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https://www.readbyqxmd.com/read/27901104/development-and-evaluation-of-near-isogenic-lines-for-brown-planthopper-resistance-in-rice-cv-9311
#1
Cong Xiao, Jie Hu, Yi-Ting Ao, Ming-Xing Cheng, Guan-Jun Gao, Qing-Lu Zhang, Guang-Cun He, Yu-Qing He
Brown planthopper (BPH) is the most destructive pest of rice in Asia. To date 29 BPH resistance genes have been identified, but only a few genes are being used in breeding due to inefficient markers for marker-assisted selection (MAS) and little knowledge of the real effects of the genes. In this study we individually transferred 13 genes or QTLs (Bph14, QBph3, QBph4, Bph17, Bph15, Bph20, Bph24, Bph6, Bph3, Bph9, Bph10, Bph18 and Bph21) into cultivar 9311 by marker assisted backcross breeding (MABB). Through positive and negative selection we narrowed the segments from donors containing Bph14, Bph15, Bph6 and Bph9 to 100-400 kb...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27871205/the-effects-of-two-different-swimming-training-periodization-on-physiological-parameters-at-various-exercise-intensities
#2
Vicente Javier Clemente-Suárez, Athanasios Dalamitros, João Ribeiro, Ana Sousa, Ricardo J Fernandes, J Paulo Vilas-Boas
This study analysed the effects of two different periodization strategies on physiological parameters at various exercise intensities in competitive swimmers. Seventeen athletes of both sexes were divided to two groups, the traditional periodization (TPG, n = 7) and the reverse periodization group (RPG, n = 10). Each group followed a 10-week training period based on the two different periodization strategies. Before and after training, swimming velocity (SV), energy expenditure (EE), energy cost (EC) and percentage of aerobic (%Aer) and anaerobic (%An) energy contribution to the swimming intensities corresponding to the aerobic threshold (AerT), the anaerobic threshold (AnT) and the velocity at maximal oxygen uptake (vVO2max) were measured...
November 21, 2016: European Journal of Sport Science
https://www.readbyqxmd.com/read/27862082/influence-of-salt-tolerant-trichoderma-spp-on-growth-of-maize-zea-mays-under-different-salinity-conditions
#3
Krishna Kumar, K Manigundan, Natarajan Amaresan
In the present study, a total of 70 Trichoderma spp. were isolated from the rhizosphere soils of vegetable and spice crops that were grown in Andaman and Nicobar Islands, India. Initial screening of Trichoderma spp. for salt tolerant properties showed 32 isolates were able to tolerate 10% NaCl. Furthermore, these isolates were screened for their potential plant growth-promoting characteristics such as IAA production, phosphate solubilization, and siderophore production. Among 32 isolates, nine isolates were able to produce IAA, siderophore, and solubilize phosphate...
November 8, 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27818142/a-molecular-titration-system-coordinates-ribosomal-protein-gene-transcription-with-ribosomal-rna-synthesis
#4
Benjamin Albert, Britta Knight, Jason Merwin, Victoria Martin, Diana Ottoz, Yvonne Gloor, Maria Jessica Bruzzone, Adam Rudner, David Shore
Cell growth potential is determined by the rate of ribosome biogenesis, a complex process that requires massive and coordinated transcriptional output. In the yeast Saccharomyces cerevisiae, ribosome biogenesis is highly regulated at the transcriptional level. Although evidence for a system that coordinates ribosomal RNA (rRNA) and ribosomal protein gene (RPG) transcription has been described, the molecular mechanisms remain poorly understood. Here we show that an interaction between the RPG transcriptional activator Ifh1 and the rRNA processing factor Utp22 serves to coordinate RPG transcription with that of rRNA...
November 17, 2016: Molecular Cell
https://www.readbyqxmd.com/read/27778337/marker-assisted-introgression-of-broad-spectrum-blast-resistance-genes-into-the-cultivated-mr219-rice-variety
#5
Gous Miah, Mohd Y Rafii, Mohd R Ismail, Adam B Puteh, Harun A Rahim, Mohammad A Latif
BACKGROUND: The rice cultivar MR219 is famous for its better yield and long and fine grain quality; however, it is susceptible to blast disease. The main objective of this study was to introgress blast resistance genes into MR219 through marker-assisted selection (MAS). The rice cultivar MR219 was used as the recurrent parent, and Pongsu Seribu 1 was used as the donor. RESULTS: Marker-assisted foreground selection was performed using RM6836 and RM8225 to identify plants possessing blast resistance genes...
