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Clinical microbiology

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https://www.readbyqxmd.com/read/29036990/-drug-resistance-and-dissemination-of-new-delhi-metallo-%C3%AE-lactamase-1-positive-bacteria-in-a-patient
#1
X F Zhao, M Yuan, X Chen, X F Liu, D S Yu, J Li
Objective: The aim of this work was to report the surveillance and dissemination of NDM-1 positive bacteria in a patient and ward environment. Methods: In 2010, during the therapy for a 51 years old patient, clinical and environmental samples were collected for carbapenem resistant bacterial culture, according to the clinical microbiological examination. Strains identification and antibiotic susceptibility were tested by VITEK Compact 2 system and E-test. The bla(NDM-1) was detected by PCR and analyzed by sequencing...
October 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29036747/prevention-of-multidrug-resistant-infections-from-contaminated-duodenoscopes-position-statement-of-the-european-society-of-gastrointestinal-endoscopy-esge-and-european-society-of-gastroenterology-nurses-and-associates-esgena
#2
Ulrike Beilenhoff, Holger Biering, Reinhard Blum, Jadranka Brljak, Monica Cimbro, Jean-Marc Dumonceau, Cesare Hassan, Michael Jung, Christiane Neumann, Michael Pietsch, Lionel Pineau, Thierry Ponchon, Stanislav Rejchrt, Jean-François Rey, Verona Schmidt, Jayne Tillett, Jeanin van Hooft
Patients should be informed about the benefits and risks of endoscopic retrograde cholangiopancreatography (ERCP)Only specially trained and competent personnel should carry out endoscope reprocessing.Manufacturers of duodenoscopes should provide detailed instructions on how to use and reprocess their equipment.In the case of modifications to their equipment, manufacturers should provide updated instructions for use.Detailed reprocessing protocols based on the manufacturer's instructions for use should clearly lay out the different reprocessing steps necessary for each endoscope model...
October 16, 2017: Endoscopy
https://www.readbyqxmd.com/read/29036724/etiology-and-clinical-characteristics-of-community-acquired-pneumonia-with-airway-malacia-in-children
#3
Wang Yu-Qing, Hao Chuang-Li, Ji Wei, Chen Zheng-Rong, Zhang Xin-Xin, Gu Wen-Jing
Objective: The objective of this article is to study the etiology of community-acquired pneumonia in children with airway malacia. Methods: We retrospectively reviewed the medical records of 428 pneumonia patients. All patients underwent bronchoscopy, and bronchoalveolar lavage samples were processed for microbiological assessment. Results: In a total of 428 cases reviewed, 60 were found to have airway malacia. Pathogens were identified in 44 of the 60 specimens (73...
September 27, 2017: Journal of Tropical Pediatrics
https://www.readbyqxmd.com/read/29034111/molecular-characterization-of-diarrheagenic-escherichia-coli-in-children-less-than-5-years-of-age-with-diarrhea-in-ouagadougou-burkina-faso
#4
Ali Konaté, René Dembélé, Assèta Kagambèga, Issiaka Soulama, Wendpoulomdé A D Kaboré, Emmanuel Sampo, Haoua Cissé, Antoine Sanou, Samuel Serme, Soumanaba Zongo, Cheikna Zongo, Alio Mahamadou Fody, Nathalie K Guessennd, Alfred S Traoré, Amy Gassama-Sow, Nicolas Barro
Diarrheagenic Escherichia coli (DEC) is important bacteria of children's endemic and epidemic diarrhea worldwide. The aim of this study was to determine the prevalence of DEC isolated from stool samples collected from children with acute diarrhea living in Ouagadougou, Burkina Faso. From August 2013 to October 2015, stool samples were collected from 315 children under 5 years of age suffering from diarrhea in the "Centre Médical avec Antenne Chirurgicale (CMA)" Paul VI and the CMA of Schiphra. E. coli were isolated and identified by standard microbiological methods, and the 16-plex PCR method was used to further characterize them...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29033927/editorial-mycoplasma-pneumoniae-clinical-manifestations-microbiology-and-immunology
#5
EDITORIAL
Ran Nir-Paz, Takeshi Saraya, Takashi Shimizu, Annemarie Van Rossum, Cécile Bébéar
No abstract text is available yet for this article.
