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clathrin endocytosis

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https://www.readbyqxmd.com/read/28638979/endocytosis-is-required-for-exocytosis-and-priming-of-respiratory-burst-activity-in-human-neutrophils
#1
T Michael Creed, Shweta Tandon, Richard A Ward, Kenneth R McLeish
OBJECTIVE AND DESIGN: Neutrophil generation of reactive oxygen species (ROS) is enhanced by exposure to pro-inflammatory agents in a process termed priming. Priming is depending on exocytosis of neutrophil granules and p47(phox) phosphorylation-dependent translocation of cytosolic NADPH oxidase components. Clathrin-mediated endocytosis was recently reported to be necessary for priming, but the mechanism linking endocytosis to priming was not identified. The present study examined the hypothesis that endocytosis regulates neutrophil priming by controlling granule exocytosis...
June 21, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/28634871/rabies-virus-co-localizes-with-early-rab5-and-late-rab7-endosomal-proteins-in-neuronal-and-sh-sy5y-cells
#2
Waqas Ahmad, Yingying Li, Yidi Guo, Xinyu Wang, Ming Duan, Zhenhong Guan, Zengshan Liu, Maolin Zhang
Rabies virus (RABV) is a highly neurotropic virus that follows clathrin-mediated endocytosis and pH-dependent pathway for trafficking and invasion into endothelial cells. Early (Rab5, EEA1) and late (Rab7, LAMP1) endosomal proteins play critical roles in endosomal sorting, maturity and targeting various molecular cargoes, but their precise functions in the early stage of RABV neuronal infection remain elusive. In this study, the relationship between enigmatic entry of RABV with these endosomal proteins into neuronal and SH-SY5Y cells was investigated...
June 16, 2017: Virologica Sinica
https://www.readbyqxmd.com/read/28629179/epidermal-growth-factor-enhances-cellular-uptake-of-polystyrene-nanoparticles-by-clathrin-mediated-endocytosis
#3
Le Thi Minh Phuc, Akiyoshi Taniguchi
The interaction between nanoparticles and cells has been studied extensively, but most research has focused on the effect of various nanoparticle characteristics, such as size, morphology, and surface charge, on the cellular uptake of nanoparticles. In contrast, there have been very few studies to assess the influence of cellular factors, such as growth factor responses, on the cellular uptake efficiency of nanoparticles. The aim of this study was to clarify the effects of epidermal growth factor (EGF) on the uptake efficiency of polystyrene nanoparticles (PS NPs) by A431 cells, a human carcinoma epithelial cell line...
June 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28627515/lipid-mediated-px-bar-domain-recruitment-couples-local-membrane-constriction-to-endocytic-vesicle-fission
#4
Johannes Schöneberg, Martin Lehmann, Alexander Ullrich, York Posor, Wen-Ting Lo, Gregor Lichtner, Jan Schmoranzer, Volker Haucke, Frank Noé
Clathrin-mediated endocytosis (CME) involves membrane-associated scaffolds of the bin-amphiphysin-rvs (BAR) domain protein family as well as the GTPase dynamin, and is accompanied and perhaps triggered by changes in local lipid composition. How protein recruitment, scaffold assembly and membrane deformation is spatiotemporally controlled and coupled to fission is poorly understood. We show by computational modelling and super-resolution imaging that phosphatidylinositol 3,4-bisphosphate [PI(3,4)P2] synthesis within the clathrin-coated area of endocytic intermediates triggers selective recruitment of the PX-BAR domain protein SNX9, as a result of complex interactions of endocytic proteins competing for phospholipids...
June 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28619886/tubular-clathrin-ap-2-lattices-pinch-collagen-fibers-to-support-3d-cell-migration
#5
Nadia Elkhatib, Enzo Bresteau, Francesco Baschieri, Alba López Rioja, Guillaume van Niel, Stéphane Vassilopoulos, Guillaume Montagnac
Migrating cells often use focal adhesions in order to move. Focal adhesions are less prominent in cells migrating in three-dimensional (3D) as compared with 2D environments. We looked for alternative adhesion structures supporting cell migration. We analyzed the dynamics of clathrin-coated pits in cells migrating in a 3D environment of collagen fibers. Both topological cues and local engagement of integrins triggered the accumulation of clathrin-coated structures on fibers. Clathrin/adaptor protein 2 (AP-2) lattices pinched collagen fibers by adopting a tube-like morphology and regulated adhesion to fibers in an endocytosis-independent manner...
