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https://www.readbyqxmd.com/read/28813455/maternal-serum-c-reactive-protein-concentration-and-intra-amniotic-inflammation-in-women-with-preterm-prelabor-rupture-of-membranes
#1
Ivana Musilova, Marian Kacerovsky, Martin Stepan, Tomas Bestvina, Lenka Pliskova, Barbora Zednikova, Bo Jacobsson
OBJECTIVE: To evaluate maternal serum C-reactive protein (CRP) concentrations in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) in relation to the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). METHODS: Two hundred and eighty-seven women with singleton pregnancies complicated by PPROM between 2014 and 2016 were included in this study. Maternal blood and amniotic fluid samples were collected at the time of admission...
2017: PloS One
https://www.readbyqxmd.com/read/28811610/antibiotic-induced-release-of-small-extracellular-vesicles-exosomes-with-surface-associated-dna
#2
Andrea Németh, Norbert Orgovan, Barbara W Sódar, Xabier Osteikoetxea, Krisztina Pálóczi, Katalin É Szabó-Taylor, Krisztina V Vukman, Ágnes Kittel, Lilla Turiák, Zoltán Wiener, Sára Tóth, László Drahos, Károly Vékey, Robert Horvath, Edit I Buzás
Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against Mycoplasma contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811193/a-novel-detection-procedure-for-mutations-in-the-23s-rrna-gene-of-mycoplasma-pneumoniae-with-peptide-nucleic-acid-mediated-loop-mediated-isothermal-amplification-assay
#3
Jun Sakai, Takuya Maeda, Norihito Tarumoto, Kazuhisa Misawa, Shinsuke Tamura, Kazuo Imai, Toshiyuki Yamaguchi, Satoshi Iwata, Takashi Murakami, Shigefumi Maesaki
Rapid and easy detection of a single nucleotide point mutation of bacterial genes, which is directly linked to drug susceptibility, is essential for the proper use of antimicrobial agents. Here, we established a detection method using a peptide nucleic acid mediated loop-mediated amplification (LAMP) assay for macrolide (ML)-susceptible Mycoplasma pneumoniae. This assay specifically detected the absence of missense mutations encoding the central loop of domain V in the gene encoding 23S rRNA, which can reduce the affinity for MLs and subsequently generate ML-resistant strains of M...
August 12, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28810870/vector-borne-and-zoonotic-diseases-of-dogs-in-north-west-new-south-wales-and-the-northern-territory-australia
#4
Amanda J Shapiro, Graeme Brown, Jacqueline M Norris, Katrina L Bosward, Debbie J Marriot, Nandhakumar Balakrishnan, Edward B Breitschwerdt, Richard Malik
BACKGROUND: Vector-borne diseases of dogs in Australian Aboriginal communities are relatively unexplored. These dogs represent a unique group with variable ecto- and endo-parasitic burdens, nutritional stresses and a general lack of veterinary intervention. We investigated haemoprotozoal and bacterial pathogen prevalences in relation to erythrocyte and platelet numbers in dogs from North-West New South Wales (N-W NSW) and the Northern Territory (NT; Central Australia). METHODS: Real-time PCR (qPCR) amplification of Anaplasma platys, Babesia vogeli, Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum and Bartonella spp...
August 15, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28810609/value-of-serum-mycoplasma-pneumoniae-immunoglobulin-in-the-diagnosis-of-mycoplasma-related-pneumonia-in-newborns
#5
Yan Dong, Wei Lv, Zhen Lin
Mycoplasma pneumoniae (M. pneumoniae) is an important pathogen of neonatal acquired pneumonia in newborns. Rapid and accurate diagnosis of M. pneumoniae infection is critical because timely antibiotic therapy can reduce drug overuse and prevent the development of bacterial resistance. Anti-M. pneumoniae immunoglobulin M (IgM) is an indicator of early infection that can persist for several months. Studies have shown that anti-M. pneumoniae IgA in adults is a reliable indicator of early M. pneumoniae infection...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28807761/mycoplasma-hyopneumoniae-detection-in-nylon-flocked-and-rayon-bud-swabs
#6
Karine L Takeuti, David E S N de Barcellos, Maria Pieters
The objective of this study was to compare the absorption and M. hyopneumoniae detection by real-time PCR using nylon-flocked and rayon-bud swabs. Results of this study showed that absorption and detection of M. hyopneumoniae were significantly higher in nylon-flocked swabs with Ct differences only ranging from 0.5 to 1.7.
