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https://www.readbyqxmd.com/read/28432695/recent-strategies-towards-microfluidic-based-surface-enhanced-raman-spectroscopy
#1
REVIEW
Anna Tycova, Jan Prikryl, Frantisek Foret
Surface enhanced Raman spectroscopy (SERS) is an extremely powerful analytical tool, which not only yields information about the molecular structure of the analyte in the form of characteristic vibrational spectrum but also gives sensitivities approaching those in fluorescence spectroscopy. The SERS measurement on the microfluidic platform provides possibility to manufacture the device with design perfectly fulfilling the needs of the application with minimal sample consumption. This review aims at describing basic strategies for SERS measurement in microfluidic devices published in the last decade and covers current trends in microfluidics with SERS detection in the field of bioanalysis and approaches towards on-line coupling of liquid-based separation techniques with SERS detection...
April 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28432532/a-portable-battery-powered-microfluidic-impedance-cytometer-with-smartphone-readout-towards-personal-health-monitoring
#2
Niloy Talukder, Abbas Furniturewalla, Tuan Le, Matthew Chan, Shreyas Hirday, Xinnan Cao, Pengfei Xie, Zhongtian Lin, Azam Gholizadeh, Steve Orbine, Mehdi Javanmard
We present a portable system for personalized blood cell counting consisting of a microfluidic impedance cytometer and portable analog readout electronics, feeding into an analog-to-digital converter (ADC), and being transmitted via Bluetooth to a user-accessible mobile application. We fabricated a microfluidic impedance cytometer with a novel portable analog readout. The novel design of the analog readout, which consists of a lock-in-amplifier followed by a high-pass filter stage for subtraction of drift and DC offset, and a post-subtraction high gain stage, enables detection of particles and cells as small as 1 μm in diameter, despite using a low-end 8-bit ADC...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28432531/effectiveness-of-nanoencapsulated-methotrexate-against-osteosarcoma-cells-in-vitro-cytotoxicity-under-dynamic-conditions
#3
O Mitxelena-Iribarren, C L Hisey, M Errazquin-Irigoyen, Y González-Fernández, E Imbuluzqueta, M Mujika, M J Blanco-Prieto, S Arana
Cancer is a leading cause of mortality in the world, with osteosarcoma being one of the most common types among children between 1 and 14 years old. Current treatments including preoperative chemotherapy, surgery and postoperative chemotherapy produce several side effects with limited effectiveness. The use of lipid nanoparticles as biodegradable shells for controlled drug delivery shows promise as a more effective and targeted tumor treatment. However, in vitro validation of these vehicles is limited due to fluid stagnation in current techniques, in which nanoparticles sediment onto the bottom of the wells killing the cells by asphyxiation...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28432530/an-integrated-microsystem-with-dielectrophoresis-enrichment-and-impedance-detection-for-detection-of-escherichia-coli
#4
Renjie Wang, Yi Xu, Haitao Liu, Jinlan Peng, Joseph Irudayaraj, Feiyun Cui
An integrated microsystem device with matched interdigitated microelectrode chip was fabricated for enrichment and detection of Escherichia coli O157:H7. The microsystem has integrated with positive dielectrophoresis (pDEP) enrichment and in situ impedance detection, whose total volume is only 3.0 × 10(-3) m(3), and could provide impedance testing voltages of 0 ~ 10 V, detection frequencies of 1 KHz ~ 1 MHz, DEP excitation signals with amplitude of 0 ~ 10 Vpp and frequencies of 1KHz ~ 1 MHz, which fully meets the demands of pDEP enrichment and impedance detection for bacteria...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28432341/detection-of-red-blood-cell-surface-antigens-by-probe-triggered-cell-collision-and-flow-retardation-in-an-autonomous-microfluidic-system
#5
Éva Sautner, Krisztián Papp, Eszter Holczer, Eszter L Tóth, Rita Ungai-Salánki, Bálint Szabó, Péter Fürjes, József Prechl
Microfluidic devices exploit combined physical, chemical and biological phenomena that could be unique in the sub-millimeter dimensions. The current goal of development of Point-of-Care (POC) medical devices is to extract the biomedical information from the blood. We examined the characteristics of blood flow in autonomous microfluidic devices with the aim to realize sensitive detection of interactions between particulate elements of the blood and the appropriately modified surfaces of the system. As a model experiment we demonstrated the fast analysis of the AB0 blood group system...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28431925/analysis-of-pm2-5-induced-cytotoxicity-in-human-hacat-cells-based-on-a-microfluidic-system
#6
Yuxiao Zhang, Lulu Zheng, Jiang Tuo, Qi Liu, Xinlian Zhang, Zhixuan Xu, Sixiu Liu, Guodong Sui
Human exposure to PM2.5 causes several adverse health effects. Skin is the first barrier against harmful environmental substances and can has direct contact with PM2.5, but there is no study about PM2.5-induced cytotoxicity in human skin cells on the molecular level partially due to the shortcomings of traditional research methods. In present study, we established a microfluidic system including a cell culture chip integrated with a high-throughput protein microarray chip to investigate the mechanism of PM2...
