keyword
https://read.qxmd.com/read/38536153/prognostic-models-for-mortality-and-morbidity-in-heart-failure-with-preserved-ejection-fraction
#21
JOURNAL ARTICLE
Kirsty McDowell, Toru Kondo, Atefeh Talebi, Ken Teh, Erasmus Bachus, Rudolf A de Boer, Ross T Campbell, Brian Claggett, Ashkay S Desai, Kieran F Docherty, Adrian F Hernandez, Silvio E Inzucchi, Mikhail N Kosiborod, Carolyn S P Lam, Felipe Martinez, Joanne Simpson, Muthiah Vaduganathan, Pardeep S Jhund, Scott D Solomon, John J V McMurray
IMPORTANCE: Accurate risk prediction of morbidity and mortality in patients with heart failure with preserved ejection fraction (HFpEF) may help clinicians risk stratify and inform care decisions. OBJECTIVE: To develop and validate a novel prediction model for clinical outcomes in patients with HFpEF using routinely collected variables and to compare it with a biomarker-driven approach. DESIGN, SETTING, AND PARTICIPANTS: Data were used from the Dapagliflozin Evaluation to Improve the Lives of Patients With Preserved Ejection Fraction Heart Failure (DELIVER) trial to derive the prediction model, and data from the Angiotensin Receptor Neprilysin Inhibition in Heart Failure With Preserved Ejection Fraction (PARAGON-HF) and the Irbesartan in Heart Failure With Preserved Ejection Fraction Study (I-PRESERVE) trials were used to validate it...
March 27, 2024: JAMA Cardiology
https://read.qxmd.com/read/38536089/cloning-and-functional-mechanism-of-the-dwarf-gene-gba-affecting-stem-elongation-and-cellulose-biosynthesis-in-jute-corchorus-olitorius
#22
JOURNAL ARTICLE
Qin Li, Siyuan Chen, Lu Chen, Lingling Zhuang, Huawei Wei, Shaolian Jiang, Chuanyu Wang, Jianmin Qi, Pingping Fang, Jiantang Xu, Aifen Tao, Liwu Zhang
Plant height (PH) is an important factor affecting bast fiber yield in jute. Here, we report the mechanism of dwarfism in the 'Guangbaai' (gba) of jute. The mutant gba had shorter internode length and cell length compared to the standard cultivar 'TaiZi 4' (TZ4). Exogenous GA3 treatment indicated that gba is a GA-insensitive dwarf mutant. Quantitative trait locus (QTL) analysis of three PH-related traits via a high-density genetic linkage map according to re-seq showed that a total of 25 QTLs were identified, including 13 QTLs for PH, with phenotypic variation explained ranging from 2...
March 27, 2024: Plant Journal
https://read.qxmd.com/read/38536076/-pichia-kurtzmaniana-f-a-sp-nov-with-the-transfer-of-eight-candida-species-to-pichia
#23
JOURNAL ARTICLE
Hai-Yan Zhu, Liang-Chen Guo, Shuang Hu, Yu-Hua Wei, Feng-Li Hui, Xin-Zhan Liu, Feng-Yan Bai
Three yeast strains belonging to the ascomycetous yeast genus Pichia were isolated from two soil samples from Yunnan and Guizhou provinces and a marine water sample from Liaoning province, PR China. Phylogenetic analyses based on the sequences of the D1/D2 domains of the large subunit(LSU) rRNA gene and the internal transcribed spacer (ITS) region indicate that these three strains, together with 12 additional strains isolated from various substrates collected in different regions or countries of the world, represent a novel species of the genus Pichia , for which the name Pichia kurtzmaniana sp...