August 11, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28807289/neglected-intravascular-pathogens-babesia-vulpes-and-haemotropic-mycoplasma-spp-in-european-red-fox-vulpes-vulpes-population
#7
Martina Koneval, Martina Miterpáková, Zuzana Hurníková, Lucia Blaňarová, Bronislava Víchová
Wild animals, especially canids, are important reservoirs of vector-borne pathogens, that are transmitted by the ticks and other bloodsucking arthropods. In total, 300 red foxes (Vulpes vulpes), shot by the hunters in eastern and northern Slovakia, were screened for the presence of vector-borne pathogens by PCR-based methods Blood samples were obtained from nine red foxes and tissue samples originated from 291 animals (the liver tissue samples from 49 foxes and spleen samples from 242 red foxes). Babesia vulpes and haemotropic Mycoplasma species were identified by amplification and sequencing of 18S rRNA and 16S rRNA gene fragments, respectively...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28807095/simultaneous-detection-of-13-key-bacterial-respiratory-pathogens-by-combination-of-multiplex-pcr-and-capillary-electrophoresis
#8
Lu Xi Jiang, Hong Yu Ren, Hai Jian Zhou, Si Hong Zhao, Bo Yan Hou, Jian Ping Yan, Tian Qin, Yu Chen
OBJECTIVE: Lower respiratory tract infections continue to pose a significant threat to human health. It is important to accurately and rapidly detect respiratory bacteria. To compensate for the limits of current respiratory bacteria detection methods, we developed a combination of multiplex polymerase chain reaction (PCR) and capillary electrophoresis (MPCE) assay to detect thirteen bacterial pathogens responsible for lower respiratory tract infections, including Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Mycoplasma pneumoniae, Legionella spp...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28806998/first-report-of-sneathia-sanguinegens-together-with-mycoplasma-hominis-in-postpartum-prosthetic-valve-infective-endocarditis-a-case-report
#9
Iva Kotaskova, Petr Nemec, Martina Vanerkova, Barbora Malisova, Renata Tejkalova, Marek Orban, Vita Zampachova, Tomas Freiberger
BACKGROUND: The presence of more than one bacterial agent is relatively rare in infective endocarditis, although more common in prosthetic cases. Molecular diagnosis from a removed heart tissue is considered a quick and effective way to diagnose fastidious or intracellular agents. CASE PRESENTATION: Here we describe the case of postpartum polymicrobial prosthetic valve endocarditis in a young woman. Sneathia sanguinegens and Mycoplasma hominis were simultaneously detected from the heart valve sample using broad range 16S rRNA polymerase chain reaction (PCR) followed by sequencing while culture remained negative...
August 14, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28803864/the-detection-of-microorganisms-related-to-urethritis-from-the-oral-cavity-of-male-patients-with-urethritis
#10
Phuong Thi Le, Ryoichi Hamasuna, Masahiro Matsumoto, Keiichi Furubayashi, Masayuki Hatanaka, Shuichi Kawai, Takamasa Yamaguchi, Kazutaka Uehara, Norihiko Murakami, Masaru Yoshioka, Ken Nakayama, Yutaka Shiono, Keisuke Muraoka, Masahiko Suzuki, Naohiro Fujimoto, Tetsuro Matsumoto
OBJECTIVES: To investigate the presence of microorganisms related to urethritis in the oral cavity of male patients with urethritis and the efficacies of antimicrobials for urethritis on microorganisms in the oral cavity. METHODS: Ninety-two male patients with urethritis and 17 male controls participated to this study at 12 urology clinics in Japan between March 2014 and March 2015. The first voided urine (FVU) and oral wash fluid (OWF) specimens were collected from the participants...
