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clathrin endocytosis

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https://www.readbyqxmd.com/read/29686427/a-mobile-endocytic-network-connects-clathrin-independent-receptor-endocytosis-to-recycling-and-promotes-t-cell-activation
#1
Ewoud B Compeer, Felix Kraus, Manuela Ecker, Gregory Redpath, Mayan Amiezer, Nils Rother, Philip R Nicovich, Natasha Kapoor-Kaushik, Qiji Deng, Guerric P B Samson, Zhengmin Yang, Jieqiong Lou, Michael Carnell, Haig Vartoukian, Katharina Gaus, Jérémie Rossy
Endocytosis of surface receptors and their polarized recycling back to the plasma membrane are central to many cellular processes, such as cell migration, cytokinesis, basolateral polarity of epithelial cells and T cell activation. Little is known about the mechanisms that control the organization of recycling endosomes and how they connect to receptor endocytosis. Here, we follow the endocytic journey of the T cell receptor (TCR), from internalization at the plasma membrane to recycling back to the immunological synapse...
April 23, 2018: Nature Communications
https://www.readbyqxmd.com/read/29684527/co-delivery-of-docetaxel-and-gemcitabine-using-pegylated-self-assembled-stealth-nanoparticles-for-improved-breast-cancer-therapy
#2
Varun Kushwah, Sameer S Katiyar, Ashish Kumar Agrawal, Ramesh C Gupta, Sanyog Jain
The present report deals with conjugation of dual drug; docetaxel (DTX) and gemcitabine (GEM) with linker poly-ethylene-glycol (PEG) to develop amphiphilic molecule having self-assembled property. The synthesized conjugate (DTX-PEG-GEM) demonstrated critical micelle concentration (CMC) in the range of 5-10μg/ml which self-assembled to form NPs with size 124.2±5.7. Remarkably higher coumarin-6 (C-6) fluorescence signals observed in case of C-6 loaded NPs, suggested enhanced cellular uptake via clathrin mediated endocytosis...
April 20, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29681477/a-forward-genetic-screen-in-zebrafish-identifies-the-g-protein-coupled-receptor-casr-as-a-modulator-of-sensorimotor-decision-making
#3
Roshan A Jain, Marc A Wolman, Kurt C Marsden, Jessica C Nelson, Hannah Shoenhard, Fabio A Echeverry, Christina Szi, Hannah Bell, Julianne Skinner, Emilia N Cobbs, Keisuke Sawada, Amy D Zamora, Alberto E Pereda, Michael Granato
Animals continuously integrate sensory information and select contextually appropriate responses. Here, we show that zebrafish larvae select a behavioral response to acoustic stimuli from a pre-existing choice repertoire in a context-dependent manner. We demonstrate that this sensorimotor choice is modulated by stimulus quality and history, as well as by neuromodulatory systems-all hallmarks of more complex decision making. Moreover, from a genetic screen coupled with whole-genome sequencing, we identified eight mutants with deficits in this sensorimotor choice, including mutants of the vertebrate-specific G-protein-coupled extracellular calcium-sensing receptor (CaSR), whose function in the nervous system is not well understood...
April 6, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29679667/systemic-delivery-of-bace1-sirna-through-neuron-targeted-nanocomplexes-for-treatment-of-alzheimer-s-disease
#4
Pengzhen Wang, Xiaoyao Zheng, Qian Guo, Peng Yang, Xiaoying Pang, Kang Qian, Wei Lu, Qizhi Zhang, Xinguo Jiang
β-site amyloid precursor protein cleaving enzyme 1 (BACE1) is a key enzyme to cleave the amyloid precursor protein to develop Alzheimer's disease (AD). Reducing BACE1 expression in central neuron through RNA interference technology shows great promise to overcome AD. However, to obtain an efficient and neurons-specific delivery of siRNA against BACE1 through systemic administration remains challenging. Here, we design and prepare siRNA nano-carriers based on PEGylated poly(2-(N,N-dimethylamino) ethyl methacrylate) (PEG-PDMAEMA) modified with both the CGN peptide for blood-brain barrier (BBB) penetration and the Tet1 peptide for neuron-specific binding...
April 18, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29678956/local-actin-polymerization-during-endocytic-carrier-formation
#5
REVIEW
Claudia Hinze, Emmanuel Boucrot
Extracellular macromolecules, pathogens and cell surface proteins rely on endocytosis to enter cells. Key steps of endocytic carrier formation are cargo molecule selection, plasma membrane folding and detachment from the cell surface. While dedicated proteins mediate each step, the actin cytoskeleton contributes to all. However, its role can be indirect to the actual molecular events driving endocytosis. Here, we review our understanding of the molecular steps mediating local actin polymerization during the formation of endocytic carriers...