March 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38536042/genetic-variation-in-severe-cystic-fibrosis-liver-disease-is-associated-with-novel-mechanisms-for-disease-pathogenesis
#24
JOURNAL ARTICLE
Jaclyn R Stonebraker, Rhonda G Pace, Paul J Gallins, Hong Dang, Melis A Aksit, Anna V Faino, William W Gordon, Sonya MacParland, Michael J Bamshad, Ronald L Gibson, Garry R Cutting, Peter R Durie, Fred A Wright, Yi-Hui Zhou, Scott M Blackman, Wanda K O'Neal, Simon C Ling, Michael R Knowles
BACKGROUND AND AIMS: It is not known why severe cystic fibrosis (CF) liver disease (CFLD) with portal hypertension occurs in only ~7% of people with CF (pwCF). We aimed to identify genetic modifiers for severe CFLD to improve understanding of disease mechanisms. APPROACH AND RESULTS: Whole genome sequencing was available in 4,082 pwCF with pancreatic insufficiency (n=516 with severe CFLD; n=3,566 without CFLD). We tested ~15.9 million SNPs for association with severe CFLD versus no-CFLD, using pre-modulator clinical phenotypes including: 1) genetic variant (SERPINA1; Z-allele) previously associated with severe CFLD; 2) candidate SNPs (n=205) associated with non-CF liver diseases; 3) genome-wide association study (GWAS) of common/rare SNPs; 4) transcriptome-wide association (TWAS); and 5) gene-level and pathway analyses...
March 27, 2024: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://read.qxmd.com/read/38536021/quality-measures-in-pre-liver-transplant-care-by-the-practice-metrics-committee-of-the-american-association-for-the-study-of-liver-diseases
#25
JOURNAL ARTICLE
Mayur Brahmania, Alexander Kuo, Elliot B Tapper, Michael Volk, Jennifer M Vittorio, Marwan Ghabril, Timothy Morgan, Fasiha Kanwal, Neehar D Parikh, Paul Martin, Shivang Mehta, Gerald S Winder, Gene Y Im, David Goldberg, Jennifer C Lai, Andres Duarte-Rojo, Angelo H Paredes, Arpan A Patel, Amandeep Sahota, Lisa M McElroy, Charlie Thomas, Anji E Wall, Maricar Malinis, Saima Aslam, Douglas A Simonetto, Nneka N Ufere, Sudha Ramakrishnan, Mary Margaret Flynn, Yasmin Ibrahim, Sumeet K Asrani, Marina Serper
INTRODUCTION: The LT evaluation and waitlisting process is subject to variations in care that can impede quality. The American Association for the Study of Liver Diseases (AASLD) Practice Metrics Committee (PMC) developed quality measures and patient-reported experience measures (PREMs) along the continuum of pre-LT care to reduce care variation and guide patient-centered care. METHODS: Following a systematic literature review, candidate pre-LT measures were grouped into four phases of care: referral, evaluation and waitlisting, waitlist management, and organ acceptance...
March 27, 2024: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://read.qxmd.com/read/38535992/native-mass-spectrometry-dissects-the-structural-dynamics-of-an-allosteric-heterodimer-of-sars-cov-2-nonstructural-proteins
#26
JOURNAL ARTICLE
Stephanie M Thibert, Deseree J Reid, Jesse W Wilson, Rohith Varikoti, Natalia Maltseva, Katherine J Schultz, Agustin Kruel, Gyorgy Babnigg, Andrzej Joachimiak, Neeraj Kumar, Mowei Zhou
Structure-based drug design, which relies on precise understanding of the target protein and its interaction with the drug candidate, is dramatically expedited by advances in computational methods for candidate prediction. Yet, the accuracy needs to be improved with more structural data from high throughput experiments, which are challenging to generate, especially for dynamic and weak associations. Herein, we applied native mass spectrometry (native MS) to rapidly characterize ligand binding of an allosteric heterodimeric complex of SARS-CoV-2 nonstructural proteins (nsp) nsp10 and nsp16 (nsp10/16), a complex essential for virus survival in the host and thus a desirable drug target...
March 27, 2024: Journal of the American Society for Mass Spectrometry
https://read.qxmd.com/read/38535984/italian-society-of-interventional-cardiology-gise-and-italian-society-of-arterial-hypertension-siia-position-paper-on-the-role-of-renal-denervation-in-the-management-of-the-difficult-to-treat-hypertension
#27
JOURNAL ARTICLE
Eugenio Stabile, Maria L Muiesan, Flavio L Ribichini, Giuseppe Sangiorgi, Stefano Taddei, Francesco Versaci, Bruno Villari, Alessandra Bacca, Daniela Benedetto, Vincenzo Fioretti, Eugenio Laurenzano, Massimilano Scapaticci, Francesco Saia, Giuseppe Tarantini, Guido Grassi, Giovanni Esposito
Renal denervation (RDN) is a safe and effective strategy for the treatment of difficult to treat hypertension. The blood pressure (BP)-lowering efficacy of RDN is comparable to those of many single antihypertensive medications and it allows to consider the RDN as a valuable option for the treatment of difficult to treat hypertension together with lifestyle modifications and medical therapy. A multidisciplinary team is of pivotal importance from the selection of the patient candidate for the procedure to the post-procedural management...
