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https://www.readbyqxmd.com/read/28739769/effects-of-different-palliative-jaundice-reducing-methods-on-immunologic-functions-in-patients-with-advanced-malignant-obstructive-jaundice
#1
Kun Tang, Lu-Lu Sui, Gang Xu, Tong Zhang, Qiang Liu, Xiao-Fang Liu
BACKGROUND/AIM: This study aimed to investigate the effects of three treatment methods on the immunological function of patients with advanced malignant obstructive jaundice (MOJ). PATIENTS AND METHODS: Patients with advanced MOJ were randomly divided into three groups according to biliary drainage methods. Detection of levels of multi-indices were investigated in different time periods. RESULTS: After drainage, the levels of complement 3 (C3) and complement 4 (C4) were increased...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739648/hepatocyte-nuclear-factor-1%C3%AE-controls-mitochondrial-respiration-in-renal-tubular-cells
#2
Audrey Casemayou, Audren Fournel, Alessia Bagattin, Joost Schanstra, Julie Belliere, Stéphane Decramer, Dimitri Marsal, Marion Gillet, Nicolas Chassaing, Antoine Huart, Marco Pontoglio, Claude Knauf, Jean-Loup Bascands, Dominique Chauveau, Stanislas Faguer
AKI is a frequent condition that involves renal microcirculation impairment, infiltration of inflammatory cells with local production of proinflammatory cytokines, and subsequent epithelial disorders and mitochondrial dysfunction. Peroxisome proliferator-activated receptor γ coactivator 1-α (PPARGC1A), a coactivator of the transcription factor PPAR-γ that controls mitochondrial biogenesis and function, has a pivotal role in the early dysfunction of the proximal tubule and the subsequent renal repair. Here, we evaluated the potential role of hepatocyte nuclear factor-1β (HNF-1β) in regulating PPARGC1A expression in AKI...
July 24, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28739595/retraction-hypoxia-inducible-factor-hif-1%C3%AE-accumulation-and-hif-target-gene-expression-are-impaired-after-induction-of-endotoxin-tolerance
#3
Stilla Frede, Christian Stockmann, Sandra Winning, Patricia Freitag, Joachim Fandrey
No abstract text is available yet for this article.
August 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28739486/microrna-146a-5p-attenuates-liver-fibrosis-by-suppressing-profibrogenic-effects-of-tgf%C3%AE-1-and-lipopolysaccharide
#4
Yanting Zou, Yu Cai, Di Lu, Yi Zhou, Qunyan Yao, Shuncai Zhang
Liver fibrosis is characterized by proliferation and activation of hepatic stellate cells (HSCs). Transforming growth factor-β1 (TGFβ1) is crucial for liver fibrogenesis, and gut-derived endotoxin (LPS) also plays an important role in liver fibrogenesis. In the present study, we found that microRNA-146a-5p (miR-146a-5p) could regulate TGFβ1/Smad and LPS/NF-κB/Bambi pathways to attenuate liver fibrosis. Downregulated miR-146a-5p and upregulated level of LPS were found in liver of CCl4-treated rats. On cellular level, expression of miR-146a-5p is reduced during primary rat HSCs naturally activation and changed in response to TGFβ1 and/or LPS stimulation in primary rat HSCs and human HSC line LX-2...
July 21, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28738497/oeanolic-acid-protects-against-the-hepatotoxicity-of-d-galactosame-plus-endotoxin-in-mice
#5
Xiao-Li Wan, Yuan-Fu Lu, Shang-Fu Xu, Qin Wu, Jie Liu
Oleanolic acid (OA) is a triterpenoid contained in many herbal medicines. The aim of this study was to investigate the protective effect of OA against D-galactosame plus lipopolysaccharides (D-GalN/LPS)-induced acute liver injury and the underling mechanisms. Mice were randomly divided into normal control with vehicles only (corn oil), D-GalN/LPS only (700mg/10μg/kg, ip), OA-po (200μmol/kg in corn oil, po) plus D-GalN/LPS, and OA-sc (50μmol/kg in 2% tween 80, sc) plus D-GalN/LPS groups. OA pretreatment was conducted twice daily for 4 consecutive days...
