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Non structural protein 3

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https://www.readbyqxmd.com/read/28097814/detection-and-molecular-characterization-of-foot-and-mouth-disease-viruses-from-outbreaks-in-some-states-of-northern-nigeria-2013-2015
#1
D O Ehizibolo, A Haegeman, A R De Vleeschauwer, J U Umoh, H M Kazeem, E C Okolocha, K De Clercq, S Van Borm
Control measures for foot and mouth disease (FMD) in Nigeria have not been implemented due to the absence of locally produced vaccines and risk-based analysis resulting from insufficient data on the circulating FMD virus (FMDV) serotypes/strains. In 2013-2015, blood and epithelial samples were collected from reported FMD outbreaks in four states (Kaduna, Kwara, Plateau and Bauchi) in northern Nigeria. FMDV non-structural protein (NSP) seroprevalence for the outbreaks was estimated at 80% (72 of 90) and 70% (131 of 188) post-outbreak...
January 17, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28078551/the-proteolysis-adaptor-nbla-binds-to-the-n-terminus-of-%C3%AE-phycocyanin-implications-for-the-mechanism-of-phycobilisome-degradation
#2
Amelia Y Nguyen, William P Bricker, Hao Zhang, Daniel A Weisz, Michael L Gross, Himadri B Pakrasi
Phycobilisome (PBS) complexes are massive light-harvesting apparati in cyanobacteria that capture and funnel light energy to the photosystem. PBS complexes are dynamically degraded during nutrient deprivation, which causes severe chlorosis, and resynthesized during nutrient repletion. PBS degradation occurs rapidly after nutrient step down, and is specifically triggered by non-bleaching protein A (NblA), a small proteolysis adaptor that facilitates interactions between a Clp chaperone and phycobiliproteins...
January 11, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28077807/folding-of-apomyoglobin-analysis-of-transient-intermediate-structure-during-refolding-using-quick-hydrogen-deuterium-exchange-and-nmr
#3
Chiaki Nishimura
The structures of apomyoglobin folding intermediates have been widely analyzed using physical chemistry methods including fluorescence, circular dichroism, small angle X-ray scattering, NMR, mass spectrometry, and rapid mixing. So far, at least two intermediates (on sub-millisecond- and millisecond-scales) have been demonstrated for apomyoglobin folding. The combination of pH-pulse labeling and NMR is a useful tool for analyzing the kinetic intermediates at the atomic level. Its use has revealed that the latter-phase kinetic intermediate of apomyoglobin (6 ms) was composed of helices A, B, G and H, whereas the equilibrium intermediate, called the pH 4 molten-globule intermediate, was composed mainly of helices A, G and H...
2017: Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
https://www.readbyqxmd.com/read/28075068/binding-and-processing-of-%C3%AE-lactam-antibiotics-by-the-transpeptidase-ldtmt2-from-mycobacterium-tuberculosis
#4
Eva Maria Steiner, Gunter Schneider, Robert Schnell
β-lactam antibiotics represent a novel direction in the chemotherapy of tuberculosis that brings the peptidoglycan layer of the complex mycobacterial cell wall in focus as therapeutic target. Peptidoglycan stability in Mycobacterium tuberculosis especially during infection relies on the non-conventional peptide cross-links formed by L,D-transpeptidases. These enzymes are known to be inhibited by β-lactams, primarily carbapenems, leading to a stable covalent modification at the enzyme active site. A panel of sixteen β-lactams antibiotics was characterized by inhibition kinetics, mass spectrometry and x-ray crystallography to identify efficient compounds and study their action on the essential transpeptidase LdtMt2 ...
January 11, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28073065/in-silico-approach-to-identify-non-synonymous-snps-in-human-obesity-related-gene-mc3r-melanocortin-3-receptor
#5
Rajan Kumar Singh, Kulandaivelu Mahalingam
The melanocortin-3-receptor (MC3R) is a novel gene candidate for human obesity, which involved in controlling the energy homeostasis and food intake behavior. The main aim behind this work is to investigate the potentially deleterious non-synonymous single nucleotide polymorphisms (nsSNPs) in obesity related gene MC3R by using six computational tools viz., PolyPhen, I-Mutant, PROVEAN, SIFT, PANTHER and PhD-SNP. In our study, we predicted eight nsSNPs i.e., rs74315393 (Ile146Asn), rs368205448 (Asp121Tyr), rs143321797 (Phe45Ser), rs17847261 (Cys274Ser), rs144166442 (Pro257His), rs370533946 (Leu224Pro), rs371354428 (Pro72Leu) and rs373708098 (Gly249Ser) found to be potentially deleterious...
