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sperm antibodies

Ryan Dean Fortune, Raymond J Grill, Christine Beeton, Mark Tanner, Redwan Huq, David S Loose
Spinal cord injury (SCI) results in devastating changes to almost all aspects of a patient's life. In addition to a permanent loss of sensory and motor function, males will also frequently exhibit a profound loss of fertility through poorly understood mechanisms. We demonstrate that SCI causes measureable pathology in the testis both acutely (24 hours) and chronically, up to 1.5 years post injury, leading to loss in sperm motility and viability. SCI has been shown in humans and rats to induce leukocytospermia, with the presence of inflammatory cytokines, anti-sperm antibodies, and reactive oxygen species found within the ejaculate...
October 18, 2016: Journal of Neurotrauma
Yumei Jiang, Dong Cui, Yuefeng Du, Jun Lu, Lin Yang, Jinmei Li, Jing Zhang, Xiaojing Bai
Chronic prostatitis is a risk factor for impaired male fertility potential, and anti-sperm antibodies (ASAs) cause the autoimmune disease immune infertility, which has a negative effect on semen parameters. Current studies have investigated the ASA-positive relationship between chronic prostatitis versus normal controls, but have shown inconsistent results. Hence, we systematic searched the PubMed, EMBASE, Science Direct/Elsevier, Medline, and the Cochrane Library up to October 2015 for case-control studies that involved the ASA-positive relationship between chronic prostatitis patients versus normal controls...
September 30, 2016: Journal of Reproductive Immunology
Beatriz Fernandez-Fuertes, Fernando Narciandi, Cliona O'Farrelly, Alan K Kelly, Sean Fair, Kieran G Meade, Patrick Lonergan
Bovine beta-defensin 126 (BBD126) exhibits preferential expression for the cauda epididymis of males, where it is absorbed onto the tail and post acrosomal region of sperm. The aim of this study was to examine the role of BBD126 in bull sperm function. Fresh and frozen-thawed semen were incubated in the presence of different capacitating agents, as well as with phosphatidylinositol-specific phospholipase C. These treatments, which have been successful in releasing beta-defensin 126 from macaque sperm, proved to be ineffective in bull sperm...
October 5, 2016: Biology of Reproduction
Fernando Narciandi, Beatriz Fernandez-Fuertes, Ilaina Khairulzaman, Hanne Jahns, Deirdre King, Emma K Finlay, Ken H Mok, Sean Fair, Pat Lonergan, Cliona O'Farrelly, Kieran G Meade
β-Defensins are innate immune molecules, often described as antimicrobial peptides (AMPs) because of their bactericidal activity and are now known to have diverse additional functions including in cell signalling, chemoattraction, immunoregulation and reproduction. In humans and primates, β-defensin (BD) 126 has been shown to regulate the ability of sperm to swim through cervical mucus and to protect sperm from attack by the female immune system during transit towards the oviduct. Bovine β-defensin 126 (BBD126) is the ortholog of human defensin 126, and here, computational analysis revealed significant conservation between BBD126 and other mammalian orthologs at the N-terminus, although extensive sequence differences were detected at the C-terminus, implying possible species-specific roles for this β-defensin in reproduction...
October 5, 2016: Biology of Reproduction
Amber R Krauchunas, Andrew Singson
Genetic approaches in C. elegans are complementing the biochemical and antibody based strategies traditionally used to study the molecular underpinnings of fertilization in other organisms. A pair of worm studies, one based on forward genetics and one based on reverse genetics, converge on the sperm immunoglobulin superfamily molecule SPE-45. Loss of spe-45 function leads to the production of sperm that cannot fertilize wild-type eggs. This is a strikingly similar phenotype as those seen in mice lacking the immunoglobulin superfamily protein Izumo1...
2016: Worm
Vidisha Minhas, Abhinav Shrestha, Neerja Wadhwa, Rita Singh, Satish Kumar Gupta
Improvements in long-term female contraception can be achieved by vaccinating with sperm-derived proteins. Here, recombinant proteins comprising either (i) N- (amino acid residues 1-80) or C- (amino acid residues 76-126) terminal fragments of mouse sperm protein 17 (Sp17) fused to the promiscuous T non-B cell epitope of tetanus toxoid (TT), amino acid residues 830-844 followed by di-lysine linker (KK) (TT-KK-Sp17N or TT-KK-Sp17C , respectively) or (ii) mouse equatorin (amino acid residues 21-185) fused to the T non-B cell epitope of bovine RNase (amino acid residues 94-104) were expressed in Escherichia coli...
September 27, 2016: Molecular Reproduction and Development
Natalia Niedźwiecka, Jadwiga Gronczewska, Edward F Skorkowski
Herring spermatozoa exhibit a high activity of NAD-preferring malic enzyme (NAD-ME). This enzyme is involved in the generation of NADH or NADPH in the decarboxylation of malate to form pyruvate and requires some divalent cations to express its activity. In order to confirm that NAD-ME isolated from herring sperm cells is localized in mitochondria, we performed immunofluorescent analysis and assayed spectrophotometrically the malic enzyme reaction. Production of polyclonal rabbit antibodies against NAD-ME from herring spermatozoa enabled identification of mitochondrial localization of this enzyme inside herring spermatozoa...
