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clathrin endocytosis

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https://www.readbyqxmd.com/read/29028548/enhanced-antitumor-efficacy-of-arginine-modified-amphiphilic-nanoparticles-co-delivering-doxorubicin-and-isur-pdna-via-the-multiple-synergistic-effect
#1
Yudong Song, Cui Tang, Chunhua Yin
Arginine and α-tocopherol succinate (α-TOS) double grafted N-trimethyl chitosan chloride (TMC) nanoparticles (TAS NPs) were designed and developed for effective co-delivery of doxorubicin (DOX) and Survivin shRNA-expressing pDNA (iSur-pDNA). With DOX loading into the hydrophobic core and iSur-pDNA combining to the hydrophilic shell, TAS/DOX/pDNA NPs demonstrated favorable structural stability and sustained release properties in vitro. With the special non-clathrin-dependent endocytosis, TAS/DOX/pDNA NPs presented higher cellular uptake and mainly distributed in ER and Golgi rather than lysosomes following internalization...
October 4, 2017: Biomaterials
https://www.readbyqxmd.com/read/29026088/characteristic-rotational-behaviors-of-rod-shaped-cargo-revealed-by-automated-five-dimensional-single-particle-tracking
#2
Kuangcai Chen, Yan Gu, Wei Sun, Bin Dong, Gufeng Wang, Xinxin Fan, Tian Xia, Ning Fang
We report an automated single particle tracking technique for tracking the x, y, z coordinates, azimuthal and elevation angles of anisotropic plasmonic gold nanorod probes in live cells. These five spatial coordinates are collectively referred to as 5D. This method overcomes a long-standing challenge in distinguishing rotational motions from translational motions in the z-axis in differential interference contrast microscopy to result in full disclosure of nanoscale motions with high accuracy. Transferrin-coated endocytic gold nanorod cargoes initially undergo active rotational diffusion and display characteristic rotational motions on the membrane...
October 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/29023475/de-novo-transcriptome-sequencing-of-isaria-cateniannulata-and-comparative-analysis-of-gene-expression-in-response-to-heat-and-cold-stresses
#3
Dingfeng Wang, Liangde Li, Guangyuan Wu, Liette Vasseur, Guang Yang, Pengrong Huang
Isaria cateniannulata is a very important and virulent entomopathogenic fungus that infects many insect pest species. Although I. cateniannulata is commonly exposed to extreme environmental temperature conditions, little is known about its molecular response mechanism to temperature stress. Here, we sequenced and de novo assembled the transcriptome of I. cateniannulata in response to high and low temperature stresses using Illumina RNA-Seq technology. Our assembly encompassed 17,514 unigenes (mean length = 1,197 bp), in which 11,445 unigenes (65...
2017: PloS One
https://www.readbyqxmd.com/read/29021582/novel-mechanism-of-regulation-of-the-5-lipoxygenase-leukotriene-b4-pathway-by-high-density-lipoprotein-in-macrophages
#4
Shigeyasu Tsuda, Masakazu Shinohara, Toshihiko Oshita, Manabu Nagao, Nobuaki Tanaka, Takeshige Mori, Tetsuya Hara, Yasuhiro Irino, Ryuji Toh, Tatsuro Ishida, Ken-Ichi Hirata
High-density lipoprotein (HDL) interacts with various cells, particularly macrophages, in functional cell-HDL interactions. Here, we found that HDL protein quality and lipid quality play critical roles in HDL functions. HDL fractions from healthy volunteers (HDLHealthy) and patients with recurrent coronary atherosclerotic disease (HDLCAD) were prepared. To analyse functional HDL-macrophage interactions, macrophages were co-incubated with each HDL, and lipid mediator production was assessed by liquid chromatography/mass spectrometry-based metabololipidomics...
October 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29021339/phosphorylation-regulates-connexin43-zo-1-binding-and-release-an-important-step-in-gap-junction-turnover
#5
Anastasia F Thévenin, Rachel A Margraf, Charles G Fisher, Rachael M Kells-Andrews, Matthias M Falk
To investigate whether connexin phosphorylation regulates ZO-1's known role in gap junction (GJ) function, we generated and analyzed a series of phosphomimetic and phosphorylation-dead mutants by exchanging known conserved regulatory serine (S) residues 255, 279/282, 365, 368, and 373 located in the C-terminal domain of connexin43 (Cx43) into glutamic acid (E) or alanine (A) residues. All connexin mutants were translated into stable, full-length proteins and assembled into GJs when expressed in HeLa or MDCK cells...
