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Haemophilus parasuis

Jiang Yu, Yuyu Zhang, Yijun Du, Jun Li, Baohua Huang, Wenbo Sun, Xiaoyan Cong, Jun Peng, Sufang Ren, Lihui Gou, Jianli Shi, Zhi Chen, Lei Chen, Jianda Li, Jie Yang, Jiaqiang Wu, Jinbao Wang
The use of BALB/c mouse as an alternative model to study Haemophilus parasuis (HPS) infections was evaluated, supplying the serotyping scheme by comparing the pathogenicity of different serovar HPS in pigs and mice challenge using statistical analysis. Results showed that the pathogenicity of different serovar HPS in mouse was consistent with in pigs, proving that this model is a viable alternative to pigs. It provides a convenient methodology for determining the virulence of HPS strains.
October 24, 2016: Research in Veterinary Science
Sandra Prüller, Conny Turni, Patrick J Blackall, Martin Beyerbach, Günter Klein, Lothar Kreienbrock, Katrin Strutzberg-Minder, Heike Kaspar, Diana Meemken, Corinna Kehrenberg
Currently, there is no agreed method available for broth microdilution susceptibility testing of Haemophilus parasuis, one of the most important bacterial pathogens in pig production. Therefore, the aim of the study was to develop a method that could be easily performed by diagnostic laboratories and that appears suitable for a harmonized susceptibility testing.Growth determinations using one type strain and three field isolates revealed no visible growth of H. parasuis in media which have proven to be suitable for susceptibility testing of fastidious organisms...
November 9, 2016: Journal of Clinical Microbiology
Yushan Chen, Shanshan Zhou, Kexin Hua, Hongde Xiao, Zili Li, Mei Liu, Rui Luo, Dingren Bi, Rui Zhou, Hui Jin
No abstract text is available yet for this article.
October 10, 2016: Molecular Immunology
Zhanqin Zhao, Huisheng Liu, Keshan Zhang, Qiao Xue, Kunpeng Chen, Yun Xue
The objective of this study was to assess the minimum dose, antigen content, and immunization duration of a trivalent vaccine containing inactivated Haemophilus parasuis serovars 4, 5, and 12 and the Montanide GEL 01 PR adjuvant in piglets and pregnant sows. Our results demonstrated that the minimum vaccine dose was 2 mL per pig and the optimal antigen content 2.0 × 10(9), 1.0 × 10(9), and 1.0 × 10(9) colony-forming units/mL of serovars 4, 5, and 12, respectively. The vaccine provided effective protection 14 d after the 2nd vaccination, and the period of immune protection was 180 d (6 mo) after the 2nd vaccination...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
Junxing Li, Xiufang Yuan, Lihua Xu, Lei Kang, Jun Jiang, Yicheng Wang
Studies on virulence factors and pathogenecity of Haemophilus parasuis have long been hindered by a lack of a consistent system for genetic manipulation. In this study, competence was induced by transferring H. parasuis from rich medium to starvation medium media-IV (M-IV) and iscR gene deficient mutants of H. parasuis were generated efficiently. Transformation frequency varied from 4.1 × 10(-5) to 1.1 × 10(-8) when using circular plasmid, and increased to about 2- to 31-fold when transformed using linearized plasmid...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
Yaning Zhang, Yufeng Li, Wentao Yuan, Yuting Xia, Yijuan Shen
Haemophilus parasuis (H. parasuis) is a common commensal Gram-negative extracellular bacterium in the upper respiratory tract of swine, which can cause Glässer's disease in stress conditions. Research on the pathogenicity of H. parasuis has mainly focused on immune evasion and bacterial virulence factors, while few studies have examined the interactions of H. parasuis and its host. Autophagy is associated with the replication and proliferation of many pathogenic bacteria, but whether it plays a role during infection by H...
2016: Frontiers in Microbiology
Huisheng Liu, Qiao Xue, Qiaoying Zeng, Zhanqin Zhao
Glässer's disease, which is caused by Haemophilus parasuis, is a major threat to swine throughout the world. At present, the predominant method of controlling this disease is through vaccination with an inactivated vaccine, which has many limitations. For example, there is no available method to differentiate between infected and vaccinated animals. In addition, inactivated vaccines do not contain all of the virulent serovars that circulate in a region. Additionally, novel vaccines that address the shortcomings of inactivated vaccines are not available...
