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Wenqiang Sun, Jiaxin Li, Jiao Hu, Daxiu Jiang, Chaonan Xing, Tiansong Zhan, Xiufan Liu
Clade of H5N6 subtype Avian Influenza Viruses (AIVs) has become dominant clade in South-East Asia. So far, a total of 16 cases of human infection, including 6 deaths, have been confirmed since 2014. In this study, we systematically investigated the genetic evolution and biological characteristics of these viruses. We first carried out phylogenetic and statistical analysis of all H5N6 viruses that were downloaded from Influenza Research Database, GISAID and isolates from our lab. We found that H5N6 AIVs continued to reassort with other AIVs subtypes since 2014...
June 2018: Veterinary Microbiology
Li-Hsuan Chen, Dong-Hun Lee, Yu-Pin Liu, Wan-Chen Li, David E Swayne, Jen-Chieh Chang, Yen-Ping Chen, Fan Lee, Wen-Jane Tu, Yu-Ju Lin
A highly pathogenic avian influenza A(H5N6) virus of clade was detected in a domestic duck found dead in Taiwan during February 2017. The endemic situation and continued evolution of various reassortant highly pathogenic avian influenza viruses in Taiwan warrant concern about further reassortment and a fifth wave of intercontinental spread.
June 2018: Emerging Infectious Diseases
Rusheng Zhang, Dong Yao, Jingfang Chen, Wen Ye, Xinhua Ou, Tianmu Chen, Biancheng Sun
As of Aug 25, 2017, 17 incidences of human infection and 6 deaths due to the novel H5N6 virus have been reported in China. Genetic analysis of the viral genome revealed that this reassortant virus is highly pathogenic to poultry, and that the virus has a risk of transmission to humans. Accordingly, the development of a rapid, sensitive, and specific molecular diagnostic assay is critical for public health. In this study, a real-time reverse-transcription PCR (RT-PCR) assay was developed to specifically detect the novel H5N6 virus, with primer pairs targeting the hemagglutinin and neuraminidase gene sequences of this virus...
May 2, 2018: Journal of Virological Methods
Masahiro Miyoshi, Rika Komagome, Hiroki Yamaguchi, Setsuko Ishida, Hideki Nagano, Asami Ohnishi, Masayuki Kikuchi, Kazuyo Hamaya, Kohei Shimada, Masataka Fukami, Hajime Shimada, Tsukasa Ohara, Motohiko Okano
No abstract text is available yet for this article.
April 27, 2018: Japanese Journal of Infectious Diseases
Jaeyoung Kim, Jung Hoon Kwon, Jinyoung Jang, Hyojin Lee, Seungki Kim, Young Ki Hahn, Sang Kyung Kim, Kwan Hyi Lee, Seok Lee, Heesoo Pyo, Chang-Seon Song, Joonseok Lee
Rapid and sensitive on-site detection of avian influenza virus (AIV) is the key for achieving near real-time surveillance of AIV and reducing the risk of dissemination. However, unlike the laboratory-prepared transparent buffer solutions containing a single type of influenza virus, distinction between real- and false- positive outputs and detection of low concentrations of AIV in stool specimens or cloacal swabs are difficult. Here, we developed a rapid and background-free lateral flow immunoassay (LFA) platform that utilizes near-infrared (NIR)-to-NIR upconversion nanoparticles (UCNPs) to yield a sensor that detects AIV nucleoproteins (NPs) from clinical samples within 20 min...
April 22, 2018: Biosensors & Bioelectronics
Yu-Pin Liu, Dong-Hun Lee, Li-Hsuan Chen, Yu-Ju Lin, Wan-Chan Li, Shu-Chia Hu, Yen-Ping Chen, David E Swayne, Ming-Shiuh Lee
A H5N6 highly pathogenic avian influenza virus (HPAIV) was detected in a black-faced spoonbill (Platalea minor) found dead in Taiwan during December 2017. Genome sequencing and phylogenetic analyses suggest the hemagglutinin gene belongs to H5 clade Group B. All genes except neuraminidase gene shared high levels of nucleotide identity with H5N8 HPAIV identified from Europe during 2016-2017. Genetically similar H5N6 HPAIV also identified from Japan during November 2017. Enhanced surveillance is required in this region...
April 26, 2018: Infection, Genetics and Evolution
Kate C Mellor, Anne Meyer, Doaa A Elkholly, Guillaume Fournié, Pham T Long, Ken Inui, Pawin Padungtod, Marius Gilbert, Scott H Newman, Timothée Vergne, Dirk U Pfeiffer, Kim B Stevens
Highly pathogenic avian influenza (HPAI) H5N1 virus has been circulating in Vietnam since 2003, whilst outbreaks of HPAI H5N6 virus are more recent, having only been reported since 2014. Although the spatial distribution of H5N1 outbreaks and risk factors for virus occurrence has been extensively studied, there have been no comparative studies for H5N6. Data collected through active surveillance of Vietnamese live bird markets (LBMs) between 2011 and 2015 were used to explore and compare the spatiotemporal distributions of H5N1- and H5N6-positive LBMs...
