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Haemophilus parasuis

Lvqin He, Ke Dai, Xintian Wen, Lingqiang Ding, Sanjie Cao, Xiaobo Huang, Rui Wu, Qin Zhao, Yong Huang, Qigui Yan, Xiaoping Ma, Xinfeng Han, Yiping Wen
Haemophilus parasuis is known as a commensal organism discovered in the upper respiratory tract of swine where the pathogenic bacteria survive in various adverse environmental stress. QseC, a histidine protein kinase of the two-component regulatory systems CheY/QseC, is involved in the environmental adaptation in bacteria. To investigate the role of QseC in coping with the adverse environment stresses and survive in the host, we constructed a qseC mutant of H. parasuis serovar 13 strain (Δ qseC ), MY1902. In this study, we found that QseC was involved in stress tolerance of H...
2018: Frontiers in Microbiology
Ke Dai, Lvqin He, Yung-Fu Chang, Sanjie Cao, Qin Zhao, Xiaobo Huang, Rui Wu, Yong Huang, Qigui Yan, Xinfeng Han, Xiaoping Ma, Xintian Wen, Yiping Wen
Haemophilus parasuis causes Glässer's disease and pneumonia, incurring serious economic losses in the porcine industry. In this study, natural competence was investigated in H. parasuis . We found competence genes in H. parasuis homologous to ones in Haemophilus influenzae and a high consensus battery of Sxy-dependent cyclic AMP (cAMP) receptor protein (CRP-S) regulons using bioinformatics. High rates of natural competence were found from the onset of stationary-phase growth condition to mid-stationary phase (OD600 from 0...
2018: Frontiers in Cellular and Infection Microbiology
Zhixin Lei, Qianying Liu, Bing Yang, Saeed Ahmed, Jiyue Cao, Qigai He
The goal of this study was to establish the epidemiological, pharmacodynamic cut-off values, optimal dose regimens for tildipirosin against Haemophilus parasuis. The minimum inhibitory concentrations (MIC) of 164 HPS isolates were determined and SH0165 whose MIC (2 μg/ml ) were selected for PD analysis. The ex vivo MIC in plasma of SH0165 was 0.25 μg/ml which was 8 times lower than that in TSB. The bacteriostatic, bactericidal and elimination activity (AUC24h/MIC) in serum were 26.35, 52.27 and 73.29 h based on the inhibitory sigmoid Emax modeling...
January 5, 2018: Oncotarget
Ke Dai, Xintian Wen, Yung-Fu Chang, Sanjie Cao, Qin Zhao, Xiaobo Huang, Rui Wu, Yong Huang, Qigui Yan, Xinfeng Han, Xiaoping Ma, Yiping Wen
Haemophilus parasuis is a member of the family Pasteurellaceae and a major causative agent of Glässer's disease. This bacterium is normally a benign swine commensal but may become a deadly pathogen upon penetration into multiple tissues, contributing to severe lesions in swine. We have established a successive natural transformation-based markerless mutation system in this species. However, the two-step mutation system requires screening of natural competent cells, and cannot delete genes which regulate natural competence per se...
2018: PeerJ
Shulin Fu, Huashan Liu, Lei Xu, Yinsheng Qiu, Yu Liu, Zhongyuan Wu, Chun Ye, Yongqing Hou, Chien-An Andy Hu
Haemophilus parasuis (H. parasuis) can cause vascular inflammatory injury, but the molecular basis of this effect remains unclear. In this study,we investigated the effect of the anti-inflammatory, anti-microbial and anti-oxidant agent, baicalin, on the nuclear factor (NF)-κB and NLRP3 inflammasome signaling pathway in pig primary aortic vascular endothelial cells. Activation of the NF-κB and NLRP3 inflammasome signaling pathway was induced in H. parasuis-infected cells. However, baicalin reduced the production of reactive oxygen species, apoptosis, and activation of the NF-κB and NLRP3 inflammasome signaling pathway in infected cells...
January 16, 2018: Scientific Reports
Annabelle Mathieu-Denoncourt, Corinne Letendre, Jean-Philippe Auger, Mariela Segura, Virginia Aragon, Sonia Lacouture, Marcelo Gottschalk
Streptococcus suis and Haemophilus parasuis are normal inhabitants of the porcine upper respiratory tract but are also among the most frequent causes of disease in weaned piglets worldwide, causing inflammatory diseases such as septicemia, meningitis and pneumonia. Using an in vitro model of infection with tracheal epithelial cells or primary alveolar macrophages (PAMs), it was possible to determine the interaction between S. suis serotype 2 and H. parasuis strains with different level of virulence. Within H...
January 6, 2018: Pathogens
Yongping Wen, Yiping Wen, Xintian Wen, Sanjie Cao, Xiaobo Huang, Rui Wu, Qin Zhao, Mafeng Liu, Yong Huang, Qigui Yan, Xinfeng Han, Xiaoping Ma, Ke Dai, Lingqiang Ding, Sitong Liu, Jian Yang
Haemophilus parasuis is an opportunistic pathogen and the causative agent of Glässer's disease in swine. This disease has high morbidity and mortality rates in swine populations, and is responsible for major economic losses worldwide. Survival of H. parasuis within the host requires mechanisms for coping with oxidative stress conditions. In many bacteria, OxyR is known to mediate protection against oxidative stress; however, little is known about the role of OxyR in H. parasuis. In the current study, an oxyR mutant strain was constructed in H...
