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https://www.readbyqxmd.com/read/28534915/quantum-dot-based-sensitive-detection-of-disease-specific-exosome-in-serum
#1
Kseniia Boriachek, Md Nazmul Islam, Vinod Gopalan, Alfred K Lam, Nam-Trung Nguyen, Muhammad J A Shiddiky
Tumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific exosomes using quantum dots as signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by a generic tetraspanin antibody (e.g., CD9 or CD63) followed by the identification of disease-specific exosomes using cancer-related...
May 23, 2017: Analyst
https://www.readbyqxmd.com/read/28533221/cd9-regulates-mhc-ii-trafficking-in-monocyte-derived-dendritic-cells
#2
Vera Rocha-Perugini, Gloria Martínez Del Hoyo, José Maria González-Granado, Marta Ramírez-Huesca, Virginia Zorita, Eric Rubinstein, Claude Boucheix, Francisco Sánchez-Madrid
Antigen presentation by dendritic cells (DCs) stimulates naïve CD4(+) T cells, triggering T cell activation and the adaptive arm of the immune response. Newly synthesized major histocompatibility complex class II molecules (MHC-II) accumulate at MHC-II-enriched endosomal compartments, and are transported to the plasma membrane of DCs after binding to antigenic peptides to enable antigen presentation. In DCs, MHC-II molecules are included in tetraspanin-enriched microdomains (TEMs). However, the role of tetraspanin CD9 in these processes remains largely undefined...
May 22, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28507029/cd63-mediated-antigen-delivery-into-extracellular-vesicles-via-dna-vaccination-results-in-robust-cd8-t-cell-responses
#3
Tomohiro Kanuma, Takuya Yamamoto, Kouji Kobiyama, Eiko Moriishi, Yuji Masuta, Takato Kusakabe, Koji Ozasa, Etsushi Kuroda, Nao Jounai, Ken J Ishii
DNA vaccines are attractive immunogens for priming humoral and cellular immune responses to the encoded Ag. However, their ability to induce Ag-specific CD8(+) T cell responses requires improvement. Among the strategies for improving DNA vaccine immunogenicity are booster vaccinations, alternate vaccine formulations, electroporation, and genetic adjuvants, but few, such as extracellular vesicles (EVs), target natural Ag delivery systems. By focusing on CD63, a tetraspanin protein expressed on various cellular membranes, including EVs, we examined whether a DNA vaccine encoding an Ag fused to CD63 delivered into EVs would improve vaccine immunogenicity...
May 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28490497/protein-composition-of-the-hepatitis-a-virus-quasi-envelope
#4
Kevin L McKnight, Ling Xie, Olga González-López, Efraín E Rivera-Serrano, Xian Chen, Stanley M Lemon
The Picornaviridae are a diverse family of RNA viruses including many pathogens of medical and veterinary importance. Classically considered "nonenveloped," recent studies show that some picornaviruses, notably hepatitis A virus (HAV; genus Hepatovirus) and some members of the Enterovirus genus, are released from cells nonlytically in membranous vesicles. To better understand the biogenesis of quasi-enveloped HAV (eHAV) virions, we conducted a quantitative proteomics analysis of eHAV purified from cell-culture supernatant fluids by isopycnic ultracentrifugation...
May 10, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28489901/characterization-and-proteomic-profile-of-extracellular-vesicles-from-peritoneal-dialysis-efflux
#5
Laura Carreras-Planella, Jordi Soler-Majoral, Cristina Rubio-Esteve, Sara Inés Lozano-Ramos, Marcella Franquesa, Josep Bonet, Maria Isabel Troya-Saborido, Francesc Enric Borràs
Peritoneal Dialysis (PD) is considered the best option for a cost-effective mid-term dialysis in patients with Chronic Renal Failure. However, functional failure of the peritoneal membrane (PM) force many patients to stop PD treatment and start haemodialysis. Currently, PM functionality is monitored by the peritoneal equilibration test, a tedious technique that often show changes when the membrane damage is advanced. As in other pathologies, the identification and characterization of extracellular vesicles (EVs) in the peritoneal dialysis efflux (PDE) may represent a non-invasive alternative to identify biomarkers of membrane failure...