March 27, 2024: Minerva cardiology and angiology
https://read.qxmd.com/read/38535952/a-proof-of-concept-for-a-hypolipidemic-brown-trout-model
#28
JOURNAL ARTICLE
Tiago Lourenço, Eduardo Rocha, José Fernando Gonçalves, Maria João Rocha, Tânia Vieira Madureira
The impacts of hypolipidemic pharmaceuticals on fish lipid metabolism remain unexplored. However, data points to similar effects and mechanisms of action between fish and humans. Therefore, fish may be a strong model for screening hypolipidemic drug candidates and water pollution by lipid-modulating agents. This study aimed to test a new hypolipidemic model assay with juvenile brown trout using atorvastatin (ATV)-a hypolipidemic chemical. We selected 17α-ethinylestradiol (EE2), known to cause hyperlipidemia in fish, to ensure model functionality...
March 15, 2024: Toxics
https://read.qxmd.com/read/38535895/a-multi-colour-fluorogenic-tag-and-its-application-in-candida-albicans
#29
JOURNAL ARTICLE
Jonas Devos, Patrick Van Dijck, Wouter Van Genechten
Fluorescent proteins (FPs) have always been a crucial part of molecular research in life sciences, including the research into the human fungal pathogen Candida albicans, but have obvious shortcomings such as their relatively large size and long maturation time. However, the next generation of FPs overcome these issues and rely on the binding of a fluorogen for the protein to become fluorescently active. This generation of FPs includes the improved version of Fluorescence activating and Absorption Shifting Tag (iFAST)...
March 2024: Microbiology
https://read.qxmd.com/read/38535857/molecular-detection-of-candidatus-anaplasma-camelii-in-naturally-infected-dromedary-camels-camelus-dromedarius-in-abu-dhabi-emirate-united-arab-emirates-2019-2023
#30
JOURNAL ARTICLE
Hassan Zackaria Ali Ishag, Shameem Habeeba, El Tigani Ahmed El Tigani-Asil, Mohd Farouk Yuosf, Zulaikha Mohamed Abdel Hameed Al Hammadi, Abraham Nii Okai Commey, Hashel Talal Aboud Amer Bin Hraiz, Asma Abdi Mohamed Shah, Abdelmalik Ibrahim Khalafalla
The recent emergence of anaplasmosis in camels has raised global interest in the pathogenicity and zoonotic potential of the pathogen causing it and the role of camels as reservoir hosts. In the United Arab Emirates (UAE), molecular studies and genetic characterization of camel-associated Anaplasma species are limited. This study aimed to characterize molecularly Anaplasmataceae strains circulating in dromedary camels in the UAE. Two hundred eighty-seven whole-blood samples collected from dromedary camels across regions of the Abu Dhabi Emirate were received between 2019 and 2023 at the Abu Dhabi Agriculture and Food Safety Authority (ADAFSA) veterinary laboratories for routine diagnosis of anaplasmosis...
March 7, 2024: Veterinary Sciences
https://read.qxmd.com/read/38535816/diversity-and-molecular-evolution-of-antimicrobial-peptides-in-caecilian-amphibians
#31
JOURNAL ARTICLE
Mario Benítez-Prián, Héctor Lorente-Martínez, Ainhoa Agorreta, David J Gower, Mark Wilkinson, Kim Roelants, Diego San Mauro
Antimicrobial peptides (AMPs) are key molecules in the innate immune defence of vertebrates with rapid action, broad antimicrobial spectrum, and ability to evade pathogen resistance mechanisms. To date, amphibians are the major group of vertebrates from which most AMPs have been characterised, but most studies have focused on the bioactive skin secretions of anurans (frogs and toads). In this study, we have analysed the complete genomes and/or transcriptomes of eight species of caecilian amphibians (order Gymnophiona) and characterised the diversity, molecular evolution, and antimicrobial potential of the AMP repertoire of this order of amphibians...