November 1, 2016: Veterinary Immunology and Immunopathology
Qi Zhou, Saixiang Feng, Jianmin Zhang, Aiqing Jia, Kaijie Yang, Kaixiang Xing, Ming Liao, Huiying Fan
Haemophilus parasuis is a common opportunistic pathogen known for its ability to colonize healthy piglets and causes Glässer's disease. The lipooligosaccharide (LOS) of H. parasuis is a potential virulence-associated factor. In this study, two putative glycosyltransferases that might be involved in LOS synthesis in H. parasuis SC096 were identified (lgtB and lex-1). Mutants were constructed to investigate the roles of the lgtB and lex-1 genes. The LOS from the ΔlgtB or Δlex-1 mutant showed truncated structure on silver-stained SDS-PAGE gel compared to the wild-type strain...
2016: Frontiers in Cellular and Infection Microbiology
Yushan Chen, Ting Liu, Paul Langford, Kexin Hua, Shanshan Zhou, Yajun Zhai, Hongde Xiao, Rui Luo, Dingren Bi, Hui Jin, Rui Zhou
No abstract text is available yet for this article.
October 2016: Molecular Immunology
S Martínez-Martínez, E F Rodríguez-Ferri, R Frandoloso, J J Garrido-Pavón, S Zaldívar-López, C Barreiro, C B Gutiérrez-Martín
The molecular analysis of pigs vaccinated with a mutant transferrin-binding protein B (Y167A) from Haemophilus parasuis was compared with that performed for unvaccinated challenged (UNCH) and unvaccinated unchallenged (UNUN) pigs. Microarray analysis revealed that UNCH group showed the most distinct expression profile for immune response genes, mainly for those genes involved in inflammation or immune cell trafficking. This fact was confirmed by real-time PCR, in which the greatest level of differential expression from this group were CD14, CD163, IL-8 and IL-12...
October 2016: Comparative Immunology, Microbiology and Infectious Diseases
Sonia Martínez-Martínez, Rafael Frandoloso, Elías-Fernando Rodríguez-Ferri, María-José García-Iglesias, Claudia Pérez-Martínez, Álvaro Álvarez-Estrada, César-Bernardo Gutiérrez-Martín
This study aimed to characterize the type of immune response induced by an experimental vaccine based on a mutant Haemophilus parasuis transferrin binding protein (Tbp) B (Y167A) defective in its ability to bind porcine transferrin. Clinical and pathological signs, bacterial clearance, antibody response and the cytokine profile in alveolar macrophages and spleen after the vaccination and challenge of twenty-two colostrum-deprived pigs with 10(8) CFU of H. parasuis were analysed. Pigs vaccinated with Y167A were compared to those vaccinated with native TbpB (nTbpB), those treated with a commercial bacterin (CB) against Glässer's disease, those unvaccinated challenged (CH) and those unvaccinated unchallenged (UNCH) pigs...
October 15, 2016: Veterinary Immunology and Immunopathology
Yeotaek Cheong, Changin Oh, Kunkyu Lee, Ki-Hyun Cho
Oral fluid analysis for monitoring herd is an interest in commercial pig farms in Korea. The aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (PRDC) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. The samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (RT-PCR) or standard PCR depending on microorganisms. The pathogens were divided into virus group and bacteria group...
September 1, 2016: Journal of Veterinary Science
Shulin Fu, Lei Xu, Sali Li, Yinsheng Qiu, Yu Liu, Zhongyuan Wu, Chun Ye, Yongqing Hou, Chien-An Andy Hu
Haemophilus parasuis (H. parasuis) is the causative agent of Glässer's disease, a severe membrane inflammation disorder. Previously we showed that Baicalin (BA) possesses anti-inflammatory effects via the NLRP3 inflammatory pathway in an LPS-challenged piglet model. However, whether BA has anti-inflammatory effects upon H. parasuis infection is still unclear. This study investigated the anti-inflammatory effects and mechanisms of BA on H. parasuis-induced inflammatory responses via the NF-κB and NLRP3 inflammasome pathway in piglet mononuclear phagocytes (PMNP)...
2016: Veterinary Research
Nubia Macedo, Simone Oliveira, Montserrat Torremorell, Albert Rovira
Haemophilus parasuis is an important swine pathogen that causes Glasser's disease, characterized by pneumonia, polyserositis and meningitis. Protection against H. parasuis infection is associated with the presence of homologous antibodies in serum. However, a H. parasuis antigen that can elicit a protective immune response against all H. parasuis strains has yet to be found. A novel immunogenic and species-specific H. parasuis protein was identified by screening H. parasuis whole cell proteins using swine convalescent sera...
