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https://www.readbyqxmd.com/read/27895910/proteomic-identification-of-cryostress-in-epididymal-spermatozoa
#1
Sung Jae Yoon, Md Saidur Rahman, Woo Sung Kwon, Do Yeal Ryu, Yoo Jin Park, Myung Geol Pang
BACKGROUND: Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation, thereby reducing sperm quality. We assessed the motility (%), motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining...
2016: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/27890080/antibodies-against-dictyocaulus-viviparus-major-sperm-protein-in-bulk-tank-milk-association-with-clinical-appearance-herd-management-and-milk-production
#2
Johannes Charlier, Aklilu Ghebretinsae, Tom Meyns, Guy Czaplicki, Jozef Vercruysse, Edwin Claerebout
The objective of this study was to conduct a comprehensive field survey using a Dictyocaulus viviparus major sperm protein ELISA on bulk tank milk samples from Belgian dairy herds to gain insights in: (1) the sensitivity (Se) and specificity (Sp) of the test under field conditions; (2) the value of the test to predict a future clinical lungworm outbreak; (3) its associations with milk production parameters and (4) its associations with herd management factors. A total of 1248 herds were sampled, with samplings occurring in the middle ("August") and towards the end ("October") of the grazing season...
December 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27860441/effects-of-human-chorionic-gonadotropin-on-testicular-interstitial-tissues-in-men-with-non-obstructive-azoospermia
#3
S Oka, K Shiraishi, H Matsuyama
Non-obstructive azoospermia is a severe condition because spermatogenesis per se is disrupted. Although microdissection testicular sperm extraction is the standard therapy for non-obstructive azoospermia, sperm retrieval is unsuccessful in approximately 50% of patients. For these patients, we conducted human chorionic gonadotropin-based salvage hormonal therapy, and sperm retrieval was possible in 10-20% of patients. The objectives of this study were to assess the changes in interstitial lesions in patients with non-obstructive azoospermia and to evaluate the effects of human chorionic gonadotropin on these tissues...
November 16, 2016: Andrology
https://www.readbyqxmd.com/read/27857155/fluorescence-and-magnetic-activated-cell-sorting-strategies-to-separate-spermatozoa-involving-plural-contributors-from-biological-mixtures-for-human-identification
#4
Yan Xu, Jianhui Xie, Ronghua Chen, Yu Cao, Yuan Ping, Qingwen Xu, Wei Hu, Dan Wu, Lihua Gu, Huaigu Zhou, Xin Chen, Ziqin Zhao, Jiang Zhong, Rui Li
No effective method has been developed to distinguish sperm cells originating from different men in multi-suspect sexual assault cases. Here we combined MACS and FACS to isolate single donor sperm cells from forensic mixture samples including female vaginal epithelial cells and sperm cells from multiple contributors. Sperms from vaginal swab were isolated by MACS using FITC-conjugated A kinase anchor protein 3 (AKAP3) antibody; target individual sperm cells involving two or three donors were separated by FACS using FITC-labeled blood group A/B antigen antibody...
November 18, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27836756/cyclic-nucleotide-phosphodiesterases-in-human-spermatozoa-and-seminal-fluid-presence-of-an-active-pde10a-in-human-spermatozoa
#5
Loïze Maréchal, Christine Guillemette, Serge Goupil, Patrick Blondin, Pierre Leclerc, François J Richard
BACKGROUND: Cyclic adenosine monophosphate (cAMP) plays a crucial role as a signaling molecule for sperm functions such as capacitation, motility and acrosome reaction. It is well known that cAMP degradation by phosphodiesterase (PDE) enzyme has a major impact on sperm functions. The present study was undertaken to characterize cAMP-PDE activity in human semen. METHODS: cAMP-PDE activity was measured in human sperm and seminal plasma using family specific PDE inhibitors...
