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https://www.readbyqxmd.com/read/28930847/guillain-barr%C3%A3-like-axonal-polyneuropathy-associated-with-toscana-virus-infection-a-case-report
#1
Eugenia Rota, Nicola Morelli, Paolo Immovilli, Paola De Mitri, Donata Guidetti
RATIONALE: Numerous cases of post-infectious Guillain-Barré syndrome (GBS) have been reported in the literature. Toscana virus (TOSV) is an arthropod-borne emerging pathogen in the Mediterranean area. PATIENT CONCERNS: A 40-year-old male patient was admitted to hospital for acute facial weakness, associated to numbness paraesthesias at lower and upper limbs. The neurological examination revealed facial diplegia and reduced tendon reflexes. The nerve conduction studies documented an acute motor and sensory axonal neuropathy (AMSAN); the lumbar puncture detected albuminocytologic dissociation...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28930026/macrolide-resistant-mycoplasma-pneumoniae-infection-japan-2008-2015
#2
Takaaki Tanaka, Tomohiro Oishi, Ippei Miyata, Shoko Wakabayashi, Mina Kono, Sahoko Ono, Atsushi Kato, Yoko Fukuda, Aki Saito, Eisuke Kondo, Hideto Teranishi, Yuhei Tanaka, Tokio Wakabayashi, Hiroto Akaike, Satoko Ogita, Naoki Ohno, Takashi Nakano, Kihei Terada, Kazunobu Ouchi
We evaluated isolates obtained from children with Mycoplasma pneumoniae infection throughout Japan during 2008-2015. The highest prevalence of macrolide-resistant M. pneumoniae was 81.6% in 2012, followed by 59.3% in 2014 and 43.6% in 2015. The prevalence of macrolide-resistant M. pneumoniae among children in Japan has decreased.
October 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28927691/comparative-genomics-of-four-mycoplasma-species-of-the-human-urogenital-tract-analysis-of-their-core-genomes-and-virulence-genes
#3
Orville St E Roachford, Karen E Nelson, Bidyut R Mohapatra
The variation in Mycoplasma lipoproteins attributed to genome rearrangements and genetic insertions leads to phenotypic plasticity that allows for the evasion of the host's defence system and pathogenesis. This paper compared for the first time the genomes of four human urogenital Mycoplasma species (M. penetrans HF-2, M. fermentans JER, M. genitalium G37 and M. hominis PG21) to categorise the metabolic functions of the core genes and to assess the effects of tandem repeats, phage-like genetic elements and prophages on the virulence genes...
September 5, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28927255/innate-immune-response-of-bovine-mammary-epithelial-cells-to-mycoplasma-bovis
#4
Satoshi Gondaira, Hidetoshi Higuchi, Hidetomo Iwano, Koji Nishi, Takanori Nebu, Keiichi Nakajima, Hajime Nagahata
Mycoplasma species are contagious bacteria and Mycoplasma bovis mastitis is a serious productivity problem on dairy farms. Bovine mammary epithelial cells (bMEC) play an important role in the elimination of pathogens, but the effect of M. bovis on bMEC has not been fully clarified. To better understand the immune response against intramammary infection by M. bovis, we used microarray analysis to examine and profile mRNA expression in bMEC after stimulation with M. bovis. We also compared the effects of M. bovis, Staphylococcus aureus and Escherichia coli on immune-related mRNA expression in bMEC...
September 20, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28923377/clinical-and-analytical-evaluation-of-the-new-aptima-mycoplasma-genitalium-assay-with-data-on-m-genitalium-prevalence-and-antimicrobial-resistance-in-m-genitalium-in-denmark-norway-and-sweden-in-2016
#5
M Unemo, K Salado-Rasmussen, M Hansen, A O Olsen, M Falk, D Golparian, M Aasterød, J Ringlander, C Steczkó Nilsson, M Sundqvist, K Schønning, H Moi, H Westh, J S Jensen
OBJECTIVES: Mycoplasma genitalium (MG) causes urethritis and cervicitis, potentially causing reproductive complications. Resistance in MG to first-line (azithromycin) and second-line (moxifloxacin) treatment has increased. We examined the clinical and analytical performance of the new CE/IVD Aptima Mycoplasma genitalium assay (CE/IVD AMG; Hologic); the prevalence of MG, Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG); and MG resistance to azithromycin and moxifloxacin in Denmark, Norway and Sweden in 2016...
