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https://www.readbyqxmd.com/read/28726315/biomineralization-of-gold-by-mucor-plumbeus-the-progress-in-understanding-the-mechanism-of-nanoparticles-formation
#1
Irena Maliszewska, Włodzimierz Tylus, Izabela Pawlaczyk-Graja, Wojciech Pusz, Anna Baturo-Cieśniewska, Jacek Chęcmanowski, Bogdan Szczygieł
This contribution describes the deposition of gold nanoparticles by microbial reduction of Au(III) ions using the mycelium of Mucor plumbeus. Biosorption as the major mechanism of Au(III) ions binding by the fungal cells and the reduction of them to the form of Au(0) on/in the cell wall, followed by the transportation of the synthesized gold nanoparticles to the cytoplasm, is postulated. The probable mechanism behind the reduction of Au(III) ions is discussed, leading to the conclusion that this process is non enzymatic one...
July 20, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28718996/production-of-ligninolytic-enzymes-and-some-diffusible-antifungal-compounds-by-white-rot-fungi-using-potato-solid-wastes-as-the-sole-nutrient-source
#2
H Schalchli, E Hormazábal, O Rubilar, G Briceño, A Mutis, G J Zocolo, C Diez
AIMS: The aim of this study was to evaluate the synthesis of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using peels or discarded potato as the sole nutrient source. METHODS AND RESULTS: The strain Trametes hirsuta Ru-513 has been highlighted for its laccase activity (595 ± 33 U l(-1) ), which is able to decolourise 87% of an anthraquinone dye using potato peels as the sole nutritional support. A native polyacrylamide gel of laccase proteins showed the presence of two isoenzymes, corresponding to proteins of 56 and 67 kDa, which were detected by SDS-PAGE...
July 18, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28713749/mucormycosis-of-the-frontal-sinus-a-rare-case-report-and-review
#3
Nanda Kishore Sahoo, Vishal Kulkarni, Amit K Bhandari, Arun Kumar
Mucormycosis of the frontal sinus are rarely seen in day to day clinical practice. Although this fungus is commonly found in the environment, the disease is usually prevented by the immune system and is hence rare. Well-recognized risk factors for the disease include diabetes mellitus, leukemia, aplastic anemia, myelodysplastic syndrome, blood dyscrasias, and immunosuppressive therapy in organ transplantation, renal disease, sepsis, and severe burns. The disease is primarily found in those who are immunocompromised, but it may also manifest in immuno competent persons...
January 2017: Annals of Maxillofacial Surgery
https://www.readbyqxmd.com/read/28707783/aggressive-necrotizing-pseudomonal-sinonasal-infections
#4
Edward C Kuan, Bobby A Tajudeen, Kevin C Welch, Rakesh K Chandra, Ben J Glasgow, Jeffrey D Suh
BACKGROUND: Pseudomonas aeruginosa is a gram-negative bacterium frequently implicated in recalcitrant sinonasal infections, especially in immunocompromised hosts. We report 6 cases of rapidly progressive pseudomonal acute rhinosinusitis producing tissue necrosis and, in certain cases, cranial nerve palsies. METHODS: Retrospective review of 6 patients with aggressive necrotizing sinonasal infections treated at 4 tertiary academic medical centers with sinonasal cultures growing P...
July 14, 2017: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/28705753/invasive-fungal-infections-secondary-to-traumatic-injury
#5
REVIEW
Ryan Kronen, Stephen Y Liang, Grant Bochicchio, Kelly Bochicchio, William G Powderly, Andrej Spec
Invasive fungal infection (IFI) is a rare but serious complication of traumatic injury. The purpose of this article is to review the epidemiology, natural history, mycology, risk factors, diagnosis, treatment, and outcomes associated with post-traumatic IFI in military and civilian populations. The epidemiology of post-traumatic IFI is poorly characterized, but incidence appears to be rising. Patients often suffer from severe injuries and require extensive medical interventions. Fungi belonging to the order Mucorales are responsible for most post-traumatic IFI in both civilian and military populations...
