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https://www.readbyqxmd.com/read/27920196/regulation-of-turkey-myogenic-satellite-cell-migration-by-micrornas-mir-128-and-mir-24
#1
S G Velleman, R L Harding
Myogenic satellite cells are an adult stem cell responsible for all post-hatch muscle growth in poultry. As a stem cell population, satellite cells are highly heterogeneous, but the origin of this heterogeneity remains unclear. Heterogeneity is, in part, regulated by gene expression. One method of endogenous gene regulation that may contribute to heterogeneity is microRNAs (miRNAs). Two miRNAs previously shown to regulate poultry myogenic satellite cell proliferation and differentiation, miR-128 and miR-24, were studied to determine if they also affected satellite cell migration...
December 5, 2016: Poultry Science
https://www.readbyqxmd.com/read/27919618/electric-field-induced-suppression-of-pten-drives-epithelial-to-mesenchymal-transition-via-mtorc1-activation
#2
Tiantian Yan, Xupin Jiang, Xiaowei Guo, Wen Chen, Di Tang, Junhui Zhang, Xingyue Zhang, Dongxia Zhang, Qiong Zhang, Jiezhi Jia, Yuesheng Huang
BACKGROUND: Naturally occurring electric fields (EFs) are an intrinsic property of wounds. Endogenous EFs in skin wounds play critical roles in the dynamic and well-ordered biological process of wound healing. The epithelial-to-mesenchymal transition (EMT) allows keratinocytes to transition from sedentary cells to motile cells, facilitating wound healing. However, EMT-related studies have been performed without considering endogenous EFs. Thus, the relationship between electrical signals and the EMT remain elusive...
November 18, 2016: Journal of Dermatological Science
https://www.readbyqxmd.com/read/27918105/resveratrol-inhibits-proliferation-invasion-and-epithelial-mesenchymal-transition-by-increasing-mir-200c-expression-in-hct-116-colorectal-cancer-cells
#3
Fatemeh Karimi Dermani, Massoud Saidijam, Razieh Amini, Ali Mahdavinezhad, Korosh Heydari, Rezvan Najafi
OBJECTIVE: Colorectal cancer (CRC) is one of the most common malignancies, associated with high rates of relapse. A notable challenge in treatment is low response rate to current therapies for advanced CRC. The miR-200c plays an essential role in tumor suppression by inhibiting epithelial-mesenchymal transition (EMT). Resveratrol, a natural compound found in red wine, reveals anti-cancer properties in several types of cancers such as CRC. The aim of current study was to evaluate the effects of resveratrol on proliferation, apoptosis and invasion of HCT-116 cells and also expression of EMT-related genes in presences or absence of miR-200c...
December 5, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27917836/the-crystal-structure-of-dihydrodipicolinate-reductase-from-the-human-pathogenic-bacterium-bartonella-henselae-strain-houston-1-at-2-3%C3%A2-%C3%A3-resolution
#4
Ali R Cala, Maria T Nadeau, Jan Abendroth, Bart L Staker, Alexandra R Reers, Anthony W Weatherhead, Renwick C J Dobson, Peter J Myler, André O Hudson
In bacteria, the second committed step in the diaminopimelate/lysine anabolic pathways is catalyzed by the enzyme dihydrodipicolinate reductase (DapB). DapB catalyzes the reduction of dihydrodipicolinate to yield tetrahydrodipicolinate. Here, the cloning, expression, purification, crystallization and X-ray diffraction analysis of DapB from the human-pathogenic bacterium Bartonella henselae, the causative bacterium of cat-scratch disease, are reported. Protein crystals were grown in conditions consisting of 5%(w/v) PEG 4000, 200 mM sodium acetate, 100 mM sodium citrate tribasic pH 5...
December 1, 2016: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/27917311/rock-inhibition-promotes-attachment-proliferation-and-wound-closure-in-human-embryonic-stem-cell-derived-retinal-pigmented-epithelium
#5
Roxanne H Croze, William J Thi, Dennis O Clegg
PURPOSE: Nonexudative (dry) age-related macular degeneration (AMD), a leading cause of blindness in the elderly, is associated with the loss of retinal pigmented epithelium (RPE) cells and the development of geographic atrophy, which are areas devoid of RPE cells and photoreceptors. One possible treatment option would be to stimulate RPE attachment and proliferation to replace dying/dysfunctional RPE and bring about wound repair. Clinical trials are underway testing injections of RPE cells derived from pluripotent stem cells to determine their safety and efficacy in treating AMD...
