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clathrin endocytosis

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https://www.readbyqxmd.com/read/29241687/mechanisms-of-carrier-formation-during-clathrin-independent-endocytosis
#1
REVIEW
Antonio P A Ferreira, Emmanuel Boucrot
Clathrin-independent endocytosis (CIE) mediates the cellular uptake of many extracellular ligands, receptors, and pathogens, including several life-threatening bacterial toxins and viruses. So far, our understanding of CIE carrier formation has lagged behind that of clathrin-coated vesicles. Impediments have been the imprecise definition of some CIE pathways, the lack of specific cargoes being transported and of exclusive cytosolic markers and regulators. Notwithstanding these limitations, three distinct molecular mechanisms by which CIE carriers form can be defined...
December 11, 2017: Trends in Cell Biology
https://www.readbyqxmd.com/read/29236694/dynamics-of-phosphoinositide-conversion-in-clathrin-mediated-endocytic-traffic
#2
Kangmin He, Robert Marsland Iii, Srigokul Upadhyayula, Eli Song, Song Dang, Benjamin R Capraro, Weiming Wang, Wesley Skillern, Raphael Gaudin, Minghe Ma, Tom Kirchhausen
Vesicular carriers transport proteins and lipids from one organelle to another, recognizing specific identifiers for the donor and acceptor membranes. Two important identifiers are phosphoinositides and GTP-bound GTPases, which provide well-defined but mutable labels. Phosphatidylinositol and its phosphorylated derivatives are present on the cytosolic faces of most cellular membranes. Reversible phosphorylation of its headgroup produces seven distinct phosphoinositides. In endocytic traffic, phosphatidylinositol-4,5-biphosphate marks the plasma membrane, and phosphatidylinositol-3-phosphate and phosphatidylinositol-4-phosphate mark distinct endosomal compartments...
December 13, 2017: Nature
https://www.readbyqxmd.com/read/29212877/high-speed-super-resolution-imaging-of-the-proteins-in-fission-yeast-clathrin-mediated-endocytic-actin-patches
#3
Rajesh Arasada, Wasim A Sayyad, Julien Berro, Thomas D Pollard
To internalize nutrients and cell surface receptors via clathrin mediated endocytosis cells assemble at least 50 proteins including clathrin, clathrin-interacting proteins, actin filaments and actin binding proteins in a highly ordered and regulated fashion. The molecular mechanism by which actin filament polymerization deforms the cell membrane is unknown, largely due to lack of knowledge about the organization of the regulatory proteins and actin filaments. We used high-speed super-resolution localization microscopy (FPALM) of live fission yeast cells to improve the spatial resolution to ∼35 nm with 1 s temporal resolution...
December 6, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29203863/orai-channels-are-critical-for-receptor-mediated-endocytosis-of-albumin
#4
Bo Zeng, Gui-Lan Chen, Eliana Garcia-Vaz, Sunil Bhandari, Nikoleta Daskoulidou, Lisa M Berglund, Hongni Jiang, Thomas Hallett, Lu-Ping Zhou, Li Huang, Zi-Hao Xu, Viji Nair, Robert G Nelson, Wenjun Ju, Matthias Kretzler, Stephen L Atkin, Maria F Gomez, Shang-Zhong Xu
Impaired albumin reabsorption by proximal tubular epithelial cells (PTECs) has been highlighted in diabetic nephropathy (DN), but little is known about the underlying molecular mechanisms. Here we find that ORAI1-3, are preferentially expressed in PTECs and downregulated in patients with DN. Hyperglycemia or blockade of insulin signaling reduces the expression of ORAI1-3. Inhibition of ORAI channels by BTP2 and diethylstilbestrol or silencing of ORAI expression impairs albumin uptake. Transgenic mice expressing a dominant-negative Orai1 mutant (E108Q) increases albuminuria, and in vivo injection of BTP2 exacerbates albuminuria in streptozotocin-induced and Akita diabetic mice...
December 4, 2017: Nature Communications
https://www.readbyqxmd.com/read/29193748/modulation-of-cell-responses-to-ag-meo2-ma-co-oegma-effects-of-nanoparticle-surface-hydrophobicity-and-serum-proteins-on-cellular-uptake-and-toxicity
#5
Christopher R Anderson, Yehou D M Gnopo, Filippo Gambinossi, Steven E Mylon, James K Ferri
Nanoparticle (NP) interactions with cellular systems are influenced by both NP physico-chemical properties and the presence of surface-bound proteins that are adsorbed in biological environments. Here, we characterize cellular responses to silver nanoparticles (AgNPs) functionalized with poly(MeO2 MAx -co-OEGMAy ) brushes with tunable hydrophobicity and explore how these responses are modulated by the presence or absence of serum proteins. Poly(MeO2 MAx -co-OEGMAy ) with variable composition (5-10% OEGMA) was fabricated to elicit differential hydrophobicity at 37°C for AgNPs capped with these copolymers...
