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https://www.readbyqxmd.com/read/28488229/chronic-prostatitis-and-its-detrimental-impact-on-sperm-parameters-a-systematic-review-and-meta-analysis
#1
R A Condorelli, G Ivan Russo, A E Calogero, G Morgia, S La Vignera
PURPOSE: Prostatitis is a very common urogenital disease of the male with prevalence ranging from 2.2 to 9.7% worldwide. Interestingly, some recent evidences have showed a significant association between chronic prostatitis (CP) and male infertility including a detrimental effect on sperm parameters, reduction of zinc concentration on semen sperm and production of anti-semen antibodies (ASAs). The aim of the current meta-analysis was to evaluate the association between CP and alteration of semen parameters...
May 9, 2017: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/28478955/spatial-distribution-of-cannabinoid-receptor-1-and-fatty-acid-amide-hydrolase-in-the-cat-ovary-and-oviduct
#2
Andrea Pirone, Carla Lenzi, Angela Briganti, Francesco Abbate, Maria Levanti, Francesca Abramo, Vincenzo Miragliotta
Involvement of the endocannabinoid system in female reproduction has been extensively described in humans with the cognate receptors and ligands being found in the ovaries and genital tract. In human, an imbalance of the endocannabinoid system is linked with both ectopic pregnancy and infertility. In bovine species anandamide levels regulate aspects of sperm-oviduct interaction. Here we report the immunohistochemical distribution of cannabinoid receptor 1 (CB1R) and fatty acid amide hydrolase (FAAH) in cat ovary and oviduct, using paraffin-embedded tissue samples and commercially available antibodies...
May 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28472863/sexually-selected-size-differences-and-conserved-sexual-monomorphism-of-genital-cortex
#3
Simon M Lauer, Constanze Lenschow, Michael Brecht
The mammalian somatosensory cortex shows marked species-specific differences. How evolution in general and sexual selection in particular shape the somatosensory cortical body representation has not been delineated, however. Here we address this issue by a comparative analysis of genital cortex. Genitals are unique body parts in that they show sexual dimorphism, major changes in puberty and typically more pronounced species differences than other body parts (Hosken and Stockley, 2004). To study the evolution of genital cortex we flattened cortical hemispheres and assembled 104 complete body maps, revealed by cytochrome oxidase activity in layer 4 of 8 rodent and 1 lagomorph species...
May 4, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28442049/prion-protein-2-dublet-gene-prnd-role-in-ovine-semen-capacitation-cryopreservation-and-fertility
#4
L M Ferreira, M Garcia-Herreros, A Domingos, C C Marques, P Mesquita, J P Barbas, M C Baptista, J Pimenta, A E M Horta, J A M Prates, R M L N Pereira
The aim of the present study was to examine the role of Doppel protein in the capacitation process and fertilising ability of both fresh and frozen-thawed (FT) spermatozoa from rams carrying different prion protein 2 (dublet) (PRND) gene polymorphisms. The detection efficacy of new anti-Doppel monoclonal antibodies and PRND mRNA quantification were also explored in ovine spermatozoa. Three different genotypes (AA, GA, GG) were identified for codon 26 of ovine PRND-c.78G>A. Using flow cytometry, a higher fluorescence was detected in fresh compared with FT sperm samples incubated with anti-Doppel primary and fluorescein isothiocyanate-conjugated secondary antibodies (P<0...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28412762/sleep-deprivation-and-late-bedtime-impair-sperm-health-through-increasing-antisperm-antibody-production-a-prospective-study-of-981-healthy-men
#5
Mei-Mei Liu, Li Liu, Liang Chen, Xiao-Jing Yin, Hui Liu, Yan-Hua Zhang, Pei-Ling Li, Shan Wang, Xiao-Xiao Li, Cai-Hong Yu
BACKGROUND The aim of this study was to investigate the effects of sleep duration and bedtime on sperm health, and the possible mechanism involved. MATERIAL AND METHODS We randomly divided 981 healthy Chinese men into groups according to research-set bedtimes (A=8-10 PM, B=after 10 PM, and C=after midnight) and sleep durations: group 1=<6.0 h (short), group 2=7.0-8.0 h (average), and group 3=>9.0 h (long). Sperm morphology, count, survival, and motility were examined according to sleep patterns. Antisperm antibody (ASA) production in semen was determined...
