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https://www.readbyqxmd.com/read/27911430/-clinical-presentations-of-herpes-zoster-ophthalmicus-diagnosis-and-therapy
#1
G M Chernakova, E A Kleshcheva, T B Semenova
: Approximately a quarter of the world's population at some point in life is at risk of developing shingles (Herpes Zoster). In 10-20% of cases the first branch of the trigeminal nerve gets involved (Herpes Zoster Ophthalmicus, HZO). Ophthalmic complications of HZO are able to cause a significant reduction in visual function. AIM: To study and summarize clinical features of HZO (including the rate of complications and their nature) and to determine the relationship between clinical and laboratory data from these patients...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27902903/characterization-of-thai-mycoplasma-synoviae-isolates-by-sequence-analysis-of-partial-vlha-gene
#2
Kriengwich Limpavithayakul, Jiroj Sasipreeyajan, Somsak Pakpinyo
Mycoplasma synoviae (MS), a remarkable pathogen in poultry, causes subclinical infection of the upper respiratory tract and an infectious synovitis, especially in the tendon sheaths and synovial membranes of joints. Because the specific detection of MS 16S rRNA gene-based PCR was unsuitable for strain differentiation, vlhA gene-based PCR was designed to differentiate the MS strains. The vlhA gene of MS encodes for hemagglutinin and other immunodominant membrane proteins involved in colonization, antigenic variations, and virulence...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27902896/the-efficacy-of-chitosan-adjuvanted-mycoplasma-gallisepticum-bacterin-in-chickens
#3
Arithat Limsatanun, Jiroj Sasipreeyajan, Somsak Pakpinyo
Mycoplasma gallisepticum (MG) is one of the major pathogens that cause respiratory signs in the poultry industry. To control MG infection, vaccination is the useful procedure. In this study, MG vaccine was developed by using the local Thai MG isolate (AHRL 20/52). Chitosan, a polysaccharide adjuvant derived from crustaceans, has been successfully used in various vaccines. The objectives of this study were to prepare MG bacterins by using chitosan, serving as an adjuvant, to determine protection against the field Thai MG isolate and to evaluate tissue reaction at the injection site...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27899204/stevens-johnson-syndrome-in-childhood
#4
N Blanco, B Gutiérrez, I Valls, D Puertas, C Martín, M Rivera, Á Hernández, A Torrelo
INTRODUCTION: Stevens-Johnson syndrome and toxic epidermal necrolysis are two serious immune diseases within the context of bullous mucocutaneous syndrome. These have varying degrees of involvement of the skin and usually at least two mucous membranes. CLINICAL CASES: Three clinical cases are presented, two of them with significant ophthalmological sequelae, who had received drug treatment as a possible trigger, and another milder clinical case caused by Mycoplasma pneumoniae...
November 26, 2016: Archivos de la Sociedad Española de Oftalmología
https://www.readbyqxmd.com/read/27898565/quantitative-real-time-polymerase-chain-reaction-for-the-diagnosis-of-mycoplasma-genitalium-infection-in-south-african-men-with-and-without-symptoms-of-urethritis
#5
Marie Cecilia le Roux, Anwar Ahmed Hoosen
BACKGROUND: This study was done to diagnose Mycoplasma genitalium infection based on bacterial load in urine specimens from symptomatic and asymptomatic men. METHODS: Urine specimens from 94 men with visible urethral discharge, 206 with burning on micturition and 75 without symptoms presenting to a family practitioner were tested for M. genitalium as well as Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis by transcription-mediated amplification assays...
November 28, 2016: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27897025/-nonculture-techniques-of-microorganisms-determination-in-amniotic-fluid-in-patients-with-preterm-prelabor-rupture-of-membranes
#6
R Kutová, L Plíšková, M Kacerovský
OBJECTIVE: The preterm prelabor rupture of membranes is a serious obstetric complication that is frequently complicated by the presence of microorganisms in amniotic fluid. The aim of our work is to characterize current status of nonculture detection of microbial invasion into the amniotic cavity and the experience with the technique performed in University Hospital in Hradec Kralove. DESIGN: Original survey article. SETTING: Institute of Clinical Biochemistry and Diagnostics - molecular biology department, University Hospital Hradec Kralove...