July 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28736267/gamma-tocopherol-enriched-supplement-reduces-sputum-eosinophilia-and-endotoxin-induced-sputum-neutrophilia-in-volunteers-with-asthma
#6
Allison J Burbank, Charity G Duran, Yinghao Pan, Patricia Burns, Susan Jones, Qing Jiang, Chao Yang, Sha'Leema Jenkins, Heather Wells, Neil Alexis, Mehmet Kesimer, William D Bennett, Haibo Zhou, David B Peden, Michelle L Hernandez
BACKGROUND: We and others have shown that the gamma tocopherol (γT) isoform of vitamin E has multiple anti-inflammatory and antioxidant actions and that γT supplementation reduces eosinophilic and endotoxin (LPS)-induced neutrophilic airway inflammation in animal models and healthy human volunteers. OBJECTIVE: To determine if γT supplementation reduces eosinophilic airway inflammation and acute neutrophilic response to inhaled LPS challenge in volunteers with asthma...
July 20, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28736259/molecular-epidemiology-and-antimicrobial-susceptibility-of-clostridium-difficile-isolated-from-the-chinese-people-s-liberation-army-general-hospital-in-china
#7
Rui Wang, Hui Xia Chen, Lin Jian Song, Yue Yun Shen, Yan Ping Luo
Clostridium difficile is a gram-positive, spore-forming obligate anaerobe responsible for antibiotic-associated diarrhea. Its virulence is associated with the production of endotoxins A and B and endospores, which can cause symptoms, such as diarrhea, toxic megacolon and pseudomembranous colitis. Given the increasing elderly population and the well-recognized problem of over-prescribing of broad spectrum antibiotics, it is critical to understand molecular epidemiology and antimicrobial susceptibility in China...
July 20, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28736241/oxytocin-release-via-activation-of-trpm2-and-cd38-in-the-hypothalamus-during-hyperthermia-in-mice-implication-for-autism-spectrum-disorder
#8
REVIEW
Haruhiro Higashida, Teruko Yuhi, Shirin Akther, Sarwat Amina, Jing Zhong, Mingkun Liang, Tomoko Nishimura, Hong-Xiang Liu, Olga Lopatina
Oxytocin (OT) is a critical molecule for social recognition that mediates social and emotional behaviors. OT is released during stress and acts as an anxiolytic factor. To know the precise molecular mechanisms underlying OT release into the brain during stress is important. It has been reported that intracellular concentrations of free calcium in the hypothalamic neurons are elevated by simultaneous stimulation of cyclic ADP-ribose (cADPR) and heat. We have reported in vitro and in vivo data that supports the idea that release of OT in the brain of male mice is regulated by cADPR and fever in relation to stress conditions...
July 20, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28733334/application-of-recombinant-factor-c-reagent-for-the-detection-of-bacterial-endotoxins-in-pharmaceutical-products
#9
Jay S Bolden, Kelly R Smith
Recombinant Factor C (rFC) is non-animal derived reagent used to detect bacterial endotoxins in pharmaceutical products. Despite the fact that the reagent was first commercially available nearly 15 years ago, the broad use of rFC in pharmaceutical industry has long been lagging, presumably due to historical single source supplier concerns and the lack of inclusion in worldwide pharmacopeias. Commercial rFC reagents are now available from multiple manufacturers, thus single sourcing is no longer an issue. We report here the successful validation of several pharmaceutical products by an end-point florescence-based endotoxin method using the rFC reagent...
July 20, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28733332/demonstration-of-the-maintaining-the-validated-state-of-a-system-used-to-generate-water-for-injection-by-thermocompression
#10
Alberto Leyva, Iriac Bisquet, Tania de la Cruz, Leoner Del Arco, Joel Ríos, Rodolfo Valdés, Sonny Robles, Fabian Ugarte, Manuel Montané
Water for injection is used in multiple applications in Biopharmaceutical industry. Regarding this, several methods have been use to generate water with this high quality. Within them, the thermos-compression distillation method has been widely employed. Though, reports on the maintenance of the qualification and validation status of thermo-compression systems used for water for injection generation are non-existent in the scientific literature. Therefore, this paper sought to give evidences on continue process verification of a system used for water for injection generation during two years analyzing the level of conductivity, nitrate, total organic carbon (TOC), endotoxins and microbiology in 1284 water samples...
July 20, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28728370/endotoxin-induced-inflammation-disturbs-melatonin-secretion-in-ewe
#11
Andrzej Przemysław Herman, Karolina Wojtulewicz, Joanna Bochenek, Agata Krawczyńska, Hanna Antushevich, Bartosz Pawlina, Marlena Zielińska-Góska, Anna Herman, Katarzyna Romanowicz, Dorota Tomaszewska-Zaremba
The study was designed to determine the effect of intravenous administration of bacterial endotoxin - lipopolysaccharide (LPS) - on the nocturnal secretion of melatonin and on the expression of enzymes of the melatonin biosynthetic pathway in the pineal gland of ewes, taking into account two different photoperiodic conditions: short night (SN; n = 12) and long night (LN; n = 12). Endotoxin administration lowered (P < 0.05) levels of circulating melatonin in animals from LN photoperiod only during the first hour after treatment, while in ewes from SN photoperiod only in the third hour after the injection...
