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https://www.readbyqxmd.com/read/29232147/-incorporation-of-mrna-in-lamellar-lipid-matrices-for-parenteral-administration
#1
Antje Ziller, Sara S Nogueira, Eva Huehn, Sergio S Funari, Gerald Brezesinski, Hermann Hartmann, Ugur Sahin, Heinrich Haas, Peter Langguth
Insertion of high molecular weight messenger RNA (mRNA) into lyotropic lipid phases as model systems for controlled release formulations for the mRNA was investigated. Low fractions of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were used as an anchor to load the mRNA into a lamellar lipid matrix. Dispersions of zwitterionic lipid in the aqueous phase in the presence of increasing fractions of mRNA and cationic lipid were prepared, and the molecular organization was investigated as a function of mRNA and cationic lipid fraction...
December 12, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29229905/single-cell-rna-sequencing-uncovers-transcriptional-states-and-fate-decisions-in-haematopoiesis
#2
Emmanouil I Athanasiadis, Jan G Botthof, Helena Andres, Lauren Ferreira, Pietro Lio, Ana Cvejic
The success of marker-based approaches for dissecting haematopoiesis in mouse and human is reliant on the presence of well-defined cell surface markers specific for diverse progenitor populations. An inherent problem with this approach is that the presence of specific cell surface markers does not directly reflect the transcriptional state of a cell. Here, we used a marker-free approach to computationally reconstruct the blood lineage tree in zebrafish and order cells along their differentiation trajectory, based on their global transcriptional differences...
December 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/29228426/lncrna-hotair-contributes-to-the-tumorigenesis-of-nasopharyngeal-carcinoma-via-up-regulating-fasn
#3
D-D Ma, L-L Yuan, L-Q Lin
OBJECTIVE: The therapeutic options for nasopharyngeal carcinoma (NPC) treatment have been restricted mainly to radiotherapy or chemotherapy, and the clinical treatment effect remains unsatisfactory. The primary purpose of this study was to investigate the molecular mechanisms of NPC and to find effective novel therapeutic targets for NPC. PATIENTS AND METHODS: In order to analyze the expression level of lncRNA HOTAIR and FASN in human NPC clinical tissues or NPC cells, total RNA was extracted with TRIzol and the relative mRNA expression levels were detected by quantitative Real-time PCR...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29226652/a-pooled-analysis-of-the-clinical-utilities-of-long-non-coding-rna-based-molecular-signature-for-diffuse-large-b-cell-lymphoma
#4
Pei Xu, Xiaoli Chen, Panke Su
BACKGROUND: Long non-coding RNAs (lncRNAs) have been highlighted as promising biomarkers for several types of malignancies. The current meta-analysis sought to investigate the clinical value of lncRNA-based molecular signatures as biomarkers for diagnosis, subtype classification, and prognosis in diffuse large B cell lymphoma (DLBCL). METHODS: A systematic search of the online databases was carried out and data were retrieved by pairs of independent reviewers. Effect sizes of the diagnostic parameters were combined using a quantitative meta-analysis...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29223394/islet-%C3%AE-cell-an-endocrine-cell-secreting-mirnas
#5
Aisen Zhang, Dameng Li, Yucheng Liu, Jing Li, Yujing Zhang, Chen-Yu Zhang
The pancreas is an important endocrine organ. Pancreatic beta (β) cells secrete insulin to regulate the metabolism of glucose and maintain the stability of blood glucose. MicroRNAs (miRNAs), small (20-25 nt) non-coding RNA molecules, function in target gene silencing through post-transcriptional patterns, and multiple miRNAs regulate insulin secretion, insulin signaling pathways in pancreatic β cells and islet activity. In recent years, it has been found that many types of cells can secrete regulatory miRNA, but whether islet cells can secrete miRNA has not been clarified yet...