June 16, 2017: Science
https://www.readbyqxmd.com/read/28618637/actin-growth-profile-in-clathrin-mediated-endocytosis
#6
D J Tweten, P V Bayly, A E Carlsson
Clathrin-mediated endocytosis in yeast is driven by a protein patch containing close to 100 different types of proteins. Among the proteins are 5000-10000 copies of polymerized actin, and successful endocytosis requires growth of the actin network. Since it is not known exactly how actin network growth drives endocytosis, we calculate the spatial distribution of actin growth required to generate the force that drives the process. First, we establish the force distribution that must be supplied by actin growth, by combining membrane-bending profiles obtained via electron microscopy with established theories of membrane mechanics...
May 2017: Physical Review. E
https://www.readbyqxmd.com/read/28615320/differential-phosphorylation-signals-control-endocytosis-of-gpr15
#7
Yukari Okamoto, Sojin Shikano
GPR15 is an orphan G protein-coupled receptor (GPCR) that serves for an HIV coreceptor and was also recently found as a novel homing receptor for T cells implicated in colitis. We show that GPR15 undergoes a constitutive endocytosis in the absence of ligand. The endocytosis was clathrin-dependent and partially dependent on β-arrestin in HEK293 cells, and nearly half of the internalized GPR15 receptors were recycled to the plasma membrane. Ala mutation of the distal C-terminal Arg(354) or Ser(357), which forms a consensus phosphorylation site for basophilic kinases, markedly reduced the endocytosis while phosphomimetic mutation of Ser(357) to Asp did not...
June 14, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28615098/-human-ev71-invades-human-neuroblastoma-sk-n-sh-cells-by-clathrin-mediated-endocytosis
#8
Yanhua Xu, Quanbo Liu, Zhenzhen Zhang
Objective To study the mechanism ofhuman enterovirus 71 (EV71) entering human neuroblastoma SK-N-SH cells. Methods After the SK-N-SH cells were pretreated with chlorpromazine (CPZ) or nystatin (NT), real-time quantitative PCR (qRT-PCR) was employed to measure EV71 mRNA level, and indirect immunofluorescence microscopy was used to detect the expression level of viral protein 1 (VP1) in the target cells. In order to reveal the colocalization of EV71 with clathrin, laser confocal microscopy was performed on the infected cells...
June 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28607034/tctex-1-controls-ciliary-resorption-by-regulating-branched-actin-polymerization-and-endocytosis
#9
Masaki Saito, Wataru Otsu, Kuo-Shun Hsu, Jen-Zen Chuang, Teruyuki Yanagisawa, Vincent Shieh, Taku Kaitsuka, Fan-Yan Wei, Kazuhito Tomizawa, Ching-Hwa Sung
The primary cilium is a plasma membrane-protruding sensory organelle that undergoes regulated assembly and resorption. While the assembly process has been studied extensively, the cellular machinery that governs ciliary resorption is less well understood. Previous studies showed that the ciliary pocket membrane is an actin-rich, endocytosis-active periciliary subdomain. Furthermore, Tctex-1, originally identified as a cytoplasmic dynein light chain, has a dynein-independent role in ciliary resorption upon phosphorylation at Thr94...
June 12, 2017: EMBO Reports
https://www.readbyqxmd.com/read/28604134/impact-of-exposure-time-particle-size-and-uptake-pathway-on-silver-nanoparticle-effects-on-circulating-immune-cells-in-mytilus-galloprovincialis
#10
Younes Bouallegui, Ridha Ben Younes, Faten Turki, Ridha Oueslati
Nanomaterials have increasingly emerged as potential pollutants to aquatic organisms. Nanomaterials are known to be taken up by hemocytes of marine invertebrates including Mytilus galloprovincialis. Indeed, assessments of hemocyte-related parameters are a valuable tool in the determination of potentials for nanoparticle (NP) toxicity. The present study assessed the effects from two size types of silver nanoparticles (AgNP: <50 nm and <100 nm) on the frequency of hemocytes subpopulations as immunomodulation biomarkers exposed in a mollusk host...
