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Leukocyte migration

Valeryi K Lishko, Valentin P Yakubenko, Tatiana P Ugarova, Nataly P Podolnikova
Platelet Factor 4 (PF4) is one of the most abundant cationic proteins secreted from α-granules of activated platelets. Based on its structure, PF4 was assigned to the CXC family of chemokines and has been shown to have numerous effects on myeloid leukocytes. However, the receptor for PF4 remains unknown. Here, we demonstrate that PF4 induces leukocyte responses through the integrin Mac-1 (αM β2 , CD11b/CD18). Human neutrophils, monocytes, U937 monocytic and HEK293 cells expressing Mac-1 strongly adhered to immobilized PF4 in a concentration-dependent manner...
March 14, 2018: Journal of Biological Chemistry
Hao Sun, Frederic Lagarrigue, Alexandre R Gingras, Zhichao Fan, Klaus Ley, Mark H Ginsberg
Integrin activation regulates adhesion, extracellular matrix assembly, and cell migration, thereby playing an indispensable role in development and in many pathological processes. A proline mutation in the central integrin β3 transmembrane domain (TMD) creates a flexible kink that uncouples the topology of the inner half of the TMD from the outer half. In this study, using leukocyte integrin α4β7, which enables development of gut-associated lymphoid tissue (GALT), we examined the biological effect of such a proline mutation and report that it impairs agonist-induced talin-mediated activation of integrin α4β7, thereby inhibiting rolling lymphocyte arrest, a key step in transmigration...
March 13, 2018: Journal of Cell Biology
Pablo J Sáez, Lucie Barbier, Rafaele Attia, Hawa-Racine Thiam, Matthieu Piel, Pablo Vargas
In multicellular organisms, cell migration is a complex process. Examples of this are observed during cell motility in the interstitial space, full of extracellular matrix fibers, or when cells pass through endothelial layers to colonize or exit specific tissues. A common parameter for both situations is the fast adaptation of the cellular shape to their irregular landscape. In this chapter, we describe two methods to study cell migration in complex environments. The first one consists in a multichamber device for the visualization of cell haptotaxis toward the collagen-binding chemokine CCL21...
2018: Methods in Molecular Biology
Xiao Wang, Daniel Irimia
Neutrophils are the most abundant leukocytes in blood serving as the first line of host defense in tissue damage and infections. Upon activation by chemokines released from pathogens or injured tissues, neutrophils migrate through tissues toward sites of infections along the chemokine gradients, in a process named chemotaxis. Studying neutrophil chemotaxis using conventional tools, such as a transwell assay, often requires isolation of neutrophils from whole blood. This process requires milliliters of blood, trained personnel, and can easily alter the ability of chemotaxis...
2018: Methods in Molecular Biology
Penny E Morton, Maddy Parsons
Migration of leukocytes through epithelial monolayers represents an essential step in the generation of an inflammatory response and is often seen in inflammatory conditions such as Crohn's disease (Matthews et al., Toxicol Pathol 42:91-98, 2014) and asthma (Lambrecht and Hammad, Nat Med 18:684-692, 2012). Transepithelial migration involves adhesion to the basal surface of the epithelium before migration through the epithelial cell layer to the apical surface. Analyzing this process can present a technical challenge due to complications of using a coculture model and trying to recapitulate an intact monolayer...
2018: Methods in Molecular Biology
Tamires Rocha Falcão, Aurigena Antunes de Araújo, Luiz Alberto Lira Soares, Rhayanne Thaís de Moraes Ramos, Isabelle Cristinne Ferraz Bezerra, Magda Rhayanny Assunção Ferreira, Manoel André de Souza Neto, Maria Celeste Nunes Melo, Raimundo Fernandes de Araújo, Andreza Conceição Véras de Aguiar Guerra, Juliana Silva de Medeiros, Gerlane Coelho Bernardo Guerra
BACKGROUND: This study showed phytochemical composition and evaluates the anti-inflammatory, and analgesic activities of crude extract (CE) and fractions from E. uniflora Linn leaves. METHODS: Polyphenols present in crude extract (CE), in aqueous fraction (AqF), and ethyl acetate (EAF) treated fractions from E. uniflora Linn leaves were shown by chromatographic analysis in order to conduct a phytochemical characterization. Antibacterial activity was evaluated based on minimum inhibitory concentrations (MICs) determined using the agar dilution method...
March 9, 2018: BMC Complementary and Alternative Medicine
Hua Ye, Shijun Xiao, Xiaoqing Wang, Zhiyong Wang, Zhengshi Zhang, Chengke Zhu, Bingjie Hu, Changhuan Lv, Shuming Zheng, Hui Luo
Schizothorax prenanti (S. prenanti) is an indigenous fish species and is popularly cultured in southwestern China. In recent years, intensive farming of S. prenanti and water quality deterioration has increased the susceptibility of this fish to various pathogens, including Aeromonas hydrophila (A. hydrophila), which has caused severe damage to S. prenanti production. However, the understanding of molecular immune response of S. prenanti to A. hydrophila infection is still lacking. In order to better comprehend the S...
