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clathrin endocytosis

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https://www.readbyqxmd.com/read/28535473/length-effect-of-methoxy-poly-ethylene-oxide-b-poly-%C3%AE%C2%B5-caprolactone-g-poly-methacrylic-acid-copolymers-on-cisplatin-delivery
#1
Hsuan-Ying Chen, Yu-Lun Lo, Pei-Ling Wu, Pei-Chi Lo, Li-Fang Wang
Novel comb-shaped amphiphilic copolymers based on methoxy poly(ethylene glycol)-b-[poly(ε-caprolactone)-g-poly(methacrylic acid)] (MPCL-g-pMAA), were synthesized via ring opening polymerization (ROP) and atom transfer radical polymerization (ATRP) for drug delivery systems. MPCL-g-pMAAs with various MAA repeating units self-assemble into a core-shell structure in an aqueous solution. Critical aggregation concentrations range within 5.6×10(-3)-7.0×10(-2)mg/mL in double deionized water and 8.9×10(-3)-7.0×10(-2)mg/mL in phosphate buffered saline of pH 7...
May 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28527895/suppression-of-pmrab11-inhibits-yhv-infection-in-penaeus-monodon
#2
Akechai Kongprajug, Sakol Panyim, Chalermporn Ongvarrasopone
Yellow head virus (YHV) is one of the most serious pathogens that causes worldwide shrimp production loss. It enters the cells via clathrin-mediated endocytosis and utilizes small GTPase Rab proteins such as PmRab5 and PmRab7 for intracellular trafficking. In this study, molecular cloning and functional analysis of Rab11 during YHV infection were investigated. PmRab11 cDNA was cloned by Rapid amplification of cDNA ends (RACEs). It contained two forms of sizes 1200 and 1050 bp distinct at the 5' UTR. The coding region of PmRab11 was 645 bp, encoding 214 amino acids...
May 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28526602/palmitoyl-ascorbate-and-doxorubicin-co-encapsulated-liposome-for-synergistic-anticancer-therapy
#3
Yue Yang, Xiaoyu Lu, Qi Liu, Yu Dai, Xiaojie Zhu, Yanli Wen, Jiaqiu Xu, Yang Lu, Di Zhao, Xijing Chen, Ning Li
Combination therapy with two drugs and nanoparticle-based drug delivery systems are widely applied to reduce the adverse effects of traditional treatment by chemotherapeutic drugs. Palmitoyl ascorbate (PA) as a lipophilic derivative of ascorbic acid shows the advantages in cancer treatment. The aim of the study was to prepare a doxorubicin (DOX) and PA co-loaded liposome to synergistically treat tumor and effectively alleviate the toxicity caused by DOX. The effects were evaluated by in vitro and in vivo studies...
May 16, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28520088/clathrin-mediated-endocytosis-is-required-for-ane-30-100k-induced-autophagy
#4
Mei-Huei Lin, Young-Chau Liu, Shyun-Yeu Liu, Fang-Chi Chen, Pei-Jung Yang, Guan-Hua Li, Pang-Yen Liu, Ching-Yu Yen
BACKGROUND: We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. METHODS: We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells...
May 18, 2017: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/28515429/endocytic-uptake-of-monomeric-amyloid-%C3%AE-peptides-is-clathrin-and-dynamin-independent-and-results-in-selective-accumulation-of-a%C3%AE-1-42-compared-to-a%C3%AE-1-40
#5
Emelie Wesén, Gavin D M Jeffries, Maria Matson Dzebo, Elin K Esbjörner
Intraneuronal accumulation of amyloid-β (Aβ) peptides represent an early pathological feature in Alzheimer's disease. We have therefore utilized flow cytometry and confocal microscopy in combination with endocytosis inhibition to explore the internalisation efficiency and uptake mechanisms of Aβ(1-40) and Aβ(1-42) monomers in cultured SH-SY5Y cells. We find that both variants are constitutively internalised via endocytosis and that their uptake is proportional to cellular endocytic rate. Moreover, SH-SY5Y cells internalise consistently twice the amount of Aβ(1-42) compared to Aβ(1-40); an imaging-based quantification showed that cells treated with 1 µM peptide for 8 h contained 800,000 peptides of Aβ(1-42) and 400,000 of Aβ(1-40)...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28507272/phosphatidylinositol-5-phosphate-4-kinase-regulates-early-endosomal-dynamics-during-clathrin-mediated-endocytosis
#6
Kumari Kamalesh, Deepti Trivedi, Sarah Toscano, Sanjeev Sharma, Sourav Kolay, Padinjat Raghu
Endocytic turnover is essential to regulate the protein composition and function of the plasma membrane thus regulating the plasma membrane levels of many receptors. In Drosophila photoreceptors, photon absorption by the GPCR rhodopsin 1 (Rh1) triggers its endocytosis by clathrin-mediated endocytosis (CME). We find that CME of Rh1 is regulated by phosphatidylinositol 5 phosphate 4-kinase (PIP4K). Flies lacking PIP4K show mislocalization of Rh1 on expanded endomembranes within the cell body. This mislocalization of Rh1 was dependent on the formation of an expanded Rab5 compartment...