November 2016: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/27917272/the-hedgehog-gli-1-signaling-pathways-is-involved-in-the-inhibitory-effect-of-resveratrol-on-human-colorectal-cancer-hct116-cells
#6
Zhou Du, Feng Zhou, Zengrong Jia, Beishi Zheng, Shaoliang Han, Jun Cheng, Guanbao Zhu, Ping Huang
OBJECTIVES: The study aimed to investigate the effects of resveratrol on colorectal cancer HCT116 cells, including cell viability, apoptosis, and migration, and the partial mechanisms focused on hedgehog/gli-1 signaling pathways. MATERIALS AND METHODS: We chose the appropriate time and concentration of recombinant human Sonic hedgehog (Shh) stimulation by cell viability. The proportion of cell apoptosis was detected by flow cytometry; HCT116 cell migration was measured by scratch test; the expression of Ptch, Smo, and Gli-1 was measured by Western blot analysis...
November 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27911920/sirna-targeting-mtor-effectively-prevents-the-proliferation-and-migration-of-human-lens-epithelial-cells
#7
Chunmei Zhang, Jingjing Liu, Na Jin, Guiming Zhang, Yahui Xi, Hongling Liu
Posterior capsule opacification (PCO) is the most common complication that causes visual decrease after extracapsular cataract surgery. The primary cause of PCO formation is the proliferation of the residual lens epithelial cells (LECs). The mammalian target of rapamycin (mTOR) plays an important role in the growth and migration of LECs. In the current study, we used small interfering RNA (siRNA) to specifically attenuate mTOR in human lens epithelial B3 cells (HLE B3). We aimed to examine the effect of mTOR-siRNA on the proliferation, migration and epithelial-to-mesenchymal transition (EMT) of HLE B3 cells and explore the underlying mechanisms...
2016: PloS One
https://www.readbyqxmd.com/read/27911377/hybrid-cut-an-improved-sectioning-method-for-recalcitrant-plant-tissue-samples
#8
Tien-Kuan Chen, Hui-Ting Yang, Su-Chiung Fang, Yi-Chen Lien, Ting-Ting Yang, Swee-Suak Ko
Maintaining plant section integrity is essential for studying detailed anatomical structures at the cellular, tissue, or even organ level. However, some plant cells have rigid cell walls, tough fibers and crystals (calcium oxalate, silica, etc.), and high water content that often disrupt tissue integrity during plant tissue sectioning. This study establishes a simple Hybrid-Cut tissue sectioning method. This protocol modifies a paraffin-based sectioning technique and improves the integrity of tissue sections from different plants...
November 23, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27906919/the-scratch-test-for-identifying-the-lower-liver-edge-is-at-least-as-accurate-as-percussion-and-is-significantly-more-effective-for-young-trainees-a-randomised-comparative-trial
#9
Jesse Fischer, Justin Hegarty, Anna Ashcroft, Christopher Ma Frampton, Murray L Barclay
BACKGROUND: Clinical examination of the liver requires experience to achieve accuracy. The scratch test is a simple technique to identify the lower liver edge and enhance liver palpation, and may be easier for trainees. AIM: We aimed to evaluate the accuracy of the scratch test compared to percussion at different levels of medical training. METHOD: Eight examiners, from trainee intern to consultant level, were randomised to scratch or percussion testing, followed by liver palpation, on 50 subjects...
December 2, 2016: New Zealand Medical Journal
https://www.readbyqxmd.com/read/27906614/development-of-new-forms-of-self-injurious-behavior-following-total-dental-extraction-in-lesch-nyhan-disease
#10
Livia Gisbert de la Cuadra, Rosa J Torres, Luis M Beltrán, Arantxa Sánchez, Juan G Puig
We report two Lesch-Nyhan Disease (LND) patients who developed new forms of self-injurious behavior following total dental extraction. Patients 1 and 2 were submitted to total teeth extraction at the age of 13 and 8 years, respectively, due to continuous self-biting, not prevented by mouth guards. Severity of dystonia was markedly reduced and quality of life improved. After 12 and 17 months, respectively, patient 1 started rubbing one foot against other and scratching toenails with his hands, and patient 2 stuck his legs and feet against hard objects...
