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Ritesh K Srivastava, Changzhao Li, Zhiping Weng, Anupam Agarwal, Craig A Elmets, Farrukh Afaq, Mohammad Athar
Arsenicals are painful, inflammatory and blistering causing agents developed as chemical weapons in World War I/II. However, their large stockpiles still exist posing threat to public health. Phenylarsine oxide (PAO), a strong oxidant and a prototype arsenical is tested for its suitability to defining molecular mechanisms underlying arsenicals-mediated tissue injury. Topically applied PAO induces cutaneous erythema, edema and micro-blisters. These gross inflammatory responses were accompanied by the enhanced production of pro-inflammatory cytokines, ROS and unfolded protein response (UPR) signaling activation...
October 11, 2016: Scientific Reports
Atsushi Watanabe, Jiro Kumaki
Precise morphology control of ultrathin films is one of the important issues in nanotechnology. To this end, we describe that various controlled morphologies of hierarchical phase separation can be attained using poly(methyl methacrylate)-b-poly(n-butyl acrylate) (PMMA-b-PBA) monolayers spread on a water surface. At a low surface pressure, they were miscible, but upon compression, phase separated with a monolayer of the major component block spreading on the water surface, on top of which the minor component block separated out (hierarchical phase separation)...
October 4, 2016: Journal of Colloid and Interface Science
Muhamad S Musa, Amir H Milani, Peter Shaw, Gareth Simpson, Peter A Lovell, Elizabeth Eaves, Nigel Hodson, Brian R Saunders
In this study we investigate the structure-mechanical property relationships for nanostructured ionomer films containing ionically crosslinked core-shell polymer nanoparticles based on poly(n-butyl acrylate) (PBA). Whilst nanostructured ionomer films of core-shell nanoparticles have been previously shown to have good ductility [Soft Matter, 2014, 10, 4725], the modulus values were modest. Here, we used BA as the primary monomer to construct core-shell nanoparticles that provided films containing nanostructured polymers with much higher glass transition temperature (Tg) values...
October 4, 2016: Soft Matter
Tomohiro Seki, Keigo Abe, Yuya Egawa, Ryotaro Miki, Kazuhiko Juni, Toshinobu Seki
A pseudopolyrotaxane (PPRX) comprising 3-carboxy-5-nitrophrnylboronic acid-modified γ-cyclodextrin (NPBA-γ-CyD) and naphthalene-modified polyethylene glycol (Naph-PEG) as a sugar-responsive supramolecular structure is prepared. The binding of sugar by the NPBA group induced disintegration of the Naph-PEG/NPBA-γ-CyD PPRX, allowing the components to be dissolved. The Naph-PEG/NPBA-γ-CyD PPRX exhibited better sensitivity compared to that of a PPRX based on 4-carboxyphenylboronic acid-modified γ-cyclodextrin (PBA-γ-CyD)...
October 7, 2016: Molecular Pharmaceutics
Zheng-Nan Shan, Rui Tian, Min Zhang, Zhao-Hua Gui, Jing Wu, Min Ding, Xin-Fu Zhou, Jie He
MicroRNA128-1 (miR128-1), as a brain-specific miRNA, is downregulated in glioblastoma multiforme (GBM) and closely associated with the progression of GBM. However, the underlying molecular mechanism of the downregulation and its role in the regulation of tumorigenesis and anticancer drug resistance in GBM remains largely unknown. In the current study,we found that miR128-1 was downregulated in GBM and glioma stem-like cells (GSCs). Intriguingly, treatment with the DNA methylation inhibitors 5-Aza-CdR (Aza) and 4-phenylbutyric acid (PBA) resulted in miR128-1 upregulation in both GBM cells and GSCs...
October 1, 2016: Oncotarget
Michaela Matthes, Ramón A Torres-Ruiz
Several observations suggest that the micronutrient boron (B) has a stabilizing role in the plasma membrane (PM), supporting functions in PM-linked (hormone) signalling processes. However, this role is poorly characterized. This study shows, that boronic acids, specific competitors of B, induce phenocopies of the Arabidopsis thaliana rootless pattern mutant monopteros At least in part, this is caused by phenylboronic acid (PBA)-induced internalisation of the membrane-localized auxin efflux carrier PINFORMED1 (PIN1) in the early embryo...
October 3, 2016: Development
Qin Jiang, Ming Wang, Lifen Yang, Hui Chen, Lanqun Mao
Highly selective detection of intracellular glutamine (Gln) is very essential to understand the roles of Gln in some biological processes. Here, we report a new fluorescent method for selective imaging of Gln in live cells with an aldehyde-containing iridium complex, [Ir(pba)2(DMSO)2]PF6 (Hpba = 4-(2-pyridiyl)benzaldehyde) (Ir1), as the probe. Density functional theory (DFT) calculation and experimental results suggest that the coordination and hydrogen bonding interaction between Ir1 and Gln synergistically stabilize the Ir1-Gln complex, modulate charge-transfer characteristics and emission of Ir1, and as a consequence, enable Ir1 as the probe for the fluorescent sensing of Gln...