September 15, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28922613/the-etiology-of-community-acquired-pneumonia-among-children-under-5%C3%A2-years-of-age-in-mainland-china-2001-2015-a-systematic-review
#6
Guijun Ning, Xuxia Wang, Dan Wu, Zundong Yin, Yixing Li, Huaqing Wang, Weizhong Yang
BACKGROUND: The aim of this systematic review was to examine the etiology of community-acquired pneumonia (CAP) among Chinese children younger than 5 years and provide evidence for further cost-effectiveness analyses for vaccine development, diagnostic strategies and empirical treatments. METHODS: The literature review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement. Data were obtained by searching PubMed, Embase, Web-of Science, and the Chinese databases Wanfang Data and China National Knowledge Infrastructure...
September 18, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28920779/ocular-findings-and-select-ophthalmic-diagnostic-tests-in-captive-american-white-pelicans-pelecanus-erythrorhynchos
#7
Matthew E Kinney, Aaron C Ericsson, Craig L Franklin, Rebecca E H Whiting, Jacqueline W Pearce
The aim of this study was to establish normal ophthalmic parameters for select diagnostic tests in American white pelicans (Pelecanuserythrorhynchos). Twenty-one zoo-housed American white pelicans were manually restrained for noninvasive ocular diagnostic testing and complete ophthalmic examination. Tear production quantification using the phenol red thread test (PRTT), fluorescein staining, and intraocular pressure (IOP) evaluation were performed. In addition, conjunctival aerobic bacterial culture and culture-independent 16S rRNA amplicon sequencing were performed on select eyes...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28919684/molecular-screening-for-hemotropic-mycoplasmas-in-captive-barbary-sheep-ammotragus-lervia-in-southern-brazil
#8
Leonilda C Santos, Odilon Vidotto, Vivien M Morikawa, Nelson J R Santos, Thállitha S W J Vieira, Ivan R Barros Filho, Rafael F C Vieira, Alexander W Biondo
AIM: This study is part of an active surveillance program for monitoring animal health status in endangered species, and was conducted to screen captive Barbary sheep (Ammotragus lervia) for hemoplasma infection. MATERIALS AND METHODS: A total of 12 blood samples were collected, DNA extracted and further tested by a pan-hemoplasma polymerase chain reaction protocol. RESULTS: Animals were clinically healthy and not infested by ectoparasites...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28915995/detection-and-molecular-characterization-of-feline-hemoplasmas-in-wild-felid-species-in-iran-in-the-middle-east
#9
Fereshteh Ghazisaeedi, Nahid Atyabi, Taghi Zahraei Salehi, Saeid Tabatabaei, Iraj Ashrafi Tamai, Iman Memarian, Séverine Tasker
Three feline hemoplasma species exist in felids: Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum', and 'Candidatus Mycoplasma turicensis'. The aims of the study were to determine the presence of, and molecularly characterize, any hemoplasmas in wild felids, including the endangered Persian leopard in Iran, the Middle East. Blood samples were collected from 19 wild felids, including three Persian leopards. Using species-specific hemoplasma PCRs and ELISA serological testing for feline leukaemia virus and feline immunodeficiency virus (FIV), two Persian leopards were found to be infected with 'Ca...
October 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28915878/the-immune-mechanism-of-mycoplasma-hyopneumoniae-168-vaccine-strain-through-dendritic-cells
#10
Yumeng Shen, Weiwei Hu, Yanna Wei, Zhixin Feng, Qian Yang
BACKGROUND: Mycoplasma hyopneumoniae (Mhp) causes porcine enzootic pneumonia, a disease that cause major economic losses in the pig industry. Dendritic cells (DCs), the most effective antigen-presenting cells, are widely distributed beneath respiratory epithelium, DCs uptake and present antigens to T cells, to initiate protective immune responses in different infections. In this study, we investigated the role of porcine DCs in vaccine Mhp-168 exposure. RESULTS: The antigen presenting ability of DCs were improved by vaccine Mhp-168 exposure...