July 10, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28705685/-fungal-sinusitis
#6
REVIEW
E Lafont, C Aguilar, P Vironneau, R Kania, A Alanio, S Poirée, O Lortholary, F Lanternier
Although sinusitis affects about 20 % of the population, fungal sinusitis is rare. Aspergillus sp. are most frequently implicated. Fungal sinusitis represents a wide spectrum of disorders, including acute or chronic and invasive or non-invasive forms. Invasive fungal sinusitis may develop in an immunocompromised or diabetic patient, whereas non-invasive fungal sinusitis should be considered in a chronic situation, resistant to antibiotics in immunocompetent patients. Allergic fungal sinusitis is related to hypersensitivity of the host to the fungus...
July 10, 2017: Revue des Maladies Respiratoires
https://www.readbyqxmd.com/read/28702763/pharmacokinetics-of-antifungal-drugs-practical-implications-for-optimized-treatment-of-patients
#7
REVIEW
Romuald Bellmann, Piotr Smuszkiewicz
INTRODUCTION: Because of the high mortality of invasive fungal infections (IFIs), appropriate exposure to antifungals appears to be crucial for therapeutic efficacy and safety. MATERIALS AND METHODS: This review summarises published pharmacokinetic data on systemically administered antifungals focusing on co-morbidities, target-site penetration, and combination antifungal therapy. CONCLUSIONS AND DISCUSSION: Amphotericin B is eliminated unchanged via urine and faeces...
July 12, 2017: Infection
https://www.readbyqxmd.com/read/28701756/seed-metabolome-analysis-of-a-transgenic-rice-line-expressing-cholera-toxin-b-subunit
#8
Takumi Ogawa, Koji Kashima, Yoshikazu Yuki, Mio Mejima, Shiho Kurokawa, Masaharu Kuroda, Atsushi Okazawa, Hiroshi Kiyono, Daisaku Ohta
Plant-based human vaccines have been actively developed in recent years, and rice (Oryza sativa L.) is one of the best candidate crops for their production and delivery. By expressing a modified cholera toxin B (CTB) subunit, we previously developed MucoRice-CTB, a rice-based vaccine against cholera, which is caused by infection of the intestine with the bacteria Vibrio cholerae. MucoRice-CTB lines have been extensively characterized by whole-genome sequencing and proteome analyses to evaluate the mutation profiles and proteome status, respectively...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28698667/phylogenetic-analysis-reveals-two-genotypes-of-the-emerging-fungus-mucor-indicus-an-opportunistic-human-pathogen-in-immunocompromised-patients
#9
Saad J Taj-Aldeen, Muna Almaslamani, Bart Theelen, Teun Boekhout
Mucormycosis is a rare fungal infection caused by Mucor indicus. Phylogenetic analysis of many M. indicus isolates, mainly sampled from different clinical and environmental specimens collected worldwide, revealed two genotypes, I and II, based on ITS and D1/D2 LSU rDNA sequences. A retrospective review of the literature revealed 13 cases. Eight (76.9%) patients had disseminated infections, and the overall mortality rate was 30.7%. A pulmonary infection caused by M. indicus genotype I in a liver transplant recipient was disseminated to include the skin and was successfully treated with liposomal amphotericin B and aggressive surgery...
July 12, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28687045/sageretia-thea-osbeck-modulated-biosynthesis-of-nio-nanoparticles-and-their-in-vitro-pharmacognostic-antioxidant-and-cytotoxic-potential
#10
Ali Talha Khalil, Muhammad Ovais, Ikram Ullah, Muhammad Ali, Zabta Khan Shinwari, Dilawar Hassan, Malik Maaza
NiO nanoparticles are biosynthesized using Sageretia thea (Osbeck.) aqueous leave extracts and their biological activities are reported. Nanoparticles (∼18 nm) were characterized through XRD, ATR-FTIR, EDS, SAED, HR-SEM/TEM and Raman spectroscopy. Antibacterial activity was investigated against six pathogenic bacterial strains (gram positive and gram negative) and their corresponding minimum inhibitory concentrations (MICs) were calculated. UV-exposed nanoparticles were investigated to have reduced MICs relative to the NiO nanoparticles have not been exposed to UV...