November 30, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29192062/distinct-roles-for-plasma-membrane-ptdins-4-p-and-ptdins-4-5-p2-during-yeast-receptor-mediated-endocytosis
#6
Wataru Yamamoto, Suguru Wada, Makoto Nagano, Kaito Aoshima, Daria Elisabeth Siekhaus, Junko Y Toshima, Jiro Toshima
Clathrin-mediated endocytosis requires the coordinated assembly of various endocytic proteins and lipids at the plasma membrane. Accumulating evidence demonstrates a crucial role for phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) in endocytosis, but specific roles for PtdIns(4)P other than as the biosynthetic precursor of PtdIns(4,5)P2 have not been clarified. In this study we investigated the role of PtdIns(4)P or PtdIns(4,5)P2 in receptor-mediated endocytosis through the construction of temperature-sensitive (ts) mutants for the PI 4-kinases Stt4p and Pik1p and the PtdIns(4) 5-kinase Mss4p...
November 30, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/29187674/nitrative-dna-damage-in-cultured-macrophages-exposed-to-indium-oxide
#7
Tahmina Afroz, Yusuke Hiraku, Ning Ma, Sharif Ahmed, Shinji Oikawa, Shosuke Kawanishi, Mariko Murata
OBJECTIVES: Indium compounds are used in manufacturing displays of mobile phones and televisions. However, these materials cause interstitial pneumonia in exposed workers. Animal experiments demonstrated that indium compounds caused lung cancer. Chronic inflammation is considered to play a role in lung carcinogenesis and fibrosis induced by particulate matters. 8-Nitroguanine (8-nitroG) is a mutagenic DNA lesion formed during inflammation and may participate in carcinogenesis. To clarify the mechanism of carcinogenesis, we examined 8-nitroG formation in indium-exposed cultured cells...
November 29, 2017: Journal of Occupational Health
https://www.readbyqxmd.com/read/29186283/intracellular-trafficking-of-transforming-growth-factor-%C3%AE-receptors
#8
Ihor Yakymovych, Mariya Yakymovych, Carl-Henrik Heldin
Transforming growth factor β (TGFβ) family members signal via heterotetrameric complexes of type I (TβRI) and type II (TβRII) dual specificity kinase receptors. The availability of the receptors on the cell surface is controlled by several mechanisms. Newly synthesized TβRI and TβRII are delivered from the Golgi apparatus to the cell surface via separate routes. On the cell surface, TGFβ receptors are distributed between different microdomains of the plasma membrane and can be internalized via clathrin- and caveolae-mediated endocytic mechanisms...
November 25, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29186191/lipopolysaccharide-structure-impacts-the-entry-kinetics-of-bacterial-outer-membrane-vesicles-into-host-cells
#9
Eloise J O'Donoghue, Natalie Sirisaengtaksin, Douglas F Browning, Ewa Bielska, Mohammed Hadis, Francisco Fernandez-Trillo, Luke Alderwick, Sara Jabbari, Anne Marie Krachler
Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis. However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells. Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe...
November 29, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29184887/weak-molecular-interactions-in-clathrin-mediated-endocytosis
#10
REVIEW
Sarah M Smith, Michael Baker, Mary Halebian, Corinne J Smith
Clathrin-mediated endocytosis is a process by which specific molecules are internalized from the cell periphery for delivery to early endosomes. The key stages in this step-wise process, from the starting point of cargo recognition, to the later stage of assembly of the clathrin coat, are dependent on weak interactions between a large network of proteins. This review discusses the structural and functional data that have improved our knowledge and understanding of the main weak molecular interactions implicated in clathrin-mediated endocytosis, with a particular focus on the two key proteins: AP2 and clathrin...
2017: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/29174139/altered-tgf-%C3%AE-endocytic-trafficking-contributes-to-the-increased-signaling-in-marfan-syndrome
#11
Anna-Maria Siegert, Carla Serra-Peinado, Enric Gutiérrez-Martínez, Fernando Rodríguez-Pascual, Isabel Fabregat, Gustavo Egea
The main cardiovascular alteration in Marfan syndrome (MFS) is the formation of aortic aneurysms in which augmented TGF-β signaling is reported. However, the primary role of TGF-β signaling as a molecular link between the genetic mutation of fibrillin-1 and disease onset is controversial. The compartmentalization of TGF-β endocytic trafficking has been shown to determine a signaling response in which clathrin-dependent internalization leads to TGF-β signal propagation, and caveolin-1 (CAV-1) associated internalization leads to signal abrogation...
