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clathrin endocytosis

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https://www.readbyqxmd.com/read/28734845/leveraging-siglec-8-endocytic-mechanisms-to-kill-human-eosinophils-and-malignant-mast-cells
#1
Jeremy A O'Sullivan, Daniela J Carroll, Yun Cao, Adriano N Salicru, Bruce S Bochner
BACKGROUND: Sialic acid-binding immunoglobulin-like lectin (Siglec)-8 is a cell-surface protein expressed selectively on human eosinophils, mast cells, and basophils, making it an ideal target for the treatment of diseases involving these cell types. However, the effective delivery of therapeutic agents to these cells requires an understanding of the dynamics of Siglec-8 surface expression. OBJECTIVE: To determine whether Siglec-8 is endocytosed in human eosinophils and malignant mast cells, identify mechanisms underlying its endocytosis, and demonstrate whether a toxin can be targeted to Siglec-8-bearing cells to kill these cells...
July 19, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28726952/porphyra-polysaccharide-derived-carbon-dots-for-non-viral-co-delivery-of-different-gene-combinations-and-neuronal-differentiation-of-ectodermal-mesenchymal-stem-cells
#2
Jiaxin Chen, Qiang Wang, Jie Zhou, Wenwen Deng, Qingtong Yu, Xia Cao, Jianping Wang, Fengxia Shao, Yang Li, Ping Ma, Myron Spector, Jiangnan Yu, Ximing Xu
In this study, multifunctional fluorescent carbon dots (CDs) were synthesized using a one-pot hydrothermal carbonization reaction, with the naturally-occurring porphyra polysaccharide (PPS) serving as a single carbon source for the first time and ethylenediamine (Ed) acting as the surface passivation agent. The resulting CDs enjoyed a high quantum yield (56.3%), excitation-dependent fluorescence, small size (<10 nm), spherical shape, uniform distribution, positive surface charge, low cytotoxicity and excellent ability to condense macromolecular plasmid DNA...
July 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28724764/rab5-and-rab11-are-required-for-clathrin-dependent-endocytosis-of-japanese-encephalitis-virus-in-bhk-21-cells
#3
Chun-Chun Liu, Yun-Na Zhang, Zhao-Yao Li, Jin-Xiu Hou, Jing Zhou, Lin Kan, Bin Zhou, Pu-Yan Chen
During infection Japanese Encephalitis Virus generally enters host cells via receptor-mediated clathrin dependent endocytosis. The trafficking of JEV within endosomes is controlled by Rab GTPases, but which Rab proteins are involved in JEV entry into BHK-21 cells is unknown. In this study, entry and post-internalization of JEV were analyzed using biochemical inhibitors, RNA interference and dominant negative mutants. Our data demonstrate that JEV entry into BHK-21 cells depends on clathrin, dynamin, and cholesterol, but not caveolae or macropinocytosis...
July 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28724758/the-shcd-phosphotyrosine-adaptor-subverts-canonical-egf-receptor-trafficking
#4
Melanie K B Wills, Hayley R Lau, Nina Jones
Shc family signalling adaptors connect activated transmembrane receptors to proximal effectors, and most also contain a sequence involved in clathrin-mediated receptor endocytosis. Notably, this AP2 adaptin binding motif (AD) is absent from the ShcD homolog, which also uniquely promotes ligand-independent phosphorylation of the Epidermal Growth Factor (EGF) Receptor (EGFR). We now report that cultured cells expressing ShcD exhibit reduced EGF uptake, commensurate with a decrease in EGFR surface presentation...
