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https://www.readbyqxmd.com/read/27907860/co-delivery-of-vp-16-and-bcl-2-targeted-antisense-on-peg-grafted-omwcnts-for-synergistic-in-vitro-anti-cancer-effects-in-non-small-and-small-cell-lung-cancer
#1
Zbynek Heger, Hana Polanska, Sona Krizkova, Jan Balvan, Martina Raudenska, Simona Dostalova, Amitava Moulick, Michal Masarik, Vojtech Adam
Present study describes the preparation of a polyethylene glycol-grafted oxidized multi-walled carbon nanotubes (oMWCNTs-PEG) hybrid nanosystem as a carrier of etoposide (VP-16) and Bcl-2 phosphorothioate antisense deoxyoligonucleotides (Aso) to achieve a superior cytostastic efficacy in non-small and small cell lung cancer in vitro. We have demonstrated that the adsorption of hydrophobic VP-16 and Bcl-2 Aso results in a stable nanotransporter exhibiting good dispersion with excellent release profiles (both, in pH 7...
November 19, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27906431/overexpression-of-microrna-365-inhibits-breast-cancer-cell-growth-and-chemo-resistance-through-galnt4
#2
J Zhang, Z Zhang, Q Wang, X-J Xing, Y Zhao
OBJECTIVE: A role of microRNA-365 (miR-365) in the carcinogenesis of breast cancer remains undetermined. In the current study, we addressed this question. PATIENTS AND METHODS: We examined the levels of miR-365 in breast cancer tissue, compared to the paired adjacent non-tumor breast tissue from the patients. We also examined the effects of miR-365 modification on the breast cancer growth and sensitivity to Fluorouracil, as well as the underlying mechanisms. RESULTS: The miR-365 levels in breast cancer tissue were significantly lower than those in control normal breast tissues...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27904776/microrna-195-suppresses-colorectal-cancer-cells-proliferation-via-targeting-fgf2-and-regulating-wnt-%C3%AE-catenin-pathway
#3
Xianxiang Zhang, Ji Xu, Tao Jiang, Guangwei Liu, Dongsheng Wang, Yun Lu
Colorectal cancer (CRC) is a prevalent cancer with high mortality worldwide. This study was aimed to explore the functional effects of microRNA-195 (miR-195) on CRC cells and the underling mechanism involved. quantitative PCR (qPCR) was performed to monitor the expression of miR-195 in CRC tissues and cell lines. SW480 and SW620 cells were transfected with either miR-195 mimic or antisense oligonucleotides (ASO) of miR-195. Then cell viability, cell cycle and the expressions of CyclinB1, CyclinD1 and Cyclin-Dependent Kinase 2 (CDK2) were respectively detected by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyltetrazolium bromide (MTT), flow cytometry, qPCR and Western blot...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27904706/the-central-role-of-hotair-in-the-malignancy-of-cd44-human-hypopharyngeal-carcinoma-cells
#4
Hongxia Cheng, Xinhua Cui, Ying Guo, Lintao Gu, Yaning Wang, Qirong Wang, Hui Liang
Human hypopharyngeal carcinoma is one of the most common malignant tumors. CD44 could serve as a molecular marker to screen for cancer stem cells (CSCs) in hypopharyngeal cancer. The aim of this study was to identify the role of HOX transcript antisense RNA (HOTAIR) on cell proliferation and invasion in CD44+ FADU cells (human hypopharyngeal carcinoma cells). We also explored the underlying mechanism contributing to HOTAIR's observed effects. CD44+ FADU cells were sorted and purified by flow cytometry and infected with lentivirus stably expressing HOTAIR shRNA...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27900563/lncrna-hotair-promotes-cisplatin-resistance-in-gastric-cancer-by-targeting-mir-126-to-activate-the-pi3k-akt-mrp1-genes
#5
Jin Yan, Yini Dang, Shiyu Liu, Yifeng Zhang, Guoxin Zhang
Altered expression of long noncoding RNAs (lncRNAs) has shown to associate with human cancer development and progression and drug resistance. LncRNA HOX antisense intergenic RNA (HOTAIR) regulates chromatin state and highly expressed in various human cancers. This study analyzed HOTAIR expression in gastric cancer cells and tissues and then assessed the effects of HOTAIR on modulation of gastric cancer cell sensitivity to cisplatin and the underlying molecular events. The data showed that HOTAIR was significantly upregulated in cisplatin-resistant gastric cancer cells and tissues compared with control cells and noncancerous gastric tissues...