November 23, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29173029/enzymatically-synthesized-megalo-type-isomaltosaccharides-enhance-the-barrier-function-of-the-tight-junction-in-the-intestinal-epithelium
#12
Hiroshi Hara, Shunsuke Kume, Takahisa Iizuka, Yoshinori Fujimoto, Atsuo Kimura
Megalo-type isomaltosaccharides are an enzymatically synthesized foodstuff produced by transglucosylation from maltodextrin, and they contain a mid-chain length polymer of D-glucose with α-1,6-glycoside linkages. The injection of a solution of megalo-type isomaltosaccharides (1-4%(w/v), average DP = 12.6), but not oligo-type isomaltosaccharides (average DP = 3.3), into the intestinal lumen dose-dependently reduced the transport rates of tight junction permeable markers in a ligated loop of the anesthetized rat jejunum...
November 27, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29167320/mapks-influence-pollen-tube-growth-by-controlling-the-formation-of-phosphatidylinositol-4-5-bisphosphate-in-an-apical-plasma-membrane-domain
#13
Franziska Hempel, Irene Stenzel, Mareike Heilmann, Praveen Krishnamoorthy, Wilhelm Menzel, Ralph Golbik, Stefan Helm, Dirk Dobritzsch, Sacha Baginsky, Justin Lee, Wolfgang Hoehenwarter, Ingo Heilmann
An apical plasma membrane domain enriched in the regulatory phospholipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) is critical for polar tip growth of pollen tubes. How the biosynthesis of PtdIns(4,5)P2 by phosphatidylinositol 4-phosphate 5-kinases (PI4P 5-kinases) is controlled by upstream signaling is currently unknown. The pollen-expressed PI4P 5-kinase PIP5K6 is required for clathrin-mediated endocytosis and polar tip growth in pollen tubes. Here, we identify PIP5K6 as a target of the pollen-expressed mitogen-activated protein kinase MPK6 and characterize the regulatory effects...
November 22, 2017: Plant Cell
https://www.readbyqxmd.com/read/29166788/histopathological-indices-and-inflammatory-response-in-the-digestive-gland-of-the-mussel-mytilus-galloprovincialis-as-biomarker-of-immunotoxicity-to-silver-nanoparticles
#14
Younes Bouallegui, Ridha Ben Younes, Houda Bellamine, Ridha Oueslati
BACKGROUND: Histopathological assessments approaches in bivalves have become an important tool in environmental toxicology. This study seeks to develop a quantitative histopathological index (Ih) and inflammation score as biomarkers in the aim to assess the health status of nanoparticles exposed mussels. METHODS: Digestive gland Hematoxylin and eosin (H&E) stained sections from Mytilus galloprovincialis were assessed after in vivo exposure (for 3, 6 and 12h) to silver nanoparticles (Ag-NPs < 50nm and Ag-NPs < 100nm) prior and after manipulating the potential uptake pathways (clathrin- and caveolae-mediated endocytosis) using amantadine and nystatin as blockers...
November 23, 2017: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/29162374/the-efficiency-and-mechanism-of-n-octyl-o-n-carboxymethyl-chitosan-based-micelles-to-enhance-the-oral-absorption-of-silybin
#15
Tingjie Yin, Ying Zhang, Yanhong Liu, Qinyu Chen, Ying Fu, Jinlai Liang, Jianping Zhou, Xiaomeng Tang, Jiyong Liu, Meirong Huo
This study demonstrates the preparation of a silybin-loaded N-octyl-O, N-carboxymethyl chitosan micelle (OCC-SLB) to enhance the oral absorption efficiency of silybin (SLB) and investigate the related mechanisms of enhancement. Firstly, the physicochemical properties of OCC and OCC-SLB micelles, including critical micelle concentration (CMC), particle size, zeta potential, drug-loading, etc., were determined. Results of pharmacokinetic studies on rats then confirmed a desirable enhancement in the oral bioavailability of SLB by OCC-SLB micelles compared with a stock SLB suspension solution...
