during α-synuclein-induced neurodegeneration vary depending on the presence or absence of cell death. This suggests that the immune system is able to react differently to the different stages of α-synuclein pathology. However, it was unclear whether these immune changes were governed by brain processes or by a direct immune response to α-synuclein modifications. We have herein locally increased the peripheral concentration of α-synuclein or its pathology-associated variants, nitrated or fibrillar, to characterize the modulation of the CD4 T cell pool by α-synuclein and brain microglia in the absence of any α-synuclein brain pathology...
January 2018: Heliyon
https://www.readbyqxmd.com/read/29556254/immunological-changes-of-t-helper-cells-in-flow-cytometer-sorted-cd4-t-cells-from-patients-with-hashimoto-s-thyroiditis
#15
Yanying Guo, Jazyra Zynat, Shuqing Xing, Liang Xin, Suli Li, Nurongguli Mammat, Yuan Chen, Limin Zhao, Hongli Zhao, Xinling Wang
Recent incidence rates for Hashimoto's thyroiditis (HT) and hypothyroidism are higher than those of previous studies. Previous studies indicated that T helper cells may have a major role in the pathogenesis and development of HT, but there is no consensus in the literature. The aim of the present study was to explore the peripheral T helper cell response in the different stages of HT. In this cross-sectional study, we performed flow cytometry analysis to determine the various T cell subsets of 389 patients with HT (34 patients with HT who developed overt hypothyroidism, and 148 patients with HT who developed subclinical hypothyroidism), as well as 51 healthy controls...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29549233/comparing-serum-levels-of-th17-and-treg-cytokines-in-women-with-unexplained-recurrent-spontaneous-abortion-and-fertile-women
#16
Narges Roomandeh, AboTaleb Saremi, Javad Arasteh, Fatemeh Pak, Majid Mirmohammadkhani, Parviz Kokhaei, Ahad Zare
BACKGROUND: Increased evidences have shown that unexplained recurrent spontaneous abortion (URSA) is associated with inflammatory responses and breakage of immunological autotolerance. Therefore, the balance between Th17 and Treg cells may elucidate the pathophysiology of URSA. OBJECTIVE: To investigate the serum concentration of regulatory and inflammatory cytokines associated with Treg and Th17 in both normal and URSA females. METHODS: Forty-six women with URSA and 28 non-pregnant control women with at least one successful pregnancy were included...
March 2018: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/29545911/impact-on-early-outcomes-and-immune-reconstitution-of-high-dose-post-transplant-cyclophosphamide-vs-anti-thymocyte-globulin-after-reduced-intensity-conditioning-peripheral-blood-stem-cell-allogeneic-transplantation
#17
Christelle Retière, Catherine Willem, Thierry Guillaume, Henri Vié, Laetitia Gautreau-Rolland, Emmanuel Scotet, Xavier Saulquin, Katia Gagne, Marie C Béné, Berthe-Marie Imbert, Beatrice Clemenceau, Pierre Peterlin, Alice Garnier, Patrice Chevallier
We have compared prospectively the outcome and immune reconstitution of patients receiving either post-transplant cyclophosphamide (PTCY) ( n = 30) or anti-thymocyte globulin ATG ( n = 15) as Graft-versus-host disease (GVHD) prophylaxis after reduced-intensity conditioning (RIC) allogeneic peripheral blood stem cell (PBSC) transplantation (allo-SCT). The outcome and immune reconstitution of patients receiving either of these two regimens were compared prospectively. This study allowed also to investigate the impact of PTCY between haplo-identical vs matched donors and of clofarabine as part of the RIC regimen...
February 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29545795/serial-monitoring-of-immune-markers-being-represented-regulatory-t-cell-t-helper-17-cell-ratio-indicating-tolerance-for-tapering-immunosuppression-after-liver-transplantation
#18
JooYeon Jhun, Seung Hoon Lee, Soon Kyu Lee, Hee Yeon Kim, Eun Sun Jung, Dong Goo Kim, JeongWon Choi, Si Hyun Bae, Seung Kew Yoon, Byung Ha Chung, Chul Woo Yang, Mi-La Cho, Jong Young Choi
Recipients of liver transplantation (LT) require long-term immunosuppressive drug treatment, but lifelong immunosuppressive treatment has severe side effects. It is known that some LT recipients develop immune tolerance, and although the development of such operational tolerance should allow a decrease in the burden of immunosuppressive drug treatment, the factors that indicate operational tolerance are not clear. This study aimed to monitor immunological markers over time in LT recipients to identify those markers indicating the development of operational tolerance...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29541073/the-effect-of-tnf-%C3%AE-on-regulatory-t-cell-function-in-graft-versus-host-disease
#19
REVIEW
Antonella Mancusi, Sara Piccinelli, Andrea Velardi, Antonio Pierini
FoxP3+ regulatory T cells (Tregs) are a subset of CD4+ T cells that can suppress proliferation and effector functions of T cells, B cells, NK cells, and antigen-presenting cells. Treg deficiency causes dramatic immunologic disease in both animal models and humans. As they are capable to suppress the function and the proliferation of conventional CD4+ and CD8+ T cells, Treg-based cell therapies are under evaluation for the treatment of various autoimmune diseases and are currently employed to prevent graft-versus-host disease (GvHD) in clinical trials of hematopoietic stem cell transplantation...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29532571/sirolimus-as-an-alternative-treatment-in-patients-with-granulomatous-lymphocytic-lung-disease-and-humoral-immunodeficiency-with-impaired-regulatory-t-cells
#20
Angela Deyà-Martínez, Ana Esteve-Solé, Natalia Vélez-Tirado, Veronica Celis, Jordi Costa, Maria Cols, Cristina Jou, Alexandru Vlagea, Ana María Plaza-Martin, Manel Juan, Laia Alsina
BACKGROUND: One of the most frequent non-infectious complications of humoral immunodeficiencies with a CVID-like pattern is a particular form of inflammatory lung disease which is called granulomatous-lymphocytic interstitial lung disease (GLILD). Its development worsens patient prognosis, with a significant decrease in survival. Currently there are no unified guidelines regarding its management, and different combinations of immunosuppressants have been used with variable success. METHODS: Clinical and radiological data were collected from patient's medical charts...
March 12, 2018: Pediatric Allergy and Immunology
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