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https://www.readbyqxmd.com/read/29346603/homologous-stress-adaptation-antibiotic-resistance-and-biofilm-forming-ability-of-salmonella-enterica-serovar-heidelberg-atcc8326-on-different-food-contact-surfaces-following-exposure-to-sublethal-chlorine-concentrations
#1
Tomi Obe, Rama Nannapaneni, Chander Shekhar Sharma, Aaron Kiess
Salmonella enterica serovar Heidelberg (American Type Culture Collection; ATCC 8326) was examined for the ability to adapt to the homologous stress of chlorine through exposure to increasing chlorine concentrations (25 ppm daily increments) in tryptic soy broth (TSB). The tested strain exhibited an acquired tolerance to chlorine in TSB with the tolerant cells growing in concentrations up to 400 ppm. In addition, the chlorine stressed cells displayed rugose morphology on tryptic soy agar (TSA) plates at 37°C...
January 13, 2018: Poultry Science
https://www.readbyqxmd.com/read/29346584/in-vitro-interactions-between-iap-antagonist-at406-and-azoles-against-planktonic-cells-and-biofilms-of-pathogenic-fungi-candida-albicans-and-exophiala-dermatitidis
#2
Yi Sun, Lujuan Gao, Chengyan He, Ming Li, Tongxiang Zeng
In vitro interactions of AT406, a novel IAP antagonist, and azoles including itraconazole, voriconazole, and fluconazole against planktonic cells and biofilms of Candida albicans and Exophiala dermatitidis were assessed via broth microdilution checkerboard technique. AT406 alone exhibited limited antifungal activity. However, synergistic effect between AT406 and fluconazole was observed against both planktonic cells and biofilms of C. albicans, including one fluconazole-resistant strain. Moreover, synergism was also demonstrated between AT406 and itraconazole against both planktonic cells and biofilms of E...
January 13, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29346565/apigenin-induces-cell-shrinkage-in-candida-albicans-by-membrane-perturbation
#3
Heejeong Lee, Eun-Rhan Woo, Dong Gun Lee
Apigenin, a natural flavone, has been well characterized for its their anticarcinogenic property; however, its bioactivity against pathogenic fungi has not been investigated in detail. In the present study, we examined the antifungal activity and mode of action of apigenin. Apigenin inhibited the growth of fungal pathogens, which induced superficial infection and reduced biofilm mass. Three-dimensional flow cytometric analysis demonstrated that apigenin induced morphological changes, especially cell shrinkage, in Candida albicans...
January 16, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29346561/floricolin-c-elicits-intracellular-ros-accumulation-and-disrupts-mitochondria-to-exert-fungicidal-action
#4
Ming Zhang, Wenqiang Chang, Hongzhuo Shi, Ying Li, Sha Zheng, Wei Li, Hongxiang Lou
Candida albicans, one most prevalent fungal pathogen, causes severe mucosal and invasive infections in predisposed individuals. The rise of fungal infection and drug-resistance demands the development of novel antifungal agents. In this study, we observed that floricolin C (FC), a p-terphenyl pigment from an endolichenic fungus, killed C. albicans cells at planktonic state or within biofilms through reactive oxygen species (ROS) accumulation. Further test revealed that FC could directly damage the mitochondria to cause ROS accumulation...
January 15, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29346398/dual-function-of-edta-with-silver-nanoparticles-for-root-canal-treatment-a-novel-modification
#5
Juan M Martinez-Andrade, Miguel Avalos-Borja, Alfredo R Vilchis-Nestor, Luis O Sanchez-Vargas, Ernestina Castro-Longoria
The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively...
2018: PloS One
https://www.readbyqxmd.com/read/29346379/a-putative-lateral-flagella-of-the-cystic-fibrosis-pathogen-burkholderia-dolosa-regulates-swimming-motility-and-host-cytokine-production
#6
Damien Roux, Matthew Schaefers, Bradley S Clark, Molly Weatherholt, Diane Renaud, David Scott, John J LiPuma, Gregory Priebe, Craig Gerard, Deborah R Yoder-Himes
Burkholderia dolosa caused an outbreak in the cystic fibrosis clinic at Boston Children's Hospital and was associated with high mortality in these patients. This species is part of a larger complex of opportunistic pathogens known as the Burkholderia cepacia complex (Bcc). Compared to other species in the Bcc, B. dolosa is highly transmissible; thus understanding its virulence mechanisms is important for preventing future outbreaks. The genome of one of the outbreak strains, AU0158, revealed a homolog of the lafA gene encoding a putative lateral flagellin, which, in other non-Bcc species, is used for movement on solid surfaces, attachment to host cells, or movement inside host cells...
