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https://www.readbyqxmd.com/read/28288847/increased-frequency-and-vasculogenic-potential-of-endothelial-colony-forming-cells-in-patients-with-kaposi-s-sarcoma
#1
Francesca Calcaterra, Lucia Brambilla, Elena Colombo, Athanasia Tourlaki, Stefano Veraldi, Claudia Carenza, Domenico Mavilio, Silvia Della Bella
Kaposi's sarcoma (KS) is characterized by hyperproliferation of spindle cells that have an endothelial origin and assume their characteristic features upon infection with human herpesvirus-8 (HHV8), the causative agent for KS. The multifocal nature of KS suggests that spindle cells derive from circulating HHV8-infected precursors that yet lack identification. We investigated whether ECFCs obtained from KS patients may be putative precursors of spindle cells, by assessing whether their in vitro behavior may evoke the in vivo behavior of KS spindle cells...
March 10, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28276476/mir-193a-3p-interaction-with-hmgb1-downregulates-human-endothelial-cell-proliferation-and-migration
#2
Cheen P Khoo, Maria G Roubelakis, Jack B Schrader, Grigorios Tsaknakis, Rebecca Konietzny, Benedikt Kessler, Adrian L Harris, Suzanne M Watt
Circulating endothelial colony forming cells (ECFCs) contribute to vascular repair where they are a target for therapy. Since ECFC proliferative potential is increased in cord versus peripheral blood and to define regulatory factors controlling this proliferation, we compared the miRNA profiles of cord blood and peripheral blood ECFC-derived cells. Of the top 25 differentially regulated miRNAs selected, 22 were more highly expressed in peripheral blood ECFC-derived cells. After validating candidate miRNAs by q-RT-PCR, we selected miR-193a-3p for further investigation...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28263839/osteoprotegerin-regulates-cancer-cell-migration-through-sdf-1-cxcr4-axis-and-promotes-tumour-development-by-increasing-neovascularization
#3
Zahia Benslimane-Ahmim, Jessica Pereira, Anna Lokajczyk, Blandine Dizier, Isabelle Galy-Fauroux, Anne-Marie Fischer, Dominique Heymann, Catherine Boisson-Vidal
We previously reported that OPG is involved in ischemic tissue neovascularization through the secretion of SDF-1 by pretreated-OPG endothelial colony-forming cells (ECFCs). As the vascularization is one of the key factor influencing the tumour growth and cancer cell dissemination, we investigated whether OPG was able to modulate the invasion of human MNNG-HOS osteosarcoma and DU145 prostate cancer cell lines in vitro and in vivo. Cell motility was analysed in vitro by using Boyden chambers. Human GFP-labelled MMNG-HOS cells were inoculated in immunodeficient mice and the tumour nodules formed were then injected with OPG and/or FGF-2, AMD3100 or 0...
March 3, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28258798/breast-and-renal-cancer-derived-endothelial-colony-forming-cells-share-a-common-gene-signature
#4
Francesco Moccia, Vittoria Fotia, Richard Tancredi, Matteo Giovanni Della Porta, Vittorio Rosti, Elisa Bonetti, Valentina Poletto, Sergio Marchini, Luca Beltrame, Giulia Gallizzi, Gian Antonio Da Prada, Paolo Pedrazzoli, Alberto Riccardi, Camillo Porta, Alberto Zambelli, Maurizio D'Incalci
BACKGROUND: Neovascularisation supports the metastatic switch in many aggressive solid cancers. Tumour neovessels are mostly lined by endothelial cells sprouting from nearby capillaries, but they could also be contributed by circulating endothelial progenitor cells (EPCs). However, scant information is available about tumour-derived EPCs. METHODS: We carried out the first thorough, unbiased comparison of phenotype, function and genotype of normal versus tumour-derived endothelial colony forming cells (ECFCs), a truly endothelial EPC subtype...
February 28, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/28228404/endothelial-colony-forming-cells-ameliorate-endothelial-dysfunction-via-secreted-factors-following-ischemia-reperfusion-injury
#5
Jason A Collett, Purvi Mehrotra, Allison Crone, W Christopher Shelley, Mervin C Yoder, David P Basile
Damage to endothelial cells contributes to acute kidney injury (AKI) by leading to impaired perfusion. Endothelial colony-forming cells (ECFCs) are endothelial precursor cells with high proliferative capacity, pro-angiogenic activity, and in vivo vessel forming potential. We hypothesized that ECFCs may ameliorate the degree of AKI and/or promote repair of the renal vasculature following ischemia/reperfusion (I/R). Rat pulmonary microvascular ECs (PMVEC) with high proliferative potential were compared with pulmonary artery ECs (PAEC) with low proliferative potential in rats subjected to renal I/R...