August 10, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28796760/acute-flaccid-myelitis-among-children-washington-september-november-2016
#11
Jesse Bonwitt, Amy Poel, Chas DeBolt, Elysia Gonzales, Adriana Lopez, Janell Routh, Krista Rietberg, Natalie Linton, James Reggin, James Sejvar, Scott Lindquist, Catherine Otten
In October 2016, Seattle Children's Hospital notified the Washington State Department of Health (DOH) and CDC of a cluster of acute onset of limb weakness in children aged ≤14 years. All patients had distinctive spinal lesions largely restricted to gray matter detected by magnetic resonance imaging (MRI), consistent with acute flaccid myelitis (AFM). On November 3, DOH issued a health advisory to local health jurisdictions requesting that health care providers report similar cases. By January 24, 2017, DOH and CDC had confirmed 10 cases of AFM and excluded two suspected cases among residents of Washington during September-November 2016...
August 11, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28794183/french-prospective-clinical-evaluation-of-the-aptima%C3%A2-mycoplasma-genitalium-assay-ce-ivd-and-macrolide-resistance-detection-using-three-distinct-assays
#12
Chloé Le Roy, Sabine Pereyre, Nadège Hénin, Cécile Bébéar
The aim of this study was to evaluate the clinical performance of the Aptima® Mycoplasma genitalium CE-IVD (MG-TMA) assay for the detection of Mycoplasma genitalium in clinical male and female samples in comparison with the in-house real-time PCR (in-house PCR) assay routinely used in our laboratory. A total of 1,431 clinical specimens obtained from 1,235 patients were prospectively collected at the Bacteriology Department of Bordeaux University Hospital (France). Additional research-use-only Aptima® M. genitalium TMA assays, Alt1-TMA and Alt2-TMA, were performed on discordant specimens to determine the M...
August 9, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28792486/trmfo-a-fibronectin-binding-adhesin-of-mycoplasma-bovis
#13
Yongpeng Guo, Hongmei Zhu, Jiayao Wang, Jing Huang, Farhan Anwar Khan, Jingjing Zhang, Aizhen Guo, Xi Chen
Mycoplasma bovis is an important pathogenic mycoplasma, causing the cattle industry serious economic losses. Adhesion is a crucial step in the mycoplasmas' infection and colonization process; fibronectin (Fn), an extracellular matrix glycoprotein, is a molecular bridge between the bacterial adhesins and host cell receptors. The present study was designed to characterize the Fn-binding ability of methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (TrmFO) and its role in M. bovis cytoadherence. The trmFO (MBOV_RS00785) gene was cloned and expressed in E...
August 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28780111/bulk-tank-milk-antibody-enzyme-linked-immunosorbent-assay-as-a-biosecurity-tool-for-detecting-dairy-herds-with-past-exposure-to-mycoplasma-bovis
#14
A M Parker, J K House, M S Hazelton, K L Bosward, J M Morton, P A Sheehy
In Australia, one of the biosecurity recommendations to help prevent the introduction of Mycoplasma bovis into a dairy herd is to use a PCR assay on bulk tank milk (BTM) samples to evaluate the M. bovis infection status of potential source herds. An alternative approach is to assess the immunological status of the herd with respect to previous exposure to M. bovis via the use of an ELISA that is commercially available for use on cattle milk and serum. The objectives of this study were to (1) evaluate factors potentially associated with variation in the ELISA BTM optical density coefficient (ODC%) in previously exposed herds, (2) evaluate the association between the proportion of cows that are ELISA positive and the BTM ELISA ODC%, (3) assess agreement between the BTM ELISA and PCR and culture, and (4) compare BTM ELISA ODC% between the "hospital" herd and the main lactating herd on the same farm...
August 2, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28777207/five-year-study-of-viral-etiology-and-features-of-febrile-respiratory-tract-infections-with-prolonged-fever-in-japanese-pediatric-outpatients
#15
Michimaru Hara, Shinichi Takao
Over 5 years, we prospectively collected nasopharyngeal aspirate samples from pediatric outpatients with prolonged fever (≥ 5 days, ≥ 38.0 °C). Real-time PCR assays identifying 13 different respiratory viruses and mycoplasma pneumoniae were performed on the test samples. Real-time PCR assays identified at least one pathogen in 273 (75.4%) of the 362 samples assessed (239 single and 34 multiple infections).
