Richard Searfoss, Punit Shah, Kennedy Ofori-Mensa, Valerie Bussberg, Vladimir Tolstikov, Bennett Greenwood, Hongyan Li, Kris Richardson, Gregory M Miller, Corinne DeCicco, Elder Granger, Leonardo O Rodrigues, Eric M Grund, A James Moser, Rangaprasad Sarangarajan, Niven R Narain, Michael A Kiebish
Cancer biomarker discovery is critically dependent on the integrity of biofluid and tissue samples acquired from study participants. Multi-omic profiling of candidate protein, lipid, and metabolite biomarkers is confounded by timing and fasting status of sample collection, participant demographics and treatment exposures of the study population. Contamination by hemoglobin, whether caused by hemolysis during sample preparation or underlying red cell fragility, contributes 0-10 g/L of extraneous protein to plasma, serum, and Buffy coat samples and may interfere with biomarker detection and validation...
January 24, 2022: Scientific Reports