March 14, 2024: Toxins
https://read.qxmd.com/read/38535807/inhibition-of-aflatoxin-production-in-aspergillus-flavus-by-a-klebsiella-sp-and-its-metabolite-cyclo-l-ala-gly
#32
JOURNAL ARTICLE
Shohei Sakuda, Masaki Sunaoka, Maho Terada, Ayaka Sakoda, Natsumi Ishijima, Noriko Hakoshima, Kenichi Uchida, Hirofumi Enomoto, Tomohiro Furukawa
During an experiment where we were cultivating aflatoxigenic Aspergillus flavus on peanuts, we accidentally discovered that a bacterium adhering to the peanut strongly inhibited aflatoxin (AF) production by A. flavus . The bacterium, isolated and identified as Klebsiella aerogenes , was found to produce an AF production inhibitor. Cyclo(l-Ala-Gly), isolated from the bacterial culture supernatant, was the main active component. The aflatoxin production-inhibitory activity of cyclo(l-Ala-Gly) has not been reported...
March 8, 2024: Toxins
https://read.qxmd.com/read/38535798/function-and-mechanism-of-antiviral-wasp-venom-peptide-protopolybia-mp-iii-and-its-derivatives-against-hsv-1
#33
JOURNAL ARTICLE
Fang Sun, Xiangdong Ye, Tanran Han, Jingwen Xia, Lili Wu, Wen Zhu, Li Ding, Xudong Luo, Chenhu Qin, Zongyun Chen
Viruses are one of the leading causes of human disease, and many highly pathogenic viruses still have no specific treatment drugs. Therefore, producing new antiviral drugs is an urgent matter. In our study, we first found that the natural wasp venom peptide Protopolybia-MP III had a significant inhibitory effect on herpes simplex virus type 1 (HSV-1) replication in vitro by using quantitative real-time PCR (qPCR), Western blotting, and plaque-forming assays. Immunofluorescence analysis showed Protopolybia-MP III could enter cells, and it inhibited multiple stages of the HSV-1 life cycle, including the attachment, entry/fusion, and post-entry stages...
March 4, 2024: Toxins
https://read.qxmd.com/read/38535797/mycoviruses-increase-the-attractiveness-of-fusarium-graminearum-for-fungivores-and-suppress-production-of-the-mycotoxin-deoxynivalenol
#34
JOURNAL ARTICLE
Simon Schiwek, Matthäus Slonka, Mohammad Alhussein, Dennis Knierim, Paolo Margaria, Hanna Rose, Katja R Richert-Pöggeler, Michael Rostás, Petr Karlovsky
RNA viruses of the genera Ambivirus , Mitovirus , Sclerotimonavirus , and Partitivirus were found in a single isolate of Fusarium graminearum . The genomes of the mitovirus, sclerotimonavirus, and partitivirus were assigned to previously described viruses, whereas the ambivirus genome putatively represents a new species, named Fusarium graminearum ambivirus 1 (FgAV1). To investigate the effect of mycoviruses on the fungal phenotype, the spontaneous loss of mycoviruses during meiosis and the transmission of mycoviruses into a new strain via anastomosis were used to obtain isogenic F...
March 2, 2024: Toxins
https://read.qxmd.com/read/38535704/effect-of-strain-rate-temperature-vacancy-and-microcracks-on-mechanical-properties-of-8-16-4-graphyne
#35
JOURNAL ARTICLE
Qing Peng, Zeyu Huang, Gen Chen, Yuqiang Zhang, Xiaofan Zhang, Xiao-Jia Chen, Zhongwei Hu
The 8-16-4 graphyne, a recently identified two-dimensional carbon allotrope, exhibits distinctive mechanical and electrical properties, making it a candidate material for flexible electronic applications. This study endeavors to enhance our comprehension of the fracture behavior and mechanical properties of 8-16-4 graphyne. The mechanical properties of 8-16-4 graphyne were evaluated through molecular dynamics simulations, examining the impact of boundary conditions, temperature, and strain rate, as well as the coupled interactions between temperature, vacancy defects, and microcracks...
March 21, 2024: Nanomaterials
https://read.qxmd.com/read/38535694/a-conductive-microcavity-created-by-assembly-of-carbon-nanotube-buckypapers-for-developing-electrochemically-wired-enzyme-cascades
#36
JOURNAL ARTICLE
Itthipon Jeerapan, Yannig Nedellec, Serge Cosnier
We describe the creation of a conductive microcavity based on the assembly of two pieces of carbon nanotube buckypaper for the entrapment of two enzymes, horseradish peroxidase (HRP) and glucose oxidase (GOx), as well as a redox mediator: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS). The hollow electrode, employing GOx, HRP, and the mediator, as an electrochemical enzyme cascade model, is utilized for glucose sensing at a potential of 50 mV vs. Ag/AgCl. This bienzyme electrode demonstrates the ability to oxidize glucose by GOx and subsequently convert H2 O2 to water via the electrical wiring of HRP by ABTS...