May 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28497682/size-dependent-regulation-of-intracellular-trafficking-of-polystyrene-nanoparticle-based-drug-delivery-carriers
#7
Ting Wang, Lu Wang, Xiaoming Li, Xingjie Hu, Yuping Han, Yao Luo, Zejun Wang, Qian Li, Ali Aldalbahi, Lihua Wang, Shiping Song, Chunhai Fan, Yun Zhao, Maolin Wang, Nan Chen
Nanoparticles (NPs) have shown great promise as intracellular imaging probes or nanocarriers and play increasing role in biomedical applications. A detailed understanding of how NPs get "in and out" of cells is important for developing new nanomaterials with improved selectivity and less cytotoxicity. Both physical and chemical characteristics have proven to regulate the cellular uptake of nanoparticles. However, the exocytosis process and its regulation are less explored. Herein, we investigated the size-regulated endocytosis and exocytosis of carboxylated polystyrene (PS) nanoparticles...
May 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28495363/app-aplp2-and-lrp1-interact-with-pcsk9-but-are-not-required-for-pcsk9-mediated-degradation-of-the-ldlr-in-vivo
#8
Ting Fu, YangYang Guan, Junjie Xu, Yan Wang
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a secreted protein that post-transcriptionally regulates the levels of hepatic low-density lipoprotein receptors (LDLRs). PCSK9 binds to the extracellular domain of the LDLR, and the PCSK9-LDLR complex is internalized through canonical clathrin-dependent endocytosis and then delivered to lysosomes for degradation. The mechanism by which PCSK9 blocks recycling of the LDLR has not been fully defined. Previous reports showed that amyloid precursor-like protein 2 (APLP2) interacts with PCSK9, but its role in PCSK9-mediated LDLR degradation remains controversial...
May 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28491823/comparative-proteomics-reveals-differences-in-host-pathogen-interaction-between-infectious-and-commensal-relationship-with-campylobacter-jejuni
#9
Nieves Ayllón, Ángeles Jiménez-Marín, Héctor Argüello, Sara Zaldívar-López, Margarita Villar, Carmen Aguilar, Angela Moreno, José De La Fuente, Juan J Garrido
Campylobacter jejuni is the leading food-borne poisoning in industrialized countries. While the bacteria causes disease in humans, it merely colonizes the gut in poultry or pigs, where seems to establish a commensal relationship. Until now, few studies have been conducted to elucidate the relationship between C. jejuni and its different hosts. In this work, a comparative proteomics approach was used to identify the underlying mechanisms involved in the divergent outcome following C. jejuni infection in human and porcine host...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28490792/length-effects-on-the-dynamic-process-of-cellular-uptake-and-exocytosis-of-single-walled-carbon-nanotubes-in-murine-macrophage-cells
#10
Xuejing Cui, Bin Wan, Yu Yang, Xiaomin Ren, Liang-Hong Guo
Cellular uptake and exocytosis of SWCNTs are fundamental processes determining their intracellular concentration and effects. Despite the great potential of acid-oxidized SWCNTs in biomedical field, understanding of the influencing factors on these processes needs to be deepened. Here, we quantitatively investigated uptake and exocytosis of SWCNTs in three lengths-630 (±171) nm (L-SWCNTs), 390 (±50) nm (M-SWCNTs), and 195 (±63) nm (S-MWCNTs) in macrophages. The results showed that the cellular accumulation of SWCNTs was a length-independent process and non-monotonic in time, with the most SWCNTs (3950 fg/cell) accumulated at 8 h and then intracellular SWCNTs dropped obviously with time...
