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https://www.readbyqxmd.com/read/28719148/high-expression-of-fundc1-predicts-poor-prognostic-outcomes-and-is-a-promising-target-to-improve-chemoradiotherapy-effects-in-patients-with-cervical-cancer
#1
Hailing Hou, Puchun Er, Jingjing Cheng, Xiuli Chen, Xiaofeng Ding, Yuwen Wang, Xi Chen, Zhiyong Yuan, Qingsong Pang, Ping Wang, Dong Qian
FUN14 domain containing 1 (FUNDC1) is an important molecule in receptor-dependent mitophagy. However, the roles of FUNDC1 in human cancer biology remain unknown. The aim of this study was to explore the expression and roles of FUNDC1 in cervical cancer. Immunohistochemistry and Western blotting were applied to detect the expression of FUNDC1, and small-hairpin RNA was applied to inhibit the expression of endogenous FUNDC1 in cervical cancer cells. MTT assays and Flow cytometric analysis were applied to examine cell proliferation and apoptosis...
July 18, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28718364/evaluation-of-epigenetic-inactivation-of-vascular-endothelial-growth-factor-receptors-in-head-and-neck-squamous-cell-carcinoma
#2
Yuki Misawa, Kiyoshi Misawa, Hideya Kawasaki, Atsushi Imai, Daiki Mochizuki, Ryuji Ishikawa, Shiori Endo, Masato Mima, Takeharu Kanazawa, Toshihide Iwashita, Hiroyuki Mineta
The aim of this study was to determine the methylation status of the genes encoding the vascular endothelial growth factor receptors and to evaluate the usefulness of VEGFR methylation as a prognostic indicator in head and neck squamous cell carcinoma. VEGFR messenger RNA expression and promoter methylation were examined in a panel of cell lines via quantitative reverse transcription and methylation-specific polymerase chain reaction, respectively. Promoter methylation was compared with clinical characteristics in 128 head and neck squamous cell carcinoma samples...
July 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28715477/the-rhlr-quorum-sensing-receptor-controls-pseudomonas-aeruginosa-pathogenesis-and-biofilm-development-independently-of-its-canonical-homoserine-lactone-autoinducer
#3
Sampriti Mukherjee, Dina Moustafa, Chari D Smith, Joanna B Goldberg, Bonnie L Bassler
Quorum sensing (QS) is a bacterial cell-to-cell communication process that relies on the production, release, and response to extracellular signaling molecules called autoinducers. QS controls virulence and biofilm formation in the human pathogen Pseudomonas aeruginosa. P. aeruginosa possesses two canonical LuxI/R-type QS systems, LasI/R and RhlI/R, which produce and detect 3OC12-homoserine lactone and C4-homoserine lactone, respectively. Here, we use biofilm analyses, reporter assays, RNA-seq studies, and animal infection assays to show that RhlR directs both RhlI-dependent and RhlI-independent regulons...
July 17, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28715399/integration-free-derivation-of-human-induced-pluripotent-stem-cells-using-laminin-521-matrix
#4
Elias Uhlin, Ana Marin Navarro, Harriet Rönnholm, Kelly Day, Malin Kele, Anna Falk
Xeno-free and fully defined conditions are key parameters for robust and reproducible generation of homogenous human induced pluripotent stem (hiPS) cells. Maintenance of hiPS cells on feeder cells or undefined matrices are susceptible to batch variances, pathogenic contamination and risk of immunogenicity. Utilizing the defined recombinant human laminin 521 (LN-521) matrix in combination with xeno-free and defined media formulations reduces variability and allows for the consistent generation of hiPS cells...
July 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28706128/transcriptome-analysis-reveals-a-role-for-the-endothelial-anp-gc-a-signaling-in-interfering-with-pre-metastatic-niche-formation-by-solid-cancers
#5
Takashi Nojiri, Miki Arai, Yutaka Suzuki, Motofumi Kumazoe, Takeshi Tokudome, Koichi Miura, Jun Hino, Hiroshi Hosoda, Mikiya Miyazato, Meinoshin Okumura, Shinpei Kawaoka, Kenji Kangawa
Cancer establishes a microenvironment called the pre-metastatic niche in distant organs where disseminated cancer cells can efficiently metastasize. Pre-metastatic niche formation requires various genetic factors. Previous studies suggest that inhibiting a single niche-factor is insufficient to completely block pre-metastatic niche formation especially in human patients. Here we show that the atrial natriuretic peptide (ANP), an endogenous hormone produced by the heart, inhibits pre-metastatic niche formation and metastasis of murine solid cancer models when pharmacologically supplied in vivo...