October 24, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27704482/molecular-breeding-of-rice-restorer-lines-and-hybrids-for-brown-planthopper-bph-resistance-using-the-bph14-and-bph15-genes
#6
Hongbo Wang, Shengtuo Ye, Tongmin Mou
BACKGROUND: The development of hybrid rice is a practical approach for increasing rice production. However, the brown planthopper (BPH), Nilaparvata lugens Stål, causes severe yield loss of rice (Oryza sativa L.) and can threaten food security. Therefore, breeding hybrid rice resistant to BPH is the most effective and economical strategy to maintain high and stable production. Fortunately, numerous BPH resistance genes have been identified, and abundant linkage markers are available for molecular marker-assisted selection (MAS) in breeding programs...
December 2016: Rice
https://www.readbyqxmd.com/read/27555861/development-of-gene-pyramid-lines-of-the-elite-restorer-line-rphr-1005-possessing-durable-bacterial-blight-and-blast-resistance
#7
V Abhilash Kumar, C H Balachiranjeevi, S Bhaskar Naik, R Rambabu, G Rekha, G Harika, S K Hajira, K Pranathi, M Anila, M Kousik, S Vijay Kumar, A Yugander, J Aruna, T Dilip Kumar, K Vijaya Sudhakara Rao, A S Hari Prasad, M S Madhav, G S Laha, S M Balachandran, M S Prasad, B C Viraktamath, V Ravindra Babu, R M Sundaram
RPHR-1005, the stable restorer line of the popular medium slender (MS) grain type rice hybrid, DRRH-3 was improved in this study for resistance against bacterial blight (BB) and blast diseases through marker-assisted backcross breeding (MABB). In this study, four major resistance genes (i.e., Xa21 and Xa33 for BB resistance and Pi2 and Pi54 for blast resistance) have been transferred to RPHR-1005 using RPBio Patho-1 (possessing Xa21 + Pi2), RPBio Patho-2 (possessing Xa21 + Pi54) and FBR1-15EM (possessing Xa33) as the donors...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27530580/metabolic-anthropometric-and-type-2-diabetes-mellitus-related-risk-factors-in-normal-and-pre-diabetic-adults
#8
Mohamed H Al-Thani, Heba S Nasser, Suzan Sayegh, Alexandra Haddad, Eman Sadoun
Type 2 diabetes mellitus (T2DM) is a major global health problem. The present study examines the relationship between the metabolic, anthropometric and Finnish risk score (FINDRISC) among normal and pre-diabetic adults. Subjects (n = 1319, aged above 18 years) from the Qatari population were classified into two groups based on their hemoglobin A1c (HbA1c) measurements (non-diabetic A1c<5.6% and pre-diabetic 5.6% ≤ A1c ≤ 6.4%) were examined for their anthropometric (height, weight and waist circumference), metabolic [fat, fat free mass (FFM), muscle mass (MM), total body water (TBW), bone mass, degree of obesity, basal metabolic rate (BMR), body mass index (BMI), metabolic age, visceral fat rating, systolic blood pressure (SBP), diastolic blood pressure (DBP), total cholesterol (Total-C), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TG), fasting / random plasma glucose (FPG / RPG), HbA1c and vitamin D (VitD)] and FINDRISC...
2016: Global Journal of Health Science
https://www.readbyqxmd.com/read/27437922/association-of-random-plasma-glucose-levels-with-the-risk-for-cardiovascular-disease-among-chinese-adults-without-known-diabetes
#9
Fiona Bragg, Liming Li, Derrick Bennett, Yu Guo, Sarah Lewington, Zheng Bian, Ling Yang, Junshi Chen, Yiping Chen, Rory Collins, Richard Peto, Baoyu Zhu, Jiyuan Yin, Ximin Hu, Liyuan Zhou, Yaxing Pan, Zhengming Chen
Importance: Diabetes is a known risk factor for cardiovascular disease (CVD). Substantial uncertainty remains, however, about the relevance to CVD risk for blood glucose levels below the diabetes threshold. Objective: To examine the association of random plasma glucose (RPG) levels with the risk for major CVD in Chinese adults without known diabetes. Design, Setting, and Participants: This prospective cohort study included 467 508 men and women aged 30 to 79 years with no history of diabetes, ischemic heart disease (IHD), stroke, or transient ischemic attack...