May 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28484480/single-event-resolution-of-plant-plasma-membrane-protein-endocytosis-by-tirf-microscopy
#11
Alexander Johnson, Grégory Vert
Endocytosis is a key process in the internalization of extracellular materials and plasma membrane proteins, such as receptors and transporters, thereby controlling many aspects of cell signaling and cellular homeostasis. Endocytosis in plants has an essential role not only for basic cellular functions but also for growth and development, nutrient delivery, toxin avoidance, and pathogen defense. The precise mechanisms of endocytosis in plants remain quite elusive. The lack of direct visualization and examination of single events of endocytosis has greatly hampered our ability to precisely monitor the cell surface lifetime and the recruitment profile of proteins driving endocytosis or endocytosed cargos in plants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28474392/detection-and-characterization-of-individual-endocytosis-of-ampa-type-glutamate-receptor-around-postsynaptic-membrane
#12
Shumpei Fujii, Hiromitsu Tanaka, Tomoo Hirano
Synaptic plasticity such as long-term depression (LTD) has been regarded as a cellular mechanism of learning and memory. LTD is expressed by the decrease in number of postsynaptic AMPA-type receptor (AMPAR) at glutamatergic synapses. Although endocytosis is known to play an essential role in the decrease in AMPAR on postsynaptic membrane, the difficulty to detect individual endocytic events hampered clarification of AMPAR dynamics around synapses. Previously, we developed a method to induce formation of postsynaptic-like membrane (PSLM) on the glass surface and observed pHluorin-tagged AMPAR around PSLM with total internal reflection fluorescence microscopy...
May 5, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28473656/efficient-intracellular-delivery-makes-cancer-cells-sensitive-to-nanoemulsive-chemodrugs
#13
Shan Liu, Dilong Chen, Yuming Yuan, Xue Zhang, Yao Li, Shenglei Yan, Jingqing Zhang
Evodiamine has been documented to possess activities in numerous cancer cells. Our preliminary study showed that A549 cells were insensitive to evodiamine. In this paper, A549 cells are sensitive to nanoemulsive evodiamine (EVONE) through an efficient intracellular and systematic delivery. EVONE entered tumor cells by energy-dependent and mainly through clathrin-mediated endocytosis. EVONE exerted a higher cytotoxicity in a dose- and time-dependent manner. The enhanced induction of cell cycle arrest was ascribed to the down-regulation of cyclin B and cyclin dependent kinase 1, while the enhanced induction of apoptosis was due to the activation of caspase -3, -8 and -9 and the decreased B-cell lymphoma 2/ assaciated X protein ratio...
April 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28472774/mitochondria-chaperone-grp75-moonlighting-as-a-cell-cycle-controller-to-derail-endocytosis-provides-an-opportunity-for-nanomicrosphere-intracellular-delivery
#14
Zhihui Gao, Xiuran Niu, Qing Zhang, Hang Chen, Aiai Gao, Shanshan Qi, Rong Xiang, Mattias Belting, Sihe Zhang
Understanding how cancer cells regulate endocytosis during the cell cycle could lead us to capitalize this event pharmacologically. Although certain endocytosis pathways are attenuated during mitosis, the endocytosis shift and regulation during the cell cycle have not been well clarified. The conventional concept of glucose-regulated proteins (GRPs) as protein folding chaperones was updated by discoveries that translocated GRPs assume moonlighting functions that modify the immune response, regulate viral release, and control intracellular trafficking...
April 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/28472690/nanoparticles-based-on-chitosan-hydrochloride-hyaluronic-acid-peg-containing-curcumin-in-vitro-evaluation-and-pharmacokinetics-in-rats
#15
Yurui Xu, Sajid Asghar, Liu Yang, Zhipeng Chen, Hongying Li, Wenwen Shi, Yibo Li, Qinqing Shi, Qineng Ping, Yanyu Xiao
Nanoparticles based on chitosan hydrochloride (CSH)-hyaluronic acid (HA)-PEG were prepared for delivering curcumin (CUR) (CUR-PNPs) to brain tumor. CUR-PNPs of 245.9nm and spherical morphology were obtained at optimized CSH/HA/PEG20000/CUR ratios with negative charge of about -27.2mV and EE of approximately 93.3%. Cytotoxicity studies showed that CUR-PNPs improved drug's anticancer activity in rat glioma cells (C6). The cellular uptake mechanism study showed active targeting of CUR-PNPs into C6 cells by HA mediated endocytosis...