November 18, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29161595/a-coincidence-detection-mechanism-controls-px-bar-domain-mediated-endocytic-membrane-remodeling-via-an-allosteric-structural-switch
#16
Wen-Ting Lo, Andreja Vujičić Žagar, Fabian Gerth, Martin Lehmann, Dymtro Puchkov, Oxana Krylova, Christian Freund, Leonardo Scapozza, Oscar Vadas, Volker Haucke
Clathrin-mediated endocytosis occurs by bending and remodeling of the membrane underneath the coat. Bin-amphiphysin-rvs (BAR) domain proteins are crucial for endocytic membrane remodeling, but how their activity is spatiotemporally controlled is largely unknown. We demonstrate that the membrane remodeling activity of sorting nexin 9 (SNX9), a late-acting endocytic PX-BAR domain protein required for constriction of U-shaped endocytic intermediates, is controlled by an allosteric structural switch involving coincident detection of the clathrin adaptor AP2 and phosphatidylinositol-3,4-bisphosphate (PI(3,4)P2) at endocytic sites...
November 20, 2017: Developmental Cell
https://www.readbyqxmd.com/read/29161594/clathrin-assembly-defines-the-onset-and-geometry-of-cortical-patterning
#17
Yang Yang, Ding Xiong, Anne Pipathsouk, Orion D Weiner, Min Wu
Assembly of the endocytic machinery is a constitutively active process that is important for the organization of the plasma membrane, signal transduction, and membrane trafficking. Existing research has focused on the stochastic nature of endocytosis. Here, we report the emergence of the collective dynamics of endocytic proteins as periodic traveling waves on the cell surface. Coordinated clathrin assembly provides the earliest spatial cue for cortical waves and sets the direction of propagation. Surprisingly, the onset of clathrin waves, but not individual endocytic events, requires feedback from downstream factors, including FBP17, Cdc42, and N-WASP...
November 20, 2017: Developmental Cell
https://www.readbyqxmd.com/read/29158566/graphene-quantum-dots-for-cell-proliferation-nucleus-imaging-and-photoluminescent-sensing-applications
#18
Mukesh Kumar Kumawat, Mukeshchand Thakur, Raju B Gurung, Rohit Srivastava
We report a simple one-pot microwave assisted "green synthesis" of Graphene Quantum Dots (GQDs) using grape seed extract as a green therapeutic carbon source. These GQDs readily self-assemble, hereafter referred to as "self-assembled" GQDs (sGQDs) in the aqueous medium. The sGQDs enter via caveolae and clathrin-mediated endocytosis and target themselves into cell nucleus within 6-8 h without additional assistance of external capping/targeting agent. The tendency to self-localize themselves into cell nucleus also remains consistent in different cell lines such as L929, HT-1080, MIA PaCa-2, HeLa, and MG-63 cells, thereby serving as a nucleus labelling agent...
November 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150658/differential-regulation-of-synaptic-ap-2-clathrin-vesicle-uncoating-in-synaptic-plasticity
#19
Ermes Candiello, Ratnakar Mishra, Bernhard Schmidt, Olaf Jahn, Peter Schu
AP-1/σ1B-deficiency causes X-linked intellectual disability. AP-1/σ1B -/- mice have impaired synaptic vesicle recycling, fewer synaptic vesicles and enhanced endosome maturation mediated by AP-1/σ1A. Despite defects in synaptic vesicle recycling synapses contain two times more endocytic AP-2 clathrin-coated vesicles. We demonstrate increased formation of two classes of AP-2/clathrin coated vesicles. One which uncoats readily and a second with a stabilised clathrin coat. Coat stabilisation is mediated by three molecular mechanisms: reduced recruitment of Hsc70 and synaptojanin1 and enhanced μ2/AP-2 phosphorylation and activation...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29145001/in-vitro-and-in-vivo-evaluation-of-10-hydroxycamptothecin-loaded-poly-n-butyl-cyanoacrylate-nanoparticles-prepared-by-miniemulsion-polymerization
#20
Xin Jin, Sajid Asghar, Xieting Zhu, Zhipeng Chen, Cihui Tian, Lining Yin, Qineng Ping, Yanyu Xiao
In this paper, 10-hydroxycamptothecin (HCPT)-loaded poly (n-butyl cyanoacrylate) nanoparticles (HCPT-PBCA-NPs) co-modified with polysorbate 80, soybean phospholipids, and polyethylene glycol (100) monostearate were successfully prepared via miniemulsion polymerization, and were characterized for particle size, morphology, zeta potential, encapsulation efficiency (EE) and drug loading capacity (DL). The chemical structure of HCPT-PBCA-NPs and the state of HCPT in the PBCA-NPs were investigated by DSC, FTIR and (1)H NMR...
November 12, 2017: Colloids and Surfaces. B, Biointerfaces
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