November 9, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27821286/immunization-against-lysozyme-like-proteins-affect-sperm-function-and-fertility-in-the-rat
#6
Ganapathy Narmadha, Suresh Yenugu
Proteins of the epididymal and testicular mileu contribute to sperm maturation and a vast majority of them remain uncharacterised. In this study, the role of three Lysozyme-like (LYZL) proteins, namely LYZL1, LYZL4 and LYZL6 in sperm function was assessed using in vitro neutralization and auto antibodies generation model. Rats immunized with LYZL1, LYZL4 and LYZL6 proteins had a litter size of 5.93, 8.47 and 2.10 respectively compared to 9.96 in the control rats. The litter size was further reduced to 4.53, 7...
November 2, 2016: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/27807895/deciphering-the-mechanisms-involving-cenexin-ninein-and-centriolin-in-sperm-maturation-during-epididymal-transit-in-the-domestic-cat
#7
T Rowlison, M A Ottinger, P Comizzoli
The sperm centrosome is an essential organelle with a key role in organizing the sperm aster for proper syngamy and formation of the first mitotic spindle. The sperm cell acquires the functional capability during epididymal transit by incorporation of key factors. The objective of the study was to identify these key maturation proteins, such as ninein and centriolin as well as cenexin-a scaffold protein that serves to bind ninein and centriolin. Epididymal samples were dissected from 17 adult cat testes (>1 year old) and spermatozoa were extracted from the different regions, including rete testis, caput, corpus, cauda and vas deferens...
November 3, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27802063/in-silico-analysis-of-candidate-proteins-sharing-homology-with-streptococcus-agalactiae-proteins-and-their-role-in-male-infertility
#8
Rajeshwari Parida, Luna Samanta
: Leukocytospermia is a physiologic condition defined as human semen with a leukocyte count of >1 x 10(6) cells/ml that is often correlated with male infertility. Moreover, bacteriospermia has been associated with leukocytospermia ultimately leading to male infertility. We have found that semen samples with >1 x 10(6)/ml leukocytes and/or bacteriospermia have oxidative predominance as evidenced by augmented protein carbonyl and lipid peroxidation status of the semen which is implicated in sperm dysfunction...
November 1, 2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/27793655/infectious-burden-and-semen-parameters
#9
E Moretti, N Figura, M S Campagna, F Iacoponi, S Gonnelli, G Collodel
OBJECTIVES: To investigate the relationship between chronic infections detected in serum and semen quality. The pathogen burden is a concept consisting in the observation that, in patients with heart disease, damaging effects of the coronary arteries increase concomitantly with the number of agents responsible of chronic infections to which patients mounted a serological response. Previous observations that H. pylori infection may reduce the semen quality prompted us to perform the present study...
October 25, 2016: Urology
https://www.readbyqxmd.com/read/27771114/ovarian-dysfunction-associated-with-zona-pellucida-based-immunocontraceptive-vaccines
#10
Carolynne J Joonè, Martin L Schulman, Henk J Bertschinger
Despite more than 40 years of research into zona pellucida (ZP)-based vaccines, relatively little is known about their mechanism of action. Early research demonstrated precipitation of ZP glycoproteins by antiovarian antiserum, rendering oocytes resistant to sperm binding in vitro. Subsequent work showed significantly decreased fertilization rates following passive immunization, sparking interest in anti-ZP immunocontraception for human and animal use. The primary mechanism of action of ZP vaccines is generally considered to be an antibody-mediated interference with sperm-oocyte binding and/or fertilization...
September 17, 2016: Theriogenology
https://www.readbyqxmd.com/read/27770032/deficiency-in-outer-dense-fiber-1-is-a-marker-and-potential-driver-of-idiopathic-male-infertility
#11
Louise Hetherington, Elena K Schneider, David DeKretser, Charles H Muller, Hubert Hondermarck, Tony Velkov, Mark A Baker
Globally, ∼1 in 15 men of reproductive age are infertile, yet the precise mechanisms underlying their gamete failure are unknown. Although a semen analysis is performed to determine fertilizing potential, the diagnostic suitability of this analysis has been questioned in several reports, as many men, classified as infertile according to their semen analysis, subsequently turn out to be fertile. Herein, we have used a quantitative (phospho)-proteomic analysis, using enrichment on titanium dioxide followed by ion-trap mass spectrometry (LC-MS/MS), to compare the semen of infertile versus fertile males...