December 29, 2016: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28072537/a-qm-mm-study-of-substituent-and-solvent-effects-on-the-excited-state-dynamics-of-the-photoactive-yellow-protein-chromophore
#6
Francisco Fernández García-Prieto, Aurora Muñoz-Losa, Ignacio Fdez Galván, M Luz Sánchez, Manuel Ángel Aguilar, M Elena Martín
Substituent and solvent effects on the excited state dynamics of the Photoactive Yellow Protein chromophore are studied using the average solvent electrostatic potential from molecular dynamics (ASEP/MD) method. Four molecular models were considered: the ester and thioester derivatives of the p-coumaric acid anion and their methyla ted derivatives. We found that the solvent produces dramatic modifications on the free energy profile of the S1 state: 1) Two twisted structures that are minima in gas phase could not be located in aqueous solution...
January 10, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28065508/kobuviral-non-structural-3a-proteins-act-as-molecular-harnesses-to-hijack-the-host-acbd3-protein
#7
Martin Klima, Dominika Chalupska, Bartosz Różycki, Jana Humpolickova, Lenka Rezabkova, Jan Silhan, Adriana Baumlova, Anna Dubankova, Evzen Boura
Picornaviruses are small positive-sense single-stranded RNA viruses that include many important human pathogens. Within the host cell, they replicate at specific replication sites called replication organelles. To create this membrane platform, they hijack several host factors including the acyl-CoA-binding domain-containing protein-3 (ACBD3). Here, we present a structural characterization of the molecular complexes formed by the non-structural 3A proteins from two species of the Kobuvirus genus of the Picornaviridae family and the 3A-binding domain of the host ACBD3 protein...
December 21, 2016: Structure
https://www.readbyqxmd.com/read/28063412/spectral-characterisation-antiviral-activities-in-silico-admet-and-molecular-docking-of-the-compounds-isolated-from-tectona-grandis-to-chikungunya-virus
#8
Sangeetha K, Indu Purushothaman, Rajarajan S
Chikungunya infection is treated symptomatically with antipyretics and anti-inflammatory drugs without any specific antiviral drug till date. The lack of an approved antiviral drug and the emergence of virulent strains after 2006 epidemics emphasize the need for the development of potential antiviral drugs to Chikungunya virus. Hence, we studied the antiviral activity of the extracts and compounds isolated from Tectona grandis leaves to both the Asian and East central South African strains of Chikungunya virus...
January 4, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28062795/silac-based-proteomics-of-primary-human-kidney-cells-reveals-a-novel-link-between-male-sex-hormones-and-impaired-energy-metabolism-in-diabetic-kidney-disease
#9
Sergi Clotet, Maria Jose Soler, Marta Riera, Julio Pascual, Fei Fang, Joyce Zhou, Ihor Batruch, Stella K Vasiliou, Apostolos Dimitromanolakis, Clara Barrios, Eleftherios Diamandis, James Scholey, Ana Konvalinka
Male sex predisposes to many kidney diseases. Considering that androgens exert deleterious effects in a variety of cell types within kidney, we hypothesized that dihydrotestosterone (DHT) would alter the biology of the renal tubular cell by inducing changes in the proteome. We employed stable isotope labeling with amino acids (SILAC) in an indirect spike-in fashion to accurately quantify the proteome in DHT- and 17β-estradiol (EST)-treated human proximal tubular epithelial cells (PTEC). Of the 5043 quantified proteins, 76 were differentially regulated...
January 4, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28054091/constrained-evolution-of-a-bispecific-enzyme-lessons-for-biocatalyst-design
#10
E Sugrue, C Scott, C J Jackson
One of the central goals of protein design and engineering is to be able to accurately predict the effects of a mutation on stability and activity. However, the genetic context into which mutations are introduced can lead to complex interactions between the mutation and other amino acids and unpredictable, non-additive, effects. This phenomenon is known as intramolecular epistasis and has been shown to restrict evolutionary paths through laboratory directed evolution experiments and ancestral protein reconstruction, but has rarely been studied at a quantitative level in naturally evolving enzymes...