2018: PloS One
https://www.readbyqxmd.com/read/29346268/effect-of-novel-quercetin-titanium-dioxide-decorated-multi-walled-carbon-nanotubes-nanocomposite-on-bacillus-subtilis-biofilm-development
#7
Diana S Raie, Eisha Mhatre, Doaa S El-Desouki, Ahmed Labena, Gamal El-Ghannam, Laila A Farahat, Tareq Youssef, Wolfgang Fritzsche, Ákos T Kovács
The present work was targeted to design a surface against cell seeding and adhering of bacteria, Bacillus subtilis. A multi-walled carbon nanotube/titanium dioxide nano-power was produced via simple mixing of carbon nanotube and titanium dioxide nanoparticles during the sol-gel process followed by heat treatment. Successfully, quercetin was immobilized on the nanocomposite via physical adsorption to form a quercetin/multi-walled carbon nanotube/titanium dioxide nanocomposite. The adhesion of bacteria on the coated-slides was verified after 24 h using confocal laser-scanning microscopy...
January 18, 2018: Materials
https://www.readbyqxmd.com/read/29345453/poly-glycidol-coating-on-ultrahigh-molecular-weight-polyethylene-for-reduced-biofilm-growth
#8
Jacob N Lockhart, Thomas J Spoonmore, Michael W McCurdy, Bridget R Rogers, Scott A Guelcher, Eva Harth
Semibranched poly(glycidol) (PG-OH) and poly(glycidol allylglycidyl ether) (PG-Allyl) coatings were formed on ultrahigh molecular weight polyethylene (UMWPE) in a unique two-step process which included radiation of UHMWPE followed by grafting of PG-OH or PG-Allyl to the surface via free radical cross-linking. Resulting surfaces were extensively characterized by FTIR-ATR, XPS, fluorescent microscopy, and contact goniometry. The performance was evaluated using the most prominent biofilm-forming bacteria Staphylococcus aureus for 24 and 48 h...
January 18, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29345080/environmental-adaptation-and-vertical-dissemination-of-esbl-pampc-producing-escherichia-coli-in-an-integrated-broiler-production-chain-in-the-absence-of-an-antibiotic-treatment
#9
Michaela Projahn, Katrin Daehre, Torsten Semmler, Sebastian Guenther, Uwe Roesler, Anika Friese
High prevalence numbers of extended-spectrum beta-lactamase- (ESBL-)/plasmid-mediated AmpC beta-lactamase- (pAmpC-) producing Escherichia coli in broiler chicken and their distribution along the broiler production chain is an ongoing problem in food production. We, therefore, investigated resistant isolates along the broiler production chain to determine whether there is a constantly occurring direct vertical transmission of the ESBL-/pAmpC-producing E. coli from the parent flocks to their offspring or not...
January 17, 2018: Microbial Biotechnology
https://www.readbyqxmd.com/read/29344966/quorum-sensing-molecule-dihydroxy-2-3-pentanedione-and-its-analogs-as-regulators-of-epithelial-integrity
#10
S Elmanfi, X Ma, H O Sintim, E Könönen, S Syrjänen, U K Gursoy
BACKGROUND AND OBJECTIVE: Quorum-sensing molecules regulate the behavior of bacteria within biofilms and at the same time elicit an immune response in host tissues. Our aim was to investigate the regulatory role of dihydroxy-2,3-pentanedione (DPD), the precursor of universal autoinducer-2 (AI-2), and its analogs (ethyl-DPD, butyl-DPD and isobutyl-DPD) in the integrity of gingival epithelial cells. MATERIAL AND METHODS: Human gingival keratinocytes were incubated with four concentrations (10 μmol L-1 , 1 μmol L-1 , 100 nmol L-1 and 10 nmol L-1 ) of DPD and its analogs for 24 hours...