May 1, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28472177/ubiquitin-carboxyl-terminal-esterase-l1-uchl1-is-associated-with-stem-like-cancer-cell-functions-in-pediatric-high-grade-glioma
#16
Patricia C Sanchez-Diaz, Judy C Chang, Emily S Moses, Tu Dao, Yidong Chen, Jaclyn Y Hung
Pediatric high-grade gliomas represent 8-12% of all primary tumors of the nervous system in children. Five-year survival for these pediatric aggressive tumors is poor (15-35%) indicating the need to develop better treatments for pediatric high-grade gliomas. In this work we used SF188 and SJ-GBM2 cell lines to study the function of the ubiquitin carboxyl-terminal esterase L1 (UCHL1), a deubiquitinase de-regulated in several cancers, in pediatric high-grade gliomas. UCHL1 depletion in SF188 and SJ-GBM2 glioma cells was associated with decreased cell proliferation and invasion, along with a reduced ability to grow in soft agar and to form spheres (i...
2017: PloS One
https://www.readbyqxmd.com/read/28468964/luminal-angiotensin-ii-is-internalized-as-a-complex-with-at1r-at2r-heterodimers-to-target-endoplasmic-reticulum-in-llc-pk1-cells
#17
Fernanda M Ferrão, Luiza H D Cardoso, Heather A Drummond, Xiao C Li, Jia L Zhuo, Dayene S Gomes, Lucienne S Lara, Adalberto Vieyra, Jennifer Lowe
Angiotensin II (ANG II) has many biological effects in renal physiology, particularly in Ca(2+) handling in the regulation of fluid and solute reabsorption. It involves the systemic endocrine renin-angiotensin system (RAS), but tissue and intracrine ANG II are also known. We have shown that ANG II induces heterodimerization of its AT1 and AT2 receptors (AT1R and AT2R) to stimulate sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) activity. Thus, we investigated whether ANG II-AT1R/AT2R complex is formed and internalized, and also looked at the intracellular localization of this complex to determine how its effect might be exerted on renal intracrine RAS...
May 3, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28466468/loss-of-dynamin-2-gtpase-function-results-in-microcytic-anaemia
#18
Fiona C Brown, Michael Collett, Cedric S Tremblay, Gerhard Rank, Pietro De Camilli, Carmen J Booth, Marc Bitoun, Phillip J Robinson, Benjamin T Kile, Stephen M Jane, David J Curtis
In a dominant mouse ethylnitrosurea mutagenesis screen for genes regulating erythropoiesis, we identified a pedigree with a novel microcytic hypochromia caused by a V235G missense mutation in Dynamin 2 (Dnm2). Mutations in Dnm2, a GTPase, are highly disease-specific and have been implicated in four forms of human diseases: centronuclear myopathy, Charcot-Marie Tooth neuropathy and, more recently, T-cell leukaemia and Hereditary Spastic Paraplegia, but red cell abnormalities have not been reported to date. The V235G mutation lies within a crucial GTP nucleotide-binding pocket of Dnm2, and resulted in defective GTPase activity and incompatibility with life in the homozygous state...
May 3, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28466393/improved-stability-and-enhanced-oral-bioavailability-of-atorvastatin-loaded-stearic-acid-modified-gelatin-nanoparticles
#19
Deepanshu Shilpi, Varun Kushwah, Ashish Kumar Agrawal, Sanyog Jain
PURPOSE: The present study evaluates the effects of stearic acid conjugation with gelatin and, its pharmaceutical potential to formulate novel atorvastatin (AT) loaded nanoparticles. METHOD: AT loaded stearic acid modified gelatin nanoparticles (AT-MG NPs) were prepared via two-step desolvation method with extensive optimization of different process variables. Further, the developed nanoparticles where evaluated against in vitro Caco-2 cell model and in vivo bioavailability...
May 2, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28465503/gene-expression-profiling-describes-the-genetic-regulation-of-meloidogyne-arenaria-resistance-in-arachis-hypogaea-and-reveals-a-candidate-gene-for-resistance
#20
Josh Clevenger, Ye Chu, Larissa Arrais Guimaraes, Thiago Maia, David Bertioli, Soraya Leal-Bertioli, Patricia Timper, C Corley Holbrook, Peggy Ozias-Akins
Resistance to root-knot nematode was introgressed into cultivated peanut Arachis hypogaea from a wild peanut relative, A. cardenasii and previously mapped to chromosome A09. The highly resistant recombinant inbred RIL 46 and moderately resistant RIL 48 were selected from a population with cv. Gregory (susceptible) and Tifguard (resistant) as female and male parents, respectively. RNA-seq analysis was performed on these four genotypes using root tissue harvested from root-knot nematode infected plants at 0, 3, 7 days after inoculation...
May 2, 2017: Scientific Reports
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