November 30, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27899596/chitars-3-1-the-enhanced-chimeric-transcripts-and-rna-seq-database-matched-with-protein-protein-interactions
#6
Alessandro Gorohovski, Somnath Tagore, Vikrant Palande, Assaf Malka, Dorith Raviv-Shay, Milana Frenkel-Morgenstern
Discovery of chimeric RNAs, which are produced by chromosomal translocations as well as the joining of exons from different genes by trans-splicing, has added a new level of complexity to our study and understanding of the transcriptome. The enhanced ChiTaRS-3.1 database (http://chitars.md.biu.ac.il) is designed to make widely accessible a wealth of mined data on chimeric RNAs, with easy-to-use analytical tools built-in. The database comprises 34 922: chimeric transcripts along with 11 714: cancer breakpoints...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27897239/expression-level-and-clinical-significance-of-hox-transcript-antisense-intergenic-rna-in-cervical-cancer-a-meta-analysis
#7
Shasha Liu, Min Zhang, Pengpeng Qu
The long noncoding RNA HOX transcript antisense intergenic RNA (HOTAIR) is associated with the development and progression of several types of cancer; however, its role in cervical cancer is unclear. Here, we performed a meta-analysis to evaluate the expression levels and clinical significance of HOTAIR in cervical cancer tissue. Relevant literature published by January 2016 was identified in PubMed, EMBase, National Knowledge Infrastructure, Wanfang Database, and Chinese Biomedical Literature Database. We performed statistical analysis, calculated standard mean differences (SMDs), and determined combined odds ratios (ORs) with 95% confidence intervals...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27896589/lipid-nanoparticles-loaded-with-an-antisense-oligonucleotide-gapmer-against-bcl-2-for-treatment-of-lung-cancer
#8
Xinwei Cheng, Qibing Liu, Hong Li, Chen Kang, Yang Liu, Tianqi Guo, Ke Shang, Chengyun Yan, Guang Cheng, Robert J Lee
PURPOSE: Bcl-2 is an anti-apoptotic gene that is frequently overexpressed in human cancers. G3139 is an antisense oligonucleotide against bcl-2 that has shown limited efficacy in clinical trials. Here, we report the synthesis of a new antisense oligonucleotide containing additional chemical modifications and its delivery using nanoparticles. METHODS: An oligonucleotide G3139-GAP was synthesized, which has 2'-O-methyl nucleotides at the 5' and 3' ends based on a "gapmer" design...
November 28, 2016: Pharmaceutical Research
https://www.readbyqxmd.com/read/27895785/expression-and-clinicopathological-significance-of-the-lncrna-hoxa11-as-in-colorectal-cancer
#9
Tong Li, Chengfei Xu, Bin Cai, Meng Zhang, Feng Gao, Jialiang Gan
HOXA11 antisense RNA (HOXA11-AS) is a long non-coding RNA (lncRNA) that is important in determining cancer progression. HOXA11-AS was recently identified as a novel biomarker in lung cancer progression. However, its role in colorectal cancer (CRC) remains poorly understood. The present study aimed to analyze lncRNA HOXA11-AS expression in CRC and investigate a possible association between HOXA11-AS and clinicopathological factors. HOXA11-AS expression was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in 84 CRC tissues and adjacent non-cancerous tissues, in addition to 3 CRC cell lines and 1 human normal colorectal cell line...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27895773/dysregulation-of-long-non-coding-rna-profiles-in-human-colorectal-cancer-and-its-association-with-overall-survival
#10
Lei Yang, Lingling Xu, Qian Wang, Min Wang, Guangyu An
Long non-coding RNAs (lncRNAs) emerged as key regulators of diverse roles during colorectal cancer (CRC) carcinogenesis, but their specific function still remains to be explored. The present study aimed to re-annotate the Affymetrix Human Exon 1.0 ST Array for defining differential lncRNAs in CRC. Their prognostic relevance was also developed for screening key regulators in CRC. The CRC datasets E-GEOD-31737, E-MATB-829, Affymetrix colon cancer dataset and E-GEOD-24550 were re-purposed for searching differential lncRNAs and exploring their association with overall survival (OS)...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27895772/hotair-a-long-non-coding-rna-driver-of-malignancy-whose-expression-is-activated-by-foxc1-negatively-regulates-mirna-1-in-hepatocellular-carcinoma