January 17, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29344694/towards-a-better-production-of-bacterial-exopolysaccharides-by-controlling-genetic-as-well-as-physico-chemical-parameters
#11
REVIEW
Dipanjan Sengupta, Sriparna Datta, Dipa Biswas
Bacterial extracellular polymeric substances, which are basically bacterial metabolites, have currently become a subject of great concern of modern day microbiologists and biotechnologists. Among these metabolites, bacterial exopolysaccharides or EPS, in particular, have gained a significant importance. EPS are formed by the bacteria in their late exponential or stationary phase of growth under special situations for specific purposes. They take part in the formation of bacterial biofilms. There is a great diversity in the types of EPS...
January 17, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29343818/a-surfactant-polymer-dressing-potentiates-antimicrobial-efficacy-in-biofilm-disruption
#12
Piya Das Ghatak, Shomita S Mathew-Steiner, Priyanka Pandey, Sashwati Roy, Chandan K Sen
A 100% water-soluble surfactant polymer dressing (SPD) that is bio-compatible and non-ionic has been reported to improve wound closure in preliminary clinical studies. The mechanism of action of SPD in wound healing remains unclear. Biofilm infection is a significant problem that hinders proper wound closure. The objective of this study was to characterize the mechanism of action of SPD inhibition of bacterial biofilm development. Static biofilms (48 h) of the primary wound pathogens Pseudomonas aeruginosa (PA01), Staphylococcus aureus (USA300) were grown on polycarbonate membranes and treated with SPD with and without antibiotics for an additional 24 h...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343757/the-early-conversion-of-deep-sea-wood-falls-into-chemosynthetic-hotspots-revealed-by-in-situ-monitoring
#13
D Kalenitchenko, E Péru, L Contreira Pereira, C Petetin, P E Galand, N Le Bris
Wood debris on the ocean floor harbor flourishing communities, which include invertebrate taxa thriving in sulfide-rich habitats belonging to hydrothermal vent and methane seep deep-sea lineages. The formation of sulfidic niches from digested wood material produced by woodborers has been known for a long time, but the temporal dynamics and sulfide ranges encountered on wood falls remains unknown. Here, we show that wood falls are converted into sulfidic hotpots, before the colonization by xylophagaid bivalves...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343729/drug-self-assembly-for-synthesis-of-highly-loaded-antimicrobial-drug-silica-particles
#14
Cameron A Stewart, Yoav Finer, Benjamin D Hatton
Antimicrobial drug release from biomaterials for orthopedic repair and dental restorations can prevent biofilm growth and caries formation. Carriers for drug incorporation would benefit from long-term drug storage, controlled release, and structural stability. Mesoporous silica, synthesized through a co-assembly of silica and surfactant template, is an ideal drug encapsulation scaffold that maintains structural integrity upon release. However, conventional loading of drug within meso-silica pores via concentration-gradient diffusion limits the overall payload, concentration uniformity, and drug release control...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343164/inflammatory-and-oxidative-responses-in-pregnancies-with-obesity-and-periodontal-disease
#15
Marta Zambon, Chiara Mandò, Alessandra Lissoni, Gaia Maria Anelli, Chiara Novielli, Manuela Cardellicchio, Roberto Leone, Marta Noemi Monari, Maddalena Massari, Irene Cetin, Silvio Abati
BACKGROUND: Maternal obesity is related to immunologic and inflammatory systemic modifications that may worsen the pregnancy inflammatory status. Hormonal changes during pregnancy can adversely affect oral biofilms and oral health initiating or worsening periodontal diseases, with enhanced local and systemic oxidative stress and inflammation. OBJECTIVE: The aim of this study was to examine the relationship between local salivary and systemic parameters of oxidative stress and inflammation in relation to obesity and periodontal diseases...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29342911/effects-of-acanthopanax-senticosus-on-brain-injury-induced-by-simulated-spatial-radiation-in-mouse-model-based-on-pharmacokinetics-and-comparative-proteomics
#16
Yingyu Zhou, Cuilin Cheng, Denis Baranenko, Jiaping Wang, Yongzhi Li, Weihong Lu
The active compounds in Acanthopanax senticosus (AS) have different pharmacokinetic characteristics in mouse models. Cmax and AUC of Acanthopanax senticosus polysaccharides (ASPS) were significantly reduced in radiation-injured mice, suggesting that the blood flow of mouse was blocked or slowed, due to the pathological state of ischemia and hypoxia, which are caused by radiation. In contrast, the ability of various metabolizing enzymes to inactivate, capacity of biofilm transport decrease, and lessening of renal blood flow accounts for radiation, resulting in the accumulation of syringin and eleutheroside E in the irradiated mouse...