April 20, 2018: Biochemical Society Transactions
https://www.readbyqxmd.com/read/29668686/a-noncanonical-role-for-dynamin-1-in-regulating-early-stages-of-clathrin-mediated-endocytosis-in-non-neuronal-cells
#6
Saipraveen Srinivasan, Christoph J Burckhardt, Madhura Bhave, Zhiming Chen, Ping-Hung Chen, Xinxin Wang, Gaudenz Danuser, Sandra L Schmid
Dynamin Guanosine Triphosphate hydrolases (GTPases) are best studied for their role in the terminal membrane fission process of clathrin-mediated endocytosis (CME), but they have also been proposed to regulate earlier stages of CME. Although highly enriched in neurons, dynamin-1 (Dyn1) is, in fact, widely expressed along with Dyn2 but inactivated in non-neuronal cells via phosphorylation by glycogen synthase kinase-3 beta (GSK3β) kinase. Here, we study the differential, isoform-specific functions of Dyn1 and Dyn2 as regulators of CME...
April 18, 2018: PLoS Biology
https://www.readbyqxmd.com/read/29661273/irs-1-acts-as-an-endocytic-regulator-of-igf-i-receptor-to-facilitate-sustained-igf-signaling
#7
Yosuke Yoneyama, Peter Lanzerstorfer, Hideaki Niwa, Takashi Umehara, Takashi Shibano, Shigeyuki Yokoyama, Kazuhiro Chida, Julian Weghuber, Fumihiko Hakuno, Shin-Ichiro Takahashi
Insulin-like growth factor-I receptor (IGF-IR) preferentially regulates the long-term IGF activities including growth and metabolism. Kinetics of ligand-dependent IGF-IR endocytosis determines how IGF induces such downstream signaling outputs. Here, we find that the insulin receptor substrate (IRS)-1 modulates how long ligand-activated IGF-IR remains at the cell surface before undergoing endocytosis in mammalian cells. IRS-1 interacts with the clathrin adaptor complex AP2. IRS-1, but not an AP2-binding-deficient mutant, delays AP2-mediated IGF-IR endocytosis after the ligand stimulation...
April 11, 2018: ELife
https://www.readbyqxmd.com/read/29661000/regulation-of-clathrin-mediated-endocytosis
#8
Marcel Mettlen, Ping-Hung Chen, Saipraveen Srinivasan, Gaudenz Danuser, Sandra L Schmid
Clathrin-mediated endocytosis (CME) is the major endocytic pathway in mammalian cells. It is responsible for the uptake of transmembrane receptors and transporters, for remodeling plasma membrane composition in response to environmental changes, and for regulating cell surface signaling. CME occurs via the assembly and maturation of clathrin-coated pits that concentrate cargo as they invaginate and pinch off to form clathrin-coated vesicles. In addition to the major coat proteins, clathrin triskelia and adaptor protein complexes, CME requires a myriad of endocytic accessory proteins and phosphatidylinositol lipids...
April 16, 2018: Annual Review of Biochemistry
https://www.readbyqxmd.com/read/29653223/acid-sensitive-hybrid-polymeric-micelles-containing-a-reversibly-activatable-cell-penetrating-peptide-for-tumor-specific-cytoplasm-targeting
#9
Baoqiang Tang, Jennica L Zaro, Yan Shen, Qian Chen, Yinglan Yu, Pingping Sun, Yanqi Wang, Wei-Chiang Shen, Jiasheng Tu, Chunmeng Sun
Cell-penetrating peptides (CPPs) have become a novel drug delivery system due to their distinct advantages, including high cell transmembrane potency and ability to carry cargo molecules inside cells. However, owing to their cationic charge and non-specificity characteristics, the clinical application of CPPs is limited. In the current study, we engineered a reversibly activatable cell-penetrating peptide (RACPP), containing oligoarginine fused to a pH-sensitive masking sequence via a polyglycine linker ((HE)10 G5 R6 or HE-CPP) with ultra-pH-sensitivity...
