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https://www.readbyqxmd.com/read/28215034/transcription-factor-afmac1-controls-copper-import-machinery-in-aspergillus-fumigatus
#1
Yoko Kusuya, Daisuke Hagiwara, Kanae Sakai, Takashi Yaguchi, Tohru Gonoi, Hiroki Takahashi
Copper (Cu) is an essential metal for all living organisms, although it is toxic in excess. Filamentous fungus must acquire copper from its environment for growth. Despite its essentiality for growth, the mechanisms that maintain copper homeostasis are not fully understood in filamentous fungus. To gain insights into copper homeostasis, we investigated the roles of a copper transcription factor Afmac1 in the life-threatening fungus Aspergillus fumigatus, a homolog of the yeast MAC1. We observed that the Afmac1 deletion mutant exhibited not only significantly slower growth, but also incomplete conidiation including a short chain of conidia and defective melanin...
February 18, 2017: Current Genetics
https://www.readbyqxmd.com/read/28214445/immobilization-of-aspergillus-oryzae-%C3%AE-galactosidase-in-an-agarose-matrix-functionalized-by-four-different-methods-and-application-to-the-synthesis-of-lactulose
#2
Cecilia Guerrero, Carlos Vera, Nestor Serna, Andrés Illanes
Aspergillus oryzae β-galactosidase was immobilized in monofunctional glyoxyl-agarose and heterofunctional supports (amino-glyoxyl, carboxy-glyoxyl and chelate-glyoxyl agarose), for obtaining highly active and stable catalysts for lactulose synthesis. Specific activities of the amino-glyoxyl agarose, carboxy-glyoxyl agarose and chelate-glyoxyl agarose derivatives were 3676, 430 and 454IU/g biocatalyst with half-life values at 50°C of 247, 100 and 100h respectively. Specific activities of 3490, 2559 and 1060IU/g were obtained for fine, standard and macro agarose respectively...
February 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28213885/epigenetic-modifier-induced-enhancement-of-fumiquinazoline-c-production-in-aspergillus-fumigatus-ga-l7-an-endophytic-fungus-from-grewia-asiatica-l
#3
Ankita Magotra, Manjeet Kumar, Manoj Kushwaha, Praveen Awasthi, Chand Raina, Ajai Prakash Gupta, Bhahwal A Shah, Sumit G Gandhi, Asha Chaubey
Present study relates to the effect of valproic acid, an epigenetic modifier on the metabolic profile of Aspergillus fumigatus (GA-L7), an endophytic fungus isolated from Grewia asiatica L. Seven secondary metabolites were isolated from A. fumigatus (GA-L7) which were identified as: pseurotin A, pseurotin D, pseurotin F2, fumagillin, tryprostatin C, gliotoxin and bis(methylthio)gliotoxin. Addition of valproic acid in the growth medium resulted in the alteration of secondary metabolic profile with an enhanced production of a metabolite, fumiquinazoline C by tenfolds...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28213800/invasive-aspergillus-laryngotracheobronchitis-in-an-adult-with-primary-cns-lymphoma
#4
Mark E Barry, William Walsh Thomas, Buer Song, Natasha Mirza
Invasive aspergillosis can be difficult to diagnose, and early recognition and initiation of therapy is imperative for improving patient outcomes. A case of invasive Aspergillus laryngotracheobronchitis is presented here with a review of the relevant literature. A 58-year-old male undergoing treatment for CNS lymphoma presented with neutropenic sepsis and acute respiratory distress requiring intubation. Following extubation, he reported persistent hoarseness for 1-month duration and he was found to have pseudomembranous plaques and ulcers of the larynx, trachea, and right mainstem bronchus consistent with Aspergillus laryngotracheobronchitis...
February 17, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28213689/serious-fungal-infections-in-egypt
#5
S M Zaki, D W Denning
We aimed to estimate the burden of serious fungal infections in Egypt, currently unknown, based on the size of the populations at risk and available epidemiological data. Data were obtained from the World Health Organization (WHO), the Joint United Nations Programme on HIV/AIDS (UNAIDS), and published reports with clearcut denominators. When no data existed, risk populations were used to estimate frequencies of fungal infections, using previously described methodology. The population of Egypt in 2011 was ∼82,500,000; 31% children, and 8% women >60 years of age...