2017: PloS One
https://www.readbyqxmd.com/read/28487417/tetraspanin-microdomains-control-localized-protein-kinase-c-signaling-in-b-cells
#6
Malou Zuidscherwoude, Vera-Marie E Dunlock, Geert van den Bogaart, Sjoerd J van Deventer, Alie van der Schaaf, Jenny van Oostrum, Joachim Goedhart, Joanna In 't Hout, Günter J Hämmerling, Satoshi Tanaka, André Nadler, Carsten Schultz, Mark D Wright, Merel J W Adjobo-Hermans, Annemiek B van Spriel
Activation of B cells by the binding of antigens to the B cell receptor (BCR) requires the protein kinase C (PKC) family member PKCβ. Because PKCs must translocate to the plasma membrane to become activated, we investigated the mechanisms regulating their spatial distribution in mouse and human B cells. Through live-cell imaging, we showed that BCR-stimulated production of the second messenger diacylglycerol (DAG) resulted in the translocation of PKCβ from the cytosol to plasma membrane regions containing the tetraspanin protein CD53...
May 9, 2017: Science Signaling
https://www.readbyqxmd.com/read/28473332/cd151-supports-vcam-1-mediated-lymphocyte-adhesion-to-liver-endothelium-and-is-upregulated-in-chronic-liver-disease-and-hepatocellular-carcinoma
#7
James C R Wadkin, Daniel A Patten, Sivesh Kamarajah, Emma L Shepherd, Vera Novitskaya, Fedor Berditchevski, David H Adams, Chris J Weston, Shishir Shetty
CD151, a member of the tetraspanin family of receptors, is a lateral organizer and modulator of activity of several families of transmembrane proteins. It has been implicated in the development and progression of several cancers but its role in chronic inflammatory disease is less well understood. Here we show that CD151 is up-regulated by distinct microenvironmental signals in a range of chronic inflammatory liver diseases and in primary liver cancer where it supports lymphocyte recruitment. CD151 was highly expressed in endothelial cells of the hepatic sinusoids and neovessels developing in fibrotic septa and tumour margins...
May 4, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28458676/tetraspanins-in-plants
#8
REVIEW
Ronny Reimann, Benedikt Kost, Jan Dettmer
Tetraspanins are small transmembrane proteins that laterally associate with each other and cluster with numerous partner proteins as well as lipids. These interactions result in the formation of a distinct class of membrane domains, the tetraspanin-enriched microdomains (TEMs), which influence numerous cellular processes such as cell adhesion and fusion, intracellular membrane trafficking, signaling, morphogenesis, motility as well as interaction with pathogens and cancer development. The majority of information available about tetraspanins is based on studies using animal models or cell lines, but tetraspanins are also present in fungi and plants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28458469/tm4sf5-mediated-roles-in-the-development-of-fibrotic-phenotypes
#9
REVIEW
Jihye Ryu, Jung Weon Lee
Transmembrane 4 L six family member 5 (TM4SF5) can form tetraspanin-enriched microdomains (TERMs) on the cell's surface. TERMs contain protein-protein complexes comprised of tetraspanins, growth factor receptors, and integrins. These complexes regulate communication between extracellular and intracellular spaces to control diverse cellular functions. TM4SF5 influences the epithelial-mesenchymal transition (EMT), aberrant multilayer cellular growth, drug resistance, enhanced migration and invasion, circulation through the bloodstream, tumor-initiation property, metastasis, and muscle development in zebrafish...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28455726/developmental-expression-of-claudins-in-the-mammary-gland
#10
Heidi K Baumgartner, Michael C Rudolph, Palaniappian Ramanathan, Valerie Burns, Patricia Webb, Benjamin G Bitler, Torsten Stein, Ken Kobayashi, Margaret C Neville
Claudins are a large family of membrane proteins whose classic function is to regulate the permeability of tight junctions in epithelia. They are tetraspanins, with four alpha-helices crossing the membrane, two extracellular loops, a short cytoplasmic N-terminus and a longer and more variable C-terminus. The extracellular ends of the helices are known to undergo side-to-side (cis) interactions that allow the formation of claudin polymers in the plane of the membrane. The extracellular loops also engage in head-to-head (trans) interactions thought to mediate the formation of tight junctions...
