keyword
MENU ▼
Read by QxMD icon Read
search

Haemophilus parasuis

keyword
https://www.readbyqxmd.com/read/29146255/haemophilus-parasuis-cpxra-two-component-system-confers-bacterial-tolerance-to-environmental-stresses-and-macrolide-resistance
#1
Qi Cao, Fenfen Feng, Huan Wang, Xiaojuan Xu, Huanchun Chen, Xuwang Cai, Xiangru Wang
Haemophilus parasuis is an opportunistic pathogen localized in the upper respiratory tracts of pigs, its infection begins from bacterial survival under complex conditions, like hyperosmosis, oxidative stress, phagocytosis, and sometimes antibiotics as well. The two-component signal transduction (TCST) system serves as a common stimulus-response mechanism that allows microbes to sense and respond to diverse environmental conditions via a series of phosphorylation reactions. In this study, we investigated the role of TCST system CpxRA in H...
January 2018: Microbiological Research
https://www.readbyqxmd.com/read/29139136/tilmicosin-and-florfenicol-loaded-hydrogenated-castor-oil-solid-lipid-nanoparticles-to-pigs-combined-antibacterial-activities-and-pharmacokinetics
#2
Z Ling, L Yonghong, L Junfeng, Z Li, L Xianqiang
The combined antibacterial effects of tilmicosin (TIL) and florfenicol (FF) against Actinobacillus pleuropneumoniae (APP) (n = 2), Streptococcus suis (S. suis) (n = 2), and Haemophilus parasuis (HPS) (n = 2) were evaluated by chekerboard test and time-kill assays. The pharmacokinetics (PKs) of TIL- and FF-loaded hydrogenated castor oil (HCO)-solid lipid nanoparticles (SLN) were performed in healthy pigs. The results indicated that TIL and FF showed synergistic or additive antibacterial activities against APP, S...
November 15, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29128480/rapid-detection-of-haemophilus-parasuis-using-cross-priming-amplification-and-vertical-flow-visualization
#3
Hongchao Gou, Juan Li, Rujian Cai, Shuai Song, Miao Li, Dongxia Yang, Zhiyong Jiang, Yan Li, Pinpin Chu, Chunling Li
Haemophilus parasuis infection is of considerable economic importance in the swine industry due to high morbidity and mortality in naive swine populations. Accurate detection and identification of the causative agent are difficult, yet necessary, for disease control. In this study, a simple and rapid method of cross-priming amplification (CPA) with a vertical flow (VF) visualization strip was established to detect H. parasuis. The reaction can specifically identify 15 serovar reference strains and 57 clinically isolated strains of H...
November 9, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29097236/haemophilus-parasuis-infection-activates-nod1-2-rip2-signaling-pathway-in-pk-15%C3%A2-cells
#4
Bin Ma, Kexin Hua, Shanshan Zhou, Hufeng Zhou, Yushan Chen, Rui Luo, Dingren Bi, Rui Zhou, Qigai He, Hui Jin
Haemophilus parasuis, an important swine pathogen, was recently proven able to invade into endothelial or epithelial cell in vitro. NOD1/2 are specialized NLRs that participate in the recognition of pathogens able to invade intracellularly and therefore, we assessed that the contribution of NOD1/2 to inflammation responses during H. parasuis infection. We observed that H. parasuis infection enhanced NOD2 expression and RIP2 phosphorylation in porcine kidney 15 cells. Our results also showed that knock down of NOD1/2 or RIP2 expression respectively significantly decreased H...
October 31, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28945819/comparison-of-pneumocystis-nucleic-acid-and-antibody-profiles-and-their-associations-with-other-respiratory-pathogens-in-two-austrian-pig-herds
#5
COMPARATIVE STUDY
Christiane Weissenbacher-Lang, Nora Nedorost, Christian Knecht, Isabel Hennig-Pauka, Mathias Huber, Thomas Voglmayr, Herbert Weissenböck
Pneumocystis carinii f. sp. suis (PCS) nucleic acid and antibody profiles on two Austrian-farrow-to-finish farms were investigated. Furthermore, associations with other respiratory pathogens were evaluated. Respiratory specimen and sera from pigs of five age classes between the 1st week and the 3rd month of life as well as samples from sows were analyzed. On Farm A, PCS infection occurred early in life. The suckling piglets were already infected in the 1st week of life and the pigs remained positive until the 3rd month of life...