April 18, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28430559/heart-on-a-chip-an-investigation-of-the-influence-of-static-and-perfusion-conditions-on-cardiac-h9c2-cell-proliferation-morphology-and-alignment
#7
Anna Kobuszewska, Ewelina Tomecka, Kamil Zukowski, Elzbieta Jastrzebska, Michal Chudy, Artur Dybko, Philippe Renaud, Zbigniew Brzozka
Lab-on-a-chip systems are increasingly used as tools for cultures and investigation of cardiac cells. In this article, we present how the geometry of microsystems and microenvironmental conditions (static and perfusion) influence the proliferation, morphology, and alignment of cardiac cells (rat cardiomyoblasts-H9C2). Additionally, studies of cell growth after incubation with verapamil hydrochloride were performed. For this purpose, poly(dimethylsiloxane) (PDMS)/glass microfluidic systems with three different geometries of microchambers (a circular chamber, a longitudinal channel, and three parallel microchannels separated by two rows of micropillars) were prepared...
April 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28430279/microcontact-printing-with-aminosilanes-creating-biomolecule-micro-and-nanoarrays-for-multiplexed-microfluidic-bioassays
#8
Shivani Sathish, Sébastien G Ricoult, Kazumi Toda-Peters, Amy Q Shen
Microfluidic systems integrated with protein and DNA micro- and nanoarrays have been the most sought-after technologies to satisfy the growing demand for high-throughput disease diagnostics. As the sensitivity of these systems relies on the bio-functionalities of the patterned recognition biomolecules, the primary concern has been to develop simple technologies that enable biomolecule immobilization within microfluidic devices whilst preserving bio-functionalities. To address this concern, we introduce a two-step patterning approach to create micro- and nanoarrays of biomolecules within microfluidic devices...
April 21, 2017: Analyst
https://www.readbyqxmd.com/read/28429415/rod-shaped-neural-units-for-aligned-3d-neural-network-connection
#9
Midori Kato-Negishi, Hiroaki Onoe, Akane Ito, Shoji Takeuchi
This paper proposes neural tissue units with aligned nerve fibers (called rod-shaped neural units) that connect neural networks with aligned neurons. To make the proposed units, 3D fiber-shaped neural tissues covered with a calcium alginate hydrogel layer are prepared with a microfluidic system and are cut in an accurate and reproducible manner. These units have aligned nerve fibers inside the hydrogel layer and connectable points on both ends. By connecting the units with a poly(dimethylsiloxane) guide, 3D neural tissues can be constructed and maintained for more than two weeks of culture...
April 21, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28426762/human-microrna-299-3p-decreases-invasive-behavior-of-cancer-cells-by-downregulation-of-oct4-expression-and-causes-apoptosis
#10
Axel R Göhring, Stefanie Reuter, Joachim H Clement, Xinlai Cheng, Jannick Theobald, Stefan Wölfl, Ralf Mrowka
PURPOSE: Oct4 was reported to be one of the most important pluripotency transcription factors in the biology of stem cells including cancer stem cells, and progressed malignant cells. Here we report the investigation of gene expression control of Oct4 by selected human microRNAs and the physiological effect of Oct4 silencing in invasive cancer cells. METHODS AND RESULTS: High throughput luciferase activity assay revealed the microRNA-299-3p to be the most effective in reducing gene expression of Oct4, which was confirmed by Western blot analysis and Oct4 promoter activity in a target luciferase assay...
