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Haemophilus parasuis

Huisheng Liu, Zhanqin Zhao, Xiaojian Xi, Qiao Xue, Ta Long, Yun Xue
BACKGROUND: Prior to the 1990s, P. multocida capsular serogroup A was the most prevalent in China, followed by serogroups B and D. Thirty years later, serogroup D became the most prevalent, followed by serogroups A and B. However, the P. multocida capsular serogroups currently circulating in China remain unclear. Therefore, the aim of the present study was to provide an update on P. multocida serogroups isolated from diagnostic samples collected from clinically diseased pigs in Central and Eastern China from 2011 to 2015...
2017: Irish Veterinary Journal
Florencia Correa-Fiz, Nuria Galofre-Mila, Mar Costa-Hurtado, Virginia Aragon
Haemophilus parasuis is a bacterium from the Pasteurellaceae family that comprises strains of different degree of virulence. Non-virulent strains are considered components of the upper respiratory tract microbiota, while virulent strains can invade systemic organs and cause fibrinous polyserositis (Glässer's disease). Genomic comparison of virulent and non-virulent strains led to the identification of a family of genes differentially associated to virulence, the virulence-associated trimeric autotransporters (vtaA)...
January 2017: Veterinary Microbiology
Lina Ma, Liyan Wang, Yuefeng Chu, Xuerui Li, Yujun Cui, Shengli Chen, Jianhua Zhou, Chunling Li, Zhongxin Lu, Jixing Liu, Yongsheng Liu
Haemophilus parasuis is classified mainly through serotyping, but traditional serotyping always yields non-typable (NT) strains and unreliable results via cross-reactions. Here, we surveyed the serotype prevalence of Chinese H. parasuis isolates using traditional serotyping (gel immuno-diffusion test, GID) and molecular serotyping (multiplex PCR, mPCR). We also investigated why discrepant results between these methods were obtained, and investigated mPCR failure through whole-genome sequencing. Of the 100 isolate tested, 73 (73%) and 93 (93%) were serotyped by the GID test and mPCR, respectively, with a concordance rate of 66% (66/100)...
2016: PloS One
Liangyou Zhao, Xueli Gao, Chaonan Liu, Xiaoping Lv, Nan Jiang, Shimin Zheng
Haemophilus parasuis is an important pathogen causing severe infections in pigs. However, the specific bacterial factors that participate in pathogenic process are poorly understood. VacJ protein is a recently discovered outer membrane lipoprotein that relates to virulence in several pathogens. To characterize the function of the vacJ gene in H. parasuis virulent strain HS49, a vacJ gene-deletion mutant ΔvacJ and its complemented strain were constructed. Our findings supported that VacJ is essential for maintenance of cellular integrity and stress tolerance of H...
March 1, 2017: Gene
Lingqiang Ding, Xintian Wen, Lvqin He, Xuefeng Yan, Yongping Wen, Sanjie Cao, Xiaobo Huang, Rui Wu, Yiping Wen
As a global transcriptional factor, ArcA regulates the expression of hundreds of genes involved in aerobic and anaerobic metabolism. Here we deleted arcA gene and investigated the biological characteristics of arcA deletion mutant (ΔarcA) in Haemophilus parasuis (H. parasuis) serovar 13 clinical strain EP3. Results indicated that deletion of arcA impaired growth of EP3 strain under anaerobic condition, and reduced virulence of EP3 strain in mice. Additionally, the ΔarcA strain showed greater sensitivity in porcine serum and produced less biofilm mass than the EP3 strain...
November 30, 2016: Veterinary Microbiology
Yufeng Li, Yaning Zhang, Yuting Xia, Yijuan Shen, Jiansong Zhang
TGF-β1 plays an important role during the invasion of some bacteria into cells by regulating the expression of integrins, fibronectin, and other extracellular matrix proteins. We postulated that TGF-β1 levels could affect the invasion of Haemophilus parasuis into PK-15 cells. After infection by H. parasuis, PK-15 cells had elevated levels of TGF-β1 expression. Treatment of PK-15 cells with TGF-β1 prior to infection significantly decreased invasion by H. parasuis. Both TGF-β1 treatment and H. parasuis infection resulted in significant induction of fibronectin (Fn) and α5 integrin...