January 5, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28052760/evaluation-of-the-importance-of-fever-with-respect-to-dengue-prognosis-according-to-the-2009-who-classification-a-retrospective-study
#11
Caroline Tukasan, Nathália Barbosa Furlan, Cássia Fernanda Estofolete, Maurício Lacerda Nogueira, Natal Santos da Silva
BACKGROUND: The 2009 revised World Health Organization (WHO) guidelines for dengue describe fever as the core symptom. Accordingly, the diagnosis of non-febrile patients is complicated. The aim of this study was to evaluate the importance of fever in patients with dengue according to the 2009 revised WHO classification. METHODS: In this study, we assessed 30,670 dengue cases using enzyme-linked immunosorbent assay, detection of the non-structural protein 1, or polymerase chain reaction for diagnostic confirmation...
January 4, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28051862/combined-utilization-of-1-h-nmr-ir-and-theoretical-calculations-to-elucidate-the-conformational-preferences-of-some-l-histidine-derivatives
#12
Carolyne B Braga, Roberto Rittner
The conformational preferences of amino acids and their derivatives have been the subject of many investigations, because protein folding pathways that determine three-dimensional geometries are primarily restricted by the conformational space of each amino acid residue. Here we systematically describe the conformational behavior of l-histidine methyl ester (His-OMe) and its N-acetylated derivative (Ac-His-OMe) in the isolated phase and in solution. To this end, we employed spectroscopic techniques ((1)H NMR and IR), supported by quantum chemical calculations...
January 13, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28044000/painful-locking-of-the-wrist-in-a-patient-with-pseudoachondroplasia-confirmed-by-comp-mutation
#13
Hirokazu Ideta, Shigeharu Uchiyama, Masanori Hayashi, Tomoki Kosho, Yukio Nakamura, Hiroyuki Kato
We report the case of a 40-year-old woman with pseudoachondroplasia (PSACH), with a heterozygous mutation (c.806A > G, p.Asp269Gly) located in the Type 3 repeats domain of the cartilage oligomeric matrix protein gene, who complained of the unusual symptom of painful locking of the wrist. Her condition was caused by a non-traumatic enlargement of the extensor carpi radialis longus (ECRL) and brevis (ECRB) tendons along with bulbous swelling of the synoviums around them. Surgical treatment resolved these unusual tendon-related symptoms...
January 2, 2017: Journal of Surgical Case Reports
https://www.readbyqxmd.com/read/28042883/utility-of-dengue-ns1-antigen-rapid-diagnostic-test-for-use-in-difficult-to-reach-areas-and-its-comparison-with-dengue-ns1-elisa-and-qrt-pcr
#14
Mohan K Shukla, Neeru Singh, Ravendra K Sharma, Pradip V Barde
The objective of this study was to demonstrate the utility of dengue virus (DENV) non structural protein 1 (NS1) based rapid diagnostic test (RDT) for use in tribal and difficult to reach areas for early dengue (DEN) diagnosis in acute phase patients and evaluate its sensitivity and specificity against DENV NS1 enzyme linked immune sorbent assay (ELISA) and real time reverse transcriptase polymerase chain reaction (qRT-PCR) The DENV NS1 RDT was used for preliminary diagnosis during outbreaks in difficult to reach rural and tribal areas...
January 2, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28039692/biological-characteristics-of-ccn-proteins-in-tumor-development
#15
Stephany C Barreto, Amitabha Ray, Elena G Geromichalou, Page Ag Edgar
The extracellular membrane (ECM) is no longer regarded as inert, rather it has multiple versatile physiologic functions. Its diverse composition is implicated in each step of cancer progression including inflammation, angiogenesis, tumor invasion and metastasis. In addition to structural proteins, the ECM also contains a family of non-structural proteins called matricellular proteins. The six secreted CCN proteins, which belong to the matricellular protein family, include the following: Cyr61 (CCN1), CTGF (CCN2), Nov (CCN3), WISP- 1 (CCN4), WISP-2 (CCN5) and WISP-3(CCN6)...
