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Clinical microbiology

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https://www.readbyqxmd.com/read/29232459/definitive-diagnosis-in-suspected-middle-east-respiratory-syndrome-coronavirus-cases
#1
Elisa Rubio, Miguel J Martínez, Verónica Gonzalo, Josep Barrachina, Núria Torner, Ana I Martínez, Mireia Jané, Anna Vilella, Ana Del Rio, Natalia Rodriguez-Valero, Maria Jesús Pinazo, José Muñoz, Alex Soriano, Antoni Trilla, Jordi Vila, Ma Ángeles Marcos
We evaluated the microbiological diagnosis in 14 patients with epidemiological and clinical suspicion of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) attended in a non-endemic area between June 2015 and January 2017. While no MERS-CoV was detected, other respiratory viruses were identified in 12 cases and Mycoplasma pneumoniae in 1 case.
January 1, 2018: Journal of Travel Medicine
https://www.readbyqxmd.com/read/29229674/efficacy-and-safety-of-linezolid-compared-with-other-treatments-for-skin-and-soft-tissue-infections-a-meta-analysis
#2
Yan Li, Wei Xu
Linezolid with other treatments for skin and soft-tissue infections (SSTIs) has been evaluated in several studies. However, the conclusions remain controversial. By searching PubMed, EMBASE, and Cochrane library databases, we conducted a meta-analysis to evaluate linezolid and other treatments for skin and soft tissue infections. The study was summarized, and the risk ratio (RR) and its 95% confidence interval (CI) were calculated. Eleven related articles were included in the meta-analysis.  Our results revealed that linezolid was associated with a significantly better clinical (RR=1...
December 11, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29229108/infection-control-genetic-assessment-of-drug-resistance-and-drug-susceptibility-testing-in-the-current-management-of-multidrug-extensively-resistant-tuberculosis-m-xdr-tb-in-europe-a-tuberculosis-network-european-trialsgroup-tbnet-study
#3
Graham H Bothamley, Christoph Lange
AIM: Europe has the highest documented caseload and greatest increase in multidrug and extensively drug-resistant tuberculosis (M/XDR-TB) of all World Health Organization (WHO) regions. This survey examines how recommendations for M/XDR-TB management are being implemented. METHODS: TBNET is a pan-European clinical research collaboration for tuberculosis. An email survey of TBNET members collected data in relation to infection control, access to molecular tests and basic microbiology with drug sensitivity testing...
November 2017: Respiratory Medicine
https://www.readbyqxmd.com/read/29228443/postoperative-deep-wound-dehiscence-of-thoracotomy-with-isolation-of-corynebacterium-tuberculostearicum-surgical-site-infection-or-colonization
#4
A Tampakis, E C Tampaki, K Kontzoglou, E Patsouris, G Kouraklis, D Lardinois
OBJECTIVE: Infections with Corynebacterium tuberculostearicum are very rare as in most of the cases its isolation is associated with tissue colonization rather than infection. CASE REPORT: An 80-year old female patient was sent to the consultation hour of thoracic surgery for evaluation of a symptomatic persistent unilateral pleural effusion of her right lung. The differential diagnosis included either the presence of a chronic pleural empyema or the presence of malignancy...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228397/yeast-identification-by-sequencing-biochemical-kits-maldi-tof-ms-and-rep-pcr-dna-fingerprinting
#5
Ying Zhao, Chi-Ching Tsang, Meng Xiao, Jasper F W Chan, Susanna K P Lau, Fanrong Kong, Yingchun Xu, Patrick C Y Woo
No study has comprehensively evaluated the performance of 28S nrDNA and ITS sequencing, commercial biochemical test kits, MALDI-TOF MS platforms, and the emerging rep-PCR DNA fingerprinting technology using a cohort of yeast strains collected from a clinical microbiology laboratory. In this study, using 71 clinically important yeast isolates (excluding Candida albicans) collected from a single centre, we determined the concordance of 28S nrDNA and ITS sequencing and evaluated the performance of two commercial test kits, two MALDI-TOF MS platforms, and rep-PCR DNA fingerprinting...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29227744/molecular-epidemiology-of-klebsiella-pneumoniae-strains-causing-bloodstream-infections-in-adults
#6
Meritxell Cubero, Imma Grau, Fe Tubau, Román Pallarés, M Ángeles Domínguez, Josefina Liñares, Carmen Ardanuy
Molecular epidemiology of Klebsiella pneumoniae bacteremic strains allows for a better understanding of preventive and therapeutic strategies. Clinical and microbiological characteristics of 348 K. pneumoniae bacteremia cases (2007-2009) were retrospectively characterized by multilocus sequence typing and extended-spectrum beta-lactamases (ESBL) production. Overall, 223 (64.08%) cases were nosocomial (NA), 58 (16.67%) healthcare associated, and 67 (19.25%) community acquired. The main infection origins were urinary tract (16...