October 7, 2016: Analytical Chemistry
Jianhui Yang, Qiong Wu, Jianguo Lv, Huiyong Nie
Apoptosis of osteoblasts triggered by high-dose glucocorticoids (GCs) has been identified as a major cause of osteoporosis. However, the molecular mechanisms underlying GC-induced osteoporosis remain elusive. This study was conducted to make clear the mechanism of GC-induced osteoblast apoptosis and to examine whether reduction of ER stress by 4-PBA inhibited osteoblast apoptosis. After treatment with dexamethasone (Dex) or hydrocortisone, cell viability was assessed using an MTT assay. Flow cytometry was performed to assess the apoptosis of MC3T3-E1 cells...
September 27, 2016: Journal of Bone and Mineral Metabolism
Yuanting Zhu, Jianlong Li, Kai Yao, Nan Zhao, Kang Zhou, Xinjie Hu, Likou Zou, Xinfeng Han, Aiping Liu, Shuliang Liu
A novel filamentous fungus M-4 strain was isolated from soy sauce koji and identified as Aspergillus oryzae (Collection number: CGMCC 11645) on the basis of morphological characteristics and internal transcribed spacer sequence. M-4 could degrade 80.62 % of 3-phenoxybenzoic acid (3-PBA; 100 mg L(-1)) within 5 days. 3-PBA degradation occurred in accordance with first-order kinetics. The degradation metabolites of 3-PBA were identified through high-performance liquid chromatography-mass spectrometry (HPLC-MS)...
September 27, 2016: Applied Microbiology and Biotechnology
Jing Ma, Tao Luo, Zhi Zeng, Haiying Fu, Yoshihiro Asano, Yulin Liao, Tetsuo Minamino, Masafumi Kitakaze
4-Sodium phenylbutyrate (PBA) has been reported to inhibit endoplasmic reticulum stress and histone deacetylation (HDAC), both of which are novel therapeutic targets for cardiac hypertrophy and heart failure. However, it is unclear whether PBA can improve heart function. Here, we tested the effects of PBA and some other HDAC inhibitors on cardiac dysfunction induced by pressure overload. Transverse aortic constriction (TAC) was performed on male C57BL/6 mice. PBA treatment (100 mg/kg, 6 weeks) unexpectedly led to a higher mortality, exacerbated cardiac remodelling and dysfunction...
September 26, 2016: Scientific Reports
H Gao, X Y Zhang, L Y Yue, Z Y Liu, N N Jia, D X Chen, Z P Duan, F Ren
Objective: To study the role of endoplasmic reticulum (ER) stress-mediated glycogen synthase kinase 3β (GSK3β) activity in the pathological process of liver injury in acute liver failure (ALF) mice. Methods: ALF model was established by intraperitoneal injection of D-galactosamine/lipopolysaccharide (D-GalN/LPS) in C57BL/6 mice. The mice were divided into control group (n=10), ALF model group (n=18), 4-phenylbutyrate (4-PBA, an ER stress inhibitor) group (n=18) and SB216763 (a specific inhibitor of GSK3β) group (n=16)...
September 13, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Sabine Himmelein, Bart Jan Ravoo
A supramolecular carbohydrate sensor was prepared by self-assembly of unilamellar bilayer vesicles of amphiphilic β-cyclodextrin with a boronic acid-adamantane conjugate (PBA-AD), which binds strongly to β-cyclodextrin through host-guest interactions (Ka ≈4×10(4)  m(-1) ), so the vesicle surface exhibits multiple boronic acid receptors for carbohydrates. The binding of diols to the functionalized vesicles was tested with alizarin red S (ARS) as a reporter dye by using fluorescence and UV/Vis spectroscopy...
September 23, 2016: Chemistry: a European Journal
Meiling Zhou, Jianbo Li, Chunhong Li, Ling Guo, Xinyi Wang, Qin He, Yao Fu, Zhirong Zhang
In this work, two tertiary amine-derived 4'-demethylepipodophyllotoxin (DMEP) conjugates (DC and DP) have been designed and synthesized using N,N,N'-trimethyl-N'-(4-carboxyl benzyl)-1,3-propanediamine (CPDM) and 4-(4-methylpiperazinomethyl)benzoic acid (PBA) as the targeting ligands. Both DC and DP exhibited strong in vitro cytotoxicity against small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) cell lines. Cellular uptake efficiencies of DC and DP in human alveolar type II epithelial cells were significantly enhanced compared to DMEP and etoposide (VP-16), which were demonstrated to be concentration-, time- and energy-dependent...