2016: Ceská Gynekologie
https://www.readbyqxmd.com/read/27895290/comparison-of-phenotypic-and-genotypic-profiles-among-caprine-and-ovine-mycoplasma-ovipneumoniae-strains
#7
Z Maksimović, C De la Fe, J Amores, Á Gómez-Martín, M Rifatbegović
Mycoplasma ovipneumoniae (Movp) is considered to be one of the most important mycoplasmas causing respiratory disease in small ruminants. Most epidemiologic and characterisation studies have been conducted on strains collected from sheep. Information on the presence and characteristics of Movp in healthy and pneumonic goats is limited. Phenotypic or genotypic differences between sheep and goat isolates have never been studied. The objective of our study was to characterise and compare the similarities and differences between caprine and ovine Movp strains isolated from affected and asymptomatic animals in order to elucidate phenotypic and genotypic variability...
November 28, 2016: Veterinary Record
https://www.readbyqxmd.com/read/27895010/mutations-associated-with-decreased-susceptibility-to-seven-antimicrobial-families-in-field-and-laboratory-derived-mycoplasma-bovis-strains
#8
Kinga M Sulyok, Zsuzsa Kreizinger, Enikő Wehmann, Inna Lysnyansky, Krisztián Bányai, Szilvia Marton, Ákos Jerzsele, Zsuzsanna Rónai, Ibolya Turcsányi, László Makrai, Szilárd Jánosi, Sára Ágnes Nagy, Miklós Gyuranecz
The molecular mechanisms of resistance to fluoroquinolones, tetracyclines, an aminocyclitol, macrolides, a lincosamide, a phenicol and pleuromutilins were investigated in Mycoplasma bovis For the identification of mutations responsible for the high minimal inhibitory concentrations (MICs) to certain antibiotics, whole genome sequencing of 35 M. bovis field isolates and 36 laboratory-derived antibiotic resistant mutants was performed. In vitro resistant mutants were selected by serial passages of M. bovis in broth medium containing subinhibitory concentrations of the antibiotics...
November 28, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27894204/clinical-laboratory-assessments-for-mycoplasma-genitalium-in-a-high-prevalence-sexually-transmitted-infection-community-reveal-epidemiologic-dichotomies-with-trichomonas-vaginalis
#9
Erik Munson, Kimber L Munson, Ronald F Schell
Mycoplasma genitalium is an emerging agent of sexually-transmitted infection and is responsible for clinically-significant genital tract disease in both females and males. Similar to scenarios recently experienced with the urogenital flagellate Trichomonas vaginalis, an evolving molecular diagnostic reference standard based on transcription-mediated amplification allows for accurate detection of the organism, plus additional insight into disease epidemiology. Areas covered. The basis for this article includes primary peer-reviewed literature plus compilations of data derived from routine clinical laboratory screening of females and males for agents of sexually-transmitted infection...
November 29, 2016: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/27893691/valuable-hematological-indicators-for-the-diagnosis-and-severity-assessment-of-chinese-children-with-community-acquired-pneumonia-prealbumin
#10
Jingjing Ning, Xiaonan Shao, Yibo Ma, Darong Lv
Chest X-ray is a "golden standard" for the diagnosis and severity assessment of community-acquired pneumonia (CAP). However, it cannot be used as routine examination of CAP in children. The present study aims to investigate the roles of prealbumin (PA) in CAP in children and further determine the usefulness of PA in diagnosis and severity assessment of CAP in children.This was a retrospective analysis of 174 cases of hospitalized children with CAP. The following indicators were recorded: vital sign, inflammatory indexes, PA, and respiratory pathogens immunoglobulin M antibody test results...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27893600/prevalence-and-correlates-of-genital-infections-among-newly-diagnosed-human-immunodeficiency-virus-infected-adults-entering-human-immunodeficiency-virus-care-in-windhoek-namibia
#11
Gaston Djomand, Madeleine Schlefer, Steve Gutreuter, Sarah Tobias, Roopal Patel, Nickolas DeLuca, Julia Hood, Souleymane Sawadogo, Cheng Chen, Alexinah Muadinohamba, David W Lowrance, Naomi Bock
BACKGROUND: Identifying and treating genital infections, including sexually transmitted infections (STI), among newly diagnosed human immunodeficiency virus (HIV)-infected individuals may benefit both public and individual health. We assessed prevalence of genital infections and their correlates among newly diagnosed HIV-infected individuals enrolling in HIV care services in Namibia. METHODS: Newly diagnosed HIV-infected adults entering HIV care at 2 health facilities in Windhoek, Namibia, were recruited from December 2012 to March 2014...