July 20, 2016: JAMA Cardiology
https://www.readbyqxmd.com/read/27407019/introgression-of-null-allele-of-kunitz-trypsin-inhibitor-through-marker-assisted-backcross-breeding-in-soybean-glycine-max-l-merr
#10
Shivakumar Maranna, Khushbu Verma, Akshay Talukdar, Sanjay Kumar Lal, Anil Kumar, Keya Mukherjee
BACKGROUND: Presence of Kunitz trypsin inhibitor (KTI) in soybean seeds necessitates pre-heat treatment of the soy-flour for its inactivation before using it in food and feed products. The heat treatment not only enhances processing costs of the soy-based foods and feeds but also affects seed-protein quality and solubility. Genetic elimination of KTI is an important and effective approach. Therefore, molecular marker-assisted backcross breeding (MABB) approach was adopted for genetic elimination of KTI from two popular soybean genotypes, DS9712 and DS9814...
2016: BMC Genetics
https://www.readbyqxmd.com/read/27346035/integrative-analyses-shed-new-light-on-human-ribosomal-protein-gene-regulation
#11
Xin Li, Yiyu Zheng, Haiyan Hu, Xiaoman Li
Ribosomal protein genes (RPGs) are important house-keeping genes that are well-known for their coordinated expression. Previous studies on RPGs are largely limited to their promoter regions. Recent high-throughput studies provide an unprecedented opportunity to study how human RPGs are transcriptionally modulated and how such transcriptional regulation may contribute to the coordinate gene expression in various tissues and cell types. By analyzing the DNase I hypersensitive sites under 349 experimental conditions, we predicted 217 RPG regulatory regions in the human genome...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27262581/multiple-roles-of-the-general-regulatory-factor-abf1-in-yeast-ribosome-biogenesis
#12
Beatrice Fermi, Maria Cristina Bosio, Giorgio Dieci
In Saccharomyces cerevisiae, the large majority of the genes coding for cytoplasmic ribosomal proteins (RPs) depend on the general regulatory factor Rap1 for their transcription, but a small cohort of them relies on Abf1 regulatory activity. A recent study showed that unlike Rap1, whose association with RP gene promoters is not affected by environmental changes causing RP gene repression/reactivation, Abf1 association with both RP gene and ribosome biogenesis (Ribi) gene promoters dynamically responds to changes in growth conditions...
June 4, 2016: Current Genetics
https://www.readbyqxmd.com/read/27214744/simultaneous-determination-of-metformin-glipizide-repaglinide-and-glimepiride-or-metformin-and-pioglitazone-by-a-validated-lc-method-application-in-the-presence-of-metformin-impurity-1-cyanoguanidine
#13
Asmaa A El-Zaher, Ehab F Elkady, Hanan M Elwy, Mahmoud A Saleh
A rapid, simple, and precise RPLC method was developed for the simultaneous determination of the widely used oral antidiabetic, metformin hydrochloride (MTF), with some commonly coadministered oral antidiabetics from different pharmacological classes-glipizide (GPZ), pioglitazone hydrochloride (PGZ), glimepiride (GLM), and repaglinide (RPG)-in bulk, laboratory-prepared mixtures and pharmaceutical formulations in the presence of metformin-reported impurity [1-cyanoguanidine (CNG)]. Chromatographic separation was achieved using isocratic elution mode with a mobile phase of acetonitrile: 0...
July 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/27198549/surgical-advantages-of-reduced-port-laparoscopic-gastrectomy-in-gastric-cancer
#14
Chikara Kunisaki, Hirochika Makino, Naotaka Yamaguchi, Yusuke Izumisawa, Hiroshi Miyamato, Kei Sato, Tsutomu Hayashi, Nobuhiro Sugano, Yoshihiro Suzuki, Mitsuyoshi Ota, Akira Tsuburaya, Jun Kimura, Ryo Takagawa, Takashi Kosaka, Hidetaka Andrew Ono, Hirotoshi Akiyama, Itaru Endo
BACKGROUND: Although a few studies have reported the use of reduced-port laparoscopic gastrectomy (RPG) in gastric cancer patients, the feasibility of routinely using this technique remains unclear. It is therefore important to evaluate the surgical advantages of this technique in this patient group. METHODS: Between August 2010 and July 2015, 165 patients underwent RPGs at our hospital, performed by a single surgeon. Of these patients, 88 underwent reduced-port laparoscopic distal gastrectomy (RPLDG) and 77 underwent reduced-port laparoscopic total gastrectomy (RPLTG)...
