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https://www.readbyqxmd.com/read/28333142/hydrogen-sulfide-promotes-autophagy-of-hepatocellular-carcinoma-cells-through-the-pi3k-akt-mtor-signaling-pathway
#1
Shanshan S Wang, Yuhan H Chen, Ning Chen, Lijun J Wang, Dexi X Chen, Honglei L Weng, Steven Dooley, Huiguo G Ding
Hydrogen sulfide (H2S), in its gaseous form, plays an important role in tumor carcinogenesis. This study investigated the effects of H2S on the cell biological functions of hepatocellular carcinoma (HCC). HCC cell lines, HepG2 and HLE, were treated with NaHS, a donor of H2S, and rapamycin, a classic autophagy inducer, for different lengths of time. Western blotting, immunofluorescence, transmission electron microscopy (TEM), scratch assay, CCK-8 and flow cytometric analysis were carried out to examine the effects of H2S on HCC autophagy, cell behavior and PI3K/Akt/mTOR signaling...
March 23, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28332208/itchy-channels-and-where-to-find-them
#2
Nikita Gamper
Itch (pruritus) is an unpleasant sensory experience that triggers a desire to scratch. It is induced by activation of a subset of cutaneous C fibers in response to environmental irritants and some endogenous mediators (such as histamine) This article is protected by copyright. All rights reserved.
March 23, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28332151/blockade-of-neuroglobin-reduces-protection-of-conditioned-medium-from-human-mesenchymal-stem-cells-in-human-astrocyte-model-t98g-under-a-scratch-assay
#3
Eliana Baez-Jurado, Gina Guio Vega, Gjumrakch Aliev, Vadim V Tarasov, Paula Esquinas, Valentina Echeverria, George E Barreto
Previous studies have indicated that paracrine factors (conditioned medium) increase wound closure and reduce reactive oxygen species in a traumatic brain injury in vitro model. Although the beneficial effects of conditioned medium from human adipose tissue-derived mesenchymal stem cells (hMSCA-CM) have been previously suggested for various neurological diseases, their actions on astrocytic cells are not well understood. In this study, we have explored the effect of hMSCA-CM on human astrocyte model (T98G cells) subjected to scratch assay...
March 22, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28331343/silencing-trim59-inhibits-invasion-migration-and-epithelial-to-mesenchymal-transition-via-tgf-%C3%AE-smad2-3-signaling-pathway-in-bladder-cancer-cells
#4
Wei Chen, Kai Zhao, Chenkui Miao, Aiming Xu, Jianzhong Zhang, Jundong Zhu, Shifeng Su, Zengjun Wang
The evolutionarily conserved genes that encode the tripartite motif (TRIM) protein family are involved in various biological processes, including cellular immunity, inflammatory reaction, antiviral activity, and tumor progression. One member of this protein family, Trim59, has been reported as a novel biomarker for the occurrence and progression of multiple human carcinomas, such as lung cancer, gastric cancer, cervical cancer, and osteosarcoma. However, little is known about the relationship between Trim59 and bladder carcinogenesis...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28327550/significant-role-of-collagen-xvii-and-integrin-%C3%AE-4-in-migration-and-invasion-of-the-less-aggressive-squamous-cell-carcinoma-cells
#5
Jyri M Moilanen, Stefanie Löffek, Nina Kokkonen, Sirpa Salo, Juha P Väyrynen, Tiina Hurskainen, Aki Manninen, Pilvi Riihilä, Ritva Heljasvaara, Claus-Werner Franzke, Veli-Matti Kähäri, Tuula Salo, Markus J Mäkinen, Kaisa Tasanen
Collagen XVII and integrin α6β4 have well-established roles as epithelial adhesion molecules. Their binding partner laminin 332 as well as integrin α6β4 are largely recognized to promote invasion and metastasis in various cancers, and collagen XVII is essential for the survival of colon and lung cancer stem cells. We have studied the expression of laminin γ2, collagen XVII and integrin β4 in tissue microarray samples of squamous cell carcinoma (SCC) and its precursors, actinic keratosis and Bowen's disease...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28326930/effects-of-jwh015-in-cytokine-secretion-in-primary-human-keratinocytes-and-fibroblasts-and-its-suitability-for-topical-transdermal-delivery
#6
Alicia Bort, Perla A Alvarado-Vazquez, Carolina Moracho-Vilrriales, Kristopher G Virga, Giuseppe Gumina, Alfonso Romero-Sandoval, Scott Asbill
Background JWH015 is a cannabinoid (CB) receptor type 2 agonist that produces immunomodulatory effects. Since skin cells play a key role in inflammatory conditions and tissue repair, we investigated the ability of JWH015 to promote an anti-inflammatory and pro-wound healing phenotype in human primary skin cells. Methods Human primary keratinocytes and fibroblasts were stimulated with lipopolysaccharide. The mRNA expression of cannabinoid receptors was determined using RT-PCR. The effects of JWH015 (0.05, 0...