December 2016: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/27906093/microrna-140-5p-inhibits-colorectal-cancer-invasion-and-metastasis-by-targeting-adamts5-and-igfbp5
#11
Lihui Yu, Ying Lu, Xiaocui Han, Wenyue Zhao, Jiazhi Li, Jun Mao, Bo Wang, Jie Shen, Shujun Fan, Lu Wang, Mei Wang, Lianhong Li, Jianwu Tang, Bo Song
BACKGROUND: Colorectal cancer (CRC) is one of the most common malignancies in the world. microRNA-140-5p (miR-140) has been shown to be involved in cartilage development and osteoarthritis (OA) pathogenesis. Some contradictions still exist concerning the role of miR-140 in tumor progression and metastasis, and the underlying mechanism is uncertain. METHODS: Immunohistochemistry was performed to determine the expressions of ADAMTS5 and IGFBP5 in CRC tissues. Human CRC cell lines HCT116 and RKO were transfected with miR-140 mimic, inhibitor, or small interfering RNA (siRNA) against ADAMTS5 or IGFBP5, respectively, using oligofectamine or lipofectamine 2000...
December 1, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27905196/from-scratch-developing-a-hepatic-resection-service-for-metastatic-colorectal-cancer
#12
Neil Wylie, Phillip Hider, Delwyn Armstrong, Kheman Rajkomar, Sanket Srinivasa, Michael Rodgers, Anna Brown, Jonathan Koea
BACKGROUND: Waitemata District Health Board has New Zealand's largest catchment and busiest colorectal unit. The upper gastrointestinal unit was established in 2005, in part to provide a hepatic resection service for patients with colorectal carcinoma metastatic to the liver. The aim of this investigation was to report on quality indicators for the hepatic resection of colorectal carcinoma in the development of a regional resection service. METHODS: Prospectively collected data on patients undergoing hepatic resection for colorectal carcinoma between 2005 and 2014 was reviewed and correlated with costing data and national hepatic resection rates...
December 1, 2016: ANZ Journal of Surgery
https://www.readbyqxmd.com/read/27904090/effect-of-bidirectional-loading-on-contact-and-force-characteristics-under-a-newly-developed-masticatory-simulator-with-a-dual-direction-loading-system
#13
Weerachai Singhatanadgit, Puttiporn Junkaew, Pairod Singhatanadgid
Mechanical responses of the test specimen under bidirectional and unidirectional loading were investigated using a newly developed masticatory simulator. The simulator adopted a four-bar linkage mechanism to create both loading patterns. Scratch/occlusal contact characteristics, contact force profiles and the fracture of restored tooth samples were investigated. With bidirectional loading, which imitates the nature of human chewing cycle closer than the unidirectional loading does, the occlusal contact was ovoid in shape whereas a small circular area was observed from the test with unidirectional loading...
2016: Dental Materials Journal
https://www.readbyqxmd.com/read/27902959/kaempferol-a-phytoestrogen-suppressed-triclosan-induced-epithelial-mesenchymal-transition-and-metastatic-related-behaviors-of-mcf-7-breast-cancer-cells
#14
Geum-A Lee, Kyung-Chul Choi, Kyung-A Hwang
As a phytoestrogen, kaempferol is known to play a chemopreventive role inhibiting carcinogenesis and cancer progression. In this study, the influences of triclosan, an anti-bacterial agent recently known for an endocrine disrupting chemical (EDC), and kaempferol on breast cancer progression were examined by measuring their effects on epithelial-mesenchymal transition (EMT) and metastatic-related behaviors of MCF-7 breast cancer cells. Morphological changes of MCF-7 cells were observed, and a wound-healing assay was performed after the treatment of triclosan and kaempferol...
