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https://www.readbyqxmd.com/read/29655599/plasma-from-some-cancer-patients-inhibits-adenoviral-ad5f35-vector-transduction-of-dendritic-cells
#1
Ping Jin, Wenjing Chen, Jiaqiang Ren, Steven Chen, Lauren Wood, Yingdong Zhao, Alan Remaley, Chauha Pham, Sheena Lian, Shutong Liu, Hui Liu, Steven Highfill, Jay A Berzofsky, David F Stroncek
BACKGROUND: Pooled AB serum is often used as a media supplement for cell culture but it has the potential to transmit infectious diseases. To avoid this risk, we used autologous plasma as a media supplement for manufacturing dendritic cells (DCs) for cancer immunotherapy. We noticed inconsistencies in the DCs and investigated their nature and cause. METHODS: Adenovirus human epidural growth factor receptor 2 (adHER2/neu) DCs for 21 patients were manufactured from autologous peripheral blood monocytes that were treated with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 for 3 days, transduced with Ad5f35HER2ECTM and then treated with lipopolysaccharide and interferon (IFN)-γ for 1 day...
April 11, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29652619/circulating-cytokine-chemokine-concentrations-respond-to-ionizing-radiation-doses-but-not-radiation-dose-rates-granulocyte-colony-stimulating-factor-and-interleukin-18
#2
Juliann G Kiang, Joan T Smith, Sara R Hegge, Natalia I Ossetrova
Exposure to ionizing radiation is a crucial life-threatening factor in nuclear and radiological incidents. It is known that ionizing radiation affects cytokine/chemokine concentrations in the blood of B6D2F1 mice. It is not clear whether radiation dose rates would vary the physiological response. Therefore, in this study we utilized data from two experiments using B6D2F1 female mice exposed to six different dose rates ranging from low to high rates. In one experiment, mice received a total dose of 8 Gy (LD0/30 ) of 60 Co gamma radiation at four dose rates: 0...
April 13, 2018: Radiation Research
https://www.readbyqxmd.com/read/29652170/pulmonary-transplantation-of-human-ipsc-derived-macrophages-ameliorates-pulmonary-alveolar-proteinosis
#3
Christine Happle, Nico Lachmann, Mania Ackermann, Anja Mirenska, Gudrun Göhring, Kathrin Thomay, Adele Mucci, Miriam Hetzel, Torsten Glomb, Takuji Suzuki, Claudia Chalk, Silke Glage, Oliver Dittrich-Breiholz, Bruce Trapnell, Thomas Moritz, Gesine Hansen
Rationale While the transplantation of induced pluripotent stem cell (iPSC)-derived cells harbors enormous potential for the treatment of pulmonary diseases, in vivo data demonstrating clear therapeutic benefits of human iPSC-derived cells in lung disease models is missing. Objective We here tested the therapeutic potential of iPSC-derived macrophages in a humanized disease model of hereditary pulmonary alveolar proteinosis (herPAP). herPAP is caused by a genetic defect of the GM-CSF-receptor, which leads to disturbed macrophage differentiation and protein/surfactant degradation in the lungs subsequently resulting in severe respiratory insufficiency...
April 13, 2018: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29649688/recombinant-outer-membrane-protein-25c-from-brucella-abortus-induces-th1-and-th2-mediated-protection-against-brucella-abortus-infection-in-mouse-model
#4
Soumya Paul, Bhavani V Peddayelachagiri, Sowmya Nagaraj, Joseph J Kingston, Harsh V Batra
Development of a safe and efficacious vaccine for brucellosis is a long standing challenge for scientists. Recognizing potential antigens towards developing vaccine candidate is crucial. Omp25c, a porin protein of Brucella, is a paralog of two previously identified promising vaccine candidates namely, Omp25 and Omp31, with notable sequence identity. Also, Omp25c is conserved in all major Brucella species. This highlights the possibility of employing this protein in multivalent subunit vaccine based approach of Brucella management...
April 9, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29642398/novel-nanoparticulate-and-ionic-titanium-antigens-for-hypersensitivity-testing
#5
Paul Johan Høl, Einar K Kristoffersen, Nils Roar Gjerdet, Amanda S Pellowe
Titanium is used in a wide variety of materials ranging from medical devices to materials used in everyday life. Adverse biological reactions that could occur in patients, consumers, and workers should be monitored and prevented. There is a lack of available agents to test and predict titanium-related hypersensitivity. The aim of this study was to develop two bioavailable titanium substances in ionic and nanoparticulate form to serve as antigens for hypersensitivity testing in vitro. Peripheral blood mononuclear cells from 20 test subjects were stimulated with the antigens and secretion of monocytic and lymphatic cytokines and chemokines were measured by a multiplex bead assay...