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29033819/an-unusual-presentation-of-pyoderma-gangrenosum-leading-to-systemic-inflammatory-response-syndrome
#6
Ali Didan, Alan Donnelly, Hock Chua
This is a report of an atypical presentation of pyoderma gangrenosum (PG) in a 26-year-old male who had a negative septic screen. The patient had a life-threatening presentation requiring an intensive care unit (ICU) admission for vasopressor support. It was thought that the likely cause of circulatory collapse was an overwhelming cytokine reaction or systemic inflammatory response syndrome (SIRS) secondary to extensive PG lesions rather than septic shock. The patient presented with multiple large ulcers, the largest being 4 cm in diameter on the central chest...
September 2017: Case Reports in Dermatology
https://www.readbyqxmd.com/read/29033574/surgical-site-infections-following-instrumented-stabilization-of-the-spine
#7
Ulrike Dapunt, Caroline Bürkle, Frank Günther, Wojciech Pepke, Stefan Hemmer, Michael Akbar
BACKGROUND: Implant-associated infections are still a feared complication in the field of orthopedics. Bacteria attach to the implant surface and form so-called biofilm colonies that are often difficult to diagnose and treat. Since the majority of studies focus on prosthetic joint infections (PJIs) of the hip and knee, current treatment options (eg, antibiotic prophylaxis) of implant-associated infections have mostly been adapted according to these results. OBJECTIVE: The aim of this study was to evaluate patients with surgical site infections following instrumented stabilization of the spine with regard to detected bacteria species and the course of the disease...
2017: Therapeutics and Clinical Risk Management
https://www.readbyqxmd.com/read/29033026/economic-impact-of-rapid-diagnostic-methods-in-clinical-microbiology-price-of-the-test-or-overall-clinical-impact
#8
Rafael Cantón, Elia Gómez G de la Pedrosa
The need to reduce the time it takes to establish a microbiological diagnosis and the emergence of new molecular microbiology and proteomic technologies has fuelled the development of rapid and point-of-care techniques, as well as the so-called point-of-care laboratories. These laboratories are responsible for conducting both techniques partially to response to the outsourcing of the conventional hospital laboratories. Their introduction has not always been accompanied with economic studies that address their cost-effectiveness, cost-benefit and cost-utility, but rather tend to be limited to the unit price of the test...
October 13, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29032402/interaction-between-chlorhexidine-and-fluoride-in-a-mouthrinse-solution-a-4-day-and-6-week-randomized-clinical-pilot-study
#9
Oscar Villa, Per Ramberg, Hidekazu Fukui, Claes-Göran Emilson, Georgios Papanikolaou, Lars Heijl, Dowen Birkhed
OBJECTIVE: The aim of the present investigation was to study if there is a reduced clinical effect and fluoride retention of a mouthrinse solution containing both chlorhexidine (CHX) and sodium fluoride (NaF). MATERIAL AND METHODS: Two clinical trials were designed as double-blind, randomized, cross-over studies. In study I, 16 healthy subjects were asked to rinse twice daily for 1 min during 4 days of no tooth brushing with four solutions: (1) 0.12% CHX + 0...
October 14, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29032055/microbiological-characterization-of-klebsiella-pneumoniae-isolates-causing-bloodstream-infection-from-five-tertiary-hospitals-in-beijing-china
#10
Yanning Ma, Chunmei Bao, Jie Liu, Xiuhong Hao, Jingui Cao, Liyan Ye, Jiyong Yang
BACKGROUND: Klebsiella pneumoniae exhibits prevalence in China. Little is known about the microbiological characterization of clinical K. pneumoniae isolates causing bloodstream infections (BSIs). METHODS: BSI-causing K. pneumoniae (BSIKpn) isolates were collected from five tertiary-care hospitals in Beijing, China. Their genetic relatedness was analyzed by pulsed-field gel electrophoresis (PFGE), antimicrobial susceptibility was determined by agar dilution method, and sequence type (ST) was evaluated by multilocus sequence typing (MLST)...