March 20, 2024: Nanomaterials
https://read.qxmd.com/read/38535625/targeted-and-self-adjuvated-nanoglycovaccine-candidate-for-cancer-immunotherapy
#37
JOURNAL ARTICLE
Rui Freitas, Eduardo Ferreira, Andreia Miranda, Dylan Ferreira, Marta Relvas-Santos, Flávia Castro, Beatriz Santos, Martina Gonçalves, Sofia Quintas, Andreia Peixoto, Carlos Palmeira, André M N Silva, Lúcio Lara Santos, Maria José Oliveira, Bruno Sarmento, José Alexandre Ferreira
Advanced-stage solid primary tumors and metastases often express mucin 16 (MUC16), carrying immature glycans such as the Tn antigen, resulting in specific glycoproteoforms not found in healthy human tissues. This presents a valuable approach for designing targeted therapeutics, including cancer glycovaccines, which could potentially promote antigen recognition and foster the immune response to control disease spread and prevent relapse. In this study, we describe an adjuvant-free poly(lactic- co -glycolic acid) (PLGA)-based nanoglycoantigen delivery approach that outperforms conventional methods by eliminating the need for protein carriers while exhibiting targeted and adjuvant properties...
March 27, 2024: ACS Nano
https://read.qxmd.com/read/38535612/fungemia-by-wickerhamomyces-anomalus-a-narrative-review
#38
REVIEW
Petros Ioannou, Stella Baliou, Diamantis P Kofteridis
Wickerhamomyces anomalus has been previously classified as Hansenula anomala , Pichia anomala , and Candida pelliculosa and was recently reclassified in the genus Wickerhamomyces after phylogenetic analysis of its genetic sequence. An increasing number of reports of human infections by W. anomalus have emerged, suggesting that this microorganism is an emerging pathogen. The present review aimed to provide data on the epidemiology, antifungal resistance, clinical characteristics, treatment, and outcomes of fungemia by W...
March 21, 2024: Pathogens
https://read.qxmd.com/read/38535596/the-influence-of-l-lysine-alpha-oxidase-on-the-biofilm-formation-of-opportunistic-microorganisms-associated-with-inflammatory-diseases-of-the-urinary-tract
#39
JOURNAL ARTICLE
Alexandr Senyagin, Nadezhda Sachivkina, Milana Das, Anna Arsenyuk, Ramziya Mannapova, Alfir Mannapov, Tursumbai Kubatbekov, Dmitriy Svistunov, Olesya Petrukhina, Andrey Zharov, Natallia Zhabo
Urinary tract infections occupy a special niche among diseases of infectious etiology. Many microorganisms associated with urinary tract infections, such as Klebsiella oxytoca , Enterococcus spp., Morganella morganii , Moraxella catarrhalis , Pseudomonas aeruginosa , Proteus mirabilis , Staphylococcus aureus , Staphylococcus spp., and Candida spp., can form biofilms. The aim of this research was to study the effect of the enzyme L-lysine-Alpha-oxidase (LO) produced by the fungus Trichoderma harzianum Rifai on the biofilm formation process of microorganisms associated with urinary tract infections...
March 15, 2024: Pathogens
https://read.qxmd.com/read/38535576/-galleria-mellonella-as-a-model-for-the-study-of-fungal-pathogens-advantages-and-disadvantages
#40
REVIEW
Andrea Giammarino, Nicolò Bellucci, Letizia Angiolella
The study of pathogenicity and virulence of fungal strains, in vivo in the preclinical phase, is carried out through the use of animal models belonging to various classes of mammals (rodents, leproids, etc.). Although animals are functionally more similar to humans, these studies have some limitations in terms of ethics (animal suffering), user-friendliness, cost-effectiveness, timing (physiological response time) and logistics (need for adequately equipped laboratories). A good in vivo model must possess some optimal characteristics to be used, such as rapid growth, small size and short life cycle...
March 7, 2024: Pathogens
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