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https://www.readbyqxmd.com/read/28915927/a-decade-of-research-into-classical-swine-fever-marker-vaccine-cp7_e2alf-suvaxyn-%C3%A2-csf-marker-a-review-of-vaccine-properties
#1
REVIEW
Sandra Blome, Kerstin Wernike, Ilona Reimann, Patricia König, Claudia Moß, Martin Beer
Due to its impact on animal health and pig industry, classical swine fever (CSF) is still one of the most important viral diseases of pigs. To control the disease, safe and highly efficacious live attenuated vaccines exist for decades. However, until recently, the available live vaccines did not allow a serological marker concept that is essentially important to circumvent long-term trade restrictions. In 2014, a new live attenuated marker vaccine, Suvaxyn(®) CSF Marker (Zoetis), was licensed by the European Medicines Agency...
September 15, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28859602/corrigendum
#2
(no author information available yet)
Sayler KA, Bigelow T, Koster LG, Swenson S, Bounds C, Hernández F, Wisely SM. Development of a rapid, simple, and specific real-time PCR assay for detection of pseudorabies viral DNA in domestic swine herds. J Vet Diagn Invest 2017;29:522-528. (Original doi:10.1177/1040638717706593). In the article titled "Development of a rapid, simple, and specific real-time PCR assay for detection of pseudorabies viral DNA in domestic swine herds" by Katherine A. Sayler et al., the Acknowledgements section, should read as follows: We thank all of the researchers who provided isolates or DNA samples of PRV, including C Romero at the University of Florida, College of Veterinary Medicine (U...
September 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28790203/the-in-feed-antibiotic-carbadox-induces-phage-gene-transcription-in-the-swine-gut-microbiome
#3
Timothy A Johnson, Torey Looft, Andrew J Severin, Darrell O Bayles, Daniel J Nasko, K Eric Wommack, Adina Howe, Heather K Allen
Carbadox is a quinoxaline-di-N-oxide antibiotic fed to over 40% of young pigs in the United States that has been shown to induce phage DNA transduction in vitro; however, the effects of carbadox on swine microbiome functions are poorly understood. We investigated the in vivo longitudinal effects of carbadox on swine gut microbial gene expression (fecal metatranscriptome) and phage population dynamics (fecal dsDNA viromes). Microbial metagenome, transcriptome, and virome sequences were annotated for taxonomic inference and gene function by using FIGfam (isofunctional homolog sequences) and SEED subsystems databases...
August 8, 2017: MBio
https://www.readbyqxmd.com/read/28780258/behavior-of-antibiotic-resistance-genes-during-co-composting-of-swine-manure-with-chinese-medicinal-herbal-residues
#4
Li Zhang, Jie Gu, Xiaojuan Wang, Wei Sun, Yanan Yin, Yixin Sun, Aiyun Guo, Xiaxia Tuo
Swine manure is considered to be a reservoir for antibiotic resistance genes (ARGs) but little is known about the variations in ARGs during the co-composting of swine manure with Chinese medicinal herbal residues (CMHRs). Thus, this study explored the effects of CMHRs on the variations in ARGs during co-composting with swine manure. The results showed that CMHRs could reduce effectively most of the targeted ARGs (0.18-2.82logs) and mobile genetic elements (MGEs) (0.47-3.34logs). The correlations indicated that CMHRs might decrease the spread of ARGs via horizontal gene transfer...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28757016/characterization-of-corynebacterium-diphtheriae-c-confusum-and-c-amycolatum-isolated-from-sows-with-genitourinary-infection
#5
André P Poor, Luisa Z Moreno, Carlos E C Matajira, Beatriz M Parra, Vasco T M Gomes, Ana Paula S Silva, Mauricio C Dutra, Ana Paula G Christ, Mikaela R F Barbosa, Maria Inês Z Sato, Andrea M Moreno
Porcine Corynebacterium infection is still poorly studied, even though the pig has been described as an asymptomatic carrier of Corynebacterium species, including the zoonotic species C. ulcerans, C. confusum and C. amycolatum. Here we present the identification, molecular and antimicrobial susceptibility characterization of coryneform bacteria isolated from sows with urinary tract infection. C. diphtheriae, C. confusum and C. amycolatum were isolated from sows with urinary infection and metritis. Corynebacterium species were identified by MALDI-TOF MS and confirmed by 16S rRNA and rpoB sequencing...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28699384/preclinical-safety-evaluation-of-allogeneic-ips-cell-based-therapy-in-a-swine-model-of-myocardial-infarction
#6
Carolina Gálvez-Montón, Carolina Soler-Botija, Oriol Iborra-Egea, Idoia Diaz-Guemes, Mercè Martí, Olalla Iglesias-Garcia, Cristina Prat-Vidal, Verónica Crisóstomo, Aida Llucià-Valldeperas, Isaac Perea-Gil, Santiago Roura, Francisco Miguel Sanchez Margallo, Ángel Raya, Antoni Bayés-Genís
The combination of biomatrices and induced pluripotent stem cell (iPSC) derivatives to aid repair and myocardial scar formation may soon become a reality for cardiac regenerative medicine. However, the tumor risk associated to residual undifferentiated cells remains an important safety concern of iPSC-based therapies. This concern is not satisfactorily addressed in xenotransplantation, which require immune suppression of the transplanted animal. Here, we assessed the safety of transplanting undifferentiated iPSC in an allogeneic setting...