December 2017: Journal of Immunotoxicology
https://www.readbyqxmd.com/read/28603974/evolution-of-new-bolaliposomes-using-novel-%C3%AF-tocopheryl-succinate-based-cationic-lipid-and-1-12-disubstituted-dodecane-based-bolaamphiphile-for-efficient-gene-delivery
#11
Mallikarjun Gosangi, Hithavani Rapaka, Venkatesh Ravula, Srilakshmi Venkata Patri
Non-viral lipid based vectors are promising transporting systems for the intracellular delivery of therapeutic gene sequences and directly influence the success of gene delivery. However, the associated drawbacks like lower transfection, toxicity and targetability with which demands further improvisation. Towards this goal, herein, we report a novel lipid formulation by mixing two distinct cationic surfactants such as tocopheryl succinate based cationic lipid and 1, 12 dodecane based bolaamphiphile and is proved as good transfection reagent with its competing potential with "golden" standard, Lipofectamine 3000 (L3K)...
June 12, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28592413/recruitment-of-endosomal-signaling-mediates-the-forskolin-modulation-of-guinea-pig-cardiac-neuron-excitability
#12
Jean C Hardwick, Todd A Clason, John D Tompkins, Beatrice M Girard, Caitlin N Baran, Laura A Merriam, Victor May, Rodney L Parsons
Forskolin, a selective activator of adenylyl cyclase (AC), commonly is used to establish actions of G protein coupled receptors (GPCRs) that are initiated primarily through activation of AC/cAMP signaling pathways. In the present study, forskolin was used to evaluate the potential role of AC/cAMP, which is a major signaling mechanism for the pituitary adenylate cyclase-activating polypeptide (PACAP)-selective PAC1 receptor, in the regulation of guinea pig cardiac neuronal excitability. Forskolin (5-10 µM) increases excitability in ~60 % of the cardiac neurons...
June 7, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28584192/arf6-and-rab22-mediate-t-cell-conjugate-formation-by-regulating-clathrin-independent-endosomal-membrane-trafficking
#13
Debra L Johnson, Jessica Wayt, Jean M Wilson, Julie G Donaldson
Endosomal trafficking can influence the composition of the plasma membrane and the ability of cells to polarize their membranes. Here we examined whether trafficking through clathrin-independent endocytosis (CIE) affects the ability of T cells to form a cell-cell conjugate with antigen presenting cells (APCs). We show that CIE occurs in both the Jurkat T cell line and primary human T cells. In Jurkat cells, the activities of two guanine nucleotide binding proteins, Arf6 and Rab22, influence CIE and conjugate formation...
June 5, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28583008/effect-of-exposure-time-particle-size-and-uptake-pathways-in-immune-cell-lysosomal-cytotoxicity-of-mussels-exposed-to-silver-nanoparticles
#14
Younes Bouallegui, Ridha Ben Younes, Faten Turki, Amine Mezni, Ridha Oueslati
Cytotoxicity evaluation of hemocytes (lysosomal membrane stability [LMS] assay) from Mytilus galloprovincialis Lamarck, exposed to a sublethal dose (100 μg/L) of two size of silver nanoparticles (AgNPs: <50 nm and <100 nm) - prior to and after inhibition of potential uptake pathways (i.e., clathrin- and caveolae-mediated endocytosis) within different times of exposure (3, 6, 12 h) - showed that there was a significant cytotoxic effect on immune cells of mussels exposed for different times to either AgNP size (p < 0...
June 6, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28581510/nanoscale-manipulation-of-membrane-curvature-for-probing-endocytosis-in-live-cells
#15
Wenting Zhao, Lindsey Hanson, Hsin-Ya Lou, Matthew Akamatsu, Praveen D Chowdary, Francesca Santoro, Jessica R Marks, Alexandre Grassart, David G Drubin, Yi Cui, Bianxiao Cui
Clathrin-mediated endocytosis (CME) involves nanoscale bending and inward budding of the plasma membrane, by which cells regulate both the distribution of membrane proteins and the entry of extracellular species. Extensive studies have shown that CME proteins actively modulate the plasma membrane curvature. However, the reciprocal regulation of how the plasma membrane curvature affects the activities of endocytic proteins is much less explored, despite studies suggesting that membrane curvature itself can trigger biochemical reactions...
