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https://www.readbyqxmd.com/read/29243277/purification-of-angiotensin-converting-enzyme-from-human-plasma-and-investigation-of-the-effect-of-some-active-ingredients-isolated-from-nigella-sativa-l-extract-on-the-enzyme-activity
#1
Zehra Basi, Vedat Turkoglu
In the present study, one-step purification of angiotensin converting enzyme (ACE, peptidyldipeptidase A, EC 3.4.15.1), responsible from regulation of blood pressure, was achieved using affinity chromatography from human plasma. The enzyme was purified 12860-fold with a spesific activtiy of 5080 EU/mg.protein. Optimum temperature and pH were determined for the enzyme as 35-40 °C and pH 7.4-7.5, respectively. The purity of ACE was determined by SDS-PAGE and the enzyme showed two bands at 60 kDa and 70 kDa on the gel...
December 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29238025/analytical-methods-to-evaluate-the-quality-of-edible-fats-and-oils-the-jocs-standard-methods-for-analysis-of-fats-oils-and-related-materials-2013-and-advanced-methods
#2
Yasushi Endo
Edible fats and oils are among the basic components of the human diet, along with carbohydrates and proteins, and they are the source of high energy and essential fatty acids such as linoleic and linolenic acids. Edible fats and oils are used in for pan- and deep-frying, and in salad dressing, mayonnaise and processed foods such as chocolates and cream. The physical and chemical properties of edible fats and oils can affect the quality of oil foods and hence must be evaluated in detail. The physical characteristics of edible fats and oils include color, specific gravity, refractive index, melting point, congeal point, smoke point, flash point, fire point, and viscosity, while the chemical characteristics include acid value, saponification value, iodine value, fatty acid composition, trans isomers, triacylglycerol composition, unsaponifiable matters (sterols, tocopherols) and minor components (phospholipids, chlorophyll pigments, glycidyl fatty acid esters)...
December 14, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/29235868/a-metabolomic-signature-of-endometrial-cancer
#3
Jacopo Troisi, Laura Sarno, Annamaria Landolfi, Giovanni Scala, Pasquale Martinelli, Roberta Venturella, Annalisa Di Cello, Fulvio Zullo, Maurizio Guida
Endometrial cancer (EC) is the most common cancer of the female reproductive tract in developed Countries. At the moment, no effective screening system is available. Here, we evaluate the diagnostic performance of a serum metabolomic signature. Two enrollments were carried out: one constituted of 168 subjects: 88 with EC and 80 healthy women, was used for building the classification models. The second (used to establish the performance of the classification algorithm) was constituted of 120 subjects: 30 with EC, 30 with ovarian cancer, 10 with benign endometrial disease and 50 healthy controls...
December 13, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29232927/lutein-esterification-in-wheat-flour-increases-the-carotenoid-retention-and-is-induced-by-storage-temperatures
#4
Elena Mellado-Ortega, Dámaso Hornero-Méndez
The present study aimed to evaluate the effects of long-term storage on the carotenoid pigments present in whole-grain flours prepared from durum wheat and tritordeum. As expected, higher storage temperatures showed a catabolic effect, which was very marked for free carotenoid pigments. Surprisingly, for both cereal genotypes, the thermal conditions favoured the synthesis of lutein esters, leading to an enhanced stability, slower degradation, and, subsequently, a greater carotenoid retention. The putative involvement of lipase enzymes in lutein esterification in flours is discussed, particularly regarding the preferential esterification of the hydroxyl group with linoleic acid at the 3' in the ε-ring of the lutein molecule...
December 11, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29230410/sera-from-visceral-leishmaniasis-patients-display-oxidative-activity-and-affect-the-tnf-%C3%AE-production-by-macrophages-in-vitro
#5
Neci M Soares, Joelma N de Souza, Tatiana F Leal, Eliana A G Reis, Maria S Miranda, Washington L C Dos Santos, Márcia C A Teixeira
Mammalian protection against leishmanial infection depends on the development of an effective immune response. Zoonotic visceral leishmaniasis (ZVL) patients are usually unable to mount an effective immune response against the parasite and indeed appear to be severely immunosuppressed. This suppression has strong nonspecific and specific components mediated by serum factors and leishmanicidal activity of infected macrophages, respectively. The lipid profile has been shown to be altered in ZVL patients' sera...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29229739/complete-assessment-of-whole-body-n-3-and-n-6-pufa-synthesis-secretion-kinetics-and-dha-turnover-in-a-rodent-model
#6
Adam H Metherel, R J Scott Lacombe, Rapha Eumll Chouinard-Watkins, Kathryn E Hopperton, Richard P Bazinet
Previous assessments of the PUFA biosynthesis pathway have focused on DHA and arachidonic acid synthesis. Here, we determined whole-body synthesis-secretion kinetics for all downstream products of PUFA metabolism, including direct measurements of DHA and n-6 docosapentaenoic acid (DPAn-6, 22:5n-6) turnover, and compared n-6 and n-3 homologue kinetics. We infused labeled α-linolenic acid (ALA, 18:3n-3), linoleic acid (LNA, 18:2n-6), DHA and DPAn-6 as 2H5-ALA, 13C18-LNA, 13C22-DHA or 13C22-DPAn-6. Eight 11-week-old Long Evans rats fed a 10% fat diet were infused with the labeled PUFAs over 3 h, and plasma enrichment of labeled products was measured every 30 min...