December 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29221147/long-noncoding-rna-linc00673-epigenetically-suppresses-klf4-by-interacting-with-ezh2-and-dnmt1-in-gastric-cancer
#6
Ming-Chen Ba, Hui Long, Shu-Zhong Cui, Yuan-Feng Gong, Zhao-Fei Yan, Yin-Bing Wu, Yi-Nuo Tu
Long non-coding RNAs (lncRNAs), a variety of transcripts without protein coding ability, have recently been reported to play vital roles in gastric cancer (GC) development and progression. However, the biological role of long non-coding RNA LINC00673 in GC is not fully known. In the study, we found that LINC00673 expression was dramatically higher in gastric cancer tissues compared with adjacent normal tissues, and positively associated with lymph node metastasis, distant metastasis and TNM stage in patients...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29220646/dissecting-cell-type-composition-and-activity-dependent-transcriptional-state-in-mammalian-brains-by-massively-parallel-single-nucleus-rna-seq
#7
Peng Hu, Emily Fabyanic, Deborah Y Kwon, Sheng Tang, Zhaolan Zhou, Hao Wu
Massively parallel single-cell RNA sequencing can precisely resolve cellular diversity in a high-throughput manner at low cost, but unbiased isolation of intact single cells from complex tissues such as adult mammalian brains is challenging. Here, we integrate sucrose-gradient-assisted purification of nuclei with droplet microfluidics to develop a highly scalable single-nucleus RNA-seq approach (sNucDrop-seq), which is free of enzymatic dissociation and nucleus sorting. By profiling ∼18,000 nuclei isolated from cortical tissues of adult mice, we demonstrate that sNucDrop-seq not only accurately reveals neuronal and non-neuronal subtype composition with high sensitivity but also enables in-depth analysis of transient transcriptional states driven by neuronal activity, at single-cell resolution, in vivo...
December 7, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29217771/rad52-is-required-for-rna-templated-recombination-repair-in-post-mitotic-neurons
#8
Starr Welty, Yaqun Teng, Zhuobin Liang, Weixing Zhao, Laurie Sanders, J Timothy Greenamyre, Maria Eulalia Rubio, Amantha Thathiah, Ravindra Kodali, Ronald Wetzel, Arthur S Levine, Li Lan
It has been long assumed that post-mitotic neurons only utilize the error-prone non-homologous end joining (NHEJ) pathway to repair double strand breaks (DSBs) associated with oxidative damage to DNA, given the inability of non-replicating neuronal DNA to utilize a sister chromatid template in the less error-prone homologous recombination (HR) repair pathway. However, we and others recently have found that active transcription triggers a replication-independent recombinational repair mechanism in the G0/G1 phase of the cell cycle...
December 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29217667/critical-contribution-of-na-ca2-exchanger-to-the-ca2-mediated-vasodilation-activated-in-endothelial-cells-of-resistance-arteries
#9
Mauricio A Lillo, Pablo S Gaete, Mariela Puebla, Nicolás M Ardiles, Inés Poblete, Alvaro Becerra, Felipe Simon, Xavier F Figueroa
Na+-Ca2+ exchanger (NCX) contributes to control the intracellular free Ca2+ concentration ([Ca2+]i), but the functional activation of NCX reverse mode (NCXrm) in endothelial cells is controversial. We evaluated the participation of NCXrm-mediated Ca2+ uptake in the endothelium-dependent vasodilation of rat isolated mesenteric arterial beds. In phenylephrine-contracted mesenteries, the acetylcholine (ACh)-induced vasodilation was abolished by treatment with the NCXrm blockers SEA0400, KB-R7943, or SN-6. Consistent with that, the ACh-induced hyperpolarization observed in primary cultures of mesenteric endothelial cells and in smooth muscle of isolated mesenteric resistance arteries was attenuated by KB-R7943 and SEA0400, respectively...
December 7, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29217453/tumor-suppressing-effect-of-silencing-of-annexin-a3-expression-in-breast-cancer
#10
Ju-Yeon Kim, Eun Jung Jung, Hee Jin Park, Jeong-Hee Lee, Eun Jin Song, Seung-Jin Kwag, Ji-Ho Park, Taejin Park, Sang-Ho Jeong, Chi-Young Jeong, Young-Tae Ju, Young-Joon Lee, Soon-Chan Hong
INTRODUCTION: Annexin A3 (ANXA3) participates in various tumor-associated biological processes, including tumor initiation, progression, and metastasis. The present study was designed to investigate the expression and function of ANXA3 in breast cancer cells. MATERIALS AND METHODS: Annexin A3 protein expression in breast cancer cell lines was evaluated using Western blot analysis. ANXA3 expression in MDA-MB 231 breast cancer cells was silenced by RNA interference, and the effects of RNA silencing on cell proliferation, colony forming ability, wound-healing, and invasiveness were evaluated...