2017: PloS One
https://www.readbyqxmd.com/read/28878007/development-of-serotype-specific-pcr-assays-for-typing-of-haemophilus-parasuis-isolates-circulating-in-southern-china
#6
Aiqing Jia, Ruyue Zhou, Huiying Fan, Kaijie Yang, Jianmin Zhang, Yindi Xu, Guiping Wang, Ming Liao
The bacterium Haemophilus parasuis is the specific pathogenic cause of Glässer's disease in swine. Fifteen serotypes of H. parasuis have been reported. A method to serotype H. parasuis isolates accurately would help to prevent and control Glässer's disease outbreaks through appropriate vaccination and to understand the epidemiology in specific geographic areas. However, according to traditional serotyping, the rate of nontypeable (NT) strains is 10 to 40%, which gives low accuracy. In the present study, we developed a set of PCR assays that are able to identify all the currently known H...
November 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28871190/new-insights-about-functional-and-cross-reactive-properties-of-antibodies-generated-against-recombinant-tbpbs-of-haemophilus-parasuis
#7
Bibiana Martins Barasuol, João Antônio Guizzo, Jamie Elisabeth Fegan, Sonia Martínez-Martínez, Elías Fernando Rodríguez-Ferri, César Bernardo Gutiérrez-Martín, Luiz Carlos Kreutz, Anthony Bernard Schryvers, Rafael Frandoloso
Vaccines have become fundamental in the control and elimination of Glässer Disease, a systemic disease of pigs caused by Haemophilus parasuis. The classic vaccines available for prevention of this infection were developed without a robust knowledge about host immunological mechanisms. In this study, we demonstrated the presence of cross-reactive epitopes on both the N-lobe and C-lobe of variants of transferrin binding protein B (TbpBs) expressed on the surface of 6 virulent serovars of H. parasuis. Antibodies against TbpB-derived antigens were capable of increasing the phagocytic capacity of neutrophils and were also capable of blocking porcine transferrin from binding to TbpB...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28790420/comparative-transcriptional-profiling-of-tildipirosin-resistant-and-sensitive-haemophilus-parasuis
#8
Zhixin Lei, Shulin Fu, Bing Yang, Qianying Liu, Saeed Ahmed, Lei Xu, Jincheng Xiong, Jiyue Cao, Yinsheng Qiu
Numerous studies have been conducted to examine the molecular mechanism of Haemophilus parasuis resistance to antibiotic, but rarely to tildipirosin. In the current study, transcriptional profiling was applied to analyse the variation in gene expression of JS0135 and tildipirosin-resistant JS32. The growth curves showed that JS32 had a higher growth rate but fewer bacteria than JS0135. The cell membranes of JS32 and a resistant clinical isolate (HB32) were observed to be smoother than those of JS0135. From the comparative gene expression profile 349 up- and 113 downregulated genes were observed, covering 37 GO and 63 KEGG pathways which are involved in biological processes (11), cellular components (17), molecular function (9), cellular processes (1), environmental information processing (4), genetic information processing (9) and metabolism (49) affected in JS32...
August 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28761183/canada-serotyping-of-haemophilus-parasuis-field-isolates-from-diseased-pigs-in-quebec-by-indirect-hemagglutination-assay-and-multiplex-polymerase-chain-reaction-pcr
#9
Sonia Lacouture, Edisleidy Rodriguez, Katrin Strutzberg-Minder, Marcelo Gottschalk
No abstract text is available yet for this article.
August 2017: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
https://www.readbyqxmd.com/read/28716662/lgtf-effects-of-haemophilus-parasuis-los-induced-inflammation-through-regulation-of-nf-%C3%AE%C2%BAb-and-mapks-signaling-pathways
#10
Ze Zeng, Bin Zhang, Huan He, Xinnuo Chen, Yupeng Ren, Hua Yue, Cheng Tang
The lgtF gene encodes a glucosyltransferase responsible for adding a glucose to the first sugar of heptose I in the synthesis of lipooligosaccharides (LOS). To study the function of lgtF, we constructed an lgtF mutant (ΔlgtF) from Haemophilus parasuis SC096 using a natural transformation system. A highly purified preparation of LOS from ΔlgtF (ΔlgtF-LOS) exhibited an obvious truncation in structure compared to the LOS of the wild-type SC096 strain (WT-LOS). The ΔlgtF-LOS also displayed a significantly reduced ability to induce inflammatory cytokine mRNA expression of tumor necrosis factor alpha (TNF-α), interleukin-1α (IL-1α), IL-1β, IL-6 and IL-8 in porcine alveolar macrophages (PAMs) in comparison with the WT-LOS...