April 28, 2017: Journal of Mammary Gland Biology and Neoplasia
https://www.readbyqxmd.com/read/28449222/tspan33-is-expressed-in-transitional-and-memory-b-cells-but-is-not-responsible-for-high-adam10-expression
#11
César Augusto Pérez-Martínez, José Luis Maravillas-Montero, Iván Meza-Herrera, Felipe Vences-Catalán, Albert Zlotnik, Leopoldo Santos-Argumedo
Tetraspanins are a family of transmembrane proteins that form membrane microdomains. They play important roles in migration, adhesion, and other cellular processes. TspanC8, a subfamily of tetraspanins, were found to associate and promote ADAM10 trafficking and cell surface localization. One of its members, Tspan33, is expressed in activated B cells. Using RT-PCR and flow cytometry, we analyzed the pattern of expression of Tspan33 in B cells from healthy donors. We found Tspan33 expression in early and late stages of B cell development...
April 27, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28442431/reducing-isoform-complexity-of-human-tetraspanins-by-optimized-expression-in-dictyostelium-discoideum-enables-high-throughput-functional-read-out
#12
Tineke Scheltz, Julia von Bülow, Eric Beitz
The human tetraspanin family of scaffold proteins comprises 33 isoforms. Being integral membrane proteins, they organize a so-called tetraspanin web via homomeric and heteromeric protein-protein interactions with integrins, immunoglobulins, growth factors, receptor tyrosine kinases, proteases, signaling proteins, and viral capsid proteins. Tetraspanins promote cellular effects, such as adhesion, migration, invasion, signaling, membrane fusion, protein trafficking, cancer progression, and infections. The ubiquitous expression of multiple tetraspanin isoforms and partner proteins hampers specific interaction studies...
April 22, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28428953/tetraspanins-function-as-regulators-of-cellular-signaling
#13
REVIEW
Christina M Termini, Jennifer M Gillette
Tetraspanins are molecular scaffolds that distribute proteins into highly organized microdomains consisting of adhesion, signaling, and adaptor proteins. Many reports have identified interactions between tetraspanins and signaling molecules, finding unique downstream cellular consequences. In this review, we will explore these interactions as well as the specific cellular responses to signal activation, focusing on tetraspanin regulation of adhesion-mediated (integrins/FAK), receptor-mediated (EGFR, TNF-α, c-Met, c-Kit), and intracellular signaling (PKC, PI4K, β-catenin)...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28428248/new-insights-into-the-tetraspanin-tspan5-using-novel-monoclonal-antibodies
#14
Julien Saint-Pol, Martine Billard, Emmanuel Dornier, Etienne Eschenbrenner, Lydia Danglot, Claude Boucheix, Stéphanie Charrin, Eric Rubinstein
Tspan5 is a member of a subgroup of tetraspanins referred to as TspanC8. These tetraspanins directly interact with the metalloprotease ADAM10, regulate its exit from the endoplasmic reticulum and subsequent trafficking, and differentially regulate its ability to cleave various substrates and activate Notch signaling. The study of Tspan5 has been limited by the lack of good antibodies. This study provides new insights into Tspan5 using new monoclonal antibodies (mAbs), including two mAbs recognizing both Tspan5 and the highly similar tetraspanin Tspan17...
April 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28423546/tetraspanin-8-tspan-8-as-a-potential-target-for-radio-immunotherapy-of-colorectal-cancer
#15
Aurelie Maisonial-Besset, Tiffany Witkowski, Isabelle Navarro-Teulon, Odile Berthier-Vergnes, Giovanna Fois, Yingying Zhu, Sophie Besse, Olivia Bawa, Arnaud Briat, Mercedes Quintana, Alexandre Pichard, Mathilde Bonnet, Eric Rubinstein, Jean-Pierre Pouget, Paule Opolon, Lydia Maigne, Elisabeth Miot-Noirault, Jean-Michel Chezal, Claude Boucheix, Françoise Degoul
Tetraspanin 8 (TSPAN8) overexpression is correlated with poor prognosis in human colorectal cancer (CRC). A murine mAb Ts29.2 specific for human TSPAN8 provided significant efficiency for immunotherapy in CRC pre-clinical models. We therefore evaluate the feasability of targeting TSPAN8 in CRC with radiolabeled Ts29.2. Staining of tissue micro-arrays with Ts29.2 revealed that TSPAN8 espression was restricted to a few human healthy tissues. DOTA-Ts29.2 was radiolabeled with 111In or 177Lu with radiochemical purities >95%, specific activity ranging from 300 to 600 MBq/mg, and radioimmunoreactive fractions >80%...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28418857/multi-factorial-modulation-of-colorectal-carcinoma-cells-motility-partial-coordination-by-the-tetraspanin-co-029-tspan8
#16
Yingying Zhu, Naouel Ailane, Monica Sala-Valdés, Farhad Haghighi-Rad, Martine Billard, Viet Nguyen, Raphael Saffroy, Antoinette Lemoine, Eric Rubinstein, Claude Boucheix, Céline Greco
Colorectal carcinoma cells Isreco1 display an ability to migrate controlled by a complex set of signals issued from the membrane. By comparing cells infected by mycoplasmas and mycoplasmas free cells, we have established that basal 2D migration is dependent on a double signal mediated by the collagen receptors integrins alpha1/2 and the Toll-Like receptor TLR2. The signal issued from mycoplasmas can be replaced by a TLR2 ligand and the functional effect is neutralized by silencing of MyD88. Following previous observation that downregulation of E-cadherin/p120 catenin increases cell motility, we now report that EGFR or CD44 inhibition have a similar effect on cell motility that is restricted to tetraspanin Co-029/tspan8 transduced IsrecoI cells (Is1-Co029)...