April 6, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29629494/cold-stimuli-facilitate-inflammatory-responses-through-transient-receptor-potential-melastatin-8-trpm8-in-primary-airway-epithelial-cells-of-asthmatic-mice
#6
Haipei Liu, Li Hua, Quanhua Liu, Jun Pan, Yixiao Bao
Bronchial asthma is a chronic inflammatory airway disease that can be aggravated by cold air. However, its mechanism remains largely unknown. As a thermo-sensing cation channel, transient receptor potential melastatin 8 (TRPM8) can be activated by cold stimuli (8-22 °C) and cooling agents. Whereas TRPM8 activation leads to enhanced expression of inflammatory cytokines and mucus hypersecretion in human bronchial epithelial cell lines, no previous study has examined its role in regulating the cold-induced inflammatory responses and its mechanism in asthmatic airway epithelium...
April 9, 2018: Inflammation
https://www.readbyqxmd.com/read/29628925/human-endothelial-cells-modulate-cd4-t-cell-populations-and-enhance-regulatory-t-cell-suppressive-capacity
#7
Wen Chean Lim, Michael Olding, Eugene Healy, Timothy M Millar
Endothelial cells (ECs) line the luminal surface of blood vessels and have an active role in the recruitment of leukocytes, including immune cell activation. Regulatory T cells (Tregs) are immune suppressor cells that maintain peripheral tolerance and must interact with the endothelium as they traffic into tissue. We hypothesized that human ECs could modulate Tregs and their suppressor function. Cocultures of CD4+ T cells with human umbilical vein ECs (HUVECs) or dermal microvascular ECs (HDMECs) were conducted and analyzed for activation and proliferation after 72 and 120 h using flow cytometry...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29625134/il-17-producing-st2-ilc2-plays-a-pathogenic-role-in-lung-inflammation
#8
Ting Cai, Jinxin Qiu, Yan Ji, Wenjing Li, Zhaoyun Ding, Caixia Suo, Jiali Chang, Jingjing Wang, Rui He, Youcun Qian, Xiaohuan Guo, Liang Zhou, Huiming Sheng, Lei Shen, Ju Qiu
BACKGROUND: IL-17 plays a pathogenic role in asthma. ST2- iILC2s (inflammatory group 2 innate lymphoid cells) driven by IL-25 can produce IL-17, while ST2+ nILC2s (natural ILC2s) produce few IL-17. OBJECTIVE: We characterized the ST2+ IL-17+ ILC2s during lung inflammation and determined the pathogenesis and molecular regulation of ST2+ IL-17+ ILC2s. METHODS: Lung inflammation was induced by papain or IL-33. IL-17 production by lung ILC2s from wild-type, Rag1-/- , Rorcgfp/gfp and Ahr-/- mice was examined by flow cytometry...
April 3, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29624724/inflammatory-compound-lipopolysaccharide-promotes-the-survival-of-gm-csf-cultured-dendritic-cell-via-pi3-kinase-dependent-up-regulation-of-bcl-x
#9
Shun Chen, Xinchen Li, Wenjie Zhang, Mengting Zi, Yuekang Xu
As professional antigen-presenting cells, dendritic cells (DCs) initiate and regulate immune responses against inflammation. The invasion of pathogens into the body, however, can in turn cause the change of DCs in both activity and viability, which ultimately affect immune homeostasis. The exact mechanisms that the bacteria utilize to alter the lifespan of DCs, however, are far from clear. In this study, we found that the bacterial wall compound lipopolysaccharide(LPS)can promote the survival of GM-CSF-differentiated DCs (GM-DCs)...
April 6, 2018: Immunology and Cell Biology
https://www.readbyqxmd.com/read/29623491/the-complex-alteration-in-the-network-of-il-17-type-cytokines-in-patients-with-hereditary-angioedema
#10
Francesco Arcoleo, Mariangela Lo Pizzo, Gabriella Misiano, Salvatore Milano, Giuseppina Colonna Romano, Vito Muggeo, Enrico Cillari
Hereditary angioedema (HAE) is a rare autosomic-dominant disorder characterized by a deficiency of C1 esterase inhibitor which causes episodic swellings of subcutaneous tissues, bowel walls and upper airways that are disabling and potentially life-threatening. We evaluated n = 17 patients with confirmed HAE diagnosis during attack and remission state and n = 19 healthy subjects. The samples were tested for a panel of IL (Interleukin)-17-type cytokines (IL-1β, IL-6, IL-10, granulocyte-macrophage colony stimulating factor (GM-CSF), IL-17, IL-21, IL-22, IL-23) and transforming growth factor-beta (TGF-β) subtypes...