July 7, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28685375/higher-in-vitro-proliferation-rate-of-rhizopus-oryzae-in-blood-of-diabetic-individuals-in-chronic-glycaemic-control-compared-with-non-diabetic-individuals
#11
Grace Salazar-Tamayo, Luis E López-Jácome, Jesús Resendiz-Sanchez, Rafael Franco-Cendejas, Patricia Rodriguez-Zulueta, Dora E Corzo-León
Metabolic control improves outcomes associated with mucormycosis. The aim of this study was to compare the in vitro proliferation of Rhizopus oryzae in blood of individuals with and without diabetes at different glycaemic levels. Ninety-five individuals were included. Blood samples from each participant were incubated with sporangiospores of R. oryzae. The germination, filamentation and growth of R. oryzae were compared at different time points. Four groups were defined, one without (group A, n = 30) and three with diabetes: group B (HbA1c ≤7%, N = 24), group C (HbA1c 7...
July 6, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28681286/bioremoval-of-arsenic-v-from-aqueous-solutions-by-chemically-modified-fungal-biomass
#12
J F Cárdenas-González, I Acosta-Rodríguez, Y Téran-Figueroa, A S Rodríguez-Pérez
The biosorption of arsenic (V) on nine chemically modified biomasses (with iron oxide coated) of mycelia fungi: Aspergillus flavus III, IV and V, Aspergillus fumigatus I-II, Paecilomyces sp., Cladosporium sp., Mucor sp-1 and 2 was studied in this work. This study provides evidence that the biomasses of the fungi A. flavus, IV, III and V, Paecilomyces sp., and A. fumigatus I were very efficient at removing 1 mg/L of the metal in solution, using atomic absorption spectroscopy (AAS), achieving the following percentage of removals: 97...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28680526/lateral-flow-assays-for-the-diagnosis-of-invasive-aspergillosis-current-status
#13
REVIEW
Sven Heldt, Martin Hoenigl
PURPOSE OF REVIEW: Diagnosis during early stages of invasive aspergillosis (IA) and targeted antifungal treatment has the potential to improve survival significantly. Despite advances in the diagnostic arsenal, invasive mold infections remain difficult to diagnose-especially at early stages before typical radiological signs develop. Varying availability and time-to-results are important limitations of current approved biomarkers and molecular assays for diagnosis of IA. Here, we will give an update on the Aspergillus-specific lateral-flow device (LFD) test...
2017: Current Fungal Infection Reports
https://www.readbyqxmd.com/read/28667347/clinical-analysis-of-diabetic-combined-pulmonary-mucormycosis
#14
Yu-Hong Li, Peng Sun, Jian-Chao Guo
OBJECTIVE: To improve the understanding of diabetic combined pulmonary mucormycosis. METHODS AND RESULTS: The clinical data of twelve patients diagnosed as diabetic combined pulmonary mucormycosis were analyzed by the clinical manifestations, imaging features, diagnosis, treatment and prognosis of this disease. Patients from January 1999 to June 2015 in our hospital had poor blood glucose control and varying degrees of diabetic chronic complications with an on average of diabetes 8...