December 11, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29227741/new-subclass-b1-metallo-%C3%AE-lactamase-gene-from-a-clinical-pathogenic-myroides-odoratus-strain
#7
Su Xu, Yijian Chen, Zhuyingjie Fu, Ying Li, Guixiu Shi, Xiaogang Xu, Yang Liu, Minggui Wang
Myroides odoratus is a low-virulence opportunistic human pathogen. Infections caused by M. odoratus are not common, but reports are increasing in recent years. The biggest challenge for treatment is its resistance to most antibiotics. In 2015, we isolated a pathogenic multidrug-resistant strain of M. odoratus from a urinary tract infection (UTI) patient's urine sample. To report the experience in managing M. odoratus-related UTI and investigate the genetic mechanism of this carbapenem-resistant strain, we conducted a series of microbiological and molecular studies...
December 11, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29226307/macrolides-for-treatment-of-haemophilus-ducreyi-infection-in-sexually-active-adults
#8
REVIEW
Laura Romero, Cesar Huerfano, Carlos F Grillo-Ardila
BACKGROUND: Chancroid is a genital ulcerative disease caused by Haemophilus ducreyi. This microorganism is endemic in Africa, where it can cause up to 10% of genital ulcers. Macrolides may be an effective alternative to treat chancroid and, based on their oral administration and duration of therapy, could be considered as first line therapy. OBJECTIVES: To assess the effectiveness and safety of macrolides for treatment of H ducreyi infection in sexually active adults...
December 11, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29226172/enteric-microbiome-markers-as-early-predictors-of-clinical-outcome-in-allogeneic-hematopoietic-stem-cell-transplant-results-of-a-prospective-study-in-adult-patients
#9
Nicasio Mancini, Raffaella Greco, Renée Pasciuta, Maria Chiara Barbanti, Giacomo Pini, Olivia Beatrice Morrow, Mara Morelli, Luca Vago, Nicola Clementi, Fabio Giglio, Maria Teresa Lupo Stanghellini, Alessandra Forcina, Laura Infurnari, Sarah Marktel, Andrea Assanelli, Matteo Carrabba, Massimo Bernardi, Consuelo Corti, Roberto Burioni, Jacopo Peccatori, Maria Pia Sormani, Giuseppe Banfi, Fabio Ciceri, Massimo Clementi
Background: Infections and graft-vs-host disease (GvHD) still represent major, not easily predictable complications in allogeneic hematopoietic stem cell transplant (allo-HSCT). Both conditions have been correlated to altered enteric microbiome profiles during the peritransplant period. The main objective of this study was to identify possible early microbiome-based markers useful in pretransplant risk stratification. Methods: Stool samples were collected from 96 consecutive patients at the beginning of the pretransplant conditioning regimen (T0) and at 10 (T1) and 30 (T2) days following transplant...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/29225755/evaluation-of-anti-bacterial-effects-of-nickel-nanoparticles-on-biofilm-production-by-staphylococcus-epidermidis
#10
Morteza Vahedi, Nima Hosseini-Jazani, Saber Yousefi, Maryam Ghahremani
Background and Objectives: Staphylococcus epidermidis produces biofilm by extracellular polysaccharides, causing bacterial adherence to different surfaces. Anti-microbial effects of nickel nanoparticles on some bacterial strains such as S. aureus and Escherichia coli have been determined in limited studies. The aim of the present study is to examine the inhibitory effect of nickel nanoparticles on biofilm formation using clinical isolates of S. epidermidis and its hemolytic effect on human red blood cells...
