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Haemophilus parasuis

Hiroki Shinkai, Kei Terada, Daisuke Toki, Masanori Tohno, Hirohide Uenishi
Nucleotide-binding domain, leucine-rich-containing family, pyrin-domain containing-3 (NLRP3) is an important pattern recognition receptor involved in various inflammatory responses and adjuvant effects upon vaccination. We previously identified the Q969R (A2906G) gain-of-function polymorphism in porcine NLRP3, which increased production of interleukin-1β in in vitro gene transfection experiments. Here, we explored the associations between the A2906G polymorphism and antibody responses after vaccination against bacteria in Large White pigs maintained under specific pathogen-free conditions...
May 17, 2018: Animal Science Journal, Nihon Chikusan Gakkaihō
João Antônio Guizzo, Somshukla Chaudhuri, Simone Ramos Prigol, Rong-Hua Yu, Cláudia Cerutti Dazzi, Natalia Balbinott, Gabriela Paraboni Frandoloso, Luiz Carlos Kreutz, Rafael Frandoloso, Anthony Bernard Schryvers
Haemophilus parasuis is the causative agent of the Glässer's disease (GD), one of the most important bacterial diseases that affect young pigs worldwide. GD prevention based on vaccination is a major concern due to the limited cross-protection conferred by the inactivated whole cell vaccines used currently. In this study, vaccines based on two mutant recombinant proteins derived from transferrin binding protein B of H. parasuis (Y167A-TbpB and W176A-TbpB) were formulated and evaluated in terms of protection against lethal challenge using a serovar 7 (SV7) H...
May 9, 2018: Scientific Reports
Shulin Fu, Jing Guo, Ruizhi Li, Yinsheng Qiu, Chun Ye, Yu Liu, Zhongyuan Wu, Ling Guo, Yongqing Hou, Chien-An Andy Hu
Haemophilus parasuis is the causative agent of Glässer’s disease in pigs. H. parasuis can cause vascular damage, although the mechanism remains unclear. In this study, we investigated the host cell responses involved in the molecular pathway interactions in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis using RNA-Seq. The transcriptome results showed that when PAVECs were infected with H. parasuis for 24 h, 281 differentially expressed genes (DEGs) were identified; of which, 236 were upregulated and 45 downregulated...
April 29, 2018: International Journal of Molecular Sciences
Shulin Fu, Huashan Liu, Xiao Chen, Yinsheng Qiu, Chun Ye, Yu Liu, Zhongyuan Wu, Ling Guo, Yongqing Hou, Chien-An Andy Hu
Haemophilus parasuis ( H. parasuis ) can cause Glässer’s disease in pigs. However, the molecular mechanism of the inflammation response induced by H. parasuis remains unclear. The high-mobility group box 1 (HMGB1) protein is related to the pathogenesis of various infectious pathogens, but little is known about whether H. parasuis can induce the release of HMGB1 in piglet peripheral blood monocytes. Baicalin displays important anti-inflammatory and anti-microbial activities. In the present study, we investigated whether H...
April 27, 2018: International Journal of Molecular Sciences
L Zhang, Y Li, Y Wang, A Sajid, S Ahmed, X Li
The aims of this study were to establish optimal doses of doxycycline (dox) against Haemophilus parasuis on the basis of pharmacokinetic-pharmacodynamic (PK-PD) integration modeling. The infected model was established by intranasal inoculation of organism in pigs and confirmed by clinical signs, blood biochemistry, and microscopic examinations. The recommended dose (20 mg/kg b.w.) was administered in pigs through intramuscular routes for PK studies. The area under the concentration 0- to 24-hr curve (AUC0-24 ), elimination half-life (T½ke ), and mean residence time (MRT) of dox in healthy and H...
April 25, 2018: Journal of Veterinary Pharmacology and Therapeutics
J S Zhang, Y T Xia, R C Zheng, Z Y Liang, Y J Shen, Y F Li, M Nie, C Gu, H Wang
Thirty clinical isolates of H. parasuis from pig farms in eastern China were screened for antimicrobial susceptibility. A novel plasmid, designated pHPSGC, was extracted from one isolate with evidence of resistance (elevated minimum inhibitory concentration) to florfenicol. DNA sequencing demonstrated that pHPSGC (5297 base pairs) contains three open reading frames (ORFs), corresponding to the genes rep, floR and lysR. The rep gene of pHPSGC shared 99% sequence identity with the rep gene of pHPS1019. In addition, the region containing floR and lysR in pHPSGC shared 99% similarity with the corresponding region of pCCK381...
April 2018: Veterinary Journal
Yongda Zhao, Lili Guo, Jie Li, Xianhui Huang, Binghu Fang
Background: Haemophilus parasuis is a common porcine respiratory pathogen that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored by H. parasuis from pig farms in China. Methods: We screened 143 H. parasuis isolates for antimicrobial susceptibility against six fluoroquinolone antibiotics testing by the broth microdilution method, and the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis...