May 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28696914/micro-brushing-based-technique-to-gain-fresh-urothelial-cells-for-gene-expression-analysis
#6
Christoph Kuhn, Marie-Louise Lehmann, Alexander Kress, Michael C Truß, Matthias Hermes, Meinolf Blaszkewicz, Jan G Hengstler, Klaus Golka
The gold standard of saving fresh tissue in liquid nitrogen has some serious disadvantages in that this process is not available in daily medical routine practices even in many tumor centers. Our approach of a new minimally invasive technique is obtaining urothelial cells via micro-brushing the urinary bladder on the occasion of urological routine methods such as transurethral resection (TUR). Urothelial cells were obtained from 25 patients via two different micro-brushes from tumor tissue and from macroscopically healthy tissue during TUR...
July 11, 2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28696312/functional-characterization-of-human-pluripotent-stem-cell-derived-arterial-endothelial-cells
#7
Jue Zhang, Li-Fang Chu, Zhonggang Hou, Michael P Schwartz, Timothy Hacker, Vernella Vickerman, Scott Swanson, Ning Leng, Bao Kim Nguyen, Angela Elwell, Jennifer Bolin, Matthew E Brown, Ron Stewart, William J Burlingham, William L Murphy, James A Thomson
Here, we report the derivation of arterial endothelial cells from human pluripotent stem cells that exhibit arterial-specific functions in vitro and in vivo. We combine single-cell RNA sequencing of embryonic mouse endothelial cells with an EFNB2-tdTomato/EPHB4-EGFP dual reporter human embryonic stem cell line to identify factors that regulate arterial endothelial cell specification. The resulting xeno-free protocol produces cells with gene expression profiles, oxygen consumption rates, nitric oxide production levels, shear stress responses, and TNFα-induced leukocyte adhesion rates characteristic of arterial endothelial cells...
July 10, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28694731/detection-of-let-7-mirnas-in-urine-supernatant-as-potential-diagnostic-approach-in-non-metastatic-clear-cell-renal-cell-carcinoma
#8
Michal Fedorko, Jaroslav Juracek, Michal Stanik, Marek Svoboda, Alexandr Poprach, Tomas Buchler, Dalibor Pacik, Jan Dolezel, Ondrej Slaby
INTRODUCTION: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. MATERIALS AND METHODS: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled...
June 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/28694531/high-throughput-sequencing-of-rna-transcriptomes-in-ruditapes-philippinarum-identifies-genes-involved-in-osmotic-stress-response
#9
Hongtao Nie, Liwen Jiang, Peng Chen, Zhongming Huo, Feng Yang, Xiwu Yan
Ruditapes philippinarum, is an economically important marine bivalve species. The ability to cope with low salinity stress is quite important for the survival of aquatic species under natural conditions. In this study, the transcriptional response of the Manila clam to low salinity stress was characterized using RNA sequencing. The transcriptomes of a low salinity-treatment group (FRp1, FRp2), which survived under low salinity stress, and control group (SRp1, SRp2), which was not subjected to low salinity stress, were sequenced with the Illumina HiSeq platform...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28688308/a-pcr-free-electrochemical-method-for-messenger-rna-detection-in-cancer-tissue-samples
#10
Md Nazmul Islam, Vinod Gopalan, Md Hakimul Haque, Mostafa Kamal Masud, Md Shahriar Al Hossain, Yusuke Yamauchi, Nam-Trung Nguyen, Alfred King-Yin Lam, Muhammad J A Shiddiky
Despite having reliable and excellent diagnostic performances, the currently available messenger RNA (mRNA) detection methods mostly use enzymatic amplification steps of the target mRNA which is generally affected by the sample manipulations, amplification bias and longer assay time. This paper reports an amplification-free electrochemical approach for the sensitive and selective detection of mRNA using a screen-printed gold electrode (SPE-Au). The target mRNA is selectively isolated by magnetic separation and adsorbed directly onto an unmodified SPE-Au...
June 27, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28683444/replication-protein-a-3-is-associated-with-hepatocellular-carcinoma-tumorigenesis-and-poor-patient-survival
#11
Wenbo Xiao, Jun Zheng, Baoyong Zhou, Long Pan
BACKGROUND: Replication protein A (RPA) 3 is a subunit of the RPA protein complex, which functions in multiple processes of DNA metabolism. Dysregulation of RPA1 and RPA2 has been implicated in tumor progression in several cancer types. However, the function of RPA3 in hepatocellular carcinoma (HCC) tumorigenesis has not been elucidated. METHOD: In this study, we investigated the function of RPA3 in HCC development by stably knocking down its expression using short hairpin RNA (shRNA) in HepG2 cell line, followed by cell proliferation, colony formation, soft agar, and invasion assays...
