Thanyavi Chinsuwan, Koichi Hirabayashi, Shuji Mishima, Aiko Hasegawa, Miyuki Tanaka, Hidemi Mochizuki, Akihito Shimoi, Takashi Murakami, Shigeki Yagyu, Kimihiro Shimizu, Yozo Nakazawa
Epidermal growth factor receptor (EGFR) is overexpressed in various cancers, including non-small cell lung cancer (NSCLC), and in some somatic cells at a limited level, rendering it an attractive antitumor target. In this study, we engineered chimeric antigen receptor (CAR)-T cells using the piggyBac transposon system, autologous artificial antigen-presenting cells, and natural ligands of EGFR. We showed that this approach yielded CAR-T cells with favorable phenotypes and CAR positivity. They exhibited potent antitumor activity against NSCLC both in vitro and in vivo ...
December 19, 2023: Molecular Therapy Oncolytics