April 6, 2018: Clinical and Experimental Medicine
https://www.readbyqxmd.com/read/29622035/ccl17-blockade-as-a-therapy-for-osteoarthritis-pain-and-disease
#11
Ming-Chin Lee, Reem Saleh, Adrian Achuthan, Andrew J Fleetwood, Irmgard Förster, John A Hamilton, Andrew D Cook
BACKGROUND: Granulocyte macrophage-colony stimulating factor (GM-CSF) has been implicated in the pathogenesis of a number of inflammatory diseases and in osteoarthritis (OA). We identified previously a new GM-CSF→Jmjd3→interferon regulatory factor 4 (IRF4)→chemokine (c-c motif) ligand 17 (CCL17) pathway, which is important for the development of inflammatory arthritis pain and disease. Tumour necrosis factor (TNF) can also be linked with this pathway. Here we investigated the involvement of the pathway in OA pain and disease development using the GM-CSF-dependent collagenase-induced OA (CiOA) model...
April 5, 2018: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/29621753/necroptosis-contributes-to-urban-particulate-matter-induced-airway-epithelial-injury
#12
Feng Xu, Man Luo, Lulu He, Yuan Cao, Wen Li, Songmin Ying, Zhihua Chen, Huahao Shen
BACKGROUND/AIMS: Necroptosis, a form of programmed necrosis, is involved in the pathologic process of several kinds of pulmonary diseases. However, the role of necroptosis in particulate matter (PM)-induced pulmonary injury remains unclear. The objective of this study is to investigate the involvement of necroptosis in the pathogenesis of PM-induced toxic effects in pulmonary inflammation and mucus hyperproduction, both in vitro and in vivo. METHODS: PM was administered into human bronchial epithelial (HBE) cells or mouse airways, and the inflammatory response and mucus production were assessed...
March 29, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29618655/il-27-gene-therapy-induces-depletion-of-tregs-and-enhances-the-efficacy-of-cancer-immunotherapy
#13
Jianmin Zhu, Jin-Qing Liu, Min Shi, Xinhua Cheng, Miao Ding, Jianchao C Zhang, Jonathan P Davis, Sanjay Varikuti, Abhay R Satoskar, Lanchun Lu, Xueliang Pan, Pan Zheng, Yang Liu, Xue-Feng Bai
Tumor-induced expansion of Tregs is a significant obstacle to cancer immunotherapy. However, traditional approaches to deplete Tregs are often inefficient, provoking autoimmunity. We show here that administration of IL-27-expressing recombinant adeno-associated virus (AAV-IL-27) significantly inhibits tumor growth and enhances T cell responses in tumors. Strikingly, we found that AAV-IL-27 treatment causes rapid depletion of Tregs in peripheral blood, lymphoid organs, and - most pronouncedly - tumor microenvironment...
April 5, 2018: JCI Insight
https://www.readbyqxmd.com/read/29616857/pilot-trial-of-k562-gm-csf-whole-cell-vaccination-in-mds-patients
#14
Tara M Robinson, Gabrielle T Prince, Chris Thoburn, Erica Warlick, Anna Ferguson, Yvette L Kasamon, Ivan M Borrello, Allan Hess, B Douglas Smith
Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal hematopoietic stem cell malignancies. Currently, approved drugs are given with non-curative intent as the only known cure is allogeneic bone marrow transplantation, which relies on the donor's immune system driving an allogeneic effect. Previous efforts to harness the endogenous immune system have been less successful. We present the results of a pilot study of K562/GM-CSF (GVAX) whole-cell vaccination in MDS patients. The primary objective of safety was met as there were no serious adverse events...
April 4, 2018: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/29616048/tnfsf14-light-exhibits-inflammatory-activities-in-lung-fibroblasts-complementary-to-il-13-and-tgf-%C3%AE
#15
Ricardo da Silva Antunes, Amit K Mehta, Lisa Madge, Joel Tocker, Michael Croft
The cytokine TNFSF14 [homologous to Lymphotoxin, exhibits Inducible expression and competes with HSV Glycoprotein D for binding to HVEM, a receptor expressed on T lymphocytes (LIGHT)] has been shown in mouse models to be important for development of lung tissue remodeling that is characteristic of asthma, idiopathic pulmonary fibrosis (IPF), and systemic sclerosis (SSc). However, its cellular targets are not fully delineated. In the present report, we show that LTβR and HVEM, the receptors for LIGHT, are constitutively expressed in primary human lung fibroblasts (HLFs)...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29611156/practical-application-of-granulocyte-macrophage-colony-stimulating-factor-gm-csf-in-patients-with-wounds
#16
Harold Brem, Raelina Howell, Theresa Criscitelli, Ayelet Senderowicz, Nicolle Siegart, Scott Gorenstein, Brian Gillette
Rapidly evolving advances in wound-care technologies and treatment modalities, including locally injectable granulocyte-macrophage colony-stimulating factor (GM-CSF), are increasingly being used. Based on its role in the stimulation and recruitment of key contributors to wound healing, such as keratinocytes, macrophages, and fibroblasts, GM-CSF is considered to play an essential role in the wound-healing cascade. Synthetic GM-CSF has been shown to have a positive effect on the healing of chronic wounds when given as a local injection in a small number of patients...