June 27, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28727032/probiotic-supplementation-protects-weaned-pigs-against-enterotoxigenic-escherichia-coli-k88-challenge-and-improves-performance-similar-to-antibiotics
#12
L Pan, P F Zhao, X K Ma, Q H Shang, Y T Xu, S F Long, Y Wu, F M Yuan, X S Piao
These studies evaluated the effects of probiotics (PB) as a potential substitute for antibiotics (AB) on diarrhea in relation to immune responses and intestinal health in weaned pigs challenged with enterotoxigenic (ETEC) K88 (Exp. 1) and the effects of PB on performance and nutrient digestibility in weaned pigs (Exp. 2). In Exp. 1, 24 weaned barrows (4.9 ± 0.4 kg initial BW) were randomly assigned to 1 of 4 treatments. The treatments consisted of pigs fed an unsupplemented corn-soybean meal basal diet and not challenged (NON-C) or challenged with ETEC K88 (CHA-C) on d 9 and pigs fed the same basal diet supplemented with AB (100 mg/kg zinc bacitracin, 50 mg/kg colistin sulfate, and 100 mg/kg olaquindox; CHA-AB) or 500 mg/kg PB ( and ; CHA-PB) and challenged with ETEC K88 on d 9...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28726347/md2-blockage-protects-obesity-induced-vascular-remodeling-via-activating-ampk-nrf2
#13
Lintao Wang, Jibo Han, Peiren Shan, Shengban You, Xuemei Chen, Yiyi Jin, Jingying Wang, Weijian Huang, Yi Wang, Guang Liang
OBJECTIVE: Obesity and increased free fatty acid (FFA) levels are tightly linked with vascular oxidative stress and remodeling. Myeloid differentiation 2 (MD2), an important protein in innate immunity, is requisite for endotoxin lipopolysaccharide responsiveness. This study shows that palmitic acid (PA) also bonds to MD2, initiating cardiac inflammatory injury. However, it is not clear whether MD2 plays a role in noninflammatory systems such as obesity- and FFA-related oxidative stress involved in vascular remodeling and injury...
July 20, 2017: Obesity
https://www.readbyqxmd.com/read/28722325/polymer-mediated-flocculation-of-transient-cho-cultures-as-a-simple-high-throughput-method-to-facilitate-antibody-discovery
#14
Matthew G Schmitt, Yashas Rajendra, Maria D Hougland, Jeffrey S Boyles, Gavin C Barnard
Most biopharmaceutical drugs, especially monoclonal antibodies (mAbs), bispecific antibodies (BsAbs) and Fc-fusion proteins, are expressed using Chinese Hamster Ovary (CHO) cell lines. CHO cells typically yield high product titers and high product quality. Unfortunately, CHO cell lines also generate high molecular weight (HMW) aggregates of the desired product during cell culture along with CHO host cell protein (HCP) and CHO DNA. These immunogenic species, co-purified during Protein A purification, must be removed in a multi-step purification process...
July 19, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28718709/bioaerosols-concentrations-in-working-areas-in-biomethanization-facilities
#15
Marie-Eve Dubuis, Hamza M'Bareche, Marc Veillette, Bouchra Bakhiyi, Joseph Zayed, Jacques Lavoie, Caroline Duchaine
This study sought to fill the gap in information about the type and the concentration of bioaerosols present in the air of biomethanization facilities (BF). Evaluation of bioaerosol composition and concentration was achieved in two biomethanization facilities located in Eastern Canada, during summer and winter. In order to have a thorough understanding of the studied environment, the methodology combined culture of bacteria and molds, qPCR for specific microorganisms, endotoxin quantification and NGS for bacterial diversity...