January 2017: Molecular Pain
https://www.readbyqxmd.com/read/28326125/determination-of-the-wound-healing-potentials-of-medicinal-plants-historically-used-in-ghana
#7
Sara H Freiesleben, Jens Soelberg, Nils T Nyberg, Anna K Jäger
The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice. The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28324043/reduced-transforming-growth-factor-beta-activity-in-the-endometrium-of-women-with-heavy-menstrual-bleeding
#8
J A Maybin, L Boswell, V J Young, W C Duncan, Hod Critchley
Context: Heavy menstrual bleeding (HMB) is common and incapacitating. Aberrant menstrual endometrial repair may result in HMB. The TGFβ superfamily contributes to tissue repair, but its role in HMB is unknown. Objective: We hypothesised that TGFβ1 is important for endometrial repair and women with HMB have aberrant TGFβ1 activity at menses. Participants/Setting: Endometrial biopsies were collected from women and menstrual blood loss objectively measured (HMB >80ml/cycle, normal menstrual bleeding, NMB <80ml)...
January 3, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28323840/htc-vive-mevislab-integration-via-openvr-for-medical-applications
#9
Jan Egger, Markus Gall, Jürgen Wallner, Pedro Boechat, Alexander Hann, Xing Li, Xiaojun Chen, Dieter Schmalstieg
Virtual Reality, an immersive technology that replicates an environment via computer-simulated reality, gets a lot of attention in the entertainment industry. However, VR has also great potential in other areas, like the medical domain, Examples are intervention planning, training and simulation. This is especially of use in medical operations, where an aesthetic outcome is important, like for facial surgeries. Alas, importing medical data into Virtual Reality devices is not necessarily trivial, in particular, when a direct connection to a proprietary application is desired...
2017: PloS One
https://www.readbyqxmd.com/read/28322716/bartonella-associated-transverse-myelitis
#10
Parham Sendi, Cedric Hirzel, Andreas Bloch, Urs Fischer, Natalie Jeannet, Livia Berlinger, Heinz Krestel
Each year in the United States, 500 patients are hospitalized for cat-scratch disease, caused by Bartonella henselae infection. We report a case of rare but serious neurologic B. henselae infection. When typical features of cat-scratch disease occur with neurologic findings, Bartonella infection should be suspected and diagnostic testing should be performed.
April 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28316077/development-of-resistance-to-serotonin-induced-itch-in-bile-duct-ligated-mice
#11
Sattar Ostadhadi, Nazgol-Sadat Haddadi, Arash Foroutan, Ehsan Azimi, Sarina Elmariah, Ahmad-Reza Dehpour
Cholestatic itch can be severe and significantly impair the quality of life of patients. Serotonin system is implicated in cholestatic itch; however, the pruritogenic properties of serotonin have not been evaluated in cholestatic mice. Here, we investigated the serotonin-induced itch in cholestatic mice which was induced by bile duct ligation (BDL). Serotonin, sertraline or saline were administered intradermally to the rostral back area in BDL and sham-operated (SHAM) mice, and the scratching behavior was videotaped for 1 hour...