July 19, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28717225/a-mammalian-mirtron-mir-1224-promotes-tube-formation-of-human-primary-endothelial-cells-by-targeting-anti-angiogenic-factor-epsin2
#5
Eiko Sakai, Yusuke Miura, Emi Suzuki-Kouyama, Kengo Oka, Masashi Tachibana, Kenji Kawabata, Fuminori Sakurai, Hiroyuki Mizuguchi
Angiogenesis, new vessel formation from pre-existing vessels, is a highly conserved event through vertebrates. However, the system for tuning angiogenesis by species-intrinsic factors is totally unknown. miR-1224 is a member of mammal-specific mirtrons, which were identified as non-canonical microRNAs. We found that the expression of miR-1224 was upregulated in capillary-like tube-forming human umbilical vein endothelial cells on Matrigel. Enforced expression of miR-1224 stimulated tube formation, whereas repression of endogenous miR-1224 inhibited formation...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28706108/novel-function-of-a-dynein-light-chain-in-actin-assembly-during-clathrin-mediated-endocytosis
#6
Kristen B Farrell, Seth McDonald, Andrew K Lamb, Colette Worcester, Olve B Peersen, Santiago M Di Pietro
Clathrin- and actin-mediated endocytosis is essential in eukaryotic cells. In this study, we demonstrate that Tda2 is a novel protein of the endocytic machinery necessary for normal internalization of native cargo in yeast. Tda2 has not been classified in any protein family. Unexpectedly, solving the crystal structure of Tda2 revealed it belongs to the dynein light chain family. However, Tda2 works independently of the dynein motor complex and microtubules. Tda2 forms a tight complex with the polyproline motif-rich protein Aim21, which interacts physically with the SH3 domain of the Arp2/3 complex regulator Bbc1...
July 13, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28703125/flat-clathrin-lattices-are-dynamic-actin-controlled-hubs-for-clathrin-mediated-endocytosis-and-signalling-of-specific-receptors
#7
Daniela Leyton-Puig, Tadamoto Isogai, Elisabetta Argenzio, Bram van den Broek, Jeffrey Klarenbeek, Hans Janssen, Kees Jalink, Metello Innocenti
Clathrin lattices at the plasma membrane coat both invaginated and flat regions forming clathrin-coated pits and clathrin plaques, respectively. The function and regulation of clathrin-coated pits in endocytosis are well understood but clathrin plaques remain enigmatic nanodomains. Here we use super-resolution microscopy, molecular genetics and cell biology to show that clathrin plaques contain the machinery for clathrin-mediated endocytosis and cell adhesion, and associate with both clathrin-coated pits and filamentous actin...
July 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28701344/syp1-regulates-the-clathrin-mediated-and-clathrin-independent-endocytosis-of-multiple-cargo-proteins-through-a-novel-sorting-motif
#8
Amanda Reider Apel, Kyle Hoban, Silvia Chuartzman, Raffi Tonikian, Sachdev Sidhu, Maya Schuldiner, Beverly Wendland, Derek Prosser
Internalization of proteins from the plasma membrane (PM) allows for cell-surface composition regulation, signaling network modulation, and nutrient uptake. Clathrin-mediated endocytosis (CME) is a major internalization route for PM proteins. During CME, endocytic adaptor proteins bind cargos at the cell surface and link them to the PM and clathrin coat. Muniscins are a conserved family of endocytic adaptors, including Syp1 in budding yeast and its mammalian ortholog, FCHo1. These adaptors bind cargo via a C-terminal ì-homology domain (ìHD); however, few cargos exhibiting muniscin-dependent endocytosis have been identified, and the sorting sequence recognized by the μHD is unknown...
July 12, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28699138/toxicity-membrane-binding-and-uptake-of-the-sclerotinia-sclerotiorum-agglutinin-ssa-in-different-insect-cell-lines
#9
Ying Shen, Kristof De Schutter, Tomasz Walski, Els J M Van Damme, Guy Smagghe
The fungal lectin purified from Sclerotinia sclerotiorum, further referred to as Sclerotinia sclerotiorum agglutinin or SSA, possesses insecticidal activity against important pest insects such as pea aphids (Acyrthosiphon pisum). This paper aims at a better understanding of its activity at cellular level. Therefore, different insect cell lines were treated with SSA. These cell lines were derived from different tissues and represent the three major orders of insects important in agriculture: CF-203 (midgut Choristoneura fumiferana, Lepidoptera), GUTAW1 (midgut, Helicoverpa zea, Lepidoptera), High5 cells (ovary, Trichoplusia ni, Lepidoptera), Sf9 (ovary cells from Spodoptera frugiperda, Lepidoptera), S2 (hemocyte, Drosophila melanogaster, Diptera), and TcA (whole body, Tribolium castaneum, Coleoptera)...
July 11, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28697369/endocytosis-remote-control-from-deep-inside
#10
Ludger Johannes
The endoplasmic reticulum communicates directly through membrane contacts with other cellular organelles. A new study reports that the formation and detachment of endocytic uptake carriers for the clathrin-independent internalization of the epidermal growth factor receptor is dependent on such contact sites.