November 2016: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28035002/structural-insights-into-c-myc-interacting-zinc-finger-protein-1-miz-1-delineate-domains-required-for-dna-scanning-and-sequence-specific-binding
#16
Mikaël Bédard, Vincent Roy, Martin Montagne, Pierre Lavigne
c-Myc interacting zinc finger protein-1 (Miz-1) is a poly-Cys2His2 zinc finger (ZF) transcriptional regulator of many cell cycle genes. A Miz-1 DNA sequence consensus has recently been identified and has also unveiled Miz-1 functions in other cellular processes, underscoring its importance in the cell. Miz-1 contains 13 ZFs, but it is unknown why Miz-1 has so many ZFs and whether they recognize and bind DNA sequences in a typical fashion. Here, we used NMR to deduce the role of Miz-1 ZFs 1 to 4 in detecting the Miz-1 consensus sequence and preventing non-specific DNA binding...
December 29, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28033959/assessment-of-binding-properties-of-actinomycin-d-to-21nt-dna-segment-of-hmgb1-gene-promoter-using-spectroscopic-and-calorimetric-techniques
#17
Neelam Lohani, Himanshu Narayan Singh, Moganty R Rajeswari
HMGB1 (High mobility group box 1) protein has been an attractive therapeutic target for drug designing in various human cancers and inflammatory diseases. Overexpression of HMGB1 has been associated with all the seven hallmark of cancer. Actinomycin -D (Act-D) is a classical anticancer drug that binds GC-rich DNA with high affinity to cause transcription inhibition. In the present study we utilized the 21 nucleotide G rich DNA fragment in the regulatory region of hmgb1 gene for its ability to bind with Act-D...
December 29, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28031251/expression-and-biochemical-characterization-and-substrate-specificity-of-the-fucoidanase-from-formosa-algae
#18
Artem S Silchenko, Nadezhda E Ustyuzhanina, Mikhail I Kusaykin, Vadim B Krylov, Alexander S Shashkov, Andrey S Dmitrenok, Roza V Usoltseva, Anastasiya O Zueva, Nikolay E Nifantiev, Tatyana N Zvyagintseva
A gene that encodes fucoidanase ffa2 in the marine bacterium Formosa algae strain KMM 3553(T) was cloned, and the protein (FFA2) was produced in Escherichia coli Recombinant fucoidanase FFA2 was purified, and the biochemical properties of this enzyme were studied. The amino acid sequence of FFA2 showed 57% identity with known fucoidanase FcnA from Mariniflexile fucanivorans. The mass of the gene product FFA2 is 101.2 kDa (918 amino acid residues). Sequence analysis has revealed that fucoidanase FFA2 belongs to the GH107 (CAZy) family...
December 28, 2016: Glycobiology
https://www.readbyqxmd.com/read/28027456/immunohistochemical-localization-of-ionotropic-glutamate-receptors-in-the-rat-red-nucleus
#19
Zehra Minbay, Sema Serter Kocoglu, Duygu Gok Yurtseven, Ozhan Eyigor
In this study, we aimed to determine the presence as well as the diverse distribution of N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptor subunits in the rat red nucleus. Using adult Sprague-Dawley rats as the experimental animals, immunohistochemistry was performed on 30 µm thick coronal brain sections with antibodies against α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (GluA1-4), kainate (GluK1, GluK2/3, and GluK5), and NMDA (GluN1 and GluN2A) receptor subunits. The results showed that all ionotropic glutamate receptor subunits are expressed in the red nucleus...
December 21, 2016: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28019723/therapeutical-potential-of-deregulated-lysine-methyltransferase-smyd3-as-a-safe-target-for-novel-anticancer-agents
#20
Gurukumari Rajajeyabalachandran, Swetha Kumar, Thanabal Murugesan, Shanthi Ekambaram, Ramya Padmavathy, Sooriya Kumar Jegatheesan, Ramesh Mullangi, Sriram Rajagopal
SET and MYND domain containing-3 (SMYD3) is a member of the lysine methyltransferase family of proteins, and plays an important role in the methylation of various histone and non-histone targets. Proper functioning of SMYD3 is very important for the target molecules to determine their different roles in chromatin remodeling, signal transduction and cell cycle control. Due to the abnormal expression of SMYD3 in tumors, it is projected as a prognostic marker in various solid cancers. Areas covered: Here we elaborate on the general information, structure and the pathological role of SMYD3 protein...
December 26, 2016: Expert Opinion on Therapeutic Targets
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