March 18, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28315466/innovative-approach-for-identifying-root-causes-of-glass-defects-in-sterile-drug-product-manufacturing
#12
Lukas Eberle, Alexander Svensson, Andreas Graser, Joerg Luemkemann, Hirokazu Sugiyama, Rainer Schmidt, Konrad Hungerbuehler
In sterile drug product manufacturing, scratched and broken glass containers (i.e., vials) cause product losses, glass particles, equipment contamination and additional cleaning efforts. However, mechanical resistance and exposure of vials to mechanical stress are not sufficiently understood, and no systematic approach for reducing glass-related losses is established. Manufacturers may tackle glass-related losses more rationally if (i) frequencies for inflicting disqualifying damages to drug product containers are known for given forces, (ii) actual exposure in industrial filling lines is quantified and (iii) process enhancements are derived based on collected information...
March 14, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28314729/efficacy-of-a-sonicating-swab-for-removal-and-capture-of-listeria-monocytogenes-in-biofilm-on-stainless-steel
#13
Tobyn A Branck, Matthew J Hurley, Gianna N Prata, Christina A Crivello, Patrick J Marek
Listeria monocytogenes is of great concern in food processing facilities because it persists in biofilms, potentiating biotransfer. Stainless steel is commonly used for food contact surfaces and transport containers. L. monocytogenes biofilm on stainless steel served as a model system for surface sampling to test the performance of a sonicating swab in comparison to a standard cotton swab. Swab performance and consistency were determined using total viable counts. Stainless steel coupons sampled with both swabs were examined with SEM to visualize biofilms and surface structures (i...
March 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28306533/ros-mediated-induction-of-apoptosis-by-benzoquinone-embelin-in-human-colon-adenocarcinoma-cells-ht-29
#14
Kodandaram Sumalatha, Mohan Gowda, Sreepriya Meenakshisundaram
Background Embelin is a benzoquinone reported to possess anticancer activity in several in vivo and in vitro models of carcinogenesis, especially hematopoietic and prostate malignancy. A detailed investigation on the influence of embelin on epithelial malignancy model system, especially colon adenocarcinoma, is lacking. The objective of the current study is to investigate the antiproliferative, antiinvasive and proapoptotic potential of embelin on colon adenocarcinoma cell line HT-29. Methods The effect of embelin (35 µg/mL for 24 h) on cell proliferation was assessed by Sulforhodamine B assay and bromodeoxyuridine incorporation test, antiinvasive effect by Boyden chamber assay and scratch assay...
March 17, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28302576/adolescent-chronic-escalating-morphine-administration-induces-long-lasting-changes-in-tolerance-and-dependence-to-morphine-in-rats
#15
Hamed Salmanzadeh, Hossein Azizi, Saeed Semnanian
Adolescence is a gradual period of transition from childhood to adulthood. It is considered as a sensitive developmental time point that long lasting changes occur in the brain. The present study examined adolescent chronic escalating morphine administration on morphine tolerance and dependence in adulthood. Adolescent male Wistar rats (30days old) were administered increasing doses of morphine (2.5 to 25mg/kg, s.c.) every 12h, for 10days. Control rats received saline according to the same protocol. Thereafter, during adulthood (65-75days old), tolerance to antinociceptive effect of morphine was induced by subcutaneous injection of 3mg/kg morphine, once a day for 7days...