2017: PloS One
https://www.readbyqxmd.com/read/28426707/rare-cell-isolation-and-recovery-on-open-channel-microfluidic-chip
#11
Taisuke Masuda, Woneui Song, Hayao Nakanishi, Wu Lei, Anas Mohd Noor, Fumihito Arai
The ability to accurately detect and analyze rare cells in a cell population is critical not only for the study of disease progression but also for next flow cytometry systems in clinical application. Here, we report the development of a prototype device, the 'Rare cell sorter', for isolating and recovering single rare cells from whole blood samples. On this device, we utilized an open-channel microfluidic chip for rare cell isolation. And the advantage of open-channel allows us to recover the isolated rare cell directly from the chip...
2017: PloS One
https://www.readbyqxmd.com/read/28426555/creating-artificial-lymphoid-tissues-to-study-immunity-and-hematological-malignancies
#12
Shivem B Shah, Ankur Singh
PURPOSE OF REVIEW: The specialized microenvironments of lymphoid tissue affect immune cell function and progression of disease. However, current animal models are low throughput and a large number of human diseases are difficult to model in animals. Animal models are less amenable to manipulation of tissue niche components, signalling pathways, epigenetics, and genome editing than ex vivo models. On the other hand, conventional 2D cultures lack the physiological relevance to study precise microenvironmental interactions...
April 19, 2017: Current Opinion in Hematology
https://www.readbyqxmd.com/read/28426159/open-microfluidic-gel-electrophoresis-rapid-and-low-cost-separation-and-analysis-of-dna-at-the-nanoliter-scale
#13
Ludwig Gutzweiler, Tobias Gleichmann, Laurent Tanguy, Peter Koltay, Roland Zengerle, Lutz Riegger
Gel electrophoresis is one of the most applied and standardized tools for separation and analysis of macromolecules and their fragments in academic research and in industry. In this work we present a novel approach for conducting on-demand electrophoretic separations of DNA molecules in open microfluidic (OM) systems on planar polymer substrates. The approach combines advantages of slab gel, capillary- & chip-based methods offering low consumable costs (<0.1 $) circumventing cost intensive microfluidic chip fabrication, short process times (5 minutes per analysis) and high sensitivity (4 ng/μl dsDNA) combined with reasonable resolution (17 bases)...
April 20, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28426080/c-reactive-protein-and-interleukin-6-microfluidic-immunoassays-with-on-chip-pre-stored-reagents-and-centrifugo-pneumatic-liquid-control
#14
Y Zhao, G Czilwik, V Klein, K Mitsakakis, R Zengerle, N Paust
We present a fully automated centrifugal microfluidic method for particle based protein immunoassays. Stick-pack technology is employed for pre-storage and release of liquid reagents. Quantitative layout of centrifugo-pneumatic particle handling, including timed valving, switching and pumping is assisted by network simulations. The automation is exclusively controlled by the spinning frequency and does not require any additional means. New centrifugal microfluidic process chains are developed in order to sequentially supply wash buffer based on frequency dependent stick-pack opening and pneumatic pumping to perform two washing steps from one stored wash buffer; pre-store and re-suspend functionalized microparticles on a disk; and switch between the path of the waste fluid and the path of the substrate reaction product with 100% efficiency...
April 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28426055/droplet-manipulation-on-a-structured-shape-memory-polymer-surface
#15
Jun Kyu Park, Seok Kim
While methods for dynamic tuning of surface wettability to manipulate water droplets have been widely explored for many applications including digital microfluidics, those based on dynamically changeable surface morphology have remained challenging to achieve. In this work, we present a structured shape memory polymer (SMP) surface which shows dynamically tunable surface wettability through changeable surface morphology in order to manipulate water droplets. The structured SMP surface involves a SMP pillar array consisting of nanotextured small and large pillars which can change its morphology between permanent and temporary shapes upon thermomechanical loading...