July 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28658278/antimicrobial-use-surveillance-in-broiler-chicken-flocks-in-canada-2013-2015
#7
Agnes Agunos, David F Léger, Carolee A Carson, Sheryl P Gow, Angelina Bosman, Rebecca J Irwin, Richard J Reid-Smith
There is a paucity of data on the reason for and the quantity of antimicrobials used in broiler chickens in Canada. To address this, the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) implemented surveillance of antimicrobial use (AMU) and antimicrobial resistance (AMR) in broiler chicken flocks in 2013. Shortly after this (2014), the poultry industry banned the preventive use of ceftiofur in broiler chickens. The objectives of this analysis were to describe antimicrobial use (AMU) in Canadian broiler chickens between 2013 and 2015 (n = 378 flocks), compare these results to other animal species in Canada, to highlight the utility of farm surveillance data to evaluate the impact of a policy change, and to explore how different antimicrobial use metrics might affect data interpretation and communication...
2017: PloS One
https://www.readbyqxmd.com/read/28603717/assessment-of-the-risk-to-public-health-due-to-use-of-antimicrobials-in-pigs-an-example-of-pleuromutilins-in-denmark
#8
Lis Alban, Johanne Ellis-Iversen, Margit Andreasen, Jan Dahl, Ute W Sönksen
Antibiotic consumption in pigs can be optimized by developing treatment guidelines, which encourage veterinarians to use effective drugs with low probability of developing resistance of importance for human health. In Denmark, treatment guidelines for use in swine production are currently under review at the Danish Veterinary and Food Administration. Use of pleuromutilins in swine has previously been associated with a very low risk for human health. However, recent international data and sporadic findings of novel resistance genes suggest a change of risk...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28596049/modern-science-for-better-quality-control-of-medicinal-products-towards-global-harmonization-of-3rs-in-biologicals-the-report-of-an-epaa-workshop
#9
Katrin Schutte, Anna Szczepanska, Marlies Halder, Klaus Cussler, Ursula G Sauer, Catrina Stirling, Sylvie Uhlrich, Iwona Wilk-Zasadna, David John, Martin Bopst, Joerg Garbe, Harrie L Glansbeek, Robin Levis, Pieter-Jan Serreyn, Dean Smith, Paul Stickings
This article summarizes the outcome of an international workshop organized by the European Partnership for Alternative Approaches to Animal Testing (EPAA) on Modern science for better quality control of medicinal products: Towards global harmonization of 3Rs in biologicals. As regards the safety testing of biologicals, the workshop participants agreed to actively encourage the deletion of abnormal toxicity tests and target animal batch safety tests from all relevant legal requirements and guidance documents (country-specific guidelines, pharmacopoeia monographs, WHO recommendations)...
June 5, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28583756/thrombelastography-indicates-limitations-of-animal-models-of-trauma-induced-coagulopathy
#10
Gregory R Stettler, Ernest E Moore, Hunter B Moore, Peter J Lawson, Miguel Fragoso, Geoffrey R Nunns, Christopher C Silliman, Anirban Banerjee
BACKGROUND: Thrombelastography (TEG) has been used to characterize the coagulation changes associated with injury and shock. Animal models developed to investigate trauma-induced coagulopathy (TIC) have failed to produce excessive bleeding. We hypothesize that a native TEG will demonstrate marked differences in humans compared with these experimental models, which explains the difficulties in reproducing a clinically relevant coagulopathy in animal models. METHODS: Whole blood was collected from 138 healthy human volunteers, 25 swine and 66 Sprague-Dawley rats before experimentation...