August 1, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28768998/synthesis-and-biological-evaluation-of-novel-thioether-pleuromutilin-derivatives
#16
Shuhua Mu, Huixian Liu, Lifang Zhang, Xiaoyang Wang, Feiqun Xue, Yue Zhang
To develop new pleuromutilin derivatives as veterinary antibiotic medicines, we designed and synthesized a series of new thioether pleuromutilin derivatives possessing acylthiazolyl moiety based on previously designed derivatives. The antibacterial properties of the prepared pleuromutilin derivatives were assessed in vitro by the broth dilution method against five kinds of bacteria and the mycoplasma Mycoplasma gallisepticum (MG). All of the tested compounds displayed moderate to good antibacterial activity to methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-sensitive Staphylococcus epidermidis (MSSE), methicillin-resistant S...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28766063/host-responses-to-pathogen-priming-in-a-natural-songbird-host
#17
Ariel E Leon, Dana M Hawley
Hosts in free-living populations can experience substantial variation in the frequency and dose of pathogen exposure, which can alter disease progression and protection from future exposures. In the house finch-Mycoplasma gallisepticum (MG) system, the pathogen is primarily transmitted via bird feeders, and some birds may be exposed to frequent low doses of MG while foraging. Here we experimentally determined how low dose, repeated exposures of house finches to MG influence host responses and protection from secondary high-dose challenge...
August 1, 2017: EcoHealth
https://www.readbyqxmd.com/read/28765500/infectious-keratoconjunctivitis-and-occurrence-of-mycoplasma-conjunctivae-and-chlamydiaceae-in-small-domestic-ruminants-from-central-karakoram-pakistan
#18
Xavier Fernández-Aguilar, Luca Rossi, Óscar Cabezón, Andrea Giorgino, Isis Victoriano Llopis, Joachim Frey, Jorge Ramón López-Olvera
Infectious keratoconjunctivitis (IKC) is a contagious eye disease primarily caused by Mycoplasma conjunctivae in domestic and wild Caprinae. Chlamydophila species have also been detected in ruminants with IKC. The objectives of this study are to investigate the ocular infection of M. conjunctivae and Chlamydiaceae and assess its interaction in relation to IKC in sheep and goats from remote communities around the Central Karakoram National Park in Pakistan, performing a combination of cross-sectional and case-control study design...
August 1, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28762558/mycoplasma-genitalium-an-agent-of-reemerging-sexually-transmitted-infections
#19
Sophie Edouard, Hervé Tissot-Dupont, Grégory Dubourg, Annick Bernard, Pierre-Edouard Fournier, Isabelle Ravaux, Andreas Stein, Didier Raoult
M. genitalium is a reemerging microorganism, responsible for sexually transmissible infections (STIs), with prevalence which varies depending on the country and population group studied. We report here M. genitalium prevalence among the specimens received for STI diagnosis in our routine microbiological laboratory in the university hospital in Marseille, France. We tested 4 624 samples from 3 793 patients using qPCR for M. genitalium, C. trachomatis, N. gonorrheae, T. pallidum. Of these samples, 528 (13.6%) patients were tested positive for at least one pathogen and 126 (3...
August 1, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28760082/mycoplasma-pneumoniae-23s-rrna-a2063g-mutation-does-not-influence-chest-radiography-features-in-children-with-pneumonia
#20
Huan Deng, Jun Rui, Deyu Zhao, Feng Liu
Objective To measure the rate of the A2063G mutation in the Mycoplasma pneumoniae ( M. pneumoniae) 23S rRNA domain V in children with pneumonia and to determine the correlation between radiographic findings and the presence of the A2063G mutation. Methods Patients who were hospitalized with a confirmed diagnosis of M. pneumoniae pneumonia were enrolled in this study. M. pneumoniae strains were collected for genotype analysis. Chest radiography was performed on all children prior to and following macrolide treatment...
January 1, 2017: Journal of International Medical Research
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