Dieter Blottner, Manuela Moriggi, Gabor Trautmann, Sandra Furlan, Katharina Block, Martina Gutsmann, Enrica Torretta, Pietro Barbacini, Daniele Capitanio, Joern Rittweger, Ulrich Limper, Pompeo Volpe, Cecilia Gelfi, Michele Salanova
Long-duration mission (LDM) astronauts from the International Space Station (ISS) (>180 ISS days) revealed a close-to-normal sarcolemmal nitric oxide synthase type-1 (NOS1) immunoexpression in myofibers together with biochemical and quantitative qPCR changes in deep calf soleus muscle. Nitro-DIGE analyses identified functional proteins (structural, metabolic, mitochondrial) that were over-nitrosylated post- vs. preflight. In a short-duration mission (SDM) astronaut (9 ISS days), s-nitrosylation of a nodal protein of the glycolytic flux, specific proteins in tricarboxylic acid (TCA) cycle, respiratory chain, and over-nitrosylation of creatine kinase M-types as signs of impaired ATP production and muscle contraction proteins were seen...
April 2, 2024: Antioxidants (Basel, Switzerland)