February 15, 2018: Gene
Małgorzata Pomorska-Mól, Arkadiusz Dors, Krzysztof Kwit, Ewelina Czyżewska-Dors, Zygmunt Pejsak
BACKGROUND: Respiratory co-infections are important factor affecting the profitability of pigs production. Swine influenza virus (SIV) may predispose to secondary infection. Haemophilus parasuis (Hps) can be a primary pathogen or be associated with other pathogens such as SIV. To date, little is known about the effect of coinfection with SIV and Hps on the disease severity and inflammatory response and the role of Hps in the induction of pneumonia in the absence of other respiratory pathogens...
December 4, 2017: BMC Veterinary Research
Shuqing Liu, Wentao Li, Yang Wang, Changqin Gu, Xiaoli Liu, Catherine Charreyre, Shenxian Fan, Qigai He
BACKGROUND: Postweaning multisystemic wasting syndrome (PMWS) is an emerging disease in swine. Pigs with PMWS are often infected with a variety of other pathogens, including bacteria, viruses and mycoplasm, in addition to porcine circovirus type 2 (PCV2). PCV2 and Haemophilus parasuis serovar 4 (HPS4) coinfection remain epidemic in China. METHODS: Here we report construction of a three-week-old naturally farrowed, colostrum-deprived (NFCD) piglet's infection model and demonstrate that PCV2-infected piglets with the HPS4 coinfection increased the virulence of PCV2 and these pathogens interact acquired PMWS...
November 21, 2017: Virology Journal
Qi Cao, Fenfen Feng, Huan Wang, Xiaojuan Xu, Huanchun Chen, Xuwang Cai, Xiangru Wang
Haemophilus parasuis is an opportunistic pathogen localized in the upper respiratory tracts of pigs, its infection begins from bacterial survival under complex conditions, like hyperosmosis, oxidative stress, phagocytosis, and sometimes antibiotics as well. The two-component signal transduction (TCST) system serves as a common stimulus-response mechanism that allows microbes to sense and respond to diverse environmental conditions via a series of phosphorylation reactions. In this study, we investigated the role of TCST system CpxRA in H...
January 2018: Microbiological Research
Z Ling, L Yonghong, L Junfeng, Z Li, L Xianqiang
The combined antibacterial effects of tilmicosin (TIL) and florfenicol (FF) against Actinobacillus pleuropneumoniae (APP) (n = 2), Streptococcus suis (S. suis) (n = 2), and Haemophilus parasuis (HPS) (n = 2) were evaluated by chekerboard test and time-kill assays. The pharmacokinetics (PKs) of TIL- and FF-loaded hydrogenated castor oil (HCO)-solid lipid nanoparticles (SLN) were performed in healthy pigs. The results indicated that TIL and FF showed synergistic or additive antibacterial activities against APP, S...
November 15, 2017: Journal of Veterinary Pharmacology and Therapeutics
Hongchao Gou, Juan Li, Rujian Cai, Shuai Song, Miao Li, Dongxia Yang, Zhiyong Jiang, Yan Li, Pinpin Chu, Chunling Li
Haemophilus parasuis infection is of considerable economic importance in the swine industry due to high morbidity and mortality in naive swine populations. Accurate detection and identification of the causative agent are difficult, yet necessary, for disease control. In this study, a simple and rapid method of cross-priming amplification (CPA) with a vertical flow (VF) visualization strip was established to detect H. parasuis. The reaction can specifically identify 15 serovar reference strains and 57 clinically isolated strains of H...
January 2018: Journal of Microbiological Methods
Bin Ma, Kexin Hua, Shanshan Zhou, Hufeng Zhou, Yushan Chen, Rui Luo, Dingren Bi, Rui Zhou, Qigai He, Hui Jin
Haemophilus parasuis, an important swine pathogen, was recently proven able to invade into endothelial or epithelial cell in vitro. NOD1/2 are specialized NLRs that participate in the recognition of pathogens able to invade intracellularly and therefore, we assessed that the contribution of NOD1/2 to inflammation responses during H. parasuis infection. We observed that H. parasuis infection enhanced NOD2 expression and RIP2 phosphorylation in porcine kidney 15 cells. Our results also showed that knock down of NOD1/2 or RIP2 expression respectively significantly decreased H...
October 31, 2017: Developmental and Comparative Immunology
Christiane Weissenbacher-Lang, Nora Nedorost, Christian Knecht, Isabel Hennig-Pauka, Mathias Huber, Thomas Voglmayr, Herbert Weissenböck
Pneumocystis carinii f. sp. suis (PCS) nucleic acid and antibody profiles on two Austrian-farrow-to-finish farms were investigated. Furthermore, associations with other respiratory pathogens were evaluated. Respiratory specimen and sera from pigs of five age classes between the 1st week and the 3rd month of life as well as samples from sows were analyzed. On Farm A, PCS infection occurred early in life. The suckling piglets were already infected in the 1st week of life and the pigs remained positive until the 3rd month of life...
