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https://www.readbyqxmd.com/read/28527994/purification-and-characterization-of-a-protease-resistant-phytase-of-aspergillus-oryzae-sbs50-whose-properties-make-it-exceptionally-useful-as-a-feed-supplement
#1
Sapna, Bijender Singh
An extracellular phytase of Aspergillus oryzae SBS50 was purified to homogeneity using ammonium sulphate precipitation, ion-exchange and gel filtration chromatography. Purified phytase has a monomeric molecular mass of ∼80kDa exhibiting its optimal activity at pH 5.0 and 50°C with a T 1/2 of 300min at 50°C. Phytase of A. oryzae displayed broad substrate specificity with Vmax and Km values of 58.82μmol/ml/min and 1.14mM, respectively, for calcium phytate. Purity and homogeneity of the phytase was confirmed by high performance liquid chromatography and MALDI-TOF analysis revealed the identification of a peptide showing homology with acid phosphatase of Aspergillus oryzae RIB40...
May 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28527583/molecular-characterization-of-aspergillus-fumigatus-tcsc-a-characteristic-type-iii-hybrid-histidine-kinase-of-filamentous-fungi-harboring-six-hamp-domains
#2
Anja Spadinger, Frank Ebel
The type III hybrid histidine kinase (HHK) TcsC enables the pathogenic mold Aspergillus fumigatus to thrive under hyperosmotic conditions. It is, moreover, of particular interest, since it is the target of certain antifungal agents, such as fludioxonil. This study was aimed at a functional characterization of the domains that constitute the sensing and the kinase module of TcsC. The sensing module consists of six HAMP domains, an architecture that is commonly found in type III HHKs of filamentous fungi. To dissect the functional role of the individual domains, we have analyzed a set of truncated derivatives of TcsC with respect to their impact on fungal growth and their ability to respond to hyperosmotic stress and fludioxonil...
May 11, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28526521/coexistence-of-aspergilloma-and-pulmonary-hydatid-cyst-in-an-immunocompetent-individual
#3
F Aala, H Badali, S Hashemi Fesharaki, M Boroumand, M Sotoudeh Anvari, H Davari, S Agha Kuchak Afshari, S Khodavaisy
Echinococcosis is a zoonotic disease caused by Echinococcus granulosus sensu lato. The liver and lungs are the most commonly sites of infections, but involvements of other organs were also observed. Recently, the coinfection of pulmonary hydatid cyst with aspergilloma has been reported in the literature. Herein, we report a successful treatment of coinfection of cystic echinoccosis with aspergilloma due to Aspergillus flavus in a 34-year-old female. In vitro antifungal susceptibility tests revealed that the MIC values for antifungals employed in this case were posaconazole (0...
May 16, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28526275/increasing-total-serum-ige-allergic-bronchopulmonary-aspergillosis-and-lung-function-in-cystic-fibrosis
#4
Florian Gothe, Matthias Kappler, Matthias Griese
BACKGROUND: Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity disorder contributing to lung disease in cystic fibrosis (CF) and challenging to diagnose. OBJECTIVE: This study analyzed the predictive value of increasing total IgE (t-IgE) levels in a CF cohort alongside with clinical and serologic data. METHODS: A total of 387 children and young adults were followed from 2000 to 2006 and retrospectively classified into 6 groups...
May 16, 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/28525761/impact-of-bioactive-packaging-systems-based-on-evoh-films-and-essential-oils-in-the-control-of-aflatoxigenic-fungi-and-aflatoxin-production-in-maize
#5
Eva M Mateo, José V Gómez, Irene Domínguez, Jose V Gimeno-Adelantado, Rufino Mateo-Castro, Rafael Gavara, Misericordia Jiménez
Aspergillus flavus and A. parasiticus are the most common fungal species associated with aflatoxin (AF) contamination of cereals, especially maize, and other agricultural commodities. AFB1, the most frequent and toxic metabolite, is a powerful hepatotoxic, teratogenic and mutagenic compound. Effective strategies to control these fungal species and AFs in food and feed are required. Active packaging film containing essential oils (EO) is one of the most innovative food packaging concepts. In this study, ethylene-vinyl alcohol (EVOH) copolymer films incorporating EO from Origanum vulgare (ORE), Cinnamomum zeylanicum (CIN) or their major active constituents, carvacrol (CAR) and cinnamaldehyde (CINHO), respectively, were developed and assayed to control growth of A...