October 11, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29031605/a-waterborne-outbreak-of-multiple-diarrhoeagenic-escherichia-coli-infections-associated-with-drinking-water-at-a-school-camp
#11
Jungsun Park, Jin Seok Kim, Soojin Kim, Eunkyung Shin, Kyung-Hwan Oh, Yonghoon Kim, Cheon Hyeon Kim, Min Ah Hwang, Chan Mun Jin, Kyoungin Na, Jin Lee, Enhi Cho, Byung-Hak Kang, Hyo-Sun Kwak, Won Keun Seong, Junyoung Kim
BACKGROUND: In June 2015, a local public health laboratory was notified that students had developed gastroenteritis symptoms after participating in a camp. An outbreak investigation was conducted to determine the extent and cause of the outbreak. METHOD: We conducted a retrospective cohort study to determine the correlations between the illness and specific exposures at the school camp. All attendees were interviewed with a standard questionnaire that addressed clinical symptoms, food consumption and environmental exposures...
October 11, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29031434/validation-of-an-electronic-tool-for-flagging-surgical-site-infections-based-on-clinical-practice-patterns-for-triaging-surveillance-operational-successes-and-barriers
#12
T Pindyck, K Gupta, J Strymish, K M Itani, M E Carter, Y Suo, M T Bessesen, J Topkoff, A S Steele, A E Barón, A V Gundlapalli, W Branch-Elliman
BACKGROUND: Surveillance is an effective strategy for reducing surgical site infections (SSIs); however, current identification methods are resource-intensive. Therefore, we sought to validate an electronic SSI triaging tool for detection of probable infections and identify operational barriers and challenges. METHODS: A retrospective cohort study was conducted among all Veterans Affairs Surgical Quality Improvement Program (VASQIP)-reviewed surgeries at 2 Veterans Affairs medical centers from October 1, 2011-September 30, 2014...
October 11, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/29031289/histological-examination-in-obtaining-a-diagnosis-in-patients-with-lymphadenopathy-in-lima-peru
#13
Daniela E Kirwan, Cesar Ugarte-Gil, Robert H Gilman, Syed M Hasan Rizvi, Gustavo Cerrillo, Jaime Cok, Eduardo Ticona, José Luis Cabrera, Eduardo D Matos, Carlton A Evans, David A J Moore, Jon S Friedland, The Lymph Node Tuberculosis Lntb Working Group
The differential diagnosis for lymphadenopathy is wide and clinical presentations overlap, making obtaining an accurate diagnosis challenging. We sought to characterize the clinical and radiological characteristics, histological findings, and diagnoses for a cohort of patients with lymphadenopathy of unknown etiology. 121 Peruvian adults with lymphadenopathy underwent lymph node biopsy for microbiological and histopathological evaluation. Mean patient age was 41 years (Interquartile Range 26-52), 56% were males, and 39% were HIV positive...
October 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29030859/outpatient-parenteral-antimicrobial-therapy-in-enterococcus-faecalis-infective-endocarditis
#14
M V Gil-Navarro, L E Lopez-Cortes, R Luque-Marquez, J Galvez-Acebal, A de Alarcon-Gonzalez
WHAT IS KNOWN AND OBJECTIVE: Enterococcus faecalis is the third most common causal agent of infective endocarditis. Currently, the treatment recommended is a combination of ampicillin (2 g/4 h) plus ceftriaxone (2 g/12 h), so patients must remain hospitalized for almost 6 weeks to receive the treatment. They are not generally included in outpatient parenteral antimicrobial therapy programs because 2 different electronic pumps are required to administer these 2 antibiotics. To enable the treatment of patients with E...
October 13, 2017: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/29030168/shiga-toxin-producing-escherichia-coli-incidence-and-clinical-features-in-a-setting-with-complete-screening-of-patients-with-suspected-infective-diarrhoea
#15
Rune Micha Pedersen, Marc Trunjer Kusk Nielsen, Sören Möller, Steen Ethelberg, Marianne Nielsine Skov, Hans Jørn Kolmos, Flemming Scheutz, Hanne Marie Holt, Flemming Schønning Rosenvinge
OBJECTIVES: Shiga toxin-producing Escherichia coli (STEC) causes diarrhoeal disease, bloody diarrhoea and haemolytic uraemic syndrome. The aim of this study was to describe the incidence of STEC and the clinical features of STEC patients from a well-defined Danish population in which all fecal samples of patients with suspected infective gastroenteritis were analysed for STEC. METHODS: In this population-based cohort study, all stool samples referred to two clinical microbiology laboratories, were screened for STEC by culture and/or PCR...