September 15, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28915064/the-immunopathologic-effects-of-mycoplasma-pneumoniae-and-cards-toxin-a-primate-model
#11
Diego J Maselli, Jorge L Medina, Edward G Brooks, Jacqueline J Coalson, Thirumalai R Kannan, Vicki T Winter, Molly Principe, Marianna P Cagle, Joel B Baseman, Peter H Dube, Jay I Peters
Mycoplasma pneumoniae infection has been linked to poor asthma outcomes. M. pneumoniae produces an ADP-ribosylating and vacuolating toxin called Community Acquired Respiratory Distress Syndrome (CARDS) toxin that has a major role in inflammation and airway dysfunction. The objective was to evaluate the immunopathological effects in primates exposed to M. pneumoniae or CARDS toxin. Thirteen baboons were exposed to M. pneumoniae or CARDS toxin. At day 7 and 14, bronchoalveolar lavage fluid was collected and analyzed for cell count, percent of each type of cell, CARDS toxin by PCR, CARDS toxin by antigen capture, eosinophilic cationic protein, and cytokine profiles...
September 15, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28912822/a-rare-case-of-cavitary-lesion-of-the-lung-caused-by-mycoplasma-pneumoniae-in-an-immunocompetent-patient
#12
Muhammad Kashif, Rizwan Ahmed Dudekula, Misbahuddin Khaja
Mycoplasma pneumoniae is an atypical bacterium that most commonly causes upper respiratory tract infections, but it can also cause pneumonia, referred to as "walking pneumonia." Although cavitary lesions are present in a wide variety of infectious and noninfectious processes, those attributable to M. pneumoniae are extremely uncommon; thus, to date, epidemiological studies are lacking. Here, we present a rare case of a 20-year-old male, referred to us from a psychiatric facility for evaluation of a cough, who was found to have a cavitary lesion in the right upper lobe...
2017: Case Reports in Medicine
https://www.readbyqxmd.com/read/28906180/amplification-of-mycoplasma-haemofelis-dna-by-a-pcr-for-point-of-care-use
#13
Jennifer Hawley, Tal Yaaran, Sarah Maurice, Michael R Lappin
We compared a qualitative in-clinic (IC)-PCR for the detection of Mycoplasma haemofelis DNA with the results of a commercial qualitative laboratory-based, conventional (c)PCR. In order to determine the specificity of both tests, Bartonella spp. samples were included. Forty-three previously tested blood samples with known PCR results for hemoplasmas and Bartonella spp. were selected. The samples were split between 2 laboratories. At the first laboratory, DNA was purified and run on 2 cPCR assays for the detection of hemoplasmas and Bartonella spp...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28906179/multi-locus-sequence-types-of-mycoplasma-bovis-isolated-from-ontario-canada-in-the-past-three-decades-have-a-temporal-distribution
#14
Patricia Bell-Rogers, Lois Parker, Hugh Y Cai
A total of 217 Mycoplasma bovis isolates cultured from clinical cases in Ontario, Canada, over the past 30 y were selected to be characterized by a multi-locus sequence typing (MLST) method. Eleven housekeeping genes were evaluated for suitability for MLST; 2 loci that had been used in prior MLST schemes, dnaN and metS, along with hsp70 were chosen for further sequence analysis. The remaining loci- adk, efp, gmk, gyrB, polC, rpoB, tpiA, and uvrC genes-were not used because they had little to no sequence variation...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28905982/vector-borne-diseases-in-cats-in-germany
#15
Michèle Bergmann, Katrin Hartmann
Vector-borne diseases (VBDs) are caused by a wide range of pathogens, which are transmitted by a variety of vectors, such as ticks and fleas. As a result of climate changes, more vector-borne diseases are becoming endemic in Germany, not only in dogs, but also in cats. For some of the pathogens prevalence data still need to be investigated in Germany. However, natural infections with Bartonella, Anaplasma, haemotropic Mycoplasma and Borrelia species have already been described in German cats. Clinical relevance of these pathogens is not fully understood, and it is still unknown, why most infected cats stay asymptomatic and which predisposing factors contribute to the development of clinical signs in cats...
September 13, 2017: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
https://www.readbyqxmd.com/read/28905531/elucidation-of-bacterial-pneumonia-causing-pathogens-in-patients-with-respiratory-viral-infection
#16
Hwa Sik Jung, Byung Ju Kang, Seung Won Ra, Kwang Won Seo, Yangjin Jegal, Jae Bum Jun, Jiwon Jung, Joseph Jeong, Hee Jeong Jeon, Jae Sung Ahn, Taehoon Lee, Jong Joon Ahn
BACKGROUND: Bacterial pneumonia occurring after respiratory viral infection is common. However, the predominant bacterial species causing pneumonia secondary to respiratory viral infections other than influenza remain unknown. The purpose of this study was to know whether the pathogens causing post-viral bacterial pneumonia vary according to the type of respiratory virus. METHODS: Study subjects were 5,298 patients, who underwent multiplex real-time polymerase chain reaction for simultaneous detection of respiratory viruses, among who visited the emergency department or outpatient clinic with respiratory symptoms at Ulsan University Hospital between April 2013 and March 2016...