December 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27750479/changes-in-gene-expression-and-metabolism-in-the-testes-of-the-rat-following-spinal-cord-injury
#12
Ryan D Fortune, Raymond J Grill, Christine Beeton, Mark Tanner, Redwan Huq, David S Loose
Spinal cord injury (SCI) results in devastating changes to almost all aspects of a patient's life. In addition to a permanent loss of sensory and motor function, males also will frequently exhibit a profound loss of fertility through poorly understood mechanisms. We demonstrate that SCI causes measureable pathology in the testis both acutely (24 h) and chronically up to 1.5 years post-injury, leading to loss in sperm motility and viability. SCI has been shown in humans and rats to induce leukocytospermia, with the presence of inflammatory cytokines, anti-sperm antibodies, and reactive oxygen species found within the ejaculate...
December 2, 2016: Journal of Neurotrauma
https://www.readbyqxmd.com/read/27743525/association-of-anti-sperm-antibodies-with-chronic-prostatitis-a-systematic-review-and-meta-analysis
#13
Yumei Jiang, Dong Cui, Yuefeng Du, Jun Lu, Lin Yang, Jinmei Li, Jing Zhang, Xiaojing Bai
Chronic prostatitis is a risk factor for impaired male fertility potential, and anti-sperm antibodies (ASAs) cause the autoimmune disease immune infertility, which has a negative effect on semen parameters. Current studies have investigated the ASA-positive relationship between chronic prostatitis versus normal controls, but have shown inconsistent results. Hence, we systematic searched the PubMed, EMBASE, Science Direct/Elsevier, Medline, and the Cochrane Library up to October 2015 for case-control studies that involved the ASA-positive relationship between chronic prostatitis patients versus normal controls...
September 30, 2016: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/27707713/cauda-epididymis-specific-beta-defensin-126-promotes-sperm-motility-but-not-fertilizing-ability-in-cattle
#14
Beatriz Fernandez-Fuertes, Fernando Narciandi, Cliona O'Farrelly, Alan K Kelly, Sean Fair, Kieran G Meade, Patrick Lonergan
Bovine beta-defensin 126 (BBD126) exhibits preferential expression for the cauda epididymis of males, where it is absorbed onto the tail and post acrosomal region of sperm. The aim of this study was to examine the role of BBD126 in bull sperm function. Fresh and frozen-thawed semen were incubated in the presence of different capacitating agents, as well as with phosphatidylinositol-specific phospholipase C. These treatments, which have been successful in releasing beta-defensin 126 from macaque sperm, proved to be ineffective in bull sperm...
October 5, 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27707712/sperm-coating-beta-defensin-126-is-a-dissociation-resistant-dimer-produced-by-epididymal-epithelium-in-the-bovine-reproductive-tract
#15
Fernando Narciandi, Beatriz Fernandez-Fuertes, Ilaina Khairulzaman, Hanne Jahns, Deirdre King, Emma K Finlay, Ken H Mok, Sean Fair, Pat Lonergan, Cliona O'Farrelly, Kieran G Meade
β-Defensins are innate immune molecules, often described as antimicrobial peptides (AMPs) because of their bactericidal activity and are now known to have diverse additional functions including in cell signalling, chemoattraction, immunoregulation and reproduction. In humans and primates, β-defensin (BD) 126 has been shown to regulate the ability of sperm to swim through cervical mucus and to protect sperm from attack by the female immune system during transit towards the oviduct. Bovine β-defensin 126 (BBD126) is the ortholog of human defensin 126, and here, computational analysis revealed significant conservation between BBD126 and other mammalian orthologs at the N-terminus, although extensive sequence differences were detected at the C-terminus, implying possible species-specific roles for this β-defensin in reproduction...
October 5, 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27695649/marriage-shrines-and-worms-impacting-our-understanding-of-mammalian-fertilization
#16
COMMENT
Amber R Krauchunas, Andrew Singson
Genetic approaches in C. elegans are complementing the biochemical and antibody based strategies traditionally used to study the molecular underpinnings of fertilization in other organisms. A pair of worm studies, one based on forward genetics and one based on reverse genetics, converge on the sperm immunoglobulin superfamily molecule SPE-45. Loss of spe-45 function leads to the production of sperm that cannot fertilize wild-type eggs. This is a strikingly similar phenotype as those seen in mice lacking the immunoglobulin superfamily protein Izumo1...