April 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28408492/cd81-as-a-tumor-target
#17
REVIEW
Felipe Vences-Catalán, Caroline Duault, Chiung-Chi Kuo, Ranjani Rajapaksa, Ronald Levy, Shoshana Levy
CD81 participates in a variety of important cellular processes such as membrane organization, protein trafficking, cellular fusion and cell-cell interactions. In the immune system, CD81 regulates immune synapse, receptor clustering and signaling; it also mediates adaptive and innate immune suppression. CD81 is a gateway in hepatocytes for pathogens such as hepatitis C virus and Plasmodium; it also confers susceptibility to Listeria infection. These diverse biological roles are due to the tendency of CD81 to associate with other tetraspanins and with cell-specific partner proteins, which provide the cells with a signaling platform...
April 15, 2017: Biochemical Society Transactions
https://www.readbyqxmd.com/read/28408489/tetraspanins-in-infections-by-human-cytomegalo-and-papillomaviruses
#18
REVIEW
Laura A Fast, Diana Lieber, Thorsten Lang, Luise Florin
Members of the tetraspanin family have been identified as essential cellular membrane proteins in infectious diseases by nearly all types of pathogens. The present review highlights recently published data on the role of tetraspanin CD151, CD81, and CD63 and their interaction partners in host cell entry by human cytomegalo- and human papillomaviruses. Moreover, we discuss a model for tetraspanin assembly into trafficking platforms at the plasma membrane. These platforms might persist during intracellular viral trafficking...
April 15, 2017: Biochemical Society Transactions
https://www.readbyqxmd.com/read/28408487/tspan2-a-tetraspanin-protein-involved-in-oligodendrogenesis-and-cancer-metastasis
#19
REVIEW
Ibrahim H Yaseen, Peter N Monk, Lynda J Partridge
Tetraspanin 2 (Tspan2) is one of the less well-characterised members of the tetraspanin superfamily, and its precise function in different human tissue types remains to be explored. Initial studies have highlighted its possible association in neuroinflammation and carcinogenesis. In the central nervous system, Tspan2 may contribute to the early stages of the oligodendrocyte differentiation into myelin-forming glia. Furthermore, in human lung cancer, Tspan2 could be involved in the progression of the tumour metastasis by modulating cancer cell motility and invasion functions...
April 15, 2017: Biochemical Society Transactions
https://www.readbyqxmd.com/read/28408484/joint-features-and-complementarities-of-tspan8-and-cd151-revealed-in-knockdown-and-knockout-models
#20
REVIEW
Shijing Yue, Kun Zhao, Ulrike Erb, Sanyukta Rana, Margot Zöller
Tetraspanins are highly conserved 4-transmembrane proteins which form molecular clusters with a large variety of transmembrane and cytosolic proteins. By these associations tetraspanins are engaged in a multitude of biological processes. Furthermore, tetraspanin complexes are located in specialized microdomains, called tetraspanin-enriched microdomains (TEMs). TEMs provide a signaling platform and are poised for invagination and vesicle formation. These vesicles can be released as exosomes (Exo) and are important in cell contact-independent intercellular communication...
April 15, 2017: Biochemical Society Transactions
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