November 2016: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27884651/analysis-of-mutations-in-dna-gyrase-and-topoisomerase-iv-of-ureaplasma-urealyticum-and-ureaplasma-parvum-serovars-resistant-to-fluoroquinolones
#12
Giorgio Piccinelli, Franco Gargiulo, Valeria Biscaro, Francesca Caccuri, Arnaldo Caruso, Maria Antonia De Francesco
This study aims to determine the prevalence of fluoroquinolone resistance of Ureaplasma biovars and serovars isolated from urogenital clinical samples and determine the underlying molecular mechanism for quinolone resistance for all resistant isolates. Of 105 samples confirmed as positive for U. urealyticum/U. parvum, 85 were resistant to quinolones by the Mycoplasma-IST2 kit. However, only 43 out of 85 quinolone resistant isolates had amino acid substitutions in GyrA, GyrB, ParC and ParE proteins underlining that this assay have mis-identified as fluoroquinolone resistant 42 isolates...
November 21, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27884082/enhanced-conformational-flexibility-of-the-histone-like-hu-protein-from-mycoplasma-gallisepticum
#13
Dmitry A Altukhov, Anna A Talyzina, Yulia K Agapova, Anna V Vlaskina, Dmitry A Korzhenevskiy, Eduard V Bocharov, Tatiana V Rakitina, Vladimir I Timofeev, Vladimir O Popov
The histone-like (HU) protein is one of the major nucleoid-associated proteins involved in DNA supercoiling and compaction into bacterial nucleoid as well as in all DNA-dependent transactions. This small positively charged dimeric protein binds DNA in a non-sequence specific manner promoting DNA super-structures. The majority of HU proteins are highly conserved among bacteria; however, HU protein from Mycoplasma gallisepticum (HUMgal) has multiple amino acid substitutions in the most conserved regions, which are believed to contribute to its specificity to DNA targets unusual for canonical HU proteins...
November 24, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27882384/prevalence-of-mycoplasma-genitalium-infection-and-relationship-with-symptoms-among-adults-attending-a-sexual-health-centre
#14
Maeva Lefebvre, Julie Coutherut, Sophie Gibaud, Charlotte Biron, Marine Chalopin, Claire Bernier, François Raffi
is missing (Short communication).
November 24, 2016: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/27881098/genomic-determination-of-minimum-multi-locus-sequence-typing-schemas-to-represent-the-genomic-phylogeny-of-mycoplasma-hominis
#15
Aleksey Jironkin, Rebecca J Brown, Anthony Underwood, Victoria J Chalker, Owen B Spiller
BACKGROUND: Mycoplasma hominis is an opportunistic human pathogen, associated with clinically diverse disease. Currently, there is no standardised method for typing M. hominis, which would aid in understanding pathogen epidemiology and transmission. Due to availability and costs of whole genome sequencing and the challenges in obtaining adequate M. hominis DNA, the use of whole genome sequence analysis to provide clinical guidance is unpractical for this bacterial species as well as other fastidious organisms...