July 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28679110/itraq-based-quantitative-proteomic-analysis-reveals-multiple-effects-of-emodin-to-haemophilus-parasuis
#11
Li Li, Ye Tian, Jiankang Yu, Xu Song, Renyong Jia, Qiankun Cui, Wenzhi Tong, Yuanfeng Zou, Lixia Li, Lizi Yin, Xiaoxia Liang, Changliang He, Guizhou Yue, Gang Ye, Ling Zhao, Fei Shi, Cheng Lv, Sanjie Cao, Zhongqiong Yin
Haemophilus parasuis, a symbiotic bacteria of upper respiratory tract of swine, is the etiological agent of Glässer's disease, which is characterized by fibrinous polyserositis. Emodin, exhibits antibacterial activity against H. parasuis, yet the action mode has not been fully understood. In present study, isobaric tag for relative and absolute quantification (iTRAQ) method was applied to analyze the global protein alteration of H. parasuis in response to 16μg/mL Emodin. In total, 338 proteins exhibiting significant differential expressions were identified...
July 2, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28619168/comparison-of-asian-porcine-high-fever-disease-isolates-of-porcine-reproductive-and-respiratory-syndrome-virus-to-united-states-isolates-for-their-ability-to-cause-disease-and-secondary-bacterial-infection-in-swine
#12
Susan L Brockmeier, Crystal L Loving, Mitchel V Palmer, Allyn Spear, Tracy L Nicholson, Kay S Faaberg, Kelly M Lager
Epidemiologic data from Asian outbreaks of highly-pathogenic (HP) porcine reproductive and respiratory syndrome virus (PRRSV) suggest that disease severity was associated with both the virulence of the PRRSV isolates and secondary bacterial infections. Previous reports have indicated that U.S. isolates of PRRSV predispose to secondary bacterial infections as well, but the severity of disease that occurred in Asia in pigs infected with these HP-PRRSV strains has not been reported in the U.S. The objectives of this research were to compare the pathogenesis of Asian and U...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28615466/-pathotyping-multiplex-pcr-assay-for-haemophilus-parasuis-a-tool-for-prediction-of-virulence
#13
Kate J Howell, Lucy A Weinert, Sarah E Peters, Jinhong Wang, Juan Hernandez-Garcia, Roy R Chaudhuri, Shi-Lu Luan, Øystein Angen, Virginia Aragon, Susanna M Williamson, Paul R Langford, Andrew N Rycroft, Brendan W Wren, Duncan J Maskell, Alexander W Tucker
Haemophilus parasuis is a diverse bacterial species that is found in the upper respiratory tracts of pigs and can also cause Glässer's disease and pneumonia. A previous pangenome study of H. parasuis identified 48 genes that were associated with clinical disease. Here, we describe the development of a generalized linear model (termed a pathotyping model) to predict the potential virulence of isolates of H. parasuis based on a subset of 10 genes from the pangenome. A multiplex PCR (mPCR) was constructed based on these genes, the results of which were entered into the pathotyping model to yield a prediction of virulence...
September 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28584712/prevalence-and-seroepidemiology-of-haemophilus-parasuis-in-sichuan-province-china
#14
Zhenghao Wang, Qin Zhao, Hailin Wei, Xintian Wen, Sanjie Cao, Xiaobo Huang, Rui Wu, Qigui Yan, Yong Huang, Yiping Wen
Haemophilus parasuis, the causative agent of Glässer's disease, has been reported as widespread, but little is known about its epidemiology in the Sichuan province of China. The goal of our research is to reveal the prevalence and distribution of H. parasuis in this area. Sampling and isolation were performed across Sichuan; isolates were processed using serotyping multiplex PCR (serotyping-mPCR) and agar gel diffusion (AGD) for confirmation of serovar identity. This study was carried out from January 2014 to May 2016 and 254 H...
2017: PeerJ
https://www.readbyqxmd.com/read/28545143/haemophilus-parasuis-cytolethal-distending-toxin-induces-cell-cycle-arrest-and-p53-dependent-apoptosis
#15
Gang Li, Hui Niu, Yanhe Zhang, Yanling Li, Fang Xie, Paul R Langford, Siguo Liu, Chunlai Wang
Haemophilus parasuis is the causative agent of Glasser's disease in pigs. Cytolethal distending toxin (CDT) is an important virulence factor of H. parasuis. It is composed of three subunits: CdtA, CdtB and CdtC and all were successfully expressed in soluble form in Escherichia coli when the signal peptides were removed. Purified CdtB had DNase activity, i.e. caused DNA double strand damage, in vitro and in vivo prior to cell arrest and apoptosis. Flow cytometry analysis showed CdtB alone could induce cell cycle arrest and apoptosis in PK-15 porcine kidney and pulmonary alveolar macrophage (PAM) cells, which could be enhanced by CdtA or/and CdtC...