November 30, 2016: Veterinary Microbiology
Marion Brean, Sam Abraham, Michelle Hebart, Roy N Kirkwood
Litters of primiparous and multiparous sows were switched at 12 d and nasally swabbed at 12, 18, and 23 d for detection of Haemophilus parasuis. At lactation days 12 and 23, mucosal colonization rates for multiparous and primiparous litters were 0% versus 33% and 26% versus 60%, respectively.
December 2016: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
Jiang Yu, Yuyu Zhang, Yijun Du, Jun Li, Baohua Huang, Wenbo Sun, Xiaoyan Cong, Jun Peng, Sufang Ren, Lihui Gou, Jianli Shi, Zhi Chen, Lei Chen, Jianda Li, Jie Yang, Jiaqiang Wu, Jinbao Wang
The use of BALB/c mouse as an alternative model to study Haemophilus parasuis (HPS) infections was evaluated, supplying the serotyping scheme by comparing the pathogenicity of different serovar HPS in pigs and mice challenge using statistical analysis. Results showed that the pathogenicity of different serovar HPS in mouse was consistent with in pigs, proving that this model is a viable alternative to pigs. It provides a convenient methodology for determining the virulence of HPS strains.
December 2016: Research in Veterinary Science
Sandra Prüller, Conny Turni, Patrick J Blackall, Martin Beyerbach, Günter Klein, Lothar Kreienbrock, Katrin Strutzberg-Minder, Heike Kaspar, Diana Meemken, Corinna Kehrenberg
Currently, there is no agreed method available for broth microdilution susceptibility testing of Haemophilus parasuis, one of the most important bacterial pathogens in pig production. Therefore, the aim of this study was to develop a method that could be easily performed by diagnostic laboratories and that appears suitable for a harmonized susceptibility testing. Growth determinations using one type strain and three field isolates revealed no visible growth of H. parasuis in media which have proven to be suitable for susceptibility testing of fastidious organisms...
January 2017: Journal of Clinical Microbiology
Yushan Chen, Shanshan Zhou, Kexin Hua, Hongde Xiao, Zili Li, Mei Liu, Rui Luo, Dingren Bi, Rui Zhou, Hui Jin
No abstract text is available yet for this article.
November 2016: Molecular Immunology
Zhanqin Zhao, Huisheng Liu, Keshan Zhang, Qiao Xue, Kunpeng Chen, Yun Xue
The objective of this study was to assess the minimum dose, antigen content, and immunization duration of a trivalent vaccine containing inactivated Haemophilus parasuis serovars 4, 5, and 12 and the Montanide GEL 01 PR adjuvant in piglets and pregnant sows. Our results demonstrated that the minimum vaccine dose was 2 mL per pig and the optimal antigen content 2.0 × 10(9), 1.0 × 10(9), and 1.0 × 10(9) colony-forming units/mL of serovars 4, 5, and 12, respectively. The vaccine provided effective protection 14 d after the 2nd vaccination, and the period of immune protection was 180 d (6 mo) after the 2nd vaccination...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
Junxing Li, Xiufang Yuan, Lihua Xu, Lei Kang, Jun Jiang, Yicheng Wang
Studies on virulence factors and pathogenecity of Haemophilus parasuis have long been hindered by a lack of a consistent system for genetic manipulation. In this study, competence was induced by transferring H. parasuis from rich medium to starvation medium media-IV (M-IV) and iscR gene deficient mutants of H. parasuis were generated efficiently. Transformation frequency varied from 4.1 × 10(-5) to 1.1 × 10(-8) when using circular plasmid, and increased to about 2- to 31-fold when transformed using linearized plasmid...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
Yaning Zhang, Yufeng Li, Wentao Yuan, Yuting Xia, Yijuan Shen
Haemophilus parasuis (H. parasuis) is a common commensal Gram-negative extracellular bacterium in the upper respiratory tract of swine, which can cause Glässer's disease in stress conditions. Research on the pathogenicity of H. parasuis has mainly focused on immune evasion and bacterial virulence factors, while few studies have examined the interactions of H. parasuis and its host. Autophagy is associated with the replication and proliferation of many pathogenic bacteria, but whether it plays a role during infection by H...
2016: Frontiers in Microbiology
Huisheng Liu, Qiao Xue, Qiaoying Zeng, Zhanqin Zhao
Glässer's disease, which is caused by Haemophilus parasuis, is a major threat to swine throughout the world. At present, the predominant method of controlling this disease is through vaccination with an inactivated vaccine, which has many limitations. For example, there is no available method to differentiate between infected and vaccinated animals. In addition, inactivated vaccines do not contain all of the virulent serovars that circulate in a region. Additionally, novel vaccines that address the shortcomings of inactivated vaccines are not available...