November 10, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
Valérie Plaisance, Saška Brajkovic, Mathie Tenenbaum, Dimitri Favre, Hélène Ezanno, Amélie Bonnefond, Caroline Bonner, Valéry Gmyr, Julie Kerr-Conte, Benoit R Gauthier, Christian Widmann, Gérard Waeber, François Pattou, Philippe Froguel, Amar Abderrahmani
Elevated plasma concentration of the pro-atherogenic oxidized low density lipoprotein cholesterol (LDL) triggers adverse effects in pancreatic beta-cells and is associated with type 2 diabetes. Here, we investigated whether the endoplasmic reticulum (ER) stress is a key player coupling oxidative stress to beta-cell dysfunction and death elicited by human oxidized LDL. We found that human oxidized LDL activates ER stress as evidenced by the activation of the inositol requiring 1α, and the elevated expression of both DDIT3 (also called CHOP) and DNAJC3 (also called P58IPK) ER stress markers in isolated human islets and the mouse insulin secreting MIN6 cells...
2016: PloS One
Erica Miraglia, Frank Nylén, Katarina Johansson, Elias Arnér, Marcus Cebula, Susan Farmand, Håkan Ottosson, Roger Strömberg, Gudmundur H Gudmundsson, Birgitta Agerberth, Peter Bergman
Bacterial resistance against classical antibiotics is a growing problem and the development of new antibiotics is limited. Thus, novel alternatives to antibiotics are warranted. Antimicrobial peptides (AMPs) are effector molecules of innate immunity that can be induced by several compounds, including vitamin D and phenyl-butyrate (PBA). Utilizing a luciferase based assay, we recently discovered that the histone deacetylase inhibitor Entinostat is a potent inducer of the CAMP gene encoding the human cathelicidin LL-37...
2016: Scientific Reports
Tarun Keswani, Samrat Sarkar, Anirban Sengupta, Arindam Bhattacharyya
The role of cytokines in Plasmodium infection have been extensively investigated, but pro and anti inflammatory cytokines mediated imbalance during malaria immune-pathogenesis is still unrevealed. Malaria is associated with the circulating levels of Interleukin-6 (IL-6) and transforming growth factor β (TGF-β), but association between these two cytokines in immune response remains largely obscured. Using mouse model, we proposed that IL-6 and TGF-β are involved in immune regulation of dendritic cells (DC), regulatory T cells (Treg), T-helper cells (Th17) during P...
December 2016: Cytokine
Huanxian Chen, Linmin Chen, Liang Wang, Xinhua Zhou, Judy Yuet-Wa Chan, Jingjing Li, Guozhen Cui, Simon Ming-Yuen Lee
Curcumin and fenretinide are 2 well-known and promising chemotherapeutic compounds via various molecular mechanisms. However, the anticancer capacity of either curcumin or fenretinide is limited. This prompted us to examine the combined anticancer effects of curcumin and fenretinide. Our results demonstrate for the first time that there is synergistic anticancer effect of combined treatment with these 2 agents, leading to enhanced cytotoxicity and enhanced expression level of pro-apoptotic protein cleaved PARP in non-small cell lung cancer (NSCLC) cells while showed little toxicity to rat cardiomyoblast normal cells...
August 11, 2016: Cancer Biology & Therapy
Vasilios G Samaras, Anupam Giri, Zuzana Zelinkova, Lubomir Karasek, Gerhard Buttinger, Thomas Wenzl
Fatty acid esters of 3-monochloro-1,2-propanediol (3-MCPDEs), of 2-monochloro-1,3-propanediol (2-MCPDEs) and of 2,3-epoxy-1-propanol or glycidol (GEs), which are considered to be deleterious to human health, may occur in a broad variety of food samples. A proper risk assessment of those substances requires the availability of robust occurrence data; in this respect concerns have been raised regarding the reliability of results obtained with the currently available methods to determine those substances in processed food...
September 30, 2016: Journal of Chromatography. A
Hiromi Kusama, Kazuyoshi Kon, Kenichi Ikejima, Kumiko Arai, Tomonori Aoyama, Akira Uchiyama, Shunhei Yamashina, Sumio Watanabe
BACKGROUND: Acetaminophen (APAP) overdose induces severe oxidative stress followed by hepatocyte apoptosis/necrosis. Previous studies have indicated that endoplasmic reticulum (ER) stress is involved in the cell death process. Therefore, we investigated the effect of the chemical chaperone 4-phenyl butyric acid (PBA) on APAP-induced liver injury in mice. METHODS: Eight-week-old male C57Bl6/J mice were given a single intraperitoneal (i.p.) injection of APAP (450 mg/kg body weight), following which some were repeatedly injected with PBA (120 mg/kg body weight, i...
September 6, 2016: Journal of Gastroenterology
Sheng Chen, Shuo Gu, Yue Wang, Yongliang Yao, Guoquan Wang, Yue Jin, Yeming Wu
Pesticide exposure is hypothesized as one of the risk factors for the development of childhood brain tumors (CBT). This hospital-based case-control study evaluated the association of pyrethroid pesticide exposure with the risk for CBT in a children population in East China. In total, 161 CBT cases and 170 controls were recruited from 2 children's medical centers in Shanghai (Xinhua Hospital and Shanghai Children's Medical Center) between September 2012 and June 2015. The cases and controls were matched for age, sex, and province of residence...
November 2016: Environmental Pollution
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