June 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29223965/advancing-prevention-of-sexually-transmitted-infections-through-point-of-care-testing-target-product-profiles-and-landscape-analysis
#11
Igor Toskin, Maurine Murtagh, Rosanna W Peeling, Karel Blondeel, Joanna Cordero, James Kiarie
OBJECTIVES: Advancing the field of point-of-care testing (POCT) for STIs can rapidly and substantially improve STI control and prevention by providing targeted, essential STI services (case detection and screening). POCT enables definitive diagnosis and appropriate treatment in a single visit and home and community-based testing. METHODS: Since 2014, the WHO Department of Reproductive Health and Research, in collaboration with technical partners, has completed four landscape analyses of promising diagnostics for use at or near the point of patient care to detect syphilis, Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis and the human papillomavirus...
December 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29223471/-clinical-epidemiological-study-of-bordetella-pertussis-infection-in-the-gran-canaria-island-in-the-period-2008-2016
#12
Laura Iglesias, Antonio Casabella Pernas, Melisa Hernández Febles, Elena Colino Gil, Alicia Eisman Maraver, María José Pena López
OBJECTIVE: Describe the epidemiological and clinical pattern of Bordetella pertussis infection (whooping cough) among hospitalised infants less than one year-old in a paediatric hospital in Gran Canaria. PATIENTS AND METHODS: A retrospective review of the patient hospital records was performed, and recording only those with a microbiological diagnosis of pertussis infection detected using polymerase chain reaction, from January 2008 to December 2016. RESULTS: A total of 110 patients were identified, of which 105 (95...
December 6, 2017: Anales de Pediatría: Publicación Oficial de la Asociación Española de Pediatría (A.E.P.)
https://www.readbyqxmd.com/read/29223137/sysmex-uf-1000i-flow-cytometer-to-screen-urinary-tract-infections-the-uriscam-multicentre-study
#13
O Herráez, M A Asencio, R Carranza, M M Jarabo, M Huertas, O Redondo, A Arias, S Jiménez-Álvarez, S Solís, P Zamarrón, M S Illescas, M A Galán
The new Sysmex UF-1000i analyzer - which incorporates bacteria morphology distinction - allows to automatically screen samples to be cultured at microbiology laboratories. We have evaluated the feasibility and accuracy of Sysmex UF-1000i to screen urine tract infections (UTIs). A total amount of 2,468 urine samples from six Spanish hospitals were analysed. Demographic and clinical data such as age, gender, source and sample type, preserving conditions, cytometer parameters (bacteria, leucocytes, and bacteria morphology) as well as urine culture results (gold standard) were recorded...
December 9, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29221301/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-a-powerful-tool-for-identification-of-corynebacterium-species
#14
Rong Bao, Xiaodong Gao, Bijie Hu, Zhaoyan Zhou
Background: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful tool that has initiated a revolution in the clinical microbiology laboratory for identification of nosocomial pathogens. The efficacy of MALDI-TOF MS produced by bioMerieux, Marcy l'Etoile, France (VITEK MS) for detecting Corynebacterium remains unknown. Methods: Corynebacterium isolates were isolated from clinical specimen in a tertial teaching hospital from 2012 to 2013...
September 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29218468/prediction-of-unfavorable-outcomes-in-cryptococcal-meningitis-results-of-the-multicenter-infectious-diseases-international-research-initiative-id-iri-cryptococcal-meningitis-study
#15
I N Hakyemez, H Erdem, G Beraud, M Lurdes, A Silva-Pinto, C Alexandru, B Bishop, F Mangani, X Argemi, M Poinot, R Hasbun, M Akcaer, S Alp, T Demirdal, K Angamuthu, F Amer, E Ragab, G A Shehata, D Ozturk-Engin, N Ozgunes, L Larsen, S Zimmerli, O R Sipahi, E Tukenmez Tigen, G Celebi, N Oztoprak, A C Yardimci, Y Cag
Cryptococcal meningitis (CM) is mostly seen in immunocompromised patients, particularly human immunodeficiency virus (HIV)-positive patients, but CM may also occur in apparently immunocompetent individuals. Outcome analyses have been performed in such patients but, due to the high prevalence of HIV infection worldwide, CM patients today may be admitted to hospitals with unknown HIV status, particularly in underdeveloped countries. The objective of this multicenter study was to analyze all types of CM cases in an aggregate cohort to disclose unfavorable outcomes...