November 1, 2016: Veterinary Immunology and Immunopathology
Qi Zhou, Saixiang Feng, Jianmin Zhang, Aiqing Jia, Kaijie Yang, Kaixiang Xing, Ming Liao, Huiying Fan
Haemophilus parasuis is a common opportunistic pathogen known for its ability to colonize healthy piglets and causes Glässer's disease. The lipooligosaccharide (LOS) of H. parasuis is a potential virulence-associated factor. In this study, two putative glycosyltransferases that might be involved in LOS synthesis in H. parasuis SC096 were identified (lgtB and lex-1). Mutants were constructed to investigate the roles of the lgtB and lex-1 genes. The LOS from the ΔlgtB or Δlex-1 mutant showed truncated structure on silver-stained SDS-PAGE gel compared to the wild-type strain...
2016: Frontiers in Cellular and Infection Microbiology
Yushan Chen, Ting Liu, Paul Langford, Kexin Hua, Shanshan Zhou, Yajun Zhai, Hongde Xiao, Rui Luo, Dingren Bi, Hui Jin, Rui Zhou
No abstract text is available yet for this article.
October 2016: Molecular Immunology
S Martínez-Martínez, E F Rodríguez-Ferri, R Frandoloso, J J Garrido-Pavón, S Zaldívar-López, C Barreiro, C B Gutiérrez-Martín
The molecular analysis of pigs vaccinated with a mutant transferrin-binding protein B (Y167A) from Haemophilus parasuis was compared with that performed for unvaccinated challenged (UNCH) and unvaccinated unchallenged (UNUN) pigs. Microarray analysis revealed that UNCH group showed the most distinct expression profile for immune response genes, mainly for those genes involved in inflammation or immune cell trafficking. This fact was confirmed by real-time PCR, in which the greatest level of differential expression from this group were CD14, CD163, IL-8 and IL-12...
October 2016: Comparative Immunology, Microbiology and Infectious Diseases
Sonia Martínez-Martínez, Rafael Frandoloso, Elías-Fernando Rodríguez-Ferri, María-José García-Iglesias, Claudia Pérez-Martínez, Álvaro Álvarez-Estrada, César-Bernardo Gutiérrez-Martín
This study aimed to characterize the type of immune response induced by an experimental vaccine based on a mutant Haemophilus parasuis transferrin binding protein (Tbp) B (Y167A) defective in its ability to bind porcine transferrin. Clinical and pathological signs, bacterial clearance, antibody response and the cytokine profile in alveolar macrophages and spleen after the vaccination and challenge of twenty-two colostrum-deprived pigs with 10(8) CFU of H. parasuis were analysed. Pigs vaccinated with Y167A were compared to those vaccinated with native TbpB (nTbpB), those treated with a commercial bacterin (CB) against Glässer's disease, those unvaccinated challenged (CH) and those unvaccinated unchallenged (UNCH) pigs...
October 15, 2016: Veterinary Immunology and Immunopathology
Yeotaek Cheong, Changin Oh, Kunkyu Lee, Ki-Hyun Cho
Oral fluid analysis for monitoring herd is an interest in commercial pig farms in Korea. The aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (PRDC) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. The samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (RT-PCR) or standard PCR depending on microorganisms. The pathogens were divided into virus group and bacteria group...
September 1, 2016: Journal of Veterinary Science
Shulin Fu, Lei Xu, Sali Li, Yinsheng Qiu, Yu Liu, Zhongyuan Wu, Chun Ye, Yongqing Hou, Chien-An Andy Hu
Haemophilus parasuis (H. parasuis) is the causative agent of Glässer's disease, a severe membrane inflammation disorder. Previously we showed that Baicalin (BA) possesses anti-inflammatory effects via the NLRP3 inflammatory pathway in an LPS-challenged piglet model. However, whether BA has anti-inflammatory effects upon H. parasuis infection is still unclear. This study investigated the anti-inflammatory effects and mechanisms of BA on H. parasuis-induced inflammatory responses via the NF-κB and NLRP3 inflammasome pathway in piglet mononuclear phagocytes (PMNP)...
2016: Veterinary Research
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