2018: Frontiers in Veterinary Science
J-Y Noh, D-H Lee, S-S Yuk, J-H Kwon, E-O Tseren-Ochir, W-T Hong, J-H Jeong, S Jeong, C-S Song
The pathogenicity and transmissibility of a reassortant clade avian influenza A (H5N6) virus were evaluated in ferrets. Virus excretion was detected in the upper respiratory tract, but the ferrets did not show any clinical signs of infection. Transmission did not occur between cohoused or respiratory droplet-contact ferrets.
April 19, 2018: Transboundary and Emerging Diseases
Ruyi Gao, Min Gu, Kaituo Liu, Qunhui Li, Juan Li, Liwei Shi, Xiuli Li, Xiaoquan Wang, Jiao Hu, Xiaowen Liu, Shunlin Hu, Sujuan Chen, Daxin Peng, Xinan Jiao, Xiufan Liu
Most clade H5NX subtype avian influenza viruses possess a T160A amino acid substitution in the hemagglutinin (HA) protein that has been shown to affect the receptor binding properties of a clade 2.3.4 H5N1 virus. However, the effect of this single site mutation on the HA backbone of clade H5NX viruses remains unclear. In this study, two H5N6 field isolates possessing HA-160A with dual α-2,3 and α-2,6 receptor binding properties (Y6 virus) and HA-160T with α-2,3 receptor binding affinity (HX virus), respectively, were selected to generate HA mutants containing all of the internal genes from A/PR8/H1N1 virus for comparative investigation...
April 2018: Veterinary Microbiology
Xiuming Peng, Fumin Liu, Haibo Wu, Xiaorong Peng, Yufan Xu, Liyan Wang, Bin Chen, Tao Sun, Fan Yang, Shujing Ji, Nanping Wu
H5N6 avian influenza viruses (AIVs) can cause severe pneumonia and death in humans. However, the molecular determinants of H5N6 influenza virus mammalian adaption are still unclear. Three amino acid substitutions (HA A150V, PA A343T, PB2 E627K) are observed in H5N6 virus A/duck/Zhejiang/6D2/2013 (6D2) in lung-to-lung passage in mice. These substitutions are crucial to the pathogenicity of mouse-adapted virus. In this study, we investigated the contribution of each amino acid substitution in the virus by reverse genetics...
2018: Frontiers in Microbiology
Hyeok-Il Kwon, Eun-Ha Kim, Young-Il Kim, Su-Jin Park, Young-Jae Si, In-Won Lee, Hiep Dinh Nguyen, Kwang Min Yu, Min-Ah Yu, Ju Hwan Jung, Won-Suk Choi, Jin Jung Kwon, Su Jeong Ahn, Yun Hee Baek, Dam Van Lai, Ok-Jun Lee, Si-Wook Kim, Min-Suk Song, Sun-Woo Yoon, Chul-Joong Kim, Richard J Webby, In-Pil Mo, Young Ki Choi
Highly pathogenic avian influenza (HPAI) A(H5N6) and A(H5N8) virus infections resulted in the culling of more than 37 million poultry in the Republic of Korea during the 2016/17 winter season. Here we characterize two representative viruses, A/Environment/Korea/W541/2016 [Em/W541(H5N6)] and A/Common Teal/Korea/W555/2017 [CT/W555(H5N8)], and evaluate their zoonotic potential in various animal models. Both Em/W541(H5N6) and CT /W555(H5N8) are novel reassortants derived from various gene pools of wild bird viruses present in migratory waterfowl arising from eastern China...
March 14, 2018: Emerging Microbes & Infections
Haixin Ai, Xuewei Wu, Mengyuan Qi, Li Zhang, Huan Hu, Qi Zhao, Jian Zhao, Hongsheng Liu
In recent years, new strains of influenza virus such as H7N9, H10N8, H5N6 and H5N8 had continued to emerge. There was an urgent need for discovery of new anti-influenza virus drugs as well as accurate and efficient large-scale inhibitor screening methods. In this study, we focused on six influenza virus proteins that could be anti-influenza drug targets, including neuraminidase (NA), hemagglutinin (HA), matrix protein 1 (M1), M2 proton channel (M2), nucleoprotein (NP) and non-structural protein 1 (NS1). Structure-based molecular docking was utilized to identify potential inhibitors for these drug targets from 13144 compounds in the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform...
June 2018: Interdisciplinary Sciences, Computational Life Sciences
Young-Il Kim, Young-Jae Si, Hyeok-Il Kwon, Eun-Ha Kim, Su-Jin Park, Norbert John Robles, Hiep Dinh Nguyen, Min-Ah Yu, Kwang-Min Yu, Youn-Jeong Lee, Myoung-Heon Lee, Young Ki Choi
We investigated influenza A(H5N6) viruses from migratory birds in Chungnam and Gyeonggi Provinces, South Korea following a reported die-off of poultry in nearby provinces in November 2017. Genetic analysis and virulence studies in chickens and ducks identified our isolate from December 2017 as a novel highly pathogenic avian influenza virus. It resulted from reassortment between the highly virulent H5N8 strain from Korea with the N6 gene from a low-pathogenic H3N6 virus from the Netherlands.