2018: PeerJ
Ze Zeng, Xinnuo Chen, Hua Yue, Huan He, Yupeng Ren, Cheng Tang, Bin Zhang
In Haemophilus parasuis, the rfa cluster has been identified as a virulence-associated factor that is involved in lipooligosaccharide (LOS) biosynthesis. In this study, we assessed the roles of rfaD and rfaF genesin H. parasuis SC096 on LOS-induced pro-inflammatory factors and the related signaling pathways in porcine alveolar macrophages (PAMs) by real-time PCR and western blotting. The results showed that the LOSs of both rfaD and rfaF mutants (ΔrfaD-LOS and ΔrfaF-LOS) significantly decreased the mRNA expression of pro-inflammatory factors (IL-1α, IL-1β, IL-6, IL-8 and TNF-α) in PAMs compared with H...
April 6, 2018: Journal of Veterinary Medical Science
Sandra Brogden, Andrea Pavlović, Regina Tegeler, Heike Kaspar, Nicole De Vaan, Corinna Kehrenberg
Haemophilus parasuis-related infections, especially among weaners are responsible for major economic losses on pig farms. A method for broth microdilution susceptibility testing of this fastidious organism has recently been developed, but the suitability of this method needs to be validated in a large collection of current field isolates. Using the proposed method, this study tested 123 H. parasuis isolates from different geographic regions in Germany (including five isolates from the Netherlands and Belgium) against a panel of 24 antimicrobial agents and antimicrobial combinations...
April 2018: Veterinary Microbiology
Annet Heuvelink, Jeanine Wiegel, Corinna Kehrenberg, Remco Dijkman, Edgardo Soriano-Vargas, Anneke Feberwee
The objective of the present study was to determine the in vitro antimicrobial susceptibility of Avibacterium paragallinarum isolates from infectious coryza outbreaks in Dutch commercial poultry, from 2008 till mid-2017. By using a broth microdilution method, minimal inhibitory concentrations (MICs) of 15 antimicrobial agents were assessed, and MIC50 and MIC90 values were determined. Additionally, isolates were subjected to different PCRs for the presence of genes that may confer antimicrobial resistance. Besides field isolates, a set of reference strains, among which the nine Kume strains and one Page serovar strain, were included in the study...
April 2018: Veterinary Microbiology
Anne E Watt, Glenn F Browning, Alistair R Legione, Rhys N Bushell, Andrew Stent, Ross S Cutler, Neil D Young, Marc S Marenda
An unknown member of the family Pasteurellaceae was repeatedly isolated from severe pulmonary lesions in 20-24 week old pigs reared on the same farm in Victoria, Australia. The aetiological diagnosis of the disease was inconclusive. The complete genome sequence analysis of one strain, 15-184, revealed some phylogenic proximity to Glaesserella (Haemophilus) parasuis , the cause of Glasser's disease. However, sequences of the 16S rRNA and housekeeping genes, as well as average nucleotide identity scores, differed from all other known species in the family Pasteurellaceae The protein content of 15-184 was composite, with 60% of coding sequences matching known G...
March 23, 2018: Applied and Environmental Microbiology
Lvqin He, Ke Dai, Xintian Wen, Lingqiang Ding, Sanjie Cao, Xiaobo Huang, Rui Wu, Qin Zhao, Yong Huang, Qigui Yan, Xiaoping Ma, Xinfeng Han, Yiping Wen
Haemophilus parasuis is known as a commensal organism discovered in the upper respiratory tract of swine where the pathogenic bacteria survive in various adverse environmental stress. QseC, a histidine protein kinase of the two-component regulatory systems CheY/QseC, is involved in the environmental adaptation in bacteria. To investigate the role of QseC in coping with the adverse environment stresses and survive in the host, we constructed a qseC mutant of H. parasuis serovar 13 strain (Δ qseC ), MY1902. In this study, we found that QseC was involved in stress tolerance of H...
2018: Frontiers in Microbiology
Ke Dai, Lvqin He, Yung-Fu Chang, Sanjie Cao, Qin Zhao, Xiaobo Huang, Rui Wu, Yong Huang, Qigui Yan, Xinfeng Han, Xiaoping Ma, Xintian Wen, Yiping Wen
Haemophilus parasuis causes Glässer's disease and pneumonia, incurring serious economic losses in the porcine industry. In this study, natural competence was investigated in H. parasuis . We found competence genes in H. parasuis homologous to ones in Haemophilus influenzae and a high consensus battery of Sxy-dependent cyclic AMP (cAMP) receptor protein (CRP-S) regulons using bioinformatics. High rates of natural competence were found from the onset of stationary-phase growth condition to mid-stationary phase (OD600 from 0...