February 22, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28166726/endothelial-colony-forming-cells-ecfcs-in-murine-aki-implications-for-future-cell-based-therapies
#6
D Patschan, K Schwarze, B Tampe, M Zeisberg, S Patschan, G A Müller
BACKGROUND: In recent years, early Endothelial Progenitor Cells (eEPCs) have been proven as effective tool in murine ischemic AKI and in diabetic nephropathy. The mechanisms of eEPC-mediated vasoprotection have been elucidated in detail. Besides producing a diverse range of humoral factors, the cells also act by secreting vasomodulatory microvesicles. Only few data in contrast have been published about the role of so-called Endothelial Colony Forming Cells (ECFCs - late EPCs) in ischemic AKI...
February 6, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28138561/cd44-expression-in-endothelial-colony-forming-cells-regulates-neurovascular-trophic-effect
#7
Susumu Sakimoto, Valentina Marchetti, Edith Aguilar, Kelsey Lee, Yoshihiko Usui, Salome Murinello, Felicitas Bucher, Jennifer K Trombley, Regis Fallon, Ravenska Wagey, Carrie Peters, Elizabeth L Scheppke, Peter D Westenskow, Martin Friedlander
Vascular abnormalities are a common component of eye diseases that often lead to vision loss. Vaso-obliteration is associated with inherited retinal degenerations, since photoreceptor atrophy lowers local metabolic demands and vascular support to those regions is no longer required. Given the degree of neurovascular crosstalk in the retina, it may be possible to use one cell type to rescue another cell type in the face of severe stress, such as hypoxia or genetically encoded cell-specific degenerations. Here, we show that intravitreally injected human endothelial colony-forming cells (ECFCs) that can be isolated and differentiated from cord blood in xeno-free media collect in the vitreous cavity and rescue vaso-obliteration and neurodegeneration in animal models of retinal disease...
January 26, 2017: JCI Insight
https://www.readbyqxmd.com/read/28122380/dysregulation-of-vascular-endothelial-growth-factor-receptor-2-by-multiple-mirnas-in-endothelial-colony-forming-cells-of-coronary-artery-disease
#8
Shu-Han Su, Chun-Hsien Wu, Ya-Lin Chiu, Shing-Jyh Chang, Hung-Hao Lo, Ko-Hsun Liao, Cheng-Fong Tsai, Tsung-Neng Tsai, Chi-Hung Lin, Shu-Meng Cheng, Cheng-Chung Cheng, Hsei-Wei Wang
BACKGROUND/AIMS: Endothelial colony-forming cells (ECFCs) have the potential to be used in regenerative medicine. Dysfunction of ECFCs is correlated with the onset of cardiovascular disorders, especially coronary artery disease (CAD). Binding of vascular endothelial growth factor A (VEGFA) to vascular endothelial growth factor receptor-2 (VEGFR2) triggers cell motility and angiogenesis of ECFCs, which are crucial to vascular repair. METHODS: To identify the miRNA-VEGFR2-dependent regulation of ECFC functions, ECFCs isolated from peripheral blood of disease-free and CAD individuals were subjected to small RNA sequencing for identification of anti-VEGFR2 miRNAs...
January 26, 2017: Journal of Vascular Research
https://www.readbyqxmd.com/read/28100488/kinetic-analyses-of-vasculogenesis-inform-mechanistic-studies
#9
Kaela M Varberg, Seth Winfree, Chenghao Chu, Wanzhu Tu, Emily K Blue, Cassandra R Gohn, Kenneth W Dunn, Laura S Haneline
Vasculogenesis is a complex process by which endothelial stem and progenitor cells undergo de novo vessel formation. Quantitative assessment of vasculogenesis is a central readout of endothelial progenitor cell (EPC) functionality. However, current assays lack kinetic measurements. To address this issue, new approaches were developed to quantitatively assess in vitro endothelial colony forming cell (ECFC) network formation in real time. Eight parameters of network structure were quantified using novel Kinetic Analysis of Vasculogenesis (KAV) software...
January 18, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28100035/stromal-cells-act-as-guardians-for-endothelial-progenitors-by-reducing-their-immunogenicity-after-co-transplantation
#10
Naima Souidi, Meaghan Stolk, Juliane Rudeck, Dirk Strunk, Katharina Schallmoser, Hans-Dieter Volk, Martina Seifert
Regeneration of injured tissues requires effective therapeutic strategies supporting vasculogenesis. The lack of instantly available autologous cell sources and immunogenicity of allogeneic endothelial (progenitor) cells limits clinical progress. Based on the immunosuppressive potency of mesenchymal stem/progenitor cells (MSCs), we investigated whether crosstalk between endothelial colony-forming progenitor cells (ECFCs) and MSCs during vasculogenesis could lower allogeneic T cell responses against ECFCs allowing long-term engraftment in vivo...