February 2018: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
Kaijian Luo, Kai Zhang, Lu Liu, Xuejuan Shen, Peirong Jiao, Yafen Song, Jiamin Lv, Mei Wang, Yongfa Liu, Wenbao Qi, Tao Ren, David M Irwin, Ming Liao, Yongyi Shen
The H5N6 highly pathogenic avian influenza viruses (HPAIVs) have circulated within poultry in China since 2013. Infections of H5N6 in wild birds were reported since 2014. In order to investigate the infection history of H5N6 in wild birds, we conducted a retrospective analysis of H5 positive wild bird samples collected in 2013, the year H5N6 was discovered in poultry. We isolated a new HPAI H5N6 virus from a dead heron collected in 2013. The virus had high identity in all eight gene sequences to those collected from poultry in 2013 (for example, A/chicken/Shenzhen/1845/2013, 99...
April 2018: Infection, Genetics and Evolution
Han Ju, Jian Zhang, Zhuosen Sun, Zheng Huang, Wenbao Qi, Bing Huang, Peng Zhan, Xinyong Liu
Inspired by our initial discovery about a series of neuraminidase (NA) inhibitors targeting the 150-cavity, in present study, we designed, synthesized, and biologically tested a panel of novel oseltamivir derivatives with C-1 modification, targeting the 430-cavity, an additional binding site which widely and stably existed in both group-1 and group-2 NAs. Some of the synthesized compounds displayed robust anti-influenza potencies against H5N1 and H5N6 viruses. Among them, compound 8b exerted the greatest inhibition, with IC50 values of 0...
February 25, 2018: European Journal of Medicinal Chemistry
Hien Dang Thanh, Van Trung Tran, Duc Tan Nguyen, Vu-Khac Hung, Wonyong Kim
Avian influenza A H5N6 virus is a highly contagious infectious agent that affects domestic poultry and humans in South Asian countries. Vietnam may be an evolutionary hotspot for influenza viruses and therefore could serve as a source of pandemic strains. In 2015, two novel reassortant H5N6 influenza viruses designated as A/quail/Vietnam/CVVI01/2015 and A/quail/Vietnam/CVVI03/2015 were isolated from dead quails during avian influenza outbreaks in central Vietnam, and the whole genome sequences were analyzed...
February 2018: Comparative Immunology, Microbiology and Infectious Diseases
Ryota Tsunekuni, Yuji Yaguchi, Yuki Kashima, Kaoru Yamashita, Nobuhiro Takemae, Junki Mine, Taichiro Tanikawa, Yuko Uchida, Takehiko Saito
From 29 November 2016 to 24 January 2017, sixty-three cases of H5N6 highly pathogenic avian influenza virus (HPAIV) infections were detected in wild birds in Ibaraki Prefecture, Japan. Here, we analyzed the genetic, temporal, and geographic correlations of these 63 HPAIVs to elucidate their dissemination throughout the prefecture. Full-genome sequence analysis of the Ibaraki isolates showed that 7 segments (PB2, PB1, PA, HA, NP, NA, NS) were derived from G1.1.9 strains while the M segment was from G1.1 strains; both groups of strains circulated in south China...
May 2018: Archives of Virology
Nancy Beerens, Guus Koch, Rene Heutink, Frank Harders, D P Edwin Vries, Cynthia Ho, Alex Bossers, Armin Elbers
A novel highly pathogenic avian influenza A(H5N6) virus affecting wild birds and commercial poultry was detected in the Netherlands in December 2017. Phylogenetic analysis demonstrated that the virus is a reassortant of H5N8 clade viruses and not related to the Asian H5N6 viruses that caused human infections.
April 17, 2018: Emerging Infectious Diseases
Shaoxia Lu, Zongzheng Zhao, Jiajie Zhang, Weidong Wang, Xin He, Mengqi Yu, Chunmao Zhang, Xiang Li, Zhendong Guo, Xiaoyu Yang, Lina Liu, Min Zhi, Tian Fu, Xinru Lv, Wenge Ma, Mengying Liao, Hongliang Chai, Linna Liu, Jun Qian, Jianzhang Ma
No abstract text is available yet for this article.
January 24, 2018: Emerging Microbes & Infections
Kaituo Liu, Min Gu, Shunlin Hu, Ruyi Gao, Juan Li, Liwei Shi, Wenqi Sun, Dong Liu, Zhao Gao, Xiulong Xu, Jiao Hu, Xiaoquan Wang, Xiaowen Liu, Sujuan Chen, Daxin Peng, Xinan Jiao, Xiufan Liu
During surveillance for avian influenza viruses, three H5N6 viruses were isolated in chickens obtained from live bird markets in eastern China, between January 2015 and April 2016. Sequence analysis revealed a high genomic homology between these poultry isolates and recent human H5N6 variants whose internal genes were derived from genotype S H9N2 avian influenza viruses. Glycan binding assays revealed that all avian H5N6 viruses were capable of binding to both human-type SAα-2,6Gal receptors and avian-type SAα-2,3Gal receptors...
April 2018: Archives of Virology
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