October 11, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29017779/targeted-inhibition-of-hantavirus-replication-and-intracranial-pathogenesis-by-a-chimeric-protein-delivered-sirna
#6
Jie Yang, Ji-Feng Sun, Ting-Ting Wang, Xiao-Hong Guo, Jun-Xia Wei, Lin-Tao Jia, An-Gang Yang
Hantavirus (HV) infection, which underlies hantavirus hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome, remains to be a severe clinical challenge. Here, we synthesized small interfering RNAs (siRNAs) that target the encoding sequences of HV strain 76-118, and validated their inhibitory role in virus replication in HV-infected monkey kidney Vero E6 cells. A chimeric protein, 3G1-Cκ-tP, consisting of a single-chain antibody fragment (3G1) against the HV surface envelop glycoprotein, the constant region of human immunoglobulin κ chain (Cκ), and truncated protamine (amino acids 8-29, tP), was further generated...
October 7, 2017: Antiviral Research
https://www.readbyqxmd.com/read/29017031/angiopoietin-like-4-is-a-wnt-signaling-antagonist-that-promotes-lrp6-turnover
#7
Nadine Kirsch, Ling-Shih Chang, Stefan Koch, Andrey Glinka, Christine Dolde, Gabriele Colozza, Maria D J Benitez, Edward M De Robertis, Christof Niehrs
Angiopoietin-like 4 (ANGPTL4) is a secreted signaling protein that is implicated in cardiovascular disease, metabolic disorder, and cancer. Outside of its role in lipid metabolism, ANGPTL4 signaling remains poorly understood. Here, we identify ANGPTL4 as a Wnt signaling antagonist that binds to syndecans and forms a ternary complex with the Wnt co-receptor Lipoprotein receptor-related protein 6 (LRP6). This protein complex is internalized via clathrin-mediated endocytosis and degraded in lysosomes, leading to attenuation of Wnt/β-catenin signaling...
October 9, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28993197/increased-cellular-uptake-of-peptide-modified-pegylated-gold-nanoparticles
#8
Bo He, Yuan Zhang, Qiang Zhang, Bing He, Wenbing Dai, Xueqing Wang, Dan Yang, Mengmeng Qin, Hua Zhang, Changcheng Yin
Gold nanoparticles are promising drug delivery vehicles for nucleic acids, small molecules, and proteins, allowing various modifications on the particle surface. However, the instability and low bioavailability of gold nanoparticles compromise their clinical application. Here, we functionalized gold nanoparticles with CPP fragments (CALNNPFVYLI, CALRRRRRRRR) through sulfhydryl PEG to increase their stability and bioavailability. The resulting gold nanoparticles were characterized with transmission electron microscopy (TEM), dynamic light scattering (DLS), UV-visible spectrometry and X-ray photoelectron spectroscopy (XPS), and the stability in biological solutions was evaluated...
October 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28985001/gbf1-and-arf1-function-in-vesicular-trafficking-lipid-homeostasis-and-organelle-dynamics
#9
REVIEW
Beata Kaczmarek, Jean-Marc Verbavatz, Catherine L Jackson
The Arf small G proteins act as molecular switches to coordinate multiple downstream pathways that regulate membrane dynamics. Their activation is spatially and temporally controlled by the guanine nucleotide exchange factors (GEFs). Members of the evolutionarily conserved GBF/Gea family of Arf GEFs are well known for their roles in formation of coat protein complex I (COPI) vesicles, essential for maintaining the structure and function of the Golgi apparatus. However, studies over the past ten years have found new functions for these GEFs, along with their substrate Arf1, in lipid droplet metabolism, clathrin-independent endocytosis, signaling at the plasma membrane, mitochondrial dynamics and transport along microtubules...
October 6, 2017: Biology of the Cell
https://www.readbyqxmd.com/read/28982974/protein-kinase-a-induced-internalization-of-slack-channels-from-the-neuronal-membrane-occurs-by-adaptor-protein-2-clathrin-mediated-endocytosis
#10
Sushmitha Gururaj, Katherine M Evely, Kerri D Pryce, Jun Li, Jun Qu, Arin Bhattacharjee
The sodium-activated potassium (KNa) channel Kcnt1 (Slack) is abundantly expressed in nociceptor (pain sending) neurons of the dorsal root ganglion (DRG), where they transmit the large outward conductance IKNa and arbitrate membrane excitability. Slack channel expression at the DRG membrane is necessary for their characteristic firing accommodation during maintained stimulation, and reduced membrane channel density causes hyperexcitability. We have previously shown that in a pro-inflammatory state, a decrease in membrane channel expression leading to reduced Slack-mediated IKNa expression underlies DRG sensitization...