February 17, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28213139/antimycobacterial-antimicrobial-activity-experimental-ft-ir-ft-raman-nmr-uv-vis-dsc-and-dft-transition-state-chemical-reactivity-nbo-nlo
#6
Poonam Rawat, R N Singh, Alok Ranjan, Sartaj Ahmad, Rajat Saxena
As part of a study of pyrrole hydrazone, we have investigated quantum chemical calculations, molecular geometry, relative energy, vibrational properties and antimycobacterial/antimicrobial activity of pyrrole-2-carboxaldehyde isonicotinyl hydrazone (PCINH), by applying the density functional theory (DFT) and Hartree Fock (HF). Good reproduction of experimental values is obtained and with small percentage error in majority of the cases in comparison to theoretical result (DFT). The experimental FT-IR and Raman wavenumbers were compared with the respective theoretical values obtained from DFT calculations and found to agree well...
February 11, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28213038/hydrolysate-from-a-mixture-of-legume-flours-with-antifungal-activity-as-an-ingredient-for-prolonging-the-shelf-life-of-wheat-bread
#7
Carlo Giuseppe Rizzello, Michela Verni, Stefano Bordignon, Valerio Gramaglia, Marco Gobbetti
Aiming at identifying antifungal compounds from plant matrices to be used as ingredients in the bakery industry, a water/salt-soluble extract (WSE) was produced from a legume enzyme hydrolysate, consisting of a mixture of pea, lentil, and faba bean flours, and assayed towards Penicillium roqueforti DPPMAF1. Agar diffusion assays allowed the selection of the optimal processing conditions for hydrolysis. As shown by hyphal radial growth rate, the inhibition was observed towards several fungi, including Aspergillus parasiticus CBS971...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28213013/time-course-of-germination-initiation-of-mycelium-proliferation-and-probability-of-visible-growth-and-detectable-afb1-production-of-an-isolate-of-aspergillus-flavus-on-pistachio-extract-agar
#8
Laila Aldars-García, Vicente Sanchis, Antonio J Ramos, Sonia Marín
The aim of this work was to assess the temporal relationship among quantified germination, mycelial growth and aflatoxin B1 (AFB1) production from colonies coming from single spores, in order to find the best way to predict as accurately as possible the presence of AFB1 at the early stages of contamination. Germination, mycelial growth, probability of growth and probability of AFB1 production of an isolate of Aspergillus flavus were determined at 25 °C and two water activities (0.85 and 0.87) on 3% Pistachio Extract Agar (PEA)...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28212851/the-high-affinity-phosphodiesterase-pdeh-regulates-development-and-aflatoxin-biosynthesis-in-aspergillus-flavus
#9
Kunlong Yang, Yinghang Liu, Linlin Liang, Zhenguo Li, Qiuping Qin, Xinyi Nie, Shihua Wang
Cyclic AMP signaling controls a range of physiological processes in response to extracellular stimuli in organisms. Among the signaling cascades, cAMP, as a second messenger, is orchestrated by adenylate cyclase(biosynthesis) and cAMP phosphodiesterases (PDEs)(hydrolysis). In this study, we investigated the function of the high-affinity (PdeH) and low-affinity (PdeL) cAMP phosphodiesterase from the carcinogenic aflatoxin producing fungus Aspergillus flavus, and found that instead of PdeL, inactivation of PdeH exhibited a reduction in conidiation and sclerotia formation...
February 14, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28209731/transport-of-fungal-rab11-secretory-vesicles-involves-myosin-5-dynein-dynactin-p25-and-kinesin-1-and-is-independent-of-kinesin-3
#10
Miguel A Peñalva, Jun Zhang, Xin Xiang, Areti Pantazopoulou
Hyphal tip cells of the fungus Aspergillus nidulans are useful to study long-range intracellular traffic. Post-Golgi secretory vesicles (SVs) containing the RAB11 orthologue RabE engage myosin-5 as well as plus-end- and minus-end-directed microtubule motors, providing an experimental system to investigate the interplay between microtubule and actin motors acting on the same cargo. By exploiting the fact that depolymerization of F-actin 'unleashes' SVs focused at the apex by myosin-5 to microtubule-dependent motors, we establish that the minus-end directed transport of SVs requires the dynein/dynactin supercomplex...
