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Haemophilus parasuis

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https://www.readbyqxmd.com/read/28448603/evaluation-of-immunogenicity-and-protective-efficacy-of-recombinant-outer-membrane-proteins-of-haemophilus-parasuis-serovar-5-in-a-murine-model
#1
Miao Li, Ru-Jian Cai, Shuai Song, Zhi-Yong Jiang, Yan Li, Hong-Chao Gou, Pin-Pin Chu, Chun-Ling Li, Hua-Ji Qiu
Glässer's disease is an economically important infectious disease of pigs caused by Haemophilus parasuis. Few vaccines are currently available that could provide effective cross-protection against various serovars of H. parasuis. In this study, five OMPs (OppA, TolC, HxuC, LppC, and HAPS_0926) identified by bioinformatic approaches, were cloned and expressed as recombinant proteins. Antigenicity of the purified proteins was verified through Western blotting, and primary screening for protective potential was evaluated in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28385011/pharmacokinetics-of-tilmicosin-in-healthy-pigs-and-in-pigs-experimentally-infected-with-haemophilus-parasuis
#2
Ling Zhang, Li Zhao, Yonghong Liu, Junfeng Liu, Xianqiang Li
A comparative in vivo pharmacokinetic (PK) study of tilmicosin (TIL) was conducted in 6 crossbred healthy pigs and 6 crossbred pigs infected with Haemophilus parasuis (H. parasuis) following oral administration of a single dose of 40 mg/kg. The infected model was established by intranasal inoculation and confirmed by clinical signs, blood biochemical and microscopic examination. TIL concentrations in plasma were determined by a validated high-performance liquid chromatography (HPLC) method with ultra violet detection at a wavelength of 285 nm and PK parameters were calculated by using the Winnonlin software...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28361037/either-fadd1-or-fadd2-which-encode-acyl-coa-synthetase-is-essential-for-the-survival-of-haemophilus-parasuis-sc096
#3
Saixiang Feng, Chenggang Xu, Kaijie Yang, Haihong Wang, Huiying Fan, Ming Liao
In Haemophilus parasuis, the genes HAPS_0217 and HAPS_1695 are predicted to encode long-chain fatty acid-CoA ligases (FACSs). These proteins contain ATP/AMP signature motifs and FACS conserved motifs that are homologous to those in Escherichia coli FadD (EcFadD). In this study, we demonstrate that HAPS_0217 and HAPS_1695 can functionally replace EcFadD in the E. coli fadD mutant JW1794, and were thus designated fadD1 and fadD2, respectively. An evaluation of kinetic parameters indicated that FadD1 and FadD2 have a substrate preference for long-chain fatty acids...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28348142/survey-of-susceptibility-to-marbofloxacin-in-bacteria-isolated-from-diseased-pigs-in-europe
#4
F El Garch, S Kroemer, D Galland, I Morrissey, F Woehrle
A monitoring programme of marbofloxacin susceptibility of bacteria from Europe causing respiratory tract infection and meningitis in pigs has been active since 1994 and 2002, respectively. Monitoring digestive, metritis and urinary tract infection (UTI) in pigs has been active since 2005 and susceptibility results until 2013 are presented. Minimum inhibitory concentration (MIC) was determined by broth microdilution. For MIC interpretation, Vétoquinol-evaluated breakpoints were applied. For digestive pathogens, Escherichia coli and Salmonella species (1717 and 300 isolates, respectively) exhibited 7...
March 27, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28254554/the-extended-leader-peptide-of-haemophilus-parasuis-trimeric-autotransporters-conditions-their-protein-expression-in-escherichia-coli
#5
Sonia Pina-Pedrero, Àlex Olvera, Albert Bensaid
Trimeric autotransporters are surface-exposed proteins of Gram-negative bacteria belonging to the type V secretion system. They are involved in virulence and are targets for vaccine and diagnostic tool development, so optimal systems for their expression and purification are required. In the present study, the impact of the extended leader peptide of the Haemophilus parasuis virulence-associated trimeric autotransporters (VtaA) in its production as recombinant proteins in Escherichia coli was evaluated. The 13 genes encoding the VtaA1 to VtaA13 passenger domains of the strain Nagasaki were cloned in the pASK-IBA33plus plasmid and expressed in E...