July 18, 2017: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/28717421/isolation-of-membrane-vesicles-from-prokaryotes-a-technical-and-biological-comparison-reveals-heterogeneity
#16
Priscila Dauros Singorenko, Vanessa Chang, Alana Whitcombe, Denis Simonov, Jiwon Hong, Anthony Phillips, Simon Swift, Cherie Blenkiron
Prokaryotes release membrane vesicles (MVs) with direct roles in disease pathogenesis. MVs are heterogeneous when isolated from bacterial cultures so Density Gradient Centrifugation (DGC) is valuable for separation of MV subgroups from contaminating material. Here we report the technical variability and natural biological heterogeneity seen between DGC preparations of MVs for Mycobacterium smegmatis and Escherichia coli and compare these DGC data with size exclusion chromatography (SEC) columns. Crude preparations of MVs, isolated from cultures by ultrafiltration and ultracentrifugation were separated by DGC with fractions manually collected as guided by visible bands...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28716745/gut-derived-endotoxin-stimulates-factor-viii-secretion-from-endothelial-cells-implications-for-hypercoagulability-in-cirrhosis
#17
Carnevale Roberto, Raparelli Valeria, Nocella Cristina, Bartimoccia Simona, Novo Marta, Severino Anna, De Falco Elena, Cammisotto Vittoria, Pasquale Chiara, Crescioli Clara, Sili Scavalli Antonio, Riggio Oliviero, Basili Stefania, Violi Francesco
BACKGROUND & AIMS: Patients with cirrhosis display enhanced blood levels of factor VIII, which may result in harmful activation of the clotting system; however, the underlying mechanism is unknown. METHODS: We performed a cross-sectional study in cirrhotic patients (n=61) and matched controls (n=61) comparing blood levels of factor VIII, von Willebrand factor (vWf), lipopolysaccharide (LPS) and positivity for Escherichia Coli DNA. Furthermore, we performed an in-vitro study to investigate if LPS, in a range of concentration similar to that found in the peripheral circulation of cirrhotic patients, was able to elicit factor VIII secretion from human umbilical vein endothelial cells (HUVEC)...
July 14, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28715154/the-role-of-oxidative-stress-in-acute-renal-injury-of-newborn-rats-exposed-to-hypoxia-and-endotoxin
#18
Egor Y Plotnikov, Tatiana A Pavlenko, Irina B Pevzner, Ljubava D Zorova, Vasily N Manskikh, Denis N Silachev, Gennady T Sukhikh, Dmitry B Zorov
Neonatal kidney injury is a frequent pathology, especially among premature infants. The search for effective nephroprotection requires the creation of adequate experimental models of nephropathy in newborns. In this study, we explored the development of acute kidney injury (AKI) in neonatal rats during hypoxia or administration of endotoxin. We found that 2-h hypoxia (8% O2 ) and the intraperitoneal injection of 4 mg/kg lipopolysaccharide (LPS) causes the appearance of AKI markers, such as КIM-1 and NGAL in the rat urine after 24 and 72 h of exposure...
July 17, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28714978/%C3%AE-ketoglutarate-orchestrates-macrophage-activation-through-metabolic-and-epigenetic-reprogramming
#19
Pu-Ste Liu, Haiping Wang, Xiaoyun Li, Tung Chao, Tony Teav, Stefan Christen, Giusy Di Conza, Wan-Chen Cheng, Chih-Hung Chou, Magdalena Vavakova, Charlotte Muret, Koen Debackere, Massimiliano Mazzone, Hsien-Da Huang, Sarah-Maria Fendt, Julijana Ivanisevic, Ping-Chih Ho
Glutamine metabolism provides synergistic support for macrophage activation and elicitation of desirable immune responses; however, the underlying mechanisms regulated by glutamine metabolism to orchestrate macrophage activation remain unclear. Here we show that the production of α-ketoglutarate (αKG) via glutaminolysis is important for alternative (M2) activation of macrophages, including engagement of fatty acid oxidation (FAO) and Jmjd3-dependent epigenetic reprogramming of M2 genes. This M2-promoting mechanism is further modulated by a high αKG/succinate ratio, whereas a low ratio strengthens the proinflammatory phenotype in classically activated (M1) macrophages...
July 17, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28714872/immunomodulatory-activity-of-octenyl-succinic-anhydride-modified-porang-amorphophallus-oncophyllus-glucomannan-on-mouse-macrophage-like-j774-1-cells-and-mouse-primary-peritoneal-macrophages
#20
Sellen Gurusmatika, Kosuke Nishi, Eni Harmayani, Yudi Pranoto, Takuya Sugahara
Porang is a local plant of Indonesia, which has a high content of glucomannan. In this study, porang glucomannan (PG) was esterified with octenyl succinic anhydride (OSA) to enhance emulsion properties to be widely used in food industry. OSA-modified PG (OSA-PG) enhanced the phagocytosis activity of macrophage-like J774.1 cells and mouse peritoneal macrophages. In addition, OSA-PG increased the production of IL-6 and TNF-α by enhancing their gene expression. Immunoblot analysis displayed that OSA-PG tended to activate both nuclear factor-κB and mitogen-activated protein kinase cascades...
July 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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