September 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28559706/flap-surgical-techniques-for-incisional-hernia-recurrences-a-swine-experimental-model
#11
Florina Popa, Filip Ardelean, Cosmin Pestean, Robert Purdoiu, Oana Rosca, Alexandru Georgescu
BACKGROUND AND AIM: In the age of synthetic prostheses most of hernia studies include a careful examination of the various types of prosthesis, their characteristics and their repair indications. Biological prostheses are also beginning to draw attention. But in terms of recurrence especially for poor or developing countries, the discussion is different, due to their high cost which makes them difficult to afford. In this article we present new flap reconstruction techniques for the reconstruction of the abdominal wall versus mesh repair, applied on swine models, outline the results of each technique, and specify the indications for their use...
2017: Clujul Medical (1957)
https://www.readbyqxmd.com/read/28527823/antiviral-escin-derivatives-from-the-seeds-of-aesculus-turbinata-blume-japanese-horse-chestnut
#12
Ji Won Kim, Thi-Kim-Quy Ha, Hyomoon Cho, Eunhee Kim, Sang Hee Shim, Jun-Li Yang, Won Keun Oh
Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and high fatality of piglets, influencing the swine industry. Japanese horse chestnut (seed of Aesculus turbinata) contains many saponin mixtures, called escins, and has been used for a long time as a traditional medicinal plant. Structure-activity relationship (SAR) studies on escins have revealed that acylations at C-21 and C-22 with angeloyl or tigloyl groups were important for their cytotoxic effects. However, the strong cytotoxicity of escins makes them hard to utilize for other diseases and to develop as nutraceuticals...
July 1, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28497622/non-contrast-enhanced-t1-weighted-mri-of-myocardial-radiofrequency-ablation-lesions
#13
Michael A Guttman, Susumu Tao, Sarah Fink, Aravindan Kolandaivelu, Henry R Halperin, Daniel A Herzka
PURPOSE: To demonstrate imaging of radiofrequency ablation lesions with non-contrast-enhanced T1 -weighted (T1w) MRI. METHODS: Fifteen swine underwent left ventricular ablation followed by MRI using different preparations: endocardial or epicardial ablation of naïve animal, or endocardial ablation of animal with myocardial infarction. Lesion imaging was performed using free-breathing, non-contrast-enhanced, T1w sequence with long inversion time (TI). Also acquired were T1 maps and delayed contrast-enhanced (DCE) imaging...
May 11, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28488326/acute-pressure-changes-in-the-brain-are-correlated-with-mr-elastography-stiffness-measurements-initial-feasibility-in-an-in-vivo-large-animal-model
#14
Arvin Arani, Hoon-Ki Min, Nikoo Fattahi, Nicholas M Wetjen, Joshua D Trzasko, Armando Manduca, Clifford R Jack, Kendall H Lee, Richard L Ehman, John Huston
PURPOSE: The homeostasis of intracranial pressure (ICP) is of paramount importance for maintaining normal brain function. A noninvasive technique capable of making direct measurements of ICP currently does not exist. MR elastography (MRE) is capable of noninvasively measuring brain tissue stiffness in vivo, and may act as a surrogate to measure ICP. The objective of this study was to investigate the impact of changing ICP on brain stiffness using MRE in a swine model. METHODS: Baseline MRE measurements were obtained, and then catheters were surgically placed into the left and right lateral ventricles of three animals...
May 9, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28452348/wincs-harmoni-closed-loop-dynamic-neurochemical-control-of-therapeutic-interventions
#15
Kendall H Lee, J Luis Lujan, James K Trevathan, Erika K Ross, John J Bartoletta, Hyung Ook Park, Seungleal Brian Paek, Evan N Nicolai, Jannifer H Lee, Hoon-Ki Min, Christopher J Kimble, Charles D Blaha, Kevin E Bennet
There has been significant progress in understanding the role of neurotransmitters in normal and pathologic brain function. However, preclinical trials aimed at improving therapeutic interventions do not take advantage of real-time in vivo neurochemical changes in dynamic brain processes such as disease progression and response to pharmacologic, cognitive, behavioral, and neuromodulation therapies. This is due in part to a lack of flexible research tools that allow in vivo measurement of the dynamic changes in brain chemistry...