December 11, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/29227542/characterization-of-peanut-oil-bodies-integral-proteins-lipids-and-their-associated-phytochemicals
#7
Farah Zaaboul, Husnain Raza, Cao Chen, Yuanfa Liu
A microscopic image of a section of a peanut seed shows that oil bodies (OBs) are a small droplet of oil that is dispersed throughout the whole seed. The protein profile of peanut's OBs recovered using the aqueous extraction method at different pH was found to have 2 oleosin isoforms of 14 and 16 kDa. Moreover, OBs essential amino acids are 1.52 higher than those in the PPI. Oleic acid and linoleic acid are the major fatty acids in both cold press peanut oil and OBs regardless of pH. Tocopherol content went from 270...
December 11, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/29227097/interfacial-antioxidants-a-review-of-natural-and-synthetic-emulsifiers-and-co-emulsifiers-that-can-inhibit-lipid-oxidation
#8
David Julian McClements, Eric A Decker
There has been strong interest in developing effective strategies to inhibit lipid oxidation in emulsified food products due to the need to incorporate oxidatively labile bioactive lipids, such as w-3 fatty acids, conjugated linoleic acids, or carotenoids. Emulsifiers or co-emulsifiers can be utilized to inhibit lipid oxidation in emulsions. Both of these molecular types can adsorb to droplet surfaces and inhibit lipid oxidation, but emulsifiers can also stabilize droplets against aggregation whereas co-emulsifiers cannot...
December 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29224879/plant-oil-supplements-reduce-methane-emissions-and-improve-milk-fatty-acid-composition-in-dairy-cows-fed-grass-silage-based-diets-without-affecting-milk-yield
#9
A R Bayat, I Tapio, J Vilkki, K J Shingfield, H Leskinen
Four lipid supplements varying in chain length or degree of unsaturation were examined for their effects on milk yield and composition, ruminal CH4 emissions, rumen fermentation, nutrient utilization, and microbial ecology in lactating dairy cows. Five Nordic Red cows fitted with rumen cannulas were used in a 5 × 5 Latin square with five 28-d periods. Treatments comprised total mixed rations based on grass silage with a forage-to-concentrate ratio of 60:40 supplemented with no lipid (CO) or 50 g/kg of diet dry matter (DM) of myristic acid (MA), rapeseed oil (RO), safflower oil (SO), or linseed oil (LO)...
December 7, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29224335/structure-activity-relationship-sar-of-phenolics-for-2-amino-1-methyl-6-phenylimidazo-4-5-b-pyridine-phip-formation-in-phenylalanine-creatinine-reaction-mixtures-including-or-not-oxygen-and-lipid-hydroperoxides
#10
Francisco J Hidalgo, José L Navarro, Rosario Zamora
Phenolics can act as either promoters or inhibitors in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) formation. In an attempt to clarify the structure-activity relationship (SAR) of phenolics for this reaction, the formation of PhIP in mixtures of phenylalanine, creatinine, 13-hydroperoxide of linoleic acid (LOOH) or 4-oxo-2-nonenal, and a wide array of phenolics was studied in the presence and in the absence of oxygen. The obtained results suggested that those phenolics having a high carbonyl scavenging ability inhibited the formation of PhIP...