November 21, 2017: Clinical Breast Cancer
https://www.readbyqxmd.com/read/29217199/efficient-generation-of-transgene-and-feeder-free-induced-pluripotent-stem-cells-from-human-dental-mesenchymal-stem-cells-and-their-chemically-defined-differentiation-into-cardiomyocytes
#11
Xiaobing Tan, Qingli Dai, Tao Guo, Jingshu Xu, Qingyuan Dai
Advance in stem cell research resulted in several processes to generate induced pluripotent stem cells (iPSCs) from adult somatic cells. In our previous study, the reprogramming of iPSCs from human dental mesenchymal stem cells (MSCs) including SCAP and DPSCs, has been reported. Herein, safe iPSCs were reprogrammed from SCAP and DPSCs using non-integrating RNA virus vector, which is an RNA virus carrying no risk of altering host genome. DPSCs- and SCAP-derived iPSCs exhibited the characteristics of the classical morphology with human embryonic stem cells (hESCs) without integration of foreign genes, indicating the potential of their clinical application...
December 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29216356/improved-egfr-mutation-detection-using-combined-exosomal-rna-and-circulating-tumor-dna-in-nsclc-patient-plasma
#12
A K Krug, D Enderle, C Karlovich, T Priewasser, S Bentink, A Spiel, K Brinkmann, J Emenegger, D G Grimm, E Castellanos-Rizaldos, J W Goldman, L V Sequist, J-C Soria, D R Camidge, S M Gadgeel, H A Wakelee, M Raponi, M Noerholm, J Skog
Background: A major limitation of circulating tumor DNA (ctDNA) for somatic mutation detection has been the low level of ctDNA found in a subset of cancer patients. We investigated whether using a combined isolation of exosomal RNA (exoRNA) and cell-free DNA (cfDNA) could improve blood-based liquid biopsy for EGFR mutation detection in NSCLC patients. Patients and methods: Matched pretreatment tumor and plasma were collected from 84 patients enrolled in TIGER-X (NCT01526928), a Ph1/2 study of rociletinib in mutant EGFR NSCLC patients...
December 5, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/29214886/clinical-and-molecular-aspects-of-lead-toxicity-an-update
#13
Prasenjit Mitra, Shailja Sharma, Purvi Purohit, Praveen Sharma
Lead toxicity is a major public health issue in developed and developing countries. Both acute and chronic lead exposure has the potential to cause many deleterious systematic effects including hypertension, frank anemia, cognitive deficits, infertility, immune imbalances, delayed skeletal and deciduous dental development, vitamin D deficiency, and gastrointestinal effects. The underlying mechanisms for all these systemic effects have not been elucidated completely. However, the most plausible cause is free radical damage...
December 7, 2017: Critical Reviews in Clinical Laboratory Sciences
https://www.readbyqxmd.com/read/29214776/14-3-3%C3%AE-overexpression-is-associated-with-poor-prognosis-in-ovarian-cancer
#14
Hyun Jung Kim, Sun Hee Sung, Chan Young Kim, Moon Kyoung Bae, Min Sun Cho, Yun Hwan Kim, Seung Cheol Kim, Woong Ju
PURPOSE: 14-3-3ζregulates cell signaling, cell cycle progression, and apoptosis, and its overexpression is associated with disease recurrence and poor clinical outcomes in some solid tumors. However, its clinicopathological role in ovarian cancer is unknown. Our goal was to investigate whether 14-3-3ζis associated with ovarian cancer prognosis. MATERIALS AND METHODS: We examined 14-3-3ζexpression by immunohistochemistry in ovarian cancer tissues obtained from 88 ovarian cancer patients...
January 2018: Yonsei Medical Journal
https://www.readbyqxmd.com/read/29214477/prevalence-of-occult-hepatitis-c-virus-among-hemodialysis-patients-in-tanta-university-hospitals-a-single-center-study
#15
Ghada Abdelmoemen, Samy Abdelkader Khodeir, Sabry Abou-Saif, Abdelrahman Kobtan, Sherief Abd-Elsalam
Occult hepatitis C virus infection (OCI) is a newly defined type of infection by the chronic hepatitis virus (HCV) distinguished by the existence of HCV RNA in liver tissue and/or peripheral blood mononuclear cells (PBMCs) in patients whose plasma are devoid of both positive serology and RNA. Patients on maintenance hemodialysis evince a higher HCV prevalence than the general population due to high nosocomial transmission by the dialysis units. We investigated the prevalence of occult HCV infection in patients attending our university hemodialysis centers for maintenance hemodialysis...