December 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/27187792/nanoemulsion-for-improved-oral-delivery-of-repaglinide
#15
Juber Akhtar, Hefazat Hussain Siddiqui, Sheeba Fareed, Badruddeen, Mohammad Khalid, Mohammed Aqil
Repaglinide (RPG) is a fast-acting prandial glucose regulator. It acts by stimulating insulin release from pancreatic β-cells. Recurrent dosing of RPG before each meal is burdensome remedy. Hence the plan of the present study was to evaluate nanoemulsion as a hopeful carrier for RPG for persistent hypoglycemic effect. The drug was incorporated into oil phase of nanoemulsion to give improved biopharmaceutical properties as compared to the lipid-based systems. Pseudo ternary phase diagrams were prepared by aqueous titration method...
July 2016: Drug Delivery
https://www.readbyqxmd.com/read/27156380/facebook-role-play-addiction-a-comorbidity-with-multiple-compulsive-impulsive-spectrum-disorders
#16
Deeepa Nathan, Lekhansh Shukla, Arun Kandasamy, Vivek Benegal
Background Problematic Internet use (PIU) is an emerging entity with varied contents. Behavioral addictions have high comorbidity of attention deficit hyperactivity disorder and obsessive-compulsive spectrum disorders. Social networking site (SNS) addiction and role playing game (RPG) addiction are traditionally studied as separate entities. We present a case with excessive Internet use, with a particular focus on phenomenology and psychiatric comorbidities. Case presentation Fifteen-year-old girl with childhood onset attention deficit disorder, obsessive-compulsive disorder, adolescent onset trichotillomania, and disturbed family environment presented with excessive Facebook use...
June 2016: Journal of Behavioral Addictions
https://www.readbyqxmd.com/read/27097027/splicing-mediated-autoregulation-modulates-rpl22p-expression-in-saccharomyces-cerevisiae
#17
Jason Gabunilas, Guillaume Chanfreau
In Saccharomyces cerevisiae, splicing is critical for expression of ribosomal protein genes (RPGs), which are among the most highly expressed genes and are tightly regulated according to growth and environmental conditions. However, knowledge of the precise mechanisms by which RPG pre-mRNA splicing is regulated on a gene-by-gene basis is lacking. Here we show that Rpl22p has an extraribosomal role in the inhibition of splicing of the RPL22B pre-mRNA transcript. A stem loop secondary structure within the intron is necessary for pre-mRNA binding by Rpl22p in vivo and splicing inhibition in vivo and in vitro and can rescue splicing inhibition in vitro when added in trans to splicing reactions...
April 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27077009/biases-in-grant-proposal-success-rates-funding-rates-and-award-sizes-affect-the-geographical-distribution-of-funding-for-biomedical-research
#18
Wayne P Wahls
The ability of the United States to most efficiently make breakthroughs on the biology, diagnosis and treatment of human diseases requires that physicians and scientists in each state have equal access to federal research grants and grant dollars. However, despite legislative and administrative efforts to ensure equal access, the majority of funding for biomedical research is concentrated in a minority of states. To gain insight into the causes of such disparity, funding metrics were examined for all NIH research project grants (RPGs) from 2004 to 2013...
2016: PeerJ
https://www.readbyqxmd.com/read/27016735/promoter-architecture-and-transcriptional-regulation-of-abf1-dependent-ribosomal-protein-genes-in-saccharomyces-cerevisiae
#19
Beatrice Fermi, Maria Cristina Bosio, Giorgio Dieci
In Saccharomyces cerevisiae, ribosomal protein gene (RPG) promoters display binding sites for either Rap1 or Abf1 transcription factors. Unlike Rap1-associated promoters, the small cohort of Abf1-dependent RPGs (Abf1-RPGs) has not been extensively investigated. We show that RPL3, RPL4B, RPP1A, RPS22B and RPS28A/B share a common promoter architecture, with an Abf1 site upstream of a conserved element matching the sequence recognized by Fhl1, a transcription factor which together with Ifh1 orchestrates Rap1-associated RPG regulation...
July 27, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27005715/glycemic-status-in-organophosphorus-poisoning
#20
S Panda, R Nanda, M Mangaraj, P K Rathod, P K Mishra
BACKGROUND: Organophosphorus(OP) poisoning, in addition to its cholinergic manifestations shows metabolic derangements leading to hyperglycemia. Apart from inhibiting acetylcholinesterase it also induces oxidative stress to exhibit this manifestation. The present study aims to assess the glycemic status of OP poisoned patients and its association with various factors in OP poisoning like oxidative stress and dose of atropine. METHODS: This is a prospective study which recruited 102 patients above 18 years of age with history of OP poisoning...
September 2015: Journal of Nepal Health Research Council
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