November 22, 2016: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/27901107/how-a-life-like-system-emerges-from-a-simplistic-particle-motion-law
#15
Thomas Schmickl, Martin Stefanec, Karl Crailsheim
Self-structuring patterns can be observed all over the universe, from galaxies to molecules to living matter, yet their emergence is waiting for full understanding. We discovered a simple motion law for moving and interacting self-propelled particles leading to a self-structuring, self-reproducing and self-sustaining life-like system. The patterns emerging within this system resemble patterns found in living organisms. The emergent cells we found show a distinct life cycle and even create their own ecosystem from scratch...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27901023/rhein-and-rhubarb-similarly-protect-the-blood-brain-barrier-after-experimental-traumatic-brain-injury-via-gp91-phox-subunit-of-nadph-oxidase-ros-erk-mmp-9-signaling-pathway
#16
Yang Wang, Xuegong Fan, Tao Tang, Rong Fan, Chunhu Zhang, Zebing Huang, Weijun Peng, Pingping Gan, Xingui Xiong, Wei Huang, Xi Huang
Oxidative stress chiefly contributes to the disruption of the BBB following traumatic brain injury (TBI). The Chinese herbal medicine rhubarb is a promising antioxidant in treating TBI. Here we performed in vivo and in vitro experiments to determine whether rhubarb and its absorbed bioactive compound protected the BBB after TBI by increasing ZO-1 expression through inhibition of gp91(phox) subunit of NADPH oxidase/ROS/ERK/MMP-9 pathway. Rats were subjected to the controlled cortical impact (CCI) model, and primary rat cortical astrocytes were exposed to scratch-wound model...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27900750/-the-management-of-itching-in-burn-patients-during-the-rehabilitation-stage
#17
Shu-Fen Lo, Li-Jung Chang, Ting-Yuan Shiue, Ching-Yi Pan, Hui-Chuan Wu
Pruritus, a distressing symptom that affects burn patients, not only interferes with activities of daily living but also causes disturbed sleep, which has been associated with anxiety, depression, and other types of negative moods. Furthermore, the uncontrolled scratching that often accompanies pruritus causes tissue damage and may damage or destroy the skin graft. In this paper, the authors first describe the definition and mechanism of pruritus and next use itching-assessment tools to identify the triggering factors...
December 2016: Hu Li za Zhi the Journal of Nursing
https://www.readbyqxmd.com/read/27899979/aspirin-inhibits-the-proliferation-and-migration-of-gastric-cancer-cells-in-p53-knockout-mice
#18
Xue-Fu Li, Bing-Zhong Xu, Shi-Zhen Wang
The aim of the present study was to investigate the effect of aspirin on the cell proliferation and migration of gastric cancer cells in p53-knockout mice. Twenty p53(-/-) male mice aged 6 to 7 weeks, with an average weight of 20±3 g were used. The model of gastric cancer was established by the implantation of a mouse forestomach carcinoma cell line, subcutaneously, at the back of the neck, and then the mice were randomly divided into two groups after establishment of the model (control group, n=10; experimental group, n=10)...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27899163/human-fetal-and-adult-epicardial-derived-cells-a-novel-model-to-study-their-activation
#19
Asja T Moerkamp, Kirsten Lodder, Tessa van Herwaarden, Esther Dronkers, Calinda K E Dingenouts, Fredrik C Tengström, Thomas J van Brakel, Marie-José Goumans, Anke M Smits
BACKGROUND: The epicardium, a cell layer covering the heart, plays an important role during cardiogenesis providing cardiovascular cell types and instructive signals, but becomes quiescent during adulthood. Upon cardiac injury the epicardium is activated, which includes induction of a developmental gene program, epithelial-to-mesenchymal transition (EMT) and migration. However, the response of the adult epicardium is suboptimal compared to the active contribution of the fetal epicardium to heart development...
November 29, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27895736/effect-of-silencing-satb1-on-proliferation-invasion-and-apoptosis-of-a549-human-lung-adenocarcinoma-cells
#20
Bo Huang, Hongli Zhou, Siwang Wang, Xian Ping Lang, Xiaodong Wang
The present study aimed to explore the clinical characteristics of special adenine-thymine-rich sequence-binding protein 1 (SATB1) in lung adenocarcinoma and its role in the proliferation, invasion, migration and apoptosis of the lung adenocarcinoma cell line A549. The expression of SATB1 was first studied in tumor tissues of lung adenocarcinoma and adjacent non-tumor tissues. The siRNA green fluorescent protein expression vector of SATB1 was constructed and transfected into the lung adenocarcinoma cell line A549, then a fluorescence microscope was used to study the transfection efficiency...
November 2016: Oncology Letters
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