March 8, 2018: Marine Biotechnology
Tomoyuki Odaka, Hiroaki Suetake, Tomoki Maeda, Toshiaki Miyadai
Recently, mammalian basophils have been highlighted as having roles in allergy and antiparasitic immunity; however, there is little information about the functions and evolutionary origin of basophils, because they are the least abundant leukocyte in most vertebrates. In this study, we characterized the teleost basophils that are abundant in the peripheral blood of fugu ( Takifugu rubripes ). Fugu basophils have two distinct granules: reddish-purple and dark violet ones. Teleost fish do not have IgG and IgE, but we found that fugu IgM bound on the surface of the basophils, and the cross-linked IgM induced degranulation of both types of granules...
March 7, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Mingxing Yang, Maxie Kohler, Tina Heyder, Helena Forsslund, Hilde K Garberg, Reza Karimi, Johan Grunewald, Frode S Berven, C Magnus Sköld, Åsa M Wheelock
BACKGROUND: Smoking represents a significant risk factor for many chronic inflammatory diseases, including chronic obstructive pulmonary disease (COPD). METHODS: To identify dysregulation of specific proteins and pathways in bronchoalveolar lavage (BAL) cells associated with smoking, isobaric tags for relative and absolute quantitation (iTRAQ)-based shotgun proteomics analyses were performed on BAL cells from healthy never-smokers and smokers with normal lung function from the Karolinska COSMIC cohort...
March 8, 2018: Respiratory Research
Jing Han, Yue Li, Xiuli Liu, Tongrong Zhou, Haijing Sun, Paul Edwards, Hua Gao, Fu-Shin Yu, Xiaoxi Qiao
The oral anti-diabetic drug metformin has been found to reduce cardiovascular complications independent of glycemic control in diabetic patients. However, its role in diabetic retinal microvascular complications is not clear. This study is to investigate the effects of metformin on retinal vascular endothelium and its possible mechanisms, regarding two major pathogenic features of diabetic retinopathy: angiogenesis and inflammation. In human retinal vascular endothelial cell culture, metformin inhibited various steps of angiogenesis including endothelial cell proliferation, migration, and tube formation in a dose-dependent manner...
2018: PloS One
Ai-Ling Tian, MingMin Lu, Guillermo Calderón-Mantilla, Evangelia Petsalaki, Tania Dottorini, XiaoWei Tian, YuJian Wang, Si-Yang Huang, Jun-Ling Hou, XiangRui Li, Hany M Elsheikha, Xing-Quan Zhu
BACKGROUND: The molecular structure of Fasciola gigantica 14-3-3 protein has been characterized. However, the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e), and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs). METHODS: rFg14-3-3e protein was expressed in Pichia pastoris...
March 6, 2018: Parasites & Vectors
Gang Chen, Ming Yan, Rong Rong Li, Wan Tao Chen
OBJECTIVE: To investigate the Sonic Hedgehog (SHH) signalling molecules and activated leukocyte cell adhesion molecule (ALCAM) expression in the mechanisms regulating invasion and metastasis in oral squamous cell carcinoma (OSCC). METHODS: The expressions of SHH signalling molecules Gli family zinc finger 1/2 (Gli1/Gli2), as well as ALCAM expression, was analysed in 101 OSCC patients by immunohistochemistry. The potential relationship between Gli1/Gli2 and ALCAM in regard to invasion and metastasis were studied by western blot, invasion and wound-healing assays...
2018: Chinese Journal of Dental Research
Susana Beceiro, Attila Pap, Zsolt Czimmerer, Tamer Sallam, Jose A Guillén, Germán Gallardo, Cynthia Hong, Noelia A-Gonzalez, Carlos Tabraue, Mercedes Diaz, Felix Lopez, Jonathan Matalonga, Annabel F Valledor, Pilar Dominguez, Carlos Ardavin, Cristina Delgado-Martin, Santiago Partida-Sanchez, Jose Luis Rodriguez-Fernandez, Laszlo Nagy, Peter Tontonoz, Antonio Castrillo
The liver X receptors (LXRs) are ligand-activated nuclear receptors with established roles in the maintenance of lipid homeostasis in multiple tissues. LXRs exert additional biological functions as negative regulators of inflammation, particularly in macrophages. However, the transcriptional responses controlled by LXRs in other myeloid cells, such as dendritic cells (DC), are still poorly understood. Here we used gain- and loss-of-function models to characterize the impact of LXR deficiency on DC activation programs...
March 5, 2018: Molecular and Cellular Biology
Lucas Olivo Martins Pereira, Wagner Vilegas, Marcelo Marucci Pereira Tangerina, Karuppusamy Arunachalam, Sikiru Olaitan Balogun, Paulo Eduardo Orlandi Matos, Domingos Tabajara de Oliveira Martins
ETHNOPHARMACOLOGICAL IMPORTANCE: Lafoensia pacari A. St.-Hil., (Lythraceae) is a native tree of Brazilian Cerrado and commonly known in Brazil as "mangava-brava". Its leaves are used in Brazilian folk medicine in wound healing, cutaneous mycoses, and in the treatment of gastritis and ulcers. AIM OF THE STUDY: The present study was designed to evaluate the wound healing activity and mechanism of action of the hydroethanolic extract of Lafoensia pacari A...