May 11, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28525619/epidemiology-and-clinical-characteristics-of-invasive-mould-infections-a-multicenter-retrospective-analysis-in-five-asian-countries
#6
R Porpon, Y C Chen, A Chakrabarti, R Y Li, R M Shivaprakash, J Yu, H C Kung, S Watcharananan, A L Tan, S E Saffari, B H Tan
Formal, large-scale, multicenter studies of invasive mould infection (IMI) in Asia are rare. This 1-year, retrospective study was designed to assess the incidence and clinical determinants of IMI in centers in five countries (Thailand, Taiwan, Singapore, China, India). Patients treated in a single year (2012) were identified through discharge diagnoses, microbiology, and histopathology logs, and entered based on published definitions of IMI. A total of 155 cases were included (median age 54 years; 47.7% male)...
May 19, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28525576/profiling-of-volatile-organic-compounds-produced-by-clinical-aspergillus-isolates-using-gas-chromatography-mass-spectrometry
#7
M G Gerritsen, P Brinkman, N Escobar, L D Bos, K de Heer, M Meijer, H-G Janssen, H de Cock, H A B Wösten, C E Visser, M H J van Oers, P J Sterk
Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography-mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro...
May 18, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28525266/gliotoxin-biosynthesis-structure-mechanism-and-metal-promiscuity-of-carboxypeptidase-glij
#8
Antoine Marion, Michael Groll, Daniel H Scharf, Kirstin Scherlach, Manuel Glaser, Holger Sievers, Michael Schuster, Christian Hertweck, Axel A Brakhage, Iris Antes, Eva M Huber
The formation of glutathione (GSH) conjugates, best known from the detoxification of xenobiotics, is a widespread strategy to incorporate sulfur into biomolecules. The biosynthesis of gliotoxin, a virulence factor of the human pathogenic fungus Aspergillus fumigatus, involves attachment of two GSH molecules and their sequential decomposition to yield two reactive thiol groups. The degradation of the GSH moieties requires the activity of the Cys-Gly carboxypeptidase GliJ, for which we describe the X-ray structure here...
May 19, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28524886/fungal-keratitis-in-the-republic-of-ireland
#9
S Farrell, E McElnea, S Moran, S Knowles, C C Murphy
PurposeFungal keratitis is relatively rare in temperate climates with only one previous population-based study, which reported a minimum annual incidence of 0.32 per million population in the United Kingdom. We performed a nationwide study in the Republic of Ireland of cases over a 6-year period to establish the incidence, risk factors, treatment, and outcomes of fungal keratitis.Patients and methodsAll corneal scraping specimens positive for fungal species over a 6-year period were identified at the microbiology departments of the seven main ophthalmology units in the Republic of Ireland...
May 19, 2017: Eye
https://www.readbyqxmd.com/read/28523137/complex-multimodality-central-airway-management-of-aspergillus-pseudomembranous-tracheobronchitis
#10
Samjot Singh Dhillon, Marwan Saoud, Kassem Harris
A 69-year-old woman developed central airway obstruction due to invasive Aspergillus infection resulting in pseudomembranous tracheobronchitis (PTB). Several challenges were encountered in the airway management of this patient including her having relatively smaller airways which were more prone to obstruction by pseudomembranes and made airway interventions difficult. The patient had clinical deterioration in spite of antifungal therapy and bronchoscopic debridement. The multimodality airway techniques included the use of smaller biliary balloons for dilatation, using a hybrid stent to slowly dilate and maintain patency of right main stem and finally the insertion of right secondary carina peripheral Y stent, which resulted in clinical improvement and allowed time for antifungal therapy to take effect...