April 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28425667/soft-multifunctional-composites-and-emulsions-with-liquid-metals
#16
REVIEW
Navid Kazem, Tess Hellebrekers, Carmel Majidi
Binary mixtures of liquid metal (LM) or low-melting-point alloy (LMPA) in an elastomeric or fluidic carrier medium can exhibit unique combinations of electrical, thermal, and mechanical properties. This emerging class of soft multifunctional composites have potential applications in wearable computing, bio-inspired robotics, and shape-programmable architectures. The dispersion phase can range from dilute droplets to connected networks that support electrical conductivity. In contrast to deterministically patterned LM microfluidics, LMPA- and LM-embedded elastomer (LMEE) composites are statistically homogenous and exhibit effective bulk properties...
April 20, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28425543/in-situ-nuclear-magnetic-resonance-microimaging-of-live-biofilms-in-a-microchannel
#17
R S Renslow, M J Marshall, A E Tucker, W B Chrisler, X-Y Yu
Biofilms are comprised of microbial cells and an extracellular polymeric substance (EPS) matrix that supports interactions between community members and with the local environment. The highly hydrated EPS matrix makes the application of many biofilm visualization techniques difficult. Hence, to better visualize how biofilms interact with their environment, there is a need for imaging techniques to monitor hydrated state biofilm dynamics. We employed an in situ dynamic approach to construct label-free images of biofilms...
April 20, 2017: Analyst
https://www.readbyqxmd.com/read/28425472/label-free-single-cell-separation-and-imaging-of-cancer-cells-using-an-integrated-microfluidic-system
#18
Maria Antfolk, Soo Hyeon Kim, Saori Koizumi, Teruo Fujii, Thomas Laurell
The incidence of cancer is increasing worldwide and metastatic disease, through the spread of circulating tumor cells (CTCs), is responsible for the majority of the cancer deaths. Accurate monitoring of CTC levels in blood provides clinical information supporting therapeutic decision making, and improved methods for CTC enumeration are asked for. Microfluidics has been extensively used for this purpose but most methods require several post-separation processing steps including concentration of the sample before analysis...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28424414/integrated-analysis-of-promoter-methylation-and-expression-of-telomere-related-genes-in-breast-cancer
#19
Jianfu Heng, Fan Zhang, Xinwu Guo, Lili Tang, Limin Peng, Xipeng Luo, Xunxun Xu, Shouman Wang, Lizhong Dai, Jun Wang
Telomeres at the ends of eukaryotic chromosomes play a critical role in tumorgenesis. Using microfluidic PCR and next-generation bisulfite sequencing technology, we investigated the promoter methylation of 29 telomere related genes in paired tumor and normal tissues from 184 breast cancer patients. The expression of significantly differentially methylated genes was quantified using qPCR method.We observed that the average methylation level of the 29 telomere related genes was significant higher in tumor than that in normal tissues (P = 4...
March 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423001/pre-hatching-embryo-dependent-and-independent-programming-of-endometrial-function-in-cattle
#20
Mariana Sponchiado, Nathália Souza Gomes, Patrícia Kubo Fontes, Thiago Martins, Maite Del Collado, Athos de Assumpção Pastore, Guilherme Pugliesi, Marcelo Fábio Gouveia Nogueira, Mario Binelli
The bovine pre-implantation embryo secretes bioactive molecules from early development stages, but effects on endometrial function are reported to start only after elongation. Here, we interrogated spatially defined regions of the endometrium transcriptome for responses to a day 7 embryo in vivo. We hypothesize that exposure to an embryo changes the abundance of specific transcripts in the cranial region of the pregnant uterine horn. Endometrium was collected from the uterotubal junction (UTJ), anterior (IA), medial (IM) and posterior (IP) regions of the uterine horn ipsilateral to the CL 7 days after estrus from sham-inseminated (Con) or artificially inseminated, confirmed pregnant (Preg) cows...
2017: PloS One
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