2017: PloS One
https://www.readbyqxmd.com/read/28532803/secondary-haemophilus-parasuis-infection-enhances-highly-pathogenic-porcine-reproductive-and-respiratory-syndrome-virus-hp-prrsv-infection-mediated-inflammatory-responses
#16
Jiangnan Li, Shengnan Wang, Changyao Li, Chunlai Wang, Yonggang Liu, Gang Wang, Xijun He, Liang Hu, Yuanyuan Liu, Mengmeng Cui, Caihong Bi, Zengyu Shao, Xiaojie Wang, Tao Xiong, Xuehui Cai, Li Huang, Changjiang Weng
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection often predisposes pigs to secondary bacterial infection, which induces robust inflammatory responses. However, whether the secondary bacterial infection synergizes HP-PRRSV infection and enhances inflammatory responses is not fully understood. Here, we characterized HP-PRRSV infection-mediated secondary bacterial infection and robust inflammatory responses. HP-PRRSV infection induced higher levels of cytokines (IL-1β, IL-18, IL-6 and TNF-α) in the sera in piglets and bacterial loads of 11 bacterial species in the lung were increased after HP-PRRSV infection, including Mycoplasma hyorhinis, Haemophilus parasuis and Escherichia coli...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28528009/identification-of-novel-haemophilus-parasuis-serovar-5-vaccine-candidates-using-an-immunoproteomic-approach
#17
Gang Li, Fang Xie, Jianjun Li, Jiao Liu, Dapeng Li, Yanhe Zhang, Paul R Langford, Yanwen Li, Siguo Liu, Chunlai Wang
Haemophilus parasuis is the aetiological agent of Glässer's disease, which is responsible for cases of fibrinous polyserositis, polyarthritis and meningitis. No vaccine is known that provides cross-protection against all serovars. The identification of novel immunoprotective antigens would undoubtedly contribute to the development of efficient subunit vaccines. In the present study, an immunoproteomic approach was used to analyze secreted proteins of H. parasuis and six proteins with high immunogenicity were identified...
May 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28482900/a-robust-pcr-for-the-differentiation-of-potential-virulent-strains-of-haemophilus-parasuis
#18
N Galofré-Milà, F Correa-Fiz, S Lacouture, M Gottschalk, K Strutzberg-Minder, A Bensaid, S Pina-Pedrero, V Aragon
BACKGROUND: Haemophilus parasuis is the etiological agent of Glässer's disease in swine. H. parasuis comprises strains with heterogeneous virulence capacity, from non-virulent to highly virulent. Determination of the pathogenic potential of the strains is important for diagnosis and disease control. The virulence-associated trimeric autotransporters (vtaA) genes have been used to predict H. parasuis virulence by PCR amplification of their translocator domains. Here, we report a new and improved PCR designed to detect a different domain of the vtaA genes, the leader sequence (LS) as a diagnostic tool to predict virulence...
May 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28472979/concurrent-infection-with-porcine-reproductive-and-respiratory-syndrome-virus-and-haemophilus-parasuis-in-two-types-of-porcine-macrophages-apoptosis-production-of-ros-and-formation-of-multinucleated-giant-cells
#19
Lenka Kavanová, Katarína Matiašková, Lenka Levá, Hana Štěpánová, Kateřina Nedbalcová, Ján Matiašovic, Martin Faldyna, Jiří Salát
Porcine reproductive and respiratory syndrome (PRRS) is one of the most significant and economically important infectious diseases affecting swine worldwide and can predispose pigs to secondary bacterial infections caused by, e.g. Haemophilus parasuis. The aim of the presented study was to compare susceptibility of two different types of macrophages which could be in contact with both pathogens during infection with PRRS virus (PRRSV) and in co-infection with H. parasuis. Alveolar macrophages (PAMs) as resident cells provide one of the first lines of defence against microbes invading lung tissue...
May 4, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28448603/evaluation-of-immunogenicity-and-protective-efficacy-of-recombinant-outer-membrane-proteins-of-haemophilus-parasuis-serovar-5-in-a-murine-model
#20
Miao Li, Ru-Jian Cai, Shuai Song, Zhi-Yong Jiang, Yan Li, Hong-Chao Gou, Pin-Pin Chu, Chun-Ling Li, Hua-Ji Qiu
Glässer's disease is an economically important infectious disease of pigs caused by Haemophilus parasuis. Few vaccines are currently available that could provide effective cross-protection against various serovars of H. parasuis. In this study, five OMPs (OppA, TolC, HxuC, LppC, and HAPS_0926) identified by bioinformatic approaches, were cloned and expressed as recombinant proteins. Antigenicity of the purified proteins was verified through Western blotting, and primary screening for protective potential was evaluated in vivo...
2017: PloS One
keyword
keyword
5402
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"