December 9, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29221817/polyunsaturated-fatty-acids-in-plasma-at-8%C3%A2-years-and-subsequent-allergic-disease
#11
Jessica Magnusson, Sandra Ekström, Inger Kull, Niclas Håkansson, Sara Nilsson, Magnus Wickman, Erik Melén, Ulf Risérus, Anna Bergström
BACKGROUND: Polyunsaturated fatty acids (PUFAs) are hypothesized to modulate the risk of allergic disease. However, evidence from previous studies is inconclusive, and limited longitudinal data exist using circulating biomarkers of PUFA intake and metabolism. OBJECTIVE: We aimed to investigate associations between n-3 and n-6 PUFAs at age 8 years and asthma, rhinitis, and aeroallergen sensitization at age 16 years. METHODS: Proportions of n-3 PUFAs (very long-chain n-3 [VLC n-3; sum of eicosapentaenoic acid, docosapentaenoic acid, and docosahexaenoic acid] and α-linolenic acid) and n-6 PUFAs (linoleic acid and arachidonic acid [AA]) in blood samples at age 8 years were measured for 940 children from the prospective Swedish birth cohort BAMSE (Children, Allergy, Milieu, Stockholm, Epidemiology)...
November 29, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29219061/effect-of-conjugated-linoleic-acid-supplementation-on-serum-leptin-concentration-a-systematic-review-and-meta-analysis
#12
Fahimeh Haghighatdoost, Mitra Hariri
BACKGROUND: There are controversies regarding the effect of conjugated linoleic acid (CLA) on serum leptin. OBJECTIVE: To conduct a meta-analysis of randomized controlled trials (RCTs) to assess the effect of CLA on serum leptin concentrations. METHOD: Databases such as Ovid, PubMed/Medline, SCOPUS, Google Scholar, and ISI databases up to January 2017 were searched. The searches included RCTs conducted among human adults, and studies on the effect of conjugated linoleic acid on serum leptin concentrations as outcome variables...
December 7, 2017: Endocrine, Metabolic & Immune Disorders Drug Targets
https://www.readbyqxmd.com/read/29218948/-preliminary-screening-of-biomarkers-for-curcumin-s-antidepressant-effect-based-on-metabonomics-method
#13
Zhi-Jie Ma, Wei Zhang, Jie-Ming Dong, Xiao-Hong Yu, Xiao-Mei Zhao, Shi-Biao Pu
To screen potential biomarkers of curcumin related to treating depression rats by using metabolomics means, so as to explore the antidepressant action mechanism of curcumin. The healthy male SD rats were randomly divided into four groups. Chronic unpredictable mild stress (CUMS) stimulation was conducted for modeling for 2 weeks, and then curcumin (200 mg•kg⁻¹) or venlafaxine (40 mg•kg⁻¹) was given by gavage administration. The blank group and model group rats were given with the same volume of 1% CMCNa normal saline, once per day for two weeks...
September 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29213245/implication-of-free-fatty-acids-in-thrombin-generation-and-fibrinolysis-in-vascular-inflammation-in-zucker-rats-and-evolution-with-aging
#14
Jérémy Lagrange, Mélusine Didelot, Amel Mohamadi, Lucy A Walton, Saartje Bloemen, Bas de Laat, Huguette Louis, Simon N Thornton, Brian Derby, Michael J Sherratt, Bruno Fève, Pascal Challande, Riaz Akhtar, J Kennedy Cruickshank, Patrick Lacolley, Véronique Regnault
Background: The metabolic syndrome (MetS) and aging are associated with modifications in blood coagulation factors, vascular inflammation, and increased risk of thrombosis. Objectives: Our aim was to determine concomitant changes in thrombin generation in the blood compartment and at the surface of vascular smooth muscle cells (VSMCs) and its interplay with adipokines, free fatty acids (FFA), and metalloproteinases (MMPs) in obese Zucker rats that share features of the human MetS. Methods: Obese and age-matched lean Zucker rats were compared at 25 and 80 weeks of age...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29213243/mechanism-of-chinese-medicine-herbs-effects-on-chronic-heart-failure-based-on-metabolic-profiling
#15
Kuo Gao, Huihui Zhao, Jian Gao, Binyu Wen, Caixia Jia, Zhiyong Wang, Feilong Zhang, Jinping Wang, Hua Xie, Juan Wang, Wei Wang, Jianxin Chen
Chronic heart failure (CHF) is a major public health problem in huge population worldwide. The detailed understanding of CHF mechanism is still limited. Zheng (syndrome) is the criterion of diagnosis and therapeutic in Traditional Chinese Medicine (TCM). Syndrome prediction may be a better approach for understanding of CHF mechanism basis and its treatment. The authors studied disturbed metabolic biomarkers to construct a predicting mode to assess the diagnostic value of different syndrome of CHF and explore the Chinese herbal medicine (CHM) efficacy on CHF patients...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29211867/effects-of-corn-dried-distillers-grains-with-solubles-on-performance-egg-quality-yolk-fatty-acid-composition-and-oxidative-status-in-laying-ducks
#16
D Ruan, A M Fouad, Q L Fan, W Chen, W G Xia, S Wang, Y Y Cui, Y Wang, L Yang, C T Zheng
The study investigated the effects of increasing content of corn distillers' dried grains with solubles (DDGS) in the diets of laying ducks on oxidative status, laying performance, egg quality, and egg yolk fatty acid composition. Longyan females (1080) with similar BW at 17 wk of age were randomly assigned to 6 treatment groups, each consisting of 6 replicates of 30 birds. The basal diet (I) was a typical corn-soybean ration while the experimental diets (II to VI) substituted corn DDGS for soybean meal and wheat bran and a small reduction of corn...