February 28, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28187951/omp16-based-vaccine-encapsulated-by-alginate-chitosan-microspheres-provides-significant-protection-against-haemophilus-parasuis-in-mice
#6
Xintian Zheng, Xiaoyan Yang, Xiaohua Li, Guo-Hua Qiu, Ailing Dai, Qichun Huang, Cuiqin Huang, Xiaofeng Guo
Haemophilus parasuis (H. parasuis) is the etiological agent of swine Glässer's disease, which leads to significant economic loss in swine industry over the world. Subunit vaccine based on outer membrane protein is one of the promising choices to protect pigs against H. parasuis infection despite low immunity efficiency. In this paper, outer membrane protein 16 (Omp16) of H. parasuis encapsulated by alginate-chitosan microspheres as antigen carriers was explored for the first time in a mouse model. Our results showed that the microspheres with Omp16 induced significant higher H...
February 7, 2017: Vaccine
https://www.readbyqxmd.com/read/28177794/evaluation-of-recombinant-protein-superoxide-dismutase-of-haemophilus-parasuis-strain-sh0165-as-vaccine-candidate-in-a-mouse-model
#7
Ling Guo, Lei Xu, Tao Wu, Shulin Fu, Yinsheng Qiu, Chien-An Andy Hu, Xinglong Ren, Rongrong Liu, Mengdie Ye
Haemophilus parasuis can cause a severe membrane inflammation disorder. It has been documented that superoxide dismutase (SOD) is a potential target to treat systemic inflammatory diseases. Therefore, we constructed an experimental H. parasuis subunit vaccine SOD and determined the protective efficacy of SOD using a lethal dose challenge against H. parasuis serovar 4 strain MD0322 and serovar 5 strain SH0165 in a mouse model. The results demonstrated that SOD could induce a strong humoral immune response in mice and provide significant immunoprotection efficacy against a lethal dose of H...
April 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28154458/analysis-of-efficacy-obtained-with-a-trivalent-inactivated-haemophilus-parasuis-serovars-4-5-and-12-vaccine-and-commercial-vaccines-against-gl%C3%A3-sser-s-disease-in-piglets
#8
Zhanqin Zhao, Huisheng Liu, Yun Xue, Kunpeng Chen, Zhijun Liu, Qiao Xue, Chen Wang
The objective of this study was to assess the efficacy of a trivalent inactivated Haemophilus parasuis serovars 4, 5, and 12 vaccine with polymeric adjuvant gel (GEL) and commercial vaccines against Glässer's disease in piglets. Commercial vaccines containing inactivated H. parasuis serovars 4 and 5 (China), inactivated H. parasuis serovars 1 and 6 (Spain), and inactivated H. parasuis serovar 5 (USA) were also evaluated. Our results demonstrated that the trivalent inactivated H. parasuis serovars 4, 5, and 12 vaccine with GEL adjuvant can provide better protection against the 3 most common pathogenic serovars circulating in China than other commercial vaccines tested...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28145487/the-antibacterial-activities-of-aditoprim-and-its-efficacy-in-the-treatment-of-swine-streptococcosis
#9
Guyue Cheng, Yamei Xu, Xudong Zhu, Shuyu Xie, Liye Wang, Lingli Huang, Haihong Hao, Zhenli Liu, Yuanhu Pan, Dongmei Chen, Yulian Wang, Zonghui Yuan
Aditoprim (ADP) has potential use as an antimicrobial agent in animals. However, its pharmacodynamic properties have not been systematically studied yet. In this study, the in vitro antibacterial activities of ADP and its main metabolites were assayed, and the in vivo antibacterial efficacy of ADP for the treatment of swine streptococcosis was evaluated. It was shown that Salmonella and Streptococcus from swine, Escherichia coli and Salmonella from chickens, E. coli, Streptococcus, Mannheimia, Pasteurella from calves, Streptococcus and Mannheimia from sheep, and E...