January 15, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29342354/a-highly-dynamic-loop-of-the-pseudomonas-aeruginosa-pa14-type-iv-pilin-is-essential-for-pilus-assembly
#17
Ylan Nguyen, Stephen Boulton, E Tyler McNicholl, Madoka Akimoto, Hanjeong Harvey, Francisca Aidoo, Giuseppe Melacini, Lori L Burrows
Type IVa pili (T4aP) are long, thin surface filaments involved in attachment, motility, biofilm formation, and DNA uptake. They are important virulence factors for many bacteria, including Pseudomonas aeruginosa, an opportunistic pathogen and common cause of hospital-acquired infections. Each helical filament contains thousands of monomers of the major pilin subunit, PilA. Each P. aeruginosa strain expresses one of five phylogenetically distinct major pilins, which vary in sequence and the nature of their associated accessory protein(s)...
January 17, 2018: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29342260/interaction-between-staphylococcus-aureus-and-pseudomonas-aeruginosa-is-beneficial-for-colonisation-and-pathogenicity-in-a-mixed-biofilm
#18
Patrícia M Alves, Eide Al-Badi, Cathryn Withycombe, Paul M Jones, Kevin J Purdy, Sarah E Maddocks
Debate regarding the co-existence of Staphylococcus aureus and Pseudomonas aeruginosa in wounds remains contentious, with the dominant hypothesis describing a situation akin to niche partitioning, whereby both microorganisms are present but occupy distinct regions of the wound without interacting. In contrast, we hypothesised that these microorganisms do interact during early co-colonisation in a manner beneficial to both bacteria. We assessed competitive interaction between S. aureus and P. aeruginosa in biofilm cultured for 24-72 h and bacterial aggregates analogous to those observed in early ( < 24h) biofilm formation, and interaction with human keratinocytes...
January 12, 2018: Pathogens and Disease
https://www.readbyqxmd.com/read/29342189/the-microbiota-metabolite-indole-inhibits-salmonella-virulence-involvement-of-the-phopq-two-component-system
#19
Nandita Kohli, Zeni Crisp, Rebekah Riordan, Michael Li, Robert C Alaniz, Arul Jayaraman
The microbial community present in the gastrointestinal tract is an important component of the host defense against pathogen infections. We previously demonstrated that indole, a microbial metabolite of tryptophan, reduces enterohemorrhagic Escherichia coli O157:H7 attachment to intestinal epithelial cells and biofilm formation, suggesting that indole may be an effector/attenuator of colonization for a number of enteric pathogens. Here, we report that indole attenuates Salmonella Typhimurium (Salmonella) virulence and invasion as well as increases resistance to colonization in host cells...
2018: PloS One
https://www.readbyqxmd.com/read/29342119/novel-hybrid-formulations-based-on-thiourea-derivatives-and-core-shell-fe%C3%A2-o%C3%A2-c18-nanostructures-for-the-development-of-antifungal-strategies
#20
Carmen Limban, Alexandru Vasile Missir, Miron Teodor Caproiu, Alexandru Mihai Grumezescu, Mariana Carmen Chifiriuc, Coralia Bleotu, Luminita Marutescu, Marius Toma Papacocea, Diana Camelia Nuta
The continuously increasing global impact of fungal infections is requiring the rapid development of novel antifungal agents. Due to their multiple pharmacological activities, thiourea derivatives represent privileged candidates for shaping new drugs. We report here the preparation, physico-chemical characterization and bioevaluation of hybrid nanosystems based on new 2-((4-chlorophenoxy)methyl)-N-(substituted phenylcarbamo-thioyl)benzamides and Fe₃O₄@C18 core@shell nanoparticles. The new benzamides were prepared by an efficient method, then their structure was confirmed by spectral studies and elemental analysis and they were further loaded on Fe₃O₄@C18 nanostructures...
January 17, 2018: Nanomaterials
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