February 16, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28209146/heterologous-expression-of-aspergillus-aculeatus-endo-polygalacturonase-in-pichia-pastoris-by-high-cell-density-fermentation-and-its-application-in-textile-scouring
#11
Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua, Duriya Chantasingh
BACKGROUND: Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-polygalacturonase (EndoPG) from Aspergillus aculeatus produced in Pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation. RESULTS: The recombinant EndoPG exhibited a specific activity of 1892...
February 16, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28207190/diaporthe-soft-tissue-infection-in-a-heart-transplant-patient
#12
Robert M Rakita, Kevin D O'Brien, Lori Bourassa
Infections caused by Diaporthe species are very uncommon. We describe a heart transplant recipient 14 years post transplant who developed a soft tissue fungal infection due to a Diaporthe species that responded well to surgical excision and posaconazole therapy. The Aspergillus galactomannan index was markedly elevated, and returned to normal following treatment. Solid organ transplant patients remain at risk of infection long after transplant and should be counseled about risk avoidance. This article is protected by copyright...
February 16, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28205583/flavichalasines-a-m-cytochalasan-alkaloids-from-aspergillus-flavipes
#13
Guangzheng Wei, Dongdong Tan, Chunmei Chen, Qingyi Tong, Xiao-Nian Li, Jinfeng Huang, Junjun Liu, Yongbo Xue, Jianping Wang, Zengwei Luo, Hucheng Zhu, Yonghui Zhang
Two new tetracyclic cytochalasans, flavichalasines A and B (1 and 2), three new pentacyclic cytochalasans, flavichalasines C-E (3-5), and eight new tricyclic cytochalasans, flavichalasines F-M (6-13), together with eight known analogues (14-21), were isolated from the solid culture of Aspergillus flavipes. Structures of these new compounds were elucidated on the basis of extensive spectroscopic analyses including 1D, 2D NMR and HRESIMS data. Their absolute configurations were determined by comparison of their experimental ECD with either computed ECD or experimental ECD spectrum of known compound...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28204952/a-preliminary-report-of-indigenous-fungal-isolates-from-contaminated-municipal-solid-waste-site-in-india
#14
Abhishek Kumar Awasthi, Akhilesh Kumar Pandey, Jamaluddin Khan
Municipal solid waste (MSW) containing harmful substances is a major concern in waste management and can cause adverse effects on diversity of fungi in soil. The main objective was to evaluate the fungal diversity inhabiting in the soil nearby MSW disposal site. The fungal strains were isolated in potato dextrose agar (PDA), media at temperatures 28 ± 1 °C by using standard serial dilution pour plate method, and appeared fungal colonies identified based on morphological characteristics. The overall most fungal diversity was found in soil sample collected from S5, followed by S4, S3, S1, and least in S2 site...