#11
Dong-Na Su, Shi-Pin Wu, Hong-Tao Chen, Jin-Hua He
Evidence is rapidly accumulating that long non-coding RNAs (lncRNAs) are involved in human tumorigenesis and are dysregulated in multiple cancers, including hepatocellular carcinoma (HCC). lncRNAs can regulate essential pathways that contribute to tumor initiation and progression with tissue specificity, which suggests that lncRNAs may be valuable biomarkers and therapeutic targets. HOX transcript antisense intergenic RNA (HOTAIR) has previously been demonstrated to be an oncogene and a negative prognostic factor in a variety of cancers; however, the factors that contribute to the upregulation of HOTAIR and the interaction between HOTAIR and microRNAs (miRNAs or miRs) are largely unknown...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27895744/identification-of-targets-of-mirna-221-and-mirna-222-in-fulvestrant-resistant-breast-cancer
#12
Pengfei Liu, Manna Sun, Wenhua Jiang, Jinkun Zhao, Chunyong Liang, Huilai Zhang
The present study aimed to identify the differentially expressed genes (DEGs) regulated by microRNA (miRNA)-221 and miRNA-222 that are associated with the resistance of breast cancer to fulvestrant. The GSE19777 transcription profile was downloaded from the Gene Expression Omnibus database, and includes data from three samples of antisense miRNA-221-transfected fulvestrant-resistant MCF7-FR breast cancer cells, three samples of antisense miRNA-222-transfected fulvestrant-resistant MCF7-FR cells and three samples of control inhibitor (green fluorescent protein)-treated fulvestrant-resistant MCF7-FR cells...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27895496/identification-of-lncrna-fam83h-as1-as-a-novel-prognostic-marker-in-luminal-subtype-breast-cancer
#13
Fan Yang, Shi-Xu Lv, Lin Lv, Ye-Huan Liu, Si-Yang Dong, Zhi-Han Yao, Xuan-Xuan Dai, Xiao-Hua Zhang, Ou-Chen Wang
BACKGROUND: Luminal subtype breast cancer accounts for a predominant number of breast cancers. Considering the heterogeneity of the disease, it is urgent to develop novel biomarkers to improve risk stratification and optimize therapy choices. Long non-coding RNA (lncRNA) represents an emerging and understudied class of transcripts that play a significant role in cancer biology. Growing knowledge of cancer-associated lncRNAs contributes to the development of molecular markers for prognosis evaluation and gene therapy...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27895308/epigenetic-inhibition-of-mir-663b-by-long-non-coding-rna-hotair-promotes-pancreatic-cancer-cell-proliferation-via-up-regulation-of-insulin-like-growth-factor-2
#14
Huihua Cai, Yong An, Xuemin Chen, Donglin Sun, Tongbing Chen, Yan Peng, Feng Zhu, Yong Jiang, Xiaozhou He
Pancreatic cancer is one of the most deadly cancers with a poor prognosis. Although microRNAs are involving in the carcinogenesis and development of pancreatic cancer, little information is known regarding the role of miR-663b in pancreatic cancer. In this study, the expression of miR-663b in pancreatic cancer cells was down-regulated by hypermethylation in its putative promoter region, and overexpression of miR-663b repressed cell proliferation, invasion and migration, and induced apoptosis in pancreatic cancer cells...
November 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/27889670/selective-recognition-of-cis-trans-isomers-of-platinum-drugs-and-the-detection-of-triplex-dna-based-on-fluorescence-reversible-model-of-quantum-dots
#15
Xiaoling Xu, Fang Gao, Xincai Xiao, Yan Hu, Chaozhen Zhu, Dan Zhao
The identification of spatial structures of drugs and the researches on their interaction mechanism with DNA are always attractive to the researchers. However, their realization is lack of simple and fast method. This paper reports the establishment of multiple-functional detection platform based on the "turn off-on" model of ZnCdSe quantum dots. In this system, ZnCdSe quantum dots work as the fluorescent probe, platinum anti-cancer drugs as the quencher and triplex DNA as the trapping agent. The seemingly similar cisplatin and transplatin exhibited different fluorescent recovery behaviors due to their difference in structure, and thus realized the selective detection of cisplatin and transplatin with the reaction time set at 10min as well as the quantitation of cisplatin over the range of 2...