January 18, 2017: Stem Cells
https://www.readbyqxmd.com/read/28072843/chemokine-ligand-5-ccl5-derived-from-endothelial-colony-forming-cells-ecfcs-mediates-recruitment-of-smooth-muscle-progenitor-cells-spcs-toward-critical-vascular-locations-in-moyamoya-disease
#11
Ji Hoon Phi, Naoko Suzuki, Youn Joo Moon, Ae Kyung Park, Kyu-Chang Wang, Ji Yeoun Lee, Seung-Ah Choi, Sangjoon Chong, Reizo Shirane, Seung-Ki Kim
The etiology and pathogenesis of moyamoya disease (MMD) are still obscure. Previous studies indicated that angiogenic chemokines may play an important role in the pathogenesis of the disease. Recently, it was discovered that peripheral blood-derived endothelial colony-forming cells (ECFCs) and smooth muscle progenitor cells (SPCs) have defective functions in MMD patients. Therefore, the interaction of ECFCs and SPCs, the precursors of two crucial cellular components of vascular walls, with some paracrine molecules is an intriguing subject...
2017: PloS One
https://www.readbyqxmd.com/read/28045376/priming-of-endothelial-colony-forming-cells-in-a-mesenchymal-niche-improves-engraftment-and-vasculogenic-potential-by-initiating-mesenchymal-transition-orchestrated-by-notch-signaling
#12
Abbas Shafiee, Jatin Patel, Ho Yi Wong, Prudence Donovan, Dietmar W Hutmacher, Nicholas M Fisk, Kiarash Khosrotehrani
The prospect of using endothelial progenitors is currently hampered by their low engraftment upon transplantation. We report that mesenchymal stem/stromal cells (MSCs), independent of source and age, improve the engraftment of endothelial colony forming cells (ECFCs). MSC coculture altered ECFC appearance to an elongated mesenchymal morphology with reduced proliferation. ECFC primed via MSC contact had reduced self-renewal potential, but improved capacity to form tube structures in vitro and engraftment in vivo Primed ECFCs displayed major differences in transcriptome compared to ECFCs never exposed to MSCs, affecting genes involved in the cell cycle, up-regulating of genes influencing mesenchymal transition, adhesion, extracellular matrix...
October 24, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27966787/mesenchyme-homeobox-2-enhances-migration-of-endothelial-colony-forming-cells-exposed-to-intrauterine-diabetes-mellitus
#13
Cassandra R Gohn, Emily K Blue, BreAnn M Sheehan, Kaela M Varberg, Laura S Haneline
Diabetes mellitus (DM) during pregnancy has long-lasting implications for the fetus, including cardiovascular morbidity. Previously, we showed that endothelial colony forming cells (ECFCs) from DM human pregnancies have decreased vasculogenic potential. Here, we evaluate whether the molecular mechanism responsible for this phenotype involves the transcription factor, Mesenchyme Homeobox 2 (MEOX2). In human umbilical vein endothelial cells, MEOX2 upregulates cyclin dependent kinase inhibitor expression, resulting in increased senescence and decreased proliferation...
December 14, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27899144/hypoxia-induced-responses-by-endothelial-colony-forming-cells-are-modulated-by-placental-growth-factor
#14
Michelle B Hookham, Imran H A Ali, Christina L O'Neill, Emer Hackett, Melanie H Lambe, Tina Schmidt, Reinhold J Medina, Sara Chamney, Bharathi Rao, Eibhlin McLoone, David Sweet, Alan W Stitt, Derek P Brazil
BACKGROUND: Endothelial colony-forming cells (ECFCs), also termed late outgrowth endothelial cells, are a well-defined circulating endothelial progenitor cell type with an established role in vascular repair. ECFCs have clear potential for cell therapy to treat ischaemic disease, although the precise mechanism(s) underlying their response to hypoxia remains ill-defined. METHODS: In this study, we isolated ECFCs from umbilical cord blood and cultured them on collagen...
November 29, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27879452/umbilical-cord-blood-circulating-progenitor-cells-and-endothelial-colony-forming-cells-are-decreased-in-preeclampsia
#15
Diane L Gumina, Claudine P Black, Vivek Balasubramaniam, Virginia D Winn, Christopher D Baker
Preeclampsia (PE) is a pregnancy-specific disease characterized by the new onset of hypertension and proteinuria. Mothers with PE are known to develop endothelial dysfunction, but its effect on infants has been understudied, as newborns are often asymptomatic. Recent studies indicate that infants born from preeclamptic pregnancies develop endothelial dysfunction including higher blood pressure during childhood and an increased risk of stroke later in life. We hypothesize that PE reduces the number and function of fetal angiogenic progenitor cells and may contribute to this increased risk...