July 7, 2017: Digestive Diseases
https://www.readbyqxmd.com/read/28683259/rna-seq-analysis-of-peripheral-blood-mononuclear-cells-reveals-unique-transcriptional-signatures-associated-with-disease-progression-in-dengue-patients
#12
Arup Banerjee, Shweta Shukla, Abhay Deep Pandey, Saptamita Goswami, Bhaswati Bandyopadhyay, Vishnampettai Ramachandran, Shukla Das, Arjun Malhotra, Amitesh Agarwal, Srima Adhikari, Mehebubar Rahman, Shatakshee Chatterjee, Nemai Bhattacharya, Nandita Basu, Priyanka Pandey, Vikas Sood, Sudhanshu Vrati
Patients infected with Dengue virus usually present a mild, self-limiting febrile dengue infection (DI) that occasionally leads to a potentially lethal complication, called the severe dengue (DS). The ability to identify the prognostic markers of DS could allow an improved disease intervention and management. To identify the transcriptional signatures associated with the dengue disease progression, we carried out the high-throughput sequencing of the RNA isolated from the peripheral blood mononuclear cells (PBMCs) of the dengue patients of varying severity and compared with that in the patients with other febrile illnesses (OFIs) or the healthy controls...
June 17, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/28680592/hif-prolyl-hydroxylase-phd3-regulates-translational-machinery-and-glucose-metabolism-in-clear-cell-renal-cell-carcinoma
#13
Petra Miikkulainen, Heidi Högel, Krista Rantanen, Tomi Suomi, Petri Kouvonen, Laura L Elo, Panu M Jaakkola
BACKGROUND: A key feature of clear cell renal cell carcinoma (ccRCC) is the inactivation of the von Hippel-Lindau tumour suppressor protein (pVHL) that leads to the activation of hypoxia-inducible factor (HIF) pathway also in well-oxygenated conditions. Important regulator of HIF-α, prolyl hydroxylase PHD3, is expressed in high amounts in ccRCC. Although several functions and downstream targets for PHD3 in cancer have been suggested, the role of elevated PHD3 expression in ccRCC is not clear...
2017: Cancer & Metabolism
https://www.readbyqxmd.com/read/28679691/metastatic-triple-negative-breast-cancer-patient-with-tp53-tumor-mutation-experienced-11-months-progression-free-survival-on-bortezomib-monotherapy-without-adverse-events-after-ending-standard-treatments-with-grade-3-adverse-events
#14
Tobias Meißner, Adam Mark, Casey Williams, Wolfgang E Berdel, Stephanie Wiebe, Andrea Kerkhoff, Eva Wardelmann, Timo Gaiser, Carsten Müller-Tidow, Philip Rosenstiel, Norbert Arnold, Brian Leyland-Jones, Andre Franke, Martin Stanulla, Michael Forster
A triple-negative breast cancer patient had no hereditary BRCA1, BRCA2, or TP53 risk variants. After exhaustion of standard treatments, she underwent experimental treatments and whole-exome sequencing of tumor, blood, and a metastasis. Well-tolerated experimental bortezomib monotherapy was administered for a progression-free period of 11 mo. After progression, treatments were changed and the exome data were evaluated, expanded with RNA and exome sequencing of a late-stage metastasis. In the final stage, eribulin alone and in combination with anthracyclines were administered...
July 2017: Cold Spring Harbor Molecular Case Studies
https://www.readbyqxmd.com/read/28679534/hsp70-s-rna-binding-and-mrna-stabilizing-activities-are-independent-of-its-protein-chaperone-functions
#15
Aparna Kishor, Elizabeth J F White, Aerielle E Matsangos, Zisui Yan, Bishal Tandukar, Gerald M Wilson
Hsp70 is a protein chaperone that prevents protein aggregation and aids protein folding by binding to hydrophobic peptide domains through a reversible mechanism directed by an ATPase cycle. However, Hsp70 also binds U-rich RNA including some AU-rich elements (AREs) that regulate the decay kinetics of select mRNAs and has recently been shown to bind and stabilize some ARE-containing transcripts in cells. Previous studies indicated that both the ATP- and peptide-binding domains of Hsp70 contributed to the stability of Hsp70:RNA complexes and that ATP might inhibit RNA recruitment...