2016: Worm
https://www.readbyqxmd.com/read/27676172/novel-sperm-and-gonadotropin-releasing-hormone-based-recombinant-fusion-protein-achievement-of-100-contraceptive-efficacy-by-co-immunization-of-male-and-female-mice
#17
Vidisha Minhas, Abhinav Shrestha, Neerja Wadhwa, Rita Singh, Satish Kumar Gupta
Improvements in long-term female contraception can be achieved by vaccinating with sperm-derived proteins. Here, recombinant proteins comprising either (i) N- (amino acid residues 1-80) or C- (amino acid residues 76-126) terminal fragments of mouse sperm protein 17 (Sp17) fused to the promiscuous T non-B cell epitope of tetanus toxoid (TT), amino acid residues 830-844 followed by di-lysine linker (KK) (TT-KK-Sp17N or TT-KK-Sp17C , respectively) or (ii) mouse equatorin (amino acid residues 21-185) fused to the T non-B cell epitope of bovine RNase (amino acid residues 94-104) were expressed in Escherichia coli...
September 27, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27630022/nad-preferring-malic-enzyme-localization-regulation-and-its-potential-role-in-herring-clupea-harengus-sperm-cells
#18
Natalia Niedźwiecka, Jadwiga Gronczewska, Edward F Skorkowski
Herring spermatozoa exhibit a high activity of NAD-preferring malic enzyme (NAD-ME). This enzyme is involved in the generation of NADH or NADPH in the decarboxylation of malate to form pyruvate and requires some divalent cations to express its activity. In order to confirm that NAD-ME isolated from herring sperm cells is localized in mitochondria, we performed immunofluorescent analysis and assayed spectrophotometrically the malic enzyme reaction. Production of polyclonal rabbit antibodies against NAD-ME from herring spermatozoa enabled identification of mitochondrial localization of this enzyme inside herring spermatozoa...
September 14, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/27627110/glutathione-peroxidase-5-is-expressed-by-the-entire-pig-male-genital-tract-and-once-in-the-seminal-plasma-contributes-to-sperm-survival-and-in-vivo-fertility
#19
Isabel Barranco, Asta Tvarijonaviciute, Cristina Perez-Patiño, Alejandro Vicente-Carrillo, Inmaculada Parrilla, Jose J Ceron, Emilio A Martinez, Heriberto Rodriguez-Martinez, Jordi Roca
Glutathione peroxidase-5 (GPX5) is an H2O2-scavenging enzyme identified in boar seminal plasma (SP). This study attempted to clarify its origin and role on sperm survival and fertility after artificial insemination (AI). GPX5 was expressed (Western blot and immunocytochemistry using a rabbit primary polyclonal antibody) in testes, epididymis and accessory sex glands (6 boars). SP-GPX5 concentration differed among boars (11 boars, P < 0.001), among ejaculates within boar (44 ejaculates, P < 0.001) and among portions within ejaculate (15 ejaculates)...
2016: PloS One
https://www.readbyqxmd.com/read/27606569/immune-mediators-associated-to-male-infertility-in-a-mouse-model-of-dna-immunization-with-the-sperm-protease-proacrosin
#20
Romina Russi, María Inés García, Paulina Vignatti, María Florencia Veiga, Mónica Hebe Vazquez-Levin, Carolina Veaute
The immune response has relevant physiological functions both in the male and female reproductive system, and must be tightly controlled to achieve a successful pregnancy. Several immune factors have been related to infertility, among them humoral and cellular immune responses triggered by sperm antigens. The present study was aimed at evaluating the immune profile induced by DNA immunization against the sperm protease proacrosin in CF1 male mice and its effect upon fertility. Immunized animals exhibited higher anti-proacrosin antibodies levels than controls (indirect ELISA), both in serum (p<0...
August 16, 2016: Journal of Reproductive Immunology
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