April 16, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28390472/pannexin-channels-increase-propidium-iodide-permeability-in-frozen-thawed-dog-spermatozoa
#6
J L Torres, J Palomino, R D Moreno, M De Los Reyes
Pannexins (Panx) are proteins that form functional single membrane channels, but they have not yet been described in dogs. The aim of the present study was to detect Panx1, Panx2 and Panx3 in frozen-thawed dog spermatozoa using flow cytometry and immunofluorescence analyses, evaluating the relationship of these proteins with propidium iodide (PI) in frozen-thawed spermatozoa. Fresh and frozen-thawed dog spermatozoa from eight dogs were preincubated with 3μM PI with or without 15μM carbenoxolone (CBX) or 1mM probenecid (PBD), two Panx channel inhibitors, and then incubated with rabbit anti-Panx1, anti-Panx2 and anti-Panx3 antibodies (1:200)...
April 10, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28390179/gene-editing-as-applied-to-prevention-of-reproductive-porcine-reproductive-and-respiratory-syndrome
#7
REVIEW
Kristin M Whitworth, Randall S Prather
Porcine Reproductive and Respiratory Syndrome (PRRS) causes severe reproductive failure in sows, as well as transplacental transfer of PRRS virus (PRRSV) to late-gestation fetuses, resulting in abortions, early farrowing, increased number of stillborn piglets, and weak neonatal piglets. PRRSV-infected boars present with anorexia and lethargy, and have decreased sperm quality. The gene for the cellular receptor that the PRRSV uses, Cluster of differentiation 163 (CD163), was edited using Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene-editing technology to create biallelic DNA edits to the CD163 gene in 100% of the offspring...
April 8, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28388234/variable-heavy-chain-domain-derived-from-a-cell-penetrating-anti-dna-monoclonal-antibody-for-the-intracellular-delivery-of-biomolecules
#8
Sun-Woo Im, Pavithra Pravinsagar, Sae-Ran Im, Young-Ju Jang
Targeting and modification of important intracellular proteins using efficient vehicles are invaluable in diagnostic and therapeutic fields. Cell-penetrating antibodies and their fragments can be utilized as vehicles for the delivery of modifiers into cells. In this study, we explored the applicability of variable heavy chain (VH) domain as delivery vehicles for mammalian cells. The characteristics of the recombinant VH domain produced from a cell-penetrating monoclonal anti-double stranded DNA antibody 2C10 were analyzed using flow cytometry, confocal microscopy, cell proliferation assay, and cell cycle analysis in various mammalian cell lines...
April 7, 2017: Immunological Investigations
https://www.readbyqxmd.com/read/28385674/human-derived-fusogenic-peptides-for-the-intracellular-delivery-of-proteins
#9
Kei Sudo, Keisuke Niikura, Kouta Iwaki, Shunshi Kohyama, Kei Fujiwara, Nobuhide Doi
The cytosolic delivery of therapeutic proteins (e.g., antibodies or enzymes) by cell-penetrating peptides (CPPs), such as a human immunodeficiency virus-derived TAT peptide, is facilitated by fusogenic peptides (FPs). For instance, we recently demonstrated that an FP, B18, which is derived from a sea urchin gamete fusion protein, promotes endosomal escape of an enhanced green fluorescent protein (eGFP)-TAT fusion protein directly conjugated to it. However, the potential clinical use of FPs raises concerns because all conventional FPs are non-human-derived...
April 4, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28370610/size-dependent-isolation-of-primordial-germ-cells-from-avian-species
#10
Kyung M Jung, Young M Kim, Tamao Ono, Jae Y Han
Primordial germ cells (PGCs), the precursors of sperm or ova, could be used to generate transgenic animals and interspecies germ-line chimeras, which would facilitate the recovery of endangered species by making their access and manipulation in vitro easier. During early embryogenesis in avian species, PGCs are transported via the bloodstream to the gonadal anlagen. PGCs of most avian species-particularly wild or endangered birds-are not readily isolated from the embryonic bloodstream because germ-cell markers have not yet been defined for them...