November 23, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27875955/detection-the-mycoplasma-pneumonia-in-synovial-fluid-of-children-with-negative-culture-arthritis-a-cross-sectional-study-in-tehran-iran
#16
Samileh Noorbakhsh, Mohammad Reza Shokrollahi, Farideh Ebrahimi Taj, Vida Zarabi, Azardokht Tabatabaei
BACKGROUND: arthritis could be caused by different etiologies ranging from rheumatologic diseases to infectious conditions. Therefore, early diagnosis of etiology and treatment is important. The purpose of this study was to determine the the M.pneumonia in synovial fluid of children with arthritis by 2 methods (serology and qualitative PCR). Methods & materials: this trial was carried out as a cross sectional study in pediatric and orthopedic ward of Rasoul-e Akram hospital in Tehran, Iran...
November 21, 2016: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/27872715/molecular-characterization-of-mycoplasma-synoviae-isolated-from-broiler-chickens-of-west-azarbaijan-province-by-pcr-of-vlha-gene
#17
Abolfazl Ghaniei
Mycoplasma synoviae (MS) is a pathogen responsible for respiratory and locomotor disorders and causes major economic losses in poultry industry. Early and accurate diagnosis of MS infection plays a major role in control of the infection. This study was conducted to characterize Iranian field isolates of MS isolated from broiler chickens of West Azarbaijan province (Northwest of Iran), and differentiate them from vaccine strain MS-H. Two encoding genes, 16S rRNA and vlhA were employed. PCR results using primers related to 16s rRNA and vlhA genes were analyzed and compared...
2016: Veterinary Research Forum
https://www.readbyqxmd.com/read/27872239/experimental-infection-of-pig-tailed-macaques-macaca-nemestrina-with-mycoplasma-genitalium
#18
Gwendolyn E Wood, Dorothy L Patton, Peter K Cummings, Stefanie L Iverson-Cabral, Patricia A Totten
Mycoplasma genitalium is an underappreciated cause of human reproductive tract disease, characterized by persistent, often asymptomatic, infection. Building on our previous experiments using a single female pig-tailed macaque as a model for M. genitalium infection (G. E. Wood, S. L. Iverson-Cabral, D. L. Patton, P. K. Cummings, Y. T. Cosgrove Sweeney, and P. A. Totten, Infect Immun 81:2938-2951, 2013, doi: 10.1128/IAI.01322-12), we cervically inoculated eight additional animals, two of which were simultaneously inoculated in salpingeal tissue autotransplanted into abdominal pockets...
November 21, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27871827/asymptomatic-infection-with-mycoplasma-hominis-negatively-affects-semen-parameters-and-leads-to-male-infertility-as-confirmed-by-improved-semen-parameters-after-antibiotic-treatment
#19
Mohammad Hossein Ahmadi, Akbar Mirsalehian, Mohammad Ali Sadighi Gilani, Abbas Bahador, Malihe Talebi
OBJECTIVES: To elucidate the association between asymptomatic infections caused by Mycoplasma hominis and male infertility and evaluate the role of antibiotic therapy in treatment of this failure. METHODS: A total of 165 infertile males having abnormal semen parameters (study group) as well as 165 healthy fertile men (control group) were included in this study. Semen samples were taken from all participants and after analyzing for semen parameters, undergone real-time PCR, microbial culture, and reactive oxygen species (ROS) as well as total antioxidant capacity (TAC) assays...
November 18, 2016: Urology
https://www.readbyqxmd.com/read/27871125/eye-of-the-finch-characterization-of-the-ocular-microbiome-of-house-finches-in-relation-to-mycoplasmal-conjunctivitis
#20
Courtney A Thomason, Ariel Leon, Laila T Kirkpatrick, Lisa K Belden, Dana M Hawley
Vertebrate ocular microbiomes are poorly characterized and virtually unexplored in wildlife species. Pathogen defense is considered a key function of microbiomes, but determining microbiome stability during disease is critical for understanding the role of resident microbial communities in infectious disease dynamics. Here, we characterize the ocular bacterial microbiome of house finches (Haemorhous mexicanus), prior to and during experimental infection with an inflammatory ocular disease, Mycoplasmal conjunctivitis, caused by Mycoplasma gallisepticum...
November 21, 2016: Environmental Microbiology
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