2018: Frontiers in Cellular and Infection Microbiology
Zhixin Lei, Qianying Liu, Bing Yang, Saeed Ahmed, Jiyue Cao, Qigai He
The goal of this study was to establish the epidemiological, pharmacodynamic cut-off values, optimal dose regimens for tildipirosin against Haemophilus parasuis . The minimum inhibitory concentrations (MIC) of 164 HPS isolates were determined and SH0165 whose MIC (2 μg/ml ) were selected for PD analysis. The ex vivo MIC in plasma of SH0165 was 0.25 μg/ml which was 8 times lower than that in TSB. The bacteriostatic, bactericidal and elimination activity (AUC24h /MIC) in serum were 26.35, 52.27 and 73.29 h based on the inhibitory sigmoid Emax modeling...
January 5, 2018: Oncotarget
Ke Dai, Xintian Wen, Yung-Fu Chang, Sanjie Cao, Qin Zhao, Xiaobo Huang, Rui Wu, Yong Huang, Qigui Yan, Xinfeng Han, Xiaoping Ma, Yiping Wen
Haemophilus parasuis is a member of the family Pasteurellaceae and a major causative agent of Glässer's disease. This bacterium is normally a benign swine commensal but may become a deadly pathogen upon penetration into multiple tissues, contributing to severe lesions in swine. We have established a successive natural transformation-based markerless mutation system in this species. However, the two-step mutation system requires screening of natural competent cells, and cannot delete genes which regulate natural competence per se...
2018: PeerJ
Shulin Fu, Huashan Liu, Lei Xu, Yinsheng Qiu, Yu Liu, Zhongyuan Wu, Chun Ye, Yongqing Hou, Chien-An Andy Hu
Haemophilus parasuis (H. parasuis) can cause vascular inflammatory injury, but the molecular basis of this effect remains unclear. In this study,we investigated the effect of the anti-inflammatory, anti-microbial and anti-oxidant agent, baicalin, on the nuclear factor (NF)-κB and NLRP3 inflammasome signaling pathway in pig primary aortic vascular endothelial cells. Activation of the NF-κB and NLRP3 inflammasome signaling pathway was induced in H. parasuis-infected cells. However, baicalin reduced the production of reactive oxygen species, apoptosis, and activation of the NF-κB and NLRP3 inflammasome signaling pathway in infected cells...
January 16, 2018: Scientific Reports
Annabelle Mathieu-Denoncourt, Corinne Letendre, Jean-Philippe Auger, Mariela Segura, Virginia Aragon, Sonia Lacouture, Marcelo Gottschalk
Streptococcus suis and Haemophilus parasuis are normal inhabitants of the porcine upper respiratory tract but are also among the most frequent causes of disease in weaned piglets worldwide, causing inflammatory diseases such as septicemia, meningitis and pneumonia. Using an in vitro model of infection with tracheal epithelial cells or primary alveolar macrophages (PAMs), it was possible to determine the interaction between S. suis serotype 2 and H. parasuis strains with different level of virulence. Within H...
January 6, 2018: Pathogens
Yongping Wen, Yiping Wen, Xintian Wen, Sanjie Cao, Xiaobo Huang, Rui Wu, Qin Zhao, Mafeng Liu, Yong Huang, Qigui Yan, Xinfeng Han, Xiaoping Ma, Ke Dai, Lingqiang Ding, Sitong Liu, Jian Yang
Haemophilus parasuis is an opportunistic pathogen and the causative agent of Glässer's disease in swine. This disease has high morbidity and mortality rates in swine populations, and is responsible for major economic losses worldwide. Survival of H. parasuis within the host requires mechanisms for coping with oxidative stress conditions. In many bacteria, OxyR is known to mediate protection against oxidative stress; however, little is known about the role of OxyR in H. parasuis. In the current study, an oxyR mutant strain was constructed in H...
February 15, 2018: Gene
Małgorzata Pomorska-Mól, Arkadiusz Dors, Krzysztof Kwit, Ewelina Czyżewska-Dors, Zygmunt Pejsak
BACKGROUND: Respiratory co-infections are important factor affecting the profitability of pigs production. Swine influenza virus (SIV) may predispose to secondary infection. Haemophilus parasuis (Hps) can be a primary pathogen or be associated with other pathogens such as SIV. To date, little is known about the effect of coinfection with SIV and Hps on the disease severity and inflammatory response and the role of Hps in the induction of pneumonia in the absence of other respiratory pathogens...
December 4, 2017: BMC Veterinary Research
Shuqing Liu, Wentao Li, Yang Wang, Changqin Gu, Xiaoli Liu, Catherine Charreyre, Shenxian Fan, Qigai He
BACKGROUND: Postweaning multisystemic wasting syndrome (PMWS) is an emerging disease in swine. Pigs with PMWS are often infected with a variety of other pathogens, including bacteria, viruses and mycoplasm, in addition to porcine circovirus type 2 (PCV2). PCV2 and Haemophilus parasuis serovar 4 (HPS4) coinfection remain epidemic in China. METHODS: Here we report construction of a three-week-old naturally farrowed, colostrum-deprived (NFCD) piglet's infection model and demonstrate that PCV2-infected piglets with the HPS4 coinfection increased the virulence of PCV2 and these pathogens interact acquired PMWS...
November 21, 2017: Virology Journal
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