April 10, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29652478/isothermal-self-assembly-of-spermidine-dna-nanostructure-complex-as-a-functional-platform-for-cancer-therapy
#10
Dong Wang, Qian Liu, Di Wu, Binfeng He, Jin Li, Chengde Mao, Guansong Wang, Hang Qian
Programmable DNA nanostructure self-assembly offer great potentials in nanomedicine, drug delivery, biosensing and bioimaging. However, the intrinsically negative-charged DNA backbones, the instability of DNA nanostructures in physiological settings pose serious challenges to their practical applications. To overcome this challenge, a strategy that combines the magnesium-free DNA self-assembly and functionalization is proposed in this study. We hypothesize that naturally abundant spermidine may not only mediate the self-assembly of DNA nanostructures, but also shield them from harsh physiological environments...
April 13, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29652249/clathrin-coat-controls-synaptic-vesicle-acidification-by-blocking-vacuolar-atpase-activity
#11
Zohreh Farsi, Sindhuja Gowrisankaran, Matija Krunic, Burkhard Rammner, Andrew Woehler, Eileen M Lafer, Carsten Mim, Reinhard Jahn, Ira Milosevic
Newly-formed synaptic vesicles (SVs) are rapidly acidified by vacuolar adenosine triphosphatases (vATPases), generating a proton electrochemical gradient that drives neurotransmitter loading. Clathrin-mediated endocytosis is needed for the formation of new SVs, yet it is unclear when endocytosed vesicles acidify and refill at the synapse. Here, we isolated clathrin-coated vesicles (CCVs) from mouse brain to measure their acidification directly at the single vesicle level. We observed that the ATP-induced acidification of CCVs was strikingly reduced in comparison to SVs...
April 13, 2018: ELife
https://www.readbyqxmd.com/read/29649664/autophagic-related-cell-death-of-trypanosoma-brucei-induced-by-bacteriocin-as-48
#12
Marta Martínez-García, Jean-Mathieu Bart, Jenny Campos-Salinas, Eva Valdivia, Manuel Martínez-Bueno, Elena González-Rey, Miguel Navarro, Mercedes Maqueda, Rubén Cebrián, José M Pérez-Victoria
The parasitic protozoan Trypanosoma brucei is the causative agent of human African trypanosomiasis (sleeping sickness) and nagana. Current drug therapies have limited efficacy, high toxicity and/or are continually hampered by the appearance of resistance. Antimicrobial peptides have recently attracted attention as potential parasiticidal compounds. Here, we explore circular bacteriocin AS-48's ability to kill clinically relevant bloodstream forms of T. brucei gambiense, T. brucei rhodesiense and T. brucei brucei...
March 12, 2018: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/29649590/effective-intracellular-delivery-and-th1-immune-response-induced-by-ovalbumin-loaded-in-ph-responsive-polyphosphazene-polymersomes
#13
Menghua Gao, Yan Peng, Liming Jiang, Liyan Qiu
A polymersome system for delivering protein antigen ovalbumin (OVA) based on amphiphilic polyphosphazene grafting with N,N-diisopropylethylenediamine (DPA) and poly(ethylene glycol) (PEG) groups (poly[(DPA)m (PEG)n phosphazene], PEDP) was designed and constructed. The 200~240nm-size OVA-loaded polymersomes displayed high stability at physiological pH, slow internalization through clathrin-mediated endocytosis pathway, and then a pH-triggered sustained OVA release in acidic environment, leading to extensive antigen access to cytosol...
April 9, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29648779/the-role-of-membrane-curvature-in-nanoscale-topography-induced-intracellular-signaling
#14
Hsin-Ya Lou, Wenting Zhao, Yongpeng Zeng, Bianxiao Cui
Over the past decade, there has been growing interest in developing biosensors and devices with nanoscale and vertical topography. Vertical nanostructures induce spontaneous cell engulfment, which enhances the cell-probe coupling efficiency and the sensitivity of biosensors. Although local membranes in contact with the nanostructures are found to be fully fluidic for lipid and membrane protein diffusions, cells appear to actively sense and respond to the surface topography presented by vertical nanostructures...
April 12, 2018: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29643986/ajuba-receptor-mediates-the-internalization-of-tumor-secreted-grp78-into-macrophages-through-different-endocytosis-pathways
#15
Xiaoqin La, Lichao Zhang, Hanqing Li, Zhuoyu Li, Guisheng Song, Peng Yang, Yufei Yang
Glucose-regulated protein 78 (GRP78), an ER chaperone, is overexpressed in cancer cells. Solid tumor cells can secrete GRP78 that can promote tumor angiogenesis, differentiation of bone marrow-derived mesenchymal stem cells, tumor cell proliferation and polarization of tumor-associated macrophages. However, the mechanism by which GRP78 functions as a tumor promoter either by staying on the membrane to stimulate intracellular signals or directly entering into cytosolic remains unknown. Here, we reported that an endotoxin-free His-GRP78 protein was purified in vitro that simulates original secreted GRP78...