March 30, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28349404/analysis-of-chromatin-dynamics-during-drosophila-spermatogenesis
#11
Tim Hundertmark, Ina Theofel, Zeynep Eren-Ghiani, David Miller, Christina Rathke
In the course of spermatogenesis, germ cells undergo dramatic morphological changes that affect almost all cellular components. Therefore, it is impossible to study the process of spermatogenesis in its entirety without detailed morphological analyses. Here, we describe a method to visualize chromatin dynamics in differentiating Drosophila male germ cells using immunofluorescence staining. In addition, we demonstrate how to treat Drosophila sperm before immunofluorescence staining to help reveal epitopes in the highly condensed sperm chromatin that otherwise may be inaccessible to antibodies...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28270212/an-open-label-clinical-trial-to-investigate-the-efficacy-and-safety-of-corifollitropin-alfa-combined-with-hcg-in-adult-men-with-hypogonadotropic-hypogonadism
#12
Eberhard Nieschlag, Pierre-Marc G Bouloux, Barbara J Stegmann, R Ravi Shankar, Yanfen Guan, Anjela Tzontcheva, Christine McCrary Sisk, Hermann M Behre
BACKGROUND: Hypogonadotropic hypogonadism (HH) in men results in insufficient testicular function and deficiencies in testosterone and spermatogenesis. Combinations of human chorionic gonadotropin (hCG) and recombinant follicle-stimulating hormone (recFSH) have been successful in the treatment of HH. Corifollitropin alfa is a long-acting FSH-analog with demonstrated action in women seeking infertility care. The aim of this study was to investigate the efficacy and safety of corifollitropin alfa combined with hCG to increase testicular volume and induce spermatogenesis in men with HH...
March 7, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28244620/testis-specific-isoform-of-angiotensin-converting-enzyme-tace-is-involved-in-the-regulation-of-bovine-sperm-capacitation
#13
Mina Ojaghi, John Kastelic, Jacob Thundathil
We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine testes and sperm; and (iii) determine the role of tACE in capacitation. A 110-kDa protein, consistent with the mass of tACE, was detected in sperm extract by our anti-tACE immunoserum. This immunotarget localized at the acrosomal region and principal piece, but was only expressed in testis of mature bulls...
February 28, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28218625/egress-of-sperm-autoantigen-from-seminiferous-tubules-maintains-systemic-tolerance
#14
Kenneth S K Tung, Jessica Harakal, Hui Qiao, Claudia Rival, Jonathan C H Li, Alberta G A Paul, Karen Wheeler, Patcharin Pramoonjago, Constance M Grafer, Wei Sun, Robert D Sampson, Elissa W P Wong, Prabhakara P Reddi, Umesh S Deshmukh, Daniel M Hardy, Huanghui Tang, C Yan Cheng, Erwin Goldberg
Autoimmune responses to meiotic germ cell antigens (MGCA) that are expressed on sperm and testis occur in human infertility and after vasectomy. Many MGCA are also expressed as cancer/testis antigens (CTA) in human cancers, but the tolerance status of MGCA has not been investigated. MGCA are considered to be uniformly immunogenic and nontolerogenic, and the prevailing view posits that MGCA are sequestered behind the Sertoli cell barrier in seminiferous tubules. Here, we have shown that only some murine MGCA are sequestered...