July 10, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28694083/impact-of-surface-functionalization-on-the-uptake-mechanism-and-toxicity-effects-of-silver-nanoparticles-in-hepg2-cells
#11
Lada Brkić Ahmed, Mirta Milić, Igor M Pongrac, Ana Marija Marjanović, Hrvoje Mlinarić, Ivan Pavičić, Srećko Gajović, Ivana Vinković Vrček
Safe and successful bioapplications of metallic nanoparticles depend on their physicochemical characteristics, in particular their surface properties. This study aimed to investigate how different surface functionalization of silver nanoparticles (AgNP) affect their interaction with mammalian liver cells with regard to cytotoxicity, genotoxicity and mechanism of cellular uptake. Differentially coated AgNP were prepared by surface functionalization using sodium bis(2-ethylhexyl)-sulfosuccinate (AOTAgNP), cetyltrimethylammonium bromide (CTABAgNP), poly(vinylpyrrolidone) (PVPAgNP), poly-l-lysine (PLLAgNP), and bovine serum albumin (BSAAgNP)...
July 8, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28687414/ovarian-expression-and-localization-of-clathrin-cltc-components-in-cutthroat-trout-oncorhynchus-clarki-evidence-for-cltc-involvement-in-endocytosis-of-vitellogenin-during-oocyte-growth
#12
Hiroko Mizuta, Yuji Mushirobira, Jun Nagata, Takashi Todo, Akihiko Hara, Benjamin J Reading, Craig V Sullivan, Naoshi Hiramatsu
To evaluate potential involvement of clathrin in endocytosis of vitellogenin (Vtg) by teleost oocytes, cDNAs encoding clathrin heavy chain (cltc) were cloned from ovaries of cutthroat trout. Quantitative PCR revealed three types of cltc (cltc-a1, cltc-a2, cltc-b) to be expressed in 10 different tissues including the ovary. The cltc-a1 alone exhibited a significant decrease in ovarian expression during vitellogenesis; this was correlated with a corresponding decrease in transcripts encoding the major Vtg receptor (Vtgr)...
July 4, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28687362/the-unexpected-function-of-a-highly-conserved-yxx%C3%AE-motif-in-hcv-core-protein
#13
Eirini Karamichali, Elisavet Serti, Aikaterini Gianneli, Aikaterini Papaefthymiou, Athanassios Kakkanas, Pelagia Foka, Alexandros Seremetakis, Konstantina Katsarou, Ioannis P Trougakos, Urania Georgopoulou
Hepatitis C virus (HCV) is an RNA positive strand virus, member of the Flaviviridae family. The HCV viral particle is composed of a capsid containing the genome, surrounded by an endoplasmic reticulum (ER)-derived lipid bilayer where E1 and E2 are assembled as heterodimers. However, different forms of viral particles have been identified in the serum of HCV-infected patients, including non-enveloped particles. Previous reports have demonstrated that HCV non-enveloped capsid-like particles (HCVne) can be generated by HCV core protein sequence...
July 5, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28686970/endocytic-pathways-of-optimized-resveratrol-cubosomes-capturing-into-human-hepatoma-cells
#14
Hend Mohamed Abdel-Bar, Rania Abd El Basset Sanad
Resveratrol (RSV) is a natural polyphenolic compound with high affinity to hepatocytes. It has numerous benefits as anticancer, antioxidant, immunomodulatory and cardioprotective actions. Nevertheless, RSV therapeutic applications are hindered by its low solubility, light sensitivity and extensive first-pass metabolism. Cubosomes are colloidally stable dispersed liquid crystalline nanoparticles. The incorporation of RSV into cubosomes could overcome some of its physicochemical limitations. A Design-Expert(®) software was applied to optimize cubosomes in terms of particle size and encapsulation efficiency (EE%)...