September 1, 2017: Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28903876/persistence-of-mycoplasma-hyopneumoniae-sequence-types-in-spite-of-a-control-program-for-enzootic-pneumonia-in-pigs
#17
Gudrun Overesch, Peter Kuhnert
Enzootic pneumonia (EP) in pigs caused by Mycoplasma (M.) hyopneumoniae has successfully been combatted in Switzerland. A control program was fully implemented in 2004 which is based on total depopulation strategies of affected fattening farms as well as partial depopulation on breeding farms. Thereby, the number of cases has dropped drastically from more than 200 in 2003 to two cases in 2013. Currently monitoring is done based on clinical observation and subsequent diagnostic of coughing pigs. Moreover, in case of more than 10% gross pathological lesions per slaughter batch laboratory confirmation for EP is compulsory...
September 15, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28902958/nonregenerative-immune-mediated-anemia-associated-with-a-diffuse-large-b-cell-lymphoma-in-a-captive-jaguar-panthera-onca
#18
Monika A Keresztes, Manfred Henrich, Penelope Baloi, Sascha Gerst, Jens-Christian Rudnick, Judith Langenstein, Andreas Moritz, Natali Bauer
An 18-year-old male castrated jaguar (Panthera onca) was presented with anorexia and continuous bleeding from the oral cavity after a history of fighting with the partner animal. Clinical evaluation revealed ulcerating lesions on the gingiva and hard palate and a hematoma on the tongue. Computed tomography of the head and endoscopic examination of the esophagus and stomach were unremarkable. Hematology and clinical chemistry revealed severe nonregenerative anemia, mild thrombocytopenia, and moderate azotemia...
September 13, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28901843/coevolution-of-the-translational-machinery-optimizes-initiation-with-unusual-initiator-trnas-and-initiation-codons-in-mycoplasmas
#19
Shreya Ahana Ayyub, Divya Dobriyal, Riyaz Ahmad Shah, Kuldeep Lahry, Madhumita Bhattacharyya, Souvik Bhattacharyya, Saikat Chakrabarti, Umesh Varshney
Initiator tRNAs (i-tRNAs) are characterized by the presence of three consecutive GC base pairs (GC/GC/GC) in their anticodon stems in all domains of life. However, many mycoplasmas possess unconventional i-tRNAs wherein the highly conserved sequence of GC/GC/GC is represented by AU/GC/GC, GC/GC/GU or AU/GC/GU. These mycoplasmas also tend to preferentially utilize non-AUG initiation codons. To investigate if initiation with the unconventional i-tRNAs and non-AUG codons in mycoplasmas correlated with the changes in the other components of the translation machinery, we carried out multiple sequence alignments of genes encoding initiation factors (IF), 16S rRNAs, and the ribosomal proteins such as uS9, uS12 and uS13...
September 13, 2017: RNA Biology
https://www.readbyqxmd.com/read/28900116/response-induced-in-mycoplasma-gallisepticum-under-heat-shock-might-be-relevant-to-infection-process
#20
Ivan Butenko, Anna Vanyushkina, Olga Pobeguts, Daria Matyushkina, Sergey Kovalchuk, Alexey Gorbachev, Nicolay Anikanov, Gleb Fisunov, Vadim Govorun
Despite the fact the term "proteome" was proposed to characterize a set of proteins in one of mycoplasma species, proteome response to various exposures in this bacteria are still obscure. Commonly, authors studying proteomic response on perturbation models in mycoplasmas use single approach and do not confirm their findings by alternative methods. Consequently, the results of proteomic analysis should be validated by complementary techniques. In this study we utilized three complementary approaches (SWATH, MRM, 2D-DIGE) to assess response of Mycoplasma gallisepticum under heat stress on proteomic level and combined these findings with metabolic response and the results of transcriptional profiling...
September 12, 2017: Scientific Reports
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