November 19, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27879264/differential-regulation-of-the-melanoma-proteome-by-eif4a1-and-eif4e
#16
Cailin E Joyce, Adrienne G Yanez, Akihiro Mori, Akinori Yoda, Johanna S Carroll, Carl D Novina
Small molecules and antisense oligonucleotides that inhibit the translation initiation factors eIF4A1 and eIF4E have been explored as broad-based therapeutic agents for cancer treatment, based on the frequent upregulation of these two subunits of the eIF4F cap-binding complex in many cancer cells. Here we provide support for these therapeutic approaches with mechanistic studies of eIF4F-driven tumor progression in a preclinical model of melanoma. Silencing eIF4A1 or eIF4E decreases melanoma proliferation and invasion...
November 22, 2016: Cancer Research
https://www.readbyqxmd.com/read/27862275/long-noncoding-rna-zfas1-promotes-the-progression-of-colonic-cancer-by-modulating-zeb1-expression
#17
Changyi Fang, Jianbao Zan, Ben Yue, Chenchen Liu, Chenglong He, Dongwang Yan
BACKGROUND AND AIM: Long non-coding RNA ZFAS1 (zinc finger antisense 1) is frequently amplified in hepatocellular carcinoma and promotes metastasis by increasing zinc finger E-box binding homeobox 1 (ZEB1), which can potentiate the progression of epithelial-to-mesenchymal transition (EMT). However, the expression pattern and role of ZFAS1 in colonic cancer remains unknown. The present study aimed to investigate the role of ZFAS1 and its clinical significance in colonic cancer. METHODS: Paired clinical colonic cancer tissue samples and clinicopathologic characteristics of 73 patients were analyzed...
November 11, 2016: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27862063/comparative-expression-analysis-of-hypoxia-inducible-factor-alpha-and-its-natural-occurring-antisense-in-breast-cancer-tissues-and-adjacent-noncancerous-tissues
#18
Behnoosh Tasharrofi, Mohammad Soudyab, Elahe Nikpayam, Mostafa Iranpour, Reza Mirfakhraie, Shaghayegh Sarrafzadeh, Lobat Geranpayeh, Eznollah Azargashb, Arezou Sayad, Soudeh Ghafouri-Fard
Hypoxia-inducible factors (HIFs) have been shown to be upregulated in tumor tissues and linked with tumor progression and metastasis in breast cancer. Among regulatory mechanisms for HIF expression is a natural occurring antisense named aHIF, which has been shown to be overexpressed in breast cancer and influence the level of the HIF-1α transcript. In the present study, we analyzed the expression of HIF-1α and aHIF in breast cancer tissues versus adjacent noncancer tissues (ANCTs) in relation with the clinical and biological behavior of the tumors...
November 12, 2016: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/27855366/hox-antisense-lincrna-hoxa-as2-promotes-tumorigenesis-of-hepatocellular-carcinoma
#19
Fuqiang Wang, Huili Yang, Zhigang Deng, Yongjie Su, QinLiang Fang, Zhenyu Yin
BACKGROUND: Recent studies reveal that long non-coding RNAs (LncRNAs) play critical roles in the proliferation and migration of human cancer. Previous report has shown that LncRNA HOXA-AS2 was involved in carcinoma processes. However, the expression and biological function of HOXA-AS2 in hepatocellular carcinoma (HCC) are poorly understood. METHODS: Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of HOXA-AS2 in HCC tissues and cell lines...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27835600/yap1-enhances-cell-proliferation-migration-and-invasion-of-gastric-cancer-in-vitro-and-in-vivo
#20
Dan Sun, Xiaoting Li, Yingjian He, Wenhui Li, Ying Wang, Huan Wang, Shanshan Jiang, Yan Xin
Yes-associated protein 1 (YAP1) plays an important role in the development of carcinomas such as breast, colorectal, and gastric (GC) cancers, but the role of YAP1 in GC has not been investigated comprehensively. The present study strongly suggests that YAP1 and P62 were significantly up-regulated in GC specimens, compared with normal gastric mucosa. In addition, the YAP1high P62high expression was independently associated with poor prognosis in GC (hazard ratio: 1.334, 95% confidence interval: 1.045-1.704, P = 0...
November 7, 2016: Oncotarget
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