September 14, 2016: Fish Physiology and Biochemistry
Isabel Barranco, Asta Tvarijonaviciute, Cristina Perez-Patiño, Alejandro Vicente-Carrillo, Inmaculada Parrilla, Jose J Ceron, Emilio A Martinez, Heriberto Rodriguez-Martinez, Jordi Roca
Glutathione peroxidase-5 (GPX5) is an H2O2-scavenging enzyme identified in boar seminal plasma (SP). This study attempted to clarify its origin and role on sperm survival and fertility after artificial insemination (AI). GPX5 was expressed (Western blot and immunocytochemistry using a rabbit primary polyclonal antibody) in testes, epididymis and accessory sex glands (6 boars). SP-GPX5 concentration differed among boars (11 boars, P < 0.001), among ejaculates within boar (44 ejaculates, P < 0.001) and among portions within ejaculate (15 ejaculates)...
2016: PloS One
Romina Russi, María Inés García, Paulina Vignatti, María Florencia Veiga, Mónica Hebe Vazquez-Levin, Carolina Veaute
The immune response has relevant physiological functions both in the male and female reproductive system, and must be tightly controlled to achieve a successful pregnancy. Several immune factors have been related to infertility, among them humoral and cellular immune responses triggered by sperm antigens. The present study was aimed at evaluating the immune profile induced by DNA immunization against the sperm protease proacrosin in CF1 male mice and its effect upon fertility. Immunized animals exhibited higher anti-proacrosin antibodies levels than controls (indirect ELISA), both in serum (p<0...
August 16, 2016: Journal of Reproductive Immunology
Misato Yokoe, Eriko Takayama-Watanabe, Yoko Saito, Megumi Kutsuzawa, Kosuke Fujita, Haruki Ochi, Yuni Nakauchi, Akihiko Watanabe
Internal fertilization ensures successful reproduction of tetrapod vertebrates on land, although how this mode of reproduction evolved is unknown. Here, we identified a novel gene encoding sperm motility-initiating substance (SMIS), a key protein for the internal fertilization of the urodele Cynops pyrrhogaster by Edman degradation of an isolated protein and subsequent reverse transcription polymerase chain reaction. The SMIS gene encoded a 150 amino-acid sequence including the cysteine knot (CK) motif. No gene with substantial similarity to the SMIS was in the data bank of any model organisms...
2016: PloS One
Nicola Bedoni, Lonneke Haer-Wigman, Veronika Vaclavik, Hoai Viet Tran, Pietro Farinelli, Sara Balzano, Beryl Royer-Bertrand, Mohammed E El-Asrag, Olivier Bonny, Christos Ikonomidis, Yan Litzistorf, Konstantinos Nikopoulos, Georgia Yioti, Maria Stefaniotou, Martin McKibbin, Jamie Ellingford, Adam P Booth, Graeme Black, Carmel Toomes, Chris F Inglehearn, Carel B Hoyng, Nathalie Bax, Caroline C W Klaver, Alberta A Thiadens, Fabien Murisier, Daniel F Schorderet, Manir Ali, Frans P M Cremers, Sten Andréasson, Francis L Munier, Carlo Rivolta
Hereditary retinal degenerations encompass a group of genetic diseases characterized by extreme clinical variability. Following next-generation sequencing and autozygome-based screening of patients presenting with a peculiar, recessive form of cone-dominated retinopathy, we identified five homozygous variants [p.(Asp594fs), p.(Gln117*), p.(Met712fs), p.(Ile756Phe), and p.(Glu543Lys)] in the polyglutamylase-encoding gene TTLL5, in eight patients from six families. The two male patients carrying truncating TTLL5 variants also suffered from a substantial reduction in sperm motility and infertility, whereas those carrying missense changes were fertile...
August 22, 2016: Human Molecular Genetics
Won-Hee Song, Young-Joo Yi, Miriam Sutovsky, Stuart Meyers, Peter Sutovsky
Maternal inheritance of mitochondria and mtDNA is a universal principle in human and animal development, guided by selective ubiquitin-dependent degradation of the sperm-borne mitochondria after fertilization. However, it is not clear how the 26S proteasome, the ubiquitin-dependent protease that is only capable of degrading one protein molecule at a time, can dispose of a whole sperm mitochondrial sheath. We hypothesized that the canonical ubiquitin-like autophagy receptors [sequestosome 1 (SQSTM1), microtubule-associated protein 1 light chain 3 (LC3), gamma-aminobutyric acid receptor-associated protein (GABARAP)] and the nontraditional mitophagy pathways involving ubiquitin-proteasome system and the ubiquitin-binding protein dislocase, valosin-containing protein (VCP), may act in concert during mammalian sperm mitophagy...