April 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28382726/improved-dark-blood-late-gadolinium-enhancement-db-lge-imaging-using-an-optimized-joint-inversion-preparation-and-t2-magnetization-preparation
#16
Tamer A Basha, Maxine C Tang, Connie Tsao, Cory M Tschabrunn, Elad Anter, Warren J Manning, Reza Nezafat
PURPOSE: To develop a dark blood-late gadolinium enhancement (DB-LGE) sequence that improves scar-blood contrast and delineation of scar region. METHODS: The DB-LGE sequence uses an inversion pulse followed by T2 magnetization preparation to suppress blood and normal myocardium. Time delays inserted after preparation pulses and T2 -magnetization-prep duration are used to adjust tissue contrast. Selection of these parameters was optimized using numerical simulations and phantom experiments...
April 5, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28370375/radiofrequency-heating-studies-on-anesthetized-swine-using-fractionated-dipole-antennas-at-10-5-t
#17
Yiğitcan Eryaman, Russell L Lagore, M Arcan Ertürk, Lynn Utecht, Patrick Zhang, Angel Torrado-Carvajal, Esra Abaci Türk, Lance DelaBarre, Gregory J Metzger, Gregor Adriany, Kâmil Uğurbil, J Thomas Vaughan
PURPOSE: To validate electromagnetic and thermal simulations with in vivo temperature measurements, and to demonstrate a framework that can be used to predict temperature increase caused by radiofrequency (RF) excitation with dipole transmitter arrays. METHODS: Dipole arrays were used to deliver RF energy in the back/neck region of the swine using different RF excitation patterns (n = 2-4 per swine) for heating. The temperature in anesthetized swine (n = 3) was measured using fluoroscopic probes (n = 12) and compared against thermal modeling from animal-specific electromagnetic simulations...
March 31, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28342176/investigating-the-physiological-effects-of-10-5-tesla-static-field-exposure-on-anesthetized-swine
#18
Yigitcan Eryaman, Patrick Zhang, Lynn Utecht, Kivanc Kose, Russell L Lagore, Lance DelaBarre, Jeramy Kulesa, Lynn E Eberly, Gregor Adriany, Tinen L Iles, Paul A Iaizzo, J Thomas Vaughan, Kamil Ugurbil
PURPOSE: In this work, we investigated the relative effects of static magnetic field exposure (10.5 Tesla [T]) on two physiological parameters; blood pressure (BP) and heart rate (HR). METHODS: In vivo, we recorded both BP and HR in 4 swine (3 female, 1 male) while they were positioned within a 10.5T magnet. All measurements were performed invasively within these anesthetized animals by the placement of pressure catheters into their carotid arteries. RESULTS: We measured average increases of 2...
March 25, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28319826/oil-based-adjuvants-delivered-intradermally-induce-high-primary-igg2-immune-response-in-swine
#19
Zrinka Oreskovic, Hana Kudlackova, Josef Krejci, Katerina Nechvatalova, Martin Faldyna
The effects of intradermal application of antigen with or without different adjuvants and activation of immune response are presented in this study. Six groups of six piglets each were immunized with keyhole limpet haemocyanin (KLH) antigen in combination with aluminium hydroxide or oil-based adjuvants (complete and incomplete Freund's adjuvants, Montanide ISA 206 and Emulsigen). IgG1 and IgG2 levels in sera were measured by KLH-specific ELISA. Interestingly, oil-based adjuvants induced high primary IgG2 response, suggesting the Th1 lymphocyte polarization...
March 11, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28287510/adsc-sheet-transplantation-to-prevent-stricture-after-extended-esophageal-endoscopic-submucosal-dissection
#20
Guillaume Perrod, Laetitia Pidial, Sophie Camilleri, Alexandre Bellucci, Amaury Casanova, Thomas Viel, Bertrand Tavitian, Chirstophe Cellier, Olivier Clément, Gabriel Rahmi
In past years, the cell-sheet construct has spurred wide interest in regenerative medicine, especially for reconstructive surgery procedures. The development of diversified technologies combining adipose tissue-derived stromal cells (ADSCs) with various biomaterials has led to the construction of numerous types of tissue-engineered substitutes, such as bone, cartilage, and adipose tissues from rodent, porcine, or human ADSCs. Extended esophageal endoscopic submucosal dissection (ESD) is responsible for esophageal stricture formation...
February 10, 2017: Journal of Visualized Experiments: JoVE
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