August 2016: Research in Veterinary Science
Ke Dai, Jin Jin, Yiping Wen, Xintian Wen, Lvqin He, Sanjie Cao, Xiaobo Huang, Rui Wu, Qin Zhao
Haemophilus parasuis, a normal Gram-negative bacterium, may cause Glässer's disease and pneumonia in pigs. This study aims to identify the genes related to natural competence of the serotype 11 strain SC1401, which frequently shows competence and high pathogenicity. SC1401 shows many differences from strains without natural competence within the molecular basis. We performed complete genome sequencing together with restriction modification system analysis to lay the foundation for later study.
2016: Genome Announcements
Liyan Wang, Lina Ma, Yongan Liu, Pengcheng Gao, Youquan Li, Xuerui Li, Yongsheng Liu
Haemophilus parasuis is the etiological agent of Glässers disease, which causes high morbidity and mortality in swine herds. Although H. parasuis strains can be classified into 15 serovars with the Kielstein-Rapp-Gabrielson serotyping scheme, a large number of isolates cannot be classified and have been designated 'nontypeable' strains. In this study, multilocus sequence typing (MLST) of H. parasuis was used to analyze 48 H. parasuis field strains isolated in China and two strains from Australia. Twenty-six new alleles and 29 new sequence types (STs) were detected, enriching the H...
October 2016: Infection, Genetics and Evolution
Jian Xu, Fei Zhong, Yonghong Zhang, Jianlou Zhang, Shanshan Huo, Hongyu Lin, Liyue Wang, Dan Cui, Xiujin Li
Probiotics and antimicrobial peptides (AMPs) in animal gastrointestinal tract play the crucial roles in microbiota maintenance, immune regulation and pathogen defending. Generating probiotics strain engineered for expressing AMPs should be beneficial for animal growth and production. Here, we generated B. subtilis strain engineered for expressing porcine β-defensin-2 (pBD-2) and cecropin P1 (CP1) fusion antimicrobial peptide using their codon-optimized genes mediated by pMK4 prokaryotic expression vector. Results showed that the B...
June 30, 2016: Asian-Australasian Journal of Animal Sciences
Yiping Wen, Xuefeng Yan, Yiping Wen, Sanjie Cao, Lvqin He, Lingqiang Ding, Luhua Zhang, Peng Zhou, Xiaobo Huang, Rui Wu, Xintian Wen
Haemophilus parasuis causes serious economic losses in pigs, which is the etiology of Glässer's disease. In this study we studied the immunogenicity of proteins encoded by the hxuCBA gene cluster in H. parasuis. Through bioinformatics analysis, HxuC, HxuB, and HxuA proteins were found that they might have strong antigenicity, with 31 putative cytotoxic T-lymphocyte (CTL) epitopes and multiple B-cell antigenic determinants. Western blotting assay indicated that all these antigens are highly immunogenic. The antibody levels and the levels of IL-2, IL-4, IFN-γ in the groups of HxuA, HxuB, HxuC, HxuCBA (include HxuC, HxuB and HxuA proteins), and M-3 were observed to significantly increase with time post vaccination...
October 15, 2016: Gene
Rune T Nordvang, Christian Nyffenegger, Jesper Holck, Carsten Jers, Birgitte Zeuner, Ulrik K Sundekilde, Anne S Meyer, Jørn D Mikkelsen
Sialidases ( may exhibit trans-sialidase activity to catalyze sialylation of lactose if the active site topology is congruent with that of the Trypanosoma cruzi trans-sialidase (EC 2.4.1.-). The present work was undertaken to test the hypothesis that a particular aromatic sandwich structure of two amino acids proximal to the active site of the T. cruzi trans-sialidase infers trans-sialidase activity. On this basis, four enzymes with putative trans-sialidase activity were identified through an iterative alignment from 2909 native sialidases available in GenBank, which were cloned and expressed in Escherichia coli...
2016: PloS One
Luisa Z Moreno, Givago F R Silva, Vasco T M Gomes, Carlos E C Matajira, Ana Paula S Silva, Renan E Mesquita, Nicholas P Lotto, Thais S P Ferreira, Ana Paula G Christ, Maria Inês Z Sato, Yuri Gherpelli, Michele Dottori, Paolo Bonilauri, Andrea Luppi, Andrea M Moreno
No abstract text is available yet for this article.
2016: Journal of Infection in Developing Countries
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