March 13, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28301220/periostin-promotes-migration-proliferation-and-differentiation-of-human-periodontal-ligament-mesenchymal-stem-cells
#16
Ziqiang Wu, Wenyong Dai, Pei Wang, Xiaozhen Zhang, Yi Tang, Lin Liu, Qiaona Wang, Ming Li, Chunbo Tang
PURPOSE: Periostin (POSTN) is critical to bone and dental tissue morphogenesis, postnatal development, and maintenance, however, its roles in tissue repair and regeneration mediated by human periodontal ligament mesenchymal stem cells (PDLSCs) remain unclear. The present study was designed to evaluate the effects of POSTN on hPDLSCs in vitro. MATERIALS AND METHODS: hPDLSCs were isolated and characterized by their expression of the cell surface markers CD44, CD90, CD105, CD34, and CD45...
March 16, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28300683/expression-of-hsp70-reveals-significant-differences-between-fin-regeneration-and-inflammation-in-paramisgurnus-dabryanus
#17
Li Li, Linlin Wang, Jingya He, Zhongjie Chang
Hsp70 is the most strongly induced in response to various cellular stresses and a good candidate for investigating its role in tissue injury. We firstly cloned full-length cDNA of hsp70 from Paramisgurnus dabryanus (PdHsp70) by RACE method (GenBank: KP402408). Then regeneration and inflammation of fin were established by amputation and scratch respectively. Quantitative RT-PCR detected the PdHsp70 began to increase rapidly its expression at 1 days post amputation (dpa) and reached the peak at 2 dpa during fin regeneration...
March 11, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28295731/flexible-hard-coating-glass-like-wear-resistant-yet-plastic-like-compliant-transparent-protective-coating-for-foldable-displays
#18
Gwang-Mun Choi, Jungho Jin, Dahye Shin, Yun Hyeok Kim, Ji-Hoon Ko, Hyeon-Gyun Im, Junho Jang, Dongchan Jang, Byeong-Soo Bae
A flexible hard coating for foldable displays is realized by the highly cross-linked siloxane hybrid using structure-property relationships in organic-inorganic hybridization. Glass-like wear resistance, plastic-like flexibility, and highly elastic resilience are demonstrated together with outstanding optical transparency. It provides a framework for the application of siloxane hybrids in protective hard coatings with high scratch resistance and flexibility for foldable displays.
March 15, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28295043/chlorin-e6-mediated-photodynamic-inactivation-for-multidrug-resistant-pseudomonas-aeruginosa-keratitis-in-mice-in-vivo
#19
Ming-Feng Wu, Mona Deichelbohrer, Thomas Tschernig, Matthias W Laschke, Nóra Szentmáry, Dirk Hüttenberger, Hans-Jochen Foth, Berthold Seitz, Markus Bischoff
Following corneal epithelium scratches, mouse corneas were infected with the multidrug resistant (MDR) P. aeruginosa strain PA54. 24 hours later, 0% (for control group), 0.01%, 0.05% or 0.1% Chlorin e6 (Ce6), a second generation photosensitizer derived from chlorophyll, was combined with red light, for photodynamic inactivation (PDI). 1 hour or 2 days later, entire mouse eyes were enucleated and homogenized for counting colony forming units (CFU) of P. aeruginosa. For comparison, 0.1% Ce6 mediated PDI was started at 12 hours post infection, and 0...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28293440/the-diagnosis-and-treatment-of-multiple-factitious-oral-ulcers-in-a-6-year-old-boy
#20
Priscilla Santana Pinto Gonçalves, Daniela Alejandra Cusicanqui Mendez, Paulo Sérgio da Silva Santos, José Humberto Damante, Daniela Rios, Thiago Cruvinel
Factitious ulcers are characterized by self-inflicted lesions with multifactorial origin. These lesions are frequently found in head, neck, and hands. This report shows a 6-year-old boy diagnosed with factitious oral ulcers that occurred after the self-biting of buccal vestibule and nail-scratching of gingival tissue. Clinically, a significant swelling was observed, hard on palpation, located at the right lower third of the face, next to the posterior area of the mandible. In the intraoral examination, ulcers at different healing stages were noted on the swelling area...
2017: Case Reports in Dentistry
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