April 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28521816/biochemical-characterization-of-the-xylan-hydrolysis-profile-of-the-extracellular-endo-xylanase-from-geobacillus-thermodenitrificans-t12
#11
Martinus J A Daas, Patricia Murciano Martínez, Antonius H P van de Weijer, John van der Oost, Willem M de Vos, Mirjam A Kabel, Richard van Kranenburg
BACKGROUND: Endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. Hemicellulose degradation by Geobacillus spp. is facilitated by the hemicellulose utilization (HUS) locus that is present in most strains belonging to this genus. As part of the HUS locus, the xynA gene encoding an extracellular endo-xylanase is one of the few secreted enzymes and considered to be the key enzyme to initiate hemicellulose degradation. Several Geobacillus endo-xylanases have been characterized for their optimum temperature, optimum pH and generation of degradation products...
May 18, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28520936/impacts-of-amino-acid-substitutions-in-fungal-cytochrome-p450-monooxygenase-cyp57b3-on-the-effective-production-of-3-hydroxygenistein
#12
Mayumi Hatakeyama, Takuya Kitaoka, Hirofumi Ichinose
Aspergillus oryzae cytochrome P450 monooxygenase (CYP57B3) is capable of catalyzing hydroxylation of genistein to produce 3΄-hydroxygenistein. Because hydroxylated derivatives of genistein, including 3΄-hydroxygenistein, exhibit various pharmacological activities, CYP57B3 would potentially be useful as a biocatalyst in the pharmaceutical field. We therefore performed random mutagenesis of CYP57B3 to improve its catalytic activities for genistein. Random mutations were introduced by error-prone PCR into CYP57B3, resulting in construction of a library of mutants...
May 18, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28520596/fungal-keratitis-associated-with-airborne-organic-debris-and-soft-contacts-lenses-case-reports-and-review-of-the-literature
#13
Michael J Hodkin, Ryan C Gustus
OBJECTIVES: To report two cases of fungal keratitis in soft contact lens wearers who were exposed to environmental mold. METHODS: Case reports, with review of the relevant literature. RESULTS: Two otherwise healthy middle-aged women who were soft contact lens wearers developed fungal keratitis about 1 week after heavy exposure to environmental mold. Culture results showed Aspergillus from one patient and the unusual combination of Trichoderma and Penicillium from the other...
May 16, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28517364/a-cryptic-pigment-biosynthetic-pathway-uncovered-by-heterologous-expression-is-essential-for-conidial-development-in-pestalotiopsis-fici
#14
Peng Zhang, Xiuna Wang, Aili Fan, Yanjing Zheng, Xingzhong Liu, Shihua Wang, Huixi Zou, Berl R Oakley, Nancy P Keller, Wen-Bing Yin
Spore pigmentation is very common in the fungal kingdom. The best studied pigment in fungi is melanin which coats the surface of single cell spores. What and how pigments function in a fungal species with multiple cell conidia is poorly understood. Here, we identified and deleted a polyketide synthase (PKS) gene PfmaE and showed that it is essential for multicellular conidial pigmentation and development in a plant endophytic fungus, Pestalotiopsis fici. To further characterize the melanin pathway, we utilized an advanced Aspergillus nidulans heterologous system for the expression of the PKS PfmaE and the Pfma gene cluster...
May 18, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28516246/aspergillus-species-in-bronchiectasis-challenges-in-the-cystic-fibrosis-and-non-cystic-fibrosis-airways
#15
REVIEW
Sanjay H Chotirmall, Maria Teresa Martin-Gomez
Bronchiectasis is a chronic irreversible airway abnormality associated with infectious agents that either cause or superinfect the airways. While the role of bacteria is well studied, much remains to be determined about fungi in both cystic fibrosis- and non-cystic fibrosis-related bronchiectasis. The airway is constantly exposed to inhaled ambient moulds of which Aspergillus represent the most ubiquitous. In a normal healthy host, this situation is of little consequence. The presence of anatomical or immunological abnormalities such as those in bronchiectasis leads to a range of fungal-related pathologies from asymptomatic airway colonization to fungal sensitization, allergic bronchopulmonary aspergillosis or chronic pulmonary aspergillosis...