September 6, 2016: Proceedings of the National Academy of Sciences of the United States of America
Masaki Fukuda, Mitsuhiro Sakase, Moriyuki Fukushima, Hiroshi Harayama
To obtain basic information of bull IZUMO1 (a sperm protein essential for sperm-egg fusion) and disclose possible causes for the impaired fertilizing ability in bull cryopreserved spermatozoa, we investigated this protein in bull spermatozoa collected from various regions of epididymides, freshly ejaculated spermatozoa, acrosome-reacted spermatozoa, and cryopreserved spermatozoa by Western blotting and the triple staining with the anti-IZUMO1 antibody, fluorescein isothiocyanate-peanut agglutinin, and 4',6-diamidino-2-phenylindole...
July 21, 2016: Theriogenology
Randa M Breikaa, Hisham A Mosli, Ashraf B Abdel-Naim
Onabotulinumtoxin A (onabotA) is gaining wide medical use in children. The present study was planned to investigate the influence of its injection on the maturing testicular structures in rats. Immature rats were injected in the bilateral cremaster muscles by onabotA with three doses of (10, 20, and 40 U/kg) three times in a 2-week interval. The effect of these injections on fertility indices was examined. Levels of antisperm antibodies and several apoptosis parameters were also investigated. DNA content in form of ploidy and histopathological alterations were assessed...
August 5, 2016: Journal of Biochemical and Molecular Toxicology
F Lin, C-J Huang, C-S Liu, L-L Guo, G Liu, H-J Liu
This study detected the distribution of laminin during embryonic formation by immunofluorescence. To determine the possible function of laminin on developmental ability of in vitro fertilized embryos, the presumptive zygotes were divided and transferred to CR1aa medium supplemented with different concentrations (0 μg/ml, 5 μg/ml, 10 μg/ml and 20 μg/ml) of laminin. To explore the association with sperm-oocyte fusion, oocytes and/or sperm were pre-incubated with laminin or anti-β1 antibody before insemination...
October 2016: Reproduction in Domestic Animals, Zuchthygiene
Jianbo Chen, Yanli Gu, Zeng Zhang, Wenzhong Zheng, Lihua Yang, Weiren Huang, Shouren Lin, Yuchi Li, Huan Guo, Manling Luo, Qian Ma, Zhimao Jiang, Aifa Tang, Yaoting Gui
Teratozoospermia is generally associated with clinical infertility. Despite numerous studies, the molecular mechanisms underlying male infertility are still poorly understood. In the present study, we demonstrated that deletion of Spata46, a gene encoding a novel protein of unknown function found in mouse testis, was responsible for male subfertility, and the cause of subfertility was characterized as abnormal sperm head shape and a failure of sperm-egg fusion. We also demonstrated that SPATA46 was expressed predominantly in condensed spermatids, with a highly specific localization restricted to the subacrosomal area; the protein is located at the nuclear membrane due to a transmembrane region in the N-terminus of the protein...
September 2016: Biology of Reproduction
T M Hussein, D Elneily, A A Eid, H Abou-ElKhier
Association of hepatitis C virus (HCV) with autoimmune phenomena and impaired semen parameters has been previously reported. The aim of this study was to investigate the influence of HCV infection on the development of antisperm antibodies (ASAs) in HCV-positive males. The study was conducted on 30 HCV-infected individuals and 30 healthy control subjects. In both patients and control groups, liver enzymes and reproductive hormones were measured; computer-assisted semen analysis (CASA) was performed; HCV-RNA in serum was measured and IgG and IgA ASAs in semen were determined...
August 3, 2016: Andrologia
Vladimir V Yushin, Myriam Claeys, Wim Bert
The nematode spermatozoa represent a highly modified (aberrant) type of male gametes that lack a flagellum but for which the process of spermatogenesis culminates in the production of a crawling spermatozoon on the basis of the cytoskeletal component known as "major sperm protein", or MSP. MSP is also known as an important hormone triggering oocyte maturation and ovulation in the model nematode Caenorhabditis elegans, where this protein was first identified. However, direct evidence of MSP localization and of its fate in nematode spermatogenic cells is rare...
October 2016: Micron: the International Research and Review Journal for Microscopy
P Harikrishna, A M Shende, K K Reena, Jobin Thomas, S K Bhure
Regucalcin is a multi-functional protein having roles in calcium homeostasis as well as in anti-apoptotic, anti-prolific and anti-oxidative functions. Recently, it has been reported from the male reproductive tract, but its role in male reproduction needs further investigation; for which the native regucalcin of reproductive origin will be more appropriate. The gel exclusion chromatography followed by diethyl aminoethane cellulose chromatography and two-dimentional cellulose acetate membrane electrophoresis used for its purification are time consuming and less specific...
August 2016: Protein Journal
Yukari Ogura, Yuki Takagishi, Hiroshi Harayama
The aims of this study were to investigate changes in the distribution and molecular mass of boar sperm acrosome-associated 1 (SPACA1) proteins during the acrosome reaction and to discuss validity of SPACA1 proteins as indicators for occurrence of the true acrosome reaction. Boar ejaculated spermatozoa were used for induction of the extracellular Ca(2+)-dependent acrosome reaction (true acrosome reaction) or acrosomal damages (false acrosome reaction) and then subjected to double staining with the anti-SPACA1 protein antibody and FITC-PNA and Western blotting...
September 2016: Animal Reproduction Science
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