June 30, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28659561/detection-of-fungi-from-an-indoor-environment-using-loop-mediated-isothermal-amplification-lamp-method
#15
Takako Nakayama, Takashi Yamazaki, Ayaka Yo, Kazuya Tone, Mohamed Mahdi Alshahni, Ryuichi Fujisaki, Koichi Makimura
 Loop-mediated isothermal amplification (LAMP) is a useful DNA detection method with high specificity and sensitivity. The LAMP reaction is carried out within a short time at a constant temperature without the need for thermal cycling. We developed a LAMP primer set for detecting a wide range of fungi by aligning the sequences of the large subunit ribosomal RNA gene of Candida albicans (Ascomycota), Cryptococcus neoformans (Basidiomycota), and Mucor racemosus (Mucorales). The threshold of C. albicans rDNA as template with our LAMP primer set was in the range of 10-100 copies per a reaction...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28658779/sclerosing-mediastinitis-presenting-as-complete-heart-block
#16
Reena Bharadwaj, Manoj Gopal Madakshira, Prashant Bharadwaj, Hardeep Singh Sidhu
Sclerosing Mediastinitis (SM) is a rare condition which is characterized by the presence of dense fibrosis which infiltrates and encroaches upon various structures in the mediastinal cavity. Depending on the extent and the predominant organ of involvement, the patient presents with symptomatology of dysphagia, superior vena cava compression syndrome or dyspnoea. However, the involvement of the heart is rarely seen. Aetiologies of SM are several, with infections being the most common. We discuss a case of SM involving the oesophagus, descending aorta, hilum of lungs and the heart secondary to mucormycosis in an immunocompetent male...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28658765/prevalence-of-lung-lesions-at-autopsy-a-histopathological-study
#17
Pratima Khare, Renu Gupta, Mukta Ahuja, Nupur Khare, Swapnil Agarwal, Deepti Bansal
INTRODUCTION: Autopsies are carried out to establish the identity, cause of death, time of death, and ante-mortem or post-mortem nature of death. These help in establishing the cause of death and ascertain the disease process which led to death. AIM: To study the prevalence and pattern of lung diseases in medicolegal autopsies, confirmed by histopathological examination. MATERIALS AND METHODS: This retrospective study was carried out in the Department of Pathology...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28652241/prophylactic-treatment-with-vt-1161-protects-immunosuppressed-mice-from-rhizopus-arrhizus-var-arrhizus-infection
#18
Teclegiorgis Gebremariam, Sondus Alkhazraji, Lin Lin, Nathan P Wiederhold, Edward P Garvey, William J Hoekstra, Robert J Schotzinger, Thomas F Patterson, Scott G Filler, Ashraf S Ibrahim
We compared prophylactic or continuous therapy of the investigational drug VT-1161 to posaconazole in treating murine mucormycosis due to Rhizopus arrhizus var. arrhizus In the prophylaxis studies, only VT-1161 resulted in improved survival and lowered tissue fungal burden of immunosuppressed infected mice. In the continuous therapy, VT-1161 outperformed posaconazole in prolonging mice survival time despite their comparable effect in lowering tissue fungal burden. These results support the further development of VT-1161 against mucormycosis...
June 26, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28646276/rhizopus-arrhizus-invasive-infection-due-to-self-inflicted-scratch-injuries-in-a-diabetic-patient-with-non-ketotic-acidosis
#19
Luis Buzón Martín, Miguel Ángel Morán Rodríguez, Nicole Mercier, Maria Ortega Lafont, Eva Ojeda Fernández, Ana Ruiz de la Parte, Miguel Estefanía
Mucormycosis is a rare infection caused by members included in the subphylum Mucoromycotina. Characterized by the histopathological hallmark of angioinvasion, these infections affect most often patients with certain underlying conditions carrying immunosuppression (haematological neoplasias, diabetic ketoacidosis and other forms of acidosis, and iron overload) or immunocompetent patients with traumatic mucocutaneous barriers breakdown and direct inoculation of the mould. A case is presented in which a rare underlying condition (non-ketotic acidosis) and a rare cause of cutaneous injuries collide...
June 23, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28639065/nosocomial-rhinocerebral-mucormycosis-two-cases-with-a-temporal-relationship
#20
Justo Sánchez-Gil, Emilio Guirao-Arrabal, Ginés David Parra-García, María Del Pilar Luzón-García, Maria Fe Bautista-Marín, Matías Barayobre-Barayobre, Andrés Fontalba-Navas
Mucormycosis is an unusual fungal infection that usually affects immunosuppressed patients. Small outbreaks of mucormycosis have been previously reported. We present two clinical cases of fatal rhinocerebral mucormycosis with a close temporal relationship between them and a possible nosocomial transmission: case 1 was a 75-year-old male with diabetes and COPD, treated with antibiotics and systemic corticosteroids, who developed rhinocerebral mucormycosis. Case 2 was an 88-year-old woman who was treated with systemic antibiotics and corticosteroids and developed the same infection after insertion of a nasogastric tube...
June 21, 2017: Mycopathologia
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