March 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29643075/widespread-changes-in-transcriptome-profile-of-human-mesenchymal-stem-cells-induced-by-two-dimensional-nanosilicates
#16
James K Carrow, Lauren M Cross, Robert W Reese, Manish K Jaiswal, Carl A Gregory, Roland Kaunas, Irtisha Singh, Akhilesh K Gaharwar
Two-dimensional nanomaterials, an ultrathin class of materials such as graphene, nanoclays, transition metal dichalcogenides (TMDs), and transition metal oxides (TMOs), have emerged as a new generation of materials due to their unique properties relative to macroscale counterparts. However, little is known about the transcriptome dynamics following exposure to these nanomaterials. Here, we investigate the interactions of 2D nanosilicates, a layered clay, with human mesenchymal stem cells (hMSCs) at the whole-transcriptome level by high-throughput sequencing (RNA-seq)...
April 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29623549/defect-related-luminescent-bur-like-hydroxyapatite-microspheres-induced-apoptosis-of-mc3t3-e1-cells-by-lysosomal-and-mitochondrial-pathways
#17
REVIEW
Yi Jin, Shizhu Chen, Nan Li, Yajing Liu, Gong Cheng, Cuimiao Zhang, Shuxiang Wang, Jinchao Zhang
When orthopedic joints coated by hydroxyapatite (HA) were implanted in the human body, they release wear debris into the surrounding tissues. The generation and accumulation of wear particles will induce aseptic loosening. However, the potential bioeffect and mechanism of HA-coated orthopedic implants on bone cells are poorly understood. In this study, defect-related luminescent bur-like hydroxyapatite (BHA) microspheres with the average diameter of 7-9 μm which are comparable to that of the wear-debris particles from aseptically loosened HA implants or HA debris have been synthesized by hydrothermal synthesis and the MC3T3-E1 cells were set as a cells model to study the potential bioeffect and mechanism of BHA microspheres...
March 30, 2018: Science China. Life Sciences
https://www.readbyqxmd.com/read/29618727/rule-based-modelling-provides-an-extendable-framework-for-comparing-candidate-mechanisms-underpinning-clathrin-polymerisation
#18
Anatoly Sorokin, Katharina F Heil, J Douglas Armstrong, Oksana Sorokina
Polymerisation of clathrin is a key process that underlies clathrin-mediated endocytosis. Clathrin-coated vesicles are responsible for cell internalization of external substances required for normal homeostasis and life -sustaining activity. There are several hypotheses describing formation of closed clathrin structures. According to one of the proposed mechanisms cage formation may start from a flat lattice buildup on the cellular membrane, which is later transformed into a curved structure. Creation of the curved surface requires rearrangement of the lattice, induced by additional molecular mechanisms...
April 4, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29615680/water-soluble-metalated-covalent-organic-nanobelts-with-improved-bioavailability-for-protein-transportation
#19
Weifu Kong, Jiaxun Wan, Supawadee Namuangruk, Jia Guo, Changchun Wang
An available pathway to prepare the ionized covalent organic nanosheets (iCONs) has been proposed by a metal-assisted aqueous-phase exfoliation route from covalent organic frameworks. The soluble and belt-shaped iCONs could immobilize a large quantity of proteins (2.73 mg/mg, BSA/iCONs) and hence serve as transporters to enhance the protein uptake by cancer cells. Meanwhile, their energy-dependent endocytosis pathway via clathrin-coated pits has been proved as well.
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29610346/flavivirus-internalization-is-regulated-by-a-size-dependent-endocytic-pathway
#20
Brent A Hackett, Sara Cherry
Flaviviruses enter host cells through the process of clathrin-mediated endocytosis, and the spectrum of host factors required for this process are incompletely understood. Here we found that lymphocyte antigen 6 locus E (LY6E) promotes the internalization of multiple flaviviruses, including West Nile virus, Zika virus, and dengue virus. Perhaps surprisingly, LY6E is dispensable for the internalization of the endogenous cargo transferrin, which is also dependent on clathrin-mediated endocytosis for uptake. Since viruses are substantially larger than transferrin, we reasoned that LY6E may be required for uptake of larger cargoes and tested this using transferrin-coated beads of similar size as flaviviruses...
April 2, 2018: Proceedings of the National Academy of Sciences of the United States of America
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