March 1, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28215872/detection-of-specific-antibodies-in-cats-infected-with-the-lung-nematode-aelurostrongylus-abstrusus
#15
Eva-Maria Zottler, Christina Strube, Manuela Schnyder
Feline aelurostrongylosis, caused by the metastrongylid nematode Aelurostrongylus abstrusus, is an underestimated respiratory parasitosis. Its diagnosis currently mainly relies on the isolation of first stage larvae from fresh faecal samples. The aim of our study was to develop a serological test for the detection of specific antibodies against A. abstrusus by ELISA. We used recombinant major sperm protein (MSP) of the bovine lungworm Dictyocaulus viviparus as detection antigen and evaluated two different ELISA plates (Maxisorp and Immobilizer™ Amino-plate, Nunc Roskilde, Denmark) with two different enzyme systems [alkaline phosphatase (AP) and horseradish peroxidase (HRP)]...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28123480/correlative-study-on-the-jak-stat-psm%C3%AE-3-signal-transduction-pathway-in-asthenozoospermia
#16
Junguo Li, Li Zhang, Bing Li
The aim of the present study was to investigate the possible mechanism of Janus kinase (JAK)-signal transduction and activator of transcription (STAT)/PSMβ3 signaling in the occurrence of asthenozoospermia. We examined seminal fluid samples from 30 cases of asthenozoospermia and 30 healthy controls. Sperm was collected using the Percoll density gradient centrifugation method. The expression of JAK, STAT and PSMβ3 mRNA was assessed by reverse-transcription quantitative PCR and the protein levels of p-JAK, p-STAT and PSMβ3 were measured by western blot analysis...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28111995/the-association-of-different-auto-antibodies-against-ovarian-tissues-and-gonadotropins-and-poor-ovarian-response-in-intracytoplasmic-sperm-injection-cycles
#17
Ladan Mohammadi Yeganeh, Ashraf Moini, Mandana Hemmat, Reza Salman Yazdi, Narges Bagheri Lankarani, Shabnam Khodabakhshi, Arash Behbahanian
This study aimed to assess the possible association between ovarian auto-antibodies and poor ovarian response to controlled ovarian hyperstimulation (COH) in patients undergoing intracytoplasmic sperm injection (ICSI) cycles. In total, 42 poor responders and 43 male factor subjects were enrolled in the study and underwent either a standard long gonadotropin-releasing hormone (GnRH) agonist or antagonist protocol. Anti-ovarian, anti-oocyte, anti-zona pellucida (anti-ZP) and anti-gonadotropin antibodies in their sera and follicular fluid (FF) were measured by an enzyme-linked immunosorbent assay technique (ELISA)...
January 22, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28104696/systematic-characterization-of-human-testis-specific-actin-capping-protein-%C3%AE-3-as-a-possible-biomarker-for-male-infertility
#18
T Soda, Y Miyagawa, N Ueda, K Takezawa, H Okuda, S Fukuhara, K Fujita, H Kiuchi, M Uemura, Y Okamoto, A Tsujimura, H Tanaka, N Nonomura
STUDY QUESTION: Is actin capping protein (CP) β3 involved in human spermatogenesis and male infertility? SUMMARY ANSWER: Human CPβ3 (hCPβ3) is expressed in testis, changes its localization dynamically during spermatogenesis, and has some association with male infertility. WHAT IS KNOWN ALREADY: The testis-specific α subunit of CP (CPα3) was previously identified in human, and mutations in the cpα3 gene in mouse were shown to induce malformation of the sperm head and male infertility...
March 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28062807/junctional-adhesion-molecule-a-expression-in-the-murine-epididymal-tract-and-accessory-organs-and-acquisition-by-maturing-sperm
#19
Kathie Z Wu, Kun Li, Deni S Galileo, Patricia A Martin-DeLeon
STUDY QUESTION: Is junctional adhesion molecule A (JAM-A), a sperm protein essential for normal motility, expressed in the murine post-testicular pathway and involved in sperm maturation? SUMMARY ANSWER: JAM-A is present in the prostate and seminal vesicles and in all three regions of the epididymis where it is secreted in epididymosomes in the luminal fluid and can be delivered to sperm in vitro. WHAT IS KNOWN ALREADY: JAM-A shares with the plasma membrane Ca2+ATPase 4 (PMCA4, the major Ca2+ efflux pump in murine sperm) a common interacting partner, CASK (Ca2+/CaM-dependent serine kinase)...
February 10, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28043371/sperm-bound-antisperm-antibodies-prevent-capacitation-of-bovine-spermatozoa
#20
M S Ferrer, J Klabnik-Bradford, D E Anderson, A C Bullington, R A Palomares, L M J Miller, R Stawicki, M Miesner
It was hypothesized here that sperm-bound antisperm antibodies (ASAs) impair the ability of bovine spermatozoa to undergo capacitation, bind to the zona pellucida, and complete the acrosome reaction. The effect of ASA binding on these functions was evaluated in frozen/thawed spermatozoa from four bulls before and after induction of ASAs. Ejaculates were divided into ASA negative (<10% immunoglobulin [Ig]G- and IgA-bound spermatozoa) or ASA positive (≥20% IgG and/or IgA-bound spermatozoa). The percentage of capacitated (Merocyanine 540 positive) live spermatozoa in response to heparin was lower in ASA-positive than ASA-negative ejaculates (P < 0...
February 2017: Theriogenology
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