October 10, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29029278/efficacy-and-safety-of-delafloxacin-compared-with-vancomycin-plus-aztreonam-for-acute-bacterial-skin-and-skin-structure-infections-a-phase-3-double-blind-randomized-study
#16
J Pullman, J Gardovskis, B Farley, E Sun, M Quintas, L Lawrence, R Ling, S Cammarata
Background: Delafloxacin is an investigational anionic fluoroquinolone in development for oral or intravenous administration for the treatment of infections caused by Gram-positive (including MRSA), Gram-negative, atypical and anaerobic organisms. Objectives: To establish the non-inferiority of delafloxacin compared with vancomycin plus aztreonam for the treatment of acute bacterial skin and skin structure infections and to compare the safety of the two antimicrobials...
October 5, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29029114/carbapenem-resistant-citrobacter-spp-isolated-in-spain-from-2013-to-2015-produced-a-variety-of-carbapenemases-including-vim-1-oxa-48-kpc-2-ndm-1-and-vim-2
#17
David M Arana, Adriana Ortega, Eva González-Barberá, Noelia Lara, Verónica Bautista, Dolores Gómez-Ruíz, David Sáez, Sara Fernández-Romero, Belén Aracil, María Pérez-Vázquez, José Campos, Jesús Oteo
Objectives: There is little information about carbapenemase-producing (CP) Citrobacter spp. We studied the molecular epidemiology and microbiological features of CP Citrobacter spp. isolates collected in Spain (2013-15). Methods: In total, 119 isolates suspected of being CP by the EUCAST screening cut-off values were analysed. Carbapenemases and ESBLs were characterized using PCR and sequencing. The genetic relationship among Citrobacter freundii isolates was studied by PFGE...
September 27, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29026982/severe-hemoptysis-associated-with-bacterial-pulmonary-infection-clinical-features-significance-of-parenchymal-necrosis-and-outcome
#18
Guillaume Carteaux, Damien Contou, Guillaume Voiriot, Antoine Khalil, Marie-France Carette, Martine Antoine, Antoine Parrot, Muriel Fartoukh
PURPOSE: Severe hemoptysis (SH) associated with non-tuberculosis bacterial lower respiratory tract infection (LRTI) is poorly described, and the efficacy of the usual decision-making process is unknown. This study aimed at describing the clinical, radiological patterns, mechanism, and microbiological spectrum of SH related to bacterial LRTI, and assessing whether the severity of hemoptysis and the results of usual therapeutic strategy are influenced by the presence of parenchymal necrosis...
October 12, 2017: Lung
https://www.readbyqxmd.com/read/29026700/relationship-between-the-remaining-dentin-thickness-and-coronal-pulp-status-of-decayed-primary-molars
#19
Roula Berbari, Hussein Fayyad-Kazan, Mohamad Ezzedine, Mohammad Fayyad-Kazan, Daniel Bandon, Elia Sfeir
AIMS AND OBJECTIVES: The aim of this study was to assess the correlation between the remaining dentin thickness (RDT) in deep decayed primary molars and the inflammatory status and bacterial composition of the corresponding coronal pulp. We hypothesized that RDT could be used as a reference for clinicians in assigning the indication for pulpotomy. MATERIALS AND METHODS: Pulpotomies were conducted on the cameral pulp of 48 primary molars. Microorganisms, such as Lactobacillus sp...
September 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/29026676/spinal-epidural-abscess-report-on-27-cases
#20
Nayil Khursheed, Sultan Dar, Altaf Ramzan, Bashir Fomda, Nisar Humam, Wani Abrar, Sarbjit Singh, Arif Sajad, Masood Mahek, Shoaib Yawar
BACKGROUND: Spinal epidural abscess, although an uncommon disease, often correlates with a high morbidity owing to significant delay in diagnosis. METHODS: In a prospective 5-year study, the clinical and magnetic resonance (MR) findings, treatment protocols, microbiology, and neurological outcomes were analyzed for 27 patients with spinal epidural abscess. RESULTS: Patients were typically middle-aged with underlying diabetes and presented with lumbar abscesses...
2017: Surgical Neurology International
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