June 5, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28579939/the-role-of-co-chaperones-in-synaptic-proteostasis-and-neurodegenerative-disease
#16
REVIEW
Erica L Gorenberg, Sreeganga S Chandra
Synapses must be preserved throughout an organism's lifespan to allow for normal brain function and behavior. Synapse maintenance is challenging given the long distances between the termini and the cell body, reliance on axonal transport for delivery of newly synthesized presynaptic proteins, and high rates of synaptic vesicle exo- and endocytosis. Hence, synapses rely on efficient proteostasis mechanisms to preserve their structure and function. To this end, the synaptic compartment has specific chaperones to support its functions...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28577284/overexpression-of-rice-auxilin-like-protein-xb21-induces-necrotic-lesions-up-regulates-endocytosis-related-genes-and-confers-enhanced-resistance-to-xanthomonas-oryzae-pv-oryzae
#17
Chang-Jin Park, Tong Wei, Rita Sharma, Pamela C Ronald
BACKGROUND: The rice immune receptor XA21 confers resistance to the bacterial pathogen, Xanthomonas oryzae pv. oryzae (Xoo). To elucidate the mechanism of XA21-mediated immunity, we previously performed a yeast two-hybrid screening for XA21 interactors and identified XA21 binding protein 21 (XB21). RESULTS: Here, we report that XB21 is an auxilin-like protein predicted to function in clathrin-mediated endocytosis. We demonstrate an XA21/XB21 in vivo interaction using co-immunoprecipitation in rice...
December 2017: Rice
https://www.readbyqxmd.com/read/28566424/neurokinin-1-receptor-signaling-in-endosomes-mediates-sustained-nociception-and-is-a-viable-therapeutic-target-for-prolonged-pain-relief
#18
Dane D Jensen, TinaMarie Lieu, Michelle L Halls, Nicholas A Veldhuis, Wendy L Imlach, Quynh N Mai, Daniel P Poole, Tim Quach, Luigi Aurelio, Joshua Conner, Carmen Klein Herenbrink, Nicholas Barlow, Jamie S Simpson, Martin J Scanlon, Bimbil Graham, Adam McCluskey, Phillip J Robinson, Virginie Escriou, Romina Nassini, Serena Materazzi, Pierangelo Geppetti, Gareth A Hicks, Macdonald J Christie, Christopher J H Porter, Meritxell Canals, Nigel W Bunnett
Typically considered to be cell surface sensors of extracellular signals, heterotrimeric GTP-binding protein (G protein)-coupled receptors (GPCRs) control many pathophysiological processes and are the target of 30% of therapeutic drugs. Activated receptors redistribute to endosomes, but researchers have yet to explore whether endosomal receptors generate signals that control complex processes in vivo and are viable therapeutic targets. We report that the substance P (SP) neurokinin 1 receptor (NK1R) signals from endosomes to induce sustained excitation of spinal neurons and pain transmission and that specific antagonism of the NK1R in endosomes with membrane-anchored drug conjugates provides more effective and sustained pain relief than conventional plasma membrane-targeted antagonists...
May 31, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28565931/histopathology-and-analyses-of-inflammation-intensity-in-the-gills-of-mussels-exposed-to-silver-nanoparticles-role-of-nanoparticle-size-exposure-time-and-uptake-pathways
#19
Younes Bouallegui, Ridha Ben Younes, Houda Bellamine, Ridha Oueslati
Environmentally induced perturbation of health parameters lead to morphological changes associated to the inflammatory response. Hematoxyline and eosin (H&E)-stained gill filaments sections were examined for such changes and inflammation intensity was scored according to a quantitative model in order to evaluate the health status of in vivo exposed (for 3, 6, and 12 h) mussels to silver nanoparticles (Ag-NPs <50 nm and Ag-NPs <100 nm) prior and after the inhibition of two potential uptake pathways (clathrin- and caveolae-mediated endocytosis) with the aid of pharmaceutical inhibitors (amantadine and nystatin)...
June 21, 2017: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/28559042/self-assembled-core-shell-type-lipid-polymer-hybrid-nanoparticles-intracellular-trafficking-and-relevance-for-oral-absorption
#20
Qiuxia Li, Dengning Xia, Jinsong Tao, Aijun Shen, Yuan He, Yong Gan, Chi Wang
Lipid-polymer hybrid nanoparticles (NPs) are advantageous for drug delivery. However, their intracellular trafficking mechanism and relevance for oral drug absorption are poorly understood. In this study, self-assembled core-shell lipid-polymer hybrid NPs made of PLGA and various lipids were developed to study their differing intracellular trafficking in intestinal epithelial cells, and their relevance for oral absorption of a model drug saquinavir (SQV). Our results demonstrated that the endocytosis and exocytosis of hybrid NPs could be changed by varying the kind of lipid...
May 27, 2017: Journal of Pharmaceutical Sciences
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