December 8, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29217418/molecular-characterization-of-virulence-and-antimicrobial-resistance-profile-of-shigella-species-isolated-from-children-with-moderate-to-severe-diarrhea-in-northeastern-brazil
#16
Pedro Henrique Quintela Soares Medeiros, Aldo Ângelo Moreira Lima, Marjorie Moreira Guedes, Alexandre Havt, Mariana Duarte Bona, Luís Carlos Rey, Alberto Melo Soares, Richard Littleton Guerrant, Bernhard H Weigl, Ila Fernanda Nunes Lima
Molecular characterization of virulence and antimicrobial resistance profiles were determined for Shigella species isolated from children with diarrhea in Fortaleza, Brazil. Fecal specimens were collected along with socioeconomic and clinical data from children with moderate to severe diarrhea requiring emergency care. Shigella spp. were isolated by standard microbiological techniques, and we developed 4 multiplex polymerase chain reaction assays to detect 16 virulence-related genes (VRGs). Antimicrobial susceptibility tests were performed using disk diffusion assays...
November 10, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29217372/acute-peritoneal-dialysis-in-neonatal-intensive-care-unit-an-8-year-experience-of-a-referral-hospital
#17
Aslihan Kara, Metin Kaya Gurgoze, Mustafa Aydin, Erdal Taskin, Unal Bakal, Aysen Orman
BACKGROUND: The aim of present study was to evaluate the indications, complications and outcomes of acute peritoneal dialysis (APD) in neonates at a referral university hospital during the previous 8 years. METHODS: This retrospective analysis included a total of 52 newborn infants who underwent APD in a neonatal intensive care unit between January 2008 and March 2016. Demographic, clinical, laboratory and microbiological data were extracted from patients' medical files...
November 16, 2017: Pediatrics and Neonatology
https://www.readbyqxmd.com/read/29217093/risk-factors-for-the-appearance-of-secondary-foci-of-infection-in-children-with-community-acquired-staphylococcus-aureus-bacteraemia-cohort-study-2010-2016
#18
M Guadalupe Perez, Soledad Martiren, Florencia Escarra, Vanesa Reijtman, Alejandra Mastroianni, Ana Varela-Baino, Carola Cedillo, M Eva Garcia, Moira Taicz, Rosa Bologna
INTRODUCTION: Community-acquired Staphylococcus aureus (SA) bacteraemia is a common cause of hospitalisation in children. The occurrence of secondary foci (SF) of SA infection is associated with higher morbidity and mortality. OBJECTIVES: To identify risk factors for SF of infection in children with community-acquired SA bacteraemia. MATERIAL AND METHODS: Prospective cohort. All children aged from 30 days to 16 years admitted to a paediatric referral hospital between January 2010 and December 2016 for community-acquired infections, with SA isolated in blood cultures, were included...
December 4, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29216923/supervised-learning-for-infection-risk-inference-using-pathology-data
#19
Bernard Hernandez, Pau Herrero, Timothy Miles Rawson, Luke S P Moore, Benjamin Evans, Christofer Toumazou, Alison H Holmes, Pantelis Georgiou
BACKGROUND: Antimicrobial Resistance is threatening our ability to treat common infectious diseases and overuse of antimicrobials to treat human infections in hospitals is accelerating this process. Clinical Decision Support Systems (CDSSs) have been proven to enhance quality of care by promoting change in prescription practices through antimicrobial selection advice. However, bypassing an initial assessment to determine the existence of an underlying disease that justifies the need of antimicrobial therapy might lead to indiscriminate and often unnecessary prescriptions...
December 8, 2017: BMC Medical Informatics and Decision Making
https://www.readbyqxmd.com/read/29216183/presentation-of-life-threatening-invasive-nontyphoidal-salmonella-disease-in-malawian-children-a-prospective-observational-study
#20
Calman A MacLennan, Chisomo L Msefula, Esther N Gondwe, James J Gilchrist, Paul Pensulo, Wilson L Mandala, Grace Mwimaniwa, Meraby Banda, Julia Kenny, Lorna K Wilson, Amos Phiri, Jenny M MacLennan, Elizabeth M Molyneux, Malcolm E Molyneux, Stephen M Graham
Nontyphoidal Salmonellae commonly cause invasive disease in African children that is often fatal. The clinical diagnosis of these infections is hampered by the absence of a clear clinical syndrome. Drug resistance means that empirical antibiotic therapy is often ineffective and currently no vaccine is available. The study objective was to identify risk factors for mortality among children presenting to hospital with invasive Salmonella disease in Africa. We conducted a prospective study enrolling consecutive children with microbiologically-confirmed invasive Salmonella disease admitted to Queen Elizabeth Central Hospital, Blantyre, in 2006...
December 7, 2017: PLoS Neglected Tropical Diseases
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