February 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28110792/effect-of-enrofloxacin-on-haemophilus-parasuis-infection-disease-and-immune-response
#10
Nubia Macedo, Maxim C J Cheeran, Albert Rovira, Andrew Holtcamp, Montserrat Torremorell
Haemophilus parasuis, the causative agent of Glasser's disease, is a pathogen that colonizes the upper respiratory tract (URT) of pigs, invades the bloodstream and causes polyserositis. Because antimicrobials are highly effective against H. parasuis, we hypothesized that they could have a detrimental effect on the establishment of an immune response if given at the time of URT colonization. In this study, we characterized clinical outcomes and antibody and IFN-γ responses to H. parasuis in pigs treated with enrofloxacin before or after low dose inoculation with a pathogenic H...
February 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28078081/occurrence-of-pasteurella-multocida-among-pigs-with-respiratory-disease-in-china-between-2011-and-2015
#11
Huisheng Liu, Zhanqin Zhao, Xiaojian Xi, Qiao Xue, Ta Long, Yun Xue
BACKGROUND: Prior to the 1990s, P. multocida capsular serogroup A was the most prevalent in China, followed by serogroups B and D. Thirty years later, serogroup D became the most prevalent, followed by serogroups A and B. However, the P. multocida capsular serogroups currently circulating in China remain unclear. Therefore, the aim of the present study was to provide an update on P. multocida serogroups isolated from diagnostic samples collected from clinically diseased pigs in Central and Eastern China from 2011 to 2015...
2017: Irish Veterinary Journal
https://www.readbyqxmd.com/read/28062001/identification-of-a-surface-epitope-specific-of-virulent-strains-of-haemophilus-parasuis
#12
Florencia Correa-Fiz, Nuria Galofre-Mila, Mar Costa-Hurtado, Virginia Aragon
Haemophilus parasuis is a bacterium from the Pasteurellaceae family that comprises strains of different degree of virulence. Non-virulent strains are considered components of the upper respiratory tract microbiota, while virulent strains can invade systemic organs and cause fibrinous polyserositis (Glässer's disease). Genomic comparison of virulent and non-virulent strains led to the identification of a family of genes differentially associated to virulence, the virulence-associated trimeric autotransporters (vtaA)...
January 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28005999/characterization-of-chinese-haemophilus-parasuis-isolates-by-traditional-serotyping-and-molecular-serotyping-methods
#13
Lina Ma, Liyan Wang, Yuefeng Chu, Xuerui Li, Yujun Cui, Shengli Chen, Jianhua Zhou, Chunling Li, Zhongxin Lu, Jixing Liu, Yongsheng Liu
Haemophilus parasuis is classified mainly through serotyping, but traditional serotyping always yields non-typable (NT) strains and unreliable results via cross-reactions. Here, we surveyed the serotype prevalence of Chinese H. parasuis isolates using traditional serotyping (gel immuno-diffusion test, GID) and molecular serotyping (multiplex PCR, mPCR). We also investigated why discrepant results between these methods were obtained, and investigated mPCR failure through whole-genome sequencing. Of the 100 isolate tested, 73 (73%) and 93 (93%) were serotyped by the GID test and mPCR, respectively, with a concordance rate of 66% (66/100)...
2016: PloS One
https://www.readbyqxmd.com/read/27988234/deletion-of-the-vacj-gene-affects-the-biology-and-virulence-in-haemophilus-parasuis-serovar-5
#14
Liangyou Zhao, Xueli Gao, Chaonan Liu, Xiaoping Lv, Nan Jiang, Shimin Zheng
Haemophilus parasuis is an important pathogen causing severe infections in pigs. However, the specific bacterial factors that participate in pathogenic process are poorly understood. VacJ protein is a recently discovered outer membrane lipoprotein that relates to virulence in several pathogens. To characterize the function of the vacJ gene in H. parasuis virulent strain HS49, a vacJ gene-deletion mutant ΔvacJ and its complemented strain were constructed. Our findings supported that VacJ is essential for maintenance of cellular integrity and stress tolerance of H...