July 5, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28678784/personalized-rna-mutanome-vaccines-mobilize-poly-specific-therapeutic-immunity-against-cancer
#16
Ugur Sahin, Evelyna Derhovanessian, Matthias Miller, Björn-Philipp Kloke, Petra Simon, Martin Löwer, Valesca Bukur, Arbel D Tadmor, Ulrich Luxemburger, Barbara Schrörs, Tana Omokoko, Mathias Vormehr, Christian Albrecht, Anna Paruzynski, Andreas N Kuhn, Janina Buck, Sandra Heesch, Katharina H Schreeb, Felicitas Müller, Inga Ortseifer, Isabel Vogler, Eva Godehardt, Sebastian Attig, Richard Rae, Andrea Breitkreuz, Claudia Tolliver, Martin Suchan, Goran Martic, Alexander Hohberger, Patrick Sorn, Jan Diekmann, Janko Ciesla, Olga Waksmann, Alexandra-Kemmer Brück, Meike Witt, Martina Zillgen, Andree Rothermel, Barbara Kasemann, David Langer, Stefanie Bolte, Mustafa Diken, Sebastian Kreiter, Romina Nemecek, Christoffer Gebhardt, Stephan Grabbe, Christoph Höller, Jochen Utikal, Christoph Huber, Carmen Loquai, Özlem Türeci
T cells directed against mutant neo-epitopes drive cancer immunity. However, spontaneous immune recognition of mutations is inefficient. We recently introduced the concept of individualized mutanome vaccines and implemented an RNA-based poly-neo-epitope approach to mobilize immunity against a spectrum of cancer mutations. Here we report the first-in-human application of this concept in melanoma. We set up a process comprising comprehensive identification of individual mutations, computational prediction of neo-epitopes, and design and manufacturing of a vaccine unique for each patient...
July 13, 2017: Nature
https://www.readbyqxmd.com/read/28671653/protein-trna-agarose-gel-retardation-assays-for-the-analysis-of-the-n-6-threonylcarbamoyladenosine-tcda-function
#17
Francisco J Fernández, Sara Gómez, Sergio Navas-Yuste, Miguel López-Estepa, M Cristina Vega
We demonstrate methods for the expression and purification of tRNA(UUU) in Escherichia coli and the analysis by gel retardation assays of the binding of tRNA(UUU) to TcdA, an N(6)-threonylcarbamoyladenosine (t(6)A) dehydratase, which cyclizes the threonylcarbamoyl side chain attached to A37 in the anticodon stem loop (ASL) of tRNAs to cyclic t(6)A (ct(6)A). Transcription of the synthetic gene encoding tRNA(UUU) is induced in E. coli with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and the cells containing tRNA are harvested 24 h post-induction...
June 21, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28671041/high-expression-of-rac1-is-correlated-with-partial-reversed-cell-polarity-and-poor-prognosis-in-invasive-ductal-carcinoma-of-the-breast
#18
Bingbing Liu, Jianhua Xiong, Guiqiu Liu, Jing Wu, Likun Wen, Qin Zhang, Chuanshan Zhang
The change of cell polarity is usually associated with invasion and metastasis. Partial reverse cell polarity in IDC-NOS may play a role in lymphatic tumor spread. Rac1 is a kind of polarity related protein. It plays an important role in invasion and metastasis in tumors. We here investigated the expression of Rac1 and partial reverse cell polarity status in breast cancer and evaluated their value for prognosis in breast cancer. The association of the expression of Rac1 and MUC-1 with clinicopathological parameters and prognostic significance was evaluated in 162 cases of IDC-NOS paraffin-embedded tissues by immunohistochemical method...
July 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28671021/upregulation-of-transferrin-receptor-1-induces-cholangiocarcinoma-progression-via-induction-of-labile-iron-pool
#19
Wassana Jamnongkan, Raynoo Thanan, Anchalee Techasen, Nisana Namwat, Watcharin Loilome, Piyapharom Intarawichian, Attapol Titapun, Puangrat Yongvanit
Labile iron pool is a cellular source of ions available for Fenton reactions resulting in oxidative stress. Living organisms avoid an excess of free irons by a tight control of iron homeostasis. We investigated the altered expression of iron regulatory proteins and iron discrimination in the development of liver fluke-associated cholangiocarcinoma. Additionally, the levels of labile iron pool and the functions of transferrin receptor-1 on cholangiocarcinoma development were also identified. Iron deposition was determined using the Prussian blue staining method in human cholangiocarcinoma tissues...
July 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28670387/down-regulation-of-long-non-coding-rna-afap1-as1-inhibits-tumor-cell-growth-and-invasion-in-lung-adenocarcinoma
#20
Yi Zhuang, Hanyi Jiang, Hui Li, Jinhong Dai, Yin Liu, Yan Li, Liyun Miao, Hourong Cai, Yonglong Xiao, Hongping Xia, Yongsheng Wang, Mingke Shi
An increasing number of deregulated long non-coding RNAs (lncRNA) have been implicated in cancer in humans, suggesting that lncRNAs may be involved in tumorigenesis or tumor progression. In previous investigations, lncRNA, AFAP1-AS1 has been found to be associated with several cancers, including nasopharyngeal carcinoma, lung cancer and esophageal adenocarcinoma. However, the function of AFAP1-AS1 in lung cancer has not been reported. In our present study, we found that AFAP1-AS1 was overexpressed in lung adenocarcinoma and associated with survival time...
2017: American Journal of Translational Research
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