October 5, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28982131/mex3c-interacts-with-adaptor-related-protein-complex-2-and-involves-in-mir-451a-exosomal-sorting
#11
Pin Lu, Huanhuan Li, Ning Li, Ravi N Singh, Colin E Bishop, Xiangxian Chen, Baisong Lu
Some RNA species, especially microRNAs, are non-randomly sorted into exosomes, but how selectivity of RNA exosomal sorting is achieved is unknown. We found that all three variants of RNA-binding ubiquitin E3 ligase (MEX3C)-MEX3C-1, MEX3C-2, and MEX3C-3 -interact with adaptor-related protein complex 2 (AP-2), a cargo adaptor in clathrin-mediated endocytosis. MEX3C's C-terminal RING finger domain and the hnRNP K homology (KH) domain shared by the three MEX3C variants are both necessary for MEX3C/AP-2 interaction...
2017: PloS One
https://www.readbyqxmd.com/read/28979185/the-membrane-glycoprotein-m6a-endocytic-recycling-pathway-involves-clathrin-mediated-endocytosis-and-affects-neuronal-synapses
#12
Micaela D Garcia, Karina Formoso, Gabriela I Aparicio, Alberto C C Frasch, Camila Scorticati
Single point mutations or variations in the expression of the gene encoding the neuronal glycoprotein M6a have been associated with psychiatric disorders such as Alzheimer's disease, depression and schizophrenia. In cultured neurons, M6a positively contributes to neurite extension, axon guidance, filopodia/spine outgrowth, and synapse formation. The endocytic processes of neuronal membrane proteins are linked to the differentiation, growth, signaling and plasticity of neurons. However, the roles of M6a and the precise mechanisms through which M6a internalizes and recycles back to the neuronal membrane are unknown...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28974697/curcumin-encapsulated-zeolitic-imidazolate-frameworks-as-stimuli-responsive-drug-delivery-system-and-their-interaction-with-biomimetic-environment
#13
Ashish Tiwari, Ashutosh Singh, Neha Garg, Jaspreet K Randhawa
Metal organic frameworks (MOFs) exhibit unique features of finely tunable pore structures, excellent chemical stability and flexible surface structural functionality, making them advantageous for a wide range of applications including energy storage, compound separation, catalysis, and drug delivery. The present work enlightens a novel approach of single step fabrication of CCM-ZIF-8 as a drug carrier and its application as stimuli responsive drug delivery systems via external stimuli involving change in pH and in presence of biomimetic cell membrane like environment using liposomes and SDS micelles...
October 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28972287/eps15r-and-clathrin-regulate-ephb2-mediated-cell-repulsion
#14
Emma Evergren, Neville Cobbe, Harvey T McMahon
Expression of Eph receptors and their ligands, the ephrins, have important functions in boundary formation and morphogenesis in both adult and embryonic tissue. The EphB receptors and ephrinB ligands are transmembrane proteins that are expressed in different cells and their interaction drives cell repulsion. For cell repulsion to occur, trans-endocytosis of the inter-cellular receptor-ligand EphB-ephrinB complex is required. The molecular mechanism underlying trans-endocytosis is poorly defined. Here we show that the process is clathrin- and Eps15R-mediated using Co115 colorectal cell lines stably expressing EphB2 and ephrinB1...
October 3, 2017: Traffic
https://www.readbyqxmd.com/read/28967893/a-synthetic-intrabody-based-selective-and-generic-inhibitor-of-gpcr-endocytosis
#15
Eshan Ghosh, Ashish Srivastava, Mithu Baidya, Punita Kumari, Hemlata Dwivedi, Kumari Nidhi, Ravi Ranjan, Shalini Dogra, Akiko Koide, Prem N Yadav, Sachdev S Sidhu, Shohei Koide, Arun K Shukla
Beta-arrestins (βarrs) critically mediate desensitization, endocytosis and signalling of G protein-coupled receptors (GPCRs), and they scaffold a large number of interaction partners. However, allosteric modulation of their scaffolding abilities and direct targeting of their interaction interfaces to modulate GPCR functions selectively have not been fully explored yet. Here we identified a series of synthetic antibody fragments (Fabs) against different conformations of βarrs from phage display libraries. Several of these Fabs allosterically and selectively modulated the interaction of βarrs with clathrin and ERK MAP kinase...