March 29, 2018: Surgical Technology International
https://www.readbyqxmd.com/read/29607238/pulmonary-alveolar-proteinosis-in-setting-of-inhaled-toxin-exposure-and-chronic-substance-abuse
#17
Meirui Li, Salem Alowami, Miranda Schell, Clive Davis, Asghar Naqvi
Pulmonary alveolar proteinosis (PAP) is a rare lung disorder in which defects in alveolar macrophage maturation or function lead to the accumulation of proteinaceous surfactant in alveolar space, resulting in impaired gas exchange and hypoxemia. PAP is categorized into three types: hereditary, autoimmune, and secondary. We report a case of secondary PAP in a 47-year-old man, whose risk factors include occupational exposure to inhaled toxins, especially aluminum dust, the use of anabolic steroids, and alcohol abuse, which in mice leads to alveolar macrophage dysfunction through a zinc-dependent mechanism that inhibits granulocyte macrophage-colony stimulating factor (GM-CSF) receptor signalling...
2018: Case Reports in Pulmonology
https://www.readbyqxmd.com/read/29603201/pr-957-mediates-neuroprotection-by-inhibiting-th17-differentiation-and-modulating-cytokine-production-in-a-mouse-model-of-ischemic-stroke
#18
Ying Guo, Xiuju Chen, Daojing Li, Haijie Liu, Yanan Ding, Ranran Han, Yanchao Shi, Xiaofeng Ma
Acute ischemic stroke can induce secondary brain injury by activating an inflammatory response that contributes to clinical impairment. As a specific inhibitor of the immunoproteasome subunit low molecular weight polypeptide 7 (LMP7), PR-957 may participate in regulating pathophysiologic and inflammatory responses in multiple diseases of the CNS. We investigated the neuroprotective properties of PR-957 in a mouse model of stroke, induced by middle cerebral artery occlusion (MCAO). After MCAO and injections of PR-957 or vehicle, we evaluated mice behaviorally using modified Neurological Severity Scores (mNSS) and sensorimotor tests, including the adhesive-removal test, a foot-fault test, and an inclined plane test...
March 30, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29602801/tumour-derived-gm-csf-promotes-granulocyte-immunosuppression-in-mesothelioma-patients
#19
Swati Khanna, Suzanne Graef, Francis Mussai, Anish Thomas, Neha Wali, Bahar Guliz Yenidunya, Constance M Yuan, Betsy Morrow, Jingli Zhang, Firouzeh Korangy, Tim F Greten, Seth M Steinberg, Maryalice Stetler-Stevenson, Gary Middleton, Carmela De Santo, Raffit Hassan
PURPOSE: The cross talk between tumour cells, myeloid cells, and T cells play a critical role in tumour pathogenesis and response to immunotherapies. Although the aetiology of mesothelioma is well understood the impact of mesothelioma on the surrounding immune microenvironment is less well studied. In this study the effect of the mesothelioma microenvironment on circulating and infiltrating granulocytes and T cells is investigated. EXPERIMENTAL DESIGN: Tumour and peripheral blood from mesothelioma patients were evaluated for presence of granulocytes, which were then tested for their T cell suppression...
March 30, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29600428/cbl-mutation-and-mefv-single-nucleotide-variant-are-important-genetic-predictors-of-tumor-reduction-in-glucocorticoid-treated-patients-with-chronic-myelomonocytic-leukemia
#20
Junichi Watanabe, Ken Sato, Yukiko Osawa, Toshikatsu Horiuchi, Shoichiro Kato, Reina Hikota-Saga, Takaaki Maekawa, Takeshi Yamamura, Ayako Kobayashi, Shinichi Kobayashi, Fumihiko Kimura
Glucocorticoid (GC) therapy occasionally relieves tumor-related fever and promotes tumor reduction in patients with chronic myelomonocytic leukemia (CMML). A mutation analysis of 24 patients with CMML revealed the relationship of GC effectiveness, defined as a monocyte reduction of > 50% within 3 days of methylprednisolone administration, with the MEFV single-nucleotide variant (SNV) and CBL mutation. Lipopolysaccharide-stimulated monocytes harboring MEFV E148Q produced greater amounts of IL-1β and TNF-α than did wild-type monocytes; this was effectively suppressed by GC...
March 29, 2018: International Journal of Hematology
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