February 28, 2018: Journal of Ethnopharmacology
José Ivan Marques, Jovelina Samara Ferreira Alves, Manoela Torres-Rêgo, Allanny Alves Furtado, Emerson Michell da Silva Siqueira, Eder Galinari, Daline Fernandes de Souza Araújo, Gerlane Coelho Bernardo Guerra, Eduardo Pereira de Azevedo, Matheus de Freitas Fernandes-Pedrosa, Silvana Maria Zucolotto
Tabernaemontana catharinensis (Apocynaceae) has been popularly used by folk medicine because of its anti-inflammatory, analgesic, and antiophidic properties. This study aims to analyze the flavonoids composition of the hydroethanolic extract and of the ethyl acetate (EtOAc) and butanol (BuOH) fractions of T. catharinensis leaves, as well as to evaluate their anti-inflammatory activity using in vivo models. The phytochemical profile, determined by High-Performance Liquid Chromatography-High-Resolution Electrospray Ionization-Mass Spectrometry (HPLC-HRESI-MS), showed the presence of flavonoids mainly having an isorhamnetin nucleus...
February 24, 2018: International Journal of Molecular Sciences
Marco Alonso Martinez-Guzman, Anabell Alvarado-Navarro, Vidal Delgado-Rizo, Alejandra Garcia-Orozco, Jorge Arturo Mayorga-Rodríguez, Ana Laura Pereira-Suarez, Mary Fafutis-Morris
Leprosy is a chronic disease caused by Mycobacterium leprae that affects the skin and peripheral nerves. It may present as one of two distinct poles: the self-limiting tuberculoid leprosy and the highly infectious lepromatous leprosy (LL) characterized by M. leprae -specific absence of cellular immune response. The pro-inflammatory cytokine macrophage migration inhibitory factor (MIF) enhance the bactericide activities of macrophages after interaction with its receptor, CD74. Importantly, MIF also possesses chemoattractant properties, and it is a key factor in situ for the activation of macrophages and in blood to promote leukocytes migration...
2018: Frontiers in Immunology
Melanie Salvermoser, Robert Pick, Ludwig T Weckbach, Annette Zehrer, Phillip Löhr, Maik Drechsler, Markus Sperandio, Oliver Soehnlein, Barbara Walzog
Neutrophil extravasation and interstitial migration are important steps during the recruitment of neutrophils to sites of inflammation. In the present study, we addressed the functional importance of the unconventional class I myosin 1f (Myo1f) for neutrophil trafficking during acute inflammation. In contrast to leukocyte rolling and adhesion, the genetic absence of Myo1f severely compromised neutrophil extravasation into the inflamed mouse cremaster tissue when compared to Myo1f+/+ mice as studied by intravital microscopy...
February 27, 2018: Blood
M A Chelombitko, O A Averina, T V Vasilyeva, O Yu Pletiushkina, E N Popova, A V Fedorov, B V Chernyak, V S Shishkina, O P Ilinskaya
The therapeutic effect of mitochondria-targeted antioxidant 10-(6´-plastoquinonyl)decyltriphenylphosphonium bromide (SkQ1) in experimental models of acute inflammation and wound repair has been shown earlier. It was suggested that the antiinflammatory activity of SkQ1 is related to its ability to suppress inflammatory activation of the vascular endothelium and neutrophil migration into tissues. Here, we demonstrated that SkQ1 inhibits activation of mast cells (MCs) followed by their degranulation and histamine release in vivo and in vitro...
December 2017: Biochemistry. Biokhimii︠a︡
Yunzhong Zhang, Jing Feng, Jizhen Cui, Guozheng Yang, Xianai Zhu
The present study investigated the roles of pre-B cell leukemia transcription factor 3 (PBX3) in sepsis. Reverse transcription-quantitative polymerase chain reaction and western blot analysis indicated that overexpression of the PBX 3'‑untranslated region (UTR) promoted high mobility group box 1 (HMGB1) protein expression in human umbilical vein endothelial cells (HUVECs) (P<0.01). Furthermore, post‑treatment of PBX3 small interfering (si)RNA suppressed lipopolysaccharide (LPS)‑mediated HMGB1 release and attenuated HMGB1‑mediated hyperpermeability and leukocyte migration in HUVECs and septic mice (P<0...
February 15, 2018: Molecular Medicine Reports
Zhuo-Ma Luoreng, Xing-Ping Wang, Chu-Gang Mei, Lin-Sen Zan
MicroRNAs (miRNAs) play crucial roles in regulating innate and adaptive immunity in humans and animals. Infection with E. coli or S. aureus can cause inflammation of the mammary glands, which results in significant economic losses in dairy cattle. However, the regulatory mechanisms of miRNAs in response to E. coli or S. aureus infection in bovine mammary glands have not been thoroughly explored. To discover the differential expression of miRNA in bovine mammary gland challenged with E. coli or S. aureus, we performed miRNA sequencing on tissue samples...
2018: International Journal of Biological Sciences
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