May 17, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28516145/zinc-nickel-and-cobalt-ions-removal-from-aqueous-solution-and-plating-plant-wastewater-by-modified-aspergillus-flavus-biomass-a-dataset
#16
Rauf Foroutan, Hossein Esmaeili, Seyedehmasomeh Derakhshandeh Rishehri, Farzaneh Sadeghzadeh, Seyedehroghayeh Mirahmadi, Malihe Kosarifard, Bahman Ramavandi
The biomass of Aspergillus flavus was modified by calcium chloride to achieve a bioadsorbent for treating nickel, cobalt, and zinc ions from aqueous solutions. The information of pH, bioadsorbent dose, contact time, and temperature effect on the removal efficiency are presented. The data of Freundlich and Langmuir isotherm and pseudo-first-order and pseudo-second-order kinetic models are also depicted. The data showed that the maximum bioadsorption capacity of nickel, cobalt, and zinc ions is 32.26, 31.06 and 27...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28515718/fungal-diversity-in-field-mold-damaged-soybean-fruits-and-pathogenicity-identification-based-on-high-throughput-rdna-sequencing
#17
Jiang Liu, Jun-Cai Deng, Cai-Qiong Yang, Ni Huang, Xiao-Li Chang, Jing Zhang, Feng Yang, Wei-Guo Liu, Xiao-Chun Wang, Tai-Wen Yong, Jun-Bo Du, Kai Shu, Wen-Yu Yang
Continuous rain and an abnormally wet climate during harvest can easily lead to soybean plants being damaged by field mold (FM), which can reduce seed yield and quality. However, to date, the underlying pathogen and its resistance mechanism have remained unclear. The objective of the present study was to investigate the fungal diversity of various soybean varieties and to identify and confirm the FM pathogenic fungi. A total of 62,382 fungal ITS1 sequences clustered into 164 operational taxonomic units (OTUs) with 97% sequence similarity; 69 taxa were recovered from the samples by internal transcribed spacer (ITS) region sequencing...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28515264/functional-characterization-of-the-copper-transcription-factor-mrpa%C3%A2-from-aspergillus-fumigatus
#18
Yong-Sung Park, Tae-Hyoung Kim, Cheol-Won Yun
Although copper functions as a cofactor in many physiological processes, copper overload leads to harmful effects in living cells. Thus, copper homeostasis is tightly regulated. However, detailed copper metabolic pathways have not yet been identified in filamentous fungi. In this report, we investigated the copper transcription factor AfMac1 (Aspergillus fumigatus Mac1 homologue), and identified its regulatory mechanism in A. fumigatus AfMac1 has domains homologous to the DNA- and copper-binding domains of Mac1 from Saccharomyces cerevisiae , and AfMac1 efficiently complemented Mac1 in S...
May 17, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28515220/culture-based-methods-and-molecular-tools-for-azole-resistant-aspergillus-fumigatus-detection-in-a-belgian-university-hospital
#19
I Montesinos, M A Argudín, M Hites, F Ahajjam, M Dodémont, C Dagyaran, M Bakkali, I Etienne, F Jacobs, C Knoop, S Patteet, K Lagrou
Azole-resistant Aspergillus fumigatus is an increasing worldwide problem with major clinical implications. Surveillance is warranted to guide clinicians to provide optimal treatment to patients. To investigate azole resistance in clinical Aspergillus isolates in our institution, a Belgian university hospital, we conducted a laboratory-based surveillance between June 2015 and October 2016. Two different approaches were used: a prospective culture-based surveillance using VIPcheck™ on unselected A. fumigatus (n=109 patients, including 19 patients with proven or probable IA), followed by molecular detection of mutations conferring azole resistance and a retrospective detection of azole-resistant A...
May 17, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28515217/an-analytical-and-clinical-evaluation-of-the-pathonostics-aspergenius%C3%A2-assay-for-the-detection-of-invasive-aspergillosis-and-resistance-to-azole-antifungal-drugs-direct-from-plasma-samples
#20
P Lewis White, Raquel B Posso, Rosemary A Barnes
With the proposal to include Aspergillus PCR in the revised EORTC/MSG definitions for fungal disease, commercially manufactured assays may be required to provide standardisation and accessibility. The PathoNostics AsperGenius® assay represents one such test that has the ability to detect a range of Aspergillus species and azole-resistance in A. fumigatus Performance has been validated when testing BAL and serum specimens, but recent evidence suggests that testing plasma may enhance sensitivity over serum. It was decided to evaluate the analytical and clinical performance of the PathoNostics AsperGenius® assay when testing plasma...
May 17, 2017: Journal of Clinical Microbiology
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