November 21, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27832737/oral-mucosa-harbors-a-high-frequency-of-endothelial-cells-a-novel-postnatal-cell-source-for-angiogenic-regeneration
#16
Jian Zhou, Jason H Rogers, Scott H Lee, DongMing Sun, Hai Yao, Jeremy J Mao, Kimi Y Kong
Endothelial progenitor cells/endothelial cells (EPCs/ECs) have great potential to treat pathological conditions such as cardiac infarction, muscle ischemia, and bone fractures, but isolation of EPC/ECs from existing cell sources is challenging due to their low EC frequency. We have isolated endothelial progenitor (EP)-like cells from rat oral mucosa and characterized their yield, immunophenotype, growth, and in vivo angiogenic potential. The frequency of EP-like cells derived from oral mucosa is thousands of folds higher than EPCs derived from donor-match bone marrow samples...
January 15, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/27709781/activation-of-atp-sensitive-potassium-channels-facilitates-the-function-of-human-endothelial-colony-forming-cells-via-ca-2-akt-enos-pathway
#17
Yan Wu, Meng-Yu He, Jian-Kui Ye, Shu-Ying Ma, Wen Huang, Yong-Yue Wei, Hui Kong, Hong Wang, Xiao-Ning Zeng, Wei-Ping Xie
Accumulating data, including those from our laboratory, have shown that the opening of ATP-sensitive potassium channels (KATP ) plays a protective role in pulmonary vascular diseases (PVD). As maintainers of the endothelial framework, endothelial colony-forming cells (ECFCs) are considered excellent candidates for vascular regeneration in cases of PVD. Although KATP openers (KCOs) have been demonstrated to have beneficial effects on endothelial cells, the impact of KATP on ECFC function remains unclear. Herein, this study investigated whether there is a distribution of KATP in ECFCs and what role KATP play in ECFC modulation...
March 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27668588/isolation-of-endothelial-colony-forming-cells-from-blood-samples-collected-from-the-jugular-and-cephalic-veins-of-healthy-adult-horses
#18
Ashley N Sharpe, Wen J Seeto, Randolph L Winter, Qiao Zhong, Elizabeth A Lipke, Anne A Wooldridge
OBJECTIVE To evaluate optimal isolation of endothelial colony-forming cells (ECFCs) from peripheral blood of horses. SAMPLE Jugular and cephalic venous blood samples from 17 adult horses. PROCEDURES Each blood sample was divided; isolation was performed with whole blood adherence (WBA) and density gradient centrifugation (DGC). Isolated cells were characterized by uptake of 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate-labeled acetylated low-density lipoprotein (DiI-Ac-LDL), vascular tubule formation, and expression of endothelial (CD34, CD105, vascular endothelial growth factor receptor-2, and von Willebrand factor) and hematopoietic (CD14) cell markers by use of indirect immunofluorescence assay (IFA) and flow cytometry...
October 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27650731/transfer-of-microrna-486-5p-from-human-endothelial-colony-forming-cell-derived-exosomes-reduces-ischemic-kidney-injury
#19
Jose L Viñas, Dylan Burger, Joseph Zimpelmann, Randa Haneef, William Knoll, Pearl Campbell, Alex Gutsol, Anthony Carter, David S Allan, Kevin D Burns
Administration of human cord blood endothelial colony-forming cells (ECFCs) or their exosomes protects mice against kidney ischemia/reperfusion injury. Here we studied the microRNA (miRNA) content of ECFC exosomes and the role of miRNA transfer in kidney and endothelial cell protection. ECFC exosomes were enriched in miR-486-5p, which targets the phosphatase and tensin homolog (PTEN) and the Akt pathway. In cultured endothelial cells exposed to hypoxia, incubation with ECFC exosomes increased miR-486-5p, decreased PTEN, and stimulated Akt phosphorylation...
December 2016: Kidney International
https://www.readbyqxmd.com/read/27634591/arachidonic-acid-evoked-ca-2-signals-promote-nitric-oxide-release-and-proliferation-in-human-endothelial-colony-forming-cells
#20
Estella Zuccolo, Silvia Dragoni, Valentina Poletto, Paolo Catarsi, Daniele Guido, Alessandra Rappa, Marta Reforgiato, Francesco Lodola, Dmitry Lim, Vittorio Rosti, Germano Guerra, Francesco Moccia
Arachidonic acid (AA) stimulates endothelial cell (EC) proliferation through an increase in intracellular Ca(2+) concentration ([Ca(2+)]i), that, in turn, promotes nitric oxide (NO) release. AA-evoked Ca(2+) signals are mainly mediated by Transient Receptor Potential Vanilloid 4 (TRPV4) channels. Circulating endothelial colony forming cells (ECFCs) represent the only established precursors of ECs. In the present study, we, therefore, sought to elucidate whether AA promotes human ECFC (hECFC) proliferation through an increase in [Ca(2+)]i and the following activation of the endothelial NO synthase (eNOS)...
December 2016: Vascular Pharmacology
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