2017: PloS One
Aiqing Jia, Ruyue Zhou, Huiying Fan, Kaijie Yang, Jianmin Zhang, Yindi Xu, Guiping Wang, Ming Liao
The bacterium Haemophilus parasuis is the specific pathogenic cause of Glässer's disease in swine. Fifteen serotypes of H. parasuis have been reported. A method to serotype H. parasuis isolates accurately would help to prevent and control Glässer's disease outbreaks through appropriate vaccination and to understand the epidemiology in specific geographic areas. However, according to traditional serotyping, the rate of nontypeable (NT) strains is 10 to 40%, which gives low accuracy. In the present study, we developed a set of PCR assays that are able to identify all the currently known H...
November 2017: Journal of Clinical Microbiology
Bibiana Martins Barasuol, João Antônio Guizzo, Jamie Elisabeth Fegan, Sonia Martínez-Martínez, Elías Fernando Rodríguez-Ferri, César Bernardo Gutiérrez-Martín, Luiz Carlos Kreutz, Anthony Bernard Schryvers, Rafael Frandoloso
Vaccines have become fundamental in the control and elimination of Glässer Disease, a systemic disease of pigs caused by Haemophilus parasuis. The classic vaccines available for prevention of this infection were developed without a robust knowledge about host immunological mechanisms. In this study, we demonstrated the presence of cross-reactive epitopes on both the N-lobe and C-lobe of variants of transferrin binding protein B (TbpBs) expressed on the surface of 6 virulent serovars of H. parasuis. Antibodies against TbpB-derived antigens were capable of increasing the phagocytic capacity of neutrophils and were also capable of blocking porcine transferrin from binding to TbpB...
September 4, 2017: Scientific Reports
Zhixin Lei, Shulin Fu, Bing Yang, Qianying Liu, Saeed Ahmed, Lei Xu, Jincheng Xiong, Jiyue Cao, Yinsheng Qiu
Numerous studies have been conducted to examine the molecular mechanism of Haemophilus parasuis resistance to antibiotic, but rarely to tildipirosin. In the current study, transcriptional profiling was applied to analyse the variation in gene expression of JS0135 and tildipirosin-resistant JS32. The growth curves showed that JS32 had a higher growth rate but fewer bacteria than JS0135. The cell membranes of JS32 and a resistant clinical isolate (HB32) were observed to be smoother than those of JS0135. From the comparative gene expression profile 349 up- and 113 downregulated genes were observed, covering 37 GO and 63 KEGG pathways which are involved in biological processes (11), cellular components (17), molecular function (9), cellular processes (1), environmental information processing (4), genetic information processing (9) and metabolism (49) affected in JS32...
August 8, 2017: Scientific Reports
Sonia Lacouture, Edisleidy Rodriguez, Katrin Strutzberg-Minder, Marcelo Gottschalk
No abstract text is available yet for this article.
August 2017: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
Ze Zeng, Bin Zhang, Huan He, Xinnuo Chen, Yupeng Ren, Hua Yue, Cheng Tang
The lgtF gene encodes a glucosyltransferase responsible for adding a glucose to the first sugar of heptose I in the synthesis of lipooligosaccharides (LOS). To study the function of lgtF, we constructed an lgtF mutant (ΔlgtF) from Haemophilus parasuis SC096 using a natural transformation system. A highly purified preparation of LOS from ΔlgtF (ΔlgtF-LOS) exhibited an obvious truncation in structure compared to the LOS of the wild-type SC096 strain (WT-LOS). The ΔlgtF-LOS also displayed a significantly reduced ability to induce inflammatory cytokine mRNA expression of tumor necrosis factor alpha (TNF-α), interleukin-1α (IL-1α), IL-1β, IL-6 and IL-8 in porcine alveolar macrophages (PAMs) in comparison with the WT-LOS...
July 14, 2017: Microbial Pathogenesis
Li Li, Ye Tian, Jiankang Yu, Xu Song, Renyong Jia, Qiankun Cui, Wenzhi Tong, Yuanfeng Zou, Lixia Li, Lizi Yin, Xiaoxia Liang, Changliang He, Guizhou Yue, Gang Ye, Ling Zhao, Fei Shi, Cheng Lv, Sanjie Cao, Zhongqiong Yin
Haemophilus parasuis, a symbiotic bacteria of upper respiratory tract of swine, is the etiological agent of Glässer's disease, which is characterized by fibrinous polyserositis. Emodin, exhibits antibacterial activity against H. parasuis, yet the action mode has not been fully understood. In present study, isobaric tag for relative and absolute quantification (iTRAQ) method was applied to analyze the global protein alteration of H. parasuis in response to 16μg/mL Emodin. In total, 338 proteins exhibiting significant differential expressions were identified...
July 2, 2017: Journal of Proteomics
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