July 4, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28685122/nano-engineered-skin-mesenchymal-stem-cells-potential-vehicles-for-tumour-targeted-quantum-dot-delivery
#15
Liga Saulite, Dominyka Dapkute, Karlis Pleiko, Ineta Popena, Simona Steponkiene, Ricardas Rotomskis, Una Riekstina
Nanotechnology-based drug design offers new possibilities for the use of nanoparticles in imaging and targeted therapy of tumours. Due to their tumour-homing ability, nano-engineered mesenchymal stem cells (MSCs) could be utilized as vectors to deliver diagnostic and therapeutic nanoparticles into a tumour. In the present study, uptake and functional effects of carboxyl-coated quantum dots QD655 were studied in human skin MSCs. The effect of QD on MSCs was examined using a cell viability assay, Ki67 expression analysis, and tri-lineage differentiation assay...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28684042/signal-dependent-transport-of-a-membrane-cargo-from-early-endosomes-to-recycling-endosomes
#16
Ismail S Mahmoud, Jade Louber, Steve K Dower, Anne M Verhagen, Paul A Gleeson
Many membrane cargoes undergo endocytosis and intracellular recycling to the plasma membrane via the early endosomes and the recycling endosomes. However whether specific sorting signals are required for transport from early endosomes to recycling endosomes is not known and the current view is that transport to the recycling endosomes is by a passive default process. Here we show that the cytoplasmic tail of the neonatal Fc receptor (FcRn) contains discrete signals for endocytosis and for sorting to the recycling endosomes...
June 17, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28676702/extracellular-matrix-promotes-clathrin-dependent-endocytosis-of-prolactin-and-stat5-activation-in-differentiating-mammary-epithelial-cells
#17
Rebecca E Bridgewater, Charles H Streuli, Patrick T Caswell
The hormone prolactin promotes lactational differentiation of mammary epithelial cells (MECs) via its cognate receptor and the downstream JAK2-STAT5a signalling pathway. In turn this regulates transcription of milk protein genes. Prolactin signalling depends on a cross-talk with basement membrane extracellular matrix (ECM) via β1 integrins which activate both ILK and Rac1 and are required for STAT5a activation and lactational differentiation. Endocytosis is an important regulator of signalling. It can both enhance and suppress cytokine signalling, although the role of endocytosis for prolactin signalling is not known...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28674529/abl-tyrosine-kinase-regulates-hepatitis-c-virus-entry
#18
Saehong Min, Yun-Sook Lim, Dongjo Shin, Chorong Park, Jae-Bong Park, Seungtaek Kim, Marc P Windisch, Soon B Hwang
Abl is a central regulator of multiple cellular processes controlling actin dynamics, proliferation, and differentiation. Here, we showed that knockdown of Abl impaired hepatitis C virus (HCV) propagation. Treatment of Abl tyrosine kinase-specific inhibitor, imatinib and dasatinib, also significantly decreased HCV RNA and protein levels in HCV-infected cells. We showed that both imatinib and dasatinib selectively inhibited HCV infection at the entry step of HCV life cycle, suggesting that Abl kinase activity may be necessary for HCV entry...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28674108/reciprocal-regulation-of-signaling-and-endocytosis-implications-for-the-evolving-cancer-cell
#19
REVIEW
Sandra L Schmid
Cell surface receptor uptake via clathrin-mediated endocytosis (CME) and subsequent intracellular sorting for degradation or recycling regulates the strength and specificity of downstream signaling. Signaling, in turn, modulates early endocytic trafficking. This reciprocal regulation of signaling and endocytosis provides opportunities for the establishment of feedback loops to enhance or suppress surface-derived signals. Recent studies suggest that dynamin-1, a presumed neuron-specific isoform of the large, membrane fission GTPase, can be activated in nonneuronal cells downstream of cancer-relevant signaling pathways and thereby function as a nexus between signaling and early endocytic trafficking...
July 3, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28674075/clathrin-mediated-post-fusion-membrane-retrieval-influences-the-exocytic-mode-of-endothelial-weibel-palade-bodies
#20
Nicola L Stevenson, Ian J White, Jessica J McCormack, Christopher Robinson, Daniel F Cutler, Thomas D Nightingale
Weibel Palade bodies (WPB), the storage organelles of endothelial cells, are essential to normal haemostatic and inflammatory responses. Their major content protein is Von Willebrands factor (VWF) that, following secretagogue-stimulation, is released into the blood vessel lumen as large platelet-catching strings. This exocytosis changes the protein composition of the cell surface and also results in a net increase in the amount of plasma membrane. Compensatory endocytosis is thought to limit changes in cell size and retrieve fusion machinery and other misplaced integral membrane proteins following exocytosis, however little is known about the extent, timing, mechanism and precise function of compensatory endocytosis in endothelial cells...
July 3, 2017: Journal of Cell Science
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