October 2, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28961039/clathrin-mediated-integrin-%C3%AE-iib%C3%AE-3-trafficking-controls-platelet-spreading
#16
Wen Gao, Panlai Shi, Xue Chen, Lin Zhang, Junling Liu, Xuemei Fan, Xinping Luo
Dynamic endocytic and exocytic trafficking of integrins is an important mechanism for cell migration, invasion, and cytokinesis. Endocytosis of integrin can be classified as clathrin dependent and clathrin independent manners. And rapid delivery of endocytic integrins back to the plasma membrane is key intracellular signals and is indispensable for cell movement. Integrin αIIbβ3 plays a critical role in thrombosis and hemostasis. Although previous studies have demonstrated that internalization of fibrinogen-bound αIIbβ3 may regulate platelet activation, the roles of endocytic and exocytic trafficking of integrin αIIbβ3 in platelet activation are unclear...
September 29, 2017: Platelets
https://www.readbyqxmd.com/read/28960358/lipid-remodeling-in-neuroendocrine-secretion
#17
REVIEW
Stéphane Gasman, Nicolas Vitale
Neuroendocrine cells secrete hormones and polypeptides through a complex membrane trafficking process that involves the transport of specific organelles, called large dense core secretory granules, from the Golgi apparatus to specialized sites at the plasma membrane where these vesicles are successively exocytosed and recaptured by endocytosis through tightly coupled reactions. The minimal machinery required for exocytosis has been defined as SNARE proteins associated with few accessory proteins. On the other side, clathrin and dynamin constitute major components of some of the most important endocytotic pathways...
September 27, 2017: Biology of the Cell
https://www.readbyqxmd.com/read/28957392/a-highly-sensitive-high-throughput-assay-for-dynamin-s-basal-gtpase-activity
#18
Aparna Mohanakrishnan, Triet Vincent M Tran, Meera Kumar, Hong Chen, Bruce A Posner, Sandra L Schmid
Clathrin-mediated endocytosis is the major pathway by which cells internalize materials from the external environment. Dynamin, a large multidomain GTPase, is a key regulator of clathrin-mediated endocytosis. It assembles at the necks of invaginated clathrin-coated pits and, through GTP hydrolysis, catalyzes scission and release of clathrin-coated vesicles from the plasma membrane. Several small molecule inhibitors of dynamin's GTPase activity, such as Dynasore and Dyngo-4a, are currently available, although their specificity has been brought into question...
2017: PloS One
https://www.readbyqxmd.com/read/28956766/porcine-hemagglutinating-encephalomyelitis-virus-enters-neuro-2a-cells-via-clathrin-mediated-endocytosis-in-a-rab5-cholesterol-and-ph-dependent-manner
#19
Zi Li, Kui Zhao, Yungang Lan, Xiaoling Lv, Shiyu Hu, Jiyu Guan, Huijun Lu, Jing Zhang, Junchao Shi, Yawen Yang, Deguang Song, Feng Gao, Wenqi He
Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that invades the central nervous system (CNS) in piglets. Although important progress has been made towards understanding the biology of PHEV, many aspects of its life cycle remain obscure. Here, we dissected the molecular mechanism underlying cellular entry and intracellular trafficking of PHEV in mouse neuroblastoma (Neuro-2a) cells. We first performed a thin-section transmission electron microscopy (TEM) assay to characterize the kinetics of PHEV, and found that viral entry and transfer occur via membranous-coating-mediated endo-/exocytosis...
September 27, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28954824/new-tools-for-hot-wiring-clathrin-mediated-endocytosis-with-temporal-and-spatial-precision
#20
Laura A Wood, Gabrielle Larocque, Nicholas I Clarke, Sourav Sarkar, Stephen J Royle
Clathrin-mediated endocytosis (CME) is the major route of receptor internalization at the plasma membrane. Analysis of constitutive CME is difficult because the initiation of endocytic events is unpredictable. When and where a clathrin-coated pit will form and what cargo it will contain are difficult to foresee. Here we describe a series of genetically encoded reporters that allow the initiation of CME on demand. A clathrin-binding protein fragment ("hook") is inducibly attached to an "anchor" protein at the plasma membrane, which triggers the formation of new clathrin-coated vesicles...
September 27, 2017: Journal of Cell Biology
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