March 1, 2017: Gene
https://www.readbyqxmd.com/read/27939158/the-arca-gene-contributes-to-the-serum-resistance-and-virulence-of-haemophilus-parasuis-serovar-13-clinical-strain-ep3
#15
Lingqiang Ding, Xintian Wen, Lvqin He, Xuefeng Yan, Yongping Wen, Sanjie Cao, Xiaobo Huang, Rui Wu, Yiping Wen
As a global transcriptional factor, ArcA regulates the expression of hundreds of genes involved in aerobic and anaerobic metabolism. Here we deleted arcA gene and investigated the biological characteristics of arcA deletion mutant (ΔarcA) in Haemophilus parasuis (H. parasuis) serovar 13 clinical strain EP3. Results indicated that deletion of arcA impaired growth of EP3 strain under anaerobic condition, and reduced virulence of EP3 strain in mice. Additionally, the ΔarcA strain showed greater sensitivity in porcine serum and produced less biofilm mass than the EP3 strain...
November 30, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27939150/haemophilus-parasuis-modulates-cellular-invasion-via-tgf-%C3%AE-1-signaling
#16
Yufeng Li, Yaning Zhang, Yuting Xia, Yijuan Shen, Jiansong Zhang
TGF-β1 plays an important role during the invasion of some bacteria into cells by regulating the expression of integrins, fibronectin, and other extracellular matrix proteins. We postulated that TGF-β1 levels could affect the invasion of Haemophilus parasuis into PK-15 cells. After infection by H. parasuis, PK-15 cells had elevated levels of TGF-β1 expression. Treatment of PK-15 cells with TGF-β1 prior to infection significantly decreased invasion by H. parasuis. Both TGF-β1 treatment and H. parasuis infection resulted in significant induction of fibronectin (Fn) and α5 integrin...
November 30, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27928176/influence-of-parity-of-birth-and-suckled-sows-on-piglet-nasal-mucosal-colonization-with-haemophilus-parasuis
#17
Marion Brean, Sam Abraham, Michelle Hebart, Roy N Kirkwood
Litters of primiparous and multiparous sows were switched at 12 d and nasally swabbed at 12, 18, and 23 d for detection of Haemophilus parasuis. At lactation days 12 and 23, mucosal colonization rates for multiparous and primiparous litters were 0% versus 33% and 26% versus 60%, respectively.
December 2016: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
https://www.readbyqxmd.com/read/27892867/the-balb-c-mouse-infection-model-for-improving-the-haemophilus-parasuis-serotyping-scheme
#18
Jiang Yu, Yuyu Zhang, Yijun Du, Jun Li, Baohua Huang, Wenbo Sun, Xiaoyan Cong, Jun Peng, Sufang Ren, Lihui Gou, Jianli Shi, Zhi Chen, Lei Chen, Jianda Li, Jie Yang, Jiaqiang Wu, Jinbao Wang
The use of BALB/c mouse as an alternative model to study Haemophilus parasuis (HPS) infections was evaluated, supplying the serotyping scheme by comparing the pathogenicity of different serovar HPS in pigs and mice challenge using statistical analysis. Results showed that the pathogenicity of different serovar HPS in mouse was consistent with in pigs, proving that this model is a viable alternative to pigs. It provides a convenient methodology for determining the virulence of HPS strains.
December 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27847372/towards-a-standardized-method-for-broth-microdilution-susceptibility-testing-of-haemophilus-parasuis
#19
Sandra Prüller, Conny Turni, Patrick J Blackall, Martin Beyerbach, Günter Klein, Lothar Kreienbrock, Katrin Strutzberg-Minder, Heike Kaspar, Diana Meemken, Corinna Kehrenberg
Currently, there is no agreed method available for broth microdilution susceptibility testing of Haemophilus parasuis, one of the most important bacterial pathogens in pig production. Therefore, the aim of this study was to develop a method that could be easily performed by diagnostic laboratories and that appears suitable for a harmonized susceptibility testing. Growth determinations using one type strain and three field isolates revealed no visible growth of H. parasuis in media which have proven to be suitable for susceptibility testing of fastidious organisms...
January 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27745687/corrigendum-to-haemophilus-parasuis-infection-activates-chemokine-rantes-in-pk-15-cells-mol-immunol-67-2-part-b-2015-661-666
#20
Yushan Chen, Shanshan Zhou, Kexin Hua, Hongde Xiao, Zili Li, Mei Liu, Rui Luo, Dingren Bi, Rui Zhou, Hui Jin
No abstract text is available yet for this article.
November 2016: Molecular Immunology
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