December 6, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29212943/the-cellular-chaperone-heat-shock-protein-90-is-required-for-foot-and-mouth-disease-virus-capsid-precursor-processing-and-assembly-of-capsid-pentamers
#16
Joseph Newman, Amin S Asfor, Stephen Berryman, Terry Jackson, Stephen Curry, Tobias J Tuthill
Productive picornavirus infection requires the hijack of host cell pathways to aid with the different stages of virus entry, synthesis of the viral polyprotein and viral genome replication. Many picornaviruses, including foot-and-mouth disease virus (FMDV), assemble capsids via the multimerisation of several copies of a single capsid precursor protein into a pentameric subunit which further encapsidates the RNA. Pentamer formation is preceded by co- and post-translational modification of the capsid precursor (P1-2A) by viral and cellular enzymes, and the subsequent rearrangement of P1-2A into a structure amenable to pentamer formation...
December 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29212414/a-suite-of-dna-methylation-markers-that-can-detect-most-common-human-cancers
#17
Lukas Vrba, Bernard W Futscher
Cancer-specific DNA methylation from the tumor derived fraction of cell free DNA found in blood samples could be used for minimally invasive detection and monitoring of cancer. The knowledge of marker regions with cancer-specific DNA methylation is necessary to the success of such a process. We analyzed the largest cancer DNA methylation dataset available-TCGA Illumina HumanMethylation450 data with over 8,500 tumors-in order to find cancer-specific DNA methylation markers for most common human cancers. First, we identified differentially methylated regions for individual cancer types and those were further filtered against data from normal tissues to obtain marker regions with cancer-specific methylation, resulting in a total of 1,250 hypermethylated and 584 hypomethylated marker CpGs...
December 7, 2017: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/29210662/response-to-doxorubicin-of-exfoliated-human-buccal-epithelium-cells-comparison-of-three-methods-of-cell-staining-and-calcium-assessment
#18
Yuriy Shckorbatov, Dmitriy Miroshnik, Igor Kovalenko
BACKGROUND: Doxorubicin is an anthracycline antibiotic which inhibits DNA and RNA synthesis intercalating DNA double helix and inducing free radicals. Doxorubicin is used in treatment of cancer diseases. The influence of Doxorubicin on human buccal cell was used as a model for assessment of toxic effects in vitro. OBJECTIVE: We studied the possibility of using the process of heterochromatinization in cell nuclei in toxicological investigations of drugs in vitro...
December 6, 2017: Current Drug Discovery Technologies
https://www.readbyqxmd.com/read/29207012/microrna%C3%A2-30a%C3%A2-5p-suppresses-tumor-cell-proliferation-of-human-renal-cancer-via-the-mtdh-pten-akt-pathway
#19
Jianmin Li, Changying Li, Hongjie Li, Ting Zhang, Xiaodong Hao, Jiwu Chang, Yong Xu
The present study aimed to explore the effects of microRNA (miRNA)‑30a‑5p on tumor proliferation and to seek a potential therapeutic target for the treatment of human renal cancer. The results demonstrated that the expression levels of miRNA‑30a‑5p were reduced in tumor samples from patients with renal cancer compared with in normal tissue samples. Overall survival and disease‑free survival were increased in patients with renal cancer and high miRNA‑30a‑5p expression compared with in those with low miRNA‑30a‑5p...
November 17, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29202695/single-cell-rna-seq-analysis-reveals-dynamic-trajectories-during-mouse-liver-development
#20
Xianbin Su, Yi Shi, Xin Zou, Zhao-Ning Lu, Gangcai Xie, Jean Y H Yang, Chong-Chao Wu, Xiao-Fang Cui, Kun-Yan He, Qing Luo, Yu-Lan Qu, Na Wang, Lan Wang, Ze-Guang Han
BACKGROUND: The differentiation and maturation trajectories of fetal liver stem/progenitor cells (LSPCs) are not fully understood at single-cell resolution, and a priori knowledge of limited biomarkers could restrict trajectory tracking. RESULTS: We employed marker-free single-cell RNA-Seq to characterize comprehensive transcriptional profiles of 507 cells randomly selected from seven stages between embryonic day 11.5 and postnatal day 2.5 during mouse liver development, and also 52 Epcam-positive cholangiocytes from postnatal day 3...
December 4, 2017: BMC Genomics
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