November 29, 2017: Poultry Science
https://www.readbyqxmd.com/read/29207484/tunisian-milk-thistle-an-investigation-of-the-chemical-composition-and-the-characterization-of-its-cold-pressed-seed-oils
#17
Wiem Meddeb, Leila Rezig, Manef Abderrabba, Gérard Lizard, Mondher Mejri
In this study, milk thistle seeds growing in different areas in Tunisia were cold pressed and the extracted oils were examined for their chemical and antioxidant properties. The major fatty acids were linoleic acid (C18:2) (57.0%, 60.0%, and 60.3% for the milk thistle seed oils native to Bizerte, Zaghouan and Sousse, respectively) and oleic acid (C18:1) (15.5%, 21.5%, and 22.4% for the milk thistle seed oils originating from Bizerte, Zaghouan and Sousse, respectively). High performance liquid chromatography (HPLC) analysis showed the richness of the milk thistle seed oils (MTSO) in α-tocopherol...
December 2, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29206843/periparturient-lipolysis-and-oxylipid-biosynthesis-in-bovine-adipose-tissues
#18
G Andres Contreras, Clarissa Strieder-Barboza, Jonas de Souza, Jeff Gandy, Vengai Mavangira, Adam L Lock, Lorraine M Sordillo
The periparturient period of dairy cows is characterized by intense lipolysis in adipose tissues (AT), which induces the release of free fatty acids (FFA) into circulation. Among FFA, polyunsaturated fatty acids are susceptible to oxidation and can modulate inflammatory responses during lipolysis within AT. Linoleic and arachidonic acid oxidized products (oxylipids) such as hydroxy-octadecadienoic acids (HODE) and hydroxy-eicosatetraenoic acids (HETE), were recently identified as products of lipolysis that could modulate AT inflammation during lipolysis...
2017: PloS One
https://www.readbyqxmd.com/read/29206462/lipidomics-reveals-dramatic-physiological-kinetic-isotope-effects-during-the-enzymatic-oxygenation-of-polyunsaturated-fatty-acids-i-ex-vivo-i
#19
Aaron R Navratil, Mikhail S Shchepinov, Edward A Dennis
Arachidonic acid (AA, 20:4) is an omega-6 polyunsaturated fatty acid (PUFA) and the main precursor to the class of lipid mediators known as eicosanoids. The enzymes that catalyze the oxygenation of AA begin by abstracting hydrogen from one of three bis-allylic carbons within 1,4- cis,cis-diene units. Substitution of deuterium for hydrogen has been shown to lead to massive kinetic isotope effects (KIE) for soybean lipoxygenase (sLOX) oxygenation of linoleic acid (LA, 18:2). Yet, experimental determination of the KIE during oxygenation of AA and LA by mammalian enzymes including cyclooxygenase (COX) and lipoxygenase (LOX) has revealed far lower values...
December 5, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29206180/composition-and-bioactivity-of-essential-oil-from-citrus-grandis-l-osbeck-mato-peiyu-leaf
#20
Mei-Lin Tsai, Cai-Di Lin, Keh Ai Khoo, Mei-Ying Wang, Tsang-Kuei Kuan, Wei-Chao Lin, Ya-Nan Zhang, Ya-Ying Wang
'Mato Peiyu' pomelo (Citrus grandis (L.) Osbeck 'Mato Peiyu') leaves from pruning are currently an agricultural waste. The aim of this study was to isolate essential oils from these leaves through steam distillation (SD) and solvent-free microwave extraction (SFME) and to evaluate their applicability to skin care by analyzing their antimicrobial, antioxidant (diphenyl-1-picrylhydrazyl scavenging assay, β-carotene/linoleic acid assay, and nitric oxide scavenging assay), anti-inflammatory (5-lipoxygenase inhibition assay), and antityrosinase activities...
December 5, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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