February 15, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28203232/cationic-net-charge-and-counter-ion-type-as-antimicrobial-activity-determinant-factors-of-short-lipopeptides
#15
Katarzyna E Greber, Malgorzata Dawgul, Wojciech Kamysz, Wieslaw Sawicki
To get a better insight into the antimicrobial potency of short cationic lipopeptides, 35 new entities were synthesized using solid phase peptide strategy. All newly obtained lipopeptides were designed to be positively charged from +1 to +4. This was achieved by introducing basic amino acid - lysine - into the lipopeptide structure and had a hydrophobic fatty acid chain attached. Lipopeptides were subjected to microbiological tests using reference strains of Gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Gram-positive bacteria: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Enterococcus faecalis, and fungi: Candida albicans, Candida tropicalis, Aspergillus brasiliensis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28203225/regulation-of-sterol-biosynthesis-in-the-human-fungal-pathogen-aspergillus-fumigatus-opportunities-for-therapeutic-development
#16
REVIEW
Sourabh Dhingra, Robert A Cramer
Sterols are a major component of eukaryotic cell membranes. For human fungal infections caused by the filamentous fungus Aspergillus fumigatus, antifungal drugs that target sterol biosynthesis and/or function remain the standard of care. Yet, an understanding of A. fumigatus sterol biosynthesis regulatory mechanisms remains an under developed therapeutic target. The critical role of sterol biosynthesis regulation and its interactions with clinically relevant azole drugs is highlighted by the basic helix loop helix (bHLH) class of transcription factors known as Sterol Regulatory Element Binding Proteins (SREBPs)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28203095/burden-and-treatment-patterns-of-invasive-fungal-infections-in-hospitalized-patients-in-the-middle-east-real-world-data-from-saudi-arabia-and-lebanon
#17
Adel F Alothman, Abdulhakeem O Althaqafi, Madonna J Matar, Rima Moghnieh, Thamer H Alenazi, Fayssal M Farahat, Shelby Corman, Caitlyn T Solem, Nirvana Raghubir, Cynthia Macahilig, Claudie Charbonneau, Jennifer M Stephens
OBJECTIVES: The objective of this study was to document the burden and treatment patterns associated with invasive fungal infections (IFIs) due to Candida and Aspergillus species in Saudi Arabia and Lebanon. METHODS: A retrospective chart review study was conducted using data recorded from 2011 to 2012 from hospitals in Saudi Arabia and Lebanon. Patients were included if they had been discharged with a diagnosis of IFI due to Candida or Aspergillus, which was culture proven or suspected based on clinical criteria...
2017: Infection and Drug Resistance
https://www.readbyqxmd.com/read/28202870/pulmonary-actinomycosis-mimicking-pulmonary-aspergilloma-and-a-brief-review-of-the-literature
#18
Yoshitsugu Higashi, Shigeki Nakamura, Nobuyuki Ashizawa, Kazuhiro Oshima, Akitaka Tanaka, Taiga Miyazaki, Koichi Izumikawa, Katsunori Yanagihara, Yoshihiro Yamamoto, Yoshitsugu Miyazaki, Hiroshi Mukae, Shigeru Kohno
Pulmonary actinomycosis is a rare pulmonary infection that often exhibits unspecific symptoms and radiological findings. We herein report a case of pulmonary actinomycosis that mimicked pulmonary aspergilloma in an immunocompetent patient.
2017: Internal Medicine
https://www.readbyqxmd.com/read/28202251/high-incidence-of-non-tuberculous-mycobacteria-positive-cultures-among-adolescent-with-cystic-fibrosis
#19
Zoé Cavalli, Quitterie Reynaud, Romain Bricca, Raphaële Nove-Josserand, Stéphane Durupt, Philippe Reix, Marie Perceval, Michèle Pérouse de Montclos, Gérard Lina, Isabelle Durieu
BACKGROUND: We evaluated the prevalence of non-tuberculous mycobacteria (NTM)-positive cultures among our cystic fibrosis (CF) center patients, reviewed risk factors for NTM positivity, and determined its impact on lung function evolution. METHODS: From 2009 to 2014, CF adults and children attending the CF center of Lyon (France) and having at least one positive NTM isolate were included. Each case was matched by age and gender with two CF patients with no NTM isolate (controls)...
February 12, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/28198513/sybr-safe-tm-efficiently-replaces-ethidium-bromide-in-aspergillus-fumigatus-gene-disruption
#20
H M S Canela, L A Takami, M E S Ferreira
Invasive aspergillosis is a disease responsible for high mortality rates, caused mainly by Aspergillus fumigatus. The available drugs are limited and this disease continues to occur at an unacceptable frequency. Gene disruption is essential in the search for new drug targets. An efficient protocol for A. fumigatus gene disruption was described but it requires ethidium bromide, a genotoxic agent, for DNA staining. Therefore, the present study tested SYBR safe(TM), a non-genotoxic DNA stain, in